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Development of a program for calculation of irrigation rates by available moisture reserves 根据可用水分储备计算灌溉速率的程序的开发
Pub Date : 2023-09-25 DOI: 10.28983/asj.y2023i9pp139-143
Boris Viktorovich Fisenko, Olga Valentinovna Mikheeva, Ivan Aleksandrovich Mikheev
The article presents the development of a program for calculating irrigation rates using available moisture reserves.
文章提出了一个程序的发展计算灌溉率利用有效水分储备。
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引用次数: 0
DNA testing of allelic polymorphism of IGF-1, GDF 5, GH, GHR genes in young beef cattle 幼肉牛IGF-1、GDF - 5、GH、GHR基因等位基因多态性的DNA检测
Pub Date : 2023-09-25 DOI: 10.28983/asj.y2023i9pp82-87
Zakir Kamilovich Gajiyev, Evgeniya Semenovna Surzhikovа, Darya Dmitrievna Evlagina, Alexander Ivanovich Surov, Svetlana Nikolaevna Shumaenko
The development and improvement of methodologies that allow assessing the features of the gene pool of domestic, as well as local breeds, farm animals by breeding-significant characteristics for associative research is of both fundamental and applied importance. To carry out breeding work with cattle to increase meat productivity (breeding and commodity farms), it is advisable to consider DNA genotyping as an additional criterion for breeding and selection of animals. In this study, using PCR-PDRF analysis, the frequency of occurrence of the allele spectrum of genes was determined: insulin-like growth factor-1 (IGF?1), differentiating growth factor (GDF5), somatotropin (GH ? growth hormone), growth hormone receptor (GHR), in order to study the breed and population characteristics of repair young cattle cattle of different breeds: Hereford and Kazakh white-headed, bred in farms of the Stavropol Territory. In the course of the study, complex genotypes were identified, the allelic profile of which is represented by eight and seven desirable alleles of four genes in replacement young animals of the Kazakh white-headed breed and six marker alleles of three and four genes in the Hereford breed. It was also noted that the heterozygosity test in the two studied samples of animals for all genes was negative and ranged from -0.10 to -0.44.
发展和改进方法,以便通过对关联研究的育种显著特征来评估国内和地方品种、农场动物基因库的特征,具有基础和应用的重要性。为了开展牛的育种工作以提高肉品产量(育种和商品农场),建议考虑将DNA基因分型作为育种和选择动物的附加标准。本研究通过PCR-PDRF分析,确定了胰岛素样生长因子-1 (IGF -1)、分化生长因子(GDF5)、生长激素(GH ?生长激素),生长激素受体(GHR),以研究不同品种的修复幼牛的品种和种群特征:赫里福德和哈萨克白头牛,饲养在斯塔夫罗波尔地区的农场。在研究过程中,鉴定出复杂的基因型,哈萨克白头品种替代幼畜的等位基因谱由4个基因的8个和7个理想等位基因代表,赫里福德品种的等位基因谱由3个和4个基因的6个标记等位基因代表。还注意到,在两个研究的动物样本中,所有基因的杂合性测试均为阴性,范围为-0.10至-0.44。
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引用次数: 0
Features of calves’ meat productivity formation with different energy nutritional value of forage 不同能量营养价值饲料对犊牛肉产能形成的影响
Pub Date : 2023-09-25 DOI: 10.28983/asj.y2023i9pp76-81
Anatoly Anatolievich Velmatov, Alexander Mikhailovich Guryanov, Anatoly Рavlovich Velmatov
An increase in the level of nutrients in calves diets with a 87.5% Holstein blood genotype by 10-20% compared with the Animal Science Center norms contributes to a significant (P? 0.999) increase in live weight (by 39-73.8 kg). By carcass weight, the bulls of the first and second experimental groups outperformed analogues from the control group by 26.0–52.1 kg (P?0.999), by carcass yield by 2.41–3.92% (P?0.999), by slaughter yield 2.39–4.0% (P?0.999). According to the yield of internal fat, the superiority of the bulls of the first and second experimental groups in comparison with the control group was 0.44–1.64 kg, or 5.2–19.4%. The bulls of the second experimental group differed in their hams and developed musculine torso. The meat index of the experimental bulls is higher compared to the control group by 0.13–0.39%. An increase in the level of feeding by 10-20% in bulls’ diets contributes to a significant increase by 21.1-34.7% in protein and fat carcasses. Hence, the optimization of breeding system and feeding conditions for Holsteinized animals allows maximizing their genetically determined productivity potential.
与动物科学中心标准相比,荷斯坦血型基因型为87.5%的犊牛饲粮中营养物质水平提高了10-20%,显著提高了(P?0.999),增加活重39 ~ 73.8 kg。以胴体重计,1、2试验组公牛比对照组高出26.0 ~ 52.1 kg (P 0.999),比胴体产量高出2.41 ~ 3.92% (P 0.999),比屠宰产量高出2.39 ~ 4.0% (P 0.999)。按内膘量计算,第一、第二试验组公牛较对照组优势0.44 ~ 1.64 kg,优势幅度为5.2 ~ 19.4%。第二个实验组的公牛在他们的火腿和发达的躯干肌肉上有所不同。试验公牛的肉质指数较对照组提高0.13-0.39%。饲粮中饲喂水平每提高10 ~ 20%,可使胴体蛋白质和脂肪显著提高21.1 ~ 34.7%。因此,对饲养系统和饲养条件进行优化,使其基因决定的生产力潜力最大化。
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引用次数: 0
Evaluation of allelic polymorphism of apple tree resistance genes to scab 苹果树抗赤霉病基因等位基因多态性评价
Pub Date : 2023-09-25 DOI: 10.28983/asj.y2023i9pp46-51
Elena Vladimirovna Ulyanovskaya, Evgenia Anatolievna Chernutskaya, Ilnur Malikovich Balapanov, Sergey Vyacheslavovich Tokmakov
The article presents the data of molecular genetic analysis for several genes of the Rvi system of resistance to Venturia inaequalis (Cooke) G. Winter (Rvi1, Rvi6, Rvi8, Rvi9, Rvi11, Rvi14, Rvi16, Rvi17) of the collection and new breeding material of the apple tree (Malus ? domestica Borkh.) of different ploidy, ecological, geographic and genetic origin. We used the CTAB method, which was previously modified in FSBSI NCFSCHVW for more complete purification of DNA samples during extraction. SSR-markers and SCAR-markers linked to the desired 9 genes were used: Rvi1 – CH01D03; Rvi4 – CH02c02a; Rvi6 – CH-Vf1, VfC; Rvi8 – OPL19; Rvi9, Rvi11 – CH05e03; Rvi14 – HB09; Rvi16 – NH030a; Rvi17 – CH-Vf1. The experiment was carried out with the participation of 53 apple samples, including: 17 varieties, 4 elite and 32 hybrid forms of domestic breeding. According to the data obtained, the following genes were classified as quite rare in the studied sample of samples: Rvi9, Rvi16; the presence of genes has not been established: Rvi8, Rvi11, Rvi17. Revealed that among the new breeding material of the apple tree, the Rvi6 gene is the most common, its frequency of occurrence is 62.5%. The study of the allelic polymorphism of several target genes of the Rvi set made it possible to identify promising apple samples for breeding use – carriers of 2–3 resistance genes to Venturia inaequalis (Cooke) G. Winter. According to the DNA marking data, varieties and forms of domestic breeding were selected: Zarya Stavropol'ya, Nika, 12/2-20-75, 17/1-6-72 (carriers of the Rvi1, Rvi6, Rvi14 genes) as promising donors to enhance the efficiency of apple breeding technology for long-term resistance to scab. The data obtained are important for the formation of an identified collection of Malus Mill., the selection of new valuable donors to optimize a number of key stages of the breeding process for long-term resistance to the pathogen by pyramiding the target genes.
本文介绍了苹果(Malus ? ?)采收和新选育材料中拮抗黄萎病(Venturia inaequalis (Cooke) G. Winter (Rvi1、Rvi6、Rvi8、Rvi9、Rvi11、Rvi14、Rvi16、Rvi17) Rvi系统几个基因的分子遗传分析资料。不同倍性、生态、地理和遗传来源的家蝇。我们使用了CTAB方法,该方法先前在FSBSI NCFSCHVW中进行了修改,以便在提取过程中更完整地纯化DNA样品。使用与所需9个基因连接的ssr标记和scar标记:Rvi1 - CH01D03;Rvi4 - CH02c02a;Rvi6 - CH-Vf1, VfC;Rvi8 - OPL19;Rvi9, Rvi11 - CH05e03;Rvi14 - HB09;Rvi16 - NH030a;Rvi17 - CH-Vf1。试验共选材53份,包括17个品种、4个优良品种和32个杂交种。根据获得的数据,在样本的研究样本中,以下基因被归类为相当罕见:Rvi9, Rvi16;Rvi8、Rvi11、Rvi17等基因的存在尚未确定。结果表明,在苹果新选育材料中,Rvi6基因最为常见,其出现频率为62.5%。通过对Rvi组中多个靶基因等位基因多态性的研究,可以鉴定出具有2-3个抗Venturia inaequalis (Cooke) G. Winter基因载体的苹果品种。根据DNA标记数据,选择了具有Rvi1、Rvi6、Rvi14基因载体的扎里亚(Zarya Stavropol’ya)、尼卡(Nika)、12/2-20-75、17/1-6-72作为有希望的供体品种和形式,以提高苹果长期抗痂病育种技术的效率。所获得的数据对于形成一个确定的海棠集合是重要的。,选择新的有价值的供体,以优化育种过程的一些关键阶段,通过对目标基因的金字塔化来长期抵抗病原体。
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引用次数: 0
Estimation of acute oral toxicity of the drug "Mastigard®" 药物“Mastigard®”急性口服毒性评估
Pub Date : 2023-09-25 DOI: 10.28983/asj.y2023i9pp112-115
Kira Vadimovna Solodkova, Azamat Khazretovich Shantyz, Lyudmila Mikhailovna Kashkovskaya
The parameters of acute oral toxicity of Mastigard® are presented. The drug was introduced intragastrically to rats at a dose of 2000 mg/kg. During the study, the tested dose did not cause signs of intoxication and death of animals. Physiological parameters of rats remained within the species norm. Also no changes in the behavior of animals were noted, the reaction to tactile, pain, sound and light irritants was adequate. Autopsy of animals at the end of observation did not reveal pathological and anatomical changes in internal organs and tissues. Regular control of body weight of rats testified to uniform weight gain of all experimental animals during 14 days of observation, no body weight loss was observed during all periods of weighing. Thus, the LD50 of Mastigard® is more than 2000 mg/kg. Mastigard® belongs to the 5th class of toxicity according to the GHS and the 4th class of danger (substances of little danger) according to GOST 12.1.007-76.
给出了Mastigard的急性口服毒性参数。该药物以2000 mg/kg的剂量灌胃给药。在研究过程中,试验剂量没有引起动物中毒和死亡的迹象。大鼠生理参数保持在物种标准范围内。动物的行为也没有变化,对触觉、疼痛、声音和光刺激的反应是足够的。观察结束时对动物进行解剖,未发现内部器官和组织的病理和解剖变化。定期控制大鼠体重,观察14 d期间,所有实验动物体重均均匀增加,称重期间均未见体重下降。因此,Mastigard®的LD50大于2000 mg/kg。根据GHS, Mastigard®属于第5类毒性,根据GOST 12.1.007-76,属于第4类危险(小危险物质)。
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引用次数: 0
Characteristics of the families of the herd of the Sychevka breed by productive and genetic indicators 从生产指标和遗传指标来看,Sychevka品种牧群的家庭特征
Pub Date : 2023-09-25 DOI: 10.28983/asj.y2023i9pp88-97
Mikhail Andreevich Ermakov, Valentina Ivanovna Dmitrieva, Mikhail Eliseevich Gontov, Dmitry Nikolaevich Koltsov
An analysis of the genetic structure using marker alleles of the EAB locus and an assessment of the productive qualities of the families of cows of the Sychevka breed of the pedigree farm KP "Rybkovskoe" of the Smolensk region is presented. In the genotypes of representatives of families with a frequency of more than 5%, there are EAB alleles of the Holstein breed: Y1A`1, G2Y2E`1Q`, O2A`2J`2K`O`, B2O1, and the Sychevka breed: O1I`Q`, b, O1Y2E`3G`G``, which indicates the presence of hereditary material of both breeds. The average milk yield for maximum lactation in cows of the families was 5624kg of milk, with a fat content of 3.91%, protein 3.28%. In the best cows of the families, milk productivity reached 9651kg of milk, 3.71% fat, 3.28% protein for maximum lactation. Thirty percent of descendants from the families of Laskovaya 7114 and Kladi 8080 have a milk yield of over 6000 kg, with a fat content in milk of 3.82% and higher, and protein - over 3.35%. Milk yield in cows, on average in the group by the third lactation compared to the first increases by 970 kg of milk or 22%, which is largely due to the milking of animals in the process of their use. The greatest increase (41%) in milk yield to full-age lactation is observed in animals with the alleles E`3G`G`` (+1681kg) and B1G3O1T1A`1B`E`3F`2Q` (+1706kg). Cows with the EAB allele B1G2O1 are different by an increased (by 40%) period of productive use, and, as a result, lifetime milk yield, they are 42% higher than the average for families. Cows with the marker allele P1QA`1J`2E`1I` have lifetime milk yield indicators 69.5% higher than the average for families.
利用EAB位点的标记等位基因分析了斯摩棱斯克地区“Rybkovskoe”家系农场的Sychevka品种奶牛的遗传结构,并对其生产品质进行了评估。在频率大于5%的家系代表基因型中,荷斯坦品种的EAB等位基因有:Y1A ' 1, G2Y2E ' 1Q ', O2A ' 2J ' 2K ' o ', b201;瑟切夫卡品种的EAB等位基因有:O1I ' q ', b, O1Y2E ' 3G ' g ' ',说明这两个品种都存在遗传物质。各家族奶牛的平均最大泌乳产奶量为5624kg,脂肪含量为3.91%,蛋白质含量为3.28%。最佳奶牛产奶量达到9651kg,脂肪含量为3.71%,蛋白质含量为3.28%,达到最大泌乳量。Laskovaya 7114和Kladi 8080家族中30%的后代产奶量超过6000公斤,牛奶中的脂肪含量为3.82%以上,蛋白质含量超过3.35%。奶牛的产奶量,平均在第三次泌乳组比第一次增加了970公斤奶或22%,这在很大程度上是由于在挤奶过程中对动物的利用。携带E ' 3G ' g ' '和B1G3O1T1A ' 1B ' E ' 3F ' 2Q '等位基因的动物到全龄哺乳期产奶量增加最多(41%)(+1681kg)。携带EAB等位基因B1G2O1的奶牛的不同之处在于,它们的生产利用年限延长了(40%),因此,它们的终生产奶量比家庭平均水平高出42%。带有标记等位基因P1QA ' 1J ' 2E ' 1I '的奶牛一生产奶量指标比家庭平均水平高69.5%。
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引用次数: 0
Openly living phytophages and entomophages of the agrocenosis of winter soft wheat in the forest-steppe of the Samara region 在萨马拉地区的森林草原上,冬软小麦结霉病的公开生活的植噬体和食虫体
Pub Date : 2023-09-25 DOI: 10.28983/asj.y2023i9pp59-65
Ivan Ivanovich Sharapov, Yulia Andreevna Sharapova
Studies were conducted to study the species composition of open-living insects of phytophages and entomophages in the agrocenosis of winter soft wheat on varieties Povolzhskaya 30 and Povolzhskayaniva. The studies were conducted in 2020-2022, which differed in meteorological conditions, in the forest-steppe zone of the Samara region. In the agrocenosis of winter wheat, 28 species of phytophagous insects were identified, which belonged to 16 families. The most numerous in terms of species composition were the families: Chloropidae, Chrysomelidae ,Aphididae, Scutelleridae. The families were small in species composition: Phlaeothripidae, Pentatomidae, Berytidae, Miridae, Acrididae, Elateridae, Cephidae, Carabidae and Scarabaeidae. All phytophagous insects were divided into 6 orders: Bubble-legged or Thrips (Thysanoptera), Hemiptera or bugs (Hemiptera), Diptera (Diptera), Coleoptera or Beetles (Coleoptera), Straight-winged (Ortoptera), Hymenoptera (Hymenoptera). Numerous species composition of phytophagous insects was observed in the orders of Hemiptera (39.0%), Diptera and Coleoptera (25.0% each). The majority of phytophagous insects fed only on vegetative parts of plants (64.0%) (roots, stems, and leaves), 7.0% of species fed on generative parts of plants (mainly grain feeding). 29.0% of the species had a mixed type of nutrition, on vegetative organs (mainly in the larval stage) and on generative organs (mainly imago). Among phytophagous insects, the proportion with a gnawing mouth was 54.0%, and the proportion with a sucking mouth was 46.0%. The species composition of openly living entomophages in the agrocenosis of winter wheat was diverse. It is represented by 6 detachments. The largest number of species was observed in the orders Coleoptera or Beetles (Coleoptera) and the order of Spiders (Araneae). Ladybugs (Coccinellids) dominated among entomophages, which were represented by 9 species.
研究了冬软小麦品种Povolzhskaya 30和Povolzhskayaniva的结瘤病中植食体和食虫体露天昆虫的种类组成。这些研究于2020-2022年在萨马拉地区的森林草原地区进行,气象条件不同。在冬小麦结霉病中,鉴定出食植物昆虫28种,隶属于16科。以种类组成最多的科为:叶蝉科、金蝇科、蚜蝇科、金蝇科。各科种类组成较少,分别为飞虱科、五尾蝇科、蠓科、蠓科、蠓科、蠓科、蠓科、蠓科、蠓科、蠓科。所有食植物昆虫可分为6目:泡足或蓟马(翅翅目)、半翅或小虫(半翅目)、双翅目(双翅目)、鞘翅或小甲虫(翅翅目)、直翅(翅翅目)、膜翅目(膜翅目)。在半翅目(39.0%)、双翅目和鞘翅目(25.0%)中,植食昆虫的种类组成较多。大多数食植物昆虫仅以植物的营养部位(根、茎、叶)为食(占64.0%),7.0%以植物的生殖部位为食(以谷物为食)。29.0%的鱼种以营养器官(主要在幼虫期)和生殖器官(主要在成虫期)为主的混合型营养。植食性昆虫中,咬口占54.0%,吸口占46.0%。冬小麦结霉病中公开活食虫的种类组成多样。它由6个支队代表。以鞘翅目、甲虫目(鞘翅目)和蜘蛛目(蜘蛛目)的种类最多。虫媒昆虫中以瓢虫(Coccinellids)为主,共有9种。
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引用次数: 0
Efficiency of the application of various types of fertilizers in the cultivation of spring durum wheat 春小麦栽培中各种肥料的施用效率
Pub Date : 2023-09-25 DOI: 10.28983/asj.y2023i9pp66-70
Konstantin Evgenievich Denisov, Ilia Sergeevich Poletaev, Anastasia Aleksandrovna Geraskina, Aleksandr Nikolaevich Nikitin, Elena Borisovna Solovyeva
The work studied the influence of various methods of basic soil cultivation: plowing, minimal tillage and plant nutrition systems: mineral fertilizer ammophos, microbiological fertilizer Azofit and complex mineral fertilizers Strada N, Microel on the productivity of spring durum wheat (Luch 25 variety). In recent years, there has been a steady decline in the area under spring wheat crops, especially durum wheat, in the region, which in turn indicates the need to improve its cultivation, taking into account climatic changes. In the course of the research, it was found out that in dry weather conditions after deep soil cultivation, the most cost effective was the application of a complex nitrogen-containing mineral fertilizer Strada N; during minimal basic tillage, the highest level of profitability was on the variant with the foliar application of complex mineral fertilizer Strada N together with mineral fertilizers.
研究了不同的基础土壤耕作方法:翻耕、少耕和植物营养系统:矿肥氨磷、微生物肥偶氮肥和复合矿肥Strada N、Microel对春小麦(Luch 25)产量的影响。近年来,该地区春小麦,特别是硬粒小麦的种植面积不断减少,这反过来表明需要在考虑到气候变化的情况下改善其种植。在研究过程中发现,在土壤深耕后的干燥天气条件下,施用复合含氮矿物肥Strada N最具成本效益;在最低基本耕作条件下,叶面施用复合矿肥Strada N和矿肥的品种盈利水平最高。
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引用次数: 0
Power balance calculation of a mobile energy vehicle with a mounted adapter for harvesting bast crops 一种装有用于采收韧皮作物的适配器的移动能源车的功率平衡计算
Pub Date : 2023-09-25 DOI: 10.28983/asj.y2023i9pp144-151
Mikhail Evgenievich Chaplygin, Roman Andreevich Popov, Sergey Evgenievich Senkevich
Bast crops are characterized by the content of strong bast fibers in the stems and a significant height of crops. The process of cleaning them involves the use of narrowly focused and powerful machines. The article considers the principle of distribution of the effective power of a self-propelled harvesting unit for bast crops. The evaluation of the components of the power balance will allow quantifying the power capabilities of the engine and the loading of the energy means in the unit with an adapter to perform the technological process for various cleaning conditions
韧皮作物的特点是茎中韧皮纤维含量高,作物高度显著。清理它们的过程涉及到使用聚焦狭窄、功能强大的机器。本文研究了一种自行式农作物收获装置的有效功率分配原理。对功率平衡组件的评估将允许量化发动机的功率能力和能量手段的负载,在具有适配器的单元中执行各种清洁条件下的工艺过程
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引用次数: 0
Effect of different levels of selenium nanoparticles and cobalt asparaginate on safety and productive qualities of calves 不同水平纳米硒和天冬酰胺钴对犊牛安全和生产品质的影响
Pub Date : 2023-09-25 DOI: 10.28983/asj.y2023i9pp98-101
Ivan Isaevich Kalyuzhny, Yaroslav Borisovich Drevko, Sergey Petrovich Moskalenko, Sergey Olegovich Loshchinin, Aleksandr Mephodievich Semivolos
A mineral feed additive in the composition of selenium and cobalt aspartate nanoparticles (OMEC-Co) was tested and an assessment was made of the growth rate and feed costs for calves of the dairy period of growing. As a result of the research, it was found out that calves from the control group grew by 631 g daily. This figure in the calves of the experimental groups was 12 and 18 g more. The difference ensured higher gross growth. Due to this, the calves of the experimental groups used energy and nutrients better and spent 0.06 and 0.09 EFU less compared to their peers from the control group.
试验了一种由天冬氨酸硒钴纳米颗粒(OMEC-Co)组成的矿物饲料添加剂,并对奶牛生长期犊牛的生长速度和饲料成本进行了评估。研究结果显示,对照组的犊牛每天增重631克。实验组小牛的这一数字分别高出12克和18克。这一差额确保了更高的总增长率。因此,实验组犊牛的能量和营养利用率较对照组犊牛高,消耗的EFU分别为0.06和0.09。
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引用次数: 0
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Аграрный научный журнал
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