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Contribution of chromosomal aberrations to the pathogenesis of primary and secondary amenorrhea: A study from Western Iran. 染色体畸变对原发性和继发性闭经发病机制的影响:伊朗西部的一项研究。
IF 1.8 Q3 OBSTETRICS & GYNECOLOGY Pub Date : 2024-07-22 DOI: 10.5653/cerm.2024.06807
Azam Azimi, Matin Heidari, Reza Alibakhshi, Sara Hamani, Negar Salimi, Parham Nejati, Nazanin Jalilian

Objective: Amenorrhea is an abnormal condition characterized by the absence of menstruation in women of reproductive age. According to the World Health Organization, amenorrhea ranks as the sixth leading cause of female infertility. Approximately 2% to 5% of women of reproductive age experience amenorrhea, which can be classified as primary amenorrhea (PA) or secondary amenorrhea (SA). Several studies have named chromosomal abnormalities among the main causes of amenorrhea, though the prevalence of these abnormalities may differ across populations. The objective of this study was to ascertain the frequency and types of chromosomal abnormalities in women with amenorrhea in Kermanshah Province, Iran.

Methods: This retrospective study included patients with PA and SA who underwent standard cytogenetic analysis. We also conducted a review of the literature on chromosomal abnormalities and their prevalence in SA.

Results: Among the 137 cases of PA in this study, 22% exhibited chromosomal abnormalities. Numerical changes were the most common finding (46.6%) in this group, including 45,X, mosaic, and 47,XXX karyotypes. These were followed by the 46,XY karyotype (40%). Of the 51 cases of SA that received chromosomal analysis, abnormalities were identified in only one case. Additionally, our review of the literature revealed that chromosomal aberrations are responsible for 7% of SA cases globally.

Conclusion: In this study, we successfully characterized the cytogenetic causes of PA and SA in a substantial population from Kermanshah Province, Iran.

目的:闭经是育龄妇女没有月经的一种异常情况。据世界卫生组织统计,闭经是导致女性不孕的第六大原因。约有 2%至 5%的育龄妇女出现闭经,可分为原发性闭经(PA)和继发性闭经(SA)。一些研究指出,染色体异常是导致闭经的主要原因之一,但这些异常的发生率在不同人群中可能有所不同。本研究旨在确定伊朗克尔曼沙阿省闭经妇女中染色体异常的频率和类型:这项回顾性研究纳入了接受标准细胞遗传学分析的 PA 和 SA 患者。我们还回顾了有关染色体异常及其在 SA 中发病率的文献:结果:在本研究的 137 例 PA 患者中,22% 表现出染色体异常。数字变化是这组病例中最常见的发现(46.6%),包括 45,X、马赛克和 47,XXX 核型。其次是 46,XY 核型(40%)。在 51 例接受染色体分析的 SA 患者中,只有一例发现异常。此外,我们的文献综述显示,全球 7% 的 SA 病例是由染色体畸变引起的:在这项研究中,我们成功鉴定了伊朗克尔曼沙阿省大量人群中 PA 和 SA 的细胞遗传学病因。
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引用次数: 0
Osteocalcin improves testicular morphology but does not ameliorate testosterone synthesis signaling in azoospermic mice. 骨钙素能改善无精症小鼠的睾丸形态,但不能改善睾酮合成信号。
IF 1.8 Q2 Medicine Pub Date : 2024-06-10 DOI: 10.5653/cerm.2023.06674
Mahsa Yaghobinejad, Heidar Toolee, Somayeh Solhjoo, Elham Seifali, Soraya Parvari, Omotosho Dhulqarnain Akanji, Tayebeh Rastegar

Objective: Osteocalcin (OCN) influences spermatogenesis in conjunction with testosterone and estrogen. OCN facilitates the secretion of testosterone by engaging with G protein-coupled receptor class C group 6 member A (GPRC6A) on Leydig cells and with androgen receptors on Sertoli cells.

Methods: Adult mice were assigned to the following groups: control; sham I, which received dimethyl sulfoxide for 5 weeks followed by phosphate-buffered saline for 1 month; azoospermia, which was treated with busulfan (40 mg/kg); sham II, which consisted of azoospermic animals that received phosphate-buffered saline for 1 month beginning at the 5-week mark; and the experimental group, which included azoospermic mice treated with OCN (3 ng/g/day) for 1 month.

Results: In the mice receiving OCN treatment, immunohistochemical analysis revealed increased expression of androgen receptors and GPRC6A, indicative of enhanced spermatogenesis. Additionally, the expression levels of the cyclic adenosine monophosphate-responsive element binding protein 1, steroidogenic acute regulatory protein, and cytochrome P450 family 11 genes were elevated. However, testosterone levels exhibited no significant differences across groups. Morphometric analysis suggests that OCN may play a crucial role in spermatogenesis, as evidenced by its positive effects on germinal cells and the germinal epithelium in the azoospermia group (p<0.05).

Conclusion: We conclude that OCN may serve as a beneficial therapeutic agent for male infertility.

目的骨钙素(OCN)与睾酮和雌激素共同影响精子发生。OCN通过与Leydig细胞上的G蛋白偶联受体C类6组A成员(GPRC6A)和Sertoli细胞上的雄激素受体结合,促进睾酮的分泌:成年小鼠被分为以下几组:对照组;假I组,接受二甲基亚砜治疗5周,然后接受磷酸盐缓冲盐水治疗1个月;无精子症组,接受丁硫酚(40毫克/千克)治疗;假II组,包括无精子症动物,从5周开始接受磷酸盐缓冲盐水治疗1个月;实验组,包括接受OCN(3纳克/克/天)治疗1个月的无精子症小鼠:结果:在接受 OCN 治疗的小鼠中,免疫组化分析显示雄激素受体和 GPRC6A 的表达增加,表明精子发生增强。此外,环腺苷酸单磷酸反应元件结合蛋白 1、类固醇生成急性调节蛋白和细胞色素 P450 家族 11 基因的表达水平也有所提高。不过,睾酮水平在各组之间没有明显差异。形态计量分析表明,OCN 对无精子症组的生精细胞和生精上皮细胞有积极影响(p 结论:OCN 可能在精子发生过程中发挥关键作用:我们得出的结论是,OCN 可作为治疗男性不育症的一种有益药物。
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引用次数: 0
Effect of treatment of chronic hepatitis c virus patients with direct-acting anti-retroviral drugs on semen and hormonal parameters. 用直接作用抗逆转录病毒药物治疗慢性丙型肝炎病毒患者对精液和激素参数的影响。
IF 1.8 Q2 Medicine Pub Date : 2024-06-10 DOI: 10.5653/cerm.2023.06772
Yosra H Mahmoud, Basem Eysa, Eman Mohamed Salah Ahmed, Heba Abdelaziz, Ashgan Mohamed Zayed, Amin Abdel Baki, Ahmed Hosny, Mohamed Hassany

Objective: Hepatitis C virus (HCV) infection is known to influence the seminal and hormonal parameters of infected men. This study was performed to assess the effects of HCV clearance using direct-acting antiviral (DAA) agents on semen and hormonal parameters.

Methods: A total of 50 patients with chronic HCV were enrolled, and conventional semen analysis was performed according to World Health Organization guidelines. Basal levels of total testosterone, free testosterone (FT), follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), prolactin, and sex hormone-binding globulin (SHBG) were assessed before and 3 months after treatment with DAAs.

Results: Following DAA treatment, statistically significant increases were observed in sperm motility and the proportion of grade A sperm. Additionally, the percentage of abnormal forms was significantly decreased after treatment (p=0.000). However, no significant differences were observed in semen volume, concentration, or total sperm count. Sex hormone analysis of patients after DAA treatment revealed significant increases in FT, LH, and FSH levels, along with significant decreases in SHBG, prolactin, and E2 levels.

Conclusion: Following HCV clearance, we noted an improvement in sperm motility and an increase in the percentage of sperm with normal morphology. Treatment with DAAs was also associated with increased levels of FT and LH, along with decreased levels of SHBG, prolactin, and E2.

目的:众所周知,丙型肝炎病毒(HCV)感染会影响受感染男性的精液和激素参数。本研究旨在评估使用直接作用抗病毒药物(DAA)清除丙型肝炎病毒对精液和激素参数的影响:方法:共招募了 50 名慢性 HCV 患者,并根据世界卫生组织的指南进行了常规精液分析。在使用DAAs治疗前和治疗3个月后,对总睾酮、游离睾酮(FT)、卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、催乳素和性激素结合球蛋白(SHBG)的基础水平进行了评估:结果:接受 DAA 治疗后,精子活力和 A 级精子比例均有统计学意义的显著提高。此外,畸形精子的比例在治疗后也明显下降(P=0.000)。然而,精液量、浓度和精子总数均无明显差异。DAA治疗后对患者进行的性激素分析显示,FT、LH和FSH水平显著上升,SHBG、催乳素和E2水平显著下降:结论:HCV 清除后,我们注意到精子活力有所改善,形态正常的精子比例有所增加。DAAs治疗也与FT和LH水平升高以及SHBG、催乳素和E2水平降低有关。
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引用次数: 0
Advanced strategies for single embryo selection in assisted human reproduction: A review of clinical practice and research methods. 人类辅助生殖中单胚胎选择的先进策略:临床实践和研究方法综述。
IF 1.8 Q2 Medicine Pub Date : 2024-06-10 DOI: 10.5653/cerm.2023.06478
Zahra Bashiri, Azita Afzali, Morteza Koruji, Hossein Torkashvand, Mehrdad Ghorbanlou, Nadia Sheibak, Zahra Zandieh, Fatemehsadat Amjadi

Among the primary objectives of contemporary assisted reproductive technology research are achieving the births of healthy singletons and improving overall fertility outcomes. Substantial advances have been made in refining the selection of single embryos for transfer, with the aim of maximizing the likelihood of successful implantation. The principal criterion for this selection is embryo morphology. Morphological evaluation systems are based on traditional parameters, including cell count and fragmentation, pronuclear morphology, cleavage rate, blastocyst formation, and various sequential embryonic assessments. To reduce the incidence of multiple pregnancies and to identify the single embryo with the highest potential for growth, invasive techniques such as preimplantation genetic screening are employed in in vitro fertilization clinics. However, new approaches have been suggested for clinical application that do not harm the embryo and that provide consistent, accurate results. Noninvasive technologies, such as time-lapse imaging and omics, leverage morphokinetic parameters and the byproducts of embryo metabolism, respectively, to identify noninvasive prognostic markers for competent single embryo selection. While these technologies have garnered considerable interest in the research community, they are not incorporated into routine clinical practice and still have substantial room for improvement. Currently, the most promising strategies involve integrating multiple methodologies, which together are anticipated to increase the likelihood of successful pregnancy.

当代辅助生殖技术研究的主要目标之一是实现健康单胎的出生和改善整体生育结果。为了最大限度地提高成功植入的可能性,在改进单个胚胎移植的选择方面取得了长足的进步。选择的主要标准是胚胎形态。形态学评估系统以传统参数为基础,包括细胞计数和分裂、前核形态、裂解率、囊胚形成和各种胚胎序列评估。为了降低多胎妊娠的发生率,并确定最具生长潜力的单个胚胎,体外受精诊所采用了植入前遗传学筛查等侵入性技术。然而,临床应用中出现了一些新方法,它们不会伤害胚胎,而且能提供一致、准确的结果。无创技术,如延时成像和全息技术,分别利用形态动力学参数和胚胎新陈代谢的副产物来确定无创预后标记,以进行合格的单胚胎筛选。虽然这些技术已引起研究界的极大兴趣,但它们并未被纳入常规临床实践,仍有很大的改进空间。目前,最有前途的策略是整合多种方法,预计这些方法将共同提高成功妊娠的可能性。
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引用次数: 0
In vitro fertilization as an option for couples with genetic disorders. 将体外受精作为患有遗传性疾病的夫妇的一种选择。
IF 1.8 Q2 Medicine Pub Date : 2024-06-10 DOI: 10.5653/cerm.2023.06667
F Yudha Christianti, Legiran Legiran

Errors in human DNA may cause genetic disorders. Technological developments have raised hopes for reducing the risks of genetic inheritance among married couples who have a history of such disorders. Among the developments in reproductive health technology that reduce those risks is the in vitro fertilization (IVF) process. This review aimed to describe the current strategies using IVF and preimplantation genetic testing (PGT), which would be effective for couples with genetic disorders to have healthy offspring. The literature review included full-text, open-access research articles from ScienceDirect, PubMed, and Google Scholar that were published between 2013 and 2023, with 65 articles obtained from various journals. The keywords were "in vitro fertilization," "reproductive genetic disorders," "PGT-A," "PGT-M," "PGT-SR," and "oocyte donor." A total of 46 articles were selected as the most relevant to the review topic, and the results show that the IVF process can be an option for couples with a history of genetic disorders. Several additional procedures can be performed following IVF, such as oocyte donation and PGT, to help couples who want to have offspring without transmitting their genetic disorders. IVF can be an option for couples who have or carry genetic disorders. With IVF, couples can undertake several procedures such as oocyte donation and PGT for aneuploidy, monogenic disorders, or structural rearrangement.

人类 DNA 中的错误可能导致遗传疾病。技术的发展为降低有遗传病史的已婚夫妇的遗传风险带来了希望。在生殖健康技术的发展中,体外受精(IVF)过程可以降低这些风险。本综述旨在描述目前使用体外受精和植入前基因检测(PGT)的策略,这些策略将有效帮助有遗传疾病的夫妇生育健康的后代。文献综述包括ScienceDirect、PubMed和Google Scholar上发表于2013年至2023年间的全文开放存取研究文章,其中65篇文章来自不同期刊。关键词为 "体外受精"、"生殖遗传疾病"、"PGT-A"、"PGT-M"、"PGT-SR "和 "卵细胞捐赠者"。结果显示,试管婴儿过程可以成为有遗传病史的夫妇的一种选择。体外受精后还可以进行一些额外的程序,如卵细胞捐赠和PGT,以帮助那些希望在不遗传其遗传疾病的情况下生育后代的夫妇。对于患有或携带遗传疾病的夫妇来说,体外受精也是一种选择。通过体外受精,夫妇可以进行多项程序,如卵细胞捐献和非整倍体、单基因遗传病或结构重排的 PGT。
{"title":"In vitro fertilization as an option for couples with genetic disorders.","authors":"F Yudha Christianti, Legiran Legiran","doi":"10.5653/cerm.2023.06667","DOIUrl":"https://doi.org/10.5653/cerm.2023.06667","url":null,"abstract":"<p><p>Errors in human DNA may cause genetic disorders. Technological developments have raised hopes for reducing the risks of genetic inheritance among married couples who have a history of such disorders. Among the developments in reproductive health technology that reduce those risks is the in vitro fertilization (IVF) process. This review aimed to describe the current strategies using IVF and preimplantation genetic testing (PGT), which would be effective for couples with genetic disorders to have healthy offspring. The literature review included full-text, open-access research articles from ScienceDirect, PubMed, and Google Scholar that were published between 2013 and 2023, with 65 articles obtained from various journals. The keywords were \"in vitro fertilization,\" \"reproductive genetic disorders,\" \"PGT-A,\" \"PGT-M,\" \"PGT-SR,\" and \"oocyte donor.\" A total of 46 articles were selected as the most relevant to the review topic, and the results show that the IVF process can be an option for couples with a history of genetic disorders. Several additional procedures can be performed following IVF, such as oocyte donation and PGT, to help couples who want to have offspring without transmitting their genetic disorders. IVF can be an option for couples who have or carry genetic disorders. With IVF, couples can undertake several procedures such as oocyte donation and PGT for aneuploidy, monogenic disorders, or structural rearrangement.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The lncRNA Gm8097 is associated with hypospermatogenesis. lncRNA Gm8097 与精子发生功能低下有关。
IF 1.8 Q2 Medicine Pub Date : 2024-06-10 DOI: 10.5653/cerm.2024.06835
Bin Lei, Luwei Ye, Zhuolin Qiu, Shoubo Zhang

Objective: To investigate whether long non-coding RNA (lncRNA) Gm8097 (LncGm8097) is associated with male infertility.

Methods: The expression and bilogical role of LncGm8097 were investigated.

Results: LncGm8097 expression was down-regulated in the testis tissues with moderate and severe hypospermatogenesis compared with those with normal spermatogenesis and mild hypospermatogenesis (p<0.05). LncGm8097 down-regulation significantly promoted apoptosis and inhibited proliferation in GC1 and GC2 cells. In addition, LncGm8097 was significantly down-regulated in mouse model of hypospermatogenesis and correlated with cell apoptosis and proliferation. LncGm8097 was located immediately upstream of PRPS2, and correlated with Bcl-2/P53/caspase 6/caspase 9 signal pathway.

Conclusion: LncGm8097 down-regulation correlates with hypospermatogenesis, which may offer new insights into the pathogenesis of male infertility.

目的研究长非编码RNA(lncRNA)Gm8097(LncGm8097)是否与男性不育有关:方法:研究LncGm8097的表达和生物学作用:结果:LncGm8097在中度和重度精子发生功能低下的睾丸组织中表达下调,而在精子发生正常和轻度精子发生功能低下的睾丸组织中表达上调:LncGm8097的下调与精子发生功能减退相关,这可能为男性不育症的发病机制提供了新的见解。
{"title":"The lncRNA Gm8097 is associated with hypospermatogenesis.","authors":"Bin Lei, Luwei Ye, Zhuolin Qiu, Shoubo Zhang","doi":"10.5653/cerm.2024.06835","DOIUrl":"https://doi.org/10.5653/cerm.2024.06835","url":null,"abstract":"<p><strong>Objective: </strong>To investigate whether long non-coding RNA (lncRNA) Gm8097 (LncGm8097) is associated with male infertility.</p><p><strong>Methods: </strong>The expression and bilogical role of LncGm8097 were investigated.</p><p><strong>Results: </strong>LncGm8097 expression was down-regulated in the testis tissues with moderate and severe hypospermatogenesis compared with those with normal spermatogenesis and mild hypospermatogenesis (p<0.05). LncGm8097 down-regulation significantly promoted apoptosis and inhibited proliferation in GC1 and GC2 cells. In addition, LncGm8097 was significantly down-regulated in mouse model of hypospermatogenesis and correlated with cell apoptosis and proliferation. LncGm8097 was located immediately upstream of PRPS2, and correlated with Bcl-2/P53/caspase 6/caspase 9 signal pathway.</p><p><strong>Conclusion: </strong>LncGm8097 down-regulation correlates with hypospermatogenesis, which may offer new insights into the pathogenesis of male infertility.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monthly variations in semen parameters in a Sri Lankan population undergoing fertility evaluation. 每月精液参数的变化在斯里兰卡人口进行生育评估。
IF 1.8 Q2 Medicine Pub Date : 2024-06-01 Epub Date: 2023-12-01 DOI: 10.5653/cerm.2023.06240
Anura Dissanayake

Objective: Seasonal variations in semen quality are known to occur in temperate regions, but results regarding tropical areas remain inconclusive. The aim of this study was to determine whether monthly variations in semen parameters are present among men in a tropical region.

Methods: Data were retrospectively collected from semen analyses of 3,000 men over a 10-year period, from 2012 to 2022. Analysis of variance and the independent-samples t-test were employed to observe variations in semen parameters throughout the entire period and between months, respectively.

Results: The mean±standard deviation sperm concentration was significantly lower in June, at 42.5±31.4 million/mL, compared to other months. The highest sperm concentration was found in March, at 57.8±42.6 million/mL, constituting a mean difference of 15.3 million/mL between the lowest and highest concentrations. The total sperm count displayed a similar pattern of monthly variation, with a difference of 47.2 million between the highest and lowest months. No significant monthly differences were observed in other parameters, such as sperm motility, morphology, and semen volume.

Conclusion: Significant monthly variations in sperm concentration and total sperm count were evident in this Sri Lankan population. March, which displayed the highest sperm counts, is in the spring in temperate regions, while the month with the lowest counts, July, is part of the summer. Fluctuations in photoperiod appear to most strongly influence these variations.

目的:精液质量的季节性变化已知发生在温带地区,但关于热带地区的结果仍不确定。本研究的目的是确定在热带地区的男性中是否存在精液参数的月度变化。方法:回顾性收集了2012年至2022年10年间3000名男性的精液分析数据。采用方差分析和独立样本t检验分别观察全期和月间精液参数的变化。结果:6月份精子浓度的平均值±标准差明显低于其他月份,为42.5±3140万/mL。精子浓度最高的月份是3月份,为57.8±4260万/mL,最低和最高浓度的平均差值为1530万/mL。精子总数显示出类似的月度变化模式,最高和最低月份之间的差异为4720万。其他参数,如精子活力、形态和精液量,没有观察到明显的月度差异。结论:在这个斯里兰卡人群中,精子浓度和精子总数的月度变化是明显的。在温带地区,精子数量最多的3月份正值春季,而精子数量最少的7月份正值夏季。光周期的波动似乎对这些变化影响最大。
{"title":"Monthly variations in semen parameters in a Sri Lankan population undergoing fertility evaluation.","authors":"Anura Dissanayake","doi":"10.5653/cerm.2023.06240","DOIUrl":"10.5653/cerm.2023.06240","url":null,"abstract":"<p><strong>Objective: </strong>Seasonal variations in semen quality are known to occur in temperate regions, but results regarding tropical areas remain inconclusive. The aim of this study was to determine whether monthly variations in semen parameters are present among men in a tropical region.</p><p><strong>Methods: </strong>Data were retrospectively collected from semen analyses of 3,000 men over a 10-year period, from 2012 to 2022. Analysis of variance and the independent-samples t-test were employed to observe variations in semen parameters throughout the entire period and between months, respectively.</p><p><strong>Results: </strong>The mean±standard deviation sperm concentration was significantly lower in June, at 42.5±31.4 million/mL, compared to other months. The highest sperm concentration was found in March, at 57.8±42.6 million/mL, constituting a mean difference of 15.3 million/mL between the lowest and highest concentrations. The total sperm count displayed a similar pattern of monthly variation, with a difference of 47.2 million between the highest and lowest months. No significant monthly differences were observed in other parameters, such as sperm motility, morphology, and semen volume.</p><p><strong>Conclusion: </strong>Significant monthly variations in sperm concentration and total sperm count were evident in this Sri Lankan population. March, which displayed the highest sperm counts, is in the spring in temperate regions, while the month with the lowest counts, July, is part of the summer. Fluctuations in photoperiod appear to most strongly influence these variations.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11140258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138463433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence and confounders of chronic endometritis diagnosed using CD138 in patients with recurrent implantation failure. 复发性着床失败患者中使用 CD138 诊断出的慢性子宫内膜炎的发病率和混杂因素。
IF 1.8 Q2 Medicine Pub Date : 2024-06-01 Epub Date: 2024-01-24 DOI: 10.5653/cerm.2023.06170
Hye Jeong Hue, Hyunji Choi, Hyun Kyoung Lee, Jung Ryeol Lee, Byung Chul Jee, Chang Woo Choo, Seul Ki Kim

Objective: This retrospective study aimed to investigate the prevalence of chronic endometritis, diagnosed using CD138 immunohistochemistry, among infertile women and to assess the association between chronic endometritis and recurrent implantation failure (RIF).

Methods: In total, 266 patients who underwent hysteroscopy due to infertility between 2019 and 2020 were included in the analysis. Of these, 136 patients with RIF and 130 non-RIF patients were included in the study. CD138 immunohistochemistry test results, blood biomarkers (including natural killer cells, white blood cells, and the lymphocyte-to-neutrophil ratio), and data on pregnancy outcomes were obtained. If the CD138 test yielded a positive result, the patients received antibiotic treatment.

Results: The overall proportion of CD138-positive patients was 32.7% (87/266). The CD138 positivity rate was not related to the number of cycles with implantation failure. In the RIF patient group, no significant associations were found between CD138 positivity and peripheral blood markers. The clinical pregnancy rates were similar between infertile women treated with antibiotics for chronic endometritis and those without chronic endometritis.

Conclusion: To improve the pregnancy rate in infertile patients, it may be helpful to combine CD138 testing with other laboratory tests and administer antibiotic treatment if the result is positive.

研究目的这项回顾性研究旨在调查不孕妇女中使用 CD138 免疫组化诊断的慢性子宫内膜炎的患病率,并评估慢性子宫内膜炎与复发性着床失败(RIF)之间的关联:共有 266 名在 2019 年至 2020 年期间因不孕而接受宫腔镜检查的患者被纳入分析。其中,136 名 RIF 患者和 130 名非 RIF 患者被纳入研究。研究人员获得了 CD138 免疫组化检测结果、血液生物标志物(包括自然杀伤细胞、白细胞和淋巴细胞与中性粒细胞比率)以及妊娠结局数据。如果 CD138 检测结果呈阳性,患者将接受抗生素治疗:CD138阳性患者的总比例为32.7%(87/266)。CD138 阳性率与植入失败的周期数无关。在 RIF 患者组中,CD138 阳性与外周血标志物之间没有发现明显的关联。结论:使用抗生素治疗慢性子宫内膜炎的不孕妇女与无慢性子宫内膜炎的不孕妇女的临床妊娠率相似:结论:为提高不孕症患者的妊娠率,将 CD138 检测与其他实验室检测结合起来并在检测结果呈阳性时进行抗生素治疗可能会有所帮助。
{"title":"Prevalence and confounders of chronic endometritis diagnosed using CD138 in patients with recurrent implantation failure.","authors":"Hye Jeong Hue, Hyunji Choi, Hyun Kyoung Lee, Jung Ryeol Lee, Byung Chul Jee, Chang Woo Choo, Seul Ki Kim","doi":"10.5653/cerm.2023.06170","DOIUrl":"10.5653/cerm.2023.06170","url":null,"abstract":"<p><strong>Objective: </strong>This retrospective study aimed to investigate the prevalence of chronic endometritis, diagnosed using CD138 immunohistochemistry, among infertile women and to assess the association between chronic endometritis and recurrent implantation failure (RIF).</p><p><strong>Methods: </strong>In total, 266 patients who underwent hysteroscopy due to infertility between 2019 and 2020 were included in the analysis. Of these, 136 patients with RIF and 130 non-RIF patients were included in the study. CD138 immunohistochemistry test results, blood biomarkers (including natural killer cells, white blood cells, and the lymphocyte-to-neutrophil ratio), and data on pregnancy outcomes were obtained. If the CD138 test yielded a positive result, the patients received antibiotic treatment.</p><p><strong>Results: </strong>The overall proportion of CD138-positive patients was 32.7% (87/266). The CD138 positivity rate was not related to the number of cycles with implantation failure. In the RIF patient group, no significant associations were found between CD138 positivity and peripheral blood markers. The clinical pregnancy rates were similar between infertile women treated with antibiotics for chronic endometritis and those without chronic endometritis.</p><p><strong>Conclusion: </strong>To improve the pregnancy rate in infertile patients, it may be helpful to combine CD138 testing with other laboratory tests and administer antibiotic treatment if the result is positive.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11140256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139541645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
COVID-19 (SARS-CoV-2) mRNA vaccination does not affect basal sex hormone levels (follicle-stimulating hormone, luteinizing hormone, estradiol) in reproductive-age women. 接种 COVID-19(SARS-CoV-2)mRNA 不会影响育龄妇女的基础性激素水平(卵泡刺激素、黄体生成素、雌二醇)。
IF 1.8 Q2 Medicine Pub Date : 2024-06-01 Epub Date: 2024-03-25 DOI: 10.5653/cerm.2023.06107
Haeng Jun Jeon, Woo Sik Lee, Ji Eun Park, Ji Young Hwang, Ji Won Kim

Objective: People vaccinated with the coronavirus disease 2019 (COVID-19) (severe acute respiratory syndrome coronavirus-2 [SARS-CoV-2]) mRNA vaccine have reported experiencing various adverse effects. For instance, reproductive-age women have presented with complaints of abnormal uterine bleeding or menstrual cycle changes. We speculated that differences in basal sex hormone levels before and after vaccination may be present in women who experienced irregular bleeding or menstrual cycle changes; thus, this study aimed to investigate the differences in basal sex hormone levels of women before and after two doses of SARS-CoV-2 mRNA vaccination.

Methods: This retrospective study included patients who received SARS-CoV-2 mRNA vaccines between January 2021 and February 2022 at a single center. In an outpatient setting, patients were queried regarding their menstrual cycle, the date of SARS-CoV-2 mRNA vaccination, vaccination type, and vaccination side effects. Differences in basal hormone levels (menstrual cycle days 2-3, follicle-stimulating hormone [FSH], luteinizing hormone [LH], and estradiol) before and after vaccination were compared.

Results: Among the 326 patients, patients with no laboratory records of the hormones were excluded. The median time interval between SARS-CoV-2 mRNA vaccination and the laboratory test day was 79 days (interquartile range, 44 to 127). A comparative analysis of these hormones before and after vaccination revealed no significant differences. Subgroup analyses based on age and reported adverse events also found no statistically significant differences.

Conclusion: This study showed no significant differences in basal hormone levels (FSH, LH, and estradiol) before and after SARS-CoV-2 mRNA vaccination.

目的:据报告,接种了冠状病毒病 2019(COVID-19)(严重急性呼吸系统综合征冠状病毒-2 [SARS-CoV-2])mRNA 疫苗的人出现了各种不良反应。例如,育龄妇女抱怨子宫异常出血或月经周期改变。我们推测,出现不规则出血或月经周期变化的女性可能存在接种前后基础性激素水平的差异;因此,本研究旨在调查接种两剂 SARS-CoV-2 mRNA 疫苗前后女性基础性激素水平的差异:这项回顾性研究包括 2021 年 1 月至 2022 年 2 月期间在一个中心接种 SARS-CoV-2 mRNA 疫苗的患者。在门诊环境中,研究人员询问了患者的月经周期、SARS-CoV-2 mRNA 疫苗接种日期、疫苗接种类型以及疫苗接种副作用。比较了接种前后基础激素水平(月经周期第 2-3 天、卵泡刺激素 [FSH]、黄体生成素 [LH] 和雌二醇)的差异:在 326 名患者中,没有激素化验记录的患者被排除在外。接种 SARS-CoV-2 mRNA 疫苗与实验室检测日之间的中位时间间隔为 79 天(四分位距为 44 至 127)。对这些激素在接种疫苗前后的比较分析表明没有明显差异。根据年龄和报告的不良事件进行的分组分析也没有发现统计学上的显著差异:本研究表明,接种 SARS-CoV-2 mRNA 疫苗前后的基础激素水平(FSH、LH 和雌二醇)无明显差异。
{"title":"COVID-19 (SARS-CoV-2) mRNA vaccination does not affect basal sex hormone levels (follicle-stimulating hormone, luteinizing hormone, estradiol) in reproductive-age women.","authors":"Haeng Jun Jeon, Woo Sik Lee, Ji Eun Park, Ji Young Hwang, Ji Won Kim","doi":"10.5653/cerm.2023.06107","DOIUrl":"10.5653/cerm.2023.06107","url":null,"abstract":"<p><strong>Objective: </strong>People vaccinated with the coronavirus disease 2019 (COVID-19) (severe acute respiratory syndrome coronavirus-2 [SARS-CoV-2]) mRNA vaccine have reported experiencing various adverse effects. For instance, reproductive-age women have presented with complaints of abnormal uterine bleeding or menstrual cycle changes. We speculated that differences in basal sex hormone levels before and after vaccination may be present in women who experienced irregular bleeding or menstrual cycle changes; thus, this study aimed to investigate the differences in basal sex hormone levels of women before and after two doses of SARS-CoV-2 mRNA vaccination.</p><p><strong>Methods: </strong>This retrospective study included patients who received SARS-CoV-2 mRNA vaccines between January 2021 and February 2022 at a single center. In an outpatient setting, patients were queried regarding their menstrual cycle, the date of SARS-CoV-2 mRNA vaccination, vaccination type, and vaccination side effects. Differences in basal hormone levels (menstrual cycle days 2-3, follicle-stimulating hormone [FSH], luteinizing hormone [LH], and estradiol) before and after vaccination were compared.</p><p><strong>Results: </strong>Among the 326 patients, patients with no laboratory records of the hormones were excluded. The median time interval between SARS-CoV-2 mRNA vaccination and the laboratory test day was 79 days (interquartile range, 44 to 127). A comparative analysis of these hormones before and after vaccination revealed no significant differences. Subgroup analyses based on age and reported adverse events also found no statistically significant differences.</p><p><strong>Conclusion: </strong>This study showed no significant differences in basal hormone levels (FSH, LH, and estradiol) before and after SARS-CoV-2 mRNA vaccination.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11140255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beneficial effects of oral antioxidant supplementation on semen quality parameters, reproductive hormones, and sperm DNA integrity in men with idiopathic oligoasthenoteratozoospermia. 口服抗氧化剂对特发性少精症男性精液质量指标、生殖激素和精子DNA完整性的有益影响
IF 1.8 Q2 Medicine Pub Date : 2024-06-01 Epub Date: 2024-03-25 DOI: 10.5653/cerm.2023.06555
Chaymae Rochdi, Meriem Ouadrhiri, Larbi Allai, Ibtissam Bellajdel, Samira Mamri, Hafsa Taheri, Hanane Saadi, Ahmed Mimouni, Mohammed Choukri

Objective: Recently, oral antioxidants in combined forms have been used to treat men with idiopathic infertility. This study aimed to evaluate the effects of treatment with vitamin C, vitamin E, selenium, zinc, arginine, L-carnitine, and coenzyme Q10 on sperm quality parameters, DNA integrity, reproductive hormones, and pregnancy rates in men with infertility and idiopathic oligoasthenoteratozoospermia (OAT).

Methods: A prospective study was conducted on 420 men with infertility and idiopathic OAT who took an oral supplement of antioxidant SP-Power tablets twice daily for 6 months. Semen quality, reproductive hormones, and the DNA fragmentation index (DFI) were evaluated at baseline and at 3 and 6 months after supplementation, using the World Health Organization 2021 guidelines.

Results: No significant difference was observed in volume or the percentage of typical morphology during treatment. A significant improvement in sperm concentration was observed after supplementation (8.67±1.41, 12.17±1.91, and 19.01±0.86 at baseline, 3, and 6 months respectively, p<0.01). The total motility, progressive motility, and total motile sperm count also increased significantly (p<0.01), whereas the DFI decreased after 6 months. There was an increase in normal FSH levels and testosterone levels after 6 months of supplementation of antioxidant SP-Power but these differences were not statistically significant (p=not significant and p=0.06, respectively).

Conclusion: Supplementation with SP-Power tablets improved sperm quality parameters, sperm DFI, some reproductive hormones, and pregnancy rates in men with infertility and idiopathic OAT, which could be attributed to the supplement's synergistic antioxidant action. Further studies are needed to determine the effects of supplementation on oxidative stress markers.

目的:最近,口服联合抗氧化剂被用于治疗男性特发性不育症。本研究旨在评估维生素 C、维生素 E、硒、锌、精氨酸、左旋肉碱和辅酶 Q10 对男性不育症和特发性少精症(OAT)患者的精子质量指标、DNA 完整性、生殖激素和妊娠率的影响:对420名患有不育症和特发性少精子症的男性进行了一项前瞻性研究,这些男性每天口服两次抗氧化剂SP-Power片剂,为期6个月。根据世界卫生组织2021年指南,在补充剂服用后的基线期、3个月和6个月对精液质量、生殖激素和DNA碎片指数(DFI)进行了评估:结果:在治疗过程中,精子数量和典型形态的比例均无明显差异。结果表明:治疗期间,精子数量和典型形态百分比无明显差异;补充 SP-Power 片剂后,精子浓度有明显改善(基线、3 个月和 6 个月时分别为 8.67±1.41、12.17±1.91 和 19.01±0.86,p):补充 SP-Power 片剂可改善不育症和特发性 OAT 男性患者的精子质量参数、精子 DFI、某些生殖激素和受孕率,这可能归功于补充剂的协同抗氧化作用。要确定补充剂对氧化应激指标的影响,还需要进一步的研究。
{"title":"Beneficial effects of oral antioxidant supplementation on semen quality parameters, reproductive hormones, and sperm DNA integrity in men with idiopathic oligoasthenoteratozoospermia.","authors":"Chaymae Rochdi, Meriem Ouadrhiri, Larbi Allai, Ibtissam Bellajdel, Samira Mamri, Hafsa Taheri, Hanane Saadi, Ahmed Mimouni, Mohammed Choukri","doi":"10.5653/cerm.2023.06555","DOIUrl":"10.5653/cerm.2023.06555","url":null,"abstract":"<p><strong>Objective: </strong>Recently, oral antioxidants in combined forms have been used to treat men with idiopathic infertility. This study aimed to evaluate the effects of treatment with vitamin C, vitamin E, selenium, zinc, arginine, L-carnitine, and coenzyme Q10 on sperm quality parameters, DNA integrity, reproductive hormones, and pregnancy rates in men with infertility and idiopathic oligoasthenoteratozoospermia (OAT).</p><p><strong>Methods: </strong>A prospective study was conducted on 420 men with infertility and idiopathic OAT who took an oral supplement of antioxidant SP-Power tablets twice daily for 6 months. Semen quality, reproductive hormones, and the DNA fragmentation index (DFI) were evaluated at baseline and at 3 and 6 months after supplementation, using the World Health Organization 2021 guidelines.</p><p><strong>Results: </strong>No significant difference was observed in volume or the percentage of typical morphology during treatment. A significant improvement in sperm concentration was observed after supplementation (8.67±1.41, 12.17±1.91, and 19.01±0.86 at baseline, 3, and 6 months respectively, p<0.01). The total motility, progressive motility, and total motile sperm count also increased significantly (p<0.01), whereas the DFI decreased after 6 months. There was an increase in normal FSH levels and testosterone levels after 6 months of supplementation of antioxidant SP-Power but these differences were not statistically significant (p=not significant and p=0.06, respectively).</p><p><strong>Conclusion: </strong>Supplementation with SP-Power tablets improved sperm quality parameters, sperm DFI, some reproductive hormones, and pregnancy rates in men with infertility and idiopathic OAT, which could be attributed to the supplement's synergistic antioxidant action. Further studies are needed to determine the effects of supplementation on oxidative stress markers.</p>","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11140257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140207858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Clinical and Experimental Reproductive Medicine-CERM
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