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Quantifying forensically relevant vertebrate scavenging: a pilot study to develop a methodological framework using Cape grey mongoose (Galerella pulverulenta) as an illustrative model. 量化与法医相关的脊椎动物食腐:一项以角灰猫鼬(Galerella粉鼬)为说明性模型开发方法学框架的试点研究。
IF 1.4 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-12-03 eCollection Date: 2025-06-01 DOI: 10.1093/fsr/owae069
Devin Alexander Finaughty, Gabriella May French, Kara Sierra Adams, Maximilian Jan Spies, Victoria Elaine Gibbon

Currently, forensic death investigations in the Western Cape, South Africa do not account for vertebrate scavenging activity; however, previous research in the city of Cape Town has shown a significant impact on the rate of decomposition due to scavenging by the local Cape grey mongoose (Galerella pulverulenta). This pilot study aimed to develop a framework to more robustly quantifiably describe and analyse the scavenging behaviour of this species on a single clothed 60 kg porcine carcass in a forensically significant location in Cape Town (i.e., a region of the city with a proportionately large forensic case load). Feeding behaviours are the focus of this framework and analysis, but non-feeding behaviours in the immediate vicinity of the carcass are also distinguished and described. Additionally, it was assessed whether all behaviours varied in their frequency of occurrence as decomposition progressed. More than 40 h of mongoose interaction with the carcass were recorded using motion-activated infrared-capable wildlife trail camera videography. The highest frequencies of scavenging activity were observed in the abdominal and the eye socket regions of the carcass. Abdominal feeding largely involved soft tissue modification, whereas more intensive activity in the eye socket suggested a higher likelihood of hard tissue scavenger artefacts being found there. A Kruskal-Wallis test confirmed that some feeding behaviours occur more frequently during specific decomposition stages. For example, scratching is common during the earlier stages of decomposition, ostensibly to create an opening in the flesh. Twisting whilst biting was only observed in latter stages of decomposition, likely due to the increased toughness of the soft tissue as it desiccated. This pilot research offers detailed insight into scavenger behaviours previously unreported in the forensic taphonomic literature, and proposes a provisional method of quantifiable analyses of scavenger behaviours that extends what has previously been published in the forensic taphonomic literature. Local validation of the observations is planned, and international replication of the research for diverse scavenger guilds is encouraged.

Key points: Novel framework for detailed quantitative analysis of vertebrate scavenger behavior using the Cape grey mongoose as a model.First systematic study of scavenger behaviour in South Africa and likely Africa-wide.Cape grey mongoose preying on blow fly larvae, emphasizing the necessity of videographic documentation.

目前,南非西开普省的法医死亡调查没有考虑到脊椎动物的食腐活动;然而,先前在开普敦市进行的研究表明,由于当地开普灰猫鼬(Galerella powder ulenta)的食腐,对分解速度产生了重大影响。这项试点研究旨在建立一个框架,以更可靠地定量描述和分析该物种在开普敦一个法医重要地点(即,该市一个法医案件数量相对较大的地区)对一具60公斤的单衣猪尸体的食腐行为。摄食行为是这个框架和分析的重点,但在尸体附近的非摄食行为也被区分和描述。此外,还评估了随着分解的进展,所有行为的发生频率是否发生了变化。使用运动激活红外野生动物跟踪摄像机记录了猫鼬与尸体之间超过40小时的相互作用。在胴体的腹部和眼窝区域观察到最高频率的清除活动。腹部喂养主要涉及软组织改造,而在眼窝中更密集的活动表明在那里发现硬组织清除物的可能性更高。Kruskal-Wallis试验证实,某些摄食行为在特定的分解阶段发生得更频繁。例如,在腐烂的早期阶段,抓挠很常见,表面上是为了在肉上开一个口。扭曲而咬只在分解的后期阶段观察到,可能是由于软组织的韧性增加,因为它干燥。这项试点研究提供了对以前在法医藏语文献中未报道的清食动物行为的详细见解,并提出了一种可量化分析清食动物行为的临时方法,该方法扩展了以前在法医藏语文献中发表的内容。计划对观察结果进行当地验证,并鼓励在不同的清道夫行会进行国际复制研究。重点:以角灰猫鼬为模型,详细定量分析脊椎动物食腐动物行为的新框架。首次对南非乃至整个非洲的食腐动物行为进行系统研究。角灰猫鼬捕食苍蝇幼虫,强调录像记录的必要性。
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引用次数: 0
Impact of liquid antibacterial soap and hand sanitizer on DNA transfer in forensic investigations: an experimental study. 液体抗菌皂和洗手液对法医调查中DNA转移的影响:一项实验研究。
IF 1.4 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-12-03 eCollection Date: 2025-06-01 DOI: 10.1093/fsr/owae068
Francesco Sessa, Elisabetta Panepinto, Monica Salerno, Mario Chisari, Massimiliano Esposito, Giuseppe Cocimano, Cristoforo Pomara

DNA transfer, whether intentional or not, is becoming an important part of forensic evidence gathering and analysis. This study seeks to determine the impact of liquid antibacterial soap and hand sanitizer use on the DNA present in the palms of hands that may potentially reduce direct transfer. Twelve volunteers were enrolled and typed. Afterwards, each palm was swabbed without considering previous activities to collect background DNA profiles. Subsequently, each subject washed his/her hands for 30 s with antibacterial soap first, and after with sanitizing gel and then air-dried them; after drying, each palm was immediately swabbed for DNA typing. The results of this study demonstrated that the possibility of recovering a complete profile from a hand swab is strictly related to previous activity: considering the results of the samples taken before handwashing, there is the possibility of having a median recovery of 80.01%, while it is very low (median recovery was 3.43%) after hand washing with antibacterial soap followed by alcohol-based hand sanitizer. Analyzing the results after handwashing, we were unable to detect any drop-in alleles. Moreover, we determined that in 11/12 samples, less than nine alleles were detected: considering that the kit used for the profiling could analyze 23 short tandem repeats (STRs), it is possible to conclude that we obtained inconclusive profiles. Based on the findings of the present study, it is more difficult to obtain a perpetrator's profile if he/she used liquid antibacterial soap and hand sanitizer immediately before the criminal action.

DNA转移,无论是有意还是无意,正成为法医证据收集和分析的重要组成部分。这项研究旨在确定使用液体抗菌肥皂和洗手液对手掌中存在的DNA的影响,这可能会减少直接转移。12名志愿者被登记并打字。之后,在不考虑之前收集背景DNA档案的情况下,每个手掌都被擦拭。随后,每位受试者先用抗菌肥皂洗手30秒,再用消毒凝胶洗手,然后风干;干燥后,立即用棉签擦拭每只手掌进行DNA分型。本研究的结果表明,从手签中恢复完整的个人资料的可能性与之前的活动密切相关:考虑到洗手前采集的样本结果,有可能有80.01%的中位数回收率,而用抗菌肥皂洗手后,酒精类洗手液洗手的中位数回收率非常低(3.43%)。在洗手后分析结果,我们无法检测到任何插入等位基因。此外,我们确定在11/12个样本中检测到的等位基因少于9个:考虑到用于分析的试剂盒可以分析23个短串联重复序列(STRs),我们可能得出结论,我们获得了不确定的基因图谱。根据本研究的结果,如果犯罪者在犯罪行为之前立即使用了液体抗菌肥皂和洗手液,则更难获得犯罪者的侧写。
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引用次数: 0
Dynamic signatures: a mathematical approach to analysis. 动态签名:一种数学分析方法。
IF 1.4 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-11-05 eCollection Date: 2024-12-01 DOI: 10.1093/fsr/owae067
Jessica Baleiro Okado, Erick Simões da Camara E Silva, Priscila Dias Sily

This study evaluates mathematical tools (principal component analysis, dynamic time warping, and the Kolmogorov-Smirnov hypothesis test) to analyse global and local data from dynamic signatures to reduce subjectivity and increase the reproducibility of handwriting examination using a two-step approach. A dataset composed of 1 800 genuine signature samples, 870 simulated signatures, and 60 disguises (30 formally similar or "autosimulated" and 30 random but different from usual) provided by 30 volunteers was collected. The first step involved global data analysis using principal component analysis and a hypothesis test performed for 62 global characteristics, and associations of these characteristics were analysed through calculations of multivariate distance followed by a hypothesis test. The second step involved the analysis of local characteristics including vertical and horizontal positions, speed, pressure gradient, acceleration, and jerk point-to-point, by using dynamic time warping followed by a hypothesis test. Optimization of sensitivity and specificity metrics of the hypothesis test was explored by varying its stringency and observing accuracy rates for the simulated and genuine groups. A P-value threshold of 1 × 10-10 was found to be optimal, making the test more restrictive and yielding accuracy rates of 96.7% for genuine global data and 88.9% for simulated data. The same cut-off value for local characteristics provided an average accuracy rate of 95.4% for genuine samples and 94.7% for simulated samples, demonstrating high accuracy for both simulated and genuine samples. However, the method did not offer reasonable accuracy rates for disguises, consistent with observations in traditional handwriting examination. Our approach provided satisfactory results for forensic examination use. The visualization of graphs and signatures and analysis of all identifying elements of handwriting by the examining expert are still essential. In future studies, we plan to perform blind tests to validate our approach and propose a rigorous methodology.

本研究评估了数学工具(主成分分析、动态时间扭曲和Kolmogorov-Smirnov假设检验)来分析动态签名的全局和局部数据,以减少主观性,并使用两步法提高笔迹检查的可重复性。收集了由30名志愿者提供的1800个真实签名样本、870个模拟签名和60个伪装签名(30个形式相似或“自动模拟”,30个随机但不同于平常)组成的数据集。第一步涉及使用主成分分析进行全球数据分析,并对62个全球特征进行假设检验,并通过计算多变量距离来分析这些特征之间的关联,然后进行假设检验。第二步是分析局部特征,包括垂直和水平位置、速度、压力梯度、加速度和点对点的震动,方法是使用动态时间翘曲,然后进行假设检验。通过改变假设检验的严格程度和观察模拟组和真实组的准确率,探索了假设检验的敏感性和特异性指标的优化。发现p值阈值为1 × 10-10是最优的,使测试更具限制性,对真实全球数据的准确率为96.7%,对模拟数据的准确率为88.9%。对于相同的局部特征截断值,正品样品的平均准确率为95.4%,模拟样品的平均准确率为94.7%,表明模拟样品和正品样品的准确率都很高。然而,该方法并没有提供合理的准确率伪装,在传统的笔迹检查观察一致。我们的方法为法医鉴定提供了满意的结果。图形和签名的可视化以及检查专家对笔迹的所有识别元素的分析仍然是必不可少的。在未来的研究中,我们计划进行盲测来验证我们的方法,并提出一个严格的方法。
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引用次数: 0
An experimental study on distinguishing gel pen ink stains using desorption electrospray ionization mass spectroscopy combined with the K-means algorithm. 解吸电喷雾电离质谱结合K-means算法鉴别凝胶笔墨迹的实验研究。
IF 1.4 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-10-22 eCollection Date: 2024-12-01 DOI: 10.1093/fsr/owae066
Yiting Yuan, Yu Tao, Da Qin

In the realm of document examination, the identification of suspicious alterations to handwritten documents is an important factor in case characterization. Investigating the differences in gel pen ink compositions has significant implications. In this study, we used desorption electrospray ionization mass spectrometry (DESI-MS) to analyze the ink compositions of gel pens. The methodology involved the following steps. (i) Sample selection: a total of 227 gel pens available in the market were procured for the study. (ii) Pre-experimental parameter exploration: preliminary experiments were performed to optimize the experimental parameters. (iii) Analytical technique: DESI-MS was used to collect compositional data from the gel pen ink samples, without requiring pre-treatment of the samples. (iv) Data analysis: the obtained data were analyzed using the Davies-Bouldin index, Calinski-Harabasz index, and K-means algorithm for ink sample classification. The experimental findings indicated that DESI-MS is a viable method for examining the ink compositions of gel pens. Notably, the testing process is minimally destructive and does not necessitate pre-treatment of the samples. Furthermore, variations in the ink compositions were observed among different models of gel pens within the same brand, and the extent of the variation in the composition varied across brands. Additionally, there were instances in which the ink compositions of different brands of gel pens exhibited similarities.

在文件检查领域,对手写文件的可疑更改的识别是案件定性的重要因素。研究凝胶笔墨水成分的差异具有重要意义。本研究采用解吸式电喷雾质谱法(DESI-MS)分析了凝胶笔的油墨成分。该方法包括以下步骤。(i)样本选择:本研究共采购了227支市面上可买到的凝胶笔。(ii)实验前参数探索:进行初步实验,优化实验参数。(iii)分析技术:使用DESI-MS收集凝胶笔墨水样品的成分数据,无需对样品进行预处理。(iv)数据分析:使用Davies-Bouldin指数、Calinski-Harabasz指数和K-means算法对得到的数据进行分析,进行油墨样本分类。实验结果表明,DESI-MS是一种可行的凝胶笔墨水成分检测方法。值得注意的是,测试过程的破坏性最小,不需要对样品进行预处理。此外,在同一品牌的不同型号的凝胶笔中观察到墨水成分的变化,并且在不同品牌的成分变化的程度不同。此外,不同品牌的凝胶笔的墨水成分也有相似之处。
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引用次数: 0
Correction to: Metric analysis of the patella for sex estimation in a Portuguese sample. 更正:对葡萄牙样本中用于性别估计的髌骨进行度量分析。
IF 1.4 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-10-17 eCollection Date: 2024-09-01 DOI: 10.1093/fsr/owae056

[This corrects the article DOI: 10.1093/fsr/owae015.].

[此处更正了文章 DOI:10.1093/fsr/owae015]。
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引用次数: 0
Correction to: Forensic efficiency and population genetic construction of Guizhou Gelao minority from Southwest China revealed by a panel of 23 autosomal STR loci. Correction to:通过 23 个常染色体 STR 位点揭示中国西南贵州仡佬族的法医效率和种群遗传构建。
IF 1.4 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-10-17 eCollection Date: 2024-09-01 DOI: 10.1093/fsr/owae058

[This corrects the article DOI: 10.1093/fsr/owad058.].

[此处更正了文章 DOI:10.1093/fsr/owad058]。
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引用次数: 0
Correction to: Forensic features and phylogenetic structure survey of four populations from southwest China via the autosomal insertion/deletion markers. 更正为通过常染色体插入/缺失标记对中国西南地区四个种群的法医特征和系统发育结构进行调查。
IF 1.4 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-10-11 eCollection Date: 2024-09-01 DOI: 10.1093/fsr/owae059

[This corrects the article DOI: 10.1093/fsr/owad052.].

[此处更正了文章 DOI:10.1093/fsr/owad052]。
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引用次数: 0
Forensic identification in a multidisciplinary perspective focusing on big challenges. 多学科视角下的法医鉴定,聚焦重大挑战。
IF 1.4 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-09-30 eCollection Date: 2024-09-01 DOI: 10.1093/fsr/owae063
Eugénia Cunha, Zuzana Obertová
{"title":"Forensic identification in a multidisciplinary perspective focusing on big challenges.","authors":"Eugénia Cunha, Zuzana Obertová","doi":"10.1093/fsr/owae063","DOIUrl":"10.1093/fsr/owae063","url":null,"abstract":"","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"9 3","pages":"owae063"},"PeriodicalIF":1.4,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to: Potential role of the sella turcica X-ray imaging aspects for sex estimation in the field of forensic anthropology: a systematic review and meta-analysis. 更正:在法医人类学领域,X 射线成像在性别估计方面的潜在作用:系统回顾和荟萃分析。
IF 1.4 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-09-30 eCollection Date: 2024-09-01 DOI: 10.1093/fsr/owae060

[This corrects the article DOI: 10.1093/fsr/owad046.].

[此处更正了文章 DOI:10.1093/fsr/owad046]。
{"title":"Correction to: Potential role of the sella turcica X-ray imaging aspects for sex estimation in the field of forensic anthropology: a systematic review and meta-analysis.","authors":"","doi":"10.1093/fsr/owae060","DOIUrl":"https://doi.org/10.1093/fsr/owae060","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1093/fsr/owad046.].</p>","PeriodicalId":45852,"journal":{"name":"Forensic Sciences Research","volume":"9 3","pages":"owae060"},"PeriodicalIF":1.4,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11440701/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142356083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to: Evaluation of data collection bias of third molar stages of mineralisation for age estimation in the living. 更正:评估用于活人年龄估计的第三磨牙矿化阶段的数据收集偏差。
IF 1.4 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2024-09-27 eCollection Date: 2024-09-01 DOI: 10.1093/fsr/owae057

[This corrects the article DOI: 10.1093/fsr/owae004.].

[此处更正了文章 DOI:10.1093/fsr/owae004]。
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引用次数: 0
期刊
Forensic Sciences Research
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