A. Corte-Real, P. Almiro, Mafalda Silva, T. Nunes, João Abreu, Carla Carreira, Duarte Nuno Vieira
Physical violence against children and adolescents is an issue of Global Public Health. This study aims to identify traumatic injuries and the medical–legal temporary framework of the victim’s profile in the European legal approach. Participants and Setting: The clinical reports of a Portuguese European Clinical Academic Center database were analyzed. An observational and prospective cohort study was performed. A descriptive analysis of the variables was conducted, considering gender, bimodal age groups, place of residence, offender data, place of occurrence, etiology, localization, type of injuries, personal injury assessment by Quantum doloris, and injury time. The statistical analysis was performed by Spearman’s rho and Kendall’s tau-b correlation tests, Pearson’s chi-square test of independence (χ2), and Mann–Whitney and Kruskal–Wallis nonparametric tests (P < 0.05). The relationship between age groups and the place of occurrence was statistically significant (P = 0.001). Orofacial and nonorofacial injuries were related (P = 0.035). The General Data Protection Regulation is not a barrier to the treatment and sharing of justified data but a framework for safeguarding individuals’ fundamental rights, including the Right to Health. Meticulous reporting of the clinical situation involves the victim, the occurrence, and the potential offender.
{"title":"Oral health professional intervention and child physical abuse—European legal approach","authors":"A. Corte-Real, P. Almiro, Mafalda Silva, T. Nunes, João Abreu, Carla Carreira, Duarte Nuno Vieira","doi":"10.1093/fsr/owad042","DOIUrl":"https://doi.org/10.1093/fsr/owad042","url":null,"abstract":"\u0000 Physical violence against children and adolescents is an issue of Global Public Health. This study aims to identify traumatic injuries and the medical–legal temporary framework of the victim’s profile in the European legal approach. Participants and Setting: The clinical reports of a Portuguese European Clinical Academic Center database were analyzed. An observational and prospective cohort study was performed. A descriptive analysis of the variables was conducted, considering gender, bimodal age groups, place of residence, offender data, place of occurrence, etiology, localization, type of injuries, personal injury assessment by Quantum doloris, and injury time. The statistical analysis was performed by Spearman’s rho and Kendall’s tau-b correlation tests, Pearson’s chi-square test of independence (χ2), and Mann–Whitney and Kruskal–Wallis nonparametric tests (P < 0.05). The relationship between age groups and the place of occurrence was statistically significant (P = 0.001). Orofacial and nonorofacial injuries were related (P = 0.035). The General Data Protection Regulation is not a barrier to the treatment and sharing of justified data but a framework for safeguarding individuals’ fundamental rights, including the Right to Health. Meticulous reporting of the clinical situation involves the victim, the occurrence, and the potential offender.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"28 3","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138598045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A bibliometric analysis was performed to explore the current research status and development trends for missing persons globally and in East Asia and to identify research hotspots and frontiers relating to this topic. A search was conducted to identify relevant literature on missing persons using the Web of Science Core Collection database for the period 2000–2021. Subsequently, a knowledge map was constructed using CiteSpace software to perform a visual analysis of the distribution of authors and institutions, journals, national/regional distribution; citation frequency; high-frequency keywords; and emerging research hotspots. The results showed firstly that discussions on missing persons and related topics in East Asia are held at the regional scale. There is a paucity of research on this topic, which has been conducted on a limited scale, lacks depth and possibly innovation and entails limited discussion in this region. Secondly, there is a lack of social science research on missing persons and related topics worldwide. Thirdly, relevant research in East Asia should continue to preserve its own characteristics, effectively addressing current issues and enabling more people to participate in social science-oriented discussions focusing on the topic of missing persons. This approach provides a promising direction for pursuing the sustainable development of the topic of missing persons.
通过文献计量分析,探讨全球和东亚地区失踪人口研究现状和发展趋势,并确定研究热点和前沿。使用Web of Science核心收集数据库检索2000-2021年期间关于失踪人口的相关文献。随后,利用CiteSpace软件构建知识图谱,对作者分布、机构分布、期刊分布、国家/地区分布进行可视化分析;引用频率;高频关键词;以及新兴的研究热点。结果表明:东亚地区失踪人口及相关议题的讨论是在区域尺度上进行的;关于这一主题的研究很少,研究范围有限,缺乏深度和创新,在本地区的讨论也很有限。其次,全球范围内对失踪人口及相关课题的社会科学研究缺乏。第三,东亚地区的相关研究应继续保持自身特点,有效解决当前问题,使更多的人参与到以失踪人口为主题的社会科学讨论中来。这种做法为追求失踪人员专题的可持续发展提供了一个有希望的方向。
{"title":"A bibliometric analysis of research trends for missing persons globally and in East Asia from 2000 to 2021","authors":"Zixuan Zeng, Ishmael Dikeledi, Yehui Lv","doi":"10.1093/fsr/owad041","DOIUrl":"https://doi.org/10.1093/fsr/owad041","url":null,"abstract":"\u0000 A bibliometric analysis was performed to explore the current research status and development trends for missing persons globally and in East Asia and to identify research hotspots and frontiers relating to this topic. A search was conducted to identify relevant literature on missing persons using the Web of Science Core Collection database for the period 2000–2021. Subsequently, a knowledge map was constructed using CiteSpace software to perform a visual analysis of the distribution of authors and institutions, journals, national/regional distribution; citation frequency; high-frequency keywords; and emerging research hotspots. The results showed firstly that discussions on missing persons and related topics in East Asia are held at the regional scale. There is a paucity of research on this topic, which has been conducted on a limited scale, lacks depth and possibly innovation and entails limited discussion in this region. Secondly, there is a lack of social science research on missing persons and related topics worldwide. Thirdly, relevant research in East Asia should continue to preserve its own characteristics, effectively addressing current issues and enabling more people to participate in social science-oriented discussions focusing on the topic of missing persons. This approach provides a promising direction for pursuing the sustainable development of the topic of missing persons.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"21 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138596678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Massively parallel sequencing (MPS) technology has already shown many advantages in forensic DNA typing. In this study, the repeat region (RR) and flanking region (FR) variations were detected at 58 short tandem repeats (STRs) by MPS, which could increase allelic diversity (34.59%) and gene diversity (3.95%) compared with the capillary electrophoresis (CE) method. Six novel RR variants were identified at 5 STRs, and eleven FR variants were found at 8 STRs. Concordance amounted to 99.96% (4598 out of 4600 alleles) between ForenSeq DNA Signature Prep Kit and CE kits, and discordance was due to bioinformatic misinterpretation at DXS7132. Additionally, allele frequencies and forensic parameters of 94 identity-informative single nucleotide polymorphisms (iiSNPs) provided a reference dataset, which held a higher power of discrimination (1–3.4825 × 10−35). A total of 29 FR variants were found at 24 target iiSNPs. The power of the system effectiveness with the combined 27 autosomal STRs by sequence and 94 iiSNPs was added more substantially than that of a single type of marker alone, such as the combined power of discrimination (CPD) and the combined power of exclusion (CPE) achieving 1–7.1358 × 10−65 and 1–4.4803 × 10−21, respectively. Population structure analyses with iiSNPs and additional ancestry-informative SNPs (aiSNPs) demonstrated that Chinese Xibe in the northeast was one of the East Asian populations. At the genetic level, Northeastern Xibe is close to populations where they live, such as Han Chinese and other ethnic minority groups.
{"title":"Sequence variations of 58 STRs and 94 SNPs in Northeastern Xibe with ForenSeq™ DNA signature prep kit","authors":"Fei Guo, Longnian Zhang, Y. Xin, Shiquan Liu","doi":"10.1093/fsr/owad043","DOIUrl":"https://doi.org/10.1093/fsr/owad043","url":null,"abstract":"\u0000 Massively parallel sequencing (MPS) technology has already shown many advantages in forensic DNA typing. In this study, the repeat region (RR) and flanking region (FR) variations were detected at 58 short tandem repeats (STRs) by MPS, which could increase allelic diversity (34.59%) and gene diversity (3.95%) compared with the capillary electrophoresis (CE) method. Six novel RR variants were identified at 5 STRs, and eleven FR variants were found at 8 STRs. Concordance amounted to 99.96% (4598 out of 4600 alleles) between ForenSeq DNA Signature Prep Kit and CE kits, and discordance was due to bioinformatic misinterpretation at DXS7132. Additionally, allele frequencies and forensic parameters of 94 identity-informative single nucleotide polymorphisms (iiSNPs) provided a reference dataset, which held a higher power of discrimination (1–3.4825 × 10−35). A total of 29 FR variants were found at 24 target iiSNPs. The power of the system effectiveness with the combined 27 autosomal STRs by sequence and 94 iiSNPs was added more substantially than that of a single type of marker alone, such as the combined power of discrimination (CPD) and the combined power of exclusion (CPE) achieving 1–7.1358 × 10−65 and 1–4.4803 × 10−21, respectively. Population structure analyses with iiSNPs and additional ancestry-informative SNPs (aiSNPs) demonstrated that Chinese Xibe in the northeast was one of the East Asian populations. At the genetic level, Northeastern Xibe is close to populations where they live, such as Han Chinese and other ethnic minority groups.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"24 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138596610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-09DOI: 10.3390/forensicsci3040041
Alexander F. Christensen
This communication reports the relationship between skeletal age estimates based on the sternal rib ends (using the phase method of İşcan and colleagues, later revised by Hartnett) and the chronological age at the death of 221 U.S. military casualties processed by the Scientific Analysis section of the Defense POW/MIA Accounting Agency and its predecessor laboratories from 2000 to the present. Previously published age ranges for each phase do not provide accurate estimates for a sufficient proportion of the cases for forensic use; as an example, the age ranges that accompany reference cast sets proved accurate for 55% of CIL cases. Combining the published age ranges of İşcan and Hartnett, on the other hand, proved accurate in 99% of the cases.
{"title":"Sternal Rib Ends as a Method of Age Estimation at the CIL: A Brief Note","authors":"Alexander F. Christensen","doi":"10.3390/forensicsci3040041","DOIUrl":"https://doi.org/10.3390/forensicsci3040041","url":null,"abstract":"This communication reports the relationship between skeletal age estimates based on the sternal rib ends (using the phase method of İşcan and colleagues, later revised by Hartnett) and the chronological age at the death of 221 U.S. military casualties processed by the Scientific Analysis section of the Defense POW/MIA Accounting Agency and its predecessor laboratories from 2000 to the present. Previously published age ranges for each phase do not provide accurate estimates for a sufficient proportion of the cases for forensic use; as an example, the age ranges that accompany reference cast sets proved accurate for 55% of CIL cases. Combining the published age ranges of İşcan and Hartnett, on the other hand, proved accurate in 99% of the cases.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":" 39","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135241747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-07DOI: 10.3390/forensicsci3040040
Olen R. Brown
Motor vehicle accidents are a significant consequence of marijuana abuse. Limitations of its roadside detection and the forensic problems with establishing impairment require innovations that are scientifically achievable. Marijuana abuse currently is at an all-time high in the United States and its physiological effects make it a popular recreational drug that is reported to be a leading cause of preventable morbidity and mortality among the youth in the 18 most affluent nations. The medical benefits of drugs derived from marijuana complicate its forensic regulation. In an extensive 2017 report by The American Academy of Sciences, the evidence for the medical benefits of delta-9 tetrahydrocannabinol (derived from marijuana) were stated to be conclusive or substantial for the treatment of chronic pain in adults, as an antiemetic for treatment of chemotherapy-induced nausea and vomiting, and for improving patient-reported multiple sclerosis spasticity symptoms. The benefits from treatment for many other medical conditions were inconclusive or absent. The evidence is clear that safely driving a motor vehicle is significantly impaired while under the influence of marijuana. However, there is no roadside forensic test that reliably detects impairment, and there is an urgent need for such to protect the public while insuring the legal rights of users. There is strong societal pressure to relax marijuana’s forensic regulation, including removing it from Schedule I of the Controlled Substances Act. Artificial intelligence (AI) should be implemented with appropriate human control to resolve controversies.
{"title":"Marijuana: Forensics of Abuse, Medical Uses, Controversy, and AI","authors":"Olen R. Brown","doi":"10.3390/forensicsci3040040","DOIUrl":"https://doi.org/10.3390/forensicsci3040040","url":null,"abstract":"Motor vehicle accidents are a significant consequence of marijuana abuse. Limitations of its roadside detection and the forensic problems with establishing impairment require innovations that are scientifically achievable. Marijuana abuse currently is at an all-time high in the United States and its physiological effects make it a popular recreational drug that is reported to be a leading cause of preventable morbidity and mortality among the youth in the 18 most affluent nations. The medical benefits of drugs derived from marijuana complicate its forensic regulation. In an extensive 2017 report by The American Academy of Sciences, the evidence for the medical benefits of delta-9 tetrahydrocannabinol (derived from marijuana) were stated to be conclusive or substantial for the treatment of chronic pain in adults, as an antiemetic for treatment of chemotherapy-induced nausea and vomiting, and for improving patient-reported multiple sclerosis spasticity symptoms. The benefits from treatment for many other medical conditions were inconclusive or absent. The evidence is clear that safely driving a motor vehicle is significantly impaired while under the influence of marijuana. However, there is no roadside forensic test that reliably detects impairment, and there is an urgent need for such to protect the public while insuring the legal rights of users. There is strong societal pressure to relax marijuana’s forensic regulation, including removing it from Schedule I of the Controlled Substances Act. Artificial intelligence (AI) should be implemented with appropriate human control to resolve controversies.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"106 S7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135540384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-02DOI: 10.3390/forensicsci3040039
Edda E. Guareschi, Sara Poggesi, Marco Palmesino, Paola A. Magni
Introduction: Three tiny bones compose the human ossicular chain: malleus, incus and stapes. Also known as auditory ossicles, they are united by joints in the middle ear cavity of the petrous part of the temporal bone. Completely developed two years after birth, the ossicular chain is involved in the physiological process of hearing, by which sound waves from the environment are converted into electrochemical impulses. In the last 500 years, most studies have focused on the morphogenesis, morphological variability and clinical pathology of the ossicular chain, whilst only a few studies have added relevant knowledge to anthropology and forensic science. The auditory ossicles and the enclosing petrous bone are some of the hardest in the human skeleton. This is reflected in a relative resistance to fire and in the possibility of preservation and fossilization in millions of years. Materials and Methods: The literature and four present-day forensic cases were included in studying the postmortem loss of the auditory ossicles in skeletal or decomposing remains. Results indicate that it can be ascribed to their destruction or physical displacement, by either macro-micro-faunal action and/or any other natural or artificial disturbance. Discussion: Physical displacement is closely connected to the depositional environment of the skeletal remains, such as burial, entombment (sarcophagus, coffin, vault…), submersion or exposure to natural elements. Auditory ossicles can be recovered in situ, or very close to their anatomical location, when the skeletal material has been involved in an archaeological excavation. In the case of accessible or disturbed remains, scavengers may remove the tiny ossicles and/or they can slip out of the middle ear cavity following skull movements. Entombment offers effective protection against the displacement of the auditory ossicles, whereas aquatic submersion and aquatic movement almost invariably displace them. Conclusion: the preservation of the human auditory ossicles should be critically considered in the comprehensive context of any forensic investigation on human remains since it can assist the reconstruction of their taphonomic history. Taphonomic histories of remains can add crucial information to forensic investigations (e.g., the Post Mortem Interval, PMI). The aim of this study, limited by scarce relevant literature, is to discuss the potential role of the ossicular chain, detected by postmortem imaging techniques, as a taphonomical indicator in decomposing and/or skeletonized bodies.
{"title":"The Presence of the Human Auditory Ossicles—Detected Postmortem by CT Scan—As a Taphonomic Indicator","authors":"Edda E. Guareschi, Sara Poggesi, Marco Palmesino, Paola A. Magni","doi":"10.3390/forensicsci3040039","DOIUrl":"https://doi.org/10.3390/forensicsci3040039","url":null,"abstract":"Introduction: Three tiny bones compose the human ossicular chain: malleus, incus and stapes. Also known as auditory ossicles, they are united by joints in the middle ear cavity of the petrous part of the temporal bone. Completely developed two years after birth, the ossicular chain is involved in the physiological process of hearing, by which sound waves from the environment are converted into electrochemical impulses. In the last 500 years, most studies have focused on the morphogenesis, morphological variability and clinical pathology of the ossicular chain, whilst only a few studies have added relevant knowledge to anthropology and forensic science. The auditory ossicles and the enclosing petrous bone are some of the hardest in the human skeleton. This is reflected in a relative resistance to fire and in the possibility of preservation and fossilization in millions of years. Materials and Methods: The literature and four present-day forensic cases were included in studying the postmortem loss of the auditory ossicles in skeletal or decomposing remains. Results indicate that it can be ascribed to their destruction or physical displacement, by either macro-micro-faunal action and/or any other natural or artificial disturbance. Discussion: Physical displacement is closely connected to the depositional environment of the skeletal remains, such as burial, entombment (sarcophagus, coffin, vault…), submersion or exposure to natural elements. Auditory ossicles can be recovered in situ, or very close to their anatomical location, when the skeletal material has been involved in an archaeological excavation. In the case of accessible or disturbed remains, scavengers may remove the tiny ossicles and/or they can slip out of the middle ear cavity following skull movements. Entombment offers effective protection against the displacement of the auditory ossicles, whereas aquatic submersion and aquatic movement almost invariably displace them. Conclusion: the preservation of the human auditory ossicles should be critically considered in the comprehensive context of any forensic investigation on human remains since it can assist the reconstruction of their taphonomic history. Taphonomic histories of remains can add crucial information to forensic investigations (e.g., the Post Mortem Interval, PMI). The aim of this study, limited by scarce relevant literature, is to discuss the potential role of the ossicular chain, detected by postmortem imaging techniques, as a taphonomical indicator in decomposing and/or skeletonized bodies.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"61 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135933527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract ChatGPT is a variant of the GPT (Generative Pre-trained Transformer) language model that uses large amounts of text-based training data and a transformer architecture to generate human-like text adjusted to the received prompts. ChatGPT presents several advantages in forensic sciences, namely constituting a virtual assistant to aid lawyers, judges, and victims in managing and interpreting forensic expert data. But what would happen if ChatGPT began to be used to produce forensic expertise reports? Despite its potential applications, the use of ChatGPT and other Large Language Models (LLM) and artificial intelligence (AI) tools in forensic writing also poses ethical and legal concerns, which are discussed in this perspective together with some expected future perspectives.
{"title":"ChatGPT in forensic sciences: a new Pandora’s box with advantages and challenges to pay attention","authors":"Ricardo Jorge Dinis-Oliveira, Rui M S Azevedo","doi":"10.1093/fsr/owad039","DOIUrl":"https://doi.org/10.1093/fsr/owad039","url":null,"abstract":"Abstract ChatGPT is a variant of the GPT (Generative Pre-trained Transformer) language model that uses large amounts of text-based training data and a transformer architecture to generate human-like text adjusted to the received prompts. ChatGPT presents several advantages in forensic sciences, namely constituting a virtual assistant to aid lawyers, judges, and victims in managing and interpreting forensic expert data. But what would happen if ChatGPT began to be used to produce forensic expertise reports? Despite its potential applications, the use of ChatGPT and other Large Language Models (LLM) and artificial intelligence (AI) tools in forensic writing also poses ethical and legal concerns, which are discussed in this perspective together with some expected future perspectives.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"2002 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135412967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-17DOI: 10.3390/forensicsci3040038
Juliana Curtis, Landon Stitle, Jessica Certain, Madeline Murchland, Charlotte Piszel, Jordan Vest, Claire L. McLeod, Mark P. S. Krekeler
Interpersonal violence is a rising issue in global society and new approaches are being sought to combat the problem. Within this context, expanding forensic techniques to better document violent crime scenes is critical for improving and acquiring legal evidence, such as proving or tracing contact between victims and suspects. This project aims to demonstrate the potential for forensic investigations in the context of interpersonal violence using a field-based reflective spectroscopy approach. For this, a common cosmetic, Wet‘N’Wild “Color Icon” blush in the shade “Pearlescent Pink”, was mineralogically characterized using transmission electron microscopy and powder X-ray diffraction and subsequently investigated via reflective spectroscopy on a variety of common substrates. Differing amounts of the cosmetic product, ranging from 0.001 g to 0.075 g, were applied to a variety of substrates using a simple push method to simulate forcible contact and material transfer. Substrates included a pine wood block; (calcareous) sand from Tulum, Mexico; Ottawa sand; tile; Pergo wood; linoleum; closet material; carpets; and fabrics. The reflective spectra of cosmetic–substrate combinations were measured via an ASD FieldSpec 4 Hi-Res spectroradiometer. The Wet‘N’Wild cosmetic was reliably detected on various substrates relevant to crime scenes. Minor amounts (as low as 0.02 mg/mm2) could be detected, and average limits of detection of 0.03 mg/mm2) were achieved; however, a calcareous sand (Tulum) had a high level of detection (>0.38 mg/mm2), suggesting that further investigation is needed for more complex sand substrates. The use of the ASD spectroradiometer as a forensic tool within the context of crime scene documentation shows promise. Future work should expand the characterization of cosmetic materials across a broad range of substrates and consider systematic studies of specific population groups. Furthermore, combining this approach with hyperspectral imaging at crime scenes is a promising future direction for crime scene documentation. This work therefore demonstrates a novel method for investigating cosmetics within the context of interpersonal violence and provides a foundation for future laboratory and field work using the ASD FieldSpec 4 and hyperspectral imaging systems.
人际暴力是全球社会中一个日益严重的问题,人们正在寻求新的方法来解决这个问题。在这种情况下,扩大法医技术以更好地记录暴力犯罪现场对于改进和获取法律证据至关重要,例如证明或追踪受害者与嫌疑人之间的接触。该项目旨在展示利用基于现场的反射光谱方法在人际暴力背景下进行法医调查的潜力。为此,一种常见的化妆品,Wet 'N 'Wild“Color Icon”腮红,在“珠光粉色”阴影下,使用透射电子显微镜和粉末x射线衍射进行矿物学表征,随后通过反射光谱在各种常见基材上进行研究。使用简单的推法将不同量的化妆品(从0.001 g到0.075 g)应用于各种基材上,以模拟强制接触和材料转移。基材包括松木块;(钙质)砂来自墨西哥图卢姆;渥太华砂;瓷砖;Pergo木材;油毡;衣柜材料;地毯;和面料。通过ASD fieldspec4高分辨率光谱辐射计测量了化妆品-衬底组合的反射光谱。在与犯罪现场相关的各种基材上可靠地检测到Wet 'N 'Wild化妆品。可以检测到少量(低至0.02 mg/mm2),平均检测限为0.03 mg/mm2);然而,钙质砂(Tulum)的检测水平很高(>0.38 mg/mm2),这表明需要对更复杂的砂基质进行进一步研究。在犯罪现场记录的背景下,使用ASD光谱辐射计作为法医工具显示了前景。未来的工作应该扩大化妆品材料在广泛的底物范围内的表征,并考虑对特定人群的系统研究。此外,将这种方法与犯罪现场的高光谱成像相结合,是犯罪现场记录的一个有希望的未来方向。因此,这项工作展示了一种在人际暴力背景下研究化妆品的新方法,并为未来使用ASD fieldspec4和高光谱成像系统的实验室和现场工作奠定了基础。
{"title":"A Reflective Spectroscopy and Mineralogical Investigation of Cosmetic Blush (Wet‘N’Wild) Potentially for Forensic Investigations Related to Interpersonal Violence—An Experimental Feasibility Study","authors":"Juliana Curtis, Landon Stitle, Jessica Certain, Madeline Murchland, Charlotte Piszel, Jordan Vest, Claire L. McLeod, Mark P. S. Krekeler","doi":"10.3390/forensicsci3040038","DOIUrl":"https://doi.org/10.3390/forensicsci3040038","url":null,"abstract":"Interpersonal violence is a rising issue in global society and new approaches are being sought to combat the problem. Within this context, expanding forensic techniques to better document violent crime scenes is critical for improving and acquiring legal evidence, such as proving or tracing contact between victims and suspects. This project aims to demonstrate the potential for forensic investigations in the context of interpersonal violence using a field-based reflective spectroscopy approach. For this, a common cosmetic, Wet‘N’Wild “Color Icon” blush in the shade “Pearlescent Pink”, was mineralogically characterized using transmission electron microscopy and powder X-ray diffraction and subsequently investigated via reflective spectroscopy on a variety of common substrates. Differing amounts of the cosmetic product, ranging from 0.001 g to 0.075 g, were applied to a variety of substrates using a simple push method to simulate forcible contact and material transfer. Substrates included a pine wood block; (calcareous) sand from Tulum, Mexico; Ottawa sand; tile; Pergo wood; linoleum; closet material; carpets; and fabrics. The reflective spectra of cosmetic–substrate combinations were measured via an ASD FieldSpec 4 Hi-Res spectroradiometer. The Wet‘N’Wild cosmetic was reliably detected on various substrates relevant to crime scenes. Minor amounts (as low as 0.02 mg/mm2) could be detected, and average limits of detection of 0.03 mg/mm2) were achieved; however, a calcareous sand (Tulum) had a high level of detection (>0.38 mg/mm2), suggesting that further investigation is needed for more complex sand substrates. The use of the ASD spectroradiometer as a forensic tool within the context of crime scene documentation shows promise. Future work should expand the characterization of cosmetic materials across a broad range of substrates and consider systematic studies of specific population groups. Furthermore, combining this approach with hyperspectral imaging at crime scenes is a promising future direction for crime scene documentation. This work therefore demonstrates a novel method for investigating cosmetics within the context of interpersonal violence and provides a foundation for future laboratory and field work using the ASD FieldSpec 4 and hyperspectral imaging systems.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136032969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract MicroRNAs (miRNAs) are a class of small non-coding RNAs that exert their biological functions as negative regulators of gene expression. They are involved in the skin wound healing process with a dynamic expression pattern and can therefore potentially serve as biomarkers for skin wound age estimation. However, no reports have described any miRNAs as suitable reference genes (RGs) for miRNA quantification in wounded skin or samples with postmortem changes. Here, we aimed to identify specific miRNAs as RGs for miRNA quantification to support further studies of skin wound age estimation. Overall, nine miRNAs stably expressed in mouse skin at certain posttraumatic intervals (PTIs) were preselected by next-generation sequencing as candidate RGs. These nine miRNAs and the commonly used reference genes (comRGs: U6, GAPDH, ACTB,18S, 5S, LC-Ogdh) were quantitatively examined using qRT-PCR at different PTIs during skin wound healing in mice. The stabilities of these genes were evaluated using four independent algorithms: GeNorm, NormFinder, Bestkeeper, and comparative Delta Ct. Stability was further evaluated in mice with different postmortem intervals (PMIs). Overall, mmu-miR-26a-5p, mmu-miR-30d-5p, and mmu-miR-152-3p were identified as the most stable genes at both different PTIs and PMIs. These three miRNA RGs were additionally validated and compared with the comRGs in human samples. After assessing using one, two, or three miRNAs in combination for stability at different PTIs, PMIs, or in human samples, the set of miR-26a/30d/152 was approved as the best normalizer. In conclusion, our data suggest that the combination of miR-26a/30d/152 is recommended as the normalization strategy for miRNA qRT-PCR quantification in skin wound age estimation.
{"title":"miR-26a/30d/152 are reliable reference genes for miRNA quantification in skin wound age estimation","authors":"Longlong Suo, Jian Cheng, Haomiao Yuan, Zhenf Fei Jiang, Dilichati Tash, Linlin Wang, Hao Cheng, Zhongduo Zhang, Fuyuan Zhang, Miao Zhang, Zhipeng Cao, Rui Zhao, Dawei Guan","doi":"10.1093/fsr/owad037","DOIUrl":"https://doi.org/10.1093/fsr/owad037","url":null,"abstract":"Abstract MicroRNAs (miRNAs) are a class of small non-coding RNAs that exert their biological functions as negative regulators of gene expression. They are involved in the skin wound healing process with a dynamic expression pattern and can therefore potentially serve as biomarkers for skin wound age estimation. However, no reports have described any miRNAs as suitable reference genes (RGs) for miRNA quantification in wounded skin or samples with postmortem changes. Here, we aimed to identify specific miRNAs as RGs for miRNA quantification to support further studies of skin wound age estimation. Overall, nine miRNAs stably expressed in mouse skin at certain posttraumatic intervals (PTIs) were preselected by next-generation sequencing as candidate RGs. These nine miRNAs and the commonly used reference genes (comRGs: U6, GAPDH, ACTB,18S, 5S, LC-Ogdh) were quantitatively examined using qRT-PCR at different PTIs during skin wound healing in mice. The stabilities of these genes were evaluated using four independent algorithms: GeNorm, NormFinder, Bestkeeper, and comparative Delta Ct. Stability was further evaluated in mice with different postmortem intervals (PMIs). Overall, mmu-miR-26a-5p, mmu-miR-30d-5p, and mmu-miR-152-3p were identified as the most stable genes at both different PTIs and PMIs. These three miRNA RGs were additionally validated and compared with the comRGs in human samples. After assessing using one, two, or three miRNAs in combination for stability at different PTIs, PMIs, or in human samples, the set of miR-26a/30d/152 was approved as the best normalizer. In conclusion, our data suggest that the combination of miR-26a/30d/152 is recommended as the normalization strategy for miRNA qRT-PCR quantification in skin wound age estimation.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135759330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-10DOI: 10.3390/forensicsci3040037
Pier Matteo Barone, Rosa Maria Di Maggio
Forensic geoarchaeology provides a comprehensive and interdisciplinary approach to investigating crime scenes. This field combines the techniques and methods of archaeology, geology, geophysics, and remote sensing, to provide a more comprehensive picture of the events that have occurred at a site. Forensic geoarchaeologists can not only provide valuable information about the context of a crime scene but also help reconstruct past events and collect relevant information about missing persons. Moreover, the use of geospatial technologies and the analysis of evidence together with a vast range of scientific methods and techniques help forensic geoarchaeologists provide compelling and reliable evidence before courts and law enforcement agencies. Although some reticence still prevails among Italians about recognizing forensic geoarchaeology as a fundamental discipline vis-a-vis crime scenes, the recognition of its vital significance is growing in Italy, thanks to the decision of the Italian Standards Organization (UNI) to standardize it alongside other fields of criminalistic expertise.
{"title":"Exploring the Growing Importance of Forensic Geoarchaeology in Italy","authors":"Pier Matteo Barone, Rosa Maria Di Maggio","doi":"10.3390/forensicsci3040037","DOIUrl":"https://doi.org/10.3390/forensicsci3040037","url":null,"abstract":"Forensic geoarchaeology provides a comprehensive and interdisciplinary approach to investigating crime scenes. This field combines the techniques and methods of archaeology, geology, geophysics, and remote sensing, to provide a more comprehensive picture of the events that have occurred at a site. Forensic geoarchaeologists can not only provide valuable information about the context of a crime scene but also help reconstruct past events and collect relevant information about missing persons. Moreover, the use of geospatial technologies and the analysis of evidence together with a vast range of scientific methods and techniques help forensic geoarchaeologists provide compelling and reliable evidence before courts and law enforcement agencies. Although some reticence still prevails among Italians about recognizing forensic geoarchaeology as a fundamental discipline vis-a-vis crime scenes, the recognition of its vital significance is growing in Italy, thanks to the decision of the Italian Standards Organization (UNI) to standardize it alongside other fields of criminalistic expertise.","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136358504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}