Journal of Oral Biosciences最新文献_第6页

Determination of Streptococcus gordonii novel adhesins specific to sialidase-treated erythrocytes 唾液酸酶处理红细胞特异性戈多链球菌新型黏附素的测定

IF 2.3 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-10-12 DOI: 10.1016/j.job.2025.100700

Mayumi Uchikawa, Keitarou Saiki, Yuiko Ishikawa, Yumiko Urano-Tashiro, Yuki Yamanaka, Yukihiro Takahashi

Objectives

The Hsa/GspB adhesins of the oral bacterium Streptococcus gordonii are glycoproteins that bind to sialic acid. Hsa/GspB are peptidoglycan-associated proteins that have the Leu-Pro-X-Thr-Gly (LPXTG) motif and they have sialic acid-dependent hemagglutinating (HA) activity. In S. gordonii DL1, Hsa is the only HA factor for human erythrocytes, whereas the clinical isolate, S. gordonii NDU1118, has sialic-acid-dependent HA activity via GspB and distinct sialic-acid-independent HA activity. This study aimed to identify sialic-acid-independent adhesins in S. gordonii NDU1118.

Methods

The complete genome of the NDU1118 strain was sequenced and annotated. LPXTG-containing proteins were identified and compared with those of the DL1 strain to select candidate genes unique to the NDU1118 strain. The candidate genes were individually deleted to construct mutant strains, which were then tested for sialic-acid-independent HA activity in neuraminidase (sialidase)-treated human erythrocytes.

Results

Genome sequencing of S. gordonii NDU1118 identified 31 putative peptidoglycan-associated proteins containing the LPXTG motif. Homology analysis revealed that NDA_1151 and NDA_2061 did not have homologs in the DL1 strain and were therefore selected as potential adhesin candidates. Reverse transcription-PCR confirmed that both genes were transcribed in the NDU1118 strain. Deletion of NDA_1151 or NDA_2061 abolished HA activity in neuraminidase-treated erythrocytes.

Conclusions

NDA_1151 and NDA_2061 encode novel sialic-acid-independent adhesins, designated aggregation of sialidase-treated hematids 1 (ash1) and ash2, respectively.

目的口腔细菌戈登链球菌的Hsa/GspB黏附素是一种与唾液酸结合的糖蛋白。Hsa/GspB是具有Leu-Pro-X-Thr-Gly （LPXTG）基序的肽聚糖相关蛋白，它们具有唾液酸依赖性血凝（HA）活性。在gordonii DL1中，Hsa是人类红细胞中唯一的HA因子，而临床分离的S. gordonii NDU1118通过GspB具有唾液酸依赖性HA活性和明显的唾液酸非依赖性HA活性。本研究旨在鉴定鼠链球菌NDU1118中不依赖唾液酸的粘附素。方法对NDU1118菌株进行全基因组测序和注释。鉴定含有lpxtg的蛋白，并与DL1菌株的蛋白进行比较，选择NDU1118菌株特有的候选基因。候选基因被单独删除以构建突变株，然后在神经氨酸酶（唾液酸酶）处理的人红细胞中检测唾液酸非依赖性HA活性。结果菌株NDU1118的基因组测序鉴定出31个含有LPXTG基序的肽聚糖相关蛋白。同源性分析显示，NDA_1151和NDA_2061在DL1菌株中没有同源物，因此被选为潜在的粘附素候选物。反转录pcr证实这两个基因在NDU1118菌株中均有转录。NDA_1151或NDA_2061的缺失使神经氨酸酶处理的红细胞HA活性降低。结论snda_1151和NDA_2061编码新的唾液酸非依赖性粘附素，分别指定唾液酸酶处理的血肿1 （ash1）和ash2的聚集。

{"title":"Determination of Streptococcus gordonii novel adhesins specific to sialidase-treated erythrocytes","authors":"Mayumi Uchikawa, Keitarou Saiki, Yuiko Ishikawa, Yumiko Urano-Tashiro, Yuki Yamanaka, Yukihiro Takahashi","doi":"10.1016/j.job.2025.100700","DOIUrl":"10.1016/j.job.2025.100700","url":null,"abstract":"<div><h3>Objectives</h3><div>The Hsa/GspB adhesins of the oral bacterium <em>Streptococcus gordonii</em> are glycoproteins that bind to sialic acid. Hsa/GspB are peptidoglycan-associated proteins that have the Leu-Pro-X-Thr-Gly (LPXTG) motif and they have sialic acid-dependent hemagglutinating (HA) activity. In <em>S. gordonii</em> DL1, Hsa is the only HA factor for human erythrocytes, whereas the clinical isolate, <em>S. gordonii</em> NDU1118, has sialic-acid-dependent HA activity via GspB and distinct sialic-acid-independent HA activity. This study aimed to identify sialic-acid-independent adhesins in <em>S. gordonii</em> NDU1118.</div></div><div><h3>Methods</h3><div>The complete genome of the NDU1118 strain was sequenced and annotated. LPXTG-containing proteins were identified and compared with those of the DL1 strain to select candidate genes unique to the NDU1118 strain. The candidate genes were individually deleted to construct mutant strains, which were then tested for sialic-acid-independent HA activity in neuraminidase (sialidase)-treated human erythrocytes.</div></div><div><h3>Results</h3><div>Genome sequencing of <em>S. gordonii</em> NDU1118 identified 31 putative peptidoglycan-associated proteins containing the LPXTG motif. Homology analysis revealed that NDA_1151 and NDA_2061 did not have homologs in the DL1 strain and were therefore selected as potential adhesin candidates. Reverse transcription-PCR confirmed that both genes were transcribed in the NDU1118 strain. Deletion of <em>NDA_1151</em> or <em>NDA_2061</em> abolished HA activity in neuraminidase-treated erythrocytes.</div></div><div><h3>Conclusions</h3><div><em>NDA_1151</em> and <em>NDA_2061</em> encode novel sialic-acid-independent adhesins, designated aggregation of sialidase-treated hematids 1 (<em>ash1</em>) and <em>ash2</em>, respectively.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100700"},"PeriodicalIF":2.3,"publicationDate":"2025-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145332475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preference for taste solutions within 10 minutes after starting to drink in water-deprived rats 缺水大鼠开始饮用后10分钟内对味道溶液的偏好

IF 2.3 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-10-11 DOI: 10.1016/j.job.2025.100701

Izumi Manabe , Maho Yamazaki , Shinpei Takahashi , Shusuke Iwata , Toshiaki Yasuo , Takeshi Suwabe , Satoshi Kawano , Noritaka Sako

Objective

To evaluate the preference for taste solutions with palatable and aversive qualities during the initial 10 min after resuming drinking in water-deprived rats.

Methods

The effects of pre-exposure to taste solutions in eight-week-old male Wistar/ST rats and the impact of post-ingestive effects were examined by two-bottle preference tests every 2 min over a 10-min period for five days after deprived of water for 24 h. Sodium saccharin (Sacc, without post-ingestive effects) and sucrose (Suc, with post-ingestive effects) were used in the palatable solutions, and quinine hydrochloride (Q) was used in the aversive solution.

Results

In naïve rats, a significant difference in the consumed volume of Q solution and distilled water (DW) was observed on Day 1, whereas differences for the Sacc and Suc solutions were observed on Day 4. In pre-exposed rats, differences for the Sacc, Q, and Suc solutions were observed on Day 1, Day 1, and Day 2, respectively. However, no differences were observed for the Sacc solution on Days 2, 4, and 5. The cumulative volume consumed within 10 min differed significantly among all taste solutions and DW for almost all experimental days in the naïve and pre-exposed groups.

Conclusions

The short-term test showed that water-deprived rats did not initially discriminate between the qualities of taste solutions when commencing drinking after deprivation. Comparisons between the naïve and pre-exposed groups revealed that drinking behavior depended on prior exposure to the solutions. The short-term tests with the Sacc and Suc solutions indicate that post-ingestive effects influence drinking behavior.

目的探讨缺水大鼠在恢复饮水后最初10 min内对具有可口性和厌恶性的味觉溶液的偏好。方法8周龄雄性Wistar/ST大鼠在断水24 h后，连续5天，每隔10 min，每隔2 min进行两瓶偏好试验，观察味觉溶液的预暴露效果和摄食后效应的影响。可口溶液采用糖精钠（Sacc，无摄食后效应）和蔗糖（Suc，有摄食后效应），厌恶溶液采用盐酸奎宁(Q)。结果naïve大鼠第1天Q溶液和蒸馏水（DW）的消耗量有显著差异，第4天Sacc和Suc溶液的消耗量有显著差异。在预暴露大鼠中，分别在第1天、第1天和第2天观察Sacc、Q和Suc溶液的差异。然而，Sacc溶液在第2、4和5天没有观察到差异。在naïve组和预暴露组中，几乎所有试验日期内，所有味觉溶液和DW的10分钟内累积消耗量都存在显著差异。短期试验表明，缺水大鼠在缺水后开始饮用时，最初并没有区分味道溶液的质量。naïve组和预先暴露组之间的比较表明，饮酒行为取决于先前暴露于溶液的情况。用Sacc和Suc溶液进行的短期试验表明，摄入后的影响会影响饮酒行为。

{"title":"Preference for taste solutions within 10 minutes after starting to drink in water-deprived rats","authors":"Izumi Manabe , Maho Yamazaki , Shinpei Takahashi , Shusuke Iwata , Toshiaki Yasuo , Takeshi Suwabe , Satoshi Kawano , Noritaka Sako","doi":"10.1016/j.job.2025.100701","DOIUrl":"10.1016/j.job.2025.100701","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the preference for taste solutions with palatable and aversive qualities during the initial 10 min after resuming drinking in water-deprived rats.</div></div><div><h3>Methods</h3><div>The effects of pre-exposure to taste solutions in eight-week-old male Wistar/ST rats and the impact of post-ingestive effects were examined by two-bottle preference tests every 2 min over a 10-min period for five days after deprived of water for 24 h. Sodium saccharin (Sacc, without post-ingestive effects) and sucrose (Suc, with post-ingestive effects) were used in the palatable solutions, and quinine hydrochloride (Q) was used in the aversive solution.</div></div><div><h3>Results</h3><div>In naïve rats, a significant difference in the consumed volume of Q solution and distilled water (DW) was observed on Day 1, whereas differences for the Sacc and Suc solutions were observed on Day 4. In pre-exposed rats, differences for the Sacc, Q, and Suc solutions were observed on Day 1, Day 1, and Day 2, respectively. However, no differences were observed for the Sacc solution on Days 2, 4, and 5. The cumulative volume consumed within 10 min differed significantly among all taste solutions and DW for almost all experimental days in the naïve and pre-exposed groups.</div></div><div><h3>Conclusions</h3><div>The short-term test showed that water-deprived rats did not initially discriminate between the qualities of taste solutions when commencing drinking after deprivation. Comparisons between the naïve and pre-exposed groups revealed that drinking behavior depended on prior exposure to the solutions. The short-term tests with the Sacc and Suc solutions indicate that post-ingestive effects influence drinking behavior.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100701"},"PeriodicalIF":2.3,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in 3D in vitro cell culture models for dental research 用于牙科研究的三维体外细胞培养模型的进展

IF 2.3 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-10-10 DOI: 10.1016/j.job.2025.100702

Selene G. Perales , Mia Rodriguez , Johnson Rajasingh , Ayman Al Dayeh , Yanhui Zhang , Douglas Dixon

Background

Recent advancements in three-dimensional (3D) in vitro models have transformed dental research, offering more accurate and physiologically relevant platforms to study oral health and diseases. This review explores the progress from basic spheroid models to sophisticated tooth-on-a-chip systems, highlighting their potential clinical applications. Spheroid models, which mimic the tissue architecture, offer valuable insights into cellular interactions and responses to various treatments. However, their simplicity limits their ability to replicate the complex oral cavity environments.

Highlight

Advancements made by transitioning from in vitro spheroid-based models to self-assembling 3D organoids or scaffold-based systems, which incorporate multiple cell types and extracellular matrix components, have enhanced the ability to mimic the native tissue environment and to study dental tissue development, regeneration, and pathological transition in greater detail. Similarly, the integration of microfluidic technology has led to the development of tooth-on-a-chip models that simulate the dynamic conditions of the oral cavity, including fluid flow and mechanical forces. These advanced models represent unprecedented opportunities for studying tooth development, disease progression, and the effects of dental treatments in a controlled and reproducible manner.

Conclusion

In vitro models, particularly 3D systems, offer promise for personalized medicine, allowing patient-specific testing of oral biomaterials and therapies. Concurrently, they also provide opportunities to study unique cell-to-cell interactions between host immune cells and putative bacteria, drugs, or antibiotic therapy/screening, potentially serving as an alternative to current animal models. This review highlights the importance of continued innovation in 3D in vitro modeling to advance understanding of dental biology and improve clinical outcomes.

三维体外模型的最新进展已经改变了牙科研究，为研究口腔健康和疾病提供了更准确和生理相关的平台。这篇综述探讨了从基本的球体模型到复杂的牙齿芯片系统的进展，强调了它们潜在的临床应用。球体模型，模仿组织结构，提供有价值的见解细胞相互作用和对各种治疗的反应。然而，它们的简单性限制了它们复制复杂口腔环境的能力。从体外球体为基础的模型过渡到自组装3D类器官或基于支架的系统所取得的进步，其中包含多种细胞类型和细胞外基质成分，增强了模拟天然组织环境的能力，并更详细地研究了牙齿组织的发育、再生和病理转变。同样，微流控技术的集成导致了模拟口腔动态条件的芯片上的牙齿模型的发展，包括流体流动和机械力。这些先进的模型为研究牙齿发育、疾病进展和牙科治疗的影响提供了前所未有的机会，这是一种可控和可重复的方式。结论体外模型，特别是3D系统，为个性化医疗提供了希望，允许对口腔生物材料和治疗进行患者特异性测试。同时，它们也为研究宿主免疫细胞与假定的细菌、药物或抗生素治疗/筛选之间独特的细胞间相互作用提供了机会，有可能作为当前动物模型的替代方案。这篇综述强调了3D体外建模持续创新的重要性，以促进对牙科生物学的理解和改善临床结果。

{"title":"Advancements in 3D in vitro cell culture models for dental research","authors":"Selene G. Perales , Mia Rodriguez , Johnson Rajasingh , Ayman Al Dayeh , Yanhui Zhang , Douglas Dixon","doi":"10.1016/j.job.2025.100702","DOIUrl":"10.1016/j.job.2025.100702","url":null,"abstract":"<div><h3>Background</h3><div>Recent advancements in three-dimensional (3D) in vitro models have transformed dental research, offering more accurate and physiologically relevant platforms to study oral health and diseases. This review explores the progress from basic spheroid models to sophisticated tooth-on-a-chip systems, highlighting their potential clinical applications. Spheroid models, which mimic the tissue architecture, offer valuable insights into cellular interactions and responses to various treatments. However, their simplicity limits their ability to replicate the complex oral cavity environments.</div></div><div><h3>Highlight</h3><div>Advancements made by transitioning from in vitro spheroid-based models to self-assembling 3D organoids or scaffold-based systems, which incorporate multiple cell types and extracellular matrix components, have enhanced the ability to mimic the native tissue environment and to study dental tissue development, regeneration, and pathological transition in greater detail. Similarly, the integration of microfluidic technology has led to the development of tooth-on-a-chip models that simulate the dynamic conditions of the oral cavity, including fluid flow and mechanical forces. These advanced models represent unprecedented opportunities for studying tooth development, disease progression, and the effects of dental treatments in a controlled and reproducible manner.</div></div><div><h3>Conclusion</h3><div>In vitro models, particularly 3D systems, offer promise for personalized medicine, allowing patient-specific testing of oral biomaterials and therapies. Concurrently, they also provide opportunities to study unique cell-to-cell interactions between host immune cells and putative bacteria, drugs, or antibiotic therapy/screening, potentially serving as an alternative to current animal models. This review highlights the importance of continued innovation in 3D in vitro modeling to advance understanding of dental biology and improve clinical outcomes.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100702"},"PeriodicalIF":2.3,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Altered expression of tenascin C in pericoronal tissues by odontogenic epithelial cells during tooth eruption 牙源性上皮细胞在牙萌期冠周组织中腱蛋白C表达的改变

IF 2.3 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-10-07 DOI: 10.1016/j.job.2025.100698

Kanako Suyama , Shohei Yoshimoto , Masahide Taguchi , Satoko Sumi , Tetsuya Kumagai , Kayoko Ogata , Shirabe Kurihara , Youichi Kuba , Kyoko Oka

Objectives

We aimed to elucidate the role of odontogenic epithelium in pericoronal tissue during tooth eruption by investigating the morphology of epithelial cells and expression of tenascin C.

Methods

Biopsy samples from human patients diagnosed with lower first molar eruption disorders, along with mandibular molar tissues from ICR mice, collected between postnatal day 10 (PN10) and PN15, were examined. Immunohistochemical analyses were performed to evaluate the expression of cytokeratin, tenascin C, E-cadherin, pSmads, and Ki67. In situ hybridization was used to detect expression of tenascin C mRNA.

Results

In human lower first molar specimens, a band-like configuration of epithelial islands was observed in the pericoronal connective tissue, with and without expression of tenascin C. In one patient, a high level of tenascin C mRNA was detected in the continuous epithelial layer covering the crown of impacted first molars, and the expression of tenascin C correlated with Ki67, a marker of cell proliferation. During lower molar tooth eruption in mice, odontogenic epithelial cells transformed from a mesh-like to a sheet-like configuration, accompanied by a reduction in the expression of tenascin C protein and mRNA. There was no proliferation or expression of pSmads in the epithelial component.

Conclusions

Persistent secretion of tenascin C by odontogenic epithelial cells may promote proliferation of the cells and increase accumulation of tenascin C in the pericoronal tissue. These findings suggest that sustained expression of tenascin C suppresses the progression of tooth eruption.

目的通过研究牙源性上皮细胞的形态和tenascin c的表达，阐明牙源性上皮在牙齿萌出过程中冠周组织中的作用。方法对诊断为下第一磨牙萌出障碍的人类患者和ICR小鼠在出生后10天（PN10）至15天之间采集的下颌磨牙组织进行检测。免疫组织化学分析评估细胞角蛋白、tenascin C、E-cadherin、pSmads和Ki67的表达。采用原位杂交法检测tenascin C mRNA的表达。结果在人类下第一磨牙标本中，冠状周结缔组织中可见带状上皮岛，有或没有tenascin C的表达。在1例患者中，在覆盖阻生第一磨牙冠的连续上皮层中检测到高水平的tenascin C mRNA， tenascin C的表达与细胞增殖标志物Ki67相关。在小鼠下磨牙萌出过程中，牙源性上皮细胞由网状结构转变为片状结构，并伴有腱蛋白C蛋白和mRNA表达的减少。上皮成分中没有pSmads的增殖和表达。结论牙源性上皮细胞持续分泌tenascin C可促进细胞增殖，增加冠状周组织tenascin C的积累。这些发现表明，持续表达tenascin C可以抑制牙齿萌牙的进展。

{"title":"Altered expression of tenascin C in pericoronal tissues by odontogenic epithelial cells during tooth eruption","authors":"Kanako Suyama , Shohei Yoshimoto , Masahide Taguchi , Satoko Sumi , Tetsuya Kumagai , Kayoko Ogata , Shirabe Kurihara , Youichi Kuba , Kyoko Oka","doi":"10.1016/j.job.2025.100698","DOIUrl":"10.1016/j.job.2025.100698","url":null,"abstract":"<div><h3>Objectives</h3><div>We aimed to elucidate the role of odontogenic epithelium in pericoronal tissue during tooth eruption by investigating the morphology of epithelial cells and expression of tenascin C.</div></div><div><h3>Methods</h3><div>Biopsy samples from human patients diagnosed with lower first molar eruption disorders, along with mandibular molar tissues from ICR mice, collected between postnatal day 10 (PN10) and PN15, were examined. Immunohistochemical analyses were performed to evaluate the expression of cytokeratin, tenascin C, E-cadherin, pSmads, and Ki67. <em>In situ</em> hybridization was used to detect expression of tenascin C mRNA.</div></div><div><h3>Results</h3><div>In human lower first molar specimens, a band-like configuration of epithelial islands was observed in the pericoronal connective tissue, with and without expression of tenascin C. In one patient, a high level of tenascin C mRNA was detected in the continuous epithelial layer covering the crown of impacted first molars, and the expression of tenascin C correlated with Ki67, a marker of cell proliferation. During lower molar tooth eruption in mice, odontogenic epithelial cells transformed from a mesh-like to a sheet-like configuration, accompanied by a reduction in the expression of tenascin C protein and mRNA. There was no proliferation or expression of pSmads in the epithelial component.</div></div><div><h3>Conclusions</h3><div>Persistent secretion of tenascin C by odontogenic epithelial cells may promote proliferation of the cells and increase accumulation of tenascin C in the pericoronal tissue. These findings suggest that sustained expression of tenascin C suppresses the progression of tooth eruption.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100698"},"PeriodicalIF":2.3,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145265862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanism of NF-κB-mediated transcriptional activation of MMP12 in lipopolysaccharide-stimulated human dental pulp stem cells 脂多糖刺激人牙髓干细胞中NF-κ b介导的MMP12转录激活的分子机制

IF 2.3 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-09-30 DOI: 10.1016/j.job.2025.100699

Yishu Xiao , Zhi Song , Derong Zeng , Yuhong Yang , Weiqi Peng , Qiaozhen Lin , Yun Huang , Wenxiang Chai , Yonghui Li , Xiu Zhao

Objectives

To investigate the molecular mechanisms through which matrix metalloprotein (MMP)12 exacerbates inflammation in pulpitis and to explore a potential regulatory axis between lipopolysaccharide (LPS)-induced MMP12 expression and activation of the nuclear factor-κB (NF-κB) signaling pathway.

Methods

An inflammatory pulpitis model was established using LPS-stimulated human dental pulp stem cells (hDPSCs). Transcriptomic profiling was conducted to identify differentially expressed genes between physiological and inflammatory states via RNA sequencing, with validation performed using quantitative real-time PCR (qPCR). The regulatory influence of the NF-κB pathway on MMP12 and cytokine expression was examined through western blotting and qPCR. In addition, dual-luciferase reporter assays were employed to confirm NF-κB binding motifs within the MMP12 promoter region.

Results

Treatment with LPS (1 μg/mL) triggered inflammatory activation in hDPSCs. Six pulpitis-associated genes, MMP12, IL6, IL8, IL10, IL1β, and TNF-α, were significantly upregulated (P < 0.05). Inhibition of NF-κB markedly reduced MMP12 expression. Moreover, NF-κB was found to transcriptionally activate the MMP12 promoter through binding motifs located at positions −1590/−1600 bp upstream, thereby establishing an NF-κB/MMP12 signaling axis.

Conclusion

The NF-κB/MMP12 axis functions as a critical amplifier of inflammation in pulpitis. The data shows that MMP12 is a major mediator of pulp tissue destruction and represents a potential therapeutic target, highlighting a coordinated mechanism underlying disease progression.

目的探讨基质金属蛋白（MMP）12加重牙髓炎炎症的分子机制，探讨脂多糖（LPS）诱导的MMP12表达与核因子-κB （NF-κB）信号通路激活之间的潜在调控轴。方法采用lps刺激的人牙髓干细胞（hDPSCs）建立炎症性牙髓炎模型。转录组学分析通过RNA测序鉴定生理状态和炎症状态之间的差异表达基因，并使用定量实时PCR （qPCR）进行验证。通过western blotting和qPCR检测NF-κB通路对MMP12和细胞因子表达的调控作用。此外，采用双荧光素酶报告基因检测来确认MMP12启动子区域内的NF-κB结合基序。结果LPS （1 μg/mL）可引起hdpsc的炎症活化。6个牙髓炎相关基因MMP12、IL6、IL8、IL10、IL1β和TNF-α显著上调（P < 0.05）。抑制NF-κB可显著降低MMP12的表达。此外，研究发现NF-κB通过结合上游- 1590/ - 1600 bp位置的基序转录激活MMP12启动子，从而建立NF-κB/MMP12信号转导轴。结论NF-κB/MMP12轴是牙髓炎炎症的关键放大因子。数据显示，MMP12是牙髓组织破坏的主要介质，代表了潜在的治疗靶点，突出了疾病进展的协调机制。

{"title":"Molecular mechanism of NF-κB-mediated transcriptional activation of MMP12 in lipopolysaccharide-stimulated human dental pulp stem cells","authors":"Yishu Xiao , Zhi Song , Derong Zeng , Yuhong Yang , Weiqi Peng , Qiaozhen Lin , Yun Huang , Wenxiang Chai , Yonghui Li , Xiu Zhao","doi":"10.1016/j.job.2025.100699","DOIUrl":"10.1016/j.job.2025.100699","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the molecular mechanisms through which matrix metalloprotein (MMP)12 exacerbates inflammation in pulpitis and to explore a potential regulatory axis between lipopolysaccharide (LPS)-induced MMP12 expression and activation of the nuclear factor-κB (NF-κB) signaling pathway.</div></div><div><h3>Methods</h3><div>An inflammatory pulpitis model was established using LPS-stimulated human dental pulp stem cells (hDPSCs). Transcriptomic profiling was conducted to identify differentially expressed genes between physiological and inflammatory states via RNA sequencing, with validation performed using quantitative real-time PCR (qPCR). The regulatory influence of the NF-κB pathway on MMP12 and cytokine expression was examined through western blotting and qPCR. In addition, dual-luciferase reporter assays were employed to confirm NF-κB binding motifs within the MMP12 promoter region.</div></div><div><h3>Results</h3><div>Treatment with LPS (1 μg/mL) triggered inflammatory activation in hDPSCs. Six pulpitis-associated genes, <em>MMP12, IL6, IL8, IL10, IL1β,</em> and <em>TNF-</em>α, were significantly upregulated (<em>P</em> < 0.05). Inhibition of NF-κB markedly reduced MMP12 expression. Moreover, NF-κB was found to transcriptionally activate the MMP12 promoter through binding motifs located at positions −1590/−1600 bp upstream, thereby establishing an NF-κB/MMP12 signaling axis.</div></div><div><h3>Conclusion</h3><div>The NF-κB/MMP12 axis functions as a critical amplifier of inflammation in pulpitis. The data shows that MMP12 is a major mediator of pulp tissue destruction and represents a potential therapeutic target, highlighting a coordinated mechanism underlying disease progression.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100699"},"PeriodicalIF":2.3,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localization of high endothelial venules is important for the prognosis of patients with oral squamous cell carcinoma 高内皮小静脉的定位对口腔鳞状细胞癌患者的预后很重要

IF 2.3 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-09-19 DOI: 10.1016/j.job.2025.100695

Takashi Niiyama , Aya Matsuda , Nako Maishi , Yasuhiro Hida , Alam Mohammad Towfik , Saki Shinohara , Amane Mizuno , Ren Zixiao , Lihong Lin , Wataru Kakuguchi , Kenichiro Sakata , Yoichi Ohiro , Yoshimasa Kitagawa , Hiroaki Suzuki , Michihiro Ueda , Kyoko Hida

Objectives

Surgery, chemotherapy, and radiation therapy are currently used for the treatment of malignant tumors, and immunotherapy has recently been established as the fourth method for treating cancer. Therefore, cancer cells and their surrounding microenvironments have been the focus of attention. High endothelial venules (HEVs) that mediate lymphocyte extravasation into lymphoid organs have been reported in cancerous tissues. However, the role of HEVs remains controversial. In this study, the clinical significance of HEVs in patients with oral squamous cell carcinoma (OSCC) was examined.

Methods

Eighty-three patients with OSCC of the tongue who had surgery as initial treatment were included. HEVs and lymphocytes were immunohistochemically stained, and their numbers and localization were evaluated.

Results

The prognosis significantly improved in patients with a high number of HEVs. Additionally, localization of HEVs to the tumor margins was associated with a good prognosis. Patients with localized infiltration of CD8-positive cells at the tumor margin had a significantly better prognosis, although no correlation was observed between the number of CD8-positive cells around the HEVs and the prognosis. Cox proportional hazards model revealed that TNM Stage, localization of HEVs, and localization of CD8-positive cells are prognostic factors affecting disease-free survival in patients with OSCC.

Conclusions

The localization of HEVs and CD8-positive cells affect the prognosis of patients with OSCC and they are beneficial prognostic factors.

目的目前恶性肿瘤的治疗方法主要有手术、化疗和放射治疗，而免疫治疗最近被确立为治疗癌症的第四大方法。因此，癌细胞及其周围微环境一直是人们关注的焦点。高内皮小静脉（HEVs）介导淋巴细胞外渗到淋巴器官已经报道在癌组织。然而，混合动力汽车的作用仍然存在争议。本研究旨在探讨口腔鳞状细胞癌（OSCC）患者体内hev的临床意义。方法对83例经手术治疗的舌鳞癌患者进行回顾性分析。免疫组织化学染色hev和淋巴细胞，评估其数量和定位。结果hev数量多的患者预后明显改善。此外，hev在肿瘤边缘的定位与良好的预后相关。肿瘤边缘有cd8阳性细胞局部浸润的患者预后明显较好，但hev周围cd8阳性细胞数量与预后无相关性。Cox比例风险模型显示，TNM分期、hev定位和cd8阳性细胞定位是影响OSCC患者无病生存的预后因素。结论hev和cd8阳性细胞的定位影响OSCC患者的预后，是有益的预后因素。

{"title":"Localization of high endothelial venules is important for the prognosis of patients with oral squamous cell carcinoma","authors":"Takashi Niiyama , Aya Matsuda , Nako Maishi , Yasuhiro Hida , Alam Mohammad Towfik , Saki Shinohara , Amane Mizuno , Ren Zixiao , Lihong Lin , Wataru Kakuguchi , Kenichiro Sakata , Yoichi Ohiro , Yoshimasa Kitagawa , Hiroaki Suzuki , Michihiro Ueda , Kyoko Hida","doi":"10.1016/j.job.2025.100695","DOIUrl":"10.1016/j.job.2025.100695","url":null,"abstract":"<div><h3>Objectives</h3><div>Surgery, chemotherapy, and radiation therapy are currently used for the treatment of malignant tumors, and immunotherapy has recently been established as the fourth method for treating cancer. Therefore, cancer cells and their surrounding microenvironments have been the focus of attention. High endothelial venules (HEVs) that mediate lymphocyte extravasation into lymphoid organs have been reported in cancerous tissues. However, the role of HEVs remains controversial. In this study, the clinical significance of HEVs in patients with oral squamous cell carcinoma (OSCC) was examined.</div></div><div><h3>Methods</h3><div>Eighty-three patients with OSCC of the tongue who had surgery as initial treatment were included. HEVs and lymphocytes were immunohistochemically stained, and their numbers and localization were evaluated.</div></div><div><h3>Results</h3><div>The prognosis significantly improved in patients with a high number of HEVs. Additionally, localization of HEVs to the tumor margins was associated with a good prognosis. Patients with localized infiltration of CD8-positive cells at the tumor margin had a significantly better prognosis, although no correlation was observed between the number of CD8-positive cells around the HEVs and the prognosis. Cox proportional hazards model revealed that TNM Stage, localization of HEVs, and localization of CD8-positive cells are prognostic factors affecting disease-free survival in patients with OSCC.</div></div><div><h3>Conclusions</h3><div>The localization of HEVs and CD8-positive cells affect the prognosis of patients with OSCC and they are beneficial prognostic factors.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100695"},"PeriodicalIF":2.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial profiling of bold green tea bottled beverages: A screening experiment 大胆的绿茶瓶装饮料的微生物分析：筛选实验

IF 2.3 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-09-17 DOI: 10.1016/j.job.2025.100697

Manami Imai , Miho Kawachi , Anna Wakui , Misato Miyazawa , Mirai Sekiguchi , Yuki Kato , Haruna Sato , Yuka Naruse , Nanami Asano , Momoko Morohashi , Hiroto Sano , Yuki Abiko , Jumpei Washio , Kaori Tanaka , Nobuhiro Takahashi , Takuichi Sato

Objectives

To explore the storage potential and drinking safety of leftover bottled tea beverages from various manufacturers after directly drinking from the bottle, we conducted a screening experiment on the growth of salivary bacteria in plastic bottles of bold green tea.

Methods

Resting whole saliva (n = 14) was collected from each participant (aged 19–25 years). The saliva samples (1.0 mL of each diluted saliva sample [5.0 × 10⁵ CFU/mL]), were inoculated into plastic bottles containing 280 mL of green tea, which included six types of bold green tea beverages. The bottles were stored at 37 °C for 24 h; subsequently, 1.0 mL of each sample was inoculated onto a blood agar plate and incubated anaerobically at 37 °C. Genomic DNA was extracted from the resulting individual colonies and the bacterial species were identified by 16S rDNA sequencing.

Results

More than 60 % of the samples of six types of bold green tea beverages, Bold Oi Ocha®, Suntory Bold Green Tea Iyemon®, Bold Oi Ocha Premium Strong®, Healthya®, Bold Ayataka®, and Catechin Green Tea® showed low bacterial levels (<10³ CFU/mL) after 1 day of storage. However, in some cases, former members of the genus Lactobacillus, such as Limosilactobacillus and Lactiplantibacillus spp., were specifically detected as the predominant bacteria (37.6–100 %), although these bacteria usually account for the minority among the oral microbiome.

Conclusions

Although the antibacterial effects of catechins may have affected the total bacterial counts and compositions, no clear correlation was observed between total tea catechin concentrations and total bacterial growth inhibitory effects.

目的探讨不同厂家的剩余瓶装茶饮料直接从瓶中饮用后的储存潜力和饮用安全性，对bold绿茶塑料瓶中唾液细菌的生长进行筛选实验。方法采集19 ~ 25岁参与者的静息全唾液（n = 14）。将唾液样本（每份稀释后的唾液样本1.0 mL [5.0 × 105 CFU/mL]）接种到含有280 mL绿茶的塑料瓶中，其中包括6种大胆的绿茶饮料。37℃保存24 h；随后，每种样品接种1.0 mL于血琼脂平板上，37℃厌氧培养。从得到的单个菌落中提取基因组DNA，并通过16S rDNA测序鉴定细菌种类。结果bold Oi Ocha®、三tory bold green tea Iyemon®、bold Oi Ocha Premium Strong®、Healthya®、bold Ayataka®和儿茶素绿茶®6种bold绿茶饮料在1天后的细菌含量均低于103 CFU/mL，超过60%。然而，在某些情况下，乳酸菌属的前成员，如Limosilactobacillus和Lactiplantibacillus spp.，被专门检测为优势菌（37.6 - 100%），尽管这些细菌通常在口腔微生物群中占少数。结论虽然儿茶素的抑菌作用可能影响细菌总数和组成，但儿茶素浓度与总细菌生长抑制作用之间没有明显的相关性。

{"title":"Microbial profiling of bold green tea bottled beverages: A screening experiment","authors":"Manami Imai , Miho Kawachi , Anna Wakui , Misato Miyazawa , Mirai Sekiguchi , Yuki Kato , Haruna Sato , Yuka Naruse , Nanami Asano , Momoko Morohashi , Hiroto Sano , Yuki Abiko , Jumpei Washio , Kaori Tanaka , Nobuhiro Takahashi , Takuichi Sato","doi":"10.1016/j.job.2025.100697","DOIUrl":"10.1016/j.job.2025.100697","url":null,"abstract":"<div><h3>Objectives</h3><div>To explore the storage potential and drinking safety of leftover bottled tea beverages from various manufacturers after directly drinking from the bottle, we conducted a screening experiment on the growth of salivary bacteria in plastic bottles of bold green tea.</div></div><div><h3>Methods</h3><div>Resting whole saliva (n = 14) was collected from each participant (aged 19–25 years). The saliva samples (1.0 mL of each diluted saliva sample [5.0 × 10<sup>5</sup> CFU/mL]), were inoculated into plastic bottles containing 280 mL of green tea, which included six types of bold green tea beverages. The bottles were stored at 37 °C for 24 h; subsequently, 1.0 mL of each sample was inoculated onto a blood agar plate and incubated anaerobically at 37 °C. Genomic DNA was extracted from the resulting individual colonies and the bacterial species were identified by 16S rDNA sequencing.</div></div><div><h3>Results</h3><div>More than 60 % of the samples of six types of bold green tea beverages, Bold Oi Ocha®, Suntory Bold Green Tea Iyemon®, Bold Oi Ocha Premium Strong®, Healthya®, Bold Ayataka®, and Catechin Green Tea® showed low bacterial levels (<10<sup>3</sup> CFU/mL) after 1 day of storage. However, in some cases, former members of the genus <em>Lactobacillus</em>, such as <em>Limosilactobacillus</em> and <em>Lactiplantibacillus</em> spp., were specifically detected as the predominant bacteria (37.6–100 %), although these bacteria usually account for the minority among the oral microbiome.</div></div><div><h3>Conclusions</h3><div>Although the antibacterial effects of catechins may have affected the total bacterial counts and compositions, no clear correlation was observed between total tea catechin concentrations and total bacterial growth inhibitory effects.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100697"},"PeriodicalIF":2.3,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural features of the articular disc of the rat temporomandibular joint: Fibrocartilage or dense fibrous lamina? 大鼠颞下颌关节盘的结构特征：纤维软骨还是致密纤维层？

IF 2.3 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-09-12 DOI: 10.1016/j.job.2025.100694

Shunichi Shibata , Masami Takahashi , Toru Shibui , Yuri Seki-Kishimoto , Masaki Takechi , Kazuharu Irie

Objectives

The structure of the temporomandibular joint (TMJ disc) articular disc as a dense fibrous lamina or fibrocartilage is an ongoing debate. To clarify this, we investigated TMJ disc cells in 3- to 20-week-old rats from the perspectives of general histology, ultrastructure, immunohistochemistry, and in situ hybridization.

Methods

Sections from paraffin-embedded TMJ discs were immunohistochemically stained using antibodies against aggrecan and collagen type II. Expression of Acan and Col2a1 mRNA was analyzed by in situ hybridization using antisense cRNA probes. Other samples were analyzed by transmission electron microscopy.

Results

Chondrocyte-like cells were visualized on the most medial aspect of the anterior band at 20 weeks. Transmission electron microscopy revealed that these cells were structurally similar to chondrocytes (termed “morphological chondrocytes”) but with less abundant rough endoplasmic reticulum and low expression of Acan and Col2a1 mRNA. Immunoreactivity for aggrecan and collagen type II was sparse in the extracellular matrix, but the areas of positivity were narrow. Immunoreactivity for hypoxia-inducible factor-1α was detected in the morphological chondrocytes and CD31-positive capillaries did not enter the disc, indicating an enhanced hypoxic environment in this region.

Conclusions

The presence of morphological chondrocytes in the articular disc region indicates an adaptation to the hypoxic environment rather than increased synthesis of cartilaginous matrix synthesis; hence, the rat TMJ disc can be regarded, in general, as a dense fibrous lamina rather than consisting of fibrocartilage.

目的颞下颌关节盘的结构是致密纤维层还是纤维软骨一直是一个争论不休的问题。为了阐明这一点，我们从一般组织学、超微结构、免疫组织化学和原位杂交的角度研究了3 ~ 20周龄大鼠的TMJ椎间盘细胞。方法采用免疫组织化学方法对石蜡包埋的TMJ椎间盘切片进行抗聚集蛋白和II型胶原抗体染色。利用反义cRNA探针原位杂交分析Acan和Col2a1 mRNA的表达。其他样品用透射电镜分析。结果20周时，前腱束最内侧可见软骨细胞样细胞。透射电镜显示，这些细胞在结构上与软骨细胞相似（称为“形态软骨细胞”），但粗糙的内质网较少，Acan和Col2a1 mRNA的表达较低。细胞外基质中聚集蛋白和II型胶原蛋白的免疫反应性稀疏，阳性区域狭窄。形态学软骨细胞中检测到缺氧诱导因子-1α的免疫反应性，cd31阳性的毛细血管未进入椎间盘，表明该区域缺氧环境增强。结论关节盘区形态软骨细胞的出现表明其对缺氧环境的适应，而不是软骨基质合成的增加；因此，大鼠TMJ椎间盘一般可视为致密的纤维层，而不是由纤维软骨组成。

{"title":"Structural features of the articular disc of the rat temporomandibular joint: Fibrocartilage or dense fibrous lamina?","authors":"Shunichi Shibata , Masami Takahashi , Toru Shibui , Yuri Seki-Kishimoto , Masaki Takechi , Kazuharu Irie","doi":"10.1016/j.job.2025.100694","DOIUrl":"10.1016/j.job.2025.100694","url":null,"abstract":"<div><h3>Objectives</h3><div>The structure of the temporomandibular joint (TMJ disc) articular disc as a dense fibrous lamina or fibrocartilage is an ongoing debate. To clarify this, we investigated TMJ disc cells in 3- to 20-week-old rats from the perspectives of general histology, ultrastructure, immunohistochemistry, and <em>in situ</em> hybridization.</div></div><div><h3>Methods</h3><div>Sections from paraffin-embedded TMJ discs were immunohistochemically stained using antibodies against aggrecan and collagen type II. Expression of <em>Acan</em> and <em>Col2a1</em> mRNA was analyzed by <em>in situ</em> hybridization using antisense cRNA probes. Other samples were analyzed by transmission electron microscopy.</div></div><div><h3>Results</h3><div>Chondrocyte-like cells were visualized on the most medial aspect of the anterior band at 20 weeks. Transmission electron microscopy revealed that these cells were structurally similar to chondrocytes (termed “morphological chondrocytes”) but with less abundant rough endoplasmic reticulum and low expression of <em>Acan</em> and <em>Col2a1</em> mRNA. Immunoreactivity for aggrecan and collagen type II was sparse in the extracellular matrix, but the areas of positivity were narrow. Immunoreactivity for hypoxia-inducible factor-1α was detected in the morphological chondrocytes and CD31-positive capillaries did not enter the disc, indicating an enhanced hypoxic environment in this region.</div></div><div><h3>Conclusions</h3><div>The presence of morphological chondrocytes in the articular disc region indicates an adaptation to the hypoxic environment rather than increased synthesis of cartilaginous matrix synthesis; hence, the rat TMJ disc can be regarded, in general, as a dense fibrous lamina rather than consisting of fibrocartilage.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100694"},"PeriodicalIF":2.3,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep learning approaches for pathological image classification 病理图像分类的深度学习方法

IF 2.3 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-09-08 DOI: 10.1016/j.job.2025.100696

Masayuki Tsuneki

Background

Computer-aided diagnosis using deep learning has emerged as a transformative tool in pathology that enables the automated and consistent interpretation of whole slide images. Among the key tasks in pathological image analysis, classification-based deep learning models have shown promise in distinguishing cancer subtypes and predicting genetic or molecular features. However, challenges remain due to the limited availability of high-quality labeled datasets, particularly for rare cancers, which hinders the widespread applicability of conventional data-driven approaches.

Highlight

The methodology for developing classification-based deep learning models involves data preparation, annotation strategies, training techniques, and performance evaluation. Core supervised learning approaches are used alongside convolutional and recurrent neural networks to enhance classification performance. In scenarios in which training datasets are scarce, transfer learning serves as an effective strategy to improve the model training efficiency. In addition, this review introduces emerging techniques such as the use of simulators to generate synthetic data and formula-driven approaches that simulate realistic scenarios, with the aim of overcoming the limitations of conventional training datasets. Visualization tools such as probability heatmaps are critical for model interpretability and validation.

Conclusion

Classification-based deep learning models are expected to play an increasing role in precision medicine by expanding capabilities beyond diagnosis to include prognostic prediction and treatment decision making. With innovations in augmented intelligence, deep learning models can comprehensively support clinicians across the diagnostic and therapeutic spectrum, particularly in an era of increasing demand and limited human resources in pathology.

使用深度学习的计算机辅助诊断已经成为病理学中的一种变革性工具，可以对整个幻灯片图像进行自动化和一致的解释。在病理图像分析的关键任务中，基于分类的深度学习模型在区分癌症亚型和预测遗传或分子特征方面显示出了希望。然而，由于高质量标记数据集的可用性有限，特别是对于罕见癌症，挑战仍然存在，这阻碍了传统数据驱动方法的广泛适用性。开发基于分类的深度学习模型的方法包括数据准备、注释策略、训练技术和性能评估。核心监督学习方法与卷积和循环神经网络一起使用，以提高分类性能。在训练数据稀缺的情况下，迁移学习是提高模型训练效率的有效策略。此外，本综述还介绍了新兴技术，如使用模拟器生成合成数据和公式驱动的方法来模拟现实场景，目的是克服传统训练数据集的局限性。像概率热图这样的可视化工具对于模型的可解释性和验证是至关重要的。结论基于分类的深度学习模型有望在精准医疗中发挥越来越大的作用，将其功能从诊断扩展到预后预测和治疗决策。随着增强智能的创新，深度学习模型可以在诊断和治疗范围内全面支持临床医生，特别是在病理学需求不断增长而人力资源有限的时代。

{"title":"Deep learning approaches for pathological image classification","authors":"Masayuki Tsuneki","doi":"10.1016/j.job.2025.100696","DOIUrl":"10.1016/j.job.2025.100696","url":null,"abstract":"<div><h3>Background</h3><div>Computer-aided diagnosis using deep learning has emerged as a transformative tool in pathology that enables the automated and consistent interpretation of whole slide images. Among the key tasks in pathological image analysis, classification-based deep learning models have shown promise in distinguishing cancer subtypes and predicting genetic or molecular features. However, challenges remain due to the limited availability of high-quality labeled datasets, particularly for rare cancers, which hinders the widespread applicability of conventional data-driven approaches.</div></div><div><h3>Highlight</h3><div>The methodology for developing classification-based deep learning models involves data preparation, annotation strategies, training techniques, and performance evaluation. Core supervised learning approaches are used alongside convolutional and recurrent neural networks to enhance classification performance. In scenarios in which training datasets are scarce, transfer learning serves as an effective strategy to improve the model training efficiency. In addition, this review introduces emerging techniques such as the use of simulators to generate synthetic data and formula-driven approaches that simulate realistic scenarios, with the aim of overcoming the limitations of conventional training datasets. Visualization tools such as probability heatmaps are critical for model interpretability and validation.</div></div><div><h3>Conclusion</h3><div>Classification-based deep learning models are expected to play an increasing role in precision medicine by expanding capabilities beyond diagnosis to include prognostic prediction and treatment decision making. With innovations in augmented intelligence, deep learning models can comprehensively support clinicians across the diagnostic and therapeutic spectrum, particularly in an era of increasing demand and limited human resources in pathology.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100696"},"PeriodicalIF":2.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges and prospects for popularizing cell therapy in the dentistry in Japan 细胞疗法在日本牙科推广的挑战与前景

IF 2.3 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-09-02 DOI: 10.1016/j.job.2025.100692

Morikuni Tobita, Anna Arita

Background

Regenerative medicine is actively used in dentistry in Japan and mesenchymal stromal cell (MSC) therapy shows promising potential. Despite the growing interest and some clinical and private practice applications, the adoption of MSC-based therapies remains limited. In this study, we report the current status of cell therapy in dentistry in Japan and discuss the challenges in popularizing cell therapy and the future direction of dental regenerative medicine.

Highlights

As of May 2025, MSCs were being used for oral tissue regeneration in clinical research and private practice. Dental pulp is the primary source of MSCs, followed by adipose tissue and bone marrow. Approximately 50 private practice dental clinics in Japan offer MSC-based therapies, with most outsourcing cell processing. Current challenges in ensuring the safety and validity of cell therapy in dentistry are: 1) limited verification of safety and efficacy for specific diseases before offering private practice and 2) lack of appropriately collected clinical data to support evidence-based practice.

Conclusion

To safely expand regenerative medicine in dentistry, stronger support for dental clinics is essential. This includes closer collaboration with academic institutions and the systematic collection of clinical data to ensure safety and efficacy.

在日本，再生医学在牙科中得到了积极的应用，间充质间质细胞（MSC）治疗显示出良好的潜力。尽管越来越多的兴趣和一些临床和私人实践应用，采用msc为基础的疗法仍然有限。在本研究中，我们报告了日本牙科细胞治疗的现状，并讨论了普及细胞治疗的挑战和牙科再生医学的未来方向。截至2025年5月，MSCs已被用于口腔组织再生的临床研究和私人实践。牙髓是骨髓间充质干细胞的主要来源，其次是脂肪组织和骨髓。日本大约有50家私人牙科诊所提供基于msc的治疗，大多数外包细胞处理。目前在确保牙科细胞治疗的安全性和有效性方面面临的挑战是：1)在提供私人诊所之前，对特定疾病的安全性和有效性的验证有限；2)缺乏适当收集的临床数据来支持循证实践。结论要安全推广牙科再生医学，必须加强对牙科诊所的支持。这包括与学术机构进行更密切的合作，并系统地收集临床数据，以确保安全性和有效性。

{"title":"Challenges and prospects for popularizing cell therapy in the dentistry in Japan","authors":"Morikuni Tobita, Anna Arita","doi":"10.1016/j.job.2025.100692","DOIUrl":"10.1016/j.job.2025.100692","url":null,"abstract":"<div><h3>Background</h3><div>Regenerative medicine is actively used in dentistry in Japan and mesenchymal stromal cell (MSC) therapy shows promising potential. Despite the growing interest and some clinical and private practice applications, the adoption of MSC-based therapies remains limited. In this study, we report the current status of cell therapy in dentistry in Japan and discuss the challenges in popularizing cell therapy and the future direction of dental regenerative medicine.</div></div><div><h3>Highlights</h3><div>As of May 2025, MSCs were being used for oral tissue regeneration in clinical research and private practice. Dental pulp is the primary source of MSCs, followed by adipose tissue and bone marrow. Approximately 50 private practice dental clinics in Japan offer MSC-based therapies, with most outsourcing cell processing. Current challenges in ensuring the safety and validity of cell therapy in dentistry are: 1) limited verification of safety and efficacy for specific diseases before offering private practice and 2) lack of appropriately collected clinical data to support evidence-based practice.</div></div><div><h3>Conclusion</h3><div>To safely expand regenerative medicine in dentistry, stronger support for dental clinics is essential. This includes closer collaboration with academic institutions and the systematic collection of clinical data to ensure safety and efficacy.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 4","pages":"Article 100692"},"PeriodicalIF":2.3,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0