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Patterns of proliferation and fibrosis in a rat model of endometriosis following administration of Allium cepa. 服用薤白后子宫内膜异位症大鼠模型的增殖和纤维化模式。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-06-01 Epub Date: 2024-01-24 DOI: 10.5653/cerm.2023.06261
Hakan Kula, Orkun Ilgen, Sefa Kurt, Filiz Yılmaz

Objective: Endometriosis is a common gynecological disease among reproductive-age women. Numerous hypotheses exist regarding the pathogenesis of endometriosis. In Turkey, the consumption of Allium cepa (commonly known as the "onion cure") is a popular treatment employed to alleviate a variety of gynecological disorders.

Methods: In this study, our objective was to assess the therapeutic mechanisms of the onion bulb A. cepa using an autologous endometriosis model in Sprague-Dawley rats. Previous research has shown that A. cepa possesses anti-inflammatory, antioxidant, and antiapoptotic properties. We evaluated the pathological condition of endometriotic implants by employing hematoxylin-eosin staining and Ki67 immunohistochemistry analysis. Transforming growth factor-beta 1 (TGF-β1) and alpha-smooth muscle actin (α-SMA) have been identified as profibrotic markers that are highly overexpressed in endometriotic tissues relative to eutopic endometrial tissue. Furthermore, TGF-β1 influences the differentiation and progression of endometriosis. To quantify profibrotic activity, we measured TGF-β1 and α-SMA using the immunosorbent assay method.

Results: Lower histologic evaluation scores for endometriotic implants were observed in the group receiving high-dose A. cepa relative to the other groups. Ki67 expression was reduced following the high-dose A. cepa regimen, which consisted of 30% A. cepa and 70% normal feed. However, no statistically significant differences in TGF-β1 or α-SMA levels were observed among the groups (p=0.7 and p=0.778, respectively).

Conclusion: The findings suggest that A. cepa could serve as a therapeutic agent in endometriosis treatment, as evidenced by the reduction in proliferative potential. Nevertheless, A. cepa was not associated with significantly lower levels of endometriosis-associated TGF-β1 or α-SMA.

目的:子宫内膜异位症是育龄妇女常见的妇科疾病:子宫内膜异位症是育龄妇女常见的妇科疾病。关于子宫内膜异位症的发病机理存在许多假说。在土耳其,食用薤白(俗称 "洋葱疗法")是缓解各种妇科疾病的常用疗法:在本研究中,我们的目的是利用自体子宫内膜异位症模型评估洋葱球茎 A. cepa 对 Sprague-Dawley 大鼠的治疗机制。先前的研究表明,洋葱头具有抗炎、抗氧化和抗细胞凋亡的特性。我们通过苏木精-伊红染色和 Ki67 免疫组化分析评估了子宫内膜异位植入物的病理状况。转化生长因子-β1(TGF-β1)和α-平滑肌肌动蛋白(α-SMA)已被确定为异位子宫内膜组织高度过表达的组织坏死标志物。此外,TGF-β1 还影响子宫内膜异位症的分化和进展。为了量化组织坏死活性,我们采用免疫吸附法测定了TGF-β1和α-SMA:结果:与其他组相比,接受大剂量牛肝菌治疗组的子宫内膜异位植入物组织学评估评分较低。大剂量牛肝菌治疗方案中,牛肝菌占 30%,正常饲料占 70%,Ki67 表达降低。然而,各组间的 TGF-β1 或 α-SMA 水平差异无统计学意义(分别为 p=0.7 和 p=0.778):结论:研究结果表明,牛肝菌可作为子宫内膜异位症的治疗药物,其增殖潜能的降低就证明了这一点。尽管如此,牛肝菌与子宫内膜异位症相关的 TGF-β1 或 α-SMA 水平的显著降低并无关联。
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引用次数: 0
Phycocyanin alleviates alcohol-induced testicular injury in male Wistar rats. 植物花青素可减轻酒精对雄性 Wistar 大鼠睾丸的损伤。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-06-01 Epub Date: 2024-01-17 DOI: 10.5653/cerm.2023.06422
Oumayma Boukari, Soumaya Ghoghbane, Wahid Khemissi, Thalja Lassili, Olfa Tebourbi, Khemais Ben Rhouma, Mohsen Sakly, Dorsaf Hallegue

Objective: Given the noteworthy implications of alcohol consumption and its association with male infertility, there has been a notable focus on investigating natural alternatives to mitigate its adverse effects. Thus, this study was conducted to assess the potential protective effect of phycocyanin extract derived from the blue algae Arthrospira (Spirulina) platensis against ethanol-induced oxidative stress, disturbances in testicular morphology, and alterations in sperm production.

Methods: Male rats were divided into four groups (five rats each): the control group received a saline solution, the ethanol exposed group (EtOH) was subjected to intraperitoneal injections of 10 mL/kg of ethanol solution at a concentration of 38% (v/v), the phycocyanin alone treated group (P) received oral administration of phycocyanin at a dosage of 50 mg/kg, and the phycocyanin-cotreated group (PE) was given oral phycocyanin followed by ethanol injections. All treatments were administered over a period of 14 days.

Results: Our findings demonstrated that ethanol exposure induced reproductive toxicity, characterized by reduced sperm production and viability, alterations in testicular weight and morphology, increased lipid peroxidation levels, and elevated oxidative enzyme activity. In addition, the ethanol-intoxicated group showed perturbations in serum biochemical parameters. However, the simultaneous exposure to ethanol and phycocyanin exhibited a counteractive effect against ethanol toxicity.

Conclusion: The results showed that supplementation of phycocyanin prevented oxidative and testicular morphological damage-induced by ethanol and maintained normal sperm production, and viability.

目的:鉴于饮酒的显著影响及其与男性不育症的关联,人们开始关注研究天然替代品来减轻酒精的不良影响。因此,本研究评估了从蓝藻节旋藻(螺旋藻)中提取的藻蓝蛋白提取物对乙醇引起的氧化应激、睾丸形态紊乱和精子生成改变的潜在保护作用:雄性大鼠分为四组(每组五只):对照组接受生理盐水,乙醇暴露组(EtOH)腹腔注射 10 mL/kg 浓度为 38% (v/v) 的乙醇溶液,单用植物蓝藻素处理组(P)口服植物蓝藻素,剂量为 50 mg/kg,植物蓝藻素协同处理组(PE)口服植物蓝藻素后再注射乙醇。所有处理均持续 14 天:结果:我们的研究结果表明,乙醇暴露会诱发生殖毒性,表现为精子产量和存活率降低、睾丸重量和形态改变、脂质过氧化水平升高以及氧化酶活性升高。此外,乙醇中毒组的血清生化指标也发生了变化。然而,同时摄入乙醇和植物花青素对乙醇毒性有对抗作用:结果表明,补充植物花青素可防止乙醇引起的氧化和睾丸形态损伤,并维持正常的精子生成和活力。
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引用次数: 0
Genomic aspects in reproductive medicine. 生殖医学的基因组方面。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-06-01 Epub Date: 2024-01-24 DOI: 10.5653/cerm.2023.06303
Minyeon Go, Sung Han Shim

Infertility is a complex disease characterized by extreme genetic heterogeneity, compounded by various environmental factors. While there are exceptions, individual genetic and genomic variations related to infertility are typically rare, often family-specific, and may serve as susceptibility factors rather than direct causes of the disease. Consequently, identifying the cause of infertility and developing prevention and treatment strategies based on these factors remain challenging tasks, even in the modern genomic era. In this review, we first examine the genetic and genomic variations associated with infertility, and subsequently summarize the concepts and methods of preimplantation genetic testing in light of advances in genome analysis technology.

不孕不育是一种复杂的疾病,其特点是遗传异质性极强,再加上各种环境因素的影响。虽然也有例外情况,但与不孕不育有关的个体遗传和基因组变异通常很少见,而且往往具有家族特异性,可作为易感因素而非直接病因。因此,即使在现代基因组时代,确定不孕不育的原因并根据这些因素制定预防和治疗策略仍然是一项具有挑战性的任务。在这篇综述中,我们首先探讨了与不孕症相关的基因和基因组变异,随后根据基因组分析技术的发展总结了胚胎植入前基因检测的概念和方法。
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引用次数: 0
Preservation of ovarian function using human pluripotent stem cell-derived mesenchymal progenitor cells. 利用源自人类多能干细胞的间充质祖细胞保护卵巢功能。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-05-24 DOI: 10.5653/cerm.2024.07003
Dong Ryul Lee, Jeoung Eun Lee

Ovarian reserve diminishes with age, and older women experience a corresponding shift in sex hormone levels. These changes contribute to an age-dependent decrease in fertility and a decline in overall health. Furthermore, while survival rates following cancer treatment have improved for young female patients, a reduction in ovarian function due to the side effects of such treatments can be difficult to avoid. To date, no effective therapy has been recommended to preserve ovarian health in these patients. Mesenchymal progenitor cells (MPCs) are considered a promising option for cell therapy aimed at maintaining fertility and fecundity. Although MPCs derived from human adult tissues are recognized for their various protective effects against ovarian senescence, they are limited in quantity. Consequently, human pluripotent stem cell-derived MPCs (hPSC-MPCs), which exhibit high proliferative capacity and retain genetic stability during growth, have been utilized to delay reproductive aging. This review highlights the impact of hPSC-MPCs on preserving the functionality of damaged ovaries in female mouse models subjected to chemotherapy and natural aging. It also proposes their potential as a valuable cell source for fertility preservation in women with a variety of diseases.

卵巢储备会随着年龄的增长而减少,老年妇女的性激素水平也会发生相应的变化。这些变化导致生育力随年龄而下降,整体健康水平也随之下降。此外,虽然年轻女性患者接受癌症治疗后的存活率有所提高,但很难避免因此类治疗的副作用而导致卵巢功能减退。迄今为止,还没有推荐任何有效的疗法来保护这些患者的卵巢健康。间充质祖细胞(MPCs)被认为是一种很有前景的细胞疗法,其目的是保持生育能力。虽然从人类成人组织中提取的间充质祖细胞被认为对卵巢衰老具有各种保护作用,但其数量有限。因此,人类多能干细胞衍生的多发性骨髓造血干细胞(hPSC-MPCs)被用于延缓生殖衰老,这种细胞具有高增殖能力,并在生长过程中保持遗传稳定性。这篇综述强调了 hPSC-MPCs 对受化疗和自然衰老影响的雌性小鼠模型中受损卵巢功能的保护作用。此外,它还提出了 hPSC-MPCs 作为一种宝贵的细胞来源,为患有各种疾病的妇女保留生育能力的潜力。
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引用次数: 0
The protective effect of zinc oxide and selenium oxide nanoparticles on the functional parameters of rat sperm during vitrification. 氧化锌和氧化硒纳米颗粒对大鼠精子玻璃化过程中功能参数的保护作用。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-03-01 Epub Date: 2023-12-01 DOI: 10.5653/cerm.2023.06079
Nafiseh Tavakolpoor Saleh, Zohreh Hosseinzadeh, Narges Gholami Banadkuki, Maryam Salehi Novin, Sanaz Saljooghi Zaman, Tohid Moradi Gardeshi

Objective: While sperm freezing (cryopreservation) is an effective method for preserving fertility, it can potentially harm the structure and function of sperm due to an increase in the production of reactive oxygen species. This study aimed to assess the impact of zinc oxide nanoparticles (ZnONPs) and selenium oxide nanoparticles (SeONPs) on various sperm functional parameters, including motility, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), acrosome membrane integrity (ACi), and malondialdehyde (MDA) levels.

Methods: Semen samples were collected from 20 Albino Wistar rats. These samples were then divided into six groups: fresh, cryopreservation control, and groups supplemented with SeONPs (1, 2, 5 μg/mL) and ZnONPs (0.1, 1, 10 μg/mL).

Results: Statistical analysis revealed that all concentrations of SeONPs increased total motility and progressive reduction of MDA levels compared to the cryopreservation control group (p<0.05). However, supplementation with ZnONPs did not affect these parameters (p>0.05). Conversely, supplements of 1 and 2 μg/mL SeONPs and 1 μg/mL ZnONPs contributed to the improvement of PMI and ACi (p<0.05). Yet, no significant change was observed in MMP with any concentration of SeONPs and ZnONPs compared to the cryopreservation control group (p>0.05).

Conclusion: The findings suggest that optimal concentrations of SeONPs may enhance sperm parameters during the freezing process.

目的:虽然精子冷冻(冷冻保存)是一种有效的保存生育能力的方法,但由于活性氧的产生增加,它可能会损害精子的结构和功能。本研究旨在评估氧化锌纳米颗粒(ZnONPs)和氧化硒纳米颗粒(SeONPs)对精子各种功能参数的影响,包括运动性、质膜完整性(PMI)、线粒体膜电位(MMP)、顶体膜完整性(ACi)和丙二醛(MDA)水平。方法:采集20只白化Wistar大鼠精液标本。将样品分为新鲜组、冷冻对照组和添加SeONPs组(1、2、5 μg/mL)和ZnONPs组(0.1、1、10 μg/mL)。结果:与低温保存对照组相比,各浓度SeONPs均使大鼠总运动性升高,MDA水平逐渐降低(p0.05)。相反,添加1、2 μg/mL SeONPs和1 μg/mL ZnONPs对PMI和ACi有改善作用(p0.05)。结论:最佳浓度的SeONPs可提高精子在冷冻过程中的各项参数。
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引用次数: 0
Effect of prior cesarean delivery on the outcomes of intracytoplasmic sperm injection. 剖腹产对卵胞浆内单精子注射结果的影响。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-03-01 Epub Date: 2024-01-12 DOI: 10.5653/cerm.2023.06163
Suzan Atteya Gewida, Mohamed Salah Eldeen Abd Rabbo, Mohammed Abd Elmoety El Samra, Hesham Mahmoud Adel Abdel Moneim

Objective: This study was conducted to investigate the impact of previous delivery mode on pregnancy outcomes in patients with secondary infertility after frozen-thawed embryo transfer.

Methods: This prospective observational study included 140 patients experiencing secondary infertility. Of these, 70 patients had a previous cesarean delivery (CD), while the remaining 70 patients had a previous normal vaginal delivery (NVD). The primary outcome was the implantation rate. The secondary outcomes included rates of clinical pregnancy, chemical pregnancy, miscarriage, and ectopic pregnancy.

Results: The comparison of all fertility outcomes between the two groups revealed no statistically significant differences. The implantation rate was 40.4% in the CD group and 41.7% in the NVD group (p=0.842). The clinical pregnancy rate was 50% in the CD group and 49.3% in the NVD group (p=0.932), while the chemical pregnancy rate was 14.6% in the CD group and 19% in the NVD group (p=0.591). The miscarriage rates in the CD and NVD groups were 20% and 17.6%, respectively (p=0.803). One case of tubal ectopic pregnancy occurred in the NVD group (1.4%).

Conclusion: The mode of prior delivery did not significantly impact pregnancy outcomes following frozen-thawed embryo transfer.

目的本研究旨在探讨冷冻解冻胚胎移植后,以往分娩方式对继发性不孕患者妊娠结局的影响:这项前瞻性观察研究纳入了 140 名继发性不孕患者。其中,70 名患者曾进行过剖宫产(CD),其余 70 名患者曾进行过正常阴道分娩(NVD)。主要结果是植入率。次要结果包括临床妊娠率、化学妊娠率、流产率和宫外孕率:两组所有生育结果的比较结果显示,差异无统计学意义。CD组的植入率为40.4%,NVD组为41.7%(P=0.842)。CD组的临床妊娠率为50%,NVD组为49.3%(P=0.932),而CD组的化学妊娠率为14.6%,NVD组为19%(P=0.591)。CD组和NVD组的流产率分别为20%和17.6%(P=0.803)。NVD组有一例输卵管异位妊娠(1.4%):结论:分娩方式对冷冻解冻胚胎移植后的妊娠结局没有明显影响。
{"title":"Effect of prior cesarean delivery on the outcomes of intracytoplasmic sperm injection.","authors":"Suzan Atteya Gewida, Mohamed Salah Eldeen Abd Rabbo, Mohammed Abd Elmoety El Samra, Hesham Mahmoud Adel Abdel Moneim","doi":"10.5653/cerm.2023.06163","DOIUrl":"10.5653/cerm.2023.06163","url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to investigate the impact of previous delivery mode on pregnancy outcomes in patients with secondary infertility after frozen-thawed embryo transfer.</p><p><strong>Methods: </strong>This prospective observational study included 140 patients experiencing secondary infertility. Of these, 70 patients had a previous cesarean delivery (CD), while the remaining 70 patients had a previous normal vaginal delivery (NVD). The primary outcome was the implantation rate. The secondary outcomes included rates of clinical pregnancy, chemical pregnancy, miscarriage, and ectopic pregnancy.</p><p><strong>Results: </strong>The comparison of all fertility outcomes between the two groups revealed no statistically significant differences. The implantation rate was 40.4% in the CD group and 41.7% in the NVD group (p=0.842). The clinical pregnancy rate was 50% in the CD group and 49.3% in the NVD group (p=0.932), while the chemical pregnancy rate was 14.6% in the CD group and 19% in the NVD group (p=0.591). The miscarriage rates in the CD and NVD groups were 20% and 17.6%, respectively (p=0.803). One case of tubal ectopic pregnancy occurred in the NVD group (1.4%).</p><p><strong>Conclusion: </strong>The mode of prior delivery did not significantly impact pregnancy outcomes following frozen-thawed embryo transfer.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"63-68"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139425704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Blastocyst formation in vitrified-warmed preimplantation embryos derived from vitrified-warmed oocytes in a mouse model. 在小鼠模型中,从玻璃化温育卵母细胞中提取的玻璃化温育植入前胚胎的囊胚形成。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-03-01 Epub Date: 2024-02-29 DOI: 10.5653/cerm.2023.06499
Yeon Hee Hong, Byung Chul Jee

Objective: The purpose of this study was to use a mouse model to investigate the blastocyst formation rate in vitrified-warmed embryos derived from vitrified-warmed oocytes.

Methods: Metaphase II oocytes obtained from BDF1 mice were vitrified and warmed, followed by fertilization with epididymal sperm. On day 3, a total of 176 embryos, at either the eight-cell or the morula stage, were vitrified-warmed (representing group 1). For group 2, 155 embryos at the same developmental stages were not vitrified, but rather were directly cultured until day 5. Finally, group 3 included day-5 blastocysts derived from fresh oocytes, which served as fresh controls. The primary outcome measured was the rate of blastocyst formation per day-3 embryo at the eight-cell or morula stage.

Results: The rates of blastocyst formation per day-3 embryo were comparable between groups 1 and 2, at 64.5% and 69.7%, respectively (p>0.05). The formation rates of good-quality blastocysts (expanded, hatching, or hatched) were also similar for groups 1 and 2, at 35.5% and 43.2%, respectively (p>0.05). For the fresh oocytes (group 3), the blastocyst formation rate was 75.5%, which was similar to groups 1 and 2. However, the rate of good-quality blastocyst formation in group 3 was 57.3%, significantly exceeding those of group 1 (p=0.001) and group 2 (p=0.023).

Conclusion: Regarding developmental potential to the blastocyst stage, vitrified-warmed day-3 embryos originating from vitrified-warmed oocytes demonstrated comparable results to non-vitrified embryos from similar oocytes. These findings indicate that day-3 embryos derived from vitrified-warmed oocytes can be effectively cryopreserved without incurring cellular damage.

研究目的本研究的目的是利用小鼠模型研究玻璃化温育卵母细胞所产生的玻璃化温育胚胎的囊胚形成率:方法:将从 BDF1 小鼠获得的二分裂期卵母细胞进行玻璃化和温育,然后用附睾精子受精。第 3 天,玻璃化-温育的胚胎共有 176 个,分别处于八细胞期或蜕膜期(代表第 1 组)。在第 2 组中,处于相同发育阶段的 155 个胚胎未进行玻璃化处理,而是直接培养至第 5 天。最后,第 3 组包括来自新鲜卵母细胞的第 5 天囊胚,作为新鲜对照组。测量的主要结果是每个第 3 天胚胎在 8 细胞或 morula 阶段的囊胚形成率:结果:第 1 组和第 2 组第 3 天胚胎的囊胚形成率相当,分别为 64.5%和 69.7%(P>0.05)。第 1 组和第 2 组的优质囊胚(膨大、孵化或孵化)形成率也相似,分别为 35.5% 和 43.2%(p>0.05)。新鲜卵母细胞(第 3 组)的囊胚形成率为 75.5%,与第 1 组和第 2 组相似。然而,第 3 组的优质囊胚形成率为 57.3%,明显高于第 1 组(p=0.001)和第 2 组(p=0.023):结论:在囊胚阶段的发育潜力方面,来自玻璃化温育卵母细胞的玻璃化温育第 3 天胚胎与来自类似卵母细胞的非玻璃化胚胎结果相当。这些研究结果表明,来自玻璃化温育卵母细胞的第 3 天胚胎可以有效冷冻保存而不会造成细胞损伤。
{"title":"Blastocyst formation in vitrified-warmed preimplantation embryos derived from vitrified-warmed oocytes in a mouse model.","authors":"Yeon Hee Hong, Byung Chul Jee","doi":"10.5653/cerm.2023.06499","DOIUrl":"10.5653/cerm.2023.06499","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to use a mouse model to investigate the blastocyst formation rate in vitrified-warmed embryos derived from vitrified-warmed oocytes.</p><p><strong>Methods: </strong>Metaphase II oocytes obtained from BDF1 mice were vitrified and warmed, followed by fertilization with epididymal sperm. On day 3, a total of 176 embryos, at either the eight-cell or the morula stage, were vitrified-warmed (representing group 1). For group 2, 155 embryos at the same developmental stages were not vitrified, but rather were directly cultured until day 5. Finally, group 3 included day-5 blastocysts derived from fresh oocytes, which served as fresh controls. The primary outcome measured was the rate of blastocyst formation per day-3 embryo at the eight-cell or morula stage.</p><p><strong>Results: </strong>The rates of blastocyst formation per day-3 embryo were comparable between groups 1 and 2, at 64.5% and 69.7%, respectively (p>0.05). The formation rates of good-quality blastocysts (expanded, hatching, or hatched) were also similar for groups 1 and 2, at 35.5% and 43.2%, respectively (p>0.05). For the fresh oocytes (group 3), the blastocyst formation rate was 75.5%, which was similar to groups 1 and 2. However, the rate of good-quality blastocyst formation in group 3 was 57.3%, significantly exceeding those of group 1 (p=0.001) and group 2 (p=0.023).</p><p><strong>Conclusion: </strong>Regarding developmental potential to the blastocyst stage, vitrified-warmed day-3 embryos originating from vitrified-warmed oocytes demonstrated comparable results to non-vitrified embryos from similar oocytes. These findings indicate that day-3 embryos derived from vitrified-warmed oocytes can be effectively cryopreserved without incurring cellular damage.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"51 1","pages":"57-62"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140022948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preferred strategy for euploid single embryo transfer in advanced maternal age: Fresh versus frozen. 高龄产妇优生单胚胎移植的首选策略:新鲜胚胎与冷冻胚胎
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-03-01 Epub Date: 2024-01-24 DOI: 10.5653/cerm.2023.06233
Fatma Ozdemir, Gokalp Oner, Semra Kahraman, Yucel Sahin, Hakan Yelke

Objective: The purpose of this study was to compare fresh and frozen-thawed euploid blastocyst transfer protocols following preimplantation genetic screening (PGS) in cases of advanced maternal age.

Methods: A total of 330 patients were examined retrospectively. PGS was performed on the embryos of 146 patients for whom fresh transfers were chosen. In contrast, frozen-thawed euploid single embryo transfer (ET) was selected after PGS for 184 patients, and their embryos were vitrified. The percentage of euploid embryos and rates of implantation, pregnancy, and pregnancy continuity, as well as clinical and biochemical abortion rates, were compared.

Results: The numbers of retrieved oocytes, metaphase II oocytes, and fertilized ova were greater in the frozen-thawed group. The percentages of euploid embryos were comparable between the fresh and frozen-thawed groups (32% vs. 34.8%, respectively). The rates of implantation (46.6%vs. 62.5%), pregnancy (50% vs. 66.8%), ongoing pregnancy (38.4% vs. 53.8%), and live birth percentage (37.0% vs. 53.8%) were significantly higher in the frozen-thawed group. However, no significant differences were found in the clinical and biochemical abortion rates.

Conclusion: The use of frozen-thawed single euploid ET is associated with increased implantation and pregnancy rates compared to fresh single euploid ET with PGS.

研究目的本研究的目的是比较高龄产妇植入前遗传学筛查(PGS)后新鲜和冷冻解冻的优倍囊胚移植方案:方法:共对 330 名患者进行了回顾性研究。对选择新鲜移植的 146 位患者的胚胎进行了 PGS。相比之下,184 位患者在进行 PGS 后选择了冷冻解冻的单胚胎移植(ET),并对其胚胎进行了玻璃化处理。结果比较了优倍体胚胎的比例、植入率、妊娠率和妊娠持续率,以及临床流产率和生化流产率:结果:冷冻解冻组取回的卵母细胞数、分裂期 II 卵母细胞数和受精卵数均多于冷冻解冻组。新鲜组和冷冻解冻组的优倍体胚胎比例相当(分别为 32% 和 34.8%)。冷冻解冻组的着床率(46.6% 对 62.5%)、妊娠率(50% 对 66.8%)、持续妊娠率(38.4% 对 53.8%)和活产率(37.0% 对 53.8%)显著高于新鲜组。然而,临床流产率和生化流产率没有发现明显差异:结论:与使用PGS的新鲜单倍体ET相比,使用冷冻解冻的单倍体ET可提高植入率和妊娠率。
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引用次数: 0
Detrimental impact of cell phone radiation on sperm DNA integrity. 手机辐射对精子 DNA 完整性的有害影响
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-03-01 Epub Date: 2024-01-24 DOI: 10.5653/cerm.2023.06121
Yeganeh Koohestanidehaghi, Mohammad Ali Khalili, Fatemeh Dehghanpour, Mohammad Seify

Radiofrequency electromagnetic radiation (RF-EMR) from various sources may impact health due to the generation of frequency bands. Broad pulses emitted within frequency bands can be absorbed by cells, influencing their function. Numerous laboratory studies have demonstrated that mobile phones-generally the most widely used devices-can have harmful effects on sex cells, such as sperm and oocytes, by producing RF-EMR. Moreover, some research has indicated that RF-EMR generated by mobile phones can influence sperm parameters, including motility, morphology, viability, and (most critically) DNA structure. Consequently, RF-EMR can disrupt both sperm function and fertilization. However, other studies have reported that exposure of spermatozoa to RF-EMR does not affect the functional parameters or genetic structure of sperm. These conflicting results likely stem from differences among studies in the duration and exposure distance, as well as the species of animal used. This report was undertaken to review the existing research discussing the effects of RF-EMR on the DNA integrity of mammalian spermatozoa.

各种来源的射频电磁辐射(RF-EMR)可能会因产生的频段而影响健康。频段内发射的宽脉冲可被细胞吸收,从而影响细胞的功能。大量实验室研究表明,移动电话(一般是使用最广泛的设备)会产生射频电磁辐射,对精子和卵细胞等性细胞产生有害影响。此外,一些研究表明,手机产生的射频-电磁辐射会影响精子参数,包括活力、形态、存活率和(最关键的)DNA 结构。因此,射频-电磁辐射会破坏精子的功能和受精能力。然而,其他研究报告称,精子暴露于射频-电磁辐射不会影响精子的功能参数或遗传结构。这些相互矛盾的结果很可能是由于不同的研究在持续时间和暴露距离以及所使用的动物种类上存在差异。本报告旨在回顾讨论射频-电磁辐射对哺乳动物精子 DNA 完整性影响的现有研究。
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引用次数: 0
Criteria for implementing artificial intelligence systems in reproductive medicine. 生殖医学人工智能系统实施标准。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-03-01 Epub Date: 2023-12-01 DOI: 10.5653/cerm.2023.06009
Enric Güell

This review article discusses the integration of artificial intelligence (AI) in assisted reproductive technology and provides key concepts to consider when introducing AI systems into reproductive medicine practices. The article highlights the various applications of AI in reproductive medicine and discusses whether to use commercial or in-house AI systems. This review also provides criteria for implementing new AI systems in the laboratory and discusses the factors that should be considered when introducing AI in the laboratory, including the user interface, scalability, training, support, follow-up, cost, ethics, and data quality. The article emphasises the importance of ethical considerations, data quality, and continuous algorithm updates to ensure the accuracy and safety of AI systems.

这篇综述文章讨论了人工智能(AI)在辅助生殖技术中的整合,并提供了将人工智能系统引入生殖医学实践时需要考虑的关键概念。文章重点介绍了人工智能在生殖医学中的各种应用,并讨论了是否使用商业或内部人工智能系统。本综述还提供了在实验室实施新的人工智能系统的标准,并讨论了在实验室引入人工智能时应考虑的因素,包括用户界面、可扩展性、培训、支持、后续、成本、道德和数据质量。文章强调了伦理考虑、数据质量和持续算法更新的重要性,以确保人工智能系统的准确性和安全性。
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引用次数: 0
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Clinical and Experimental Reproductive Medicine-CERM
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