Journal of Oral Biosciences最新文献_第6页

Specific localization of fibroblasts at the intercalated duct in the major salivary glands of rats 成纤维细胞在大鼠主要唾液腺闰管处的特异性定位

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.02.004

Go Onozawa , Arata Nagasaka , Yasuhiko Bando , Koji Sakiyama , Nobuharu Yamamoto , Osamu Amano

Objectives

Immunohistochemical methods were employed to investigate the morphological heterogeneity and localization of fibroblasts associated with the function of major salivary glands in rats.

Methods

Histochemical and electron microscopic observations were made in rat parotid, submandibular, and sublingual glands and pancreas. Fibroblasts were immunostained using their specific marker, 47 kDa heat shock protein (Hsp47).

Results

Hsp47-immunopositive fibroblasts within the intralobular connective tissue exhibited a notably smaller size compared with the interlobular connective tissue. They were loosely distributed throughout the connective tissue. However, fibroblasts with elongated long processes were explicitly identified at the intercalated ducts in parotid, sublingual, and submandibular glands. Fibroblastic bodies and processes were tightly approximated with the basement membrane of the duct. Electron microscopy confirmed these findings, revealing a thin layer consisting of collagen fibers was found between the fibroblasts and the basement membrane. Double staining of Hsp47 and α-smooth muscle actin (αSMA) in parotid glands indicating that Hsp47-positive fibroblasts enveloped both the duct and αSMA-positive myoepithelial cells. Additionally, They projected long and thin processes longitudinally at the straight portion or circularly at the bifurcated portion of the duct. The three-dimensional reconstruction showed a frame-like structure of fibroblasts surrounding the intercalated duct with longitudinal myoepithelial cells. However, such specific localization of fibroblasts was not detected in the exocrine pancreas lacking myoepithelium.

Conclusions

Small fibroblasts with long processes connecting or overwrapping each other and thin collagen layers surround the intercalated ducts in rat major salivary glands, presumably contributing to protecting the ducts from salivary flow and myoepithelial contraction.

目的：采用免疫组化方法研究与大鼠主要唾液腺功能相关的成纤维细胞的形态异质性和定位：方法：对大鼠腮腺、颌下腺、舌下腺和胰腺进行组织化学和电子显微镜观察。使用成纤维细胞的特异性标记物 47 kDa 热休克蛋白（Hsp47）对其进行免疫染色：结果：与小叶间结缔组织相比，小叶内结缔组织中 Hsp47 免疫阳性成纤维细胞的体积明显较小。它们松散地分布在整个结缔组织中。不过，在腮腺、舌下腺和颌下腺的闰导管中，可以清晰地发现带有细长突起的成纤维细胞。成纤维细胞体和过程与导管基底膜紧密贴合。电子显微镜证实了这些发现，显示在成纤维细胞和基底膜之间有一层由胶原纤维组成的薄层。腮腺中 Hsp47 和 α 平滑肌肌动蛋白（αSMA）的双重染色表明，Hsp47 阳性的成纤维细胞同时包裹着导管和 αSMA 阳性的肌上皮细胞。此外，它们还在导管的直线部分纵向或分叉部分环形伸出细长的突起。三维重建显示，成纤维细胞与纵向肌上皮细胞组成的框架结构围绕着插管。然而，在缺乏肌上皮细胞的外分泌胰腺中却未检测到成纤维细胞的这种特异性定位：结论：大鼠大唾液腺的闰导管周围环绕着小的成纤维细胞，这些成纤维细胞具有相互连接或包裹的长过程和薄胶原层，可能有助于保护闰导管免受唾液流和肌上皮收缩的影响。

{"title":"Specific localization of fibroblasts at the intercalated duct in the major salivary glands of rats","authors":"Go Onozawa , Arata Nagasaka , Yasuhiko Bando , Koji Sakiyama , Nobuharu Yamamoto , Osamu Amano","doi":"10.1016/j.job.2024.02.004","DOIUrl":"10.1016/j.job.2024.02.004","url":null,"abstract":"<div><h3>Objectives</h3><p>Immunohistochemical methods were employed to investigate the morphological heterogeneity and localization of fibroblasts associated with the function of major salivary glands in rats.</p></div><div><h3>Methods</h3><p>Histochemical and electron microscopic observations were made in rat parotid, submandibular, and sublingual glands and pancreas. Fibroblasts were immunostained using their specific marker, 47 kDa heat shock protein (Hsp47).</p></div><div><h3>Results</h3><p>Hsp47-immunopositive fibroblasts within the intralobular connective tissue exhibited a notably smaller size compared with the interlobular connective tissue. They were loosely distributed throughout the connective tissue. However, fibroblasts with elongated long processes were explicitly identified at the intercalated ducts in parotid, sublingual, and submandibular glands. Fibroblastic bodies and processes were tightly approximated with the basement membrane of the duct. Electron microscopy confirmed these findings, revealing a thin layer consisting of collagen fibers was found between the fibroblasts and the basement membrane. Double staining of Hsp47 and α-smooth muscle actin (αSMA) in parotid glands indicating that Hsp47-positive fibroblasts enveloped both the duct and αSMA-positive myoepithelial cells. Additionally, They projected long and thin processes longitudinally at the straight portion or circularly at the bifurcated portion of the duct. The three-dimensional reconstruction showed a frame-like structure of fibroblasts surrounding the intercalated duct with longitudinal myoepithelial cells. However, such specific localization of fibroblasts was not detected in the exocrine pancreas lacking myoepithelium.</p></div><div><h3>Conclusions</h3><p>Small fibroblasts with long processes connecting or overwrapping each other and thin collagen layers surround the intercalated ducts in rat major salivary glands, presumably contributing to protecting the ducts from salivary flow and myoepithelial contraction.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 456-464"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abnormal bone regeneration induced by FK506 in medaka fin revealed by in vivo imaging 通过体内成像揭示 FK506 诱导的青鳉鱼鳍异常骨再生。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.02.007

Kai Otake , Yuki Azetsu , Masahiro Chatani , Akiko Karakawa , Satoko Nishida , Aiko Hirayama , Rina Kobayashi , Nobuhiro Sakai , Noriyuki Suzuki , Masamichi Takami

Objectives

Bone tissue in bony fish demonstrates a remarkable ability to regenerate, particularly evident following induction of extensive bone defects, such as fin amputation. This regenerative capacity has been reported to be promoted by the immunosuppressant FK506, yet its precise effects on bone cells during fin regeneration remains insufficiently elucidated. This study aims to investigate the effects of FK506 treatment on bone morphology, osteoblasts, and osteoclasts in the bony fin rays of osterix promoter-DsRed/TRAP promoter-EGFP double transgenic (Tg) medaka.

Methods

The caudal fin of double Tg medaka was amputated, followed by a 20-day treatment with FK506 (1.0 μg/ml) to observe its effects on fin regeneration. Additionally, the regenerated caudal fin area underwent evaluation using genetic analysis and cell proliferation assays.

Results

FK506 treatment significantly increased osterix-positive osteoblast formation, resulting in both a significantly longer fin length and fewer joints in the bony fin rays formed during fin regeneration. Notably, TRAP-positive osteoclast formation and bone resorption were observed to occur primarily during the latter stages of fin regeneration. Furthermore, while the expression levels of osteoblast-related genes in the regenerated area remained unchanged following FK506 treatment, a heightened cell proliferation was observed at the tip of the fin.

Conclusions

Our findings suggest that treatment with FK506 promotes bone regeneration by increasing the number of osteoblasts in the amputated area of the fin. However, long-term treatment disrupts regular bone metabolism by inducing abnormal osteoclast formation.

目的：多骨鱼的骨组织具有惊人的再生能力，在诱导大面积骨缺损（如鳍截肢）后尤为明显。据报道，免疫抑制剂 FK506 可促进这种再生能力，但其在鱼鳍再生过程中对骨细胞的确切影响仍未得到充分阐明。本研究旨在探讨 FK506 处理对 osterix 启动子-DsRed/TRAP 启动子-EGFP 双转基因（Tg）青鳉骨鳍射线中骨形态、成骨细胞和破骨细胞的影响：方法：截去双Tg青鳉的尾鳍，然后用FK506（1.0 μg/ml）处理20天，观察其对鳍再生的影响。此外，还利用遗传分析和细胞增殖试验对再生的尾鳍面积进行了评估：结果：FK506能明显增加sterix阳性成骨细胞的形成，从而使鳍的长度明显延长，而且在鳍再生过程中形成的骨性鳍条的关节更少。值得注意的是，TRAP 阳性破骨细胞的形成和骨吸收主要发生在鳍再生的后期阶段。此外，虽然 FK506 治疗后再生区域成骨细胞相关基因的表达水平保持不变，但在鳍的顶端观察到细胞增殖增加：我们的研究结果表明，使用 FK506 治疗可通过增加鳍截肢区域成骨细胞的数量来促进骨再生。结论：我们的研究结果表明，使用 FK506 治疗可通过增加鳍截肢部位成骨细胞的数量来促进骨再生，但长期治疗会通过诱导异常破骨细胞的形成来破坏正常的骨代谢。

{"title":"Abnormal bone regeneration induced by FK506 in medaka fin revealed by in vivo imaging","authors":"Kai Otake , Yuki Azetsu , Masahiro Chatani , Akiko Karakawa , Satoko Nishida , Aiko Hirayama , Rina Kobayashi , Nobuhiro Sakai , Noriyuki Suzuki , Masamichi Takami","doi":"10.1016/j.job.2024.02.007","DOIUrl":"10.1016/j.job.2024.02.007","url":null,"abstract":"<div><h3>Objectives</h3><p>Bone tissue in bony fish demonstrates a remarkable ability to regenerate, particularly evident following induction of extensive bone defects, such as fin amputation. This regenerative capacity has been reported to be promoted by the immunosuppressant FK506, yet its precise effects on bone cells during fin regeneration remains insufficiently elucidated. This study aims to investigate the effects of FK506 treatment on bone morphology, osteoblasts, and osteoclasts in the bony fin rays of <em>osterix</em> promoter-DsRed/<em>TRAP</em> promoter-EGFP double transgenic (Tg) medaka.</p></div><div><h3>Methods</h3><p>The caudal fin of double Tg medaka was amputated, followed by a 20-day treatment with FK506 (1.0 μg/ml) to observe its effects on fin regeneration. Additionally, the regenerated caudal fin area underwent evaluation using genetic analysis and cell proliferation assays.</p></div><div><h3>Results</h3><p>FK506 treatment significantly increased <em>osterix</em>-positive osteoblast formation, resulting in both a significantly longer fin length and fewer joints in the bony fin rays formed during fin regeneration. Notably, <em>TRAP</em>-positive osteoclast formation and bone resorption were observed to occur primarily during the latter stages of fin regeneration. Furthermore, while the expression levels of osteoblast-related genes in the regenerated area remained unchanged following FK506 treatment, a heightened cell proliferation was observed at the tip of the fin.</p></div><div><h3>Conclusions</h3><p>Our findings suggest that treatment with FK506 promotes bone regeneration by increasing the number of osteoblasts in the amputated area of the fin. However, long-term treatment disrupts regular bone metabolism by inducing abnormal osteoclast formation.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 381-390"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139997820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-cancer agent piperlongumine is an inhibitor of transient receptor potential melastatin 7 channel in oral squamous cell carcinoma 抗癌剂哌隆罗明是口腔鳞状细胞癌中瞬时受体电位美拉德7通道的抑制剂。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.03.002

Tao Su , Yi-hui Chen , Kan-kui Wu , Xiao-hong Xu

Objectives

To elucidate the association between the anticancer activities of piperlongumine (PL) and its potential target, transient receptor potential melastatin 7 channel (TRPM7), in oral squamous cell carcinoma (OSCC).

Methods

The expression levels and electrical characteristics of TRPM7 as well as cell viability in response to various PL treatments were investigated in the OSCC cell line Cal27.

Results

PL treatment resulted in a concentration- and time-dependent reduction in TRPM7 mRNA and protein expression in Cal27 cells. Furthermore, PL treatment inhibited TRPM7-like rectifying currents in Cal27 cells; however, this inhibition was less effective than that of the TRPM7 antagonist waixenicin A. Rapid perfusion and washout experiments revealed an immediate inhibitory effect of PL on TRPM7-like currents. The antagonistic effect of PL occurred within 1 min and was not completely reversed following washout. Notably, the extracellular Ca²⁺ concentration still influenced PL-induced changes in the TRPM7-like current, indicating that PL can directly but gently antagonize the TRPM7 channel. Functional changes in TRPM7 correlated with the observed antiproliferative and cytotoxic effects of PL in Cal27 cells.

Conclusions

These findings suggest that PL exhibits potent inhibitory effects on TRPM7 and exerts its anti-cancer effects by downregulating TRPM7 expression and antagonizing channel currents.

研究目的阐明哌隆罗明（PL）的抗癌活性与其潜在靶点--口腔鳞状细胞癌（OSCC）中的瞬时受体电位美司他丁7通道（TRPM7）之间的关联：方法：在口腔鳞状细胞癌细胞系 Cal27 中研究了 TRPM7 的表达水平和电特性以及细胞活力对各种 PL 处理的反应：结果：PL处理导致Cal27细胞中TRPM7 mRNA和蛋白表达的浓度和时间依赖性降低。此外，PL 处理可抑制 Cal27 细胞中的 TRPM7 样整流电流；然而，这种抑制作用不如 TRPM7 拮抗剂 waixenicin A 的抑制作用有效。PL 的拮抗作用发生在 1 分钟内，并且在冲洗后没有完全逆转。值得注意的是，细胞外 Ca2+ 浓度仍然影响 PL 诱导的 TRPM7 样电流的变化，这表明 PL 可以直接但温和地拮抗 TRPM7 通道。TRPM7的功能变化与观察到的PL在Cal27细胞中的抗增殖和细胞毒性效应相关：这些研究结果表明，PL 对 TRPM7 具有强效抑制作用，并通过下调 TRPM7 表达和拮抗通道电流发挥其抗癌作用。

{"title":"Anti-cancer agent piperlongumine is an inhibitor of transient receptor potential melastatin 7 channel in oral squamous cell carcinoma","authors":"Tao Su , Yi-hui Chen , Kan-kui Wu , Xiao-hong Xu","doi":"10.1016/j.job.2024.03.002","DOIUrl":"10.1016/j.job.2024.03.002","url":null,"abstract":"<div><h3>Objectives</h3><p>To elucidate the association between the anticancer activities of piperlongumine (PL) and its potential target, transient receptor potential melastatin 7 channel (TRPM7), in oral squamous cell carcinoma (OSCC).</p></div><div><h3>Methods</h3><p>The expression levels and electrical characteristics of TRPM7 as well as cell viability in response to various PL treatments were investigated in the OSCC cell line Cal27.</p></div><div><h3>Results</h3><p>PL treatment resulted in a concentration- and time-dependent reduction in <em>TRPM7</em> mRNA and protein expression in Cal27 cells. Furthermore, PL treatment inhibited TRPM7-like rectifying currents in Cal27 cells; however, this inhibition was less effective than that of the TRPM7 antagonist waixenicin A. Rapid perfusion and washout experiments revealed an immediate inhibitory effect of PL on TRPM7-like currents. The antagonistic effect of PL occurred within 1 min and was not completely reversed following washout. Notably, the extracellular Ca<sup>2+</sup> concentration still influenced PL-induced changes in the TRPM7-like current, indicating that PL can directly but gently antagonize the TRPM7 channel. Functional changes in TRPM7 correlated with the observed antiproliferative and cytotoxic effects of PL in Cal27 cells.</p></div><div><h3>Conclusions</h3><p>These findings suggest that PL exhibits potent inhibitory effects on TRPM7 and exerts its anti-cancer effects by downregulating TRPM7 expression and antagonizing channel currents.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 430-438"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140060723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional roles of descending projections from the cerebral cortex to the trigeminal spinal subnucleus caudalis in orofacial nociceptive information processing 大脑皮层向三叉神经脊髓尾状核的降序投射在口面部痛觉信息处理中的功能作用

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.05.004

Masayuki Kobayashi , Yuka Nakaya , Satomi Kobayashi

Background

The trigeminal spinal subnucleus caudalis (Sp5C), also known as the medullary dorsal horn, receives orofacial somatosensory inputs, particularly nociceptive inputs, from the trigeminal nerve. In the Sp5C, excitatory and inhibitory neurons, glutamatergic and GABAergic/glycinergic neurons, respectively, form the local circuits. The axons of the glutamatergic neurons in lamina I ascend toward the thalamic and parabrachial nuclei, and this projection is the main pathway of orofacial nociception. Additionally, the axons of the higher brain regions, including the locus coeruleus, dorsal raphe, and cerebral cortex, are sent to the Sp5C.

Highlight

Among these descending projections, this review focuses on the functional profiles of the corticotrigeminal projections to the Sp5C, along with their anatomical aspects. The primary and secondary somatosensory and insular cortices are of particular interest.

Conclusion

Corticotrigeminal projections from the somatosensory cortex to the Sp5C play a suppressive role in nociceptive information processing, whereas recent studies have demonstrated a facilitative role of the insular cortex in nociceptive information processing at the Sp5C level.

背景：三叉神经脊髓尾下核（Sp5C）又称延髓背角，接收来自三叉神经的口面部躯体感觉输入，尤其是痛觉输入。在 Sp5C 中，兴奋性神经元和抑制性神经元，即谷氨酸能神经元和 GABA 能/甘氨肽能神经元，分别构成了局部回路。Ⅰ层谷氨酸能神经元的轴突向丘脑和颊旁核上升，这一投射是口面部痛觉的主要途径。此外，高级脑区的轴突，包括脑室、背侧剑突和大脑皮层，也被发送到 Sp5C.Highlight：在这些降序投射中，本综述将重点讨论皮质三叉神经投射到 Sp5C 的功能特征及其解剖学方面。初级和次级躯体感觉皮层和岛叶皮层尤其值得关注：结论：从躯体感觉皮层到 Sp5C 的皮质三叉神经投射在痛觉信息处理中起抑制作用，而最近的研究表明，岛叶皮层在 Sp5C 水平的痛觉信息处理中起促进作用。

{"title":"Functional roles of descending projections from the cerebral cortex to the trigeminal spinal subnucleus caudalis in orofacial nociceptive information processing","authors":"Masayuki Kobayashi , Yuka Nakaya , Satomi Kobayashi","doi":"10.1016/j.job.2024.05.004","DOIUrl":"10.1016/j.job.2024.05.004","url":null,"abstract":"<div><h3>Background</h3><p>The trigeminal spinal subnucleus caudalis (Sp5C), also known as the medullary dorsal horn, receives orofacial somatosensory inputs, particularly nociceptive inputs, from the trigeminal nerve. In the Sp5C, excitatory and inhibitory neurons, glutamatergic and GABAergic/glycinergic neurons, respectively, form the local circuits. The axons of the glutamatergic neurons in lamina I ascend toward the thalamic and parabrachial nuclei, and this projection is the main pathway of orofacial nociception. Additionally, the axons of the higher brain regions, including the locus coeruleus, dorsal raphe, and cerebral cortex, are sent to the Sp5C.</p></div><div><h3>Highlight</h3><p>Among these descending projections, this review focuses on the functional profiles of the corticotrigeminal projections to the Sp5C, along with their anatomical aspects. The primary and secondary somatosensory and insular cortices are of particular interest.</p></div><div><h3>Conclusion</h3><p>Corticotrigeminal projections from the somatosensory cortex to the Sp5C play a suppressive role in nociceptive information processing, whereas recent studies have demonstrated a facilitative role of the insular cortex in nociceptive information processing at the Sp5C level.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 304-307"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140909455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor microenvironment of ameloblastoma with a focus on osteoclastogenesis, cell migration, and malignant transformation 骨髓母细胞瘤的肿瘤微环境，重点关注破骨细胞生成、细胞迁移和恶性转化。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.05.002

Shohei Yoshimoto , Kazuhiko Okamura

Background

Odontogenic tumors arise in the jawbone and originate from cells associated with tooth development. Therefore, understanding odontogenic tumors requires knowledge of all aspects of dental research, including tooth development and eruption. Ameloblastoma is the most common odontogenic tumor.

Highlight

Although a benign tumor, ameloblastoma progresses with marked jawbone resorption. Because of its locally aggressive features, it can be treated surgically by resecting the surrounding bone. From a molecular pathology perspective, several genetic mutations and dysregulated signaling pathways involved in ameloblastoma tumorigenesis have been identified. Histopathologically, ameloblastomas consist of peripheral ameloblast-like cells and an inner stellate reticulum. The stromal region consists of fibrovascular connective tissue, showing a characteristic sparse myxoid histology. In general, the tumor microenvironment, including the surrounding non-tumor cells, contributes to tumorigenesis and progression. In this review, we focus on the tumor microenvironment of ameloblastomas. In addition, we present some of our recent studies on osteoclastogenesis, tubulin acetylation-induced cell migration, and hypoxia-induced epithelial–mesenchymal transition in ameloblastomas.

Conclusion

Further research on ameloblastomas can lead to the development of new treatments and improve patients’ quality of life.

背景：牙源性肿瘤发生于颌骨，源于与牙齿发育相关的细胞。因此，了解牙源性肿瘤需要了解牙科研究的各个方面，包括牙齿发育和萌出。釉母细胞瘤是最常见的牙源性肿瘤：亮点：釉母细胞瘤虽然是一种良性肿瘤，但其发展过程中会出现明显的颌骨吸收。由于其局部侵袭性特征，可通过手术切除周围骨质进行治疗。从分子病理学的角度来看，已经发现有几种基因突变和信号通路失调参与了绒母细胞瘤的肿瘤发生。从组织病理学角度看，绒母细胞瘤由外周绒母细胞样细胞和内部星状网状细胞组成。基质区由纤维血管结缔组织组成，呈现出特有的稀疏肌样组织学。一般来说，肿瘤微环境（包括周围的非肿瘤细胞）有助于肿瘤的发生和发展。在这篇综述中，我们将重点讨论母细胞瘤的肿瘤微环境。此外，我们还介绍了近期关于破骨细胞生成、微管蛋白乙酰化诱导的细胞迁移以及缺氧诱导的上皮-间质转化的一些研究：结论：对牙釉质母细胞瘤的进一步研究可开发出新的治疗方法，改善患者的生活质量。

{"title":"Tumor microenvironment of ameloblastoma with a focus on osteoclastogenesis, cell migration, and malignant transformation","authors":"Shohei Yoshimoto , Kazuhiko Okamura","doi":"10.1016/j.job.2024.05.002","DOIUrl":"10.1016/j.job.2024.05.002","url":null,"abstract":"<div><h3>Background</h3><p>Odontogenic tumors arise in the jawbone and originate from cells associated with tooth development. Therefore, understanding odontogenic tumors requires knowledge of all aspects of dental research, including tooth development and eruption. Ameloblastoma is the most common odontogenic tumor.</p></div><div><h3>Highlight</h3><p>Although a benign tumor, ameloblastoma progresses with marked jawbone resorption. Because of its locally aggressive features, it can be treated surgically by resecting the surrounding bone. From a molecular pathology perspective, several genetic mutations and dysregulated signaling pathways involved in ameloblastoma tumorigenesis have been identified. Histopathologically, ameloblastomas consist of peripheral ameloblast-like cells and an inner stellate reticulum. The stromal region consists of fibrovascular connective tissue, showing a characteristic sparse myxoid histology. In general, the tumor microenvironment, including the surrounding non-tumor cells, contributes to tumorigenesis and progression. In this review, we focus on the tumor microenvironment of ameloblastomas. In addition, we present some of our recent studies on osteoclastogenesis, tubulin acetylation-induced cell migration, and hypoxia-induced epithelial–mesenchymal transition in ameloblastomas.</p></div><div><h3>Conclusion</h3><p>Further research on ameloblastomas can lead to the development of new treatments and improve patients’ quality of life.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 314-319"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140909462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of tooth loss on behavioral and psychological symptoms of dementia in app knock-in mice 缺牙对 app 基因敲入小鼠痴呆症的行为和心理症状的影响。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.03.005

Masae Furukawa , Hirobumi Tada , Resmi Raju , Jingshu Wang , Haruna Yokoi , Mitsuyoshi Yamada , Yosuke Shikama , Takashi Saito , Takaomi C. Saido , Kenji Matsushita

Objectives

Many patients with Alzheimer's disease (AD) experience behavioral and psychological symptoms of dementia (BPSD), which significantly affect their quality of life. It is known that 5-Hydroxytryptamine (5-HT) plays a crucial role in the development of BPSD. While the relationship between tooth loss and AD symptoms has been acknowledged, the aspect of aggression has not been focused on until now. Despite the established importance of 5-HT in BPSD, how tooth loss is related to the exacerbation of AD symptoms, especially in terms of aggression, remains largely unexplored. Although nutritional status is known to influence the progression of dementia, the specific effect of tooth loss on peripheral symptoms, notably aggression, is not well understood.

Methods

In our study, we conducted maxillary molar extractions in aged C57BL/6J and App^NL-G-F mice and observed their condition over a 3-month period. During this time, we documented significant behavioral and genetic differences between mice in the control groups and mice that underwent tooth extraction. Notably, mice that underwent tooth extraction exhibited a considerable decline in cognitive function and increased in aggression 3 months after tooth extraction compared with the control groups (C57BL/6J and App^NL-G-Fmice).

Results

Our findings suggest that molar loss may lead to reduced 5-HT levels in the hippocampus, possibly mediated by the trigeminal nerve, contributing to the development of aggression and BPSD in AD.

Conclusion

This study sheds light on the intricate relationships between oral health, 5-HT, and AD symptoms, offering valuable insights into potential therapeutic avenues for managing BPSD in patients with dementia.

目的：许多阿尔茨海默病患者都会出现痴呆的行为和心理症状（BPSD），这严重影响了他们的生活质量。众所周知，5-羟色胺（5-HT）在 BPSD 的发展过程中起着至关重要的作用。然而，牙齿脱落与阿尔茨海默病症状（尤其是攻击行为）之间的关系仍未得到研究。虽然众所周知营养状况会影响痴呆症的进展，但牙齿缺失对外周症状（尤其是攻击性）的具体影响却不甚了解：在我们的研究中，我们对年老的 C57BL6J 和 AppNL-G-F 小鼠进行了上颌臼齿拔除，并观察了它们 3 个月的状况。在此期间，我们记录了对照组小鼠与拔牙组小鼠在行为和遗传方面的显著差异。值得注意的是，与对照组（C57BL6J 或 AppNL-G-F 小鼠）相比，拔牙后 3 个月的小鼠认知功能显著下降，攻击性增强：我们的研究结果表明，臼齿脱落可能会导致海马中的5-羟色胺水平降低，这可能是由三叉神经介导的，从而导致阿尔茨海默病中攻击性和BPSD的发生：本研究揭示了口腔健康、5-羟色胺和阿尔茨海默病症状之间错综复杂的关系，为治疗痴呆症患者的BPSD提供了有价值的见解。

{"title":"Effects of tooth loss on behavioral and psychological symptoms of dementia in app knock-in mice","authors":"Masae Furukawa , Hirobumi Tada , Resmi Raju , Jingshu Wang , Haruna Yokoi , Mitsuyoshi Yamada , Yosuke Shikama , Takashi Saito , Takaomi C. Saido , Kenji Matsushita","doi":"10.1016/j.job.2024.03.005","DOIUrl":"10.1016/j.job.2024.03.005","url":null,"abstract":"<div><h3>Objectives</h3><p>Many patients with Alzheimer's disease (AD) experience behavioral and psychological symptoms of dementia (BPSD), which significantly affect their quality of life. It is known that 5-Hydroxytryptamine (5-HT) plays a crucial role in the development of BPSD. While the relationship between tooth loss and AD symptoms has been acknowledged, the aspect of aggression has not been focused on until now. Despite the established importance of 5-HT in BPSD, how tooth loss is related to the exacerbation of AD symptoms, especially in terms of aggression, remains largely unexplored. Although nutritional status is known to influence the progression of dementia, the specific effect of tooth loss on peripheral symptoms, notably aggression, is not well understood.</p></div><div><h3>Methods</h3><p>In our study, we conducted maxillary molar extractions in aged C57BL/6J and <em>App</em><sup><em>NL-G-F</em></sup> mice and observed their condition over a 3-month period. During this time, we documented significant behavioral and genetic differences between mice in the control groups and mice that underwent tooth extraction. Notably, mice that underwent tooth extraction exhibited a considerable decline in cognitive function and increased in aggression 3 months after tooth extraction compared with the control groups (C57BL/6J and <em>App</em><sup><em>NL-G-F</em></sup>mice).</p></div><div><h3>Results</h3><p>Our findings suggest that molar loss may lead to reduced 5-HT levels in the hippocampus, possibly mediated by the trigeminal nerve, contributing to the development of aggression and BPSD in AD.</p></div><div><h3>Conclusion</h3><p>This study sheds light on the intricate relationships between oral health, 5-HT, and AD symptoms, offering valuable insights into potential therapeutic avenues for managing BPSD in patients with dementia.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 329-338"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1349007924000744/pdfft?md5=5ddb384ef8a89c918dfd65de96394830&pid=1-s2.0-S1349007924000744-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140194811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transposon insertion in Rothia dentocariosa Rothia dentocariosa 中的转座子插入。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.04.006

Boang Liu , Chiho Mashimo , Takayuki Nambu , Hugo Maruyama , Toshinori Okinaga

Objectives

Rothia spp. are emerging as significant bacteria associated with oral health, with Rothia dentocariosa being one of the most prevalent species. However, there is a lack of studies examining these properties at the genetic level. This study aimed to establish a genetic modification platform for R. dentocariosa.

Methods

Rothia spp. were isolated from saliva samples collected from healthy volunteers. Subsequently, R. dentocariosa strains were identified through colony morphology, species-specific polymerase chain reaction (PCR), and 16S ribosomal RNA gene sequencing. The identified strains were then transformed with plasmid pJRD215, and the most efficient strain was selected. Transposon insertion mutagenesis was performed to investigate the possibility of genetic modifications.

Results

A strain demonstrating high transforming ability, designated as R. dentocariosa LX16, was identified. This strain underwent transposon insertion mutagenesis and was screened for 5-fluoroorotic acid-resistant transposants. The insertion sites were confirmed using arbitrary primed PCR, gene-specific PCR, and Sanger sequencing.

Conclusion

This study marks the first successful genetic modification of R. dentocariosa. Investigating R. dentocariosa at the genetic level can provide insights into its role within the oral microbiome.

目的齿槽菌属正在成为与口腔健康相关的重要细菌，其中齿槽菌是最普遍的种类之一。然而，目前还缺乏从基因层面研究这些特性的研究。本研究的目的是为牙关紧闭杆菌建立一个基因改造平台。方法从健康志愿者的唾液样本中分离出牙关紧闭杆菌。随后，通过菌落形态学、物种特异性聚合酶链式反应（PCR）和 16S 核糖体 RNA 基因测序鉴定出牙关紧闭杆菌菌株。然后用质粒 pJRD215 对鉴定出的菌株进行转化，选出效率最高的菌株。结果发现了一株转化能力很强的菌株，命名为 R. dentocariosa LX16。对该菌株进行了转座子插入诱变，并筛选出了抗 5-氟乳清酸的转座子。使用任意引物 PCR、基因特异性 PCR 和 Sanger 测序对插入位点进行了确认。在基因水平上研究 R. dentocariosa 可以深入了解其在口腔微生物组中的作用。

{"title":"Transposon insertion in Rothia dentocariosa","authors":"Boang Liu , Chiho Mashimo , Takayuki Nambu , Hugo Maruyama , Toshinori Okinaga","doi":"10.1016/j.job.2024.04.006","DOIUrl":"10.1016/j.job.2024.04.006","url":null,"abstract":"<div><h3>Objectives</h3><p><em>Rothia</em> spp. are emerging as significant bacteria associated with oral health, with <em>Rothia dentocariosa</em> being one of the most prevalent species. However, there is a lack of studies examining these properties at the genetic level. This study aimed to establish a genetic modification platform for <em>R. dentocariosa.</em></p></div><div><h3>Methods</h3><p><em>Rothia</em> spp. were isolated from saliva samples collected from healthy volunteers. Subsequently, <em>R. dentocariosa</em> strains were identified through colony morphology, species-specific polymerase chain reaction (PCR), and 16S ribosomal RNA gene sequencing. The identified strains were then transformed with plasmid pJRD215, and the most efficient strain was selected. Transposon insertion mutagenesis was performed to investigate the possibility of genetic modifications.</p></div><div><h3>Results</h3><p>A strain demonstrating high transforming ability, designated as <em>R. dentocariosa</em> LX16, was identified. This strain underwent transposon insertion mutagenesis and was screened for 5-fluoroorotic acid-resistant transposants. The insertion sites were confirmed using arbitrary primed PCR, gene-specific PCR, and Sanger sequencing.</p></div><div><h3>Conclusion</h3><p>This study marks the first successful genetic modification of <em>R. dentocariosa</em>. Investigating <em>R. dentocariosa</em> at the genetic level can provide insights into its role within the oral microbiome.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 358-364"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140785624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Title Page (without cities) 扉页（不含城市）

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/S1349-0079(24)00096-3

引用次数: 0

Osteoblast differentiation of Gli1⁺ cells via Wnt and BMP signaling pathways during orthodontic tooth movement 在牙齿矫正过程中，Gli1⁺细胞通过 Wnt 和 BMP 信号通路进行成骨细胞分化。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.03.004

Yuri Seki , Hiroaki Takebe , Yuya Nakao , Kohei Sato , Toshihide Mizoguchi , Hiroaki Nakamura , Masahiro Iijima , Akihiro Hosoya

Objectives

Factors that induce bone formation during orthodontic tooth movement (OTM) remain unclear. Gli1 was recently identified as a stem cell marker in the periodontal ligament (PDL). Therefore, we evaluated the mechanism of differentiation of Cre/LoxP-mediated Gli1/Tomato⁺ cells into osteoblasts during OTM.

Methods

After the final administration of tamoxifen to 8-week-old Gli1-Cre^ERT2/ROSA26-loxP-stop-loxP-tdTomato mice for 2 days, nickel–titanium closed coil springs were attached between the upper anterior alveolar bone and the first molar. Immunohistochemical localizations of β-catenin, Smad4, and Runx2 were observed in the PDL on 2, 5, and 10 days after OTM initiation.

Results

In the untreated tooth, few Gli1/Tomato⁺ cells were detected in the PDL. Two days after OTM initiation, the number of Gli1/Tomato⁺ cells increased in the PDL on the tension side. On this side, 49.3 ± 7.0% of β-catenin⁺ and 48.7 ± 5.7% of Smad4⁺ cells were found in the PDL, and Runx2 expression was detected in some Gli1/Tomato⁺ cells apart from the alveolar bone. The number of positive cells in the PDL reached a maximum on day 5. In contrast, on the compression side, β-catenin and Smad4 exhibited less immunoreactivity. On day 10, Gli1/Tomato⁺ cells were aligned on the alveolar bone on the tension side, with some expressing Runx2.

Conclusions

Gli1⁺ cells in the PDL differentiated into osteoblasts during OTM. Wnt and bone morphogenetic proteins signaling pathways may be involved in this differentiation.

目的：正畸牙齿移动（OTM）过程中诱导骨形成的因素仍不清楚。最近发现 Gli1 是牙周韧带 (PDL) 中的干细胞标记。因此，我们评估了OTM期间Cre/LoxP介导的Gli1/Tomato+细胞向成骨细胞分化的机制：方法：给 8 周龄的 Gli1-CreERT2/ROSA26-loxP-stop-loxP-tdTomato 小鼠注射他莫昔芬 2 天后，在上前牙槽骨和第一磨牙之间连接镍钛闭合螺旋弹簧。结果显示：在OTM开始后的2天、5天和10天，在PDL中观察了β-catenin、Smad4和Runx2的免疫组化定位：结果：在未处理的牙齿中，PDL 中检测到少量 Gli1/Tomato+ 细胞。开始 OTM 两天后，张力侧 PDL 中 Gli1/Tomato+ 细胞数量增加。在这一侧，PDL中发现了49.3±7.0%的β-catenin+细胞和48.7±5.7%的Smad4+细胞，在除牙槽骨外的一些Gli1/Tomato+细胞中检测到了Runx2的表达。PDL 中阳性细胞的数量在第 5 天达到最大值。相比之下，在压迫侧，β-catenin 和 Smad4 的免疫活性较低。第10天，Gli1/Tomato+细胞排列在张力侧的牙槽骨上，部分细胞表达Runx2：结论：PDL中的Gli1+细胞在OTM过程中分化为成骨细胞。结论：PDL中的Gli1+细胞在OTM过程中分化为成骨细胞，Wnt和骨形态发生蛋白信号通路可能参与了这种分化。

{"title":"Osteoblast differentiation of Gli1⁺ cells via Wnt and BMP signaling pathways during orthodontic tooth movement","authors":"Yuri Seki , Hiroaki Takebe , Yuya Nakao , Kohei Sato , Toshihide Mizoguchi , Hiroaki Nakamura , Masahiro Iijima , Akihiro Hosoya","doi":"10.1016/j.job.2024.03.004","DOIUrl":"10.1016/j.job.2024.03.004","url":null,"abstract":"<div><h3>Objectives</h3><p>Factors that induce bone formation during orthodontic tooth movement (OTM) remain unclear. Gli1 was recently identified as a stem cell marker in the periodontal ligament (PDL). Therefore, we evaluated the mechanism of differentiation of Cre/LoxP-mediated Gli1/Tomato<sup>+</sup> cells into osteoblasts during OTM.</p></div><div><h3>Methods</h3><p>After the final administration of tamoxifen to 8-week-old Gli1-Cre<sup>ERT2</sup>/ROSA26-loxP-stop-loxP-tdTomato mice for 2 days, nickel–titanium closed coil springs were attached between the upper anterior alveolar bone and the first molar. Immunohistochemical localizations of β-catenin, Smad4, and Runx2 were observed in the PDL on 2, 5, and 10 days after OTM initiation.</p></div><div><h3>Results</h3><p>In the untreated tooth, few Gli1/Tomato<sup>+</sup> cells were detected in the PDL. Two days after OTM initiation, the number of Gli1/Tomato<sup>+</sup> cells increased in the PDL on the tension side. On this side, 49.3 ± 7.0% of β-catenin<sup>+</sup> and 48.7 ± 5.7% of Smad4<sup>+</sup> cells were found in the PDL, and Runx2 expression was detected in some Gli1/Tomato<sup>+</sup> cells apart from the alveolar bone. The number of positive cells in the PDL reached a maximum on day 5. In contrast, on the compression side, β-catenin and Smad4 exhibited less immunoreactivity. On day 10, Gli1/Tomato<sup>+</sup> cells were aligned on the alveolar bone on the tension side, with some expressing Runx2.</p></div><div><h3>Conclusions</h3><p>Gli1<sup>+</sup> cells in the PDL differentiated into osteoblasts during OTM. Wnt and bone morphogenetic proteins signaling pathways may be involved in this differentiation.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 373-380"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140159190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of citrullinated α1-antitrypsin (A1AT) in saliva in a mouse model of rheumatoid arthritis 鉴定类风湿性关节炎小鼠模型唾液中的瓜氨酸化α1-抗胰蛋白酶（A1AT）。

IF 2.4 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2024-06-01 DOI: 10.1016/j.job.2024.03.007

Wakako Sakaguchi , Juri Saruta , Yuko Yamamoto , Tomoko Shimizu , Shinya Fuchida , Keiichi Tsukinoki

Objectives

Rheumatoid arthritis (RA) is an autoimmune disease characterized by progressive joint destruction. Early diagnosis and treatment, before joint deformation or destruction occurs, are crucial. Identifying novel biomarkers for RA in saliva could potentially enable early detection of the disease, prior to its onset.

Methods

We conducted a comprehensive proteomic analysis of salivary proteins in a mouse model of RA. Proteins were identified using western blotting and enzyme-linked immunosorbent assay in the serum, saliva, and ankle joints of DBA/1JJmsSlc mice, a model of RA. Ankle joints and submandibular glands were stained with hematoxylin and eosin and immunostained, and the results were compared with those of control mice.

Results

Citrullinated alpha-1 antitrypsin (A1AT, 46 kDa) was commonly detected in the saliva, serum, and ankle joints of mice with severe RA and was confirmed through proteomic analysis. Western blotting showed a band corresponding to 46 kDa in the serum, saliva, and ankle joints. Immunostaining of the ankle joints with the A1AT antibody showed a strong positive signal in the synovium.

Conclusions

In DBA/1JJmsSlc mice, cyclic citrullinated peptide antibodies and A1AT may be involved in citrullination and contribute to the development and severity of RA, making them valuable treatment targets requiring further study.

目的：类风湿性关节炎（RA）是一种以进行性关节破坏为特征的自身免疫性疾病。在关节变形或破坏发生之前进行早期诊断和治疗至关重要。鉴定唾液中的新型类风湿性关节炎生物标志物有可能在疾病发作前对其进行早期检测：我们对RA小鼠模型的唾液蛋白质进行了全面的蛋白质组学分析。我们使用 Western 印迹法和酶联免疫吸附法鉴定了 DBA/1JJmsSlc 小鼠（一种 RA 模型）血清、唾液和踝关节中的蛋白质。对踝关节和颌下腺进行苏木精和伊红染色及免疫染色，并将结果与对照组小鼠进行比较：结果：在严重RA小鼠的唾液、血清和踝关节中普遍检测到瓜氨酸化α-1抗胰蛋白酶（A1AT，46 kDa），并通过蛋白质组分析得到证实。Western 印迹显示，在血清、唾液和踝关节中都有一条与 46 kDa 相对应的条带。用 A1AT 抗体对踝关节进行免疫染色显示，滑膜中出现了强烈的阳性信号：结论：在DBA/1JJmsSlc小鼠中，环瓜氨酸肽抗体和A1AT可能参与瓜氨酸化，并导致RA的发生和严重程度，因此是有价值的治疗靶点，需要进一步研究。

{"title":"Identification of citrullinated α1-antitrypsin (A1AT) in saliva in a mouse model of rheumatoid arthritis","authors":"Wakako Sakaguchi , Juri Saruta , Yuko Yamamoto , Tomoko Shimizu , Shinya Fuchida , Keiichi Tsukinoki","doi":"10.1016/j.job.2024.03.007","DOIUrl":"10.1016/j.job.2024.03.007","url":null,"abstract":"<div><h3>Objectives</h3><p>Rheumatoid arthritis (RA) is an autoimmune disease characterized by progressive joint destruction. Early diagnosis and treatment, before joint deformation or destruction occurs, are crucial. Identifying novel biomarkers for RA in saliva could potentially enable early detection of the disease, prior to its onset.</p></div><div><h3>Methods</h3><p>We conducted a comprehensive proteomic analysis of salivary proteins in a mouse model of RA. Proteins were identified using western blotting and enzyme-linked immunosorbent assay in the serum, saliva, and ankle joints of DBA/1JJmsSlc mice, a model of RA. Ankle joints and submandibular glands were stained with hematoxylin and eosin and immunostained, and the results were compared with those of control mice.</p></div><div><h3>Results</h3><p>Citrullinated alpha-1 antitrypsin (A1AT, 46 kDa) was commonly detected in the saliva, serum, and ankle joints of mice with severe RA and was confirmed through proteomic analysis. Western blotting showed a band corresponding to 46 kDa in the serum, saliva, and ankle joints. Immunostaining of the ankle joints with the A1AT antibody showed a strong positive signal in the synovium.</p></div><div><h3>Conclusions</h3><p>In DBA/1JJmsSlc mice, cyclic citrullinated peptide antibodies and A1AT may be involved in citrullination and contribute to the development and severity of RA, making them valuable treatment targets requiring further study.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 2","pages":"Pages 473-482"},"PeriodicalIF":2.4,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140330215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0