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The differential expression patterns of Atg9a and Atg9b in cells of the reproductive organs. Atg9a和Atg9b在生殖器官细胞中的不同表达模式。
IF 1.8 Q2 Medicine Pub Date : 2024-05-17 DOI: 10.5653/cerm.2023.06737
Minseo Lee, Sujin Son, Hyunjung J Lim, Haengseok Song

Objective: Autophagy is a major intracellular catabolic pathway governed by the sequential actions of proteins encoded by autophagy-related genes (Atg). ATG9, the only transmembrane protein involved in this process, regulates phospholipid translocation to autophagosomes during the early phases of autophagy. In mammals, two Atg9 isoforms have been reported: Atg9a and Atg9b. In this study, we examined whether the molecular and cellular characteristics of these two isoforms differed in mice.

Methods: Whole uteri were collected on days 1, 4, and 8 of pregnancy and from ovariectomized mice injected with vehicle, progesterone, or 17β-estradiol. Cells from reproductive tissues, such as granulosa cells, uterine epithelial cells (UECs), uterine stromal cells (USCs), and oocytes were collected. Two human uterine cell lines were also used in this analysis. Reverse transcription-polymerase chain reaction tests, Western blotting, and immunofluorescence staining were performed. Serum starvation conditions were used to induce autophagy in primary cells.

Results: Atg9a and Atg9b were expressed in multiple mouse tissues and reproductive cells. Neither Atg9A nor Atg9B significantly changed in response to steroid hormones. Immunofluorescence staining of the UECs and USCs showed that ATG9A was distributed in a punctate-like pattern, whereas ATG9B exhibited a pattern of elongated tubular shapes in the cytoplasm. In human cancer cell lines, ATG9B was undetectable, whereas ATG9A was found in all cell types examined.

Conclusion: The Atg9 isoforms exhibited distinct subcellular localizations in UECs and may play different roles in autophagy. Notably, human uterine cells exhibited reduced ATG9B expression, suggesting that this suppression may be due to epigenetic regulation.

目的:自噬是一种主要的细胞内分解代谢途径,由自噬相关基因(Atg)编码的蛋白质依次发挥作用。ATG9 是参与这一过程的唯一跨膜蛋白,它在自噬的早期阶段调节磷脂向自噬体的转运。据报道,哺乳动物中有两种 Atg9 异构体:Atg9a和Atg9b。在这项研究中,我们考察了这两种异构体在小鼠体内的分子和细胞特征是否存在差异:方法:在小鼠妊娠第 1、4 和 8 天收集整个子宫,并对卵巢切除的小鼠注射药物、黄体酮或 17β-雌二醇。收集的细胞来自生殖组织,如颗粒细胞、子宫上皮细胞(UEC)、子宫基质细胞(USC)和卵母细胞。本分析还使用了两种人类子宫细胞系。研究人员进行了逆转录聚合酶链反应试验、免疫荧光染色。使用血清饥饿条件诱导原代细胞自噬:结果:Atg9a和Atg9b在多种小鼠组织和生殖细胞中表达。Atg9A和Atg9B对类固醇激素的反应均无明显变化。对UECs和USCs的免疫荧光染色显示,ATG9A呈点状分布,而ATG9B在细胞质中呈细长管状分布。在人类癌细胞系中,检测不到ATG9B,而在所有检测的细胞类型中都发现了ATG9A:结论:Atg9异构体在子宫内膜癌细胞中表现出不同的亚细胞定位,可能在自噬过程中发挥不同的作用。值得注意的是,人类子宫细胞的ATG9B表达量减少,这表明这种抑制可能是由于表观遗传调控所致。
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引用次数: 0
Oocyte cryopreservation for women with endometriosis: Justification, indications, and reproductive outcomes. 子宫内膜异位症妇女的卵母细胞冷冻:理由、适应症和生殖结果。
IF 1.8 Q2 Medicine Pub Date : 2024-05-07 DOI: 10.5653/cerm.2023.06492
Seung Joo Chon, Byung Chul Jee

Women with endometriosis often experience diminished ovarian reserve and a decreased number of oocytes retrieved. This reduction is exacerbated after surgery. Nevertheless, oocyte quality does not seem to be compromised in these patients. When embryos of good quality are obtained, in vitro fertilization outcomes are generally satisfactory. Oocyte cryopreservation may represent a fertility preservation option for women with planned and/or prior surgery, as it enables the collection of oocytes in advance. Given the diverse manifestations of endometriosis, which vary by type, age, and ovarian reserve, the decision to pursue oocyte cryopreservation should be weighed individually. Moreover, the potential benefits of this approach on future fertility must be carefully considered. Considering current guidelines, the most appropriate candidates for oocyte cryopreservation among women with endometriosis are: patients with bilateral endometriomas, typically larger than 3 cm; those with prior surgery for unilateral endometrioma who exhibit ipsilateral or contralateral recurrence; and those with unilateral endometrioma on a single ovary. However, the size criteria for endometrioma warrant further discussion. Conversely, oocyte cryopreservation is inadvisable for patients: with unilateral endometrioma smaller than 3 cm and good ovarian reserve; who have undergone surgery for bilateral endometriomas, regardless of recurrence; and who have diminished ovarian reserve. While consensus indicates that decisions regarding diminished ovarian reserve should be individualized, fertility preservation should often be considered for patients with serum anti-Müllerian hormone levels below 0.5 ng/mL. In such cases, a prolonged duration may be necessary to retrieve the desired 10 to 15 oocytes.

患有子宫内膜异位症的妇女通常会出现卵巢储备功能减退和获取卵母细胞数量减少的情况。手术后,这种减少的情况会更加严重。然而,这些患者的卵母细胞质量似乎并没有受到影响。如果能获得质量良好的胚胎,体外受精的结果一般都会令人满意。卵母细胞冷冻保存可以提前采集卵母细胞,因此对于计划和/或已经接受过手术的妇女来说,这可能是一种保留生育能力的选择。鉴于子宫内膜异位症的表现多种多样,且因类型、年龄和卵巢储备功能的不同而各异,因此在决定是否进行卵母细胞冷冻保存时,应进行单独权衡。此外,还必须仔细考虑这种方法对未来生育能力的潜在益处。根据目前的指南,子宫内膜异位症妇女中最适合进行卵母细胞冷冻保存的患者是:双侧子宫内膜异位症患者,通常大于 3 厘米;曾接受过单侧子宫内膜异位症手术且同侧或对侧复发者;单侧子宫内膜异位症位于单侧卵巢者。不过,子宫内膜异位症的大小标准还需要进一步讨论。相反,以下患者不宜进行卵母细胞冷冻保存:单侧子宫内膜瘤小于 3 厘米且卵巢储备功能良好者;曾接受过双侧子宫内膜瘤手术(无论是否复发)者;卵巢储备功能减退者。虽然共识表明,有关卵巢储备功能减退的决定应因人而异,但对于血清抗缪勒氏管激素水平低于 0.5 纳克/毫升的患者,通常应考虑保留生育能力。在这种情况下,可能需要延长取卵时间,以获取所需的 10 到 15 个卵母细胞。
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引用次数: 0
Comparative analysis of conventional in vitro fertilization and intracytoplasmic sperm injection in patients with polycystic ovarian syndrome, tubal factor infertility, and unexplained infertility whose partners exhibit normal semen parameters: a retrospective study of sibling oocytes. 对伴侣精液参数正常的多囊卵巢综合征、输卵管因素不孕和不明原因不孕患者进行常规体外受精和卵胞浆内单精子注射的比较分析:对同胞卵母细胞的回顾性研究。
IF 1.8 Q2 Medicine Pub Date : 2024-03-25 DOI: 10.5653/cerm.2023.06408
Sareh Ashourzadeh, Somayyeh Safari, Robabe Hosseinisadat, Raheleh Kafaeinezhad, Saeed Shokri, Sanaz Alaee

Objective: This study compared the outcomes of conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in patients with polycystic ovarian syndrome (PCOS), tubal factor (TF) infertility, and unexplained infertility whose partners had normal semen parameters.

Methods: This retrospective study included 360 couples diagnosed with infertility involving PCOS (n=157), unexplained infertility (n=140), and TF infertility (n=63). Sibling oocytes were randomly assigned to undergo ICSI or conventional IVF insemination. The fertilization rate and embryo morphology were evaluated as outcomes.

Results: Retrieved cumulus-oocyte complexes from patients with PCOS (2,974), unexplained infertility (1,843), and TF infertility (844) were split and inseminated by conventional IVF and ICSI respectively. In comparison to the ICSI method, the conventional IVF approach was linked to a significantly higher fertilization rate in groups with PCOS (68.81% vs. 77.49%), unexplained infertility (67.62% vs. 78.84%), and TF issues (69.23% vs. 78.63%) (p<0.05). The proportion of embryos with grade A produced by the conventional IVF method was significantly higher than that produced using the ICSI method in the PCOS and unexplained infertility groups (p<0.05). Additionally, the percentage of grade B embryos produced with the ICSI method was significantly higher than that produced with the conventional IVF method in PCOS patients (p=0.002).

Conclusion: Our results indicated that the conventional IVF method was associated with higher zygote production and a higher proportion of grade A embryos when all infertile groups were evaluated together. Thus, ICSI is not suggested for patients with these causes of infertility if their partner has normal semen parameters.

研究目的本研究比较了伴侣精液参数正常的多囊卵巢综合征(PCOS)、输卵管因素(TF)不孕症和不明原因不孕症患者的常规体外受精(IVF)和卵胞浆内单精子显微注射(ICSI)的结果:这项回顾性研究包括 360 对被诊断为多囊卵巢综合征(PCOS)(157 对)、不明原因不孕(140 对)和输卵管因素不孕(63 对)的不孕夫妇。同胞卵母细胞被随机分配接受卵胞浆内单精子显微注射(ICSI)或传统试管婴儿授精。结果对受精率和胚胎形态进行了评估:从多囊卵巢综合征(2974 例)、不明原因不孕症(1843 例)和 TF 不孕症(844 例)患者体内提取的精母细胞复合体进行了分离,并分别采用常规体外受精和卵胞浆内单精子显微注射进行授精。与卵胞浆内单精子显微注射法相比,常规体外受精法在多囊卵巢综合征(68.81% vs. 77.49%)、不明原因不孕(67.62% vs. 78.84%)和TF问题(69.23% vs. 78.63%)群体中的受精率明显更高(P结论:我们的研究结果表明,在对所有不孕症群体进行综合评估时,传统试管婴儿方法与较高的合子产量和较高的 A 级胚胎比例相关。因此,如果患者的配偶精液参数正常,则不建议对这些原因导致的不孕症患者采用卵胞浆内单精子显微注射法。
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引用次数: 0
Effect of prior cesarean delivery on the outcomes of intracytoplasmic sperm injection. 剖腹产对卵胞浆内单精子注射结果的影响。
IF 1.8 Q2 Medicine Pub Date : 2024-03-01 Epub Date: 2024-01-12 DOI: 10.5653/cerm.2023.06163
Suzan Atteya Gewida, Mohamed Salah Eldeen Abd Rabbo, Mohammed Abd Elmoety El Samra, Hesham Mahmoud Adel Abdel Moneim

Objective: This study was conducted to investigate the impact of previous delivery mode on pregnancy outcomes in patients with secondary infertility after frozen-thawed embryo transfer.

Methods: This prospective observational study included 140 patients experiencing secondary infertility. Of these, 70 patients had a previous cesarean delivery (CD), while the remaining 70 patients had a previous normal vaginal delivery (NVD). The primary outcome was the implantation rate. The secondary outcomes included rates of clinical pregnancy, chemical pregnancy, miscarriage, and ectopic pregnancy.

Results: The comparison of all fertility outcomes between the two groups revealed no statistically significant differences. The implantation rate was 40.4% in the CD group and 41.7% in the NVD group (p=0.842). The clinical pregnancy rate was 50% in the CD group and 49.3% in the NVD group (p=0.932), while the chemical pregnancy rate was 14.6% in the CD group and 19% in the NVD group (p=0.591). The miscarriage rates in the CD and NVD groups were 20% and 17.6%, respectively (p=0.803). One case of tubal ectopic pregnancy occurred in the NVD group (1.4%).

Conclusion: The mode of prior delivery did not significantly impact pregnancy outcomes following frozen-thawed embryo transfer.

目的本研究旨在探讨冷冻解冻胚胎移植后,以往分娩方式对继发性不孕患者妊娠结局的影响:这项前瞻性观察研究纳入了 140 名继发性不孕患者。其中,70 名患者曾进行过剖宫产(CD),其余 70 名患者曾进行过正常阴道分娩(NVD)。主要结果是植入率。次要结果包括临床妊娠率、化学妊娠率、流产率和宫外孕率:两组所有生育结果的比较结果显示,差异无统计学意义。CD组的植入率为40.4%,NVD组为41.7%(P=0.842)。CD组的临床妊娠率为50%,NVD组为49.3%(P=0.932),而CD组的化学妊娠率为14.6%,NVD组为19%(P=0.591)。CD组和NVD组的流产率分别为20%和17.6%(P=0.803)。NVD组有一例输卵管异位妊娠(1.4%):结论:分娩方式对冷冻解冻胚胎移植后的妊娠结局没有明显影响。
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引用次数: 0
The protective effect of zinc oxide and selenium oxide nanoparticles on the functional parameters of rat sperm during vitrification. 氧化锌和氧化硒纳米颗粒对大鼠精子玻璃化过程中功能参数的保护作用。
IF 1.8 Q2 Medicine Pub Date : 2024-03-01 Epub Date: 2023-12-01 DOI: 10.5653/cerm.2023.06079
Nafiseh Tavakolpoor Saleh, Zohreh Hosseinzadeh, Narges Gholami Banadkuki, Maryam Salehi Novin, Sanaz Saljooghi Zaman, Tohid Moradi Gardeshi

Objective: While sperm freezing (cryopreservation) is an effective method for preserving fertility, it can potentially harm the structure and function of sperm due to an increase in the production of reactive oxygen species. This study aimed to assess the impact of zinc oxide nanoparticles (ZnONPs) and selenium oxide nanoparticles (SeONPs) on various sperm functional parameters, including motility, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), acrosome membrane integrity (ACi), and malondialdehyde (MDA) levels.

Methods: Semen samples were collected from 20 Albino Wistar rats. These samples were then divided into six groups: fresh, cryopreservation control, and groups supplemented with SeONPs (1, 2, 5 μg/mL) and ZnONPs (0.1, 1, 10 μg/mL).

Results: Statistical analysis revealed that all concentrations of SeONPs increased total motility and progressive reduction of MDA levels compared to the cryopreservation control group (p<0.05). However, supplementation with ZnONPs did not affect these parameters (p>0.05). Conversely, supplements of 1 and 2 μg/mL SeONPs and 1 μg/mL ZnONPs contributed to the improvement of PMI and ACi (p<0.05). Yet, no significant change was observed in MMP with any concentration of SeONPs and ZnONPs compared to the cryopreservation control group (p>0.05).

Conclusion: The findings suggest that optimal concentrations of SeONPs may enhance sperm parameters during the freezing process.

目的:虽然精子冷冻(冷冻保存)是一种有效的保存生育能力的方法,但由于活性氧的产生增加,它可能会损害精子的结构和功能。本研究旨在评估氧化锌纳米颗粒(ZnONPs)和氧化硒纳米颗粒(SeONPs)对精子各种功能参数的影响,包括运动性、质膜完整性(PMI)、线粒体膜电位(MMP)、顶体膜完整性(ACi)和丙二醛(MDA)水平。方法:采集20只白化Wistar大鼠精液标本。将样品分为新鲜组、冷冻对照组和添加SeONPs组(1、2、5 μg/mL)和ZnONPs组(0.1、1、10 μg/mL)。结果:与低温保存对照组相比,各浓度SeONPs均使大鼠总运动性升高,MDA水平逐渐降低(p0.05)。相反,添加1、2 μg/mL SeONPs和1 μg/mL ZnONPs对PMI和ACi有改善作用(p0.05)。结论:最佳浓度的SeONPs可提高精子在冷冻过程中的各项参数。
{"title":"The protective effect of zinc oxide and selenium oxide nanoparticles on the functional parameters of rat sperm during vitrification.","authors":"Nafiseh Tavakolpoor Saleh, Zohreh Hosseinzadeh, Narges Gholami Banadkuki, Maryam Salehi Novin, Sanaz Saljooghi Zaman, Tohid Moradi Gardeshi","doi":"10.5653/cerm.2023.06079","DOIUrl":"10.5653/cerm.2023.06079","url":null,"abstract":"<p><strong>Objective: </strong>While sperm freezing (cryopreservation) is an effective method for preserving fertility, it can potentially harm the structure and function of sperm due to an increase in the production of reactive oxygen species. This study aimed to assess the impact of zinc oxide nanoparticles (ZnONPs) and selenium oxide nanoparticles (SeONPs) on various sperm functional parameters, including motility, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), acrosome membrane integrity (ACi), and malondialdehyde (MDA) levels.</p><p><strong>Methods: </strong>Semen samples were collected from 20 Albino Wistar rats. These samples were then divided into six groups: fresh, cryopreservation control, and groups supplemented with SeONPs (1, 2, 5 μg/mL) and ZnONPs (0.1, 1, 10 μg/mL).</p><p><strong>Results: </strong>Statistical analysis revealed that all concentrations of SeONPs increased total motility and progressive reduction of MDA levels compared to the cryopreservation control group (p<0.05). However, supplementation with ZnONPs did not affect these parameters (p>0.05). Conversely, supplements of 1 and 2 μg/mL SeONPs and 1 μg/mL ZnONPs contributed to the improvement of PMI and ACi (p<0.05). Yet, no significant change was observed in MMP with any concentration of SeONPs and ZnONPs compared to the cryopreservation control group (p>0.05).</p><p><strong>Conclusion: </strong>The findings suggest that optimal concentrations of SeONPs may enhance sperm parameters during the freezing process.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138463435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Blastocyst formation in vitrified-warmed preimplantation embryos derived from vitrified-warmed oocytes in a mouse model. 在小鼠模型中,从玻璃化温育卵母细胞中提取的玻璃化温育植入前胚胎的囊胚形成。
IF 1.8 Q2 Medicine Pub Date : 2024-03-01 Epub Date: 2024-02-29 DOI: 10.5653/cerm.2023.06499
Yeon Hee Hong, Byung Chul Jee

Objective: The purpose of this study was to use a mouse model to investigate the blastocyst formation rate in vitrified-warmed embryos derived from vitrified-warmed oocytes.

Methods: Metaphase II oocytes obtained from BDF1 mice were vitrified and warmed, followed by fertilization with epididymal sperm. On day 3, a total of 176 embryos, at either the eight-cell or the morula stage, were vitrified-warmed (representing group 1). For group 2, 155 embryos at the same developmental stages were not vitrified, but rather were directly cultured until day 5. Finally, group 3 included day-5 blastocysts derived from fresh oocytes, which served as fresh controls. The primary outcome measured was the rate of blastocyst formation per day-3 embryo at the eight-cell or morula stage.

Results: The rates of blastocyst formation per day-3 embryo were comparable between groups 1 and 2, at 64.5% and 69.7%, respectively (p>0.05). The formation rates of good-quality blastocysts (expanded, hatching, or hatched) were also similar for groups 1 and 2, at 35.5% and 43.2%, respectively (p>0.05). For the fresh oocytes (group 3), the blastocyst formation rate was 75.5%, which was similar to groups 1 and 2. However, the rate of good-quality blastocyst formation in group 3 was 57.3%, significantly exceeding those of group 1 (p=0.001) and group 2 (p=0.023).

Conclusion: Regarding developmental potential to the blastocyst stage, vitrified-warmed day-3 embryos originating from vitrified-warmed oocytes demonstrated comparable results to non-vitrified embryos from similar oocytes. These findings indicate that day-3 embryos derived from vitrified-warmed oocytes can be effectively cryopreserved without incurring cellular damage.

研究目的本研究的目的是利用小鼠模型研究玻璃化温育卵母细胞所产生的玻璃化温育胚胎的囊胚形成率:方法:将从 BDF1 小鼠获得的二分裂期卵母细胞进行玻璃化和温育,然后用附睾精子受精。第 3 天,玻璃化-温育的胚胎共有 176 个,分别处于八细胞期或蜕膜期(代表第 1 组)。在第 2 组中,处于相同发育阶段的 155 个胚胎未进行玻璃化处理,而是直接培养至第 5 天。最后,第 3 组包括来自新鲜卵母细胞的第 5 天囊胚,作为新鲜对照组。测量的主要结果是每个第 3 天胚胎在 8 细胞或 morula 阶段的囊胚形成率:结果:第 1 组和第 2 组第 3 天胚胎的囊胚形成率相当,分别为 64.5%和 69.7%(P>0.05)。第 1 组和第 2 组的优质囊胚(膨大、孵化或孵化)形成率也相似,分别为 35.5% 和 43.2%(p>0.05)。新鲜卵母细胞(第 3 组)的囊胚形成率为 75.5%,与第 1 组和第 2 组相似。然而,第 3 组的优质囊胚形成率为 57.3%,明显高于第 1 组(p=0.001)和第 2 组(p=0.023):结论:在囊胚阶段的发育潜力方面,来自玻璃化温育卵母细胞的玻璃化温育第 3 天胚胎与来自类似卵母细胞的非玻璃化胚胎结果相当。这些研究结果表明,来自玻璃化温育卵母细胞的第 3 天胚胎可以有效冷冻保存而不会造成细胞损伤。
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引用次数: 0
Preferred strategy for euploid single embryo transfer in advanced maternal age: Fresh versus frozen. 高龄产妇优生单胚胎移植的首选策略:新鲜胚胎与冷冻胚胎
IF 1.8 Q2 Medicine Pub Date : 2024-03-01 Epub Date: 2024-01-24 DOI: 10.5653/cerm.2023.06233
Fatma Ozdemir, Gokalp Oner, Semra Kahraman, Yucel Sahin, Hakan Yelke

Objective: The purpose of this study was to compare fresh and frozen-thawed euploid blastocyst transfer protocols following preimplantation genetic screening (PGS) in cases of advanced maternal age.

Methods: A total of 330 patients were examined retrospectively. PGS was performed on the embryos of 146 patients for whom fresh transfers were chosen. In contrast, frozen-thawed euploid single embryo transfer (ET) was selected after PGS for 184 patients, and their embryos were vitrified. The percentage of euploid embryos and rates of implantation, pregnancy, and pregnancy continuity, as well as clinical and biochemical abortion rates, were compared.

Results: The numbers of retrieved oocytes, metaphase II oocytes, and fertilized ova were greater in the frozen-thawed group. The percentages of euploid embryos were comparable between the fresh and frozen-thawed groups (32% vs. 34.8%, respectively). The rates of implantation (46.6%vs. 62.5%), pregnancy (50% vs. 66.8%), ongoing pregnancy (38.4% vs. 53.8%), and live birth percentage (37.0% vs. 53.8%) were significantly higher in the frozen-thawed group. However, no significant differences were found in the clinical and biochemical abortion rates.

Conclusion: The use of frozen-thawed single euploid ET is associated with increased implantation and pregnancy rates compared to fresh single euploid ET with PGS.

研究目的本研究的目的是比较高龄产妇植入前遗传学筛查(PGS)后新鲜和冷冻解冻的优倍囊胚移植方案:方法:共对 330 名患者进行了回顾性研究。对选择新鲜移植的 146 位患者的胚胎进行了 PGS。相比之下,184 位患者在进行 PGS 后选择了冷冻解冻的单胚胎移植(ET),并对其胚胎进行了玻璃化处理。结果比较了优倍体胚胎的比例、植入率、妊娠率和妊娠持续率,以及临床流产率和生化流产率:结果:冷冻解冻组取回的卵母细胞数、分裂期 II 卵母细胞数和受精卵数均多于冷冻解冻组。新鲜组和冷冻解冻组的优倍体胚胎比例相当(分别为 32% 和 34.8%)。冷冻解冻组的着床率(46.6% 对 62.5%)、妊娠率(50% 对 66.8%)、持续妊娠率(38.4% 对 53.8%)和活产率(37.0% 对 53.8%)显著高于新鲜组。然而,临床流产率和生化流产率没有发现明显差异:结论:与使用PGS的新鲜单倍体ET相比,使用冷冻解冻的单倍体ET可提高植入率和妊娠率。
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引用次数: 0
Detrimental impact of cell phone radiation on sperm DNA integrity. 手机辐射对精子 DNA 完整性的有害影响
IF 1.8 Q2 Medicine Pub Date : 2024-03-01 Epub Date: 2024-01-24 DOI: 10.5653/cerm.2023.06121
Yeganeh Koohestanidehaghi, Mohammad Ali Khalili, Fatemeh Dehghanpour, Mohammad Seify

Radiofrequency electromagnetic radiation (RF-EMR) from various sources may impact health due to the generation of frequency bands. Broad pulses emitted within frequency bands can be absorbed by cells, influencing their function. Numerous laboratory studies have demonstrated that mobile phones-generally the most widely used devices-can have harmful effects on sex cells, such as sperm and oocytes, by producing RF-EMR. Moreover, some research has indicated that RF-EMR generated by mobile phones can influence sperm parameters, including motility, morphology, viability, and (most critically) DNA structure. Consequently, RF-EMR can disrupt both sperm function and fertilization. However, other studies have reported that exposure of spermatozoa to RF-EMR does not affect the functional parameters or genetic structure of sperm. These conflicting results likely stem from differences among studies in the duration and exposure distance, as well as the species of animal used. This report was undertaken to review the existing research discussing the effects of RF-EMR on the DNA integrity of mammalian spermatozoa.

各种来源的射频电磁辐射(RF-EMR)可能会因产生的频段而影响健康。频段内发射的宽脉冲可被细胞吸收,从而影响细胞的功能。大量实验室研究表明,移动电话(一般是使用最广泛的设备)会产生射频电磁辐射,对精子和卵细胞等性细胞产生有害影响。此外,一些研究表明,手机产生的射频-电磁辐射会影响精子参数,包括活力、形态、存活率和(最关键的)DNA 结构。因此,射频-电磁辐射会破坏精子的功能和受精能力。然而,其他研究报告称,精子暴露于射频-电磁辐射不会影响精子的功能参数或遗传结构。这些相互矛盾的结果很可能是由于不同的研究在持续时间和暴露距离以及所使用的动物种类上存在差异。本报告旨在回顾讨论射频-电磁辐射对哺乳动物精子 DNA 完整性影响的现有研究。
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引用次数: 0
Criteria for implementing artificial intelligence systems in reproductive medicine. 生殖医学人工智能系统实施标准。
IF 1.8 Q2 Medicine Pub Date : 2024-03-01 Epub Date: 2023-12-01 DOI: 10.5653/cerm.2023.06009
Enric Güell

This review article discusses the integration of artificial intelligence (AI) in assisted reproductive technology and provides key concepts to consider when introducing AI systems into reproductive medicine practices. The article highlights the various applications of AI in reproductive medicine and discusses whether to use commercial or in-house AI systems. This review also provides criteria for implementing new AI systems in the laboratory and discusses the factors that should be considered when introducing AI in the laboratory, including the user interface, scalability, training, support, follow-up, cost, ethics, and data quality. The article emphasises the importance of ethical considerations, data quality, and continuous algorithm updates to ensure the accuracy and safety of AI systems.

这篇综述文章讨论了人工智能(AI)在辅助生殖技术中的整合,并提供了将人工智能系统引入生殖医学实践时需要考虑的关键概念。文章重点介绍了人工智能在生殖医学中的各种应用,并讨论了是否使用商业或内部人工智能系统。本综述还提供了在实验室实施新的人工智能系统的标准,并讨论了在实验室引入人工智能时应考虑的因素,包括用户界面、可扩展性、培训、支持、后续、成本、道德和数据质量。文章强调了伦理考虑、数据质量和持续算法更新的重要性,以确保人工智能系统的准确性和安全性。
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引用次数: 0
Clinical outcomes of preimplantation genetic testing for aneuploidy in high-risk patients: a retrospective cohort study. 高风险患者植入前非整倍体基因检测的临床结果:一项回顾性队列研究。
IF 1.8 Q2 Medicine Pub Date : 2024-03-01 Epub Date: 2023-12-27 DOI: 10.5653/cerm.2023.06394
Jun Woo Kim, So Young Lee, Chang Young Hur, Jin Ho Lim, Choon Keun Park

Objective: The purpose of this study was to evaluate the impact of preimplantation genetic testing for aneuploidy (PGT-A) on clinical outcomes among high-risk patients.

Methods: This retrospective study involved 1,368 patients and the same number of cycles, including 520 cycles with PGT-A and 848 cycles without PGT-A. The study participants comprised women of advanced maternal age (AMA) and those affected by recurrent implantation failure (RIF), recurrent pregnancy loss (RPL), or severe male factor infertility (SMF).

Results: PGT-A was associated with significant improvements in the implantation rate (IR) and the ongoing pregnancy rate/live birth rate (OPR/LBR) per embryo transfer cycle in the AMA (39.3% vs. 16.2% [p<0.001] and 42.0% vs. 21.8% [p<0.001], respectively), RIF (41.7% vs. 22.0% [p<0.001] and 47.0% vs. 28.6% [p<0.001], respectively), and RPL (45.6% vs. 19.5% [p<0.001] and 49.1% vs. 24.2% [p<0.001], respectively) groups, as well as the IR in the SMF group (43.3% vs. 26.5%, p=0.011). Additionally, PGT-A was associated with lower overall incidence rates of early pregnancy loss in the AMA (16.7% vs. 34.3%, p=0.001) and RPL (16.7% vs. 50.0%, p<0.001) groups. However, the OPR/LBR per total cycle across all PGT-A groups did not significantly exceed that for the non-PGT-A groups.

Conclusion: PGT-A demonstrated beneficial effects in high-risk patients. However, our findings indicate that these benefits are more pronounced in carefully selected candidates than in the entire high-risk patient population.

研究目的本研究旨在评估植入前非整倍体基因检测(PGT-A)对高风险患者临床结果的影响:这项回顾性研究涉及 1368 名患者和相同数量的周期,其中 520 个周期进行了 PGT-A,848 个周期未进行 PGT-A。研究参与者包括高龄产妇(AMA)和受复发性着床失败(RIF)、复发性妊娠丢失(RPL)或严重男性因素不育(SMF)影响的妇女:结果:PGT-A 能显著提高 AMA 患者每个胚胎移植周期的植入率(IR)和持续妊娠率/活产率(OPR/LBR)(39.3% vs. 16.2% [p结论:PGT-A 能显著提高 AMA 患者每个胚胎移植周期的植入率(IR)和持续妊娠率/活产率(OPR/LBR):PGT-A 对高风险患者有益处。然而,我们的研究结果表明,与整个高危患者群体相比,这些益处在精心挑选的候选者中更为明显。
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Clinical and Experimental Reproductive Medicine-CERM
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