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In vitro assessment of probiotic potential of selected bacteria isolated from pig faeces with potential application of odour reduction. 从猪粪便中分离的益生菌的体外评估及其在减臭方面的潜在应用。
IF 2.2 Q1 VETERINARY SCIENCES Pub Date : 2021-07-02 eCollection Date: 2021-01-01 DOI: 10.1080/23144599.2021.1936962
M Jahangir Alam, Mahfuzul Islam, Che-Ok Jeon, Ki-Choon Lee, Seon-Ho Kim, Chul-Ju Yang, M Enayet Kabir, Sang-Suk Lee

To evaluate the odour reduction potential of four different bacterial species such as Enterococcus faecium, Enterococcus faecalis, Acetobacter tropicalis, and Bacillus subtilis subsp. subtilis that were isolated from fresh faeces of pigs and identified based on16S rDNA gene sequence analyses. Faecal slurry in anaerobic salt medium with 1% soluble starch (which was served as control group) and the addition of four different isolated bacterial cultures (1.0 × 107CFU/mL), designated as M1, M2, M3, and M4, respectively, were incubated anaerobically for 12 and 24 h. Total gas production was increased with the incubation period (p < 0.05). M1 and M4 had decreased pattern (p < 0.05) of ammonia and hydrogen sulphide gas from 12 to 24 h. The lowest total volatile fatty acids (p < 0.05), highest lactate, and moderate butyrate concentration was observed in the M1 group at 24 h of incubation. Likewise, M1 group had the lowest total biogenic amine, histamine, ethylamine, putrescine, methylamine, and cadaverine compared to the other groups (p < 0.05) at 24 h of incubation. Overall results suggest that E. faecium can be used as a potent odour reducer in pigs production.

评价粪肠球菌、粪肠球菌、热带醋酸杆菌和枯草芽孢杆菌等四种不同细菌的减臭潜力。从猪新鲜粪便中分离得到的枯草杆菌,通过16s rDNA基因序列分析鉴定。将粪浆在含1%可溶性淀粉的厌氧盐培养基中(作为对照组),并添加4种不同的分离细菌培养物(1.0 × 107CFU/mL),分别命名为M1、M2、M3和M4,厌氧培养12 h和24 h。总产气量随着培养时间的延长而增加(p)。在12 ~ 24 h内,M1和M4的氨气和硫化氢气体含量呈下降趋势(p)。最低的总挥发性脂肪酸(p p E. faecium)可作为猪生产中有效的减臭剂。
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引用次数: 1
Optimization of Chitin Extraction, Physicochemical and Functional Properties of Chitosan Production from Shells of Karamote Shrimp Peneaus (Melicertus) Kerathurus in Western Greece 希腊卡拉莫特对虾壳甲壳素提取工艺优化、壳聚糖理化性质及功能特性研究
IF 2.2 Q1 VETERINARY SCIENCES Pub Date : 2021-06-30 DOI: 10.13188/2325-4645.1000052
This research aims to optimize by chemical methods the extraction of chitin and chitosan from shells of Karamote shrimp Peneaus (Melicertus) kerathurus . Shrimp waste can be used as source of high value compounds. Chitin is a major component of the exoskeleton of invertebrates and chemically is a linear polysaccharide of β (1→4) linked N-acetylglucosamine monomers. Chitosan is a deacetylated form of chitin having d-glucosamine repeating units linked by β (1-4) glycosidic bond. The extraction method uses different concentrations of sodium or potassium hydroxide in the deproteinization (protein separation) and deacetylation (remove acetyl groups) step and hydrochloric acid for demineralization (separation of calcium carbonate and calcium phosphate) to yield optimum output. Among all experiments, results of 1.0 N solution of HCl for demineralization, 2 N for deproteination and 12.5 N NaOH solutions for deacetylation at solid to solvent ratio of 1: 15, clearly demonstrate a significant yield of chitin and chitosan. The results obtained from this study show also that the solubility of chitosan in 1% acetic acid solution achieved up to 90%.
本研究旨在通过化学方法优化从卡拉对虾(Peneaus (Melicertus) kerathurus)壳中提取甲壳素和壳聚糖的工艺条件。虾废物可作为高价值化合物的来源。甲壳素是无脊椎动物外骨骼的主要成分,化学上是β(1→4)连接n -乙酰氨基葡萄糖单体的线性多糖。壳聚糖是甲壳素的去乙酰化形式,具有由β(1-4)糖苷键连接的d-葡萄糖胺重复单元。该提取方法采用不同浓度的氢氧化钠或氢氧化钾进行脱蛋白(蛋白质分离)和去乙酰化(去除乙酰基)步骤,用盐酸进行脱矿(碳酸钙和磷酸钙分离),以获得最佳产量。其中1.0 N盐酸脱矿、2 N脱蛋白和12.5 N NaOH脱乙酰,固液比为1:15时,甲壳素和壳聚糖的收率显著。结果表明,壳聚糖在1%醋酸溶液中的溶解度可达90%以上。
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引用次数: 1
A retrospective analysis of antimicrobial resistance in pathogenic Escherichia coli and Salmonella spp. isolates from poultry in Uganda. 乌干达家禽致病性大肠杆菌和沙门氏菌分离株抗微生物药物耐药性的回顾性分析。
IF 2.2 Q1 VETERINARY SCIENCES Pub Date : 2021-05-19 DOI: 10.1080/23144599.2021.1926056
Steven Kakooza, Adrian Muwonge, Esther Nabatta, Wilfred Eneku, Dickson Ndoboli, Eddie Wampande, Damian Munyiirwa, Edrine Kayaga, Maria Agnes Tumwebaze, Mathias Afayoa, Paul Ssajjakambwe, Dickson Stuart Tayebwa, Sayaka Tsuchida, Torahiko Okubo, Kazunari Ushida, Ken'ichi Sakurai, Francis Mutebi

There are increasing reports of antimicrobial treatment failures for bacterial diseases of poultry in Uganda. The paucity of data on antimicrobial resistance (AMR) of pathogenic bacteria in Uganda is a major setback to AMR control. This study investigated the occurrence of fowl typhoid, colibacillosis, and AMR in associated pathogens from 2012 to 2018. Laboratory records from the Central Diagnostic Laboratory (CDL), a National Veterinary Diagnostic Facility located at Makerere University, were reviewed. Archived isolates of the causative bacteria for the two diseases were also evaluated for AMR. The frequencies of the two disease conditions, their clinical and necropsy presentations and the demographic data of the diagnostic samples were summarized from the records. Archived bacterial isolates were revived before antimicrobial susceptibility testing. This was done on Mueller Hinton agar using the disk diffusion method, against 16 antimicrobials of medical and veterinary importance according to the Clinical Laboratory Standards Institute guidelines. A total of 697 poultry cases were presented for bacteriological investigations in the review period. Colibacillosis and salmonellosis had prevalence rates of 39.7% (277/697) and 16.2% (113/697), respectively. A total of 63 and 92 isolates of Escherichia coli and Salmonella spp., respectively, were archived but 43 (68.3%) E. coli and 47 (51.1%) Salmonella spp. isolates were recovered and evaluated for AMR. Multidrug resistance was more frequent in E. coli (38; 88.4%) than salmonellae (25; 53.2%), (p < 0.001). The high prevalence of colibacillosis, salmonellosis and the AMR of associated pathogens warrants immediate institution of appropriate disease control measures.

关于乌干达家禽细菌性疾病抗菌治疗失败的报告越来越多。乌干达致病菌抗菌素耐药性数据的缺乏是抗菌素耐药性控制的一个重大挫折。本研究调查了2012 - 2018年家禽伤寒、大肠杆菌病和AMR相关病原体的发生情况。审查了位于马凯雷雷大学的国家兽医诊断机构中央诊断实验室(CDL)的实验室记录。还对存档的两种疾病病原菌分离株进行了AMR评估。从记录中总结了两种疾病的频率、临床和尸检表现以及诊断样本的人口统计数据。存档的细菌分离株在抗菌药敏试验前恢复。根据临床实验室标准协会的指导方针,使用圆盘扩散法在Mueller Hinton琼脂上对16种具有医学和兽医重要性的抗菌剂进行了试验。报告期内共收到697例家禽病例进行细菌学调查。大肠杆菌病和沙门氏菌病患病率分别为39.7%(277/697)和16.2%(113/697)。检出大肠杆菌63株,沙门菌92株;检出大肠杆菌43株(68.3%),沙门菌47株(51.1%)。多药耐药在大肠杆菌中更为常见(38;88.4%)高于沙门氏菌(25;53.2%) (p
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引用次数: 10
Aprosopia/holoprosencephaly in a stillborn puppy: when the face predicts the brain. 死产小狗的无脸畸形/前脑全裂:当脸预示大脑时。
IF 2.2 Q1 VETERINARY SCIENCES Pub Date : 2021-03-26 DOI: 10.1080/23144599.2021.1897740
Clairton Marcolongo Pereira, Tayná B Silva, Laiz Zaché Roque, Bárbara Barros, Luiz Alexandre Moscon, Ana Lucia Schild, Claudio S L Barros, Leonardo Schüler-Faccini, Lavinia Schuler-Faccini

In a litter of three puppies, one was stillborn and had facial and brain defects. Fusion of the maxilla and mandible and absence of the face were observed. The forebrain (telencephalon and the diencephalon) was reduced in size and fused, and the telencephalic longitudinal fissure, olfactory bulbs, and optic nerves were absent (Figures 6 and 7). Lissencephaly was observed in the telencephalon and cerebellum. A diagnosis of aprosopia/holoprosencephaly was made.

在一窝三只小狗中,有一只胎死腹中,有面部和大脑缺陷。观察到上颌和下颌骨融合,面部缺失。前脑(端脑和间脑)缩小融合,端脑纵裂、嗅球和视神经缺失(图6和7)。在端脑和小脑中观察到无脑畸形。诊断为无前脑畸形/全前脑畸形。
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引用次数: 1
Arterial medial calcification (Mönckeberg's sclerosis) with chronic renal disease in a zoo-kept Southern tamandua (Tamandua tetradactyla). 动脉内侧钙化(Mönckeberg硬化)与慢性肾脏疾病在动物园饲养的南塔曼杜鹃(塔曼杜鹃四趾甲)。
IF 2.2 Q1 VETERINARY SCIENCES Pub Date : 2021-03-24 DOI: 10.1080/23144599.2021.1899408
Tohru Kimura, Kengo Inaka

Arterial medial calcification observed in animals is equivalent to Mönckeberg's sclerosis in human beings. This lesion is rarely reported in domestic animals. In addition, little information is available concerning the occurrence of arterial medial calcification in zoo animals. The aim of the current work was to report arterial medial calcification incidentally encountered in a Southern tamandua (Tamandua tetradactyla). This paper described the clinical findings, haematological and serum biochemical profiles, and histopathological results. Haematological examinations showed decreases in white blood cell counts, erythrocytic parameters and platelets. In serum biochemical examinations, both of the creatinine and blood urea nitrogen levels markedly increased in this study. Glucose concentrations dramatically declined from the normal levels to the critical conditions. There was electrolytic imbalance which was not accompanied by increases in calcium and inorganic phosphorus concentrations. Enzyme activities (ALP, AST, ALP, γ-GT, LDH and CK) remarkably increased just before the animal died. Histopathological examinations revealed that this marked and thickened calcification extended linearly around the circumference of the vessels. The calcified deposits were located entirely within the medial layer of the arterial wall. The lesions showed involvement of the internal elastic lamina with calcification. Severe calcification was observed in the glomerular capsules, indicating serious chronic kidney disease. We concluded that arterial medial calcification in the Southern tamandua arose with progressing chronic kidney failure.

在动物身上观察到的动脉内侧钙化相当于人类Mönckeberg的硬化。这种病变在家畜中很少报道。此外,关于动物园动物动脉内侧钙化的发生资料很少。目前的工作目的是报告偶然遇到的动脉内侧钙化在南塔曼杜亚(塔曼杜亚四趾)。本文描述了临床表现、血液学和血清生化特征以及组织病理学结果。血液学检查显示白细胞计数、红细胞参数和血小板减少。在血清生化检查中,肌酐和尿素氮水平均明显升高。葡萄糖浓度从正常水平急剧下降到临界状态。存在电解质失衡,但不伴有钙和无机磷浓度的增加。酶活性(ALP、AST、ALP、γ-GT、LDH和CK)在动物死亡前显著升高。组织病理学检查显示,这种明显的、增厚的钙化在血管周围呈线性延伸。钙化沉积物完全位于动脉壁内侧。病变表现为累及内部弹性板并伴有钙化。肾小球囊严重钙化,提示严重的慢性肾病。我们的结论是,南tamandua动脉内侧钙化是随着慢性肾衰竭的进展而出现的。
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引用次数: 3
Characterisation and Recognition by Immune Hosts of a Sheep Nematode Parasite Teladorsagia circumcincta Chitinase 一种绵羊线虫寄生环切端棘虫几丁质酶的鉴定及免疫宿主的识别
IF 2.2 Q1 VETERINARY SCIENCES Pub Date : 2021-01-01 DOI: 10.13188/2325-4645.1000055
AgResearch Ltd, Invi t ing Innova t ions
Characterisation and
描述和
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引用次数: 0
Behavioural Correlates of Neutering Male Dogs -a Question of Breed? 雄性绝育犬的行为相关性——品种问题?
IF 2.2 Q1 VETERINARY SCIENCES Pub Date : 2021-01-01 DOI: 10.13188/2325-4645.1000054
CA Kolkmeyer
Castration of dogs is often conducted as a preventive measure against diseases and undesirable behaviour. While female dogs are preferably neutered for medical reasons, owners of male dogs hope for an improvement in behavior. Although there is a lack of scientific knowledge on this subject, neutering is often conducted to get a more trainable and less aggressive dog. The aim of this study is to examine castration from an ethological perspective and to reveal possible behavioural changes after castration related to breed. An online study was conducted consisting of two different questionnaires about the dog’s personality. A total of 242 dog owners participated in the study concerning specific behaviour problems (n = 130 intact and 112 neutered males). Another 211 owners of males (n = 115 intact, 96 neutered) completed the questionnaire about personality traits (dog sociability, trainability, emotional stability and extraversion) based on Turcsán et al. 2011. Four breed categories were formed following Parker et al. (2017): shepherds, retrievers, terriers, and hunting Dogs. Our study reveals that intact males are bolder than neutered males. Intact males have lower aggression scores than neutered ones. Castrated males show significantly more panic behavior (multinomial logistic Regression, p = 0.04). Intact shepherds are bolder than neutered ones (Mann-Whiney-U-Test: p = 0.03) and intact terriers are bolder than neutered terriers (Mann-Whitney-U = Test: p = 0.04). Intact terriers scored lower for aggression than neutered ones. With these results and the data of previous studies, we question castration to modify behavior, as a measure for reproductive control and as a preventive measure against diseases.
对狗进行阉割通常是作为预防疾病和不良行为的措施。虽然出于医疗原因,雌性狗最好被阉割,但雄性狗的主人希望它们的行为有所改善。虽然在这个问题上缺乏科学知识,但绝育通常是为了得到一只更容易训练、更少攻击性的狗。本研究的目的是从动物行为学的角度来研究阉割,并揭示阉割后与繁殖有关的可能的行为变化。一项在线研究由两份不同的关于狗狗性格的问卷组成。共有242名狗主人参与了有关特定行为问题的研究(n = 130名未受影响的公狗和112名阉割的公狗)。另外211名雄性狗主人(n = 115名完整狗主人,96名绝育狗主人)完成了基于Turcsán等人2011年的人格特征问卷(狗的社交性、可训练性、情绪稳定性和外向性)。在Parker等人(2017)之后,形成了四个品种类别:牧羊犬,寻回犬,梗犬和猎犬。我们的研究表明,完整的雄性比绝育的雄性更勇敢。完整的雄性比阉割的雄性攻击性得分低。阉割的男性表现出更多的恐慌行为(多项logistic回归,p = 0.04)。完整的牧羊犬比绝育的牧羊犬胆子更大(Mann-Whitney-U = Test: p = 0.03),完整的梗比绝育梗胆子更大(Mann-Whitney-U = Test: p = 0.04)。完好无损的梗犬攻击性得分低于绝育梗犬。根据这些结果和以前的研究数据,我们质疑阉割是否可以改变行为,作为一种生殖控制和预防疾病的措施。
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引用次数: 0
Investigation of In-Vivo Effect of St. John’s Wort (Hypericum Perforatum) In Lung Cancer 贯叶连翘(St. John’s Wort,贯叶连翘)对肺癌体内作用的研究
IF 2.2 Q1 VETERINARY SCIENCES Pub Date : 2021-01-01 DOI: 10.13188/2325-4645.1000056
EO Serinan, OE Gökbayrak
Due to limitations in the treatment of lung cancer, finding natural compounds from plants can provide an alternative treatment for lung cancer. St. John’s Wort (SJW) has anti-proliferative and pro-apoptotic properties that can be used in lung cancer treatment. The aim of this study is to explore antitumor effect of SJW in lung cancer in vivo animal model. 35 animals; 7 animals in each group were randomized as control, Doxorubicin, SJW early treatment, SJW treatment, and doxorubicin+SJW groups. After 7 days sacrification was performed. Tumor diameter did not show statistically significant change but in all four-group compared with control group; tumor tissue showed prominent necrosis and apoptosis. No histologic changes observed in other tissues. Biochemistry did not show organ insufficiency. SJW is shown to have antitumoral effect in subcutaneous xenograft lung cancer in vivo model in nude mice. Dose was obtained comparing with DOX. In combination with DOX, there were no synergistic increase in anti-tumo effect. SJW might be a candidate antineoplastic supplementation in lung cancer.
由于肺癌治疗的局限性,从植物中寻找天然化合物可以为肺癌提供另一种治疗方法。圣约翰草(SJW)具有抗增殖和促细胞凋亡的特性,可用于肺癌治疗。本研究旨在探讨SJW对肺癌体内动物模型的抗肿瘤作用。35动物;每组7只动物随机分为对照组、阿霉素、SJW早期治疗组、SJW治疗组和阿霉素+SJW组。7天后进行祭祀。与对照组比较,四组患者肿瘤直径变化无统计学意义;肿瘤组织坏死、凋亡明显。其他组织未见组织学改变。生化未见器官功能不全。结果表明,SJW对裸鼠皮下移植肺癌有一定的抗肿瘤作用。与DOX比较得到剂量。与DOX联合使用时,抗肿瘤作用无增效性增加。SJW可能是肺癌的一种候选抗肿瘤补充剂。
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引用次数: 0
Synchronous Diaphragmatic Flutter in a Lactating Mare 哺乳期母马的同步膈肌颤振
IF 2.2 Q1 VETERINARY SCIENCES Pub Date : 2020-12-30 DOI: 10.13188/2325-4645.1000050
J. Maxwell
The mare presented subdued, lethargic and with a lowered head. There was little clinical evidence of sweating and the mare was assessed as not being markedly dehydrated. The mare walked into the hospital and was steady on its feet, but exhibited breathing difficulties. On physical examination, the mare was observed to have very rapid abdominal respiration and on auscultation of the chest, was found to have synchronous heart and respiratory rates of nearly 60 per minute, causing the mare considerable respiratory distress and an exaggerated respiratory sound.
那匹母马显得无精打采,低着头。几乎没有临床证据表明出汗,母马被评估为没有明显脱水。这匹母马走进了医院,它的脚很稳定,但表现出呼吸困难。在体格检查中,观察到母马有非常快速的腹部呼吸,在胸部听诊中,发现心脏和呼吸频率同步接近每分钟60次,导致母马严重的呼吸窘迫和夸张的呼吸声。
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引用次数: 0
Review on Triclabendazole Resistance in Fasciola 片形吸虫对三氯苯达唑的耐药性研究进展
IF 2.2 Q1 VETERINARY SCIENCES Pub Date : 2020-12-30 DOI: 10.13188/2325-4645.1000051
Warkaw Merachew, T. Alemneh
The control of Fasciolosis can be achieved by application of anthelmintic drugs, elimination of the number of intermediate hosts and reduction of exposure to infection. Triclabendazole, which is a member of Benzimidazole, is most recommended and effective way of controlling fasciolosis in animals and humans that can kill both mature (adult) and immature liver flukes. This drug have able to penetrate the tegument of Fasciola (F) hepatica by diffusion, and the fluke is able to sulfoxidate the drug to the active sulfoxide metabolite which binds to β-tubulin and thus inhibit the formation of microtubules that are components of cytoskeleton of the parasite. However, in recent year, resistance of Triclabendazole is reported in animals and humans in different regions of the world. Resistance has likely appeared due to a generally poor understanding of liver fluke biology by farmers and con-founding factors, such as incorrect dosing, inappropriate product choice, and lack of testing for efficacy. These conditions may lead to reduced diffusion and metabolism of the drug, change efflux pump activity and changes in the target molecule that can reduce the effectiveness of Triclabendazole. Both in-vivo and in-vitro methods, like Faecal Egg Count Reduction Test (FECRT) and the Egg Hatch Assay (EHA), respectively, can help to investigate the resistance of Triclabendazole. Administration of dual active flukicide drugs, development of vaccines, implementation of Fasciola control methods other than Triclabendazole, and use of accurate dosage at appropriate time can help to reduce the incidence of Triclabendazole resistance.
片形虫病的控制可以通过应用驱虫药、消除中间宿主数量和减少感染暴露来实现。三氯咪唑是苯并咪唑的一种成员,是控制动物和人类片形吸虫病的最推荐和有效的方法,它可以杀死成熟(成人)和未成熟的肝吸虫。这种药物能够通过扩散穿透肝片形吸虫的被膜,并且吸虫能够将药物的亚砜分解为与β-微管蛋白结合的活性亚砜代谢物,从而抑制微管的形成,而微管是寄生虫细胞骨架的组成部分。然而,近年来,在世界不同地区的动物和人类中报道了三氯咪唑的耐药性。耐药性的出现可能是由于农民对肝吸虫生物学的普遍了解不足,以及一些混杂因素,如不正确的剂量、不适当的产品选择和缺乏疗效测试。这些情况可能导致药物的扩散和代谢减少,改变外排泵活性和靶分子的变化,从而降低三氯咪唑的有效性。体内和体外方法,如粪卵计数减少试验(FECRT)和卵孵化试验(EHA),都可以帮助研究三氯咪唑的耐药性。使用双活性杀氟剂药物、研制疫苗、实施除三氯苯达唑以外的片形虫控制方法、适时使用准确剂量,有助于降低三氯苯达唑耐药发生率。
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引用次数: 8
期刊
International Journal of Veterinary Science and Medicine
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