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The current state of the breeding situation in the herd 畜群中繁殖状况的现状
Pub Date : 2023-01-01 DOI: 10.36871/vet.zoo.bio.202309013
Natalya. I. Abramova, Olga L. Khromova, M. O. Selimyan, Natalia V. Zenkova
The current state of the herd, breeds and prospects for their development should be determined on the basis of studying the variability of the main economically useful signs and actual indicators, which allows us to identify selection and selection techniques that will ensure an increase in the productivity of animals with each new generation. The studies were carried out on a herd of Ayrshire cows on incomplete first lactation, first completed, second, third and older, taking into account the productivity of maternal ancestors. According to the results of the research, a significant variability in the milk yield of cows of the modern herd and their mothers CV=11,9–19,5 % was revealed, which allows for improving the selection of animals for the reproduction of the herd. The most effective selection was established according to the actual indicators of milk yield in mothers at the first lactation +977 kg of milk, at the highest + 456 kg of milk, with a reduction in the age of daughters in lactation. According to MJ and MDB, a weak variability of the CV=3,3–3,5 % trait was revealed in the milk of cows of the modern herd. These research results are a characteristic of the breeding situation in the herd and the possibility of its correction by directional selection and selection of animals.
畜群的现状、品种及其发展前景应在研究主要经济有用标志和实际指标的可变性的基础上确定,这使我们能够确定选择和选择技术,以确保每一代动物的生产力都有所提高。这项研究是在一群未完成第一次哺乳、第一次完成、第二次、第三次和更老的艾尔郡奶牛身上进行的,同时考虑到母系祖先的生产力。根据研究结果,揭示了现代牛群及其母亲的奶牛产奶量CV=11,9 - 19,5%的显着差异,这可以改善牛群繁殖动物的选择。最有效的选择是根据母亲第一次哺乳期产奶量+977公斤,最高产奶量+ 456公斤的实际指标确定的,同时减少了哺乳期女儿的年龄。根据MJ和MDB,在现代牛群的牛奶中显示出CV=3,3 - 3,5 %的弱变异性。这些研究结果是畜群中繁殖状况的一个特征,以及通过定向选择和动物选择对其进行修正的可能性。
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引用次数: 0
Effect of microalgae feeding to lactating cows on milk quality and milk production 饲喂微藻对泌乳奶牛乳品质和产奶量的影响
Pub Date : 2023-01-01 DOI: 10.36871/vet.zoo.bio.202309011
Dmitry V. Bykov, Alexei A. Vasiliev, Maxim V. Syrovatsky
In the conditions of a dairy commercial enterprise, a scientific and economic experiment was carried out on two groups of cows – analogues. The duration of the experiment was 104 days. In a scientific and economic experiment, we studied the effect of feeding a suspension of chlorella in the first phase of lactation in the diets of freshly calved lactating cows and cows 2–3 lactations per amount of milk fat and protein in milk. The analysis of average values according to the dynamics of changes in the content of milk fat was carried out. Data were obtained on the positive effect of chlorella suspension on the quality indicators of fat and protein in the milk of cows of the experimental group.
在某乳业商业企业条件下,对两组奶牛进行了科学经济的试验。试验期104 d。在科学经济试验中,我们研究了在泌乳期第一阶段在新犊牛和每乳中脂肪和蛋白质含量为2-3个泌乳量的奶牛饲粮中添加小球藻悬浮液的效果。根据乳脂含量的动态变化,对平均值进行了分析。获得小球藻悬浮液对试验组奶牛乳中脂肪和蛋白质质量指标的积极影响的数据。
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引用次数: 0
Unification of classification groups of vaccines 统一疫苗分类组
Pub Date : 2023-01-01 DOI: 10.36871/vet.zoo.bio.202309008
Nikolay V. Pimenov, Andrey V. Kapustin, Andrey D. Roenko
Vaccines – immunobiodrugs, for active immunization of people and animals for preventive and therapeutic purposes, today have received a multi-vector and complex development of technology and structure. At the moment, in the world and in the Russian Federation, in particular, there is no single, generally recognized classification of vaccines that combines all modern achievements in vaccinology. The literature uses disparate approaches to classification, naming of vaccines, as well as incomplete reduction of classification groups. In the presented work, an analysis of existing classifications was carried out, their improvement based on new knowledge in the field of vaccine preparations, the creation of new drugs based on genetic engineering, the systematization of scientific information in the field of development of immunobiological preparations and open prototypes. This classification is proposed to be used to create a unified classification of vaccines on the territory of the Russian Federation and the member countries of the Eurasian Economic Union and use it to standardize finished products, educational and methodological publications, scientific reaserches and other theoretical materials. The paper presents four classification groups, each of which is composed in such a way that, if necessary, for example, in the event of the emergence of fundamentally new technologies for the production of vaccines, they can be implemented into it without compromising the overall systematics.
疫苗-免疫生物药物,用于预防和治疗目的的人和动物的主动免疫,今天已经得到了多载体和复杂的技术和结构的发展。目前,在世界上,特别是在俄罗斯联邦,还没有一种统一的、公认的疫苗分类,能够结合疫苗学方面的所有现代成就。文献使用不同的方法来分类,命名疫苗,以及分类组的不完全还原。在目前的工作中,对现有的分类进行了分析,基于疫苗制剂领域的新知识对其进行了改进,基于基因工程的新药创造,在免疫生物学制剂开发领域的科学信息系统化和开放原型。该分类建议用于在俄罗斯联邦和欧亚经济联盟成员国境内建立统一的疫苗分类,并将其用于标准化成品、教育和方法出版物、科学研究和其他理论材料。该文件提出了四个分类组,每一个分类组的组成方式是,如有必要,例如,在出现生产疫苗的根本新技术的情况下,可以在不损害整体系统的情况下将其应用于该分类组。
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引用次数: 0
Study of the effect of mercury and arsenic contamination of feed on the safety of livestock products 饲料中汞和砷污染对畜产品安全影响的研究
Pub Date : 2023-01-01 DOI: 10.36871/vet.zoo.bio.202309012
Irina E. Epifanova, Vadim G. Epimakhov, Vladimir Ya. Sarukhanov
A review of some aspects obtaining environmentally friendly livestock products studies under the conditions of agricultural territories technogenic pollution of for the case of high levels of mercury and arsenic in the agrosphere is presented. The mercury and arsenic toxic effects of on the farm animals physiological state and productivity when taken with the diet, even in small quantities, have been confirmed. The simultaneous analysis of wool, blood and urine animals samples expediency for the presence of mercury and arsenic in order to predict the possible consequences of exposure to these toxicants on the animal organism and determine the environmental safety level of the products obtained is noted. The importance of the public health risk assessing from present in food toxicants impact is emphasized. The conclusion about the predicting the possible mercury and arsenic content of in food raw materials of animal origin problem relevance and the need to ration their intake with the diet in order to obtain animal products that meet sanitary and hygienic requirements is made.
介绍了在农业领域的技术污染条件下,在农业圈中汞和砷含量高的情况下,获得环境友好型牲畜产品研究的一些方面。与饲料一起食用时,即使少量也会对农场动物的生理状态和生产力产生汞和砷的毒性影响,这已得到证实。同时分析动物的羊毛、血液和尿液样本是否存在汞和砷,以便预测接触这些有毒物质对动物机体可能产生的后果,并确定所获得产品的环境安全水平。强调了从食品毒物影响中评估公共健康风险的重要性。对动物源性食品原料中可能存在的汞和砷含量的预测问题的相关性以及为获得符合卫生要求的动物产品而对其摄入量进行定量控制的必要性进行了结论。
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引用次数: 0
Analysis of laboratory diagnostics of chlamydia in animals and birds in the Russian Federation for 2019–2021 2019-2021年俄罗斯联邦动物和鸟类衣原体实验室诊断分析
Pub Date : 2023-01-01 DOI: 10.36871/vet.zoo.bio.202309007
Svetlana V. Zyuzgina, Olga E. Zinovieva, Tatyana P. Lobova, Vera V. Mikhailova, Maria S. Shishkina, Anastasia N. Skvortsova
Chlamydia infection causes significant economic damage to various branches of animal husbandry and poultry farming. In the system of veterinary and sanitary measures, it is important to detect the pathogen as early and reliably as possible in various forms of the disease. For this purpose, a number of laboratory methods of direct and indirect diagnosis of chlamydia have been developed over the past years. The article analyzes the results of laboratory tests for chlamydia in state veterinary laboratories for 2019–2021, as well as cases of positive results in the study of biological and pathological materials from animals, including birds. During the analyzed period, 2 750 726 samples of material from animals, including birds, were received by state veterinary laboratories for research on chlamydia, of which 2 645 605 (96,7 %) samples were examined by serological methods, specific antibodies were detected in 0,4 % of blood serum from animals (except birds). Positive results of serological tests require mandatory confirmation by methods of direct detection of chlamydia, their antigens, nucleic acids in biological material. The genetic material of chlamydia during polymerase chain reaction studies was detected in 1 % of samples of biological and pathological material from animals, including birds.
衣原体感染对畜牧业和家禽养殖业的各个部门造成重大的经济损失。在兽医和卫生措施系统中,在各种形式的疾病中尽早和尽可能可靠地发现病原体是很重要的。为此目的,在过去的几年中已经开发了许多直接和间接诊断衣原体的实验室方法。本文分析了2019-2021年国家兽医实验室对衣原体的实验室检测结果,以及对包括鸟类在内的动物的生物和病理材料进行研究的阳性结果案例。分析期间,国家兽医实验室共收到包括禽类在内的动物标本2750726份,其中血清学检测标本2645605份(96.7%),动物(禽类除外)血清中检测到特异性抗体的比例为0.4%。血清学检测的阳性结果需要通过直接检测衣原体及其抗原和生物材料中的核酸的方法进行强制性确认。在聚合酶链反应研究中,在1%的动物(包括鸟类)生物和病理材料样本中检测到衣原体遗传物质。
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引用次数: 0
General patterns of microscopic structure of horse tendons 马肌腱显微结构的一般模式
Pub Date : 2023-01-01 DOI: 10.36871/vet.zoo.bio.202310002
Elena N. Borkhunova, Mariya V. Zhukova
The work analyzed the results of the study of the tendons of the finger of a horse and presented general patterns of their structural organization. It was shown that the dense decorated connective tissue of the tendon, in the structure of which there are cells (tenoblasts and tenocytes) and intercellular matter with a predominance of bundles of collagen fibers, has a bundle structure, is in close connection with the loose connective tissue that forms endo- and peritenone. In turn, together these tissues form functional units in the tendon structure, which form the internal structure of the tendon as an organ. At the level of functional units, tendon tissue is remodeled. According to our data, the most labile link that allows the tendon to realize structural and metabolic adaptation to biomechanical load may be bundles of second-order collagen fibers, which differ in the variability of morphological (cross-sectional shape) and morphometric (cross-sectional area) indicators. Tendon tissue adapts to different mechanical loads by changing the structure that can be quantified. Thus, it varies the cross-sectional area of the collagen fiber bundles of orders II and III, the width of the endotenonium interlayers, the number of ovular capillary network within the functional unit, the number of tendon fibers "waves" per unit length. All this allows us to consider the tissue of the tendon, not inert, but a labile biological system that can adapt to functional load due to changes in morphological indicators.
这项工作分析了马手指肌腱的研究结果,并提出了它们的结构组织的一般模式。结果表明,致密的肌腱结缔组织具有束状结构,其结构中有细胞(成腱细胞和肌腱细胞)和以胶原纤维束为主的细胞间物质,与形成内筋膜和周筋膜的松散结缔组织紧密相连。反过来,这些组织共同构成肌腱结构的功能单元,形成作为器官的肌腱的内部结构。在功能单位水平,肌腱组织被重建。根据我们的数据,允许肌腱实现结构和代谢适应生物力学负荷的最不稳定的连接可能是二级胶原纤维束,它们在形态学(横截面形状)和形态学(横截面面积)指标的可变性上有所不同。肌腱组织通过改变可以量化的结构来适应不同的机械负荷。因此,它改变了II级和III级胶原纤维束的横截面积,内筋间层的宽度,功能单元内卵形毛细血管网络的数量,单位长度的肌腱纤维“波”的数量。所有这些都让我们认为肌腱组织不是惰性的,而是一个不稳定的生物系统,可以适应由于形态指标的变化而产生的功能负荷。
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引用次数: 0
The effectiveness of the use of microalgae in the diets of lactating cows and their effect on milk production and blood biochemical parameters 泌乳奶牛饲粮中添加微藻的效果及其对产奶量和血液生化指标的影响
Pub Date : 2023-01-01 DOI: 10.36871/vet.zoo.bio.202310012
Nina M. Kurilova, Maxim V. Syrovatsky, Alexei A. Vasiliev, Evgeny N. Vaulin
The study of the effect of various feed additives in the diet of lactating cows is an urgent problem, since during this period of production use, animals experience increased loads - recovery after calving, negative energy balance of live weight, decreased appetite, high loads on milk synthesis. The aim of our research was to study the effect of a suspension of chlorella grown in an automatic mobile plant on the milk productivity of first-calf heifers and young cows at the beginning of lactation. We used zootechnical and biochemical studies. First-calf heifers and cows of the second lactation included 1 liter of liquid suspension of chlorella, grown according to the new, patented, technology of Amfor LLC. Monitoring of daily milk yield was carried out weekly during the first phase of lactation. The inclusion of chlorella suspension had a positive effect on milk synthesis. The addition of chlorella suspension effectively affected the daily milk yield of cows from the ninth to the twelfth week of lactation. Starting from the thirteenth week of lactation, the daily milk yield of cows in the experimental and control groups decreased, but remained at a high level. Biochemical studies of the blood of cows in the first phase of lactation did not reveal significant changes in metabolism. No metabolic diseases were detected by the veterinary service.
研究各种饲料添加剂对泌乳奶牛日粮的影响是一个迫切需要解决的问题,因为在这一生产使用时期,动物经历了产犊后恢复负荷增加,活重负能量平衡,食欲下降,奶合成负荷高。本研究的目的是研究在自动移动装置中培养的小球藻悬浮液对初乳母牛和乳牛泌乳初期产奶量的影响。我们使用了动物技术和生物化学研究。第一次犊牛和第二次泌乳的奶牛加入1升小球藻液体悬浮液,根据Amfor LLC的新专利技术生长。泌乳第一阶段每周进行日产奶量监测。小球藻悬浮液的加入对乳汁的合成有积极的影响。添加小球藻悬浮液对奶牛泌乳第9 ~ 12周的日产奶量有显著影响。从泌乳第13周开始,试验组和对照组奶牛的日产奶量均有所下降,但仍保持在较高水平。乳牛泌乳第一阶段血液生化研究未发现代谢有显著变化。兽医部门未发现代谢性疾病。
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引用次数: 0
Comparative evaluation of nutrient media based on protein hydrolysates 基于蛋白质水解物的营养培养基的比较评价
Pub Date : 2023-01-01 DOI: 10.36871/vet.zoo.bio.202309006
Yuriy S. Ovsyannikov, Maxim S. Dursenev
The problem of the choice of protein raw materials and the method of its hydrolysis for the creation of new protein hydrolysates has not yet lost its relevance. The purpose of the work: to study the peculiarities of obtaining enzymatic and acid hydrolysates of embryonic mass, vaccine production waste and to conduct a comparative assessment of the effect of the hydrolysis method on the physico-chemical characteristics, peptide and amino acid composition of the resulting hydrolysates. To obtain hydrolysates, 12-day-old chicken embryos were used as raw materials, after sucking off the virus-containing allantois fluid, are waste products of anti-influenza medication. As hydrolyzing agents, the pancreatic glands of cattle State Standart 11285-93 were used for enzymatic hydrolysis, and synthetic hydrochloric acid State Standart 857-95 was used for acid hydrolysis. It was found that the degree of cleavage in all hydrolysates is average. The content of total and amine nitrogen in the enzyme and initial acid hydrolysates is in the same range, and the values of the same indicators in the acid deionized hydrolysate are significantly less. This is due to the dilution of the hydrolysate during deionization and does not affect its quality. In the studied hydrolysates, the presence of peptides of different molecular weights was noted: in the enzyme hydrolysate, the content of peptides with a molecular weight of 1000 to 2000 Daltons is almost 2.5 times greater than in the acidic one, the level of free amino acids is also higher in the enzyme hydrolysate, and peptides with a molecular weight of 500 to 1000 Daltons in it is 6 times less. The data of the amino acid profile of hydrolysates indicates that the total amount of amino acids in both hydrolysates does not differ significantly. However, all the essential amino acids are present in the enzymatic hydrolysate, and there is no proline and tryptophan in the acid hydrolysate. Thus, the enzymatic and acidic hydrolysis method makes it possible to effectively break down the protein of the embryonic mass and obtain final products with an average degree of protein cleavage. However, the distribution of peptides by fractions and the amino acid composition of hydrolysates varies from the method of its preparation. This circumstance should be taken into account when designing nutrient media for a particular microorganism, taking into account its needs.
选择蛋白质原料及其水解方法以产生新的蛋白质水解物的问题尚未失去其相关性。这项工作的目的是:研究获得胚胎质量、疫苗生产废物的酶解物和酸解物的特点,并对水解方法对所得到的水解物的理化特性、肽和氨基酸组成的影响进行比较评估。为获得水解物,以12日龄的鸡胚为原料,在吮吸含病毒的尿囊液(抗流感药物的废物)后获得水解物。水解剂采用牛胰腺国标1185 -93进行酶解,合成盐酸国标857-95进行酸解。发现所有水解产物的解理程度都是平均的。酶解物和初始酸解物中总氮和胺态氮含量在相同的范围内,酸解物中相同的指标值明显更少。这是由于去离子过程中水解产物被稀释,不影响其质量。在所研究的水解产物中,发现了不同分子量多肽的存在:在酶水解产物中,分子量为1000 - 2000道尔顿的多肽的含量几乎是酸性多肽的2.5倍,游离氨基酸的含量也较高,而分子量为500 - 1000道尔顿的多肽则少了6倍。水解产物的氨基酸谱数据表明,两种水解产物的氨基酸总量差异不显著。然而,所有必需氨基酸都存在于酶解物中,而在酸水解物中没有脯氨酸和色氨酸。因此,酶解和酸性水解方法可以有效地分解胚胎团的蛋白质并获得具有平均蛋白质裂解程度的最终产物。然而,多肽的组分分布和水解产物的氨基酸组成因其制备方法而异。在为特定微生物设计营养培养基时,应考虑到这种情况,考虑到它的需要。
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引用次数: 0
Characteristics of the composition of the parasitofauna of the gastrointestinal tract of ruminants, taking into account the use of anthelmintics 反刍动物胃肠道寄生动物群的组成特点,考虑到驱虫药的使用
Pub Date : 2023-01-01 DOI: 10.36871/vet.zoo.bio.202309014
Olga D. Kirillova, Irina I. Tsepilova, Elizaveta A. Nikolaeva
The article presents data on the composition of the parasitofauna of the gastrointestinal tract in various agricultural ruminants belonging to private farms located on the territory of the Moscow region. Studies have shown that cattle are invaded by 11,45 %, goats by 91,11 % and sheep by 94,12 %. The parasitofauna is represented by the suborder Strongylata, two genera of nematodes – Nematodirus and Trichocephalus, as well as the genus of protozoa Eimeria. The results of the studies indicate the spread of mixed invasions, among which the dominant and permanent articulation are strongylatoses of the gastrointestinal tract. During deworming, benzimidazole- and avermectin- containing drugs were used. The extensivity (EE) of fenbendazole with respect to strongylatoses of the gastrointestinal tract of cattle and goats was 100 %, and EE of ivermectin with respect to strongylatoses of the gastrointestinal tract of goats was 96,56 % and with respect to trichocephalosis 83,33 %, and in sheep 100 % and 66,67 %, respectively.
本文介绍了在位于莫斯科地区的私人农场的各种农业反刍动物胃肠道的寄生动物群组成的数据。研究表明,牛的入侵率为11.45%,山羊的入侵率为91%,绵羊的入侵率为94.12%。寄生动物群的代表是圆线虫亚目、线虫和头毛线虫两属,以及原生动物艾美耳虫属。研究结果表明,混合侵袭的传播,其中主要和永久性的关节是胃肠道的强线虫病。在驱虫过程中,使用含苯并咪唑和阿维菌素的药物。芬苯达唑对牛、山羊胃肠道强线虫病的治疗效果为100%,伊维菌素对山羊胃肠道强线虫病的治疗效果为96.56%,对毛头病的治疗效果为83.3%,对绵羊的治疗效果为100%,对绵羊的治疗效果为66.67%。
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引用次数: 0
Prevalence of acute intestinal infections in calves and pigs in the Krasnodar region 克拉斯诺达尔地区犊牛和猪急性肠道感染的流行情况
Pub Date : 2023-01-01 DOI: 10.36871/vet.zoo.bio.202310008
Andrey G. Koshchaev, Oleg Yu. Chernykh, Alexander S. Tishchenko, Alina Yu. Manakova, Pavel P. Yakovenko, Dmitry O. Alferov
The scientific article presents statistical data on the results of laboratory studies for the presence of pathogenic microorganisms – causative agents of acute intestinal infections among young cattle and pigs in the Krasnodar Territory in the period from 2008 to 2022. The materials used to write the article were provided by GBU «Kropotkin Regional Veterinary Laboratory». In our work, the most frequently reported intestinal diseases of calves and piglets were highlighted, including escherichiosis, enterococcosis, staphylococcosis, streptococcosis, anaerobic enterotoxemia, citrobacteriosis, pseudomoniasis and edematous disease of piglets. A brief description of the pathogens was also given, the most common types of pathogenic bacteria were mentioned. Based on the available data, the frequency of occurrence of acute intestinal diseases was calculated, the percentage of registration of the most common pathogens of infections was established. It has been established that one of the most common acute intestinal pathologies among young cattle and pigs in the Krasnodar Territory over the past 15 years is escherichiosis. Pathogenic E. coli in pathological material was isolated in 41 % of cases in calves, and in 47 % in piglets. Enterococcal infection accounted for 43,3 % of studies in calves and 28,3 % in piglets. Pseudomoniasis, anaerobic enterotoxemia in calves, as well as edematous disease, staphylococcosis and citrobacteriosis in piglets have not been massively registered recently, but are manifested by sporadic outbreaks. Taking into account the current epizootic situation in the Krasnodar Territory for acute intestinal diseases in calves and piglets, it is necessary to conduct regular monitoring of them among the livestock, maintain the veterinary and sanitary condition of livestock farms at the proper level and seek effective, safe and environmentally friendly methods of controlling pathogens.
这篇科学文章介绍了2008年至2022年期间克拉斯诺达尔地区幼牛和猪急性肠道感染病原体病原微生物存在的实验室研究结果的统计数据。撰写本文所用的材料由GBU“克鲁泡特金地区兽医实验室”提供。在我们的工作中,强调了最常见的犊牛和仔猪肠道疾病,包括大肠杆菌病、肠球菌病、葡萄球菌病、链球菌病、厌氧肠毒血症、柠檬酸杆菌病、假单胞菌病和仔猪水肿病。并对病原菌进行了简要介绍,指出了最常见的致病菌类型。根据现有数据,计算急性肠道疾病的发生频率,确定最常见的感染病原体的登记百分比。已经确定,在过去15年中,克拉斯诺达尔地区年轻牛和猪中最常见的急性肠道疾病之一是埃希菌病。在41%的犊牛和47%的仔猪的病理材料中分离出致病性大肠杆菌。小肠球菌感染占犊牛研究的43.3%,占仔猪研究的28.3%。假单胞菌病、犊牛厌氧性肠毒素血症以及仔猪水肿病、葡萄球菌病和柠檬酸杆菌病最近没有大规模登记,但以零星暴发的形式表现出来。考虑到目前克拉斯诺达尔地区犊牛和仔猪急性肠道疾病的动物流行病情况,有必要对牲畜进行定期监测,保持牲畜养殖场的兽医和卫生条件在适当水平,并寻求有效、安全和环保的控制病原体的方法。
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