Pub Date : 2024-11-05DOI: 10.1016/j.lanmic.2024.100964
Maria Cambra-Pellejà, Lisette van Lieshout, Luis Baptista-Pires, Miguel Vilaplana, José Muñoz, Javier Gandasegui, Claudio Parolo
Helminthiases are highly prevalent but neglected infections that affect more than 1·5 billion people worldwide. Considering the worldwide prevalence of helminthiases, WHO has declared them a public health concern since 2001, necessitating rigorous control and elimination efforts. However, only a few reliable point-of-care diagnostic tests are available for assessing the effectiveness of public health interventions targeting helminthiases, thus increasing the risk of suboptimal outcomes, misallocation of resources, and emergence of drug-resistant helminths. This Review provides an introduction on helminthiases and strategies to achieve control, elimination, interruption in transmission, and eradication of these infections. The Review then comprehensively details the existent biosensors that can be used to detect these infections in human samples, focusing on their target biomarkers, the bioreceptors used, and the sensing readouts. The Review concludes with an in-depth discussion on the persistent challenges related to helminthiases, aiming to encourage the development of much-needed diagnostics specific to these neglected infections.
{"title":"Crucial role of biosensors in the detection of helminth biomarkers in public health programmes.","authors":"Maria Cambra-Pellejà, Lisette van Lieshout, Luis Baptista-Pires, Miguel Vilaplana, José Muñoz, Javier Gandasegui, Claudio Parolo","doi":"10.1016/j.lanmic.2024.100964","DOIUrl":"https://doi.org/10.1016/j.lanmic.2024.100964","url":null,"abstract":"<p><p>Helminthiases are highly prevalent but neglected infections that affect more than 1·5 billion people worldwide. Considering the worldwide prevalence of helminthiases, WHO has declared them a public health concern since 2001, necessitating rigorous control and elimination efforts. However, only a few reliable point-of-care diagnostic tests are available for assessing the effectiveness of public health interventions targeting helminthiases, thus increasing the risk of suboptimal outcomes, misallocation of resources, and emergence of drug-resistant helminths. This Review provides an introduction on helminthiases and strategies to achieve control, elimination, interruption in transmission, and eradication of these infections. The Review then comprehensively details the existent biosensors that can be used to detect these infections in human samples, focusing on their target biomarkers, the bioreceptors used, and the sensing readouts. The Review concludes with an in-depth discussion on the persistent challenges related to helminthiases, aiming to encourage the development of much-needed diagnostics specific to these neglected infections.</p>","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":" ","pages":"100964"},"PeriodicalIF":20.9,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-05DOI: 10.1016/j.lanmic.2024.101023
Ziyi Wang, Lei Zhang, Jun Li, Yaling Li
{"title":"New developments in hepatitis C virus research in Benin for its elimination.","authors":"Ziyi Wang, Lei Zhang, Jun Li, Yaling Li","doi":"10.1016/j.lanmic.2024.101023","DOIUrl":"https://doi.org/10.1016/j.lanmic.2024.101023","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":" ","pages":"101023"},"PeriodicalIF":20.9,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survey and control of antimicrobial resistance: need for pragmatic options in low-resource settings.","authors":"Selam Bogale Gissa, Soliyana Dejene Zewdie, Ayelign Derebe Kindie, Behailu Tsegaye Mugoro, Thor-Henrik Henriksen","doi":"10.1016/j.lanmic.2024.101021","DOIUrl":"https://doi.org/10.1016/j.lanmic.2024.101021","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":" ","pages":"101021"},"PeriodicalIF":20.9,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-05DOI: 10.1016/j.lanmic.2024.101017
Ting-Yu Yeh, Patrick J Feehley, Michael C Feehley, Chieh-Fan Chen, Tung-Yuan Tsai, Hsiang-Lan Cheng, Gregory P Contreras
{"title":"Rapidly evolving recombinant monkeypox virus strains compound the challenges of the 2024 mpox outbreak.","authors":"Ting-Yu Yeh, Patrick J Feehley, Michael C Feehley, Chieh-Fan Chen, Tung-Yuan Tsai, Hsiang-Lan Cheng, Gregory P Contreras","doi":"10.1016/j.lanmic.2024.101017","DOIUrl":"https://doi.org/10.1016/j.lanmic.2024.101017","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":" ","pages":"101017"},"PeriodicalIF":20.9,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142606849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<div><h3>Background</h3><div>Distinguishing <em>Plasmodium falciparum</em> recrudescence from new infections is crucial for the assessment of antimalarial drug efficacy against <em>P falciparum</em>. We aimed to compare the efficacy of different genotyping methods to assess their effect on drug efficacy estimates, particularly in patients from high-transmission settings with polyclonal infections.</div></div><div><h3>Methods</h3><div>In this head-to-head comparison study, we compared five different genotyping methods currently used: fast capillary electrophoresis (F-CE) using <em>msp1, msp2,</em> and <em>glurp</em>; high-resolution capillary electrophoresis (H-CE) using <em>msp1, msp2,</em> and <em>glurp</em>; H-CE using microsatellites; targeted amplicon deep sequencing (TADS) using single nucleotide polymorphism (SNP)-rich markers; and high-resolution melting (HRM) analysis using <em>msp1</em> and <em>msp2</em>. We assessed their sensitivity in detecting minority clones in polyclonal infections, their reproducibility, and the genetic diversity of the markers used. Our study used four well characterised <em>P falciparum</em> laboratory strains mixed in varying ratios, and 20 paired samples collected from an in-vivo clinical trial. The experiments were performed at the Swiss Tropical and Public Health Institute in Basel, Switzerland between May 5, 2020, and Aug 23, 2021.</div></div><div><h3>Findings</h3><div>H-CE using <em>msp1</em> and <em>msp2</em> and TADS revealed the highest sensitivity in detecting minority clones (up to ratios of 1:100 for H-CE and 50:1:1:1 for TADS in the FCB1:HB3 and 3D7:K1:HB3:FCB1 laboratory strain mixtures, respectively), highest reproducibility (intra-assay: 99% and 91% for H-CE and TADS, respectively; inter-assay: 98% and 92% for H-CE and TADS, respectively), and highest genetic diversity in the used markers (up to 36 and 32 unique genotypes in 20 paired samples for H-CE using <em>msp2</em> and TADS using <em>cpmp,</em> respectively). Microsatellites assessed by H-CE had a lower genetic diversity compared with <em>msp1, msp2,</em> and <em>glurp</em> assessed by H-CE and the SNP-rich markers assessed by TADS, with a maximum of 13 unique genotypes, and some genotypes having allelic frequencies larger than 30%. Markers used by TADS gave the most consistent results in distinguishing recrudescence from new infection across all methods (in 18 of 20 pairs of samples <em>vs</em> 15 of 20 pairs for H-CE).</div></div><div><h3>Interpretation</h3><div>WHO currently recommends replacing <em>glurp</em> with microsatellites. However, in this study, the replacement of <em>glurp</em> with microsatellites did not change the genotyping outcome, probably due to the lower genetic diversity of microsatellites. More studies with large sample sizes are required to identify the most suitable microsatellites that could replace <em>glurp</em>. Our study indicates that TADS should be considered the gold standard for genotyping to distinguish r
{"title":"Genotyping methods to distinguish Plasmodium falciparum recrudescence from new infection for the assessment of antimalarial drug efficacy: an observational, single-centre, comparison study","authors":"Annina Schnoz MSc , Carla Beuret MSc , Maura Concu MSc , Salome Hosch PhD , Liliana K Rutaihwa PhD , Monica Golumbeanu PhD , Christian Nsanzabana PhD","doi":"10.1016/S2666-5247(24)00153-8","DOIUrl":"10.1016/S2666-5247(24)00153-8","url":null,"abstract":"<div><h3>Background</h3><div>Distinguishing <em>Plasmodium falciparum</em> recrudescence from new infections is crucial for the assessment of antimalarial drug efficacy against <em>P falciparum</em>. We aimed to compare the efficacy of different genotyping methods to assess their effect on drug efficacy estimates, particularly in patients from high-transmission settings with polyclonal infections.</div></div><div><h3>Methods</h3><div>In this head-to-head comparison study, we compared five different genotyping methods currently used: fast capillary electrophoresis (F-CE) using <em>msp1, msp2,</em> and <em>glurp</em>; high-resolution capillary electrophoresis (H-CE) using <em>msp1, msp2,</em> and <em>glurp</em>; H-CE using microsatellites; targeted amplicon deep sequencing (TADS) using single nucleotide polymorphism (SNP)-rich markers; and high-resolution melting (HRM) analysis using <em>msp1</em> and <em>msp2</em>. We assessed their sensitivity in detecting minority clones in polyclonal infections, their reproducibility, and the genetic diversity of the markers used. Our study used four well characterised <em>P falciparum</em> laboratory strains mixed in varying ratios, and 20 paired samples collected from an in-vivo clinical trial. The experiments were performed at the Swiss Tropical and Public Health Institute in Basel, Switzerland between May 5, 2020, and Aug 23, 2021.</div></div><div><h3>Findings</h3><div>H-CE using <em>msp1</em> and <em>msp2</em> and TADS revealed the highest sensitivity in detecting minority clones (up to ratios of 1:100 for H-CE and 50:1:1:1 for TADS in the FCB1:HB3 and 3D7:K1:HB3:FCB1 laboratory strain mixtures, respectively), highest reproducibility (intra-assay: 99% and 91% for H-CE and TADS, respectively; inter-assay: 98% and 92% for H-CE and TADS, respectively), and highest genetic diversity in the used markers (up to 36 and 32 unique genotypes in 20 paired samples for H-CE using <em>msp2</em> and TADS using <em>cpmp,</em> respectively). Microsatellites assessed by H-CE had a lower genetic diversity compared with <em>msp1, msp2,</em> and <em>glurp</em> assessed by H-CE and the SNP-rich markers assessed by TADS, with a maximum of 13 unique genotypes, and some genotypes having allelic frequencies larger than 30%. Markers used by TADS gave the most consistent results in distinguishing recrudescence from new infection across all methods (in 18 of 20 pairs of samples <em>vs</em> 15 of 20 pairs for H-CE).</div></div><div><h3>Interpretation</h3><div>WHO currently recommends replacing <em>glurp</em> with microsatellites. However, in this study, the replacement of <em>glurp</em> with microsatellites did not change the genotyping outcome, probably due to the lower genetic diversity of microsatellites. More studies with large sample sizes are required to identify the most suitable microsatellites that could replace <em>glurp</em>. Our study indicates that TADS should be considered the gold standard for genotyping to distinguish r","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":"5 11","pages":"Article 100914"},"PeriodicalIF":20.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/S2666-5247(24)00174-5
Maartje Visser , Christian J P A Hoebe , Petra F G Wolffs , Janneke C M Heijne
{"title":"Rectal gonorrhoea in women: true infections?—Authors’ reply","authors":"Maartje Visser , Christian J P A Hoebe , Petra F G Wolffs , Janneke C M Heijne","doi":"10.1016/S2666-5247(24)00174-5","DOIUrl":"10.1016/S2666-5247(24)00174-5","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":"5 11","pages":"Article 100932"},"PeriodicalIF":20.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.1016/S2666-5247(24)00134-4
Silvia Bertagnolio MD , Zlatina Dobreva MSc , Chad M Centner MSc , Ioana Diana Olaru PhD , Daniele Donà PhD , Stefano Burzo PhD , Benedikt D Huttner MD , Antoine Chaillon MD , Nebiat Gebreselassie PhD , Teodora Wi MD , Mateusz Hasso-Agopsowicz PhD , Benedetta Allegranzi MD , Hatim Sati MD , Verica Ivanovska PhD , Kavita U Kothari MPH , Hanan H Balkhy MD , Alessandro Cassini MD , Raph L Hamers PhD , Kitty Van Weezenbeek PhD , Igor Rudan
The WHO research agenda for antimicrobial resistance (AMR) in human health has identified 40 research priorities to be addressed by the year 2030. These priorities focus on bacterial and fungal pathogens of crucial importance in addressing AMR, including drug-resistant pathogens causing tuberculosis. These research priorities encompass the entire people-centred journey, covering prevention, diagnosis, and treatment of antimicrobial-resistant infections, in addition to addressing the overarching knowledge gaps in AMR epidemiology, burden and drivers, policies and regulations, and awareness and education. The research priorities were identified through a multistage process, starting with a comprehensive scoping review of knowledge gaps, with expert inputs gathered through a survey and open call. The priority setting involved a rigorous modified Child Health and Nutrition Research Initiative approach, ensuring global representation and applicability of the findings. The ultimate goal of this research agenda is to encourage research and investment in the generation of evidence to better understand AMR dynamics and facilitate policy translation for reducing the burden and consequences of AMR.
世界卫生组织人类健康抗菌药物耐药性（AMR）研究议程确定了到 2030 年要解决的 40 个研究重点。这些优先研究重点关注对解决 AMR 问题至关重要的细菌和真菌病原体，包括导致结核病的耐药病原体。这些研究重点涵盖了以人为本的整个过程，包括抗菌药物耐药性感染的预防、诊断和治疗，以及解决 AMR 流行病学、负担和驱动因素、政策和法规以及认识和教育方面的总体知识差距。研究重点是通过多阶段过程确定的，首先是对知识差距进行全面的范围审查，并通过调查和公开呼吁收集专家意见。在确定优先事项时，采用了经过修改的儿童健康与营养研究计划方法，确保研究结果具有全球代表性和适用性。本研究议程的最终目标是鼓励研究和投资于证据的生成，以更好地了解 AMR 的动态，促进政策转化，减轻 AMR 的负担和后果。
{"title":"WHO global research priorities for antimicrobial resistance in human health","authors":"Silvia Bertagnolio MD , Zlatina Dobreva MSc , Chad M Centner MSc , Ioana Diana Olaru PhD , Daniele Donà PhD , Stefano Burzo PhD , Benedikt D Huttner MD , Antoine Chaillon MD , Nebiat Gebreselassie PhD , Teodora Wi MD , Mateusz Hasso-Agopsowicz PhD , Benedetta Allegranzi MD , Hatim Sati MD , Verica Ivanovska PhD , Kavita U Kothari MPH , Hanan H Balkhy MD , Alessandro Cassini MD , Raph L Hamers PhD , Kitty Van Weezenbeek PhD , Igor Rudan","doi":"10.1016/S2666-5247(24)00134-4","DOIUrl":"10.1016/S2666-5247(24)00134-4","url":null,"abstract":"<div><div>The WHO research agenda for antimicrobial resistance (AMR) in human health has identified 40 research priorities to be addressed by the year 2030. These priorities focus on bacterial and fungal pathogens of crucial importance in addressing AMR, including drug-resistant pathogens causing tuberculosis. These research priorities encompass the entire people-centred journey, covering prevention, diagnosis, and treatment of antimicrobial-resistant infections, in addition to addressing the overarching knowledge gaps in AMR epidemiology, burden and drivers, policies and regulations, and awareness and education. The research priorities were identified through a multistage process, starting with a comprehensive scoping review of knowledge gaps, with expert inputs gathered through a survey and open call. The priority setting involved a rigorous modified Child Health and Nutrition Research Initiative approach, ensuring global representation and applicability of the findings. The ultimate goal of this research agenda is to encourage research and investment in the generation of evidence to better understand AMR dynamics and facilitate policy translation for reducing the burden and consequences of AMR.</div></div>","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":"5 11","pages":"Article 100902"},"PeriodicalIF":20.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11543637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号