Journal of Oral Biosciences最新文献_第2页

ED-71 promotes osseointegration of titanium implants in a rat model of GIOP by alleviating the effects of dexamethasone on bone remodeling in a SIRT1-dependent manner ED-71 可通过 SIRT1 依赖性方式减轻地塞米松对骨重塑的影响，从而促进钛植入物在大鼠 GIOP 模型中的骨结合。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.10.003

Chunying Li , Pengfei Xue , Guanglin Duan , Ailing Song , Runbing Zhai , Jie Ma , Minqi Li

Objective

Glucocorticoid-induced osteoporosis (GIOP), a common complication of glucocorticoid usage, plays a critical role in the success of dental implant restoration by affecting osseointegration. Eldecalcitol (ED-71) prevents GIOP; however, its role in the osseointegration of implants under GIOP conditions remains elusive.

Methods

Dexamethasone was used to establish a rat model of GIOP. Subsequently, mini-implant surgery was performed on the femur. GIOP rats were administered ED-71 via gavage to assess its role in the osseointegration of titanium implants under GIOP conditions. MC3T3-E1 and RAW264.7 cells were utilized to explore the molecular mechanism of ED-71 in ameliorating disorder of bone remodeling caused by dexamethasone.

Results

The administration of ED-71 promoted the formation of newly formed woven bone and the resolution of inflammation around titanium implants. In vitro experiments indicated that ED-71 ameliorated dexamethasone-induced dysfunction of osteoblasts and osteoclasts by increasing the expression level of sirtuin 1 (SIRT1). Inhibition of SIRT1 by selisistat counteracts the regulatory effects of ED-71 on dexamethasone-induced disorder of bone remodeling. Molecular docking and Western blotting revealed that the neurogenic locus notch homolog protein and nuclear factor kappa B signaling pathways are essential for the effects of ED-71 on dexamethasone-induced disorder of bone remodeling.

Conclusion

ED-71 promoted implant osseointegration in a rat model of GIOP by alleviating the effects of dexamethasone on bone remodeling in a SIRT1-dependent manner.

目的：糖皮质激素诱导的骨质疏松症（GIOP）是使用糖皮质激素的常见并发症，它通过影响骨结合对牙科种植修复的成功起着至关重要的作用。艾地卡骨化醇（ED-71）可预防 GIOP，但它在 GIOP 条件下对种植体骨结合的作用仍不明确：方法：使用地塞米松建立大鼠 GIOP 模型。方法：使用地塞米松建立 GIOP 大鼠模型，随后在股骨上进行微型植入手术。给 GIOP 大鼠灌胃 ED-71，以评估其在 GIOP 条件下对钛植入物骨结合的作用。利用 MC3T3-E1 和 RAW264.7 细胞探讨 ED-71 改善地塞米松引起的骨重塑紊乱的分子机制：结果：服用ED-71可促进新形成的编织骨的形成，并缓解钛种植体周围的炎症。体外实验表明，ED-71通过提高sirtuin 1（SIRT1）的表达水平，改善了地塞米松诱导的成骨细胞和破骨细胞功能障碍。塞利司他对SIRT1的抑制抵消了ED-71对地塞米松诱导的骨重塑障碍的调节作用。分子对接和Western印迹显示，ED-71对地塞米松诱导的骨重塑障碍的影响离不开神经原位点缺口同源蛋白和核因子卡巴B信号通路：结论：ED-71以SIRT1依赖的方式减轻了地塞米松对骨重塑的影响，从而促进了大鼠GIOP模型中种植体的骨结合。

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引用次数: 0

MANDI-code: A coding system for the human mandible MANDI-Code：人类下颌骨编码系统。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100584

Doha Abualhija , Julieta Gómez García-Donas , Simon Shepherd , Ranya Al Ghazi , Scheila Manica

Background

The mandible provides valuable insights into its biological identity. However, the existence of several terminologies for mandibular measurements and inconsistent language can lead to misinterpretation, confusion, and miscommunication.

Highlight

A standardised set of anatomical points, planes, and measurements would assist with these issues and ensure reproducibility and comparability.

Conclusion

The proposed coding system offers a comprehensive approach for professionals and researchers in dentistry, archaeology, and anthropology.

背景：下颌骨为了解生物特征提供了宝贵的信息。然而，下颌骨测量存在多种术语和不一致的语言，可能导致误解、混淆和误传：重点：一套标准化的解剖点、平面和测量方法将有助于解决这些问题，并确保可重复性和可比性：建议的编码系统为牙科、考古学和人类学领域的专业人员和研究人员提供了一种全面的方法。

引用次数: 0

Conditional heterozygous loss of kit receptor tyrosine kinase in neural crest cell lineage is associated with midline cleft lip and bifid nose deformity 神经嵴细胞系中Kit受体酪氨酸激酶的条件性杂合性缺失与中线唇裂和双劈鼻畸形有关。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.10.004

Hitomi Aoki , Hiroyuki Tomita , Akira Hara , Takahiro Kunisada

Objectives

The receptor tyrosine kinase Kit is expressed in cells derived from the trunk neural crest (NC), such as melanocytes; however, its role in cranial NC cell development is not fully understood.

Methods

We investigated the effects of the heterozygous loss of Kit in NC cells during embryonic development by mating Kit^2lox/+ mice with Wnt1-Cre mice to produce Wnt1-Cre; Kit^2lox/+ embryos. In addition, Wnt1-Cre mice were mated with Rosa26R-yellow fluorescent protein (YFP) mice to visualize the tissue regions expressing Cre recombinase. Histological studies of the craniofacial regions of these mice were performed using samples from embryonic day (E) 12.5 and postnatal day (P) 1. Cellular apoptosis and proliferation were both analyzed through the immunostaining of tissue sections collected on E13.5 and E14.5 using anti-cleaved caspase 3 (CC3) to detect apoptosis and anti-Ki67 to detect proliferation. Cells from YFP-positive tissue regions of the facial areas of Wnt1-Cre; Kit^+/+; Rosa26R–YFP embryos and Wnt1-Cre; Kit^2lox/+; Rosa26R–YFP embryos collected on E12.5 and E15.5 were cultured and evaluated for cell proliferation.

Results

Compared with control littermates, Wnt1-Cre; Kit^2lox/+ embryos exhibited midline cleft lip and bifid nose deformities. Substantial early (P1) postnatal lethality was observed in Wnt1-Cre; Kit^2lox/+ mice, with none surviving to 3 weeks of age. YFP-positive cells from the maxillary regions of Wnt1-Cre; Kit^2lox/+; Rosa26R–YFP embryos exhibited defective cell growth and self-renewal in vitro.

Conclusion

Conditional heterozygous loss of Kit in Wnt1-Cre; Kit^2lox/+ embryos is associated with craniofacial dysplasia and exhibit defective NC development in vitro and in vivo.

目的：受体酪氨酸激酶Kit在躯干神经嵴（NC）细胞（如黑色素细胞）中表达，但它在颅内NC细胞发育过程中的作用尚未完全清楚：受体酪氨酸激酶Kit在来自躯干神经嵴（NC）的细胞（如黑色素细胞）中表达，但它在颅骨NC细胞发育中的作用尚未完全清楚：方法：我们通过将Kit2lox/+小鼠与Wnt1-Cre小鼠交配产生Wnt1-Cre; Kit2lox/+胚胎，研究了在胚胎发育过程中NC细胞中杂合子Kit缺失的影响。此外，Wnt1-Cre小鼠还与Rosa26R-黄色荧光蛋白（YFP）小鼠交配，以观察表达Cre重组酶的组织区域。利用胚胎 12.5 天和出生后第 1 天的样本对这些小鼠的颅面部区域进行了组织学研究。通过对 E13.5 和 E14.5 采集的组织切片进行免疫染色来分析细胞的凋亡和增殖，使用抗裂解的 Caspase 3 (CC3) 来检测细胞凋亡，使用抗 Ki67 来检测细胞增殖。对E12.5和E15.5收集的Wnt1-Cre; Kit+/+; Rosa26R-YFP胚胎和Wnt1-Cre; Kit2lox/+; Rosa26R-YFP胚胎面部YFP阳性组织区的细胞进行培养并评估细胞增殖情况：结果：与对照同窝胚胎相比，Wnt1-Cre; Kit2lox/+胚胎表现出中线唇裂和双峰鼻畸形。Wnt1-Cre; Kit2lox/+小鼠出生后早期（P1）出现大量死亡，无一存活至3周大。来自 Wnt1-Cre; Kit2lox/+; Rosa26R-YFP 胚胎上颌骨区域的 YFP 阳性细胞在体外表现出细胞生长和自我更新缺陷：结论：Wnt1-Cre; Kit2lox/+ 胚胎中Kit的条件性杂合缺失与颅面发育不良有关，并表现出体外和体内NC发育缺陷。

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引用次数: 0

Remineralizing capacity of zinc oxide eugenol sealer following the addition of nanohydroxyapatite-tyrosine amino acid: An in vivo animal study 添加纳米羟基磷灰石-酪氨酸氨基酸后氧化锌丁香酚封闭剂的再矿化能力：活体动物研究。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.09.006

Rasha M. Al-Shamaa, Raghad A. Al-Askary

Objective

Although several sealers have been developed, the zinc oxide eugenol (ZOE) sealer is still used in many private dental clinics. This study aimed to compare the biocompatibility and remineralizing capacity οf ZOE sealer with the addition of nanohydroxyapatite-tyrosine amino acid.

Methods

This study was conducted on Twenty rabbits. The rabbits were divided into four groups based on the test observation period (3, 7, 21, and 28 days) following surgical implantation. General anesthesia was administered to each rabbit, and a subcutaneous incision of approximately 1 ± 0.5 cm was made along the symphyseal area of the mandible of each rabbit. Four bone cavities were generated in the interdental space of the lower jaw between the central and molar teeth of each rabbit, with one longitudinal subcutaneous incision. The ZOE sealers were mixed according to the manufacturer's guidelines and directly inserted within the cavities generated at the end of each test period. The animals were sacrificed, and bone biopsy was carried out at the site of testing. The biopsy samples were obtained and subjected to histological analysis using a low-power light microscope (Olympus C ⅹ 21, Japan) and immunohistochemistry using Ki67 antibody.

Results

The collected information was examined by parametric statistical tests using SPSS software version ‘‘22.” One-way ANOVA and post-hoc Duncan's tests were used to measure the significance among various groups, with statistical significance set, when “P ≤ 0.01”.

Conclusion

The 20% mixed endodontic sealer displayed excellent outcomes compared to other experimental groups, as identified by higher new bone formation at every evaluation period.

目的：尽管已开发出多种封闭剂，但许多私人牙科诊所仍在使用氧化锌丁香酚（ZOE）封闭剂。本研究旨在比较添加纳米羟基磷灰石-酪氨酸氨基酸的氧化锌封闭剂的生物相容性和再矿化能力：本研究以 20 只兔子为对象。根据手术植入后的试验观察期（3、7、21 和 28 天）将兔子分为四组。对每只兔子进行全身麻醉，沿每只兔子下颌骨的骨骺区做一个约 1±0.5 厘米的皮下切口。在每只兔子下颌中牙和磨牙之间的牙间隙生成四个骨腔，其中一个为纵向皮下切口。按照制造商的指导原则混合 ZOE 密封剂，并在每个测试期结束时直接将其插入生成的骨腔中。动物被处死，并在测试部位进行骨活检。获得活检样本后，使用低倍光学显微镜（日本奥林巴斯 C ⅹ 21）进行组织学分析，并使用 Ki67 抗体进行免疫组化：使用 SPSS 软件版本''22 "对收集的信息进行参数统计检验。采用单因素方差分析和事后邓肯检验来衡量各组间的显著性，当 "P≤0.01 "时，统计学意义成立：结论：与其他实验组相比，20% 混合根管封闭剂的效果非常好，在每个评估阶段都有较高的新骨形成。

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引用次数: 0

Impact of organic, conventional, and stingless bee honeys on the antibacterial activity of gummy candies against oral bacteria 有机蜂蜜、传统蜂蜜和无刺蜂蜂蜜对口腔细菌软糖抗菌活性的影响。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100589

José Renato Silva , Jerônimo Kahn Villas-Bôas , Guilherme Biz , Ricardo Sergio Couto Almeida , Wilma Spinosa , Sandra Helena Prudencio

Objectives

This study aimed to investigate the impact of organic, conventional, and stingless honey on gummy candies, focusing on the effect of the cariogenic bacterium, Streptococcus mutans UA159, and total bacterial count in saliva from adolescents.

Methods

Antimicrobial compounds in three honey samples were identified, and the minimum inhibitory concentration against S. mutans UA159 was determined. The antibacterial activities of the three honey candy formulations were determined against S. mutans UA159 in artificial saliva and total bacteria in saliva collected from adolescents. The sensory acceptance of the candy formulations by children, adolescents, and adults was investigated.

Results

Candies prepared using conventional honey showed the highest antibacterial activity against S. mutans UA159 in vitro and total bacteria in human saliva. This effect was attributed to the higher levels of quercetin, myricetin, caffeine, and hydrogen peroxide in conventional honey.

Conclusions

Nicotinic, ferulic, and p-coumaric acids found in honey had low antibacterial activity against oral bacteria. Quercetin, myricetin, caffeine, and hydrogen peroxide are the main anticariogenic compounds in honey and exert antibacterial effects on adolescent saliva, despite added to candies. However, organic production does not necessarily improve the biological properties of honey. All candies were equally liked by sensory assessors (acceptance >70%), facilitating the selection of honey with higher biological activities to formulate functional candies.

研究目的本研究旨在调查有机蜂蜜、传统蜂蜜和无刺蜂蜜对软糖的影响，重点是对致癌细菌变异链球菌 UA159 和青少年唾液中细菌总数的影响：方法：对三种蜂蜜样品中的抗菌化合物进行了鉴定，并测定了其对变异链球菌 UA159 的最小抑菌浓度。测定了三种蜂蜜糖果配方对人工唾液中的变异杆菌 UA159 和青少年唾液中细菌总数的抗菌活性。还调查了儿童、青少年和成人对糖果配方的感官接受度：结果：使用传统蜂蜜制备的糖果在体外对变异杆菌 UA159 和人体唾液中细菌总数的抗菌活性最高。这种效果归因于传统蜂蜜中较高含量的槲皮素、杨梅素、咖啡因和过氧化氢：结论：蜂蜜中的烟酸、阿魏酸和对香豆酸对口腔细菌的抗菌活性较低。槲皮素、杨梅素、咖啡因和过氧化氢是蜂蜜中主要的抗龋齿化合物，即使添加到糖果中，也能对青少年唾液产生抗菌作用。然而，有机生产并不一定能改善蜂蜜的生物特性。所有糖果都同样受到感官评估师的喜爱（接受度大于 70%），这有助于选择生物活性更高的蜂蜜来配制功能性糖果。

{"title":"Impact of organic, conventional, and stingless bee honeys on the antibacterial activity of gummy candies against oral bacteria","authors":"José Renato Silva , Jerônimo Kahn Villas-Bôas , Guilherme Biz , Ricardo Sergio Couto Almeida , Wilma Spinosa , Sandra Helena Prudencio","doi":"10.1016/j.job.2024.100589","DOIUrl":"10.1016/j.job.2024.100589","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to investigate the impact of organic, conventional, and stingless honey on gummy candies, focusing on the effect of the cariogenic bacterium, <em>Streptococcus mutans</em> UA159, and total bacterial count in saliva from adolescents.</div></div><div><h3>Methods</h3><div>Antimicrobial compounds in three honey samples were identified, and the minimum inhibitory concentration against <em>S. mutans</em> UA159 was determined. The antibacterial activities of the three honey candy formulations were determined against <em>S. mutans</em> UA159 in artificial saliva and total bacteria in saliva collected from adolescents. The sensory acceptance of the candy formulations by children, adolescents, and adults was investigated.</div></div><div><h3>Results</h3><div>Candies prepared using conventional honey showed the highest antibacterial activity against <em>S. mutans</em> UA159 <em>in vitro</em> and total bacteria in human saliva. This effect was attributed to the higher levels of quercetin, myricetin, caffeine, and hydrogen peroxide in conventional honey.</div></div><div><h3>Conclusions</h3><div>Nicotinic, ferulic, and <em>p</em>-coumaric acids found in honey had low antibacterial activity against oral bacteria. Quercetin, myricetin, caffeine, and hydrogen peroxide are the main anticariogenic compounds in honey and exert antibacterial effects on adolescent saliva, despite added to candies. However, organic production does not necessarily improve the biological properties of honey. All candies were equally liked by sensory assessors (acceptance >70%), facilitating the selection of honey with higher biological activities to formulate functional candies.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 1","pages":"Article 100589"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142689164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maxillary sinus floor augmentation using sponge- and cotton-like graft materials in a rabbit model 在兔子模型中使用海绵和棉花状移植材料进行上颌窦底隆起。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100586

Seigo Ohba , Rena Shido , Hideyuki Yamamoto , Masahito Hara , Yasutoshi Nishikawa , Toshihiro Kasuga , Tomohiro Yamada , Yoshinori Sumita , Tatsuo Shirota

Objectives

Bone graft materials commonly used for maxillary sinus floor augmentation (MSFA), including hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP), are mostly granular and have poor handleability. HAp/collagen composite material (HAp/Col) and β-tricalcium phosphate (β-TCP)/poly(L-lactide-co-glycolide) (PLGA) have shown promise but their application in MSFA as bone graft materials remains unclear. Here, we investigated the bone-forming behavior of HAp/Col and β-TCP/PLGA in an MSFA rabbit model.

Methods

Male Japanese white rabbits were used. HAP/Col or β-TCP/PLGA was randomly applied to the MSFA model. The specimens were harvested at 4 weeks (W), 8W, 16W, and 24W after surgery, and the augmented regions were evaluated using micro-computed tomography and histological analyses.

Results

The graft materials were retained up to 16W in the HAp/Col group and 24W in the β-TCP/PLGA group. The augmented volume detected in the HAp/Col group at 4W was substantially reduced at subsequent time points. However, in the β-TCP/PLGA group, the volume observed at 4W was maintained up to 24W. In the HAp/Col group, the bone mineral content (BMC) at 4W was significantly lower than that at 8W (p = 0.03716), and this elevated BMC was significantly decreased at 16W (p = 0.00185) and 24W (p = 0.00236). In the β-TCP/PLGA group, the BMC tended to increase from 4W to 16W and then decreased.

Conclusions

Both HAp/Col and β-TCP/PLGA are useful for MSFA because of their ability to form new bone and good handleability. The appropriate graft material should be selected depending on the application needs while understanding the properties of the newly formed bone.

目的：常用于上颌窦底增量术（MSFA）的骨移植材料，包括羟基磷灰石（HAp）和β-磷酸三钙（β-TCP），大多呈颗粒状，可操作性差。HAp/胶原复合材料（HAp/Col）和β-磷酸三钙（β-TCP）/聚（L-乳酸-聚乙二醇）（PLGA）已显示出应用前景，但它们作为骨移植材料在 MSFA 中的应用仍不明确。在此，我们研究了 HAp/Col 和 β-TCP/PLGA 在 MSFA 兔子模型中的骨形成行为：方法：使用雄性日本白兔。方法：使用雄性日本白兔，将 HAP/Col 或 β-TCP/PLGA 随机应用于 MSFA 模型。分别于术后 4 周、8 周、16 周和 24 周采集标本，并使用显微计算机断层扫描和组织学分析对增殖区域进行评估：结果：HAp/Col 组和 β-TCP/PLGA 组的移植材料保留时间分别为 16W 和 24W。HAp/Col 组在 4W 时检测到的增量在随后的时间点大幅减少。但在β-TCP/PLGA组，4W时观察到的体积一直保持到24W。在 HAp/Col 组中，4W 时的骨矿物质含量（BMC）显著低于 8W 时（p=0.03716），这种升高的 BMC 在 16W 时（p=0.00185）和 24W 时（p=0.00236）显著下降。在β-TCP/PLGA组，BMC从4W到16W呈上升趋势，然后下降：结论：HAp/Col 和 β-TCP/PLGA 均可用于 MSFA，因为它们具有形成新骨的能力和良好的可操作性。应根据应用需要选择合适的移植材料，同时了解新形成骨的特性。

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引用次数: 0

CCN2: A potential contributor to gingival overgrowth CCN2：牙龈过度生长的潜在因素。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100587

Asmaa Fadl, Andrew Leask

Background

Fibrotic responses in the gingiva are characterized by their hyperproliferative nature instead of scar tissue formation. Clinically, these conditions appear as “gingival overgrowth” (GO), which can be of drug-induced or genetic origin. Despite surgical removal, GO can recur. Therefore, non-invasive methods of treating GO are required. In other fibrotic systems, the matricellular protein CCN2 represents a potential therapeutic target. However, CCN2 has been relatively understudied in the context of GO.

Highlight

Herein, we describe what is known regarding CCN2 expression in GO and gingival fibroblasts. Specifically, CCN2 is induced by agents that promote fibrogenesis in the oral cavity, such as transforming growth factor−β, and drugs that promote GO, such as cyclosporine, nifedipine, and phenytoin.

Conclusion

Although little is known regarding the possible function of CCN2 in GO, given the correlation between CCN2 expression and GO recurrence, we hope that this review will inspire further research on this topic.

背景：牙龈纤维化反应的特点是过度增生，而不是瘢痕组织的形成。在临床上，这些情况表现为 "牙龈增生"（Gingival overgrowth，GO），可能由药物或遗传因素引起。尽管可以通过手术切除，但牙龈增生仍有可能复发。因此，需要采用非侵入性的方法来治疗牙龈增生。在其他纤维化系统中，基质细胞蛋白 CCN2 是一个潜在的治疗靶点。亮点：在此，我们描述了目前已知的有关 CCN2 在牙龈组织和牙龈成纤维细胞中的表达情况。特别是，促进口腔纤维生成的药物（如转化生长因子-β）和促进GO的药物（如环孢素、硝苯地平和苯妥英）会诱导CCN2：尽管对 CCN2 在口腔溃疡中的可能功能知之甚少，但考虑到 CCN2 的表达与口腔溃疡复发之间的相关性，我们希望这篇综述能激发对这一主题的进一步研究。

引用次数: 0

Exploring the mechanism of tiRNA-Val-CAC-002 in the pathogenesis of oral submucous fibrosis 探索 tiRNA-Val-CAC-002 在口腔黏膜下纤维化发病机制中的作用机制

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100588

Liujun Zeng , Hui Peng , Leiye Sun , Huiqiao Yu , Yingfang Wu

Objectives

Oral submucous fibrosis (OSF) is a chronic, progressive, and potentially malignant disease of the oral cavity. A previous study by our team found that the aberrant expression of tRNA-derived small RNA (tsRNA) was involved in the development of OSF, with tiRNA-Val-CAC-002 showing the most significant difference. This study aimed to investigate the effect of tiRNA-Val-CAC-002 on fibroblast activation and its underlying mechanisms, elucidate the pathogenesis of OSF, and explore new effective targets for OSF prevention and treatment.

Methods

RT-PCR was used to detect tiRNA-Val-CAC-002 expression in OSF and arecoline-treated fibroblasts. Western blotting, MDC staining, and transmission electron microscopy validated the effects of arecoline and 002 on fibroblast autophagy. Western blotting was used to explore the signaling pathways related to tiRNA-Val-CAC-002 in OSF.

Results

Arecoline promotes fibroblast (FB) activation by upregulating tiRNA-Val-CAC-002. Arecoline stimulation and tiRNA-Val-CAC-002 overexpression activated fibroblasts by promoting autophagy. tiRNA-Val-CAC-002 regulates PI3K/AKT by mediating ITGB3 expression.

Conclusions

Arecoline upregulates tiRNA-Val-CAC-002 expression in fibroblasts. Moreover, tiRNA-Val-CAC-002 may activate the autophagy of fibroblasts in OSF by ITGB3/PI3K/AKT pathway regulation, promoting the expression of collagen fibers.

目的：口腔黏膜下纤维化（OSF）是一种慢性、进行性和潜在的口腔恶性疾病。我们团队之前的一项研究发现，tRNA衍生小RNA（tsRNA）的异常表达参与了OSF的发展，其中tiRNA-Val-CAC-002的差异最为显著。本研究旨在探讨tiRNA-Val-CAC-002对成纤维细胞活化的影响及其内在机制，阐明OSF的发病机制，并探索预防和治疗OSF的新有效靶点：方法：采用 RT-PCR 技术检测 tiRNA-Val-CAC-002 在 OSF 和异丙嗪处理的成纤维细胞中的表达。Western印迹、MDC染色和透射电子显微镜验证了arecoline和002对成纤维细胞自噬的影响。Western 印迹技术被用来探索 OSF 中与 tiRNA-Val-CAC-002 相关的信号通路：结果：Arecoline通过上调tiRNA-Val-CAC-002促进成纤维细胞（FB）的活化。tiRNA-Val-CAC-002通过介导ITGB3的表达调控PI3K/AKT：结论：Arecoline 可上调成纤维细胞中 tiRNA-Val-CAC-002 的表达。此外，tiRNA-Val-CAC-002 可通过 ITGB3/PI3K/AKT 通路调控激活 OSF 中成纤维细胞的自噬，促进胶原纤维的表达。

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引用次数: 0

Apoptotic cell death during regressive changes in salivary glands: A morphological perspective 唾液腺退行性变化过程中的细胞凋亡：形态学视角。

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-03-01 DOI: 10.1016/j.job.2024.100585

Shigeru Takahashi , Akihiro Nezu , Akihiko Tanimura , Chikage Tamura , Kenji Imamachi , Tadasu Sato

Background

Apoptosis was initially identified through transmission electron microscopy. Subsequent advances in morphological techniques for apoptosis detection have revealed its involvement in multiple pathological conditions in various tissues. This review summarizes previous experimental studies on apoptotic cell death during regressive changes in the salivary glands, with a focus on morphological observations.

Highlight

Obstructive sialadenitis is histologically characterized by acinar cell loss and increased number of duct cells. Although acinar cells were previously believed to dedifferentiate into duct cells, there is evidence that they are eliminated by apoptosis. Animals fed a soft diet exhibited parotid gland atrophy, in which acinar cells decreased in size and disappeared because of apoptosis. Age-related changes in the salivary glands involved a reduced number of acinar cell through apoptosis. Additionally, apoptotic acinar cell death occurs in other pathological conditions, including the regression of hypertrophic and irradiated salivary glands.

Conclusion

Apoptosis often eliminates acinar cells during atrophic alterations in the salivary glands. Unlike necrosis, apoptosis is an active form of cell death, thereby helping prevent the complete destruction of the salivary glands. However, the contribution of apoptosis to regressive changes in the salivary glands remains unclear and warrants further investigation.

背景：细胞凋亡最初是通过透射电子显微镜发现的。随后，用于检测细胞凋亡的形态学技术不断进步，揭示了细胞凋亡参与了各种组织的多种病理状况。本综述总结了以往关于唾液腺退行性变化过程中细胞凋亡的实验研究，重点是形态学观察：阻塞性唾液腺炎的组织学特征是尖突细胞丢失和导管细胞数量增加。尽管以前认为尖突细胞会向导管细胞分化，但有证据表明它们会因凋亡而消失。喂食软质食物的动物表现出腮腺萎缩，其中的尖突细胞因凋亡而缩小和消失。唾液腺中与年龄有关的变化包括通过细胞凋亡减少尖突细胞的数量。此外，在其他病理情况下，包括肥大的唾液腺和辐照后的唾液腺的退化，也会发生尖腺细胞凋亡：结论：在唾液腺发生萎缩性改变的过程中，凋亡往往会消灭凋亡的尖突细胞。与坏死不同，凋亡是一种主动的细胞死亡形式，因此有助于防止唾液腺的完全破坏。然而，细胞凋亡对唾液腺退行性变化的作用仍不清楚，值得进一步研究。

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引用次数: 0

Enhanced osteogenic differentiation of human periodontal ligament cells by mature osteoclasts

IF 2.6 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences

Pub Date : 2025-02-22 DOI: 10.1016/j.job.2025.100632

Sumit Suamphan , Anupong Makeudom , Suttichai Krisanaprakornkit , Pimphorn Meekhantong , Ekapong Dechtham , Chidchanok Leethanakul

Objective

Several in vitro studies have shown that reverse signaling from osteoclasts regulates osteoblast differentiation and mineralization. However, none of these studies have reported the effects of this signaling pathway on periodontal ligament (PDL) cells. Therefore, in this study, we aimed to investigate the interaction between receptor activators of nuclear factor kappa B (RANK) released from mature human osteoclasts and the membranous RANK ligand (RANKL) in human PDL cells.

Methods

Multinucleated mature human osteoclasts were differentiated from peripheral blood mononuclear cells upon incubation with recombinant macrophage colony-stimulating factor and RANKL. Mature osteoclasts and human PDL cells were characterized. A mature osteoclast-conditioned medium (OC-CM) was used to induce osteogenic differentiation of PDL cells. Mechanistic analysis of RANK-RANKL reverse signaling were conducted to determine the regulation of osteogenic induction using conditioned medium from mature osteoclasts treated with GW4869 (GW–OC–CM) or PDL cells pretreated with recombinant human osteoprotegerin (OPG).

Results

OC-CM significantly upregulated the mRNA expression of osteogenic genes and enhanced the osteogenic differentiation and biomineralization of PDL cells (p < 0.05). GW–OC–CM significantly reduced the expression of osteogenic genes, osteogenic differentiation, and biomineralization in PDL cells (p < 0.05). Similarly, the pretreatment of PDL cells with OPG before OC-CM treatment significantly reduced the osteogenic induction of PDL cells (p < 0.05).

Conclusion

Mature osteoclasts can induce osteogenesis in human PDL cells via RANK-RANKL reverse signaling.

{"title":"Enhanced osteogenic differentiation of human periodontal ligament cells by mature osteoclasts","authors":"Sumit Suamphan , Anupong Makeudom , Suttichai Krisanaprakornkit , Pimphorn Meekhantong , Ekapong Dechtham , Chidchanok Leethanakul","doi":"10.1016/j.job.2025.100632","DOIUrl":"10.1016/j.job.2025.100632","url":null,"abstract":"<div><h3>Objective</h3><div>Several <em>in vitro</em> studies have shown that reverse signaling from osteoclasts regulates osteoblast differentiation and mineralization. However, none of these studies have reported the effects of this signaling pathway on periodontal ligament (PDL) cells. Therefore, in this study, we aimed to investigate the interaction between receptor activators of nuclear factor kappa B (RANK) released from mature human osteoclasts and the membranous RANK ligand (RANKL) in human PDL cells.</div></div><div><h3>Methods</h3><div>Multinucleated mature human osteoclasts were differentiated from peripheral blood mononuclear cells upon incubation with recombinant macrophage colony-stimulating factor and RANKL. Mature osteoclasts and human PDL cells were characterized. A mature osteoclast-conditioned medium (OC-CM) was used to induce osteogenic differentiation of PDL cells. Mechanistic analysis of RANK-RANKL reverse signaling were conducted to determine the regulation of osteogenic induction using conditioned medium from mature osteoclasts treated with GW4869 (GW–OC–CM) or PDL cells pretreated with recombinant human osteoprotegerin (OPG).</div></div><div><h3>Results</h3><div>OC-CM significantly upregulated the mRNA expression of osteogenic genes and enhanced the osteogenic differentiation and biomineralization of PDL cells (<em>p</em> < 0.05). GW–OC–CM significantly reduced the expression of osteogenic genes, osteogenic differentiation, and biomineralization in PDL cells (<em>p</em> < 0.05). Similarly, the pretreatment of PDL cells with OPG before OC-CM treatment significantly reduced the osteogenic induction of PDL cells (<em>p</em> < 0.05).</div></div><div><h3>Conclusion</h3><div>Mature osteoclasts can induce osteogenesis in human PDL cells via RANK-RANKL reverse signaling.</div></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"67 2","pages":"Article 100632"},"PeriodicalIF":2.6,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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