Pub Date : 2024-09-04DOI: 10.1016/j.lanmic.2024.07.011
Julie Rodriguez, Zahra Hassani, Carolina Alves Costa Silva, Fay Betsou, Federica Carraturo, Alessio Fasano, Mads Israelsen, Anandhi Iyappan, Aleksander Krag, Amira Metwaly, Robert Schierwagen, Jonel Trebicka, Hub Zwart, Joel Doré, Magali Cordaillat-Simmons, Celine Druart
Although microbiome signatures have been identified in various contexts (ie, pathogenesis of non-communicable diseases and treatment response), qualified microbiome-based biomarkers are currently not in use in clinical practice. The Human Microbiome Action consortium initiated a Delphi survey to establish a consensus on the needs, challenges, and limitations in developing qualified microbiome-based biomarkers. The questionnaire was developed by a scientific committee via literature review and expert interviews. To ensure broad applicability of the results, 307 experts were invited to participate; 114 of them responded to the first round of the survey, 93 of whom completed the second and final round as well. The survey highlighted the experts' confidence in the potential of microbiome-based biomarkers for several indications or pathologies. The paucity of validated analytical methods appears to be the principal factor hindering the qualification of these biomarkers. The survey also showed that clinical implementation of these biomarkers would only be possible if kitted and validated molecular assays with simple interpretation are developed. This initiative serves as a foundation for designing and implementing public-private collaborative projects to overcome the challenges and promote clinical application of microbiome-based biomarkers.
{"title":"State of the art and the future of microbiome-based biomarkers: a multidisciplinary Delphi consensus.","authors":"Julie Rodriguez, Zahra Hassani, Carolina Alves Costa Silva, Fay Betsou, Federica Carraturo, Alessio Fasano, Mads Israelsen, Anandhi Iyappan, Aleksander Krag, Amira Metwaly, Robert Schierwagen, Jonel Trebicka, Hub Zwart, Joel Doré, Magali Cordaillat-Simmons, Celine Druart","doi":"10.1016/j.lanmic.2024.07.011","DOIUrl":"https://doi.org/10.1016/j.lanmic.2024.07.011","url":null,"abstract":"<p><p>Although microbiome signatures have been identified in various contexts (ie, pathogenesis of non-communicable diseases and treatment response), qualified microbiome-based biomarkers are currently not in use in clinical practice. The Human Microbiome Action consortium initiated a Delphi survey to establish a consensus on the needs, challenges, and limitations in developing qualified microbiome-based biomarkers. The questionnaire was developed by a scientific committee via literature review and expert interviews. To ensure broad applicability of the results, 307 experts were invited to participate; 114 of them responded to the first round of the survey, 93 of whom completed the second and final round as well. The survey highlighted the experts' confidence in the potential of microbiome-based biomarkers for several indications or pathologies. The paucity of validated analytical methods appears to be the principal factor hindering the qualification of these biomarkers. The survey also showed that clinical implementation of these biomarkers would only be possible if kitted and validated molecular assays with simple interpretation are developed. This initiative serves as a foundation for designing and implementing public-private collaborative projects to overcome the challenges and promote clinical application of microbiome-based biomarkers.</p>","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":null,"pages":null},"PeriodicalIF":20.9,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-03DOI: 10.1016/j.lanmic.2024.100979
Priya Venkatesan
{"title":"First self-test for hepatitis C virus.","authors":"Priya Venkatesan","doi":"10.1016/j.lanmic.2024.100979","DOIUrl":"https://doi.org/10.1016/j.lanmic.2024.100979","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":null,"pages":null},"PeriodicalIF":20.9,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-03DOI: 10.1016/j.lanmic.2024.100978
Priya Venkatesan
{"title":"Human cases of avian influenza A(H5) in the USA.","authors":"Priya Venkatesan","doi":"10.1016/j.lanmic.2024.100978","DOIUrl":"https://doi.org/10.1016/j.lanmic.2024.100978","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":null,"pages":null},"PeriodicalIF":20.9,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01DOI: 10.1016/S2666-5247(24)00101-0
<div><h3>Background</h3><p>The emerging fungal pathogen <em>Candida auris</em> poses a serious threat to global public health due to its worldwide distribution, multidrug resistance, high transmissibility, propensity to cause outbreaks, and high mortality. We aimed to characterise three unusual <em>C auris</em> isolates detected in Singapore, and to determine whether they constitute a novel clade distinct from all previously known <em>C auris</em> clades (I–V).</p></div><div><h3>Methods</h3><p>In this genotypic and phenotypic study, we characterised three <em>C auris</em> clinical isolates, which were cultured from epidemiologically unlinked inpatients at a large tertiary hospital in Singapore. The index isolate was detected in April, 2023. We performed whole-genome sequencing (WGS) and obtained hybrid assemblies of these <em>C auris</em> isolates. The complete genomes were compared with representative genomes of all known <em>C auris</em> clades. To provide a global context, 3651 international WGS data from the National Center for Biotechnology Information (NCBI) database were included in a high-resolution single nucleotide polymorphism (SNP) analysis. Antifungal susceptibility testing was done and antifungal resistance genes, mating-type locus, and chromosomal rearrangements were characterised from the WGS data of the three investigated isolates. We further implemented Bayesian logistic regression models to classify isolates into known clades and simulate the automatic detection of isolates belonging to novel clades as their WGS data became available.</p></div><div><h3>Findings</h3><p>The three investigated isolates were separated by at least 37 000 SNPs (range 37 000–236 900) from all existing <em>C auris</em> clades. These isolates had opposite mating-type allele and different chromosomal rearrangements when compared with their closest clade IV relatives. The isolates were susceptible to all tested antifungals. Therefore, we propose that these isolates represent a new clade of <em>C auris,</em> clade VI. Furthermore, an independent WGS dataset from Bangladesh, accessed via the NCBI Sequence Read Archive, was found to belong to this new clade. As a proof-of-concept, our Bayesian logistic regression model was able to flag these outlier genomes as a potential new clade.</p></div><div><h3>Interpretation</h3><p>The discovery of a new <em>C auris</em> clade in Singapore and Bangladesh in the Indomalayan zone, showing a close relationship to clade IV members most commonly found in South America, highlights the unknown genetic diversity and origin of <em>C auris</em>, particularly in under-resourced regions. Active surveillance in clinical settings, along with effective sequencing strategies and downstream analysis, will be essential in the identification of novel strains, tracking of transmission, and containment of adverse clinical effects of <em>C auris</em> infections.</p></div><div><h3>Funding</h3><p>Duke-NUS Academic Medical Center Nurturing Cl
背景:新出现的真菌病原体念珠菌对全球公共卫生构成严重威胁,因为它分布于世界各地,具有耐多药、高传播性、易导致疾病爆发和高死亡率等特点。我们的目的是描述在新加坡检测到的三种不同寻常的念珠菌分离株的特征,并确定它们是否构成了一个新的支系,有别于之前已知的所有念珠菌支系(I-V):在这项基因型和表型研究中,我们对从新加坡一家大型三甲医院的无流行病学关联的住院病人身上培养出的三株银环蛇临床分离株进行了鉴定。指数分离株于 2023 年 4 月发现。我们对这些 C auris 分离物进行了全基因组测序(WGS)并获得了杂交组合。我们将完整的基因组与所有已知 C auris 支系的代表性基因组进行了比较。为了提供全球背景,美国国家生物技术信息中心(NCBI)数据库中的 3651 个国际 WGS 数据被纳入了高分辨率单核苷酸多态性(SNP)分析。我们进行了抗真菌药敏试验,并从三个被调查分离物的 WGS 数据中确定了抗真菌药敏基因、交配型基因座和染色体重排的特征。我们进一步采用贝叶斯逻辑回归模型将分离物归入已知支系,并模拟自动检测属于新支系的分离物,因为它们的 WGS 数据已经可用:所调查的三个分离株与所有现有的肛管癣菌支系之间至少有 37 000 个 SNPs(范围为 37 000-236 900)的差异。这些分离株与其最接近的支系 IV 亲属相比,具有相反的交配型等位基因和不同的染色体重排。这些分离物对所有测试过的抗真菌药物都敏感。因此,我们认为这些分离物代表了 C auris 的一个新支系,即支系 VI。此外,通过 NCBI Sequence Read Archive 获取的来自孟加拉国的独立 WGS 数据集也被发现属于这个新支系。作为概念验证,我们的贝叶斯逻辑回归模型能够将这些离群基因组标记为一个潜在的新支系:在新加坡和孟加拉国的印多马克拉亚区发现了一个新的C auris支系,该支系与最常见于南美洲的支系IV成员关系密切,这突显了C auris未知的遗传多样性和起源,尤其是在资源匮乏的地区。在临床环境中积极开展监测,同时采用有效的测序策略和下游分析,对于鉴定新型菌株、追踪传播情况和遏制 C auris 感染的不良临床影响至关重要:杜克大学-新加坡国立大学学术医学中心临床研究人员培养计划(Nurturing Clinician Researcher Scheme)和Genedant-GIS创新计划(Genedant-GIS Innovation Program)。
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Pub Date : 2024-09-01DOI: 10.1016/S2666-5247(24)00107-1
{"title":"To vaccinate or not against highly pathogenic avian influenza?","authors":"","doi":"10.1016/S2666-5247(24)00107-1","DOIUrl":"10.1016/S2666-5247(24)00107-1","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":null,"pages":null},"PeriodicalIF":20.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666524724001071/pdfft?md5=0460eae2fc132680c775001d030173bc&pid=1-s2.0-S2666524724001071-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140960026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01DOI: 10.1016/j.lanmic.2024.100977
{"title":"Candida auris: new clade, same challenges","authors":"","doi":"10.1016/j.lanmic.2024.100977","DOIUrl":"10.1016/j.lanmic.2024.100977","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":null,"pages":null},"PeriodicalIF":20.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666524724002386/pdfft?md5=60b4b15fe34e21b7499564e96d19ae1c&pid=1-s2.0-S2666524724002386-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01DOI: 10.1016/S2666-5247(24)00097-1
{"title":"Harmonising the measurement of neutralising antibodies against chikungunya virus: a path forward for licensing of new vaccines?","authors":"","doi":"10.1016/S2666-5247(24)00097-1","DOIUrl":"10.1016/S2666-5247(24)00097-1","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":null,"pages":null},"PeriodicalIF":20.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666524724000971/pdfft?md5=6c1c52597c47530e515792ccb5212867&pid=1-s2.0-S2666524724000971-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141026114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01DOI: 10.1016/S2666-5247(24)00082-X
<div><h3>Background</h3><p>Although dromedary camels (<em>Camelus dromedarius</em>) are known to be the host reservoir for MERS-CoV, the virus causing Middle East respiratory syndrome (MERS), zoonotic transmission pathways and camel subpopulations posing highest transmission risk are poorly understood. Extensively managed herds, ubiquitous across the Arabian Peninsula, present a major potential source of primary infection. In this study we aimed to address key knowledge gaps regarding MERS epidemiology among high-risk communities associated with such herds, which is essential information for effective control strategies.</p></div><div><h3>Methods</h3><p>We did a cross-sectional study between Sept 27, 2017, and Oct 11, 2018, among members of livestock-owning households in southern Jordan (Aqaba East, Aqaba West, Ma’an East, and Ma’an West regions), with random selection of households (house and tent dwellings) from Ministry of Agriculture lists via computer-generated randomisation lists. Household visits were done, with questionnaires administered to household members regarding potential risk factors for MERS-CoV exposure in the past 6 months and blood samples and nasal and oral swabs collected, alongside physical examination data including blood pressure and blood glucose. Children younger than 5 years and individuals without capacity to provide informed consent were excluded. Serum was tested for IgG antibodies to MERS-CoV spike protein (S1 subunit) and nucleocapsid (N) protein with in-house indirect ELISAs, and viral RNA was detected in nasal and oral samples by RT-PCR. The primary outcome was evidence of MERS-CoV exposure (ascertained by seropositive status on S1 or N ELISAs, or a positive swab sample on RT-PCR); secondary outcomes were potential associations between possible risk factors and seropositive status. RT-PCR data were to be presented descriptively. Seroprevalence estimates were obtained at the individual and household levels, and associations between hypothetical risk factors and seropositive status were assessed with use of mixed-effects logistic regression.</p></div><div><h3>Findings</h3><p>We sampled 879 household members (median age 27 years [IQR 16–44]; 471 [54%] males and 408 [46%] females) from 204 households. 72 (8%) household members were seropositive on S1 ELISA (n=25, 3%) or N ELISA (n=52, 6%). No positive nasal or oral swab samples were identified on RT-PCR. Within-household clustering was identified for seropositivity on S1 ELISA (intraclass correlation coefficient 0·88 [0·35–0·96]) but not N ELISA (0·00 [0·00–0·27]). On multivariable analysis, S1 ELISA seropositivity was associated with frequently (≥weekly) interacting with young (age <1 year) camels (adjusted odds ratio [OR<sub>adj</sub>] 3·85 [95% CI 1·41–11·61], p=0·011), with frequent kissing and petting (OR<sub>adj</sub> 4·56 [1·55–15·42], p=0·0074), and frequent feeding and watering (OR<sub>adj</sub> 4·97 [1·80–15·29], p=0·0027) of young camels identified as
{"title":"MERS-CoV exposure and risk factors for MERS-CoV ELISA seropositivity among members of livestock-owning households in southern Jordan: a population based cross-sectional study","authors":"","doi":"10.1016/S2666-5247(24)00082-X","DOIUrl":"10.1016/S2666-5247(24)00082-X","url":null,"abstract":"<div><h3>Background</h3><p>Although dromedary camels (<em>Camelus dromedarius</em>) are known to be the host reservoir for MERS-CoV, the virus causing Middle East respiratory syndrome (MERS), zoonotic transmission pathways and camel subpopulations posing highest transmission risk are poorly understood. Extensively managed herds, ubiquitous across the Arabian Peninsula, present a major potential source of primary infection. In this study we aimed to address key knowledge gaps regarding MERS epidemiology among high-risk communities associated with such herds, which is essential information for effective control strategies.</p></div><div><h3>Methods</h3><p>We did a cross-sectional study between Sept 27, 2017, and Oct 11, 2018, among members of livestock-owning households in southern Jordan (Aqaba East, Aqaba West, Ma’an East, and Ma’an West regions), with random selection of households (house and tent dwellings) from Ministry of Agriculture lists via computer-generated randomisation lists. Household visits were done, with questionnaires administered to household members regarding potential risk factors for MERS-CoV exposure in the past 6 months and blood samples and nasal and oral swabs collected, alongside physical examination data including blood pressure and blood glucose. Children younger than 5 years and individuals without capacity to provide informed consent were excluded. Serum was tested for IgG antibodies to MERS-CoV spike protein (S1 subunit) and nucleocapsid (N) protein with in-house indirect ELISAs, and viral RNA was detected in nasal and oral samples by RT-PCR. The primary outcome was evidence of MERS-CoV exposure (ascertained by seropositive status on S1 or N ELISAs, or a positive swab sample on RT-PCR); secondary outcomes were potential associations between possible risk factors and seropositive status. RT-PCR data were to be presented descriptively. Seroprevalence estimates were obtained at the individual and household levels, and associations between hypothetical risk factors and seropositive status were assessed with use of mixed-effects logistic regression.</p></div><div><h3>Findings</h3><p>We sampled 879 household members (median age 27 years [IQR 16–44]; 471 [54%] males and 408 [46%] females) from 204 households. 72 (8%) household members were seropositive on S1 ELISA (n=25, 3%) or N ELISA (n=52, 6%). No positive nasal or oral swab samples were identified on RT-PCR. Within-household clustering was identified for seropositivity on S1 ELISA (intraclass correlation coefficient 0·88 [0·35–0·96]) but not N ELISA (0·00 [0·00–0·27]). On multivariable analysis, S1 ELISA seropositivity was associated with frequently (≥weekly) interacting with young (age <1 year) camels (adjusted odds ratio [OR<sub>adj</sub>] 3·85 [95% CI 1·41–11·61], p=0·011), with frequent kissing and petting (OR<sub>adj</sub> 4·56 [1·55–15·42], p=0·0074), and frequent feeding and watering (OR<sub>adj</sub> 4·97 [1·80–15·29], p=0·0027) of young camels identified as ","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":null,"pages":null},"PeriodicalIF":20.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266652472400082X/pdfft?md5=4e23a680a4d80620594bd79b719e967b&pid=1-s2.0-S266652472400082X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141761617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01DOI: 10.1016/S2666-5247(24)00079-X
<div><h3>Background</h3><p>Microbiota alterations are common in patients hospitalised for severe infections, and preclinical models have shown that anaerobic butyrate-producing gut bacteria protect against systemic infections. However, the relationship between microbiota disruptions and increased susceptibility to severe infections in humans remains unclear. We investigated the relationship between gut microbiota and the risk of future infection-related hospitalisation in two large population-based cohorts.</p></div><div><h3>Methods</h3><p>In this observational microbiome study, gut microbiota were characterised using 16S rRNA gene sequencing in independent population-based cohorts from the Netherlands (HELIUS study; derivation cohort) and Finland (FINRISK 2002 study; validation cohort). HELIUS was conducted in Amsterdam, Netherlands, and included adults (aged 18–70 years at inclusion) who were randomly sampled from the municipality register of Amsterdam. FINRISK 2002 was conducted in six regions in Finland and is a population survey that included a random sample of adults (aged 25–74 years). In both cohorts, participants completed questionnaires, underwent a physical examination, and provided a faecal sample at inclusion (Jan 3, 2013, to Nov 27, 2015, for HELIUS participants and Jan 21 to April 19, 2002, for FINRISK participants. For inclusion in our study, a faecal sample needed to be provided and successfully sequenced, and national registry data needed to be available. Primary predictor variables were microbiota composition, diversity, and relative abundance of butyrate-producing bacteria. Our primary outcome was hospitalisation or mortality due to any infectious disease during 5–7-year follow-up after faecal sample collection, based on national registry data. We examined associations between microbiota and infection risk using microbial ecology and Cox proportional hazards.</p></div><div><h3>Findings</h3><p>We profiled gut microbiota from 10 699 participants (4248 [39·7%] from the derivation cohort and 6451 [60·3%] from the validation cohort). 602 (5·6%) participants (152 [3·6%] from the derivation cohort; 450 [7·0%] from the validation cohort) were hospitalised or died due to infections during follow-up. Gut microbiota composition of these participants differed from those without hospitalisation for infections (derivation p=0·041; validation p=0·0002). Specifically, higher relative abundance of butyrate-producing bacteria was associated with a reduced risk of hospitalisation for infections (derivation cohort cause-specific hazard ratio 0·75 [95% CI 0·60–0·94] per 10% increase in butyrate producers, p=0·013; validation cohort 0·86 [0·77–0·96] per 10% increase, p=0·0077). These associations remained unchanged following adjustment for demographics, lifestyle, antibiotic exposure, and comorbidities.</p></div><div><h3>Interpretation</h3><p>Gut microbiota composition, specifically colonisation with butyrate-producing bacteria, was associated with
{"title":"Association between butyrate-producing gut bacteria and the risk of infectious disease hospitalisation: results from two observational, population-based microbiome studies","authors":"","doi":"10.1016/S2666-5247(24)00079-X","DOIUrl":"10.1016/S2666-5247(24)00079-X","url":null,"abstract":"<div><h3>Background</h3><p>Microbiota alterations are common in patients hospitalised for severe infections, and preclinical models have shown that anaerobic butyrate-producing gut bacteria protect against systemic infections. However, the relationship between microbiota disruptions and increased susceptibility to severe infections in humans remains unclear. We investigated the relationship between gut microbiota and the risk of future infection-related hospitalisation in two large population-based cohorts.</p></div><div><h3>Methods</h3><p>In this observational microbiome study, gut microbiota were characterised using 16S rRNA gene sequencing in independent population-based cohorts from the Netherlands (HELIUS study; derivation cohort) and Finland (FINRISK 2002 study; validation cohort). HELIUS was conducted in Amsterdam, Netherlands, and included adults (aged 18–70 years at inclusion) who were randomly sampled from the municipality register of Amsterdam. FINRISK 2002 was conducted in six regions in Finland and is a population survey that included a random sample of adults (aged 25–74 years). In both cohorts, participants completed questionnaires, underwent a physical examination, and provided a faecal sample at inclusion (Jan 3, 2013, to Nov 27, 2015, for HELIUS participants and Jan 21 to April 19, 2002, for FINRISK participants. For inclusion in our study, a faecal sample needed to be provided and successfully sequenced, and national registry data needed to be available. Primary predictor variables were microbiota composition, diversity, and relative abundance of butyrate-producing bacteria. Our primary outcome was hospitalisation or mortality due to any infectious disease during 5–7-year follow-up after faecal sample collection, based on national registry data. We examined associations between microbiota and infection risk using microbial ecology and Cox proportional hazards.</p></div><div><h3>Findings</h3><p>We profiled gut microbiota from 10 699 participants (4248 [39·7%] from the derivation cohort and 6451 [60·3%] from the validation cohort). 602 (5·6%) participants (152 [3·6%] from the derivation cohort; 450 [7·0%] from the validation cohort) were hospitalised or died due to infections during follow-up. Gut microbiota composition of these participants differed from those without hospitalisation for infections (derivation p=0·041; validation p=0·0002). Specifically, higher relative abundance of butyrate-producing bacteria was associated with a reduced risk of hospitalisation for infections (derivation cohort cause-specific hazard ratio 0·75 [95% CI 0·60–0·94] per 10% increase in butyrate producers, p=0·013; validation cohort 0·86 [0·77–0·96] per 10% increase, p=0·0077). These associations remained unchanged following adjustment for demographics, lifestyle, antibiotic exposure, and comorbidities.</p></div><div><h3>Interpretation</h3><p>Gut microbiota composition, specifically colonisation with butyrate-producing bacteria, was associated with","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":null,"pages":null},"PeriodicalIF":20.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266652472400079X/pdfft?md5=fd2df1ffc0f7c117acb299cdf1ceffdd&pid=1-s2.0-S266652472400079X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-01DOI: 10.1016/S2666-5247(24)00086-7
{"title":"Whole-genome sequencing unveils the outbreak of Mycoplasma pneumoniae in mainland China","authors":"","doi":"10.1016/S2666-5247(24)00086-7","DOIUrl":"10.1016/S2666-5247(24)00086-7","url":null,"abstract":"","PeriodicalId":46633,"journal":{"name":"Lancet Microbe","volume":null,"pages":null},"PeriodicalIF":20.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666524724000867/pdfft?md5=8f8393c4d679784a95d437b3aa9237c7&pid=1-s2.0-S2666524724000867-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140767355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}