{"title":"Chemical Composition and UVA-Protecting Activity of Extracts from Ligustrum vulgare and Olea europaea Leaves","authors":"M. Czerwińska, K. Duszak, A. Parzonko, A. Kiss","doi":"10.1515/ABCSB-2016-0016","DOIUrl":"https://doi.org/10.1515/ABCSB-2016-0016","url":null,"abstract":"","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":"58 1","pages":"45-55"},"PeriodicalIF":1.3,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/ABCSB-2016-0016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67074068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Lantos, B. Jenes, L. Bóna, M. Cserhati, J. Pauk
{"title":"High frequency of doubled haploid plant production in spelt wheat","authors":"C. Lantos, B. Jenes, L. Bóna, M. Cserhati, J. Pauk","doi":"10.1515/ABCSB-2016-0014","DOIUrl":"https://doi.org/10.1515/ABCSB-2016-0014","url":null,"abstract":"","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":"58 1","pages":"107-112"},"PeriodicalIF":1.3,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/ABCSB-2016-0014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67074013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Prausová, L. Marečková, A. Kapler, Ľ. Majeský, T. Farkas, A. Indreica, L. Šafářová, Miloslav Kitner
{"title":"Adenophora liliifolia: condition of its populations in Central Europe","authors":"R. Prausová, L. Marečková, A. Kapler, Ľ. Majeský, T. Farkas, A. Indreica, L. Šafářová, Miloslav Kitner","doi":"10.1515/ABCSB-2016-0018","DOIUrl":"https://doi.org/10.1515/ABCSB-2016-0018","url":null,"abstract":"","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":"58 1","pages":"83-105"},"PeriodicalIF":1.3,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Paul, E. Cieślak, M. Ronikier, G. Migdałek, A. Słomka, J. Żabicka
{"title":"Low genetic diversity of declining Viola uliginosa (Violaceae) at its southern range limits in Poland","authors":"W. Paul, E. Cieślak, M. Ronikier, G. Migdałek, A. Słomka, J. Żabicka","doi":"10.1515/ABCSB-2016-0015","DOIUrl":"https://doi.org/10.1515/ABCSB-2016-0015","url":null,"abstract":"","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":"58 1","pages":"71-82"},"PeriodicalIF":1.3,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/ABCSB-2016-0015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67074053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Plants of the genus Bulbophyllum Thouars have some of the most intricate flowers and complex pollination mechanisms to be found amongst the Orchidaceae (van der Pijl and Dodson, 1969; van der Cingel, 2001). For an up-to-date, overall account of the genus, readers are referred to Pridgeon et al. (2014). Many of its members are pollinated by dipterans (van der Pijl and Dodson, 1969; van der Cingel, 2001) attracted by foodrewards and fragrance. However, to date, these food-rewards have been investigated for relatively few species, mostly Neotropical and African taxa, and have generally been found to be lipid-rich and produced by palisade-like epidermal cells of the
球叶属植物拥有兰科植物中最复杂的花朵和最复杂的授粉机制(van der Pijl and Dodson, 1969;范德辛格尔，2001)。对于最新的，属的整体帐户，读者可参考Pridgeon等人(2014)。它的许多成员是由双翅传粉者授粉的(van der Pijl和Dodson, 1969;van der Cingel, 2001)被美食和香味所吸引。然而，迄今为止，对这些食物奖励的研究相对较少，主要是新热带和非洲分类群，并且通常被发现富含脂质并由植物的栅栏状表皮细胞产生
{"title":"Evidence for the Dual Role of Floral Secretory Cells in Bulbophyllum","authors":"M. Stpiczyńska, K. L. Davies.","doi":"10.1515/ABCSB-2016-0013","DOIUrl":"https://doi.org/10.1515/ABCSB-2016-0013","url":null,"abstract":"Plants of the genus Bulbophyllum Thouars have some of the most intricate flowers and complex pollination mechanisms to be found amongst the Orchidaceae (van der Pijl and Dodson, 1969; van der Cingel, 2001). For an up-to-date, overall account of the genus, readers are referred to Pridgeon et al. (2014). Many of its members are pollinated by dipterans (van der Pijl and Dodson, 1969; van der Cingel, 2001) attracted by foodrewards and fragrance. However, to date, these food-rewards have been investigated for relatively few species, mostly Neotropical and African taxa, and have generally been found to be lipid-rich and produced by palisade-like epidermal cells of the","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":"58 1","pages":"57-69"},"PeriodicalIF":1.3,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Mizia, M. Cygan, M. Denysenko, D. Kwolek, A. Chramiec-Głąbik, A. Grabowska-Joachimiak, A. Joachimiak
{"title":"Development of a RAPD-Based Male-Specific Molecular Marker in Japanese Hop (Humulus japonicus Siebold & Zucc.)","authors":"P. Mizia, M. Cygan, M. Denysenko, D. Kwolek, A. Chramiec-Głąbik, A. Grabowska-Joachimiak, A. Joachimiak","doi":"10.1515/ABCSB-2016-0017","DOIUrl":"https://doi.org/10.1515/ABCSB-2016-0017","url":null,"abstract":"","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":"58 1","pages":"21-27"},"PeriodicalIF":1.3,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/ABCSB-2016-0017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67074080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rehmannia glutinosa hairy roots were used to evaluate the effect of methyl jasmonate (MeJa) and salicylic acid (SA) on increase of root biomass and production of iridoids (catalpol, harpagide) and phenylethanoids (verbascoside and isoverbascoside). The elicitors were added to 23-day-old culture separately at concentrations between 50 and 200 μM or in combinations at concentrations of 50 and 100 μM. Roots were harvested 72 h and 120 h after elicitation. The type of elicitor, its concentration and exposure time were found to strongly affect the content of each analyzed compound. A 72-hour treatment with 200 μM MeJa was the most effective in increase of verbascoside content (60.07 mg·DW-1 equivalent to 845.45 mg·L-1) and isoverbascoside (1.77 mg·DW-1 equivalent to 24.94 mg·L-1): these respective amounts were roughly 10and 6.4-fold higher than the control values (unelicited roots). Exposure to 150 μM MeJa provided optimal harpagide content after 72 hours (0.136 mg·DW-1; 7.5-fold increase compared to the control), and catalpol content after 120 hours (up to 2.145 mg·DW-1). The combination of MeJa and SA also resulted in higher levels of secondary metabolites compared to the control culture, although these levels were lower than those observed for MeJa alone at the optimal concentration and exposure time. SA alone was less efficient in enhancing metabolite production than MeJa.
{"title":"The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture","authors":"E. Piątczak, Ł. Kuźma, H. Wysokińska","doi":"10.1515/ABCSB-2016-0004","DOIUrl":"https://doi.org/10.1515/ABCSB-2016-0004","url":null,"abstract":"Rehmannia glutinosa hairy roots were used to evaluate the effect of methyl jasmonate (MeJa) and salicylic acid (SA) on increase of root biomass and production of iridoids (catalpol, harpagide) and phenylethanoids (verbascoside and isoverbascoside). The elicitors were added to 23-day-old culture separately at concentrations between 50 and 200 μM or in combinations at concentrations of 50 and 100 μM. Roots were harvested 72 h and 120 h after elicitation. The type of elicitor, its concentration and exposure time were found to strongly affect the content of each analyzed compound. A 72-hour treatment with 200 μM MeJa was the most effective in increase of verbascoside content (60.07 mg·DW-1 equivalent to 845.45 mg·L-1) and isoverbascoside (1.77 mg·DW-1 equivalent to 24.94 mg·L-1): these respective amounts were roughly 10and 6.4-fold higher than the control values (unelicited roots). Exposure to 150 μM MeJa provided optimal harpagide content after 72 hours (0.136 mg·DW-1; 7.5-fold increase compared to the control), and catalpol content after 120 hours (up to 2.145 mg·DW-1). The combination of MeJa and SA also resulted in higher levels of secondary metabolites compared to the control culture, although these levels were lower than those observed for MeJa alone at the optimal concentration and exposure time. SA alone was less efficient in enhancing metabolite production than MeJa.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":"58 1","pages":"57-65"},"PeriodicalIF":1.3,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/ABCSB-2016-0004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Sytykiewicz, M. Horbowicz, W. Wiczkowski, D. Koczkodaj, J. Mitrus, T. Sawicki, Julia Sławianowska, M. Szwed
{"title":"Methyl Jasmonate Elicitation Affects Expression of Genes Involved in Biosynthesis and Turnover of 2-Phenylethylamine in Maize Seedlings","authors":"H. Sytykiewicz, M. Horbowicz, W. Wiczkowski, D. Koczkodaj, J. Mitrus, T. Sawicki, Julia Sławianowska, M. Szwed","doi":"10.1515/ABCSB-2016-0005","DOIUrl":"https://doi.org/10.1515/ABCSB-2016-0005","url":null,"abstract":"","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":"58 1","pages":"67-80"},"PeriodicalIF":1.3,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/ABCSB-2016-0005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Lazaridou, C. Pankou, Ioannis Nikolaos Xynias, D. Roupakias
The present study was conducted to determine the effect of the D genome on embryoid induction and green plant regeneration in wheat anther culture and how it is influenced by low temperature and mannitol treatment. For this reason, the anther culture response of two Canadian bread wheat cultivars and their extracted tetraploids (AABB) was studied. As controls two cultivars well responding to anther-culture (i.e. cvs. Kavkaz/Cgn and Acheron) and a no-responding cultivar (cv. Vergina) were used. Approximately 3000 anthers of these cultivars were cultured and three pre-treatments were applied: cold pre-treatment for 7 and 18 days at 4oC, and 0.3M mannitol for seven days at 4oC. W14 and 190-2 were used as induction and regeneration media, respectively, and the basic MS medium as the rooting medium. No green plants were produced from the tetraploids, which supports the view that the D-genome chromosomes are necessary for androgenic response in wheat. Furthermore, the Canadian cultivars performed better after 18-day pre-treatment at 4oC. The extracted tetraploids produced fewer embryoids and performed better after seven days of cold pre-treatment. The controls well responding to anther culture performed better than the Canadian cultivars, although their best response was recorded after seven-day cold pre-treatment. Cultivar Vergina produced no green plants. The presence of mannitol influenced negatively both embryoid and green plant production. It was concluded that the D genome plays a crucial role in anther culture response of wheat and that this response is influenced by both the genotype and the duration of cold pre-treatment.
{"title":"Effect of D Genome on Wheat Anther Culture Response After Cold and Mannitol Pretreatment","authors":"T. Lazaridou, C. Pankou, Ioannis Nikolaos Xynias, D. Roupakias","doi":"10.1515/ABCSB-2016-0006","DOIUrl":"https://doi.org/10.1515/ABCSB-2016-0006","url":null,"abstract":"The present study was conducted to determine the effect of the D genome on embryoid induction and green plant regeneration in wheat anther culture and how it is influenced by low temperature and mannitol treatment. For this reason, the anther culture response of two Canadian bread wheat cultivars and their extracted tetraploids (AABB) was studied. As controls two cultivars well responding to anther-culture (i.e. cvs. Kavkaz/Cgn and Acheron) and a no-responding cultivar (cv. Vergina) were used. Approximately 3000 anthers of these cultivars were cultured and three pre-treatments were applied: cold pre-treatment for 7 and 18 days at 4oC, and 0.3M mannitol for seven days at 4oC. W14 and 190-2 were used as induction and regeneration media, respectively, and the basic MS medium as the rooting medium. No green plants were produced from the tetraploids, which supports the view that the D-genome chromosomes are necessary for androgenic response in wheat. Furthermore, the Canadian cultivars performed better after 18-day pre-treatment at 4oC. The extracted tetraploids produced fewer embryoids and performed better after seven days of cold pre-treatment. The controls well responding to anther culture performed better than the Canadian cultivars, although their best response was recorded after seven-day cold pre-treatment. Cultivar Vergina produced no green plants. The presence of mannitol influenced negatively both embryoid and green plant production. It was concluded that the D genome plays a crucial role in anther culture response of wheat and that this response is influenced by both the genotype and the duration of cold pre-treatment.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":"58 1","pages":"95-102"},"PeriodicalIF":1.3,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/ABCSB-2016-0006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The total soluble sugar content and antioxidant enzyme activities were studied for the first time during axillary shoot formation in Magnolia × ‘Spectrum’ in vitro in response to BAP (0.3 mg l -1 ), different levels of gibberellic acid (GA 3 ; 0.0, 0.1, 0.5, 1.0 mg l -1 ), sucrose (20 and 30 g l -1 ) and nitrogen salts (KNO 3 /NH 4 NO 3 ; 100/100% and 75/50% relative to MS medium). Among various GA 3 and sucrose/nitrogen salts ratios, the most effective axillary multiplication (5.9 shoots/explant) and leaf formation (25.7 leaves per multiplied clumps) were obtained after addition of GA 3 at 0.1 mg l -1 to a BAP medium containing 20 g l -1 sucrose and reduced levels of nitrogen salts (75% KNO 3 and 50% NH 4 NO 3 ). The addition of GA 3 to the BAP medium enhanced shoot formation by 36% and leaf formation by 27%. The highest shoot formation capacity of M. × ‘Spectrum’ in vitro coincided with enhanced levels of soluble sugar and peroxidase (POD) activity. Increasing GA 3 concentration from 0.1 to 1.0 mg l -1 in the above medium resulted in inhibition of shoot and leaf formation and a decrease in the soluble sugar content. The influence of GA 3 on the activities of catalase (CAT) and POD depended on its concentration and the levels of sucrose and nitrogen salts in the medium. The highest increase in CAT and POD activities, that coincided with the enhanced shoot formation capacity of M. × ‘Spectrum’ in vitro, was observed after addition of GA 3 to the medium containing high levels of sucrose and nitrogen salts. presence of different concentration of GA 3 , sucrose and nitrogen salts were studied.
首次研究了白玉兰腋芽形成过程中BAP (0.3 mg l -1)、不同浓度赤霉素(GA 3;0.0、0.1、0.5、1.0 mg l -1)、蔗糖(20和30 g l -1)和氮盐(kno3 / nh4 no3;相对于MS培养基100/100%和75/50%)。在不同的甘油三酯和蔗糖/氮盐配比下，甘油三酯在含有20 g l -1蔗糖和降低氮盐浓度(75% kno3和50% nh4 no3)的BAP培养基中添加0.1 mg l -1甘油三酯后，腋生增殖(5.9芽/外植体)和叶片形成(25.7叶/扩增簇)最有效。在BAP培养基中添加ga3使芽形成率提高36%，叶片形成率提高27%。M. x ' Spectrum '的体外芽形成能力最高，与可溶性糖和过氧化物酶(POD)活性的提高一致。在上述培养基中，将ga3浓度从0.1 mg l -1增加到1.0 mg l -1，可抑制茎和叶的形成，降低可溶性糖含量。GA - 3对过氧化氢酶(CAT)和POD活性的影响取决于其浓度和培养基中蔗糖和氮盐的水平。在高水平蔗糖和氮盐的培养基中添加GA 3后，M. x '谱'的CAT和POD活性的提高最高，与体外芽形成能力的增强一致。研究了不同浓度ga3、蔗糖和氮盐的存在。
{"title":"Morphological and biochemical responses to gibberellic acid in Magnolia × 'spectrum' in vitro.","authors":"A. Wojtania, E. Skrzypek, E. Gabryszewska","doi":"10.1515/ABCSB-2016-0010","DOIUrl":"https://doi.org/10.1515/ABCSB-2016-0010","url":null,"abstract":"The total soluble sugar content and antioxidant enzyme activities were studied for the first time during axillary shoot formation in Magnolia × ‘Spectrum’ in vitro in response to BAP (0.3 mg l -1 ), different levels of gibberellic acid (GA 3 ; 0.0, 0.1, 0.5, 1.0 mg l -1 ), sucrose (20 and 30 g l -1 ) and nitrogen salts (KNO 3 /NH 4 NO 3 ; 100/100% and 75/50% relative to MS medium). Among various GA 3 and sucrose/nitrogen salts ratios, the most effective axillary multiplication (5.9 shoots/explant) and leaf formation (25.7 leaves per multiplied clumps) were obtained after addition of GA 3 at 0.1 mg l -1 to a BAP medium containing 20 g l -1 sucrose and reduced levels of nitrogen salts (75% KNO 3 and 50% NH 4 NO 3 ). The addition of GA 3 to the BAP medium enhanced shoot formation by 36% and leaf formation by 27%. The highest shoot formation capacity of M. × ‘Spectrum’ in vitro coincided with enhanced levels of soluble sugar and peroxidase (POD) activity. Increasing GA 3 concentration from 0.1 to 1.0 mg l -1 in the above medium resulted in inhibition of shoot and leaf formation and a decrease in the soluble sugar content. The influence of GA 3 on the activities of catalase (CAT) and POD depended on its concentration and the levels of sucrose and nitrogen salts in the medium. The highest increase in CAT and POD activities, that coincided with the enhanced shoot formation capacity of M. × ‘Spectrum’ in vitro, was observed after addition of GA 3 to the medium containing high levels of sucrose and nitrogen salts. presence of different concentration of GA 3 , sucrose and nitrogen salts were studied.","PeriodicalId":45465,"journal":{"name":"Acta Biologica Cracoviensia Series Botanica","volume":"58 1","pages":"103-111"},"PeriodicalIF":1.3,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/ABCSB-2016-0010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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