Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.003
A. Sahara, F. Ibrahim, M. Massi, A. Yasmon
Besides human papillomavirus (HPV), the cervical carcinogenesis is also affected by many risk factors including pathogenic bacteria such as Chlamydia trachomatis (CT), Mycoplasma spp. (MS), Ureaplasma urealyticum (UU), and U. parvum (UP) infections. Thus, we studied the bacterial infections for 68 patients with cervical cancerous cases and other cervical problems. Cervical swab samples were collected by specialist doctors and tested for the pathogenic bacteria by real time polymerase chain reaction (rPCR) and HPV by conventional PCR. Of 68 patients, 22 were diagnosed as cervical cancer and 46 were diagnosed as other cervical problems. All patients with cervical cancer were HPV positive, while the patients with other cervical problems were HPV negative. Of 22 HPV positive-cervical cancer patients, 7 (31.82%), 6 (27.27%), 3 (13.64%) were positive for MS, UU, and UP, respectively. None of the patients was CT positive. For 46 HPV negative-other cervical problem patients, 6 (13.04%), 12 (26.09%), 20 (43.48%), and 22 (47.83%) were positive for CT, MS, UU, and UP, respectively. There was no association of CT, MS, and UU infections with the HPV positive-cervical cancer patients (p >0.05). However, there is a negative correlation between UP infection and the HPV negative-other cervical problem patients (p<0.05).
除了人乳头瘤病毒(HPV)外，宫颈癌的发生还受到许多危险因素的影响，包括致病菌，如沙眼衣原体(CT)、支原体(MS)、解脲支原体(UU)和细小脲原体(UP)感染。因此，我们对68例宫颈癌及其他宫颈疾病患者的细菌感染进行了研究。专科医生采集宫颈拭子样本，采用实时聚合酶链反应(real - time polymerase chain reaction, rPCR)检测致病菌，采用常规PCR检测HPV。在68名病人中，22名被诊断为子宫颈癌，46名被诊断为其他子宫颈问题。所有宫颈癌患者均为HPV阳性，而其他宫颈问题患者均为HPV阴性。22例HPV阳性宫颈癌患者中，MS阳性7例(31.82%)，UU阳性6例(27.27%)，UP阳性3例(13.64%)。所有患者均为CT阳性。46例HPV阴性-其他宫颈问题患者中，CT、MS、UU、UP分别阳性6例(13.04%)、12例(26.09%)、20例(43.48%)、22例(47.83%)。CT、MS、UU感染与HPV阳性宫颈癌患者无相关性(p >0.05)。而UP感染与HPV阴性-其他宫颈问题患者呈负相关(p<0.05)。
{"title":"Association of Chlamydia trachomatis, Mycoplasma spp., Ureaplasma urealyticum and U. parvum with Human Papillomavirus in Patients with Cervical Cancer","authors":"A. Sahara, F. Ibrahim, M. Massi, A. Yasmon","doi":"10.2991/absr.k.210810.003","DOIUrl":"https://doi.org/10.2991/absr.k.210810.003","url":null,"abstract":"Besides human papillomavirus (HPV), the cervical carcinogenesis is also affected by many risk factors including pathogenic bacteria such as Chlamydia trachomatis (CT), Mycoplasma spp. (MS), Ureaplasma urealyticum (UU), and U. parvum (UP) infections. Thus, we studied the bacterial infections for 68 patients with cervical cancerous cases and other cervical problems. Cervical swab samples were collected by specialist doctors and tested for the pathogenic bacteria by real time polymerase chain reaction (rPCR) and HPV by conventional PCR. Of 68 patients, 22 were diagnosed as cervical cancer and 46 were diagnosed as other cervical problems. All patients with cervical cancer were HPV positive, while the patients with other cervical problems were HPV negative. Of 22 HPV positive-cervical cancer patients, 7 (31.82%), 6 (27.27%), 3 (13.64%) were positive for MS, UU, and UP, respectively. None of the patients was CT positive. For 46 HPV negative-other cervical problem patients, 6 (13.04%), 12 (26.09%), 20 (43.48%), and 22 (47.83%) were positive for CT, MS, UU, and UP, respectively. There was no association of CT, MS, and UU infections with the HPV positive-cervical cancer patients (p >0.05). However, there is a negative correlation between UP infection and the HPV negative-other cervical problem patients (p<0.05).","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125139772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.004
S. Afrida, K. Willard, Lukman, Y. Tamai
Indonesian mushroom farmers commonly produce Pleurotus ostreatus spawn in bottles. Bottles have several disadvantages: unsterilized lids, large weights, undetectable bacterial contaminations, and difficulties in removing the rest of mycelia around the edge of the bottle. Farmers rarely use plastic bags due to unfamiliarity. Therefore, in this study, we investigate growth of P. ostreatus spawn in heat-tolerant plastic bags using three different substrates (corn, rice, and a mixture of corn and rice). The physiological and morphological properties of mycelia growth on substrates were assessed, including growth rate, type of mycelium, and colony formation. Grain moisture is an important factor to get a successful spawn. We estimated the suitable water content of corn and rice in plastic bags was 50% and 27%, respectively. Inoculation of inoculum from potato dextrose agar (PDA) into grains was called first-generation spawn (G1). The corn medium showed the fastest growth (2 weeks), followed by the mixture of corn and rice (4 weeks), followed by the rice medium (6 weeks). Inoculation of G1 into another grain was called second-generation spawn (G2). Inoculation of G1 into G2 from corn to corn mycelia grew for 1 week, from the mixture of corn and rice on the same substrate for 2 weeks, and the slowest growth was in mycelium from rice to rice substrate for 4 weeks. An application of spawn to mushroom cultivation showed that farmers are able to expand G2 into 30-50 of sawdust fruiting body substrate. From the three types of spawn, farmers reported that they obtained good mushroom yield when the mixture of corn and rice was used as spawn. This study may be useful for new methods to generate spawn.
{"title":"An Investigation of Spawn Growth of Pleurotus ostreatus in Heat-Tolerant Plastic Bags Using Rice and Corn as Substrates","authors":"S. Afrida, K. Willard, Lukman, Y. Tamai","doi":"10.2991/absr.k.210810.004","DOIUrl":"https://doi.org/10.2991/absr.k.210810.004","url":null,"abstract":"Indonesian mushroom farmers commonly produce Pleurotus ostreatus spawn in bottles. Bottles have several disadvantages: unsterilized lids, large weights, undetectable bacterial contaminations, and difficulties in removing the rest of mycelia around the edge of the bottle. Farmers rarely use plastic bags due to unfamiliarity. Therefore, in this study, we investigate growth of P. ostreatus spawn in heat-tolerant plastic bags using three different substrates (corn, rice, and a mixture of corn and rice). The physiological and morphological properties of mycelia growth on substrates were assessed, including growth rate, type of mycelium, and colony formation. Grain moisture is an important factor to get a successful spawn. We estimated the suitable water content of corn and rice in plastic bags was 50% and 27%, respectively. Inoculation of inoculum from potato dextrose agar (PDA) into grains was called first-generation spawn (G1). The corn medium showed the fastest growth (2 weeks), followed by the mixture of corn and rice (4 weeks), followed by the rice medium (6 weeks). Inoculation of G1 into another grain was called second-generation spawn (G2). Inoculation of G1 into G2 from corn to corn mycelia grew for 1 week, from the mixture of corn and rice on the same substrate for 2 weeks, and the slowest growth was in mycelium from rice to rice substrate for 4 weeks. An application of spawn to mushroom cultivation showed that farmers are able to expand G2 into 30-50 of sawdust fruiting body substrate. From the three types of spawn, farmers reported that they obtained good mushroom yield when the mixture of corn and rice was used as spawn. This study may be useful for new methods to generate spawn.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125801743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.010
F. Hidayat, R. Farrasati, I. Pradiko, E. Listia, M. Syarovy, S. Rahutomo, Winarna
Basal stem rot (BSR) disease is caused by Ganoderma boninense ; it has become a major disease in oil palm plantations over the years and causes a significant yield drop in oil palm plantations, especially in Sumatera. Nowadays, the incidence level of BSR across Sumatera has reached 39% and is predicted to keep increasing and threatening the sustainability of oil palm plantations between 2050 and 2100. Some researchers believe that Ganoderma is dominant due to the unbalance of the microbial community in the soil ecosystem. This study aims to discover the bacterial community structure in the soil under Ganoderma boninense infection in oil palm plantations. The study was conducted by comparing the soil infected by Ganoderma boninense (G+) and the healthy soil (G) through the next-generation sequencing (NGS) by Illumina MiSeq. The study shows that the total bacteria of the healthy soil (G) was 177 times higher than the endemic soil with a total copy number 1.32x10 8 and 7.44x10 5 , respectively. Acidobacteria was the dominant phyla in the healthy soil (G), followed by Proteobacteria, and their relative abundance are 31.45% and 29.19%, respectively. On the other hand, the relative abundance of Acidobacteria in the endemic soil (G+) was decreased to 18.73% while Proteobacteria was increased to 38.34%. However, the abundance of these phyla in the endemic soil (G+) is still lower than in the healthy soil (G). At the level species, the healthy soil (G) was more diverse than the endemic soil (G+). It shows that the endemic soil is more susceptible to Ganoderma boninense due to its dominance in the soil ecosystems. The 16S rRNA gene sequencing revealed that more than 60% of OTUs had <98% of similarity. It is indicated that some species, both in healthy soil (G) and endemic soil (G+), under oil palm plantations might be novel species.
{"title":"Preliminary Study on the Bacterial Community Structure of Ganoderma Soil Under Oil Palm Plantation","authors":"F. Hidayat, R. Farrasati, I. Pradiko, E. Listia, M. Syarovy, S. Rahutomo, Winarna","doi":"10.2991/absr.k.210810.010","DOIUrl":"https://doi.org/10.2991/absr.k.210810.010","url":null,"abstract":"Basal stem rot (BSR) disease is caused by Ganoderma boninense ; it has become a major disease in oil palm plantations over the years and causes a significant yield drop in oil palm plantations, especially in Sumatera. Nowadays, the incidence level of BSR across Sumatera has reached 39% and is predicted to keep increasing and threatening the sustainability of oil palm plantations between 2050 and 2100. Some researchers believe that Ganoderma is dominant due to the unbalance of the microbial community in the soil ecosystem. This study aims to discover the bacterial community structure in the soil under Ganoderma boninense infection in oil palm plantations. The study was conducted by comparing the soil infected by Ganoderma boninense (G+) and the healthy soil (G) through the next-generation sequencing (NGS) by Illumina MiSeq. The study shows that the total bacteria of the healthy soil (G) was 177 times higher than the endemic soil with a total copy number 1.32x10 8 and 7.44x10 5 , respectively. Acidobacteria was the dominant phyla in the healthy soil (G), followed by Proteobacteria, and their relative abundance are 31.45% and 29.19%, respectively. On the other hand, the relative abundance of Acidobacteria in the endemic soil (G+) was decreased to 18.73% while Proteobacteria was increased to 38.34%. However, the abundance of these phyla in the endemic soil (G+) is still lower than in the healthy soil (G). At the level species, the healthy soil (G) was more diverse than the endemic soil (G+). It shows that the endemic soil is more susceptible to Ganoderma boninense due to its dominance in the soil ecosystems. The 16S rRNA gene sequencing revealed that more than 60% of OTUs had <98% of similarity. It is indicated that some species, both in healthy soil (G) and endemic soil (G+), under oil palm plantations might be novel species.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130416484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.008
A. Larasati, D. Ryandini, O. Oedjijono, D. F. Kusharyati
{"title":"Optimization of Medium and Incubation Time in the Production of Antibacterial Compounds by Streptomyces sp. SA404","authors":"A. Larasati, D. Ryandini, O. Oedjijono, D. F. Kusharyati","doi":"10.2991/absr.k.210810.008","DOIUrl":"https://doi.org/10.2991/absr.k.210810.008","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"118 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123469697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.013
L. Prihastini, A. Ramelan, P. Setyono, Pranoto, A. Supriyanto
Large populations and final storage areas cause an increase in organic waste. Efforts are needed to improve waste decomposition. Mold is one of the microorganisms that can break down organic waste. This study aims to 1) isolate and identify molds from 8 banana bunches, 2) calculate mold populations. Banana bunches were taken from Madiun Regency. Isolation and mold conversion were carried out at the Biology Laboratory of the Department of Microbiology, Faculty of Science and Technology, Airlangga University, Surabaya. Isolation was carried out by making suspension of 8 banana bunches in which each suspension was inoculated on a petri dish with medium Potato Extract Agar. Subsequently it was incubated at 30°C for 3-7 days. After that the mold colonies were observed macroscopically and microcosically. After observing the mold colonies, identification and calculation of the population of each species were carried out using the Total Plate Count (TPC). From the results of the study, it was found 10 species of fungal, Aspergillus niger,Aspergillus penicilloides, Aspergillus oryzae, Rhizopus nigricans, Rhizopus oryzae, Mucor piriformis, Fucarium chlamydosporium, Penicillium citrinum, Penicillium citrinum, Penicillium chrysogenum, Penicillium expantium. The TPC of each species were 6.2 x 10 7 CFU/ml, 5.4 x 10 7 CFU/ml, 4.9 x 10 7 CFU/ml, 4.2 x 10 7 CFU/ml, 3.6 x 10 7 CFU/ml, 4.1x 10 7 CFU/ml, 4.1 x 10 7 CFU/ml, 3.3 x 10 7 CFU/ml, 3.8 x 10 7 CFU/ml, 4.1 x 10 7 CFU/ml. Insulated molds have the potential as bioinoculant acceleration of decomposition organic waste due to rapid growth.
{"title":"Isolation and Identification of Mold in Banana Bunches and Their Potential as Bioinoculants to Accelerate Decomposition of Household Organic Waste","authors":"L. Prihastini, A. Ramelan, P. Setyono, Pranoto, A. Supriyanto","doi":"10.2991/absr.k.210810.013","DOIUrl":"https://doi.org/10.2991/absr.k.210810.013","url":null,"abstract":"Large populations and final storage areas cause an increase in organic waste. Efforts are needed to improve waste decomposition. Mold is one of the microorganisms that can break down organic waste. This study aims to 1) isolate and identify molds from 8 banana bunches, 2) calculate mold populations. Banana bunches were taken from Madiun Regency. Isolation and mold conversion were carried out at the Biology Laboratory of the Department of Microbiology, Faculty of Science and Technology, Airlangga University, Surabaya. Isolation was carried out by making suspension of 8 banana bunches in which each suspension was inoculated on a petri dish with medium Potato Extract Agar. Subsequently it was incubated at 30°C for 3-7 days. After that the mold colonies were observed macroscopically and microcosically. After observing the mold colonies, identification and calculation of the population of each species were carried out using the Total Plate Count (TPC). From the results of the study, it was found 10 species of fungal, Aspergillus niger,Aspergillus penicilloides, Aspergillus oryzae, Rhizopus nigricans, Rhizopus oryzae, Mucor piriformis, Fucarium chlamydosporium, Penicillium citrinum, Penicillium citrinum, Penicillium chrysogenum, Penicillium expantium. The TPC of each species were 6.2 x 10 7 CFU/ml, 5.4 x 10 7 CFU/ml, 4.9 x 10 7 CFU/ml, 4.2 x 10 7 CFU/ml, 3.6 x 10 7 CFU/ml, 4.1x 10 7 CFU/ml, 4.1 x 10 7 CFU/ml, 3.3 x 10 7 CFU/ml, 3.8 x 10 7 CFU/ml, 4.1 x 10 7 CFU/ml. Insulated molds have the potential as bioinoculant acceleration of decomposition organic waste due to rapid growth.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127111099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.021
V. A. Cahya, C. Hanim, L. M. Yusiati
The purpose of this study was to characterize bacteriocins produced by Bacillus subtilis 11A isolated from Indonesian native chicken’s ileum . Characterization of bacteriocins included antimicrobial activity, the stability to temperature, pH, and storage time. Antimicrobial activity was tested against Escherichia coli FNCC 0091 , Salmonella typhimurium FNCC 0134, and Staphylococcus aureus FNCC 0143. Stability to temperature was tested to 4, 30, 70, 80, 90, 100 o C for an hour and 121 o C for 15 min. Stability to pH was tested to pH 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 for an hour. Stability to storage was tested for 7, 14, 21, and 28 days at refrigerator (4 o C) and room temperature (30 o C). The result showed that bacteriocins produced by Bacillus subtilis 11A were more effective against pathogens Gram-negative ( Escherichia coli and Salmonella typhimurium ) than Gram-positive ( Staphylococcus aureus ) (P<0.05). The bacteriocins produced by Bacillus subtilis 11A were heat until 90 o C, stable at pH 6–10 and stored at refrigerator up to 28 days (P<0.05). This study suggested that this bacteriocins might become potential candidate for use as biodegradable natural fed and alternative antimicrobial agents to solve the increasing trends of problems of antibiotic resistance
{"title":"Characterization of Bacteriocin Produced by Bacillus subtilis 11A Isolated from the Gastrointestinal Tract of Indonesian Native Chicken (Gallus domesticus)","authors":"V. A. Cahya, C. Hanim, L. M. Yusiati","doi":"10.2991/absr.k.210810.021","DOIUrl":"https://doi.org/10.2991/absr.k.210810.021","url":null,"abstract":"The purpose of this study was to characterize bacteriocins produced by Bacillus subtilis 11A isolated from Indonesian native chicken’s ileum . Characterization of bacteriocins included antimicrobial activity, the stability to temperature, pH, and storage time. Antimicrobial activity was tested against Escherichia coli FNCC 0091 , Salmonella typhimurium FNCC 0134, and Staphylococcus aureus FNCC 0143. Stability to temperature was tested to 4, 30, 70, 80, 90, 100 o C for an hour and 121 o C for 15 min. Stability to pH was tested to pH 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12 for an hour. Stability to storage was tested for 7, 14, 21, and 28 days at refrigerator (4 o C) and room temperature (30 o C). The result showed that bacteriocins produced by Bacillus subtilis 11A were more effective against pathogens Gram-negative ( Escherichia coli and Salmonella typhimurium ) than Gram-positive ( Staphylococcus aureus ) (P<0.05). The bacteriocins produced by Bacillus subtilis 11A were heat until 90 o C, stable at pH 6–10 and stored at refrigerator up to 28 days (P<0.05). This study suggested that this bacteriocins might become potential candidate for use as biodegradable natural fed and alternative antimicrobial agents to solve the increasing trends of problems of antibiotic resistance","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129390631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.020
T. Tallei, Fatimawali, A. M. Sumual, M. A. Gani, G. A. Pollo, A. A. Adam, J. Pelealu
Nowadays, the search for potential probiotics from lactic acid bacteria continues to develop exclusively for pharmaceutical applications. This study aimed at evaluating the potency of lactic acid bacteria (LAB) isolated from fermented brine of romaine lettuce as next-generation probiotics. The study began with romaine lettuce fermentation in a 10% salt solution for four days at room temperature in the dark. The LAB from the fermentation liquid were grown on MRS agar supplemented with CaCo 3 then purified. Purified colonies were identified using Gram-staining, catalase test, and 16S rRNA gene marker, and tested for their ability to be developed as the next-generation probiotics which included the following criteria: antibacterial activity, cholesterol assimilation, and their survival at pH3. The cholesterol-lowering ability was evaluated by incubating the bacteria in MRS agar supplemented with 0.5% taurodeoxycholic acid (TDCA). Well diffusion method was used to assess the antibacterial activity. Their ability to withstand acid environment at pH 3 was also evaluated. This study showed that all isolates (AS1, AS2, AS3, and AS4) survived at pH 3 for 2 hours and grew until the fifth day. Isolates AS2, AS3, and AS4 inhibited the growth of Staphylococcus aureus, while AS1, AS3, and AS4 inhibited the growth of Escherihia coli. All isolates had the ability to lower cholesterol. Isolate AS3 was molecularly identified as Enterococcus faecium. This isolate was chosen to be identified as it showed the best characteristics among all the isolates tested. We concluded that AS3 can be further developed as next-generation probiotic .
{"title":"Potential Next-Generation Probiotics Isolated from Romaine Lettuce (Lactuca sativa L. var. longifolia) Fermented Brine","authors":"T. Tallei, Fatimawali, A. M. Sumual, M. A. Gani, G. A. Pollo, A. A. Adam, J. Pelealu","doi":"10.2991/absr.k.210810.020","DOIUrl":"https://doi.org/10.2991/absr.k.210810.020","url":null,"abstract":"Nowadays, the search for potential probiotics from lactic acid bacteria continues to develop exclusively for pharmaceutical applications. This study aimed at evaluating the potency of lactic acid bacteria (LAB) isolated from fermented brine of romaine lettuce as next-generation probiotics. The study began with romaine lettuce fermentation in a 10% salt solution for four days at room temperature in the dark. The LAB from the fermentation liquid were grown on MRS agar supplemented with CaCo 3 then purified. Purified colonies were identified using Gram-staining, catalase test, and 16S rRNA gene marker, and tested for their ability to be developed as the next-generation probiotics which included the following criteria: antibacterial activity, cholesterol assimilation, and their survival at pH3. The cholesterol-lowering ability was evaluated by incubating the bacteria in MRS agar supplemented with 0.5% taurodeoxycholic acid (TDCA). Well diffusion method was used to assess the antibacterial activity. Their ability to withstand acid environment at pH 3 was also evaluated. This study showed that all isolates (AS1, AS2, AS3, and AS4) survived at pH 3 for 2 hours and grew until the fifth day. Isolates AS2, AS3, and AS4 inhibited the growth of Staphylococcus aureus, while AS1, AS3, and AS4 inhibited the growth of Escherihia coli. All isolates had the ability to lower cholesterol. Isolate AS3 was molecularly identified as Enterococcus faecium. This isolate was chosen to be identified as it showed the best characteristics among all the isolates tested. We concluded that AS3 can be further developed as next-generation probiotic .","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130673434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.028
NR Mubarik, H. Surya, Suryani
{"title":"Characterization of Crude Amylase Activity from Bacteriocin-Producing Lactobacilli","authors":"NR Mubarik, H. Surya, Suryani","doi":"10.2991/absr.k.210810.028","DOIUrl":"https://doi.org/10.2991/absr.k.210810.028","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125580140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.012
J. C. Ajiguna, V. C. Prakasita, T. Nahak, C. R. Tabbu, C. Santosa, A. Wahyuni
Feed is the main requirement for broilers farms. However, continuous use of AGP can cause antibiotic resistance in poultry and humans. The effectiveness of feed additives in preventing some disease agents has not been much scrutinized. Among the diseases that often arise is Salmonellosis. This study aims to inspect the role of synbiotics consisting of prebiotics and Saccharomyces sp. and Lactobacillus sp. as the probiotics (commercial product) on the performance and blood values of broilers challenged with Salmonella enteritidis. A total of 36 Cobb-strain DOCs were divided randomly into three groups of 12 with different diets. Group I was given broiler comercial feed, Group II was given comercial feed + AGP (enramycin dosage 250 g/ton), and Group III was given broiler comercial feed + Synbiotics (dosage 100 g/100 kg). Each of the three groups is then further divided into two groups of six, one of which is challenged with Salmonella enteritidis on day 22 (1 x 10 9 CFU/mL) for peroral. Vaccines were then given to all groups: ND + IB on day 7, IBD on day 14, and ND booster on day 18. Blood was collected on day 21 and 35, and body weights were taken every week until week 5. The results of this study showed slight differences in the weight gain amongs the groups, with Group III and group II gaining slightly more weights than Group I. The groups that were challenged with Salmonella enteritidis gained less weight compared to the groups that were not challenged in all groups. No significant difference in blood values was found among the groups. Group II and Group III showed slighty better blood values compared to Grup I. It is concuded, then, that the use of synbiotics (commercial product) as feed additives can replace antibiotic growth promotor (AGP) because the results are almost the same.
{"title":"The Role of Synbiotics (Commercial Product) as a Substitute for Antibiotic Growth Promotor (AGP) in the Performance and Blood Values of Cobb-strain Broilers Challenged with Salmonella enteritidis","authors":"J. C. Ajiguna, V. C. Prakasita, T. Nahak, C. R. Tabbu, C. Santosa, A. Wahyuni","doi":"10.2991/absr.k.210810.012","DOIUrl":"https://doi.org/10.2991/absr.k.210810.012","url":null,"abstract":"Feed is the main requirement for broilers farms. However, continuous use of AGP can cause antibiotic resistance in poultry and humans. The effectiveness of feed additives in preventing some disease agents has not been much scrutinized. Among the diseases that often arise is Salmonellosis. This study aims to inspect the role of synbiotics consisting of prebiotics and Saccharomyces sp. and Lactobacillus sp. as the probiotics (commercial product) on the performance and blood values of broilers challenged with Salmonella enteritidis. A total of 36 Cobb-strain DOCs were divided randomly into three groups of 12 with different diets. Group I was given broiler comercial feed, Group II was given comercial feed + AGP (enramycin dosage 250 g/ton), and Group III was given broiler comercial feed + Synbiotics (dosage 100 g/100 kg). Each of the three groups is then further divided into two groups of six, one of which is challenged with Salmonella enteritidis on day 22 (1 x 10 9 CFU/mL) for peroral. Vaccines were then given to all groups: ND + IB on day 7, IBD on day 14, and ND booster on day 18. Blood was collected on day 21 and 35, and body weights were taken every week until week 5. The results of this study showed slight differences in the weight gain amongs the groups, with Group III and group II gaining slightly more weights than Group I. The groups that were challenged with Salmonella enteritidis gained less weight compared to the groups that were not challenged in all groups. No significant difference in blood values was found among the groups. Group II and Group III showed slighty better blood values compared to Grup I. It is concuded, then, that the use of synbiotics (commercial product) as feed additives can replace antibiotic growth promotor (AGP) because the results are almost the same.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124971254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.026
Hasma, E. Abustam, R. Malaka, Mishiel Said, ZD Zainuddin
Goat skin is rich in protein compounds such as collagen, but it is highly bound to calcium minerals which have the potential to be processed into gelatin, so that for the denaturation process of skin proteins, fermented using L. plantarum . Curing stage on goat skin using chemical acid has done a lot, but using biologycal acid with Lactic Acid Bacteria (LAB) Lactobacillus plantarum is still very rare. The presence of lactic acid levels produced by BAL L. plantarum on MRS-Broth media that has been added to goat skin ensures that L. plantarum BAL bacteria can grow well. This research is the initial information to determine the ability of L. plantarum bacteria to grow on goat skin with indicators of bacterial population, lactic acid production, pH and total dissolved protein with different goat ages and fermentation times. This study uses a completely randomized design (CRD) factorial pattern 3 x 3 by treating the age factor of goats (1 year, 2 years and 3 years) and the fermentation time factor (24 hours, 48 hours and 72 hours). L. plantarum bacteria are isolated from milkfish, male goat skin Ettawah Breeds (PE) obtained from the same farm. Each treatment was repeated 4 times. The results showed that the bacterial population experienced rapid growth and a significant increase in the fermentation duration of 24 hours, 48 hours to 72 hours, the number of cells ranged from 8.50±0.45 log 10 CFU/mL, 8.81±0.41 log 10 CFU/mL. 9.34±0.70 log 10 CFU/mL. From this result, the duration of fermentation is 72 hours at most. Likewise, lactic acid production experienced a significant increase in the 24-hour fermentation duration to 72 hours starting from 1.03±0.17% -1.05± 0.04%. pH at 1 year of age 5.44 ± 0.37 tended to be acidic compared to 2 years and 3 years (5.60±0.16 and 5.60±0.26) which showed the age of 1 year was the highest acidity. Total dissolved protein showed a significant effect on the age of the goat and the duration of fermentation, the longer the fermentation (72 hours), the more dissolved protein (8.80% g/mL ±0.66% g/mL), the lower the age of the goat, the more goat protein dissolved high (1 year) (8.57% ±0.97% g/mL). The most effective growth ability of L. plantarum was obtained at 72 hours fermentation and 1 year old goats showed better quality than skin 1 and 2 years to be used as curing material in gelatin making.
{"title":"The Ability of Lactobacillus plantarum Bacteria to Denaturate Goat Skin in Producing Gelatin with Different Goat Ages and Fermentation Times","authors":"Hasma, E. Abustam, R. Malaka, Mishiel Said, ZD Zainuddin","doi":"10.2991/absr.k.210810.026","DOIUrl":"https://doi.org/10.2991/absr.k.210810.026","url":null,"abstract":"Goat skin is rich in protein compounds such as collagen, but it is highly bound to calcium minerals which have the potential to be processed into gelatin, so that for the denaturation process of skin proteins, fermented using L. plantarum . Curing stage on goat skin using chemical acid has done a lot, but using biologycal acid with Lactic Acid Bacteria (LAB) Lactobacillus plantarum is still very rare. The presence of lactic acid levels produced by BAL L. plantarum on MRS-Broth media that has been added to goat skin ensures that L. plantarum BAL bacteria can grow well. This research is the initial information to determine the ability of L. plantarum bacteria to grow on goat skin with indicators of bacterial population, lactic acid production, pH and total dissolved protein with different goat ages and fermentation times. This study uses a completely randomized design (CRD) factorial pattern 3 x 3 by treating the age factor of goats (1 year, 2 years and 3 years) and the fermentation time factor (24 hours, 48 hours and 72 hours). L. plantarum bacteria are isolated from milkfish, male goat skin Ettawah Breeds (PE) obtained from the same farm. Each treatment was repeated 4 times. The results showed that the bacterial population experienced rapid growth and a significant increase in the fermentation duration of 24 hours, 48 hours to 72 hours, the number of cells ranged from 8.50±0.45 log 10 CFU/mL, 8.81±0.41 log 10 CFU/mL. 9.34±0.70 log 10 CFU/mL. From this result, the duration of fermentation is 72 hours at most. Likewise, lactic acid production experienced a significant increase in the 24-hour fermentation duration to 72 hours starting from 1.03±0.17% -1.05± 0.04%. pH at 1 year of age 5.44 ± 0.37 tended to be acidic compared to 2 years and 3 years (5.60±0.16 and 5.60±0.26) which showed the age of 1 year was the highest acidity. Total dissolved protein showed a significant effect on the age of the goat and the duration of fermentation, the longer the fermentation (72 hours), the more dissolved protein (8.80% g/mL ±0.66% g/mL), the lower the age of the goat, the more goat protein dissolved high (1 year) (8.57% ±0.97% g/mL). The most effective growth ability of L. plantarum was obtained at 72 hours fermentation and 1 year old goats showed better quality than skin 1 and 2 years to be used as curing material in gelatin making.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123584550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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