Nanotechnology is a fast-growing technology that plays an important great impact on various fields of therapeutic applications. It is capable for solving several problems related to animal health and production. There are different nano-systems such as liposomes, metallic nanoparticles, polymeric micelles, polymeric nanospheres, functionalized fullerenes, carbon nanotubes, dendrimers, polymer-coated nanocrystals and nanoshells. In this review, we mentioned different methods for the preparation and characterization of nanoparticles. This review is concerned mainly on nanoparticle systems for antibiotic delivery which suffer from poor bioavailability and many side effects. Nanoparticles are characterized by many features include their minimal size, colossal surface zone to mass extent. The development of antimicrobials in nanoparticle systems is considered an excellent alternative delivery system for antimicrobials for the treatment of microbial diseases by increasing therapeutic effect and overcoming the side effects. In this paper, we reviewed some antimicrobial nanoparticle preparations and we focused on florfenicol and neomycin nanoparticle preparations as well as chitosan and silver nanoparticles preparations to prepare, characterize and compare their different pharmacological effects.
{"title":"Application of some nanoparticles in the field of veterinary medicine.","authors":"Fady Sayed Youssef, Hossny Awad El-Banna, Hesham Youssef Elzorba, Ahmed Mohamed Galal","doi":"10.1080/23144599.2019.1691379","DOIUrl":"10.1080/23144599.2019.1691379","url":null,"abstract":"<p><p>Nanotechnology is a fast-growing technology that plays an important great impact on various fields of therapeutic applications. It is capable for solving several problems related to animal health and production. There are different nano-systems such as liposomes, metallic nanoparticles, polymeric micelles, polymeric nanospheres, functionalized fullerenes, carbon nanotubes, dendrimers, polymer-coated nanocrystals and nanoshells. In this review, we mentioned different methods for the preparation and characterization of nanoparticles. This review is concerned mainly on nanoparticle systems for antibiotic delivery which suffer from poor bioavailability and many side effects. Nanoparticles are characterized by many features include their minimal size, colossal surface zone to mass extent. The development of antimicrobials in nanoparticle systems is considered an excellent alternative delivery system for antimicrobials for the treatment of microbial diseases by increasing therapeutic effect and overcoming the side effects. In this paper, we reviewed some antimicrobial nanoparticle preparations and we focused on florfenicol and neomycin nanoparticle preparations as well as chitosan and silver nanoparticles preparations to prepare, characterize and compare their different pharmacological effects.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"7 1","pages":"78-93"},"PeriodicalIF":2.2,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2019.1691379","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37603345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-17eCollection Date: 2019-01-01DOI: 10.1080/23144599.2019.1694307
Na'imatu A Sani, Mohammed U Kawu, Ibrahim G Bako
Inadequacy of milk supply to meet the increasing human population coupled with a decreasing livestock population has necessitated the need for a potent galactogoue. The aim of this study was to compare the lactogenic effects of Launaea taraxacifolia (PLT) and resveratrol in Wistar rats. After parturition, 25 primiparous female Wistar rats were randomly allocated into five groups of 5 dams each. Dams in groups I, II, III, IV and V were administered distilled water (DW: 2 ml/kg), metochlopromide (MET: 15 mg/kg), resveratrol (RES: 5 mg/kg), n-hexane leaf fraction of L. taraxacifolia (PLT: 333 mg/kg) and the combination of RES + PLT (CO: 5 mg + 333 mg/kg); respectively, for 12 days. Pup weight gain was used to quantify milk yield. Serum was harvested from the dams and assayed for prolactin and oxytocin. The PLT and CO groups had significantly higher (p < 0.05) milk yield than DW group. Serum concentration of prolactin was significantly higher (p < 0.05) in the PLT group, while the combination group had the highest (p < 0.05) concentration of oxytocin compared to DW group. In conclusion, L. taraxacifolia and resveratrol exhibited galactopoietic potentials individually by stimulating hyperprolactinaemia, while their combination increased milk production by increasing serum oxytocin activity.
{"title":"Effects of <i>Launaea taraxacifolia</i> and resveratrol on milk yield and serum prolactin and oxytocin levels: a lactogenic study.","authors":"Na'imatu A Sani, Mohammed U Kawu, Ibrahim G Bako","doi":"10.1080/23144599.2019.1694307","DOIUrl":"https://doi.org/10.1080/23144599.2019.1694307","url":null,"abstract":"<p><p>Inadequacy of milk supply to meet the increasing human population coupled with a decreasing livestock population has necessitated the need for a potent galactogoue. The aim of this study was to compare the lactogenic effects of <i>Launaea taraxacifolia</i> (PLT) and resveratrol in Wistar rats. After parturition, 25 primiparous female Wistar rats were randomly allocated into five groups of 5 dams each. Dams in groups I, II, III, IV and V were administered distilled water (DW: 2 ml/kg), metochlopromide (MET: 15 mg/kg), resveratrol (RES: 5 mg/kg), <i>n</i>-hexane leaf fraction of <i>L. taraxacifolia</i> (PLT: 333 mg/kg) and the combination of RES + PLT (CO: 5 mg + 333 mg/kg); respectively, for 12 days. Pup weight gain was used to quantify milk yield. Serum was harvested from the dams and assayed for prolactin and oxytocin. The PLT and CO groups had significantly higher (<i>p</i> < 0.05) milk yield than DW group. Serum concentration of prolactin was significantly higher (<i>p</i> < 0.05) in the PLT group, while the combination group had the highest (<i>p</i> < 0.05) concentration of oxytocin compared to DW group. In conclusion, <i>L. taraxacifolia</i> and resveratrol exhibited galactopoietic potentials individually by stimulating hyperprolactinaemia, while their combination increased milk production by increasing serum oxytocin activity.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"7 1","pages":"71-77"},"PeriodicalIF":2.2,"publicationDate":"2019-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2019.1694307","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37603402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-03eCollection Date: 2019-01-01DOI: 10.1080/23144599.2019.1691378
Karima M Fahim, Elshaimaa Ismael, Hanan S Khalefa, Heba S Farag, Dalia A Hamza
The study was conducted to estimate the prevalence of Escherichia coli (E. coli) in sub-clinically mastitic (SCM) animals, and in wild and migratory birds which may act as reservoir disseminating such pathogen. Farm hygiene, management and milking procedures were listed through a questionnaire. Thirty lactating cows and 15 lactating buffaloes from five small-scale dairy farms were randomly selected and screened for subclinical mastitis (SCM) using California Mastitis Test (CMT) and somatic cell count (SCC). In addition, 80 teat skin swabs, 5 drinking water samples and 38 wild and migratory bird faecal matter were also collected. All samples were processed for E. coli isolation by culturing on Levine's Eosin Methylene Blue (L-EMB) agar, followed by purification and biochemical identification. Positive samples were subjected to molecular identification and serotyping. In addition, the presence of extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing E. coli have been reported by antimicrobial sensitivity testing. Escherichia coli were isolated from 7.7%, 50% and 50% of the positive CMT cows' quarters, cows' composite and buffaloes' composite milk samples, respectively. In addition, 14% of cows' teats, 20% of water samples, 70% of faecal matter from wild bird, and 33.3% of faecal matter from migratory waterfowls were carrying E. coli. Serotyping, antibiotic-resistant pattern and phylogenetic analysis have pointed the bearable implication of milking hygiene and wild birds in disseminating E. coli strains causing intramammary infections.
{"title":"Isolation and characterization of <i>E. coli</i> strains causing intramammary infections from dairy animals and wild birds.","authors":"Karima M Fahim, Elshaimaa Ismael, Hanan S Khalefa, Heba S Farag, Dalia A Hamza","doi":"10.1080/23144599.2019.1691378","DOIUrl":"https://doi.org/10.1080/23144599.2019.1691378","url":null,"abstract":"<p><p>The study was conducted to estimate the prevalence of <i>Escherichia coli</i> (<i>E. coli</i>) in sub-clinically mastitic (SCM) animals, and in wild and migratory birds which may act as reservoir disseminating such pathogen. Farm hygiene, management and milking procedures were listed through a questionnaire. Thirty lactating cows and 15 lactating buffaloes from five small-scale dairy farms were randomly selected and screened for subclinical mastitis (SCM) using California Mastitis Test (CMT) and somatic cell count (SCC). In addition, 80 teat skin swabs, 5 drinking water samples and 38 wild and migratory bird faecal matter were also collected. All samples were processed for <i>E. coli</i> isolation by culturing on Levine's Eosin Methylene Blue (L-EMB) agar, followed by purification and biochemical identification. Positive samples were subjected to molecular identification and serotyping. In addition, the presence of extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing <i>E. coli</i> have been reported by antimicrobial sensitivity testing. <i>Escherichia coli</i> were isolated from 7.7%, 50% and 50% of the positive CMT cows' quarters, cows' composite and buffaloes' composite milk samples, respectively. In addition, 14% of cows' teats, 20% of water samples, 70% of faecal matter from wild bird, and 33.3% of faecal matter from migratory waterfowls were carrying <i>E. coli</i>. Serotyping, antibiotic-resistant pattern and phylogenetic analysis have pointed the bearable implication of milking hygiene and wild birds in disseminating <i>E. coli</i> strains causing intramammary infections.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"7 1","pages":"61-70"},"PeriodicalIF":2.2,"publicationDate":"2019-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2019.1691378","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37459742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-21eCollection Date: 2019-01-01DOI: 10.1080/23144599.2019.1641899
Karina Lezama-García, Chiara Mariti, Daniel Mota-Rojas, Julio Martínez-Burnes, Hugo Barrios-García, Angelo Gazzano
Mammalian parental care, in most of the cases, is given by the female, who provides food, warmth, and protection. In domestic dogs, maternal behaviour shown by the dam mainly consists of contact, nursing, grooming/licking, play, punishment, thermoregulation, and motion. Peer-reviewed literature published between 1952 and 2018 was retrieved from CAB Abstracts, PubMed, ISI Web of Knowledge, Scopus and book chapters. Keywords for this search included the following terms: behaviour, bonding, altricial, precocial, offspring, maternal, whelping, nursing, domestic dogs, female dog, aggression, puppies, anogenital licking. In this review, we reported and discussed scientific information about maternal behaviour in domestic bitches, comparing altricial vs precocial species; the importance of the bonding, grooming/licking and nursing, and their impacts on puppies' behaviour; altered maternal behaviours such as aggression, cannibalism, rejection, and also the relation between hormones and maternal care behaviours. We concluded that the level of interactions between the dam and the puppies influences the physiological, cognitive and behavioural development of the litter, and the main hormones in the bitch for inducing maternal care behaviours are estradiol, oxytocin, prolactin and progesterone.
哺乳动物的父母照顾大多由雌性提供,雌性提供食物、温暖和保护。在家犬中,母犬的母性行为主要包括接触、哺乳、梳理/舔舐、玩耍、惩罚、体温调节和运动。从 CAB Abstracts、PubMed、ISI Web of Knowledge、Scopus 和书籍章节中检索了 1952 年至 2018 年间发表的同行评审文献。此次检索的关键词包括以下术语:行为、结合、初生、前社群、后代、母性、产仔、哺乳、家犬、母犬、攻击性、幼犬、舔舐肛门。在这篇综述中,我们报告并讨论了有关家犬母狗母性行为的科学信息,比较了早产与早产物种;亲子关系、梳理/舔舐和哺乳的重要性及其对幼犬行为的影响;改变的母性行为,如攻击、食人、拒绝,以及荷尔蒙与母性护理行为之间的关系。我们得出的结论是,母狗与幼犬之间的互动程度会影响幼犬的生理、认知和行为发展,而母狗体内诱发母性护理行为的主要激素是雌二醇、催产素、催乳素和孕酮。
{"title":"Maternal behaviour in domestic dogs.","authors":"Karina Lezama-García, Chiara Mariti, Daniel Mota-Rojas, Julio Martínez-Burnes, Hugo Barrios-García, Angelo Gazzano","doi":"10.1080/23144599.2019.1641899","DOIUrl":"https://doi.org/10.1080/23144599.2019.1641899","url":null,"abstract":"<p><p>Mammalian parental care, in most of the cases, is given by the female, who provides food, warmth, and protection. In domestic dogs, maternal behaviour shown by the dam mainly consists of contact, nursing, grooming/licking, play, punishment, thermoregulation, and motion. Peer-reviewed literature published between 1952 and 2018 was retrieved from CAB Abstracts, PubMed, ISI Web of Knowledge, Scopus and book chapters. Keywords for this search included the following terms: behaviour, bonding, altricial, precocial, offspring, maternal, whelping, nursing, domestic dogs, female dog, aggression, puppies, anogenital licking. In this review, we reported and discussed scientific information about maternal behaviour in domestic bitches, comparing altricial vs precocial species; the importance of the bonding, grooming/licking and nursing, and their impacts on puppies' behaviour; altered maternal behaviours such as aggression, cannibalism, rejection, and also the relation between hormones and maternal care behaviours. We concluded that the level of interactions between the dam and the puppies influences the physiological, cognitive and behavioural development of the litter, and the main hormones in the bitch for inducing maternal care behaviours are estradiol, oxytocin, prolactin and progesterone.</p>","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"7 1","pages":"20-30"},"PeriodicalIF":2.8,"publicationDate":"2019-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6776987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141727944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/23144599.2019.1678315
E. Marijani, H. Charo-Karisa, G. J. B. Gnonlonfin, E. Kigadye, S. Okoth
ABSTRACT This study evaluated the effect of dietary aflatoxin B1 (AFB1) on growth, milt and egg quality in matured Nile tilapia (Oreochromis niloticus). Triplicate groups of Nile tilapia (initial body weight 24.1 ± 2.6 g) were fed with either of four diets (Diets 1 to 4) designed to contain 0, 20, 200 and 2000 μg AFB1 kg−1 diets for 24 weeks. After 24 weeks of AFB1 exposure, growth was significantly (P <0.05) different between the control and the AFB1 exposed treatments in both sexes. No significant differences were observed in 17β-oestradiol, absolute fecundity, oocytes volume and diameters between AFB1 exposure groups and the control group. However, we observed a significant reduction in relative fecundity and gonad somatic index (GSI) in females fed 2000 μg AFB1 kg−1 diet. On the other hand, we observed significant differences (P <0.05) in gonadosomatic index (GSI), testosterone, milt count and motility between males in the control group and AFB1 treatments. We conclude that rearing Nile tilapia with aflatoxin-contaminated diets for a prolonged period affects milt quality, fecundity (at higher doses) and growth performance. This implies that for optimal seed production, provision of aflatoxin free diets should be part of the management practices in Nile tilapia hatcheries.
摘要本试验旨在研究饲料中添加黄曲霉毒素B1 (AFB1)对尼罗罗非鱼(Oreochromis niloticus)生长、乳质和蛋品质的影响。选取初始体重为24.1±2.6 g的尼罗罗非鱼,分别饲喂AFB1 kg - 1含量为0、20、200和2000 μg的饲料(饲料1 ~饲料4),饲喂24周。AFB1暴露24周后,两性AFB1暴露处理与对照的生长差异显著(P <0.05)。AFB1暴露组与对照组在17β-雌二醇、绝对繁殖力、卵母细胞体积和直径方面均无显著差异。然而,我们观察到,饲喂2000 μg AFB1 kg - 1日粮的雌性相对繁殖力和性腺体细胞指数(GSI)显著降低。另一方面,我们观察到对照组和AFB1治疗组男性在性腺指数(GSI)、睾酮、milt计数和运动性方面存在显著差异(P <0.05)。我们得出的结论是,长期用黄曲霉毒素污染的饲料饲养尼罗罗非鱼会影响鱼的品质、繁殖力(在较高剂量下)和生长性能。这意味着,为了获得最佳的种子产量,提供不含黄曲霉毒素的饲料应成为尼罗罗非鱼孵化场管理实践的一部分。
{"title":"Effects of aflatoxin B1 on reproductive performance of farmed Nile tilapia","authors":"E. Marijani, H. Charo-Karisa, G. J. B. Gnonlonfin, E. Kigadye, S. Okoth","doi":"10.1080/23144599.2019.1678315","DOIUrl":"https://doi.org/10.1080/23144599.2019.1678315","url":null,"abstract":"ABSTRACT This study evaluated the effect of dietary aflatoxin B1 (AFB1) on growth, milt and egg quality in matured Nile tilapia (Oreochromis niloticus). Triplicate groups of Nile tilapia (initial body weight 24.1 ± 2.6 g) were fed with either of four diets (Diets 1 to 4) designed to contain 0, 20, 200 and 2000 μg AFB1 kg−1 diets for 24 weeks. After 24 weeks of AFB1 exposure, growth was significantly (P <0.05) different between the control and the AFB1 exposed treatments in both sexes. No significant differences were observed in 17β-oestradiol, absolute fecundity, oocytes volume and diameters between AFB1 exposure groups and the control group. However, we observed a significant reduction in relative fecundity and gonad somatic index (GSI) in females fed 2000 μg AFB1 kg−1 diet. On the other hand, we observed significant differences (P <0.05) in gonadosomatic index (GSI), testosterone, milt count and motility between males in the control group and AFB1 treatments. We conclude that rearing Nile tilapia with aflatoxin-contaminated diets for a prolonged period affects milt quality, fecundity (at higher doses) and growth performance. This implies that for optimal seed production, provision of aflatoxin free diets should be part of the management practices in Nile tilapia hatcheries.","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"15 1","pages":"35 - 42"},"PeriodicalIF":2.2,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/23144599.2019.1678315","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72537372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/23144599.2019.1624299
M. Opiyo, J. Jumbe, C. Ngugi, H. Charo-Karisa
ABSTRACT Poor culture conditions in low input ponds make fish highly susceptible to infectious pathogens which lead to diseases and mortalities yet the effects of probiotics on immunity, gut microbiota and microbiological quality of fish in low input ponds are unknown. Nile tilapia, Oreochromis niloticus fingerlings (40 g) were randomly stocked at 50 fish m−3 in 1.25 m3 cages in low input ponds. The fish were fed on diets supplemented with either Saccharomyces cerevisiae (1 × 1010 CFU g−1) or Bacillus subtilis (1 × 109 CFU g−1) at six levels: Diet 0 (No probiotic); S. cerevisiae at 2 g kg−1 (Diet 1); 4 g kg−1 (Diet 2) and 6 g kg−1 (Diet 3) and B. subtilis at 5 g kg−1 (Diet 4); 10 g kg−1 (Diet 5) and 15 g kg−1 (Diet 6) for 180 days. Results indicate that hemato-immunological parameters (hemoglobin (Hb), red blood cells (RBC), white blood cells (WBC) serum protein, albumin, globulin and lysozyme activity) were significantly higher in fish fed on probiotic treated diets compared to the control (P < 0.05). On the contrary, fish fed on Diet 6 presented significantly similar Hb and globulin values compared to the control (P > 0.05). Additionally, fish fed on probiotic treated diets retained the probiotics in their guts and lower microbial load was realized in their muscle (P < 0.05). In conclusion, B. subtilis and S. cerevisiae supplementation in diets of Nile tilapia reared in low input ponds improves immunity, manipulates gut microbiota and enhances fish flesh quality.
低投入池塘养殖条件差,使鱼类极易感染传染性病原体,导致疾病和死亡,而益生菌对低投入池塘鱼类免疫力、肠道菌群和微生物质量的影响尚不清楚。尼罗罗非鱼(Oreochromis niloticus)鱼苗(40 g)随机放养在1.25 m3网箱中,放养量为50鱼m - 3。在饲料中添加6个水平的酿酒酵母(1 × 1010 CFU g−1)或枯草芽孢杆菌(1 × 109 CFU g−1):饲料0(不添加益生菌);酿酒酵母2 g kg−1(饲料1);4 g kg−1(饲料2)和6 g kg−1(饲料3),枯草芽孢杆菌为5 g kg−1(饲料4);10 g kg−1(饲粮5)和15 g kg−1(饲粮6),连续180 d。结果表明,饲料中添加益生菌的鱼的血液免疫指标(血红蛋白(Hb)、红细胞(RBC)、白细胞(WBC)、血清蛋白、白蛋白、球蛋白和溶菌酶活性)均显著高于对照组(P < 0.05)。相反,饲粮6的血红蛋白和球蛋白值与对照组显著相似(P > 0.05)。此外,饲喂益生菌饲料的鱼在肠道中保留了益生菌,在肌肉中实现了较低的微生物负荷(P < 0.05)。综上所述,在低投入池塘养殖的尼罗罗非鱼饲料中添加枯草芽孢杆菌和酿酒酵母可提高其免疫力,控制肠道菌群,改善鱼肉品质。
{"title":"Dietary administration of probiotics modulates non-specific immunity and gut microbiota of Nile tilapia (Oreochromis niloticus) cultured in low input ponds","authors":"M. Opiyo, J. Jumbe, C. Ngugi, H. Charo-Karisa","doi":"10.1080/23144599.2019.1624299","DOIUrl":"https://doi.org/10.1080/23144599.2019.1624299","url":null,"abstract":"ABSTRACT Poor culture conditions in low input ponds make fish highly susceptible to infectious pathogens which lead to diseases and mortalities yet the effects of probiotics on immunity, gut microbiota and microbiological quality of fish in low input ponds are unknown. Nile tilapia, Oreochromis niloticus fingerlings (40 g) were randomly stocked at 50 fish m−3 in 1.25 m3 cages in low input ponds. The fish were fed on diets supplemented with either Saccharomyces cerevisiae (1 × 1010 CFU g−1) or Bacillus subtilis (1 × 109 CFU g−1) at six levels: Diet 0 (No probiotic); S. cerevisiae at 2 g kg−1 (Diet 1); 4 g kg−1 (Diet 2) and 6 g kg−1 (Diet 3) and B. subtilis at 5 g kg−1 (Diet 4); 10 g kg−1 (Diet 5) and 15 g kg−1 (Diet 6) for 180 days. Results indicate that hemato-immunological parameters (hemoglobin (Hb), red blood cells (RBC), white blood cells (WBC) serum protein, albumin, globulin and lysozyme activity) were significantly higher in fish fed on probiotic treated diets compared to the control (P < 0.05). On the contrary, fish fed on Diet 6 presented significantly similar Hb and globulin values compared to the control (P > 0.05). Additionally, fish fed on probiotic treated diets retained the probiotics in their guts and lower microbial load was realized in their muscle (P < 0.05). In conclusion, B. subtilis and S. cerevisiae supplementation in diets of Nile tilapia reared in low input ponds improves immunity, manipulates gut microbiota and enhances fish flesh quality.","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"21 1","pages":"1 - 9"},"PeriodicalIF":2.2,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83073464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/23144599.2019.1689630
K. Abdel-moein, Hala M. Zaher
ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging pathogen causing serious public health threats. This study was conducted to investigate the occurrence of multidrug-resistant MRSA among apparently healthy farm animals to shed the light on the potential role of these animals as a reservoir for such pathogen. For this purpose, 195 nasal swabs from apparently healthy farm animals (52 sheep, 51 goats, 47 cattle and 45 buffalo) were screened for multidrug-resistant MRSA. MRSA was isolated using a selective chromogenic medium and identified by colonial characters, Gram’s stain films, conventional biochemical tests, coagulase test, resistance to cefoxitin and amplification of nuc and mecA genes. The antimicrobial susceptibility testing profile was performed by disk diffusion method to identify multidrug-resistant MRSA. Of 195 samples, 7 yielded MRSA with an overall prevalence 3.6%, whereas the prevalence rates were 3.8%, 3.9%, 4.3% and 2.2% for sheep, goats, cattle and buffalo, respectively. All MRSA isolates were multidrug-resistant strains. The phylogenetic analysis of 2 mecA gene sequences from the obtained isolates revealed that both sequences were clustered in the same clade with those derived from human clinical cases from different countries to highlight the public health burden of such strains. The distribution of multidrug-resistant MRSA among all examined farm animal species being apparently healthy points out that farm animals could represent a potential reservoir for multidrug-resistant MRSA with public health implications.
{"title":"Occurrence of multidrug-resistant methicillin-resistant Staphylococcus aureus among healthy farm animals: a public health concern","authors":"K. Abdel-moein, Hala M. Zaher","doi":"10.1080/23144599.2019.1689630","DOIUrl":"https://doi.org/10.1080/23144599.2019.1689630","url":null,"abstract":"ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging pathogen causing serious public health threats. This study was conducted to investigate the occurrence of multidrug-resistant MRSA among apparently healthy farm animals to shed the light on the potential role of these animals as a reservoir for such pathogen. For this purpose, 195 nasal swabs from apparently healthy farm animals (52 sheep, 51 goats, 47 cattle and 45 buffalo) were screened for multidrug-resistant MRSA. MRSA was isolated using a selective chromogenic medium and identified by colonial characters, Gram’s stain films, conventional biochemical tests, coagulase test, resistance to cefoxitin and amplification of nuc and mecA genes. The antimicrobial susceptibility testing profile was performed by disk diffusion method to identify multidrug-resistant MRSA. Of 195 samples, 7 yielded MRSA with an overall prevalence 3.6%, whereas the prevalence rates were 3.8%, 3.9%, 4.3% and 2.2% for sheep, goats, cattle and buffalo, respectively. All MRSA isolates were multidrug-resistant strains. The phylogenetic analysis of 2 mecA gene sequences from the obtained isolates revealed that both sequences were clustered in the same clade with those derived from human clinical cases from different countries to highlight the public health burden of such strains. The distribution of multidrug-resistant MRSA among all examined farm animal species being apparently healthy points out that farm animals could represent a potential reservoir for multidrug-resistant MRSA with public health implications.","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"18 1","pages":"55 - 60"},"PeriodicalIF":2.2,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89181850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/23144599.2019.1680044
I. Hernández-Ávalos, D. Mota-Rojas, P. Mora-Medina, J. Martínez-Burnes, Alejandro Casas Alvarado, Antonio Verduzco-Mendoza, K. Lezama-García, A. Olmos-Hernández
ABSTRACT In light of the need to perform surgical techniques and the importance of animal welfare because of acute pain, the objectives of the veterinary anaesthetists are to manage muscle relaxation and adequate analgesia in order to conserve a balance in the autonomic nervous system, enhance the action of the parasympathetic system in the face of the emerging action of the sympathetic portion provoked by the surgeon, and maintain a balance among them. The aim of the present review is to describe different evaluation criteria for acute pain using unidimensional and multidimensional scales, correlating these findings to parasympathetic tone activity (PTA) and bispectral index (BIS) assessment, to conduct an objective evaluation of pain that patients (dog or cat) perceives, in order to administrate an adequate analgesic treatment in each case. In conclusion, this integral, objective evaluation will allow veterinarians – especially anaesthesiologists – to improve the management of pain in the patients.
{"title":"Review of different methods used for clinical recognition and assessment of pain in dogs and cats","authors":"I. Hernández-Ávalos, D. Mota-Rojas, P. Mora-Medina, J. Martínez-Burnes, Alejandro Casas Alvarado, Antonio Verduzco-Mendoza, K. Lezama-García, A. Olmos-Hernández","doi":"10.1080/23144599.2019.1680044","DOIUrl":"https://doi.org/10.1080/23144599.2019.1680044","url":null,"abstract":"ABSTRACT In light of the need to perform surgical techniques and the importance of animal welfare because of acute pain, the objectives of the veterinary anaesthetists are to manage muscle relaxation and adequate analgesia in order to conserve a balance in the autonomic nervous system, enhance the action of the parasympathetic system in the face of the emerging action of the sympathetic portion provoked by the surgeon, and maintain a balance among them. The aim of the present review is to describe different evaluation criteria for acute pain using unidimensional and multidimensional scales, correlating these findings to parasympathetic tone activity (PTA) and bispectral index (BIS) assessment, to conduct an objective evaluation of pain that patients (dog or cat) perceives, in order to administrate an adequate analgesic treatment in each case. In conclusion, this integral, objective evaluation will allow veterinarians – especially anaesthesiologists – to improve the management of pain in the patients.","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"70 1","pages":"43 - 54"},"PeriodicalIF":2.2,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77398539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/23144599.2019.1637046
A. Samir, Amany Adel, A. Arafa, H. Sultan, H. A. Hussein Ahmed
ABSTRACT Since the introduction of H9N2 low pathogenic avian influenza virus in Egypt, it became an endemic disease causing considerable economic losses in different poultry sectors especially in the presence of other secondary bacterial and viral infections. The H9N2 viruses in Egypt are in continuous evolution that needs deep analysis for their evolution pattern based on the genetic constitutions of the pathogenic determinant genes (HA, PB2, PB1, PA, and NS). In this work, samples were collected from the backyard chickens from 3 Egyptian governorates. Five selected viruses were sequenced and analyzed for the hemagglutinin gene which showed genetic relatedness to the Asian G1 lineage group B, similar to the circulating H9N2 viruses in Egypt since 2013. The sequence for PB2, PB1, PA, HA and NS genes of the selected five viruses indicate a natural re-assortment event with recent Eurasian subtypes and similar to Egyptian H9N2 virus isolated from pigeon in Egypt during 2014. The Egyptian viruses of our study possess amino acids signatures including S42, V127, L550, L672 and V504 in the internal genes NS1, PA, and PB2, of respectively of an impact on virus transmission and replication. This work indicates that the H9N2 is in continuous evolution with alarming to the reassortment occurrence.
{"title":"Molecular pathogenic and host range determinants of reassortant Egyptian low pathogenic avian influenza H9N2 viruses from backyard chicken","authors":"A. Samir, Amany Adel, A. Arafa, H. Sultan, H. A. Hussein Ahmed","doi":"10.1080/23144599.2019.1637046","DOIUrl":"https://doi.org/10.1080/23144599.2019.1637046","url":null,"abstract":"ABSTRACT Since the introduction of H9N2 low pathogenic avian influenza virus in Egypt, it became an endemic disease causing considerable economic losses in different poultry sectors especially in the presence of other secondary bacterial and viral infections. The H9N2 viruses in Egypt are in continuous evolution that needs deep analysis for their evolution pattern based on the genetic constitutions of the pathogenic determinant genes (HA, PB2, PB1, PA, and NS). In this work, samples were collected from the backyard chickens from 3 Egyptian governorates. Five selected viruses were sequenced and analyzed for the hemagglutinin gene which showed genetic relatedness to the Asian G1 lineage group B, similar to the circulating H9N2 viruses in Egypt since 2013. The sequence for PB2, PB1, PA, HA and NS genes of the selected five viruses indicate a natural re-assortment event with recent Eurasian subtypes and similar to Egyptian H9N2 virus isolated from pigeon in Egypt during 2014. The Egyptian viruses of our study possess amino acids signatures including S42, V127, L550, L672 and V504 in the internal genes NS1, PA, and PB2, of respectively of an impact on virus transmission and replication. This work indicates that the H9N2 is in continuous evolution with alarming to the reassortment occurrence.","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"1 1","pages":"10 - 19"},"PeriodicalIF":2.2,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80351166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-02DOI: 10.1080/23144599.2019.1675287
Z. S. Ulhaq
ABSTRACT Oestradiol (E2) is known as a female reproductive hormone with pleiotropic effects on the cardiovascular system. Local E2 biosynthesis such as in the brain and myocardial cells have important physiological and pathophysiological roles. E2 production is catalysed by aromatase (Aro) enzyme. In teleost, two Aro isoforms are distinctly expressed in the ovary and brain. In this study, the role of brain Aro (AroB) in modulating cardiovascular system is investigated. AroB MO-mediated knockdown decreased ventricular functions. Moreover, embryos injected with AroB MO displays a sign in developing heart failure. All the effects caused by AroB MO were partially reversed by exposure to E2. Taken together, this study demonstrates the role of AroB in modulating normal cardiovascular function in zebrafish embryos.
{"title":"Brain aromatase modulates cardiac functions in embryonic zebrafish","authors":"Z. S. Ulhaq","doi":"10.1080/23144599.2019.1675287","DOIUrl":"https://doi.org/10.1080/23144599.2019.1675287","url":null,"abstract":"ABSTRACT Oestradiol (E2) is known as a female reproductive hormone with pleiotropic effects on the cardiovascular system. Local E2 biosynthesis such as in the brain and myocardial cells have important physiological and pathophysiological roles. E2 production is catalysed by aromatase (Aro) enzyme. In teleost, two Aro isoforms are distinctly expressed in the ovary and brain. In this study, the role of brain Aro (AroB) in modulating cardiovascular system is investigated. AroB MO-mediated knockdown decreased ventricular functions. Moreover, embryos injected with AroB MO displays a sign in developing heart failure. All the effects caused by AroB MO were partially reversed by exposure to E2. Taken together, this study demonstrates the role of AroB in modulating normal cardiovascular function in zebrafish embryos.","PeriodicalId":45744,"journal":{"name":"International Journal of Veterinary Science and Medicine","volume":"28 1","pages":"31 - 34"},"PeriodicalIF":2.2,"publicationDate":"2019-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82568143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}