Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.034
S. Sumarsih, S. Hadi, Fatimah, SL Bayasud
This research aims to study the effect of vegetable oils in the growth of of Lactococcus garvieae isolated from palm oil-contaminated soil and its active lipase production. The bacteria was grown in medium containing minerals and vegetable oils (virgin olive oil, virgin coconut oil, mustard oil and sunflower oil) as sole carbon sources. The bacteria growth was measured as optical density at =600 nm. The lipase production was determined as lipase activity of crude extract toward ρ-nitrophenyl palmitate as a substrate. The results showed that the vegetable oils used provided the good growth and lipase production of L.garvieae. Even though, olive oil had a great inductive effect on bacterial productivity. The addition of 1% olive oil improved the lipase activity by 135% compared to without olive oil. Crude extract with lipase activity of 56.790 66.571 U/mL produced by L. garvieae when cultivated in medium containing 1 5% olive oil for 20 hours. Electrophoregram of SDS PAGE and zymographic analysis showed that there were 2 types of lipases produced by L. garvieae, as indicated by the presence of protein bands around 85 and 45 kDa.
{"title":"Effect of Vegetable Oils in Growth of Lactococcus garvieae Isolated from Palm Oil-contaminated Soil and Its Lipase Production","authors":"S. Sumarsih, S. Hadi, Fatimah, SL Bayasud","doi":"10.2991/absr.k.210810.034","DOIUrl":"https://doi.org/10.2991/absr.k.210810.034","url":null,"abstract":"This research aims to study the effect of vegetable oils in the growth of of Lactococcus garvieae isolated from palm oil-contaminated soil and its active lipase production. The bacteria was grown in medium containing minerals and vegetable oils (virgin olive oil, virgin coconut oil, mustard oil and sunflower oil) as sole carbon sources. The bacteria growth was measured as optical density at =600 nm. The lipase production was determined as lipase activity of crude extract toward ρ-nitrophenyl palmitate as a substrate. The results showed that the vegetable oils used provided the good growth and lipase production of L.garvieae. Even though, olive oil had a great inductive effect on bacterial productivity. The addition of 1% olive oil improved the lipase activity by 135% compared to without olive oil. Crude extract with lipase activity of 56.790 66.571 U/mL produced by L. garvieae when cultivated in medium containing 1 5% olive oil for 20 hours. Electrophoregram of SDS PAGE and zymographic analysis showed that there were 2 types of lipases produced by L. garvieae, as indicated by the presence of protein bands around 85 and 45 kDa.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114358996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.030
Pujiati, M. W. Ardhi, E. Muktiani, N. K. Dewi, N. Jadid, E. Prasetyo
This study aims to determine the effect of substrate types on the crude activity of cellulase enzymes from local agricultural waste such as peanut shells, coconut fibers, brans, and teak leaves with the variations of incubation times (day 1, 3, 5, and 7) produced by Trichoderma viride . Enzyme activity was measured based on the amount of reducing sugar that produced by the DNS (Dinitrosalycilic Acid) method on a wavelength of 540 nm spectrophotometer. The results showed that the best substrate for cellulase production on coconut fibers substrate with an incubation period of 7 days which had the highest enzyme activity value of 1.340 U / ml. Coconut fibers contain the highest cellulose content compared to the other substrates. The lowest activity was shown by coconut fibers substrate at day one incubation time of 0.660 U / ml with the reducing sugar content of 0.594 mg/ml and protein content of 0.147 mg/ml. The complexity of the chemical composition of coconut fibers caused the longer time to degrade cellulose into glucose at day one than other three substrates.
本研究的目的是确定底物类型对当地农业废弃物(如花生壳、椰子纤维、麸皮和柚木叶)纤维素酶粗活性的影响，以及由绿色木霉产生的培养时间(第1、3、5和7天)的变化。用DNS(二硝基水杨酸)法在540 nm波长分光光度计上测定酶活性。结果表明，产纤维素酶的最佳底物为椰子纤维，培养7 d，酶活值最高，为1.340 U / ml，椰子纤维纤维素含量最高。椰子纤维底物在第1 d孵育时活性最低，为0.660 U /ml，还原糖含量为0.594 mg/ml，蛋白质含量为0.147 mg/ml。椰子纤维化学成分的复杂性导致纤维素在第一天降解为葡萄糖的时间比其他三种底物要长。
{"title":"The Effect of Incubation Time on Various Type of Local Agricultural Waste in Madiun, Indonesia to Produce Cellulases Using Trichoderma viride","authors":"Pujiati, M. W. Ardhi, E. Muktiani, N. K. Dewi, N. Jadid, E. Prasetyo","doi":"10.2991/absr.k.210810.030","DOIUrl":"https://doi.org/10.2991/absr.k.210810.030","url":null,"abstract":"This study aims to determine the effect of substrate types on the crude activity of cellulase enzymes from local agricultural waste such as peanut shells, coconut fibers, brans, and teak leaves with the variations of incubation times (day 1, 3, 5, and 7) produced by Trichoderma viride . Enzyme activity was measured based on the amount of reducing sugar that produced by the DNS (Dinitrosalycilic Acid) method on a wavelength of 540 nm spectrophotometer. The results showed that the best substrate for cellulase production on coconut fibers substrate with an incubation period of 7 days which had the highest enzyme activity value of 1.340 U / ml. Coconut fibers contain the highest cellulose content compared to the other substrates. The lowest activity was shown by coconut fibers substrate at day one incubation time of 0.660 U / ml with the reducing sugar content of 0.594 mg/ml and protein content of 0.147 mg/ml. The complexity of the chemical composition of coconut fibers caused the longer time to degrade cellulose into glucose at day one than other three substrates.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129694688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.016
N. Istifadah, L. Herawati
{"title":"The Potential of Microbes Isolated from Spent Substrate of Shiitake and Oyster Mushrooms to Induce Resistance Against Early Blight Disease in Tomatoes","authors":"N. Istifadah, L. Herawati","doi":"10.2991/absr.k.210810.016","DOIUrl":"https://doi.org/10.2991/absr.k.210810.016","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124964581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.027
I. Kresnawaty, D. Santoso, S. Hudiyono
Palm oil has a substantial contribution to national economy, as well as local economy development. At present the productivity of Indonesian oil plam plantation, especially small holderd is relatively low. Efforts to increase productivity can be done through biochemicals approach in this case enzymes that responsible for oil production. One of the bottle neck for oil production is the step converting acetyl co-A to malonyl co-A by acetyl co-A carboxylase (ACCs). ACCs in human is known to be activated with certain amino acid and organic acids, and divalen metals. For this reason this study was conducted to separate and study the activation of ACCs by those substances. Activity of ACCs was measured developed using HPLC method. Oil palm ACCs had 50 kDa of Biotin Carboxylase (BC) subunit dan 31 kDa of Carboxyl Transferase (CT) subunit. Separation of crude extract ACCs using dialysis and sephadex G-25 column chromatography showed the higher activity of enzyme. Addition of activator in the form of citric acid, Mg 2+ and glutamic acid could stimulate activity of the enzyme.
{"title":"Isolation and Activation of Fresh Fruit Bunch Oil Palm Acetyl Co-A Carboxylase","authors":"I. Kresnawaty, D. Santoso, S. Hudiyono","doi":"10.2991/absr.k.210810.027","DOIUrl":"https://doi.org/10.2991/absr.k.210810.027","url":null,"abstract":"Palm oil has a substantial contribution to national economy, as well as local economy development. At present the productivity of Indonesian oil plam plantation, especially small holderd is relatively low. Efforts to increase productivity can be done through biochemicals approach in this case enzymes that responsible for oil production. One of the bottle neck for oil production is the step converting acetyl co-A to malonyl co-A by acetyl co-A carboxylase (ACCs). ACCs in human is known to be activated with certain amino acid and organic acids, and divalen metals. For this reason this study was conducted to separate and study the activation of ACCs by those substances. Activity of ACCs was measured developed using HPLC method. Oil palm ACCs had 50 kDa of Biotin Carboxylase (BC) subunit dan 31 kDa of Carboxyl Transferase (CT) subunit. Separation of crude extract ACCs using dialysis and sephadex G-25 column chromatography showed the higher activity of enzyme. Addition of activator in the form of citric acid, Mg 2+ and glutamic acid could stimulate activity of the enzyme.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117021908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.006
C. Lombogia, M. Tulung, J. Posangi, T. Tallei
Honeybees are the world's most important pollinator of food crops. Besides, they also produce honey, which is very beneficial for human health. Therefore the health of honeybees is essential to note. One aspect that contributes to the health of honeybees is the balance of the gut microbiota. Lactic acid bacteria (LAB) are known to give importance to intestinal health in their hosts. This study aimed to study gut-associated LAB in Apis nigrocincta (Smith), a species of a cavity-nesting honeybee endemic to Sulawesi and Mindanao islands. The method used to obtain information about the presence of lactic acid bacteria was metagenomic profiling of the honeybee gut microbiome by targeting the V3V4 region of 16S rRNA genes using the Illumina MiSeq. In total, 83,018 reads of the microbiome comprising 749 bacterial operational taxonomic units (OTUs). Some genera belong to lactic acid bacteria, among others are Bifidobacterium, Enterococcus, Fructobacillus, Lactobacillus, Lactococcus, Leuconostoc, and Vagococcus. These LAB may pose beneficial properties, which are essential not only for the honeybee itself, but also the food and health industries.
{"title":"Gut-associated Lactic Acid Bacteria (LAB) in Apis nigrocincta (Smith)","authors":"C. Lombogia, M. Tulung, J. Posangi, T. Tallei","doi":"10.2991/absr.k.210810.006","DOIUrl":"https://doi.org/10.2991/absr.k.210810.006","url":null,"abstract":"Honeybees are the world's most important pollinator of food crops. Besides, they also produce honey, which is very beneficial for human health. Therefore the health of honeybees is essential to note. One aspect that contributes to the health of honeybees is the balance of the gut microbiota. Lactic acid bacteria (LAB) are known to give importance to intestinal health in their hosts. This study aimed to study gut-associated LAB in Apis nigrocincta (Smith), a species of a cavity-nesting honeybee endemic to Sulawesi and Mindanao islands. The method used to obtain information about the presence of lactic acid bacteria was metagenomic profiling of the honeybee gut microbiome by targeting the V3V4 region of 16S rRNA genes using the Illumina MiSeq. In total, 83,018 reads of the microbiome comprising 749 bacterial operational taxonomic units (OTUs). Some genera belong to lactic acid bacteria, among others are Bifidobacterium, Enterococcus, Fructobacillus, Lactobacillus, Lactococcus, Leuconostoc, and Vagococcus. These LAB may pose beneficial properties, which are essential not only for the honeybee itself, but also the food and health industries.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125259236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.022
C. M. Santosa, N. P. Rukmi, F. B. Lestari, M. Wasissa, D. A. Dewananda, S. I. Salasia
{"title":"Susceptibility Test of Staphylococcus aureus Isolated from Cow Milk, Goat Milk, and Dairy Farm Workers Against Various Antibiotics","authors":"C. M. Santosa, N. P. Rukmi, F. B. Lestari, M. Wasissa, D. A. Dewananda, S. I. Salasia","doi":"10.2991/absr.k.210810.022","DOIUrl":"https://doi.org/10.2991/absr.k.210810.022","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115250248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.005
C. Anam, E. Chasanah, B. P. Perdhana, ND Fajarningsih, N. Yusro, D. Praseptiangga, A. Yunus
Lectins or carbohydrate-binding proteins, are widely distributed in nature and also good candidates in such prospecting of algae. It may have been considered that binding specificity of lectins to some carbohydrates provokes to produce many unique biological activities, including cell agglutination, mitogenic activity, antibacterial activity and antitumor activity. The aim of this study was to determine the antibacterial effect of crude lectins fractions from red macro algae collected from the southern coast of Java island, Gunung Kidul Regency, Yogyakarta Indonesia. The.Crude lectin fraction showed no antibacterial activity against gram-negative bacteria because most of bacteria cells surface contain of mannan glycoprotein and the sample has no specificity to bind this glycoprotein type. Crude lectin fraction tested also have no antibacterial effect aginst gram positife bacteria because only binding specifically to N-glycan specifically as well as O-glycan.
{"title":"Antibacterial Effects of Crude Lectin Fraction Bioactive Compound of Red Macroalgae P. palmata and H. glandiforme from Southern Coast Java Island, Gunungkidul, Yogyakarta, Indonesia","authors":"C. Anam, E. Chasanah, B. P. Perdhana, ND Fajarningsih, N. Yusro, D. Praseptiangga, A. Yunus","doi":"10.2991/absr.k.210810.005","DOIUrl":"https://doi.org/10.2991/absr.k.210810.005","url":null,"abstract":"Lectins or carbohydrate-binding proteins, are widely distributed in nature and also good candidates in such prospecting of algae. It may have been considered that binding specificity of lectins to some carbohydrates provokes to produce many unique biological activities, including cell agglutination, mitogenic activity, antibacterial activity and antitumor activity. The aim of this study was to determine the antibacterial effect of crude lectins fractions from red macro algae collected from the southern coast of Java island, Gunung Kidul Regency, Yogyakarta Indonesia. The.Crude lectin fraction showed no antibacterial activity against gram-negative bacteria because most of bacteria cells surface contain of mannan glycoprotein and the sample has no specificity to bind this glycoprotein type. Crude lectin fraction tested also have no antibacterial effect aginst gram positife bacteria because only binding specifically to N-glycan specifically as well as O-glycan.","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131257166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.015
M. Canadianti, L. M. Yusiati, C. Hanim
{"title":"Effect of Nutmeg (Myristica fragrans Houtt) Leaves Tannin on in Vitro Fermentation Parameters and Methane Mitigation","authors":"M. Canadianti, L. M. Yusiati, C. Hanim","doi":"10.2991/absr.k.210810.015","DOIUrl":"https://doi.org/10.2991/absr.k.210810.015","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130253210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.038
M. Karyantina, S. Anggrahini, T. Utami, E. S. Rahayu
{"title":"The Use of Pediococcus halophillus FNCC-0032 and Pediococcus acidilactici F-11 to Suppress the Population of Salmonella – Shigella and Enterobacteriaceae in Jambal Roti (Fermented Fish)","authors":"M. Karyantina, S. Anggrahini, T. Utami, E. S. Rahayu","doi":"10.2991/absr.k.210810.038","DOIUrl":"https://doi.org/10.2991/absr.k.210810.038","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126129202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.2991/absr.k.210810.039
S. Kasmiyati, Ebe Kristiani, MM Herawati
{"title":"Antibacterial Activity and Secondary Metabolite Content of Polyploid Mutant of Artemisia cina Berg ex Poljakov","authors":"S. Kasmiyati, Ebe Kristiani, MM Herawati","doi":"10.2991/absr.k.210810.039","DOIUrl":"https://doi.org/10.2991/absr.k.210810.039","url":null,"abstract":"","PeriodicalId":445882,"journal":{"name":"Proceedings of the 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019)","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123657833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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