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Effect of nicotinamide mononucleotide on osteogenesis in MC3T3-E1 cells against inflammation-induced by lipopolysaccharide. 烟酰胺单核苷酸对 MC3T3-E1 细胞成骨的影响,对抗脂多糖诱导的炎症。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-01 Epub Date: 2024-04-11 DOI: 10.5653/cerm.2023.06744
Inyoung Kang, Myoungjoo Koo, Jin Hyun Jun, Jaewang Lee

Objective: Nicotinamide mononucleotide (NMN) is extensively utilized as an anti-aging agent and possesses anti-inflammatory properties. Lipopolysaccharide (LPS) activates Toll-like receptor 4, a process modulated by intracellular signaling pathways such as the Wnt/β-catenin pathway. This study investigated the impact of NMN on osteogenesis in the presence of LPS.

Methods: To elucidate the role of NMN in osteogenesis in the context of Gram-negative bacterial infection after LPS treatment, we cultured a mouse pre-osteoblast cell line (MC3T3-E1) and subsequently incubated it with NMN and/or LPS. We then evaluated osteogenic activity by measuring alkaline phosphatase activity, assessing gene expression and protein levels, and performing Alizarin Red S staining and immunocytochemistry.

Results: MC3T3-E1 cells underwent successful differentiation into osteoblasts following treatment with osteogenic induction medium. LPS diminished features related to osteogenic differentiation, which were subsequently partially reversed by treatment with NMN. The restorative effects of NMN on LPS-exposed MC3T3-E1 cells were further substantiated by elucidating the role of Wnt/β-catenin signaling, as confirmed through immunocytochemistry.

Conclusion: This study showed that infection with Gram-negative bacteria disrupted the osteogenic differentiation of MC3T3-E1 cells. This adverse effect was partially reversed by administering a high-dose of NMN. Drawing on these results, we propose that NMN could serve as a viable therapeutic strategy to preserve bone homeostasis in elderly and immunocompromised patients.

目的:烟酰胺单核苷酸(NMN烟酰胺单核苷酸(NMN)被广泛用作抗衰老剂,并具有抗炎特性。脂多糖(LPS)可激活 Toll 样受体 4,这一过程受 Wnt/β-catenin 通路等细胞内信号通路的调节。本研究探讨了在 LPS 存在的情况下,NMN 对骨生成的影响:为了阐明在 LPS 处理后革兰氏阴性菌感染的情况下 NMN 在成骨过程中的作用,我们培养了小鼠前成骨细胞系(MC3T3-E1),随后用 NMN 和/或 LPS 对其进行培养。然后,我们通过测量碱性磷酸酶活性、评估基因表达和蛋白质水平以及进行茜素红 S 染色和免疫细胞化学分析来评估成骨活性:结果:经成骨诱导培养基处理后,MC3T3-E1细胞成功分化为成骨细胞。LPS 削弱了成骨分化的相关特征,而 NMN 的处理则部分逆转了这些特征。NMN对暴露于LPS的MC3T3-E1细胞的修复作用通过阐明Wnt/β-catenin信号传导的作用得到了进一步证实,这一点已通过免疫细胞化学得到证实:本研究表明,感染革兰氏阴性菌会破坏 MC3T3-E1 细胞的成骨分化。通过施用高剂量的 NMN 可部分逆转这种不利影响。根据这些结果,我们建议将 NMN 作为一种可行的治疗策略,以保护老年和免疫力低下患者的骨平衡。
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引用次数: 0
p,p'-DDT induces apoptosis in human endometrial stromal cells via the PI3K/AKT pathway and oxidative stress. p,p'-DDT通过PI3K/AKT途径和氧化应激诱导人子宫内膜基质细胞凋亡。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-01 Epub Date: 2024-05-07 DOI: 10.5653/cerm.2022.05792
So Ra Oh, Seung Bin Park, Yeon Jean Cho

Objective: Bis-[4-chlorophenyl]-1,1,1-trichloroethane (DDT), one of the most widely used synthetic pesticides, is an endocrine-disrupting chemical with the potential to interfere with the human reproductive system. The effects of DDT and one of its metabolites, p,p'-DDT, on human endometrial stromal cells (ESCs) and health outcomes remain unknown. In this study, we investigated whether p,p'-DDT induces an imbalance in cell proliferation and apoptosis in human ESCs via oxidative stress.

Methods: We assessed apoptosis in ESCs by quantifying the expression of markers associated with both intrinsic and extrinsic pathways. Additionally, we measured levels of reactive oxygen species (ROS), antioxidant enzyme activity, and estrogen receptors (ERs). We also examined changes in signaling involving nuclear factor kappa-light-chain-enhancer of activated B cells.

Results: Following treatment with 1,000 pg/mL of p,p'-DDT, we observed an increase in Bax expression, a decrease in Bcl-2 expression, and increases in the expression of caspases 3, 6, and 8. We also noted a rise in the generation of ROS and a reduction in glutathione peroxidase expression after treatment with p,p'-DDT. Additionally, p,p'-DDT treatment led to changes in ER expression and increases in the protein levels of phosphatidylinositol 3-kinase (PI3K), phospho-protein kinase B (phospho-AKT), and phospho-extracellular signal-regulated kinase (phospho-ERK).

Conclusion: p,p'-DDT was found to induce apoptosis in human ESCs through oxidative stress and an ER-mediated pathway. The activation of the PI3K/AKT and ERK pathways could represent potential mechanisms by which p,p'-DDT prompts apoptosis in human ESCs and may be linked to endometrial pathologies.

目的:双-[4-氯苯基]-1,1,1-三氯乙烷(DDT)是使用最广泛的合成杀虫剂之一,它是一种干扰内分泌的化学物质,有可能干扰人类生殖系统。DDT及其代谢物之一p,p'-DDT对人类子宫内膜基质细胞(ESC)和健康结果的影响仍然未知。在这项研究中,我们探讨了 p,p'-DDT 是否会通过氧化应激诱导人类 ESCs 细胞增殖和凋亡失衡:我们通过量化与内在和外在途径相关的标记物的表达来评估 ESCs 的细胞凋亡。此外,我们还测量了活性氧(ROS)、抗氧化酶活性和雌激素受体(ER)的水平。我们还检测了涉及活化 B 细胞的核因子卡巴轻链增强子的信号变化:结果:经 1,000 pg/mL p,p'-DDT 处理后,我们观察到 Bax 表达增加,Bcl-2 表达减少,caspases 3、6 和 8 表达增加。我们还注意到,p,p'-DDT 处理后,ROS 的生成增加,谷胱甘肽过氧化物酶的表达减少。此外,p,p'-DDT 处理导致ER表达发生变化,磷脂酰肌醇3-激酶(PI3K)、磷酸蛋白激酶B(phospho-AKT)和磷酸胞外信号调节激酶(phospho-ERK)的蛋白水平升高。PI3K/AKT和ERK通路的激活可能代表了p,p'-DDT促使人体间充质干细胞凋亡的潜在机制,并可能与子宫内膜病变有关。
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引用次数: 0
Systematic Review of the roles of Inositol and Vitamin D in improving fertility among patients with Polycystic Ovary Syndrome. 肌醇和维生素 D 在改善多囊卵巢综合征患者生育能力方面作用的系统性综述。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-09-01 Epub Date: 2024-04-11 DOI: 10.5653/cerm.2023.06485
Gitika Katyal, Gursharan Kaur, Hafsa Ashraf, Adiprasad Bodapati, Ayesha Hanif, Donatus Kaine Okafor, Safeera Khan

Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder among reproductive-age women. As a leading cause of anovulatory infertility, it complicates fertility treatments, including in vitro fertilization. The widely accepted 2003 Rotterdam diagnostic criteria for PCOS include sub-phenotypes based on variations in androgen excess, ovulatory dysfunction, and polycystic ovarian morphology. In this systematic review, we examined the impacts of inositol and vitamin D on fertility in PCOS. Adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 guidelines, we used relevant keywords to comprehensively search databases including PubMed, Google Scholar, and MDPI. From an initial pool of 345 articles, 10 met the inclusion criteria. The articles suggest that vitamin D and inositol, particularly myo-inositol and D-chiro-inositol, may represent therapeutic options for PCOS. Vitamin D influences ovarian follicular development, glucose regulation, and insulin sensitivity. When combined with metformin therapy, it is associated with improved menstrual regularity and ovulation. Inositol is crucial for cellular signaling, energy metabolism, glucose regulation, and fertility. This systematic review underscores the importance of investigating inositol and vitamin D within a PCOS management strategy, given the disorder's prevalence and impacts on fertility and metabolic health. Although these agents show promise, additional research could clarify their mechanisms of action and therapeutic benefits. This review emphasizes the need for exploration of effective treatments to improve the quality of life among individuals with PCOS. Inositol and vitamin D represent potential options, but more studies are required to elucidate their roles in the management of this condition.

多囊卵巢综合征(PCOS)是育龄妇女常见的内分泌和代谢紊乱疾病。多囊卵巢综合征是导致无排卵性不孕的主要原因之一,它使包括体外受精在内的生育治疗变得复杂。广泛接受的 2003 年鹿特丹多囊卵巢综合症诊断标准包括基于雄激素过多、排卵功能障碍和多囊卵巢形态变化的亚型。在本系统综述中,我们研究了肌醇和维生素 D 对多囊卵巢综合症患者生育力的影响。根据《2020 年系统综述和荟萃分析首选报告项目》指南,我们使用相关关键词对包括 PubMed、谷歌学术和 MDPI 在内的数据库进行了全面检索。在最初的 345 篇文章中,有 10 篇符合纳入标准。这些文章表明,维生素 D 和肌醇,尤其是肌醇和 D-chiro 肌醇,可能是多囊卵巢综合症的治疗选择。维生素 D 可影响卵巢卵泡的发育、血糖调节和胰岛素敏感性。与二甲双胍疗法结合使用时,可改善月经规律和排卵。肌醇对细胞信号传导、能量代谢、葡萄糖调节和生育能力至关重要。鉴于多囊卵巢综合症的发病率以及对生育和代谢健康的影响,本系统综述强调了在多囊卵巢综合症管理策略中研究肌醇和维生素 D 的重要性。尽管这些制剂显示出良好的前景,但更多的研究可以阐明它们的作用机制和治疗效果。本综述强调有必要探索有效的治疗方法,以改善多囊卵巢综合症患者的生活质量。肌醇和维生素 D 是潜在的选择,但还需要更多的研究来阐明它们在治疗这种疾病中的作用。
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引用次数: 0
Relationship between thyroid-stimulating hormone levels and the severity of vitamin D deficiency by age group. 各年龄组促甲状腺激素水平与维生素 D 缺乏症严重程度之间的关系。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-08-19 DOI: 10.5653/cerm.2023.06779
Mansi Modi, Pinky Garg

Objective: Researchers have long been captivated by the complex molecular interactions between vitamin D and the thyroid gland. Hypothyroidism affects 2% to 4% of women of reproductive age and can impact fertility through anovulatory cycles, luteal phase defects, hyperprolactinemia, and sex hormone imbalances. This study investigated the relationship between thyroid disease and the severity of vitamin D deficiency across different age groups.

Methods: A retrospective study was conducted of 286 patient samples from individuals aged 18 to 60 years who were processed in the clinical biochemistry laboratory of our hospital. Samples were tested for thyroid-stimulating hormone (TSH) and vitamin D (specifically, vitamin D3) levels. The study samples were categorized into four clinically relevant groups based on TSH levels and into three groups based on serum 25-hydroxyvitamin D (25(OH)D) levels.

Results: Most of the samples were from female patients (n=269), and the most common age group was 18 to 35 years (n=191, 66.78%). Subclinical hypothyroidism was identified in 120 patients, while vitamin D deficiency was present in 237 (82.87%) participants. A significant association was observed between vitamin D deficiency and the presence of thyroid disorders. Additionally, a significant negative correlation was found between TSH and vitamin D levels. Polycystic ovary syndrome was noted in 103 female patients (36.01%).

Conclusion: TSH and 25(OH)D levels should be screened in all women of reproductive age, not just those in high-risk groups, as subclinical and occult hypothyroidism may otherwise go undiagnosed. Furthermore, TSH should be considered the primary screening test.

研究目的长期以来,研究人员一直被维生素 D 与甲状腺之间复杂的分子相互作用所吸引。甲状腺功能减退症影响着2%至4%的育龄妇女,可通过无排卵周期、黄体期缺陷、高催乳素血症和性激素失衡影响生育能力。本研究调查了甲状腺疾病与不同年龄组维生素 D 缺乏严重程度之间的关系:我们对本院临床生化实验室处理的286份患者样本进行了回顾性研究,这些样本来自18至60岁的人群。对样本进行了促甲状腺激素(TSH)和维生素 D(特别是维生素 D3)水平检测。研究样本根据促甲状腺激素水平分为四个临床相关组,根据血清 25- 羟维生素 D(25(OH)D)水平分为三组:大多数样本来自女性患者(269 人),最常见的年龄组为 18 至 35 岁(191 人,66.78%)。在 120 名患者中发现了亚临床甲状腺功能减退症,而在 237 名参与者(82.87%)中发现了维生素 D 缺乏症。观察发现,维生素 D 缺乏与甲状腺疾病之间存在明显关联。此外,还发现促甲状腺激素和维生素 D 水平之间存在明显的负相关。103名女性患者(36.01%)患有多囊卵巢综合征:结论:所有育龄妇女都应接受促甲状腺激素和25(OH)D水平的筛查,而不仅仅是那些高危人群,因为亚临床和隐匿性甲状腺功能减退症可能会被漏诊。此外,促甲状腺激素应被视为主要的筛查项目。
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引用次数: 0
Therapeutic effects of curcumin nanoemulsion on cyclophosphamide-induced testicular toxicity in adult male mice. 姜黄素纳米乳剂对环磷酰胺诱导的成年雄性小鼠睾丸毒性的治疗作用
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-08-19 DOI: 10.5653/cerm.2024.07066
Pourya Raee, Shahin Aghamiri, Mahsa Ghaffari Novin, Azar Afshar, Fakhroddin Aghajanpour, Farid Abdi, Marefat Ghaffari Novin

Objective: Several chemotherapeutic agents, including cyclophosphamide (CP) and busulfan, have been shown to interfere with spermatogenesis. Accordingly, the main objective of this study was to evaluate the potential therapeutic effects of curcumin nanoemulsion (CUR-NE) on spermatogenesis in mice with CP-induced testicular toxicity.

Methods: A total of 28 adult male mice were equally divided into four groups: control, CUR-NE (30 mg/kg, daily for 5 weeks), CP (200 mg/kg, single dose), and CP+CUR-NE. Each group was evaluated regarding sperm parameters, DNA fragmentation index, chromatin maturation, reactive oxygen species (ROS) levels, and histological parameters of the testes. Serum levels of follicle-stimulating hormone (FSH), luteinizing hormone, and testosterone were also assessed in all groups.

Results: In CP-induced mice, CUR-NE treatment significantly improved sperm parameters, including total sperm count, motility, morphology, and DNA integrity. CUR-NE administration was also associated with significantly higher serum levels of testosterone and FSH, as well as testis weight and volume, in the mice treated with CP. Furthermore, CUR-NE treatment significantly increased the number of spermatogonia, primary spermatocytes, round spermatids, and Leydig cells in the testicular tissue of these animals. A marked reduction in ROS levels in the testes tissue was observed following administration of CUR-NE to CP-induced mice.

Conclusion: CUR-NE appears to promote spermatogenesis in mice with CP-induced testicular toxicity by reducing ROS levels, improving testicular stereological parameters, and strengthening the reproductive hormone profile.

目的:包括环磷酰胺(CP)和丁硫丹在内的几种化疗药物已被证明会干扰精子发生。因此,本研究的主要目的是评估姜黄素纳米乳剂(CUR-NE)对 CP 诱导的睾丸毒性小鼠精子发生的潜在治疗作用:将28只成年雄性小鼠平均分为四组:对照组、CUR-NE组(30毫克/千克,每天一次,连续5周)、CP组(200毫克/千克,单剂量)和CP+CUR-NE组。每组都对精子参数、DNA碎片指数、染色质成熟度、活性氧(ROS)水平和睾丸组织学参数进行了评估。此外,还评估了所有组的卵泡刺激素(FSH)、黄体生成素和睾酮的血清水平:结果:在CP诱导的小鼠中,CUR-NE能显著改善精子参数,包括精子总数、活力、形态和DNA完整性。服用 CUR-NE 还能显著提高 CP 治疗小鼠的睾酮和 FSH 血清水平,以及睾丸重量和体积。此外,CUR-NE 还能显著增加这些动物睾丸组织中精原细胞、初级精母细胞、圆形精子细胞和雷迪格细胞的数量。CP诱导的小鼠服用 CUR-NE 后,睾丸组织中的 ROS 水平明显下降:结论:CUR-NE 似乎能通过降低 ROS 水平、改善睾丸立体学参数和增强生殖激素谱来促进 CP 诱导的睾丸毒性小鼠的精子发生。
{"title":"Therapeutic effects of curcumin nanoemulsion on cyclophosphamide-induced testicular toxicity in adult male mice.","authors":"Pourya Raee, Shahin Aghamiri, Mahsa Ghaffari Novin, Azar Afshar, Fakhroddin Aghajanpour, Farid Abdi, Marefat Ghaffari Novin","doi":"10.5653/cerm.2024.07066","DOIUrl":"https://doi.org/10.5653/cerm.2024.07066","url":null,"abstract":"<p><strong>Objective: </strong>Several chemotherapeutic agents, including cyclophosphamide (CP) and busulfan, have been shown to interfere with spermatogenesis. Accordingly, the main objective of this study was to evaluate the potential therapeutic effects of curcumin nanoemulsion (CUR-NE) on spermatogenesis in mice with CP-induced testicular toxicity.</p><p><strong>Methods: </strong>A total of 28 adult male mice were equally divided into four groups: control, CUR-NE (30 mg/kg, daily for 5 weeks), CP (200 mg/kg, single dose), and CP+CUR-NE. Each group was evaluated regarding sperm parameters, DNA fragmentation index, chromatin maturation, reactive oxygen species (ROS) levels, and histological parameters of the testes. Serum levels of follicle-stimulating hormone (FSH), luteinizing hormone, and testosterone were also assessed in all groups.</p><p><strong>Results: </strong>In CP-induced mice, CUR-NE treatment significantly improved sperm parameters, including total sperm count, motility, morphology, and DNA integrity. CUR-NE administration was also associated with significantly higher serum levels of testosterone and FSH, as well as testis weight and volume, in the mice treated with CP. Furthermore, CUR-NE treatment significantly increased the number of spermatogonia, primary spermatocytes, round spermatids, and Leydig cells in the testicular tissue of these animals. A marked reduction in ROS levels in the testes tissue was observed following administration of CUR-NE to CP-induced mice.</p><p><strong>Conclusion: </strong>CUR-NE appears to promote spermatogenesis in mice with CP-induced testicular toxicity by reducing ROS levels, improving testicular stereological parameters, and strengthening the reproductive hormone profile.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142298341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Associations of dietary inflammatory indices (DII and E-DII) with sperm parameters. 膳食炎症指数(DII 和 E-DII)与精子参数的关系。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-08-01 DOI: 10.5653/cerm.2024.06982
Sonia Sadeghpour, Fatemeh Maleki Sedgi, Sevana Daneghian, Somayyeh Barania Adabi, Tahereh Behroozi-Lak, Mohammadreza Pashaei, Javad RasouIi, Rohollah Valizadeh, Hojat Ghasemnejad-Berenji

Objective: This study aimed to explore the ambiguous link between dietary inflammatory indices and sperm parameters. Specifically, it investigated the associations between the dietary inflammatory index (DII) and the energy-adjusted dietary inflammatory index (E-DII) with sperm motility, morphology, and count in men undergoing routine semen analysis.

Methods: A cross-sectional study was conducted with 144 men enrolled, where semen samples were collected and evaluated according to the 2010 World Health Organization guidelines. Dietary data were gathered using a 147-item semi-quantitative food frequency questionnaire developed by the researchers. Pearson correlation analysis was employed to assess the relationships of the DII and E-DII with sperm parameters.

Results: The mean DII and E-DII scores were 1.23±1.1 and 0.49±0.43, respectively. The mean values for sperm motility, morphology, and count were 43.08%±19.30%, 78.03%±26.99%, and 48.12±44.41 million, respectively. Both motility (r=-0.353) and count (r=-0.348) were found to be inversely and significantly correlated with DII. Similarly, Pearson correlation tests revealed strong and significant inverse correlations of motility (r=-0.389) and count (r=-0.372) with E-DII.

Conclusion: The findings suggest that a diet with a higher anti-inflammatory potential may be associated with increased sperm count and motility, but not with changes in morphology. Further research is necessary to confirm these findings, elucidate the underlying mechanisms, and identify dietary modifications that could improve male fertility.

研究目的本研究旨在探讨膳食炎症指数与精子参数之间的模糊联系。具体来说,它调查了接受常规精液分析的男性的膳食炎症指数(DII)和能量调整膳食炎症指数(E-DII)与精子活力、形态和数量之间的关系:这项横断面研究共有 144 名男性参加,根据 2010 年世界卫生组织指南收集和评估精液样本。研究人员使用自己编制的 147 项半定量食物频率问卷收集了饮食数据。采用皮尔逊相关分析评估 DII 和 E-DII 与精子参数的关系:结果:DII和E-DII的平均值分别为1.23±1.1和0.49±0.43。精子活力、形态和数量的平均值分别为 43.08%±19.30%、78.03%±26.99% 和 48.12±44.41 百万。研究发现,精子活力(r=-0.353)和精子数量(r=-0.348)与精子活力指数呈显著的反相关。同样,Pearson 相关性检验也显示,蠕动(r=-0.389)和计数(r=-0.372)与 E-DII 呈显著的反向相关:研究结果表明,抗炎潜力较高的饮食可能与精子数量和活力的增加有关,但与形态的变化无关。有必要开展进一步研究,以证实这些发现,阐明其潜在机制,并确定可提高男性生育能力的饮食调整方法。
{"title":"Associations of dietary inflammatory indices (DII and E-DII) with sperm parameters.","authors":"Sonia Sadeghpour, Fatemeh Maleki Sedgi, Sevana Daneghian, Somayyeh Barania Adabi, Tahereh Behroozi-Lak, Mohammadreza Pashaei, Javad RasouIi, Rohollah Valizadeh, Hojat Ghasemnejad-Berenji","doi":"10.5653/cerm.2024.06982","DOIUrl":"https://doi.org/10.5653/cerm.2024.06982","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to explore the ambiguous link between dietary inflammatory indices and sperm parameters. Specifically, it investigated the associations between the dietary inflammatory index (DII) and the energy-adjusted dietary inflammatory index (E-DII) with sperm motility, morphology, and count in men undergoing routine semen analysis.</p><p><strong>Methods: </strong>A cross-sectional study was conducted with 144 men enrolled, where semen samples were collected and evaluated according to the 2010 World Health Organization guidelines. Dietary data were gathered using a 147-item semi-quantitative food frequency questionnaire developed by the researchers. Pearson correlation analysis was employed to assess the relationships of the DII and E-DII with sperm parameters.</p><p><strong>Results: </strong>The mean DII and E-DII scores were 1.23±1.1 and 0.49±0.43, respectively. The mean values for sperm motility, morphology, and count were 43.08%±19.30%, 78.03%±26.99%, and 48.12±44.41 million, respectively. Both motility (r=-0.353) and count (r=-0.348) were found to be inversely and significantly correlated with DII. Similarly, Pearson correlation tests revealed strong and significant inverse correlations of motility (r=-0.389) and count (r=-0.372) with E-DII.</p><p><strong>Conclusion: </strong>The findings suggest that a diet with a higher anti-inflammatory potential may be associated with increased sperm count and motility, but not with changes in morphology. Further research is necessary to confirm these findings, elucidate the underlying mechanisms, and identify dietary modifications that could improve male fertility.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improved ovarian adiponectin system expression in polycystic ovary syndrome treated with exenatide. 用艾塞那肽治疗多囊卵巢综合征可改善卵巢脂肪生成素系统的表达。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-08-01 DOI: 10.5653/cerm.2024.06912
Asma Vatankhah, Mohabbat Jamhiri, Sima Vatankhah, Keivan Lorian, Mohammad Ebrahim Rezvani, Mahin Izadi

Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that can cause infertility. This experimental study was conducted to elucidate the role of adiponectin signaling in rats with PCOS treated with exenatide. Twenty-eight adult female Wistar rats were divided into four groups of seven. The normal group did not receive any drug. The PCOS+vehicle (Veh) group received estradiol valerate to induce PCOS, then was divided into PCOS +E50 and PCOS+E100 groups and treated with 50 or 100 mg/kg doses of exenatide, respectively. The mRNA expression of adiponectin and adiponectin receptor 1 (Adipo-R1) was evaluated using a semi-quantitative real-time polymerase chain reaction. The results indicated that the level of adiponectin diminished in the PCOS rats while exenatide increased adiponectin expression at both doses. Adiponectin receptor mRNA levels were higher in the PCOS rats than in the normal rats (p<0.05). In addition, exenatide decreased the levels of Adipo-R1 expression. Taken together, our results showed that exenatide may improve PCOS characteristics in rats through the molecular regulation of adiponectin and its receptor.

多囊卵巢综合征(PCOS)是一种常见的内分泌和代谢疾病,可导致不孕。本实验研究旨在阐明艾塞那肽治疗多囊卵巢综合征大鼠体内脂肪连接素信号转导的作用。28 只成年雌性 Wistar 大鼠被分为四组,每组 7 只。正常组不接受任何药物治疗。PCOS+车辆(Veh)组接受戊酸雌二醇诱导PCOS,然后分为PCOS+E50组和PCOS+E100组,分别给予50或100 mg/kg剂量的艾塞那肽治疗。采用半定量实时聚合酶链反应评估了脂肪连通素和脂肪连通素受体1(Adipo-R1)的mRNA表达。结果表明,在两种剂量下,PCOS 大鼠体内的脂肪生成素水平都有所下降,而艾塞那肽则增加了脂肪生成素的表达。多囊卵巢综合症大鼠的脂肪生成素受体 mRNA 水平高于正常大鼠(p
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引用次数: 0
Severe ovarian hyperstimulation syndrome in an oocyte donor despite preventive strategies. 尽管采取了预防策略,一名卵母细胞捐献者仍患上了严重的卵巢过度刺激综合征。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-07-22 DOI: 10.5653/cerm.2024.06884
Antonio Forgiarini, Mariana Selene Paredes Contreras, Silvia Bontá, Sara Maggi, Luis Alberto Quintero Espinel

We present a rare case of severe ovarian hyperstimulation syndrome (OHSS) in a 19-year-old woman undergoing a second donation cycle of controlled ovarian hyperstimulation. The patient developed severe OHSS despite the implementation of preventive strategies and required hospitalization for 14 days, including treatment in the intensive care unit. The underlying pathophysiology that triggers this extreme systemic response in certain patients, despite the implementation of preventive measures, remains unknown. Continued research efforts are necessary to improve our understanding and management of this condition.

我们报告了一例罕见的重度卵巢过度刺激综合征(OHSS)病例,患者是一名 19 岁女性,正在接受第二个捐赠周期的控制性卵巢过度刺激。尽管采取了预防策略,但患者仍出现了严重的卵巢过度刺激综合征,需要住院 14 天,包括在重症监护室接受治疗。尽管采取了预防措施,但引发某些患者出现这种极端全身反应的潜在病理生理学原因仍然不明。有必要继续开展研究,以提高我们对这种情况的理解和管理水平。
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引用次数: 0
Evaluation of the follicular fluid thiol/disulfide balance among patients with poor ovarian response. 评估卵巢反应不良患者卵泡液中硫醇/二硫化物的平衡。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-07-22 DOI: 10.5653/cerm.2024.06863
Esengul Türkyılmaz, Begün Erbaba, Salim Neşelioglu, Nafiye Karakaş Yılmaz, Özlem Moraloğlu Tekin

Objective: This study aimed to compare the thiol/disulfide balance, myeloperoxidase, and ischemia-modified albumin levels in the follicular fluid (FF) of poor ovarian response (POR) and normal ovarian response (NOR) women who received intracytoplasmic sperm injection (ICSI).

Methods: The study was performed between March 2021 and April 2022 at the Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Ankara City Hospital. The study included 27 POR and 35 NOR women who underwent ICSI. FF was obtained after the controlled ovarian stimulation cycle. The FF thiol/disulfide balance was detected using spectrophotometric methods. A correlation analysis was conducted to determine whether these oxidative stress markers could contribute to predicting oocyte quality.

Results: Disulfide levels were significantly higher in the NOR group than in the POR group (p=0.014). The number of fertilized egg (2PN) oocytes was positively correlated with the total thiol level (r=0.258, p=0.046). The disulfide level was positively correlated with the anti-Müllerian hormone level (r=0.262, p=0.039) and the total number of retrieved oocytes (r=0.335, p=0.008).

Conclusion: The disulfide levels differed significantly between the NOR and POR groups. The statistically significant differences of fewer metaphase II oocytes and lower percentage of good-quality embryos in the NOR group compared to the POR group might have resulted from the NOR group's elevated disulfide levels. The total thiol levels correlated with the total of 2PN oocytes. Future studies should examine the thiol/disulfide balance at assisted reproductive technology centers to predict which oocytes could be fertilized.

研究目的本研究旨在比较接受卵胞浆内单精子显微注射(ICSI)的卵巢反应不良(POR)和卵巢反应正常(NOR)女性卵泡液(FF)中的硫醇/二硫化物平衡、髓过氧化物酶和缺血修饰白蛋白水平:研究于 2021 年 3 月至 2022 年 4 月在安卡拉市医院生殖医学中心妇产科进行。研究对象包括 27 名 POR 和 35 名 NOR 妇女,她们都接受了卵胞浆内单精子显微注射。FF是在控制性卵巢刺激周期后获得的。采用分光光度法检测 FF 的硫醇/二硫化物平衡。进行了相关分析,以确定这些氧化应激标记物是否有助于预测卵母细胞质量:结果:NOR 组的二硫化物水平明显高于 POR 组(P=0.014)。受精卵(2PN)数量与总硫醇水平呈正相关(r=0.258,p=0.046)。二硫化物水平与抗缪勒氏管激素水平(r=0.262,p=0.039)和取卵细胞总数(r=0.335,p=0.008)呈正相关:结论:NOR 组和 POR 组的二硫化物水平差异显著。结论:NOR 组和 POR 组的二硫化物水平差异显著,NOR 组和 POR 组的二硫化物水平差异显著,可能是由于 NOR 组的二硫化物水平升高所致。总硫醇水平与 2PN 卵母细胞总数相关。未来的研究应检查辅助生殖技术中心的硫醇/二硫化物平衡情况,以预测哪些卵母细胞可以受精。
{"title":"Evaluation of the follicular fluid thiol/disulfide balance among patients with poor ovarian response.","authors":"Esengul Türkyılmaz, Begün Erbaba, Salim Neşelioglu, Nafiye Karakaş Yılmaz, Özlem Moraloğlu Tekin","doi":"10.5653/cerm.2024.06863","DOIUrl":"https://doi.org/10.5653/cerm.2024.06863","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to compare the thiol/disulfide balance, myeloperoxidase, and ischemia-modified albumin levels in the follicular fluid (FF) of poor ovarian response (POR) and normal ovarian response (NOR) women who received intracytoplasmic sperm injection (ICSI).</p><p><strong>Methods: </strong>The study was performed between March 2021 and April 2022 at the Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Ankara City Hospital. The study included 27 POR and 35 NOR women who underwent ICSI. FF was obtained after the controlled ovarian stimulation cycle. The FF thiol/disulfide balance was detected using spectrophotometric methods. A correlation analysis was conducted to determine whether these oxidative stress markers could contribute to predicting oocyte quality.</p><p><strong>Results: </strong>Disulfide levels were significantly higher in the NOR group than in the POR group (p=0.014). The number of fertilized egg (2PN) oocytes was positively correlated with the total thiol level (r=0.258, p=0.046). The disulfide level was positively correlated with the anti-Müllerian hormone level (r=0.262, p=0.039) and the total number of retrieved oocytes (r=0.335, p=0.008).</p><p><strong>Conclusion: </strong>The disulfide levels differed significantly between the NOR and POR groups. The statistically significant differences of fewer metaphase II oocytes and lower percentage of good-quality embryos in the NOR group compared to the POR group might have resulted from the NOR group's elevated disulfide levels. The total thiol levels correlated with the total of 2PN oocytes. Future studies should examine the thiol/disulfide balance at assisted reproductive technology centers to predict which oocytes could be fertilized.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Maternal exposure to phenanthrene induces testicular apoptosis and Sertoli cell dysfunction in F1 adult male mice: a histological and molecular study. 母体暴露于菲会诱发 F1 成年雄性小鼠睾丸凋亡和 Sertoli 细胞功能障碍:一项组织学和分子研究。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-07-22 DOI: 10.5653/cerm.2024.07038
Azar Afshar, Hamid Nazarian, Fatemeh Fadaefathabadi, Fakhroddin Aghajanpour, Reza Soltani, Mohammad-Amin Abdollahifar, Gholamreza Hassanzadeh, Mohsen Nourozian

Objective: Phenanthrene, a polycyclic aromatic hydrocarbon, is found in abundance in environmental pollutants, food, and drinking water. This substance can accumulate in body tissues and exert harmful effects. Moreover, phenanthrene can cross the placental barrier, potentially impacting fetal development. We aimed to explore the impacts of maternal exposure to phenanthrene on testicular tissue and Sertoli cell function in F1 mice.

Methods: Female rats with vaginal plugs were randomly assigned to one of three groups: control, sham, or phenanthrene. The control group received no intervention during pregnancy. In the sham and phenanthrene groups, corn oil and a phenanthrene solution, respectively, were administered via gavage once every 2 days. Offspring were separated by sex 21 days after birth. At 56 days postnatal, male F1 offspring were euthanized, and their testes were harvested for histological and molecular analyses.

Results: Phenanthrene exposure was associated with a lower testicular weight and volume, a smaller diameter of the seminiferous tubules, and a relative thinning of the germinal epithelium. These changes were associated with increased cellular apoptosis, as shown by the upregulation of caspase 3 expression. Additionally, we observed an increase in vacuolization and residual bodies within the tissue. Conversely, the number of Sertoli cells and expression levels of Sox9, as well as the Ocln and Itgb1 genes, were found to be lowered.

Conclusion: Maternal exposure to phenanthrene impacts both germ cells and Sertoli cells, disrupting their function and leading to fertility disorders in male F1 offspring mice.

目的:菲是一种多环芳烃,大量存在于环境污染物、食物和饮用水中。这种物质可在人体组织中蓄积并产生有害影响。此外,菲还能穿过胎盘屏障,对胎儿发育造成潜在影响。我们旨在探讨母体接触菲对 F1 小鼠睾丸组织和 Sertoli 细胞功能的影响:方法:将带有阴道塞的雌性大鼠随机分配到三组中的一组:对照组、假组或菲组。对照组在怀孕期间不接受任何干预。在假组和菲组中,每两天分别灌胃一次玉米油和菲溶液。后代在出生后 21 天按性别分开。在出生后 56 天,雄性 F1 后代被安乐死,并收获其睾丸进行组织学和分子分析:结果:接触菲后,睾丸重量和体积降低,曲细精管直径变小,生精上皮相对变薄。这些变化与细胞凋亡的增加有关,如 caspase 3 表达的上调。此外,我们还观察到组织内空泡化和残留体的增加。相反,我们发现 Sertoli 细胞的数量和 Sox9 以及 Ocln 和 Itgb1 基因的表达水平降低:结论:母体接触菲会影响生殖细胞和Sertoli细胞,破坏它们的功能,导致雄性F1后代小鼠出现生育障碍。
{"title":"Maternal exposure to phenanthrene induces testicular apoptosis and Sertoli cell dysfunction in F1 adult male mice: a histological and molecular study.","authors":"Azar Afshar, Hamid Nazarian, Fatemeh Fadaefathabadi, Fakhroddin Aghajanpour, Reza Soltani, Mohammad-Amin Abdollahifar, Gholamreza Hassanzadeh, Mohsen Nourozian","doi":"10.5653/cerm.2024.07038","DOIUrl":"https://doi.org/10.5653/cerm.2024.07038","url":null,"abstract":"<p><strong>Objective: </strong>Phenanthrene, a polycyclic aromatic hydrocarbon, is found in abundance in environmental pollutants, food, and drinking water. This substance can accumulate in body tissues and exert harmful effects. Moreover, phenanthrene can cross the placental barrier, potentially impacting fetal development. We aimed to explore the impacts of maternal exposure to phenanthrene on testicular tissue and Sertoli cell function in F1 mice.</p><p><strong>Methods: </strong>Female rats with vaginal plugs were randomly assigned to one of three groups: control, sham, or phenanthrene. The control group received no intervention during pregnancy. In the sham and phenanthrene groups, corn oil and a phenanthrene solution, respectively, were administered via gavage once every 2 days. Offspring were separated by sex 21 days after birth. At 56 days postnatal, male F1 offspring were euthanized, and their testes were harvested for histological and molecular analyses.</p><p><strong>Results: </strong>Phenanthrene exposure was associated with a lower testicular weight and volume, a smaller diameter of the seminiferous tubules, and a relative thinning of the germinal epithelium. These changes were associated with increased cellular apoptosis, as shown by the upregulation of caspase 3 expression. Additionally, we observed an increase in vacuolization and residual bodies within the tissue. Conversely, the number of Sertoli cells and expression levels of Sox9, as well as the Ocln and Itgb1 genes, were found to be lowered.</p><p><strong>Conclusion: </strong>Maternal exposure to phenanthrene impacts both germ cells and Sertoli cells, disrupting their function and leading to fertility disorders in male F1 offspring mice.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Clinical and Experimental Reproductive Medicine-CERM
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