Journal of Oral Biosciences最新文献_第4页

Histochemical assessment of the femora of spontaneously diabetic torii-leprfa (SDT-fa/fa) rats that mimic type II diabetes 模拟II型糖尿病的自发性糖尿病Torii-Leprfa （SDT-fa/fa）大鼠股骨的组织化学评价

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-12-18 DOI: 10.1016/j.job.2024.100602

Qi Yao , Kanako Tsuboi , Hiromi Hongo , Mako Sakakibara , Tomomaya Yamamoto , Mai Haraguchi-Kitakamae , Hotaka Ishizu , Xuanyu Liu , Yan Shi , Weisong Li , Jiaxin Cui , Tomohiro Shimizu , Norio Amizuka , Atsuro Yokoyama , Tomoka Hasegawa , Kiwamu Sakaguchi

Objective

To elucidate the mechanisms underlying diabetic osteoporosis, we conducted a comprehensive histological examination of the femora of Spontaneously Diabetic Torii-Lepr^fa (SDT-fa/fa) rats, an established model of obesity-related type 2 diabetes.

Materials and methods

Femora from 12 30-week-old male SDT-fa/fa rats and age-matched Sprague–Dawley (SD) rats (controls) were used for detailed histochemical analyses, including tartrate-resistant acid phosphatase (TRAP), cathepsin K, tissue nonspecific alkaline phosphatase (ALP), phosphoethanolamine/phosphocholine phosphatase 1 (PHOSPHO1), dentin matrix protein 1 (DMP-1), matrix extracellular phosphoglycoprotein (MEPE), sclerostin, osteocalcin staining, silver impregnation, von Kossa staining, and micro-computed tomography (CT).

Results

Micro-CT and hematoxylin-eosin staining demonstrated significantly reduced trabecular bone volume in the femoral metaphyses of SDT-fa/fa rats. Although the number of TRAP-positive osteoclasts per bone surface remained comparable between both groups, SDT-fa/fa rats exhibited reduced areas of ALP-positive and PHOSPHO1-reactive mature osteoblasts/BS. Silver impregnation revealed a well-organized osteocytic lacunar–canalicular system in both groups. However, immunostaining identified aberrant DMP-1 and MEPE expression localized predominantly in the lacunae in SDT-fa/fa rats and in the lacunae and canaliculi of SD rats. Additionally, intense osteocalcin and sclerostin immunoreactivity was detected in osteocytes, along with a higher proportion of osteocalcin-positive osteocytes in SDT-fa/fa rats, distinguishing them from controls.

Conclusion

SDT-fa/fa rats displayed a significant decline in osteoblastic function and distinctive distribution patterns of osteocyte-derived peptides, suggesting that this diabetic model may manifest alterations in osteoblastic activity and the osteocytic lacunar-canalicular network.

目的：为了阐明糖尿病性骨质疏松的机制，我们对建立肥胖相关2型糖尿病模型的SDT-fa/fa大鼠进行了全面的股骨组织学检查。材料和方法：采用12只30周龄雄性SDT-fa/fa大鼠和年龄匹配的SD大鼠（对照）股骨进行详细的组织化学分析，包括抗酒石酸酸性磷酸酶（TRAP）、组织蛋白酶K、碱性磷酸酶（ALP）、磷酸乙醇胺/磷酸胆碱磷酸酶1 （PHOSPHO1）、牙本质基质蛋白(DMP)-1、基质细胞外磷酸糖蛋白（MEPE）、硬化蛋白、骨钙素染色、银浸渍、von Kossa染色和显微计算机断层扫描（CT）。结果：显微ct和苏木精-伊红染色显示，SDT-fa/fa大鼠股骨干骨小梁体积明显减少。尽管两组之间每个骨表面的trap阳性破骨细胞数量保持相当，但SDT-fa/fa大鼠表现出alp阳性和phospho1反应的成熟成骨细胞/BS面积减少。银浸渍显示两组均有组织良好的骨细胞腔管系统。然而，免疫染色发现DMP-1和MEPE的异常表达主要局限于SDT-fa/fa大鼠的腔隙和SD大鼠的腔隙和小管。此外，在骨细胞中检测到强烈的骨钙素和硬化蛋白免疫反应性，同时在SDT-fa/fa大鼠中检测到更高比例的骨钙素阳性骨细胞，将其与对照组区分开来。结论：SDT-fa/fa大鼠成骨功能明显下降，骨细胞衍生肽分布模式不同，提示该糖尿病模型可能出现成骨细胞活性和骨细胞腔隙网络的改变。

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引用次数: 0

Effect of anterior disc displacement and estrogen deficiency on rabbit mandibular condyle 椎间盘前移和雌激素缺乏对家兔下颌髁状突的影响

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-12-14 DOI: 10.1016/j.job.2024.100599

Takuya Iwasaki , Namiaki Takahara , Vu Viet Duc , Nobuyoshi Tomomatsu , Makoto J. Tabata , Tetsuya Yoda

Objective

In this study, we aimed to establish an experimental model of idiopathic condylar resorption by combining surgically induced anterior disc displacement and estrogen deficiency in growing rabbits. This study aimed to investigate the individual and combined effects of these factors on condylar resorption.

Materials and methods

Seventeen female Japanese White rabbits were divided into four groups: control, ovariectomy, anterior disc displacement surgery, and combination of ovariectomy and anterior disc displacement surgery. Micro-computed tomography and histological evaluations were performed to analyze changes in the trabecular bone structure and cartilage thickness of the condyle.

Results

The combined group exhibited the most significant changes in trabecular bone parameters, including the lowest bone volume per tissue volume and trabecular number and the highest trabecular separation and spacing. Histologically, the fibrous layer thinned and the hypertrophic layer thickened in the anterior and central parts of the condyle in the anterior disc displacement and combined groups. The Modified Mankin Score indicated the highest level of degenerative change in the combined group.

Conclusion

The combination of surgically induced anterior disc displacement and estrogen deficiency in growing rabbits effectively modeled idiopathic condylar resorption, demonstrating the synergistic impact of disc displacement and estrogen deficiency on condylar resorption. This model provides valuable insight into the pathogenesis of idiopathic condylar resorption.

研究目的本研究旨在通过手术诱导椎间盘前移和雌激素缺乏，在生长期家兔中建立特发性髁突吸收的实验模型。本研究旨在探讨这些因素对髁突吸收的单独和联合影响：将 17 只雌性日本白兔分为四组：对照组、卵巢切除组、椎间盘前移位手术组和卵巢切除与椎间盘前移位手术联合组。对髁状突的骨小梁结构和软骨厚度的变化进行了显微计算机断层扫描和组织学评估：结果：联合组的骨小梁参数变化最明显，包括单位组织体积骨量和骨小梁数量最低，骨小梁分离度和间距最高。从组织学角度看，在椎间盘前移位组和联合组中，髁突前部和中部的纤维层变薄，增生层增厚。改良 Mankin 评分显示，联合组的退行性改变程度最高：结论：通过手术诱导椎间盘前移位和雌激素缺乏对生长兔进行联合治疗，可有效模拟特发性髁突吸收，证明椎间盘前移位和雌激素缺乏对髁突吸收有协同作用。该模型为了解特发性髁突吸收的发病机制提供了宝贵的信息。

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引用次数: 0

Measurement of advanced glycation end products (AGEs) accumulated in dentin collagen 牙本质胶原中积累的晚期糖基化终产物（AGEs）的测量。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-12-12 DOI: 10.1016/j.job.2024.100600

Ruri Asami, Takuya Sato, Koji Sakiyama

Objective

In the Maillard reaction, reducing sugars bind to proteins in a non-enzymatic manner, and the final products are collectively named, advanced glycation end products (AGEs). AGEs accumulate in the human body with age and cause various disorders in tissues and cells. In this study, pentosidine, an AGE that can be stably measured, was used to investigate the differences in pentosidine accumulation within dentin, and whether the results can indicate age.

Methods

High-performance liquid chromatography was used to measure pentosidine, an AGE that accumulates in dentin collagen, in extracted teeth with no caries and a clear history.

Results

The dentin pentosidine concentration increased with age, with a higher pentosidine concentration observed at the enamel-dentin junction aspect than at the pulp cavity aspect. A close correlation was observed between the dentin pentosidine level and age.

Conclusions

These findings suggest the utility of measuring accumulation of AGEs in dentin collagen as an indicator of age.

目的：在美拉德反应中，还原糖以非酶的方式与蛋白质结合，最终产物被统称为晚期糖基化终产物（age）。AGEs在人体内随着年龄的增长而积累，并引起组织和细胞的各种紊乱。本研究利用一种可以稳定测量的年龄——戊苷，研究了牙本质内戊苷积累的差异，以及结果是否可以指示年龄。方法：采用高效液相色谱法测定无龋病史的拔牙牙本质胶原中积累的AGE戊苷。结果：牙本质戊苷浓度随年龄增长而增加，牙釉质-牙本质交界处的戊苷浓度高于牙髓腔。牙本质戊苷水平与年龄密切相关。结论：这些发现表明测量牙本质胶原中AGEs积累作为年龄指标的效用。

引用次数: 0

The potential anti-inflammatory effect of hyaluronic acid gel alone or in combination with grapefruit seed extract on induced periodontitis in mandibular molars of Wistar rats 透明质酸凝胶单独或联合葡萄柚籽提取物对Wistar大鼠下颌磨牙诱导牙周炎的潜在抗炎作用。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-12-12 DOI: 10.1016/j.job.2024.100598

Sana Mostafa , Aiah A. El-Rashidy , Mohamed Talaat Elbehwashy , Manar A. Abdul-Aziz , Nermeen AbuBakr

Objectives

Antimicrobial agents have been used in conjunction with conventional chemomechanical therapy to improve the treatment outcomes of periodontitis. This study aimed to evaluate the ameliorating effect of topical application of hyaluronic acid (HA) with or without grapefruit seed extract (GFSE) (5, 10, and 15 wt %) in induced periodontitis in rats.

Methods

Surgical alveolar bone defects were created in 30 adult male Wistar rats, followed by the introduction of a ligature impregnated with Escherichia coli lipopolysaccharide for four weeks to induce periodontitis. Rats were distributed into five groups (n = 6); an untreated periodontitis group and four treated groups in which gel (HA±GFSE) was injected into the sulcus once weekly for two weeks. All rats were euthanized six weeks after starting the experiment, and the mandibles were prepared for histopathological and histomorphometric analyses. Enzyme-linked immunosorbent assay was used for measuring tissue levels of tumor necrosis factor-alpha (TNF-α), transforming growth factor-β1 (TGF-β1), and paraoxonase-1 (PON-1) enzyme.

Results

HA enhanced new bone formation at defect margins, thereby diminishing defect width. This effect significantly increased as HA was combined with GFSE in a dose-dependent manner. Moreover, the HA and GFSE mixture suppressed the tissue levels of TNF-α and TGF-β1, thereby increasing PON-1.

Conclusion

The HA and GFSE mixtures exhibited synergistic therapeutic potential for the treatment of chronic periodontitis in a dose-dependent manner.

目的：抗菌药物与常规化学机械治疗相结合，以改善牙周炎的治疗效果。本研究旨在评估局部应用透明质酸（HA）加或不加葡萄柚籽提取物（GFSE）（5%、10%和15% wt. %）对大鼠诱导牙周炎的改善作用。方法：用30只成年雄性Wistar大鼠造牙槽骨缺损，用大肠杆菌脂多糖浸渍结扎法诱导牙周炎4周。将大鼠分为5组（n = 6）；牙周炎未治疗组和四个治疗组，每周向龈沟注射凝胶（HA +/- GFSE）一次，持续两周。实验开始6周后，对所有大鼠实施安乐死，制备下颌骨进行组织病理学和组织形态学分析。采用酶联免疫吸附法测定组织中肿瘤坏死因子-α (TNF-α)、转化生长因子-β1 （TGF-β1）、对氧磷酶-1 （PON-1）酶水平。结果：透明质酸促进缺损边缘的新骨形成，从而减小缺损宽度。当HA与GFSE以剂量依赖的方式联合使用时，这种效果显著增加。此外，HA和GFSE混合物抑制TNF-α和TGF-β1的组织水平，从而增加PON-1。结论：透明质酸与GFSE合剂治疗慢性牙周炎具有一定的剂量依赖性。

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引用次数: 0

Role of trigeminal ganglion satellite glial cells in masseter muscle pain hypersensitivity 三叉神经节卫星胶质细胞在咬肌疼痛超敏反应中的作用。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-12-11 DOI: 10.1016/j.job.2024.100596

Sho Sawada , Suzuro Hitomi , Yoshinori Hayashi , Hirotaka Shinozuka , Yoshiyuki Yonehara , Koichi Iwata , Masamichi Shinoda

Objectives

The underlying mechanism of masseter muscle pain hypersensitivity by sustained masseter muscle contraction (SMMC) is not well understood. This study aimed to examine whether the activation of satellite glial cells in the trigeminal ganglion (TG) contributes to masseter muscle pain hypersensitivity induced by SMMC.

Methods

Electrodes were placed on the masseter muscle fascia of rats to induce strong contractions, by daily electrical stimulation. Pain sensitivity in the masseter muscle was measured and the activation level of satellite glial cells in the TG was examined. The localization of P2Y₁₂ and the effects of P2Y₁₂ receptor inhibition on SMMC-induced pain hypersensitivity were evaluated. The amount of tumor necrosis factor alpha (TNF-α) and TNF-α receptor localization were determined in the TG.

Results

SMMC induced masseter muscle pain hypersensitivity and activation of satellite glial cells. P2Y₁₂ receptors were expressed in satellite glial cells and masseter muscle pain hypersensitivity was suppressed by intra-TG P2Y₁₂ receptor antagonism. TG neurons innervating the sustained-contracted masseter muscle expressed TNF-α receptor and SMMC increased TNF-α levels in TG.

Conclusion

SMMC-induced activation of satellite glial cells though the P2Y₁₂ receptor signaling may contribute to masseter muscle pain hypersensitivity via the TNF-α signaling pathway.

目的：咬肌持续收缩（SMMC）引起咬肌疼痛超敏反应的潜在机制尚不清楚。本研究旨在探讨三叉神经节（TG）卫星胶质细胞的激活是否与SMMC诱导的咬肌疼痛超敏反应有关。方法：在大鼠咬肌筋膜上放置电极，通过每日电刺激引起咬肌强烈收缩。测定大鼠咬肌的疼痛敏感性，观察大鼠TG中卫星神经胶质细胞的活化水平。评估P2Y12的定位及P2Y12受体抑制对smmc诱导的疼痛超敏反应的影响。测定TG中肿瘤坏死因子α (TNF-α)的含量及TNF-α受体的定位。结果：SMMC诱导咬肌疼痛超敏反应和卫星神经胶质细胞活化。P2Y12受体在卫星胶质细胞中表达，tg内P2Y12受体拮抗剂抑制咬肌疼痛超敏反应。支配咬肌持续收缩的TG神经元表达TNF-α受体，SMMC升高TG中TNF-α水平。结论：smmc通过P2Y12受体信号通路激活卫星神经胶质细胞，可能通过TNF-α信号通路参与咬肌疼痛超敏反应。

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引用次数: 0

The cortical areas processing periodontal ligament nociception in mice 处理小鼠牙周韧带痛觉的皮层区域。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-12-10 DOI: 10.1016/j.job.2024.100597

Risako Okuma , Shutaro Kobayashi , Satomi Kobayashi , Yoshinori Arai , Naoyuki Matsumoto , Mitsuru Motoyoshi , Masayuki Kobayashi , Satoshi Fujita

Objectives

Toothaches are often poorly localized. Although periodontal pain is better localized, it can spread to other areas. Ultimately, the cerebral cortex processes nociception, with somatotopic organization possibly playing a role in localizing the origin. However, the exact cortical area in the periodontal ligament (PDL) remains unclear.

Methods

This study examined cortical responses to electrical stimulation of the molar PDL in anesthetized male mice using in vivo optical imaging with a voltage-sensitive dye, autofluorescent flavin fluorescence, and immunohistochemistry for c-Fos protein expression.

Results

On optical imaging, cortical responses to the stimulation of the ipsilateral and contralateral PDL of the upper and lower teeth were observed in the primary somatosensory cortex (S1) and area from the insular cortex (IC) to the ventral edge of the secondary somatosensory cortex (S2), defined as the area caudal to the middle cerebral artery (C-area). Responses in S1 were faint and unstable, but were consistent in the C-area. The initial response locations were similar regardless of which PDL was stimulated, and the activated areas in the C-area almost overlapped. Three-dimensional construction of c-Fos-immunopositive cells responding to upper or lower PDL stimulation revealed bilateral distribution in the cingulate gyrus, secondary auditory cortex, temporal association cortex, ectorhinal cortex, and IC, but not in the S1 and S2.

Conclusion

These results suggest that the somatotopic organization of the S1, S2, and IC cannot explain the localization of PDL nociception. The predominance of responses in the contralateral IC may provide clues for identifying the laterality.

目标：牙痛的局部性通常很差。虽然牙周疼痛的局部性较好，但也会扩散到其他部位。最终，大脑皮层会处理痛觉，躯体位点组织可能在定位痛觉起源方面发挥作用。然而，牙周韧带（PDL）的确切皮质区域仍不清楚：本研究使用电压敏感染料、自发荧光黄素荧光和c-Fos蛋白表达的免疫组织化学方法对麻醉雄性小鼠的磨牙PDL电刺激皮质反应进行了检测：通过光学成像，在初级躯体感觉皮层（S1）和从岛叶皮层（IC）到次级躯体感觉皮层（S2）腹侧边缘的区域（定义为大脑中动脉尾部的区域（C区））观察到皮层对刺激同侧和对侧上下牙齿PDL的反应。S1 中的反应微弱且不稳定，但在 C 区中反应一致。无论刺激哪个 PDL，最初的反应位置都相似，而且 C 区的激活区域几乎重叠。对上部或下部 PDL 刺激产生反应的 c-Fos 免疫阳性细胞的三维结构显示，这些细胞双侧分布在扣带回、次级听觉皮层、颞联想皮层、外侧皮层和 IC，但不在 S1 和 S2：这些结果表明，S1、S2 和 IC 的体位组织不能解释 PDL 痛觉的定位。对侧 IC 的主要反应可能为确定侧位提供了线索。

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引用次数: 0

Mirror-polished ceria-stabilized zirconia/alumina nanocomposite enhances gingival junctional epithelial cell adhesion 镜面抛光氧化铈稳定氧化锆/氧化铝纳米复合材料增强牙龈结上皮细胞粘附。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-12-09 DOI: 10.1016/j.job.2024.100593

Shoma Yamamori , Eri Urano-Morisawa , Ayako Mochizuki , Ryo Aizawa , Fuminori Iwasa , Matsuo Yamamoto , Kazuyoshi Baba

Objectives

The aim of this study was to determine the optimal surface roughness (Ra) of ceria-stabilized zirconia/alumina nanocomposite (Ce-TZP/Al₂O₃) implants for mouse gingival junctional epithelial cell (JE-1) adhesion and soft tissue sealing in vitro.

Methods

Titanium and Ce-TZP/Al₂O₃ disks were prepared, mechanically polished (M), and mirror-polished (Mr). The surface morphology of each disk was evaluated, and the Ra was measured using scanning electron microscopy and atomic force microscopy. JE-1 cells were cultured on each disk, and cell proliferation was assessed by measuring the absorbance using the MTS assay. We also analyzed the expression of the adhesion proteins Laminin-5, Integrin β4, and Cadherin-1 using immunostaining and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The adhesion strength of the JE-1 cells to each disk was measured using a shaking stimulation test.

Results

M disks had rough surfaces, whereas Mr disks had smooth morphologies. JE-1 cell proliferation was proportional to the culture time, and the Mr disks showed higher values than the M disk. Immunofluorescence and qRT-PCR showed that expression of Laminin-5 and Integrin β4 was higher with Mr disks than with M on the Ce-TZP/Al₂O₃ disks. The oscillatory stimulation test also showed that the adhesive strength of JE-1 cells was significantly higher with Mr than with M on the Ce-TZP/Al₂O₃ disks.

Conclusions

Mirror polishing of Ce-TZP/Al₂O₃ disks enhances epithelial cell proliferation and adhesion more than mechanical polishing. These findings have implications for the optimization of implant surface characteristics to improve epithelial sealing.

目的：研究铈稳定氧化锆/氧化铝纳米复合材料（Ce-TZP/Al₂O₃）种植体的最佳表面粗糙度（Ra），用于小鼠牙龈结上皮细胞（JE-1）的体外粘附和软组织密封。方法：制备钛和Ce-TZP/Al₂O₃圆盘，机械抛光(M)和镜面抛光（Mr）。利用扫描电镜和原子力显微镜对各圆盘的表面形貌进行了评价，并对Ra进行了测定。在每个培养皿上培养JE-1细胞，MTS法测定吸光度，评估细胞增殖情况。我们还利用免疫染色和定量逆转录聚合酶链反应（qRT-PCR）分析了粘附蛋白Laminin-5、Integrin β4和Cadherin-1的表达。采用振动刺激试验测量JE-1细胞与各圆盘的粘附强度。结果：M盘表面粗糙，Mr盘表面光滑。JE-1细胞增殖与培养时间成正比，Mr盘高于M盘。免疫荧光和qRT-PCR显示Mr圆盘上Laminin-5和Integrin β4的表达高于M在Ce-TZP/Al₂O₃圆盘上的表达。振荡刺激实验还表明，Mr对JE-1细胞在Ce-TZP/Al₂O₃磁盘上的粘附强度显著高于M对JE-1细胞的粘附强度。结论：镜面抛光Ce-TZP/Al₂O₃圆盘比机械抛光更能促进上皮细胞的增殖和粘附。这些发现对优化种植体表面特征以改善上皮密封具有重要意义。

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引用次数: 0

Assessment of biofilm formation on ceramic, metal, and plastic brackets in orthodontic materials by new method using renG-expressing Streptococcus mutans 利用表达reng的变形链球菌的新方法评估正畸材料中陶瓷、金属和塑料托槽的生物膜形成。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-12-06 DOI: 10.1016/j.job.2024.100594

Hiroyuki Kato , Hiroko Yoshida , Masanori Saito , Tomomi Hashizume-Takizawa , Shinichi Negishi , Hidenobu Senpuku

Objective

Oral biofilm has a high acid-producing capacity, increases the risk of enamel demineralization around brackets, and has been identified as a problem in orthodontic treatment. Here, we assessed the risk of biofilm formation by Streptococcus mutans, which is associated with the development of white spot lesions (WSL) on tooth surfaces, using multibracket devices.

Methods

Various types of brackets were used for the biofilm formation assay with S. mutans coated with human saliva, immersed in renG-expressing S. mutans UA159 (strain with the luciferase gene inserted), and incubated overnight at 37 °C under aerobic conditions containing 5% CO₂. The biofilm was washed twice with phosphate-buffered saline (PBS), and 200 μL of luciferin dissolved in PBS was added to each well. The mixture was light shielded and allowed to react for 20 min. Luminescence was measured as the amount of biofilm formed by live cells on the bracket surfaces using an optical emission spectrophotometer.

Results

Biofilm formation was greater in plastic brackets than in ceramic and metal brackets in a number-dependent manner. However, biofilm formation was inhibited as the plastic bracket was coated with saliva.

Conclusion

For preventive treatments of WSL onset during orthodontic treatment, orthodontists should carefully select and customize brackets based on patient needs, goals, and biomechanical principles. This study developed a new measurement method using renG-expressing S. mutans UA159 to accurately assess active biofilm formation on bracket surfaces.

目的：口腔生物膜具有较高的产酸能力，增加了托槽周围牙釉质脱矿的风险，已被确定为正畸治疗中的一个问题。在这里，我们评估了变形链球菌形成生物膜的风险，这与牙齿表面白斑病变（WSL）的发展有关，使用多支架装置。方法：将人唾液包被变形链球菌，用不同类型的支架进行生物膜形成实验，浸入表达reng的变形链球菌UA159（插入荧光素酶基因的菌株）中，在含5% CO2的有氧条件下，37℃孵育过夜。用磷酸盐缓冲盐水（PBS）洗涤生物膜2次，每孔加入溶解在PBS中的荧光素200 μL。将混合物遮光并反应20分钟。使用光学发射分光光度计以活细胞在支架表面形成的生物膜的量来测量发光。结果：塑料托槽的生物膜形成量大于陶瓷托槽和金属托槽。然而，当塑料支架被唾液包裹时，生物膜的形成受到抑制。结论：为了预防正畸治疗中WSL的发生，正畸医师应根据患者的需要、目标和生物力学原理精心选择和定制托槽。本研究开发了一种新的测量方法，使用表达reng的变形链球菌UA159来准确评估支架表面活性生物膜的形成。

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引用次数: 0

Functional morphology of myoepithelial cells in the rat salivary glands: A review 大鼠唾液腺肌上皮细胞的功能形态学研究进展。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-11-29 DOI: 10.1016/j.job.2024.100592

Osamu Amano , Go Onozawa , Fuyoko Taira , Yoshihiro Kawabe , Kenichi Mizobe , Miyuki Toda , Arata Nagasaka , Yasuhiko Bando , Koji Sakiyama

Background

The acini, the secretory endpieces of the salivary glands, are composed of serous and/or mucous acinar cells and surrounded by myoepithelial cells. Myoepithelial cells are multipolar, stellate cells with long processes encircling the acini and intercalated ducts. These cells contract to facilitate salivary secretion and transport.

Highlight

In rat major salivary gland acini, the morphology of myoepithelial cells varies across glands: parotid glands lack myoepithelial cells, submandibular glands contain "slender"-shaped cells, and sublingual glands contain "macho"-shaped cells. These morphological variations are thought to depend on the salivary viscosity. Myoepithelial cells in the intercalated ducts exhibit minimal variation across the major salivary glands, with processes oriented parallel to the duct axis. These cells are covered by a thin collagen layer and small fibroblasts, collectively termed "the peri-intercalated duct sheath." Ebner's glands, located beneath the circumvallate and foliate papillae and containing numerous taste buds, develop myoepithelial cells in both the acini and intercalated ducts to facilitate vigorous saliva secretion, enhancing gustatory sensitivity.

Conclusions

The morphology of myoepithelial cells is influenced by their functional roles under different anatomical, physiological, and pathological conditions. Increased thickness and branching occur to adapt to salivary viscosity and/or enhance secretion. In the intercalated ducts, myoepithelial cells support salivary transport with the aid of the surrounding collagen layer and fibroblasts in "the peri-intercalated duct sheath".

背景：腺泡是唾液腺的分泌末端，由浆液和/或粘液腺泡细胞组成，并被肌上皮细胞包围。肌上皮细胞是多极的星状细胞，长突环绕着腺泡和插管。这些细胞收缩以促进唾液分泌和运输。重点提示：大鼠大唾液腺腺腺腺腺肌上皮细胞形态在不同腺体间存在差异：腮腺缺乏肌上皮细胞，下颌骨腺含有“细长”型细胞，舌下腺含有“大男子”型细胞。这些形态变化被认为取决于唾液的黏度。嵌入导管中的肌上皮细胞在主要唾液腺中表现出最小的变化，其突起平行于导管轴。这些细胞被一层薄薄的胶原蛋白层和小的成纤维细胞所覆盖，统称为“周插间管鞘”。艾氏腺位于周状和叶状乳头下方，含有大量的味蕾，在腺泡和插管中发育肌上皮细胞，促进唾液分泌，增强味觉敏感性。结论：在不同解剖、生理和病理条件下，肌上皮细胞的形态受其功能作用的影响。增加的厚度和分支发生适应唾液粘度和/或促进分泌。在插间管中，肌上皮细胞在周围胶原层和“插间管周围鞘”的成纤维细胞的帮助下支持唾液运输。

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引用次数: 0

Expression and localization of adiponectin in myoepithelial cells in sublingual glands of normal and diabetic rats 脂联素在正常和糖尿病大鼠舌下腺肌上皮细胞中的表达和定位。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-11-28 DOI: 10.1016/j.job.2024.100590

Genki Miyake , Arata Nagasaka , Yasuhiko Bando , Koji Sakiyama , Shoichi Iseki , Hideaki Sakashita , Osamu Amano

Objectives

Adiponectin is a hormone produced by adipocytes with anti-atherosclerotic and anti-diabetic properties. We previously discovered that adiponectin is specifically localized in the myoepithelial cells of rat sublingual glands. This study aims to investigate the localization of adiponectin and its receptors, AdipoR1 and AdipoR2, in adult rats, postnatally developing rats, and diabetic model rats.

Methods

We examined the localization and expression of adiponectin and its receptors by immunohistochemistry and RT-PCR in the sublingual glands of adult rats and in two diabetic rat models: Streptozotocin (STZ)-treated rats for type 1 diabetes and GK rats for type 2 diabetes.

Results

In rat sublingual glands, adiponectin was localized in the cytoplasm of myoepithelial cells, while AdipoR1 and AdipoR2 were localized in the basolateral membrane of mucous acinar cells. In GK rats, there was a significant decrease in the immunoreactivity and mRNA levels of adiponectin, while both AdipoR1 and AdipoR2 expression levels were upregulated. In STZ-treated rats, both adiponectin and its receptors showed reduced expression.

Conclusions

Adiponectin acts as a paracrine factor in sublingual myoepithelial cells, influencing salivary secretion through upregulated receptors in acinar cells, particularly in type 2 diabetes. This process is associated with a reduction in myoepithelial adiponectin levels.

目的：脂联素是一种由脂肪细胞产生的具有抗动脉粥样硬化和抗糖尿病特性的激素。我们先前发现脂联素特异性地定位于大鼠舌下腺的肌上皮细胞。本研究旨在探讨脂联素及其受体AdipoR1和AdipoR2在成年大鼠、出生后发育大鼠和糖尿病模型大鼠中的定位。方法：采用免疫组织化学和RT-PCR方法检测脂联素及其受体在成年大鼠和两种糖尿病大鼠模型中的定位和表达：链脲佐菌素（STZ）治疗的1型糖尿病大鼠和GK治疗的2型糖尿病大鼠。结果：在大鼠舌下腺中，脂联素定位于肌上皮细胞的细胞质中，而AdipoR1和AdipoR2定位于粘液腺泡细胞的基底外膜中。GK大鼠免疫反应性和脂联素mRNA水平显著降低，AdipoR1和AdipoR2表达水平上调。在stz处理的大鼠中，脂联素及其受体的表达均降低。结论：脂联素在舌下肌上皮细胞中作为旁分泌因子，通过腺泡细胞中受体的上调影响唾液分泌，特别是在2型糖尿病中。这一过程与肌上皮脂联素水平的降低有关。

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引用次数: 0