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Oral health professional intervention and child physical abuse—European legal approach 口腔保健专业人员的干预和儿童身体虐待--欧洲法律方法
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2023-12-06 DOI: 10.1093/fsr/owad042
A. Corte-Real, P. Almiro, Mafalda Silva, T. Nunes, João Abreu, Carla Carreira, Duarte Nuno Vieira
Physical violence against children and adolescents is an issue of Global Public Health. This study aims to identify traumatic injuries and the medical–legal temporary framework of the victim’s profile in the European legal approach. Participants and Setting: The clinical reports of a Portuguese European Clinical Academic Center database were analyzed. An observational and prospective cohort study was performed. A descriptive analysis of the variables was conducted, considering gender, bimodal age groups, place of residence, offender data, place of occurrence, etiology, localization, type of injuries, personal injury assessment by Quantum doloris, and injury time. The statistical analysis was performed by Spearman’s rho and Kendall’s tau-b correlation tests, Pearson’s chi-square test of independence (χ2), and Mann–Whitney and Kruskal–Wallis nonparametric tests (P < 0.05). The relationship between age groups and the place of occurrence was statistically significant (P = 0.001). Orofacial and nonorofacial injuries were related (P = 0.035). The General Data Protection Regulation is not a barrier to the treatment and sharing of justified data but a framework for safeguarding individuals’ fundamental rights, including the Right to Health. Meticulous reporting of the clinical situation involves the victim, the occurrence, and the potential offender.
针对儿童和青少年的身体暴力是全球公共卫生的一个问题。本研究旨在确定创伤性伤害和欧洲法律方法中受害者概况的医疗-法律临时框架。参与者和环境:分析葡萄牙欧洲临床学术中心数据库的临床报告。进行了一项观察性和前瞻性队列研究。对变量进行描述性分析,考虑性别、双峰年龄组、居住地、罪犯数据、发生地点、病因、局部、损伤类型、量子损伤评估和损伤时间。统计学分析采用Spearman 's rho和Kendall 's tau-b相关检验、Pearson 's卡方独立性检验(χ2)、Mann-Whitney和Kruskal-Wallis非参数检验(P < 0.05)。不同年龄组与发病地点之间的关系有统计学意义(P = 0.001)。口面部损伤与非口面部损伤相关(P = 0.035)。《一般数据保护条例》不是处理和共享合理数据的障碍,而是保障个人基本权利,包括健康权的框架。细致的临床情况报告涉及受害者、事件发生和潜在的犯罪者。
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引用次数: 0
A bibliometric analysis of research trends for missing persons globally and in East Asia from 2000 to 2021 2000 年至 2021 年全球和东亚失踪人员研究趋势的文献计量分析
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2023-12-06 DOI: 10.1093/fsr/owad041
Zixuan Zeng, Ishmael Dikeledi, Yehui Lv
A bibliometric analysis was performed to explore the current research status and development trends for missing persons globally and in East Asia and to identify research hotspots and frontiers relating to this topic. A search was conducted to identify relevant literature on missing persons using the Web of Science Core Collection database for the period 2000–2021. Subsequently, a knowledge map was constructed using CiteSpace software to perform a visual analysis of the distribution of authors and institutions, journals, national/regional distribution; citation frequency; high-frequency keywords; and emerging research hotspots. The results showed firstly that discussions on missing persons and related topics in East Asia are held at the regional scale. There is a paucity of research on this topic, which has been conducted on a limited scale, lacks depth and possibly innovation and entails limited discussion in this region. Secondly, there is a lack of social science research on missing persons and related topics worldwide. Thirdly, relevant research in East Asia should continue to preserve its own characteristics, effectively addressing current issues and enabling more people to participate in social science-oriented discussions focusing on the topic of missing persons. This approach provides a promising direction for pursuing the sustainable development of the topic of missing persons.
通过文献计量分析,探讨全球和东亚地区失踪人口研究现状和发展趋势,并确定研究热点和前沿。使用Web of Science核心收集数据库检索2000-2021年期间关于失踪人口的相关文献。随后,利用CiteSpace软件构建知识图谱,对作者分布、机构分布、期刊分布、国家/地区分布进行可视化分析;引用频率;高频关键词;以及新兴的研究热点。结果表明:东亚地区失踪人口及相关议题的讨论是在区域尺度上进行的;关于这一主题的研究很少,研究范围有限,缺乏深度和创新,在本地区的讨论也很有限。其次,全球范围内对失踪人口及相关课题的社会科学研究缺乏。第三,东亚地区的相关研究应继续保持自身特点,有效解决当前问题,使更多的人参与到以失踪人口为主题的社会科学讨论中来。这种做法为追求失踪人员专题的可持续发展提供了一个有希望的方向。
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引用次数: 0
Sequence variations of 58 STRs and 94 SNPs in Northeastern Xibe with ForenSeq™ DNA signature prep kit 使用 ForenSeq™ DNA 特征预处理试剂盒分析锡伯族东北部 58 个 STR 和 94 个 SNP 的序列变化
IF 1.3 4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2023-12-06 DOI: 10.1093/fsr/owad043
Fei Guo, Longnian Zhang, Y. Xin, Shiquan Liu
Massively parallel sequencing (MPS) technology has already shown many advantages in forensic DNA typing. In this study, the repeat region (RR) and flanking region (FR) variations were detected at 58 short tandem repeats (STRs) by MPS, which could increase allelic diversity (34.59%) and gene diversity (3.95%) compared with the capillary electrophoresis (CE) method. Six novel RR variants were identified at 5 STRs, and eleven FR variants were found at 8 STRs. Concordance amounted to 99.96% (4598 out of 4600 alleles) between ForenSeq DNA Signature Prep Kit and CE kits, and discordance was due to bioinformatic misinterpretation at DXS7132. Additionally, allele frequencies and forensic parameters of 94 identity-informative single nucleotide polymorphisms (iiSNPs) provided a reference dataset, which held a higher power of discrimination (1–3.4825 × 10−35). A total of 29 FR variants were found at 24 target iiSNPs. The power of the system effectiveness with the combined 27 autosomal STRs by sequence and 94 iiSNPs was added more substantially than that of a single type of marker alone, such as the combined power of discrimination (CPD) and the combined power of exclusion (CPE) achieving 1–7.1358 × 10−65 and 1–4.4803 × 10−21, respectively. Population structure analyses with iiSNPs and additional ancestry-informative SNPs (aiSNPs) demonstrated that Chinese Xibe in the northeast was one of the East Asian populations. At the genetic level, Northeastern Xibe is close to populations where they live, such as Han Chinese and other ethnic minority groups.
大规模平行测序(MPS)技术在法医DNA分型中已经显示出许多优势。本研究利用MPS检测了58个短串联重复序列(STRs)的重复区(RR)和侧翼区(FR)变异,与毛细管电泳(CE)相比,该方法可提高等位基因多样性(34.59%)和基因多样性(3.95%)。在5个str中发现6个新的RR变异,在8个str中发现11个FR变异。法医DNA签名准备试剂盒与CE试剂盒之间的一致性达99.96%(4600个等位基因中有4598个),不一致是由于DXS7132的生物信息学误解。此外,94个身份信息单核苷酸多态性(iiSNPs)的等位基因频率和法医学参数提供了一个具有较高判别能力(1-3.4825 × 10−35)的参考数据集。在24个目标iiSNPs中共发现29个FR变异。与单独使用单个标记相比,将27个常染色体STRs序列和94个iiSNPs组合在一起的系统有效性增强更大,如联合辨别能力(CPD)和联合排除能力(CPE)分别达到1-7.1358 × 10−65和1-4.4803 × 10−21。利用iiSNPs和附加遗传信息性snp (aiSNPs)进行的种群结构分析表明,中国东北锡伯族是东亚人群之一。在遗传水平上,锡伯族东北部与他们居住的人群(如汉族和其他少数民族)接近。
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引用次数: 0
Sternal Rib Ends as a Method of Age Estimation at the CIL: A Brief Note 胸骨肋骨末端作为一种年龄估计方法:简要说明
4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2023-11-09 DOI: 10.3390/forensicsci3040041
Alexander F. Christensen
This communication reports the relationship between skeletal age estimates based on the sternal rib ends (using the phase method of İşcan and colleagues, later revised by Hartnett) and the chronological age at the death of 221 U.S. military casualties processed by the Scientific Analysis section of the Defense POW/MIA Accounting Agency and its predecessor laboratories from 2000 to the present. Previously published age ranges for each phase do not provide accurate estimates for a sufficient proportion of the cases for forensic use; as an example, the age ranges that accompany reference cast sets proved accurate for 55% of CIL cases. Combining the published age ranges of İşcan and Hartnett, on the other hand, proved accurate in 99% of the cases.
本文报告了根据胸骨肋骨末端估算的骨骼年龄(使用İşcan及其同事的相位法,后来由Hartnett修订)与国防部战俘/失踪人员会计局及其前身实验室从2000年至今处理的221名美军伤亡人员死亡时的实际年龄之间的关系。以前公布的每个阶段的年龄范围没有提供足够比例的法医案件的准确估计;例如,随参考铸型组而来的年龄范围证明对55%的CIL病例是准确的。另一方面,结合İşcan和Hartnett公布的年龄范围,证明99%的病例是准确的。
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引用次数: 0
Marijuana: Forensics of Abuse, Medical Uses, Controversy, and AI 大麻:滥用、医疗用途、争议和人工智能的法医学
4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2023-11-07 DOI: 10.3390/forensicsci3040040
Olen R. Brown
Motor vehicle accidents are a significant consequence of marijuana abuse. Limitations of its roadside detection and the forensic problems with establishing impairment require innovations that are scientifically achievable. Marijuana abuse currently is at an all-time high in the United States and its physiological effects make it a popular recreational drug that is reported to be a leading cause of preventable morbidity and mortality among the youth in the 18 most affluent nations. The medical benefits of drugs derived from marijuana complicate its forensic regulation. In an extensive 2017 report by The American Academy of Sciences, the evidence for the medical benefits of delta-9 tetrahydrocannabinol (derived from marijuana) were stated to be conclusive or substantial for the treatment of chronic pain in adults, as an antiemetic for treatment of chemotherapy-induced nausea and vomiting, and for improving patient-reported multiple sclerosis spasticity symptoms. The benefits from treatment for many other medical conditions were inconclusive or absent. The evidence is clear that safely driving a motor vehicle is significantly impaired while under the influence of marijuana. However, there is no roadside forensic test that reliably detects impairment, and there is an urgent need for such to protect the public while insuring the legal rights of users. There is strong societal pressure to relax marijuana’s forensic regulation, including removing it from Schedule I of the Controlled Substances Act. Artificial intelligence (AI) should be implemented with appropriate human control to resolve controversies.
机动车事故是滥用大麻的一个重要后果。其路边检测的局限性和确定损伤的法医问题需要科学上可以实现的创新。大麻的滥用目前在美国达到了历史最高水平,它的生理作用使它成为一种流行的娱乐毒品,据报道,在18个最富裕的国家中,大麻是导致年轻人可预防的发病和死亡的主要原因。从大麻中提取的药物的医疗效益使其司法监管复杂化。在美国科学院2017年的一份广泛报告中,delta-9四氢大麻酚(从大麻中提取)的医学益处的证据被认为是结论性的或实质性的,可用于治疗成人慢性疼痛,作为治疗化疗引起的恶心和呕吐的止吐剂,并改善患者报告的多发性硬化症痉挛症状。许多其他医疗条件的治疗所带来的好处是不确定的或不存在的。有证据表明,在大麻的影响下,安全驾驶机动车的能力明显受损。然而,目前还没有能够可靠地检测到损伤的路边法医测试,迫切需要这样的测试来保护公众,同时确保用户的合法权益。有强大的社会压力要求放松大麻的法医监管,包括将其从《受控物质法案》的附表一中删除。人工智能(AI)应该在适当的人为控制下实施,以解决争议。
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引用次数: 0
The Presence of the Human Auditory Ossicles—Detected Postmortem by CT Scan—As a Taphonomic Indicator 人听骨的存在-通过CT扫描检测死后-作为音标学指标
4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2023-11-02 DOI: 10.3390/forensicsci3040039
Edda E. Guareschi, Sara Poggesi, Marco Palmesino, Paola A. Magni
Introduction: Three tiny bones compose the human ossicular chain: malleus, incus and stapes. Also known as auditory ossicles, they are united by joints in the middle ear cavity of the petrous part of the temporal bone. Completely developed two years after birth, the ossicular chain is involved in the physiological process of hearing, by which sound waves from the environment are converted into electrochemical impulses. In the last 500 years, most studies have focused on the morphogenesis, morphological variability and clinical pathology of the ossicular chain, whilst only a few studies have added relevant knowledge to anthropology and forensic science. The auditory ossicles and the enclosing petrous bone are some of the hardest in the human skeleton. This is reflected in a relative resistance to fire and in the possibility of preservation and fossilization in millions of years. Materials and Methods: The literature and four present-day forensic cases were included in studying the postmortem loss of the auditory ossicles in skeletal or decomposing remains. Results indicate that it can be ascribed to their destruction or physical displacement, by either macro-micro-faunal action and/or any other natural or artificial disturbance. Discussion: Physical displacement is closely connected to the depositional environment of the skeletal remains, such as burial, entombment (sarcophagus, coffin, vault…), submersion or exposure to natural elements. Auditory ossicles can be recovered in situ, or very close to their anatomical location, when the skeletal material has been involved in an archaeological excavation. In the case of accessible or disturbed remains, scavengers may remove the tiny ossicles and/or they can slip out of the middle ear cavity following skull movements. Entombment offers effective protection against the displacement of the auditory ossicles, whereas aquatic submersion and aquatic movement almost invariably displace them. Conclusion: the preservation of the human auditory ossicles should be critically considered in the comprehensive context of any forensic investigation on human remains since it can assist the reconstruction of their taphonomic history. Taphonomic histories of remains can add crucial information to forensic investigations (e.g., the Post Mortem Interval, PMI). The aim of this study, limited by scarce relevant literature, is to discuss the potential role of the ossicular chain, detected by postmortem imaging techniques, as a taphonomical indicator in decomposing and/or skeletonized bodies.
人体听骨链由三块细小的骨头组成:锤骨、砧骨和镫骨。也被称为听小骨,它们由颞骨岩部中耳腔的关节连接。听骨链在出生两年后完全发育,它参与了听觉的生理过程,通过这个过程,来自环境的声波被转化为电化学脉冲。在过去的500年里,大多数研究都集中在听骨链的形态发生、形态变异和临床病理学上,而只有少数研究将相关知识添加到人类学和法医学中。听小骨和周围的石质骨是人类骨骼中最坚硬的部分。这反映在对火的相对抵抗力和数百万年保存和石化的可能性上。材料与方法:结合文献资料和四个现代法医案例,研究了骨骸或腐解遗骸中听小骨的死后损失。结果表明,这可归因于它们的破坏或物理位移,由宏观-微观动物作用和/或任何其他自然或人为干扰引起。讨论:物理位移与骨骼遗骸的沉积环境密切相关,如埋葬、埋葬(石棺、棺材、拱顶……)、淹没或暴露于自然因素。当骨骼材料参与考古挖掘时,可以在原位或非常接近其解剖位置的地方恢复听小骨。在可接近或干扰的遗骸的情况下,清除者可能会移除微小的听骨,并且/或者它们可以随着头骨运动而滑出中耳腔。埋在水中对听小骨的移位提供了有效的保护,而水中淹没和水中运动几乎总是会使听小骨移位。结论:人类听小骨的保存应在任何对人类遗骸进行法医调查的综合背景下进行批判性考虑,因为它可以帮助重建其地语学历史。遗骸的语音学历史可以为法医调查提供重要信息(例如,死后间隔,PMI)。由于缺乏相关文献,本研究的目的是讨论通过死后成像技术检测到的听骨链在分解和/或骨骼化的尸体中作为埋藏指标的潜在作用。
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引用次数: 0
ChatGPT in forensic sciences: a new Pandora’s box with advantages and challenges to pay attention ChatGPT在法医学中的应用:新的潘多拉魔盒,优势与挑战值得关注
4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2023-10-23 DOI: 10.1093/fsr/owad039
Ricardo Jorge Dinis-Oliveira, Rui M S Azevedo
Abstract ChatGPT is a variant of the GPT (Generative Pre-trained Transformer) language model that uses large amounts of text-based training data and a transformer architecture to generate human-like text adjusted to the received prompts. ChatGPT presents several advantages in forensic sciences, namely constituting a virtual assistant to aid lawyers, judges, and victims in managing and interpreting forensic expert data. But what would happen if ChatGPT began to be used to produce forensic expertise reports? Despite its potential applications, the use of ChatGPT and other Large Language Models (LLM) and artificial intelligence (AI) tools in forensic writing also poses ethical and legal concerns, which are discussed in this perspective together with some expected future perspectives.
ChatGPT是GPT (Generative Pre-trained Transformer)语言模型的一种变体,它使用大量基于文本的训练数据和转换器架构来生成根据收到的提示进行调整的类人文本。ChatGPT在法医学方面有几个优势,即构成一个虚拟助手,帮助律师、法官和受害者管理和解释法医专家数据。但是,如果ChatGPT开始用于生成法医专家报告,会发生什么呢?尽管有潜在的应用,在法医学写作中使用ChatGPT和其他大型语言模型(LLM)和人工智能(AI)工具也会带来道德和法律问题,我们将从这个角度以及一些预期的未来观点来讨论这些问题。
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引用次数: 0
A Reflective Spectroscopy and Mineralogical Investigation of Cosmetic Blush (Wet‘N’Wild) Potentially for Forensic Investigations Related to Interpersonal Violence—An Experimental Feasibility Study 化妆品腮红(湿的和野生的)的反射光谱和矿物学研究可能用于与人际暴力相关的法医调查-一项实验可行性研究
4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2023-10-17 DOI: 10.3390/forensicsci3040038
Juliana Curtis, Landon Stitle, Jessica Certain, Madeline Murchland, Charlotte Piszel, Jordan Vest, Claire L. McLeod, Mark P. S. Krekeler
Interpersonal violence is a rising issue in global society and new approaches are being sought to combat the problem. Within this context, expanding forensic techniques to better document violent crime scenes is critical for improving and acquiring legal evidence, such as proving or tracing contact between victims and suspects. This project aims to demonstrate the potential for forensic investigations in the context of interpersonal violence using a field-based reflective spectroscopy approach. For this, a common cosmetic, Wet‘N’Wild “Color Icon” blush in the shade “Pearlescent Pink”, was mineralogically characterized using transmission electron microscopy and powder X-ray diffraction and subsequently investigated via reflective spectroscopy on a variety of common substrates. Differing amounts of the cosmetic product, ranging from 0.001 g to 0.075 g, were applied to a variety of substrates using a simple push method to simulate forcible contact and material transfer. Substrates included a pine wood block; (calcareous) sand from Tulum, Mexico; Ottawa sand; tile; Pergo wood; linoleum; closet material; carpets; and fabrics. The reflective spectra of cosmetic–substrate combinations were measured via an ASD FieldSpec 4 Hi-Res spectroradiometer. The Wet‘N’Wild cosmetic was reliably detected on various substrates relevant to crime scenes. Minor amounts (as low as 0.02 mg/mm2) could be detected, and average limits of detection of 0.03 mg/mm2) were achieved; however, a calcareous sand (Tulum) had a high level of detection (>0.38 mg/mm2), suggesting that further investigation is needed for more complex sand substrates. The use of the ASD spectroradiometer as a forensic tool within the context of crime scene documentation shows promise. Future work should expand the characterization of cosmetic materials across a broad range of substrates and consider systematic studies of specific population groups. Furthermore, combining this approach with hyperspectral imaging at crime scenes is a promising future direction for crime scene documentation. This work therefore demonstrates a novel method for investigating cosmetics within the context of interpersonal violence and provides a foundation for future laboratory and field work using the ASD FieldSpec 4 and hyperspectral imaging systems.
人际暴力是全球社会中一个日益严重的问题,人们正在寻求新的方法来解决这个问题。在这种情况下,扩大法医技术以更好地记录暴力犯罪现场对于改进和获取法律证据至关重要,例如证明或追踪受害者与嫌疑人之间的接触。该项目旨在展示利用基于现场的反射光谱方法在人际暴力背景下进行法医调查的潜力。为此,一种常见的化妆品,Wet 'N 'Wild“Color Icon”腮红,在“珠光粉色”阴影下,使用透射电子显微镜和粉末x射线衍射进行矿物学表征,随后通过反射光谱在各种常见基材上进行研究。使用简单的推法将不同量的化妆品(从0.001 g到0.075 g)应用于各种基材上,以模拟强制接触和材料转移。基材包括松木块;(钙质)砂来自墨西哥图卢姆;渥太华砂;瓷砖;Pergo木材;油毡;衣柜材料;地毯;和面料。通过ASD fieldspec4高分辨率光谱辐射计测量了化妆品-衬底组合的反射光谱。在与犯罪现场相关的各种基材上可靠地检测到Wet 'N 'Wild化妆品。可以检测到少量(低至0.02 mg/mm2),平均检测限为0.03 mg/mm2);然而,钙质砂(Tulum)的检测水平很高(>0.38 mg/mm2),这表明需要对更复杂的砂基质进行进一步研究。在犯罪现场记录的背景下,使用ASD光谱辐射计作为法医工具显示了前景。未来的工作应该扩大化妆品材料在广泛的底物范围内的表征,并考虑对特定人群的系统研究。此外,将这种方法与犯罪现场的高光谱成像相结合,是犯罪现场记录的一个有希望的未来方向。因此,这项工作展示了一种在人际暴力背景下研究化妆品的新方法,并为未来使用ASD fieldspec4和高光谱成像系统的实验室和现场工作奠定了基础。
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引用次数: 0
miR-26a/30d/152 are reliable reference genes for miRNA quantification in skin wound age estimation miR-26a/30d/152是皮肤伤口年龄估计中miRNA定量的可靠参考基因
4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2023-10-15 DOI: 10.1093/fsr/owad037
Longlong Suo, Jian Cheng, Haomiao Yuan, Zhenf Fei Jiang, Dilichati Tash, Linlin Wang, Hao Cheng, Zhongduo Zhang, Fuyuan Zhang, Miao Zhang, Zhipeng Cao, Rui Zhao, Dawei Guan
Abstract MicroRNAs (miRNAs) are a class of small non-coding RNAs that exert their biological functions as negative regulators of gene expression. They are involved in the skin wound healing process with a dynamic expression pattern and can therefore potentially serve as biomarkers for skin wound age estimation. However, no reports have described any miRNAs as suitable reference genes (RGs) for miRNA quantification in wounded skin or samples with postmortem changes. Here, we aimed to identify specific miRNAs as RGs for miRNA quantification to support further studies of skin wound age estimation. Overall, nine miRNAs stably expressed in mouse skin at certain posttraumatic intervals (PTIs) were preselected by next-generation sequencing as candidate RGs. These nine miRNAs and the commonly used reference genes (comRGs: U6, GAPDH, ACTB,18S, 5S, LC-Ogdh) were quantitatively examined using qRT-PCR at different PTIs during skin wound healing in mice. The stabilities of these genes were evaluated using four independent algorithms: GeNorm, NormFinder, Bestkeeper, and comparative Delta Ct. Stability was further evaluated in mice with different postmortem intervals (PMIs). Overall, mmu-miR-26a-5p, mmu-miR-30d-5p, and mmu-miR-152-3p were identified as the most stable genes at both different PTIs and PMIs. These three miRNA RGs were additionally validated and compared with the comRGs in human samples. After assessing using one, two, or three miRNAs in combination for stability at different PTIs, PMIs, or in human samples, the set of miR-26a/30d/152 was approved as the best normalizer. In conclusion, our data suggest that the combination of miR-26a/30d/152 is recommended as the normalization strategy for miRNA qRT-PCR quantification in skin wound age estimation.
MicroRNAs (miRNAs)是一类小的非编码rna,作为基因表达的负调控因子发挥其生物学功能。它们以动态表达模式参与皮肤伤口愈合过程,因此可能作为皮肤伤口年龄估计的生物标志物。然而,没有报道描述任何miRNA作为合适的内参基因(RGs)来量化受伤皮肤或死后变化样本中的miRNA。在这里,我们旨在鉴定特定的miRNA作为RGs进行miRNA量化,以支持进一步研究皮肤伤口年龄的估计。总体而言,通过下一代测序预先选择了9个在小鼠皮肤中稳定表达的mirna作为候选RGs。采用qRT-PCR定量检测这9种mirna和常用内参基因(comgs: U6、GAPDH、ACTB、18S、5S、LC-Ogdh)在小鼠皮肤创面愈合过程中不同pti部位的表达情况。这些基因的稳定性使用四种独立的算法进行评估:GeNorm、NormFinder、Bestkeeper和comparative Delta Ct。在不同死后时间间隔(PMIs)的小鼠中进一步评估稳定性。总体而言,mmu-miR-26a-5p、mmu-miR-30d-5p和mmu-miR-152-3p被确定为在不同pti和pmi中最稳定的基因。对这三种miRNA RGs进行了进一步验证,并与人类样本中的comgs进行了比较。在评估了一种、两种或三种mirna组合在不同pti、pmi或人类样本中的稳定性后,miR-26a/30d/152被批准为最佳正态化剂。总之,我们的数据表明,miR-26a/30d/152的组合可作为皮肤伤口年龄估计中miRNA qRT-PCR定量的归一化策略。
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引用次数: 0
Exploring the Growing Importance of Forensic Geoarchaeology in Italy 探索法医地质考古学在意大利日益增长的重要性
4区 医学 Q3 MEDICINE, LEGAL Pub Date : 2023-10-10 DOI: 10.3390/forensicsci3040037
Pier Matteo Barone, Rosa Maria Di Maggio
Forensic geoarchaeology provides a comprehensive and interdisciplinary approach to investigating crime scenes. This field combines the techniques and methods of archaeology, geology, geophysics, and remote sensing, to provide a more comprehensive picture of the events that have occurred at a site. Forensic geoarchaeologists can not only provide valuable information about the context of a crime scene but also help reconstruct past events and collect relevant information about missing persons. Moreover, the use of geospatial technologies and the analysis of evidence together with a vast range of scientific methods and techniques help forensic geoarchaeologists provide compelling and reliable evidence before courts and law enforcement agencies. Although some reticence still prevails among Italians about recognizing forensic geoarchaeology as a fundamental discipline vis-a-vis crime scenes, the recognition of its vital significance is growing in Italy, thanks to the decision of the Italian Standards Organization (UNI) to standardize it alongside other fields of criminalistic expertise.
法医地质考古学为调查犯罪现场提供了一种全面和跨学科的方法。这个领域结合了考古学、地质学、地球物理学和遥感的技术和方法,提供了在一个地点发生的事件的更全面的画面。法医地质考古学家不仅可以提供有关犯罪现场背景的宝贵信息,还可以帮助重建过去的事件和收集失踪人员的相关信息。此外,地理空间技术的使用和证据分析以及广泛的科学方法和技术有助于法医地质考古学家向法院和执法机构提供令人信服和可靠的证据。尽管意大利人对将法医地质考古学作为犯罪现场研究的一门基础学科持保留态度,但由于意大利标准组织(UNI)决定将其与其他犯罪学专业领域一起标准化,意大利人越来越认识到法医地质考古学的重要意义。
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Forensic Sciences Research
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