Arab Journal of Gastroenterology最新文献_第3页

Licoricidin suppresses growth and metastasis of hepatocellular carcinoma by targeting PI3K/AKT signaling. Licoricidin通过靶向PI3K/AKT信号通路抑制肝癌的生长和转移。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-05 DOI: 10.1016/j.ajg.2025.09.018

Xiaosong Li, Zhigang Pan

Background: Licoricidin is a prenylated isoflavone isolated from Glycyrrhiza uralensis Fisch, and its biological activities and pharmacological properties have been reported in recent years. This study comprehensively explored the licoricidin's effect on hepatocellular carcinoma (HCC) and elucidated its underlying mechanism.

Methods: HCC Hep3B and Huh-7 cells were treated with licoricidin at various concentrations. The experimental methods in vitro, including CCK-8, colony formation, EdU, flow cytometry, wound healing, and transwell were performed to evaluate the licoricidin's effect on HCC cell proliferation, apoptosis, migration, and invasion. The antitumor and anti-metastasis activities of licoricidin were validated using a Hep3B xenograft model and a metastasis model. Western blot or RT-qPCR was used to measure the levels of apoptosis-, epithelial-mesenchymal transition-, and PI3K/AKT-related proteins.

Results: The results indicated that the antiproliferation effect of licoricidin in HCC was related to cell cycle arrest (S phase) and apoptosis induction. Licoricidin upregulated Bax and cleaved caspase3/9 expression and downregulated Bcl-2 expression in cancer cells. In a subcutaneous xenograft model, licoricidin treatment suppressed the growth of tumor. Nontoxic concentrations of licoricidin reduced the migration and invasion abilities of HCC cells. Licoricidin downregulated N-cadherin and vimentin expression, while upregulating E-cadherin expression. Licoricidin treatment reduced the number of metastatic lung nodules in a metastasis model. Inhibition of PI3K/AKT signaling might be a potential mechanism behind the licoricidin's anticancer effect.

Conclusion: Overall, licoricidin suppresses growth and metastasis of HCC by targeting. PI3K/AKT signaling, suggesting that licoricidin might be a promising candidate for HCC treatment.

背景：Licoricidin是一种从甘草中分离得到的异丙基化异黄酮，其生物活性和药理性质近年来被报道。本研究全面探讨了licoricidin对肝细胞癌（HCC）的作用，并阐明了其作用机制。方法：用不同浓度的甘草霉素处理肝癌Hep3B和Huh-7细胞。采用CCK-8、菌落形成、EdU、流式细胞术、创面愈合、transwell等体外实验方法，评价licoricidin对HCC细胞增殖、凋亡、迁移和侵袭的影响。通过Hep3B异种移植模型和转移模型验证了甘草霉素的抗肿瘤和抗转移活性。采用Western blot或RT-qPCR检测细胞凋亡-、上皮-间质转化-和PI3K/ akt相关蛋白的水平。结果：甘草霉素在HCC中的抗增殖作用与细胞周期阻滞（S期）和诱导细胞凋亡有关。Licoricidin上调癌细胞中Bax和caspase3/9的表达，下调Bcl-2的表达。在皮下异种移植模型中，甘草霉素治疗抑制了肿瘤的生长。无毒浓度的licoricidin降低了HCC细胞的迁移和侵袭能力。Licoricidin下调N-cadherin和vimentin的表达，上调E-cadherin的表达。在转移模型中，甘草霉素治疗减少了转移性肺结节的数量。抑制PI3K/AKT信号可能是licoricidin抗癌作用的潜在机制。结论：总的来说，甘草霉素通过靶向作用抑制肝癌的生长和转移。PI3K/AKT信号，提示licoricidin可能是HCC治疗的有希望的候选药物。

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引用次数: 0

Effects of oral-gastric treatment and family therapy on H. Pylori eradication and recurrence 口服胃疗及家庭治疗对幽门螺杆菌根除及复发的影响。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 DOI: 10.1016/j.ajg.2025.08.005

Zhiqiang Chen , Fang Yuan , Chao Pan

Background and study aims

Helicobacter pylori (H. pylori) infections are notably difficult to eradicate due to high antibiotic resistance and frequent reinfection. This study evaluates the effectiveness of combining oral and gastric treatments with synchronized family therapy compared to standard therapy for H. pylori management.

Patients and methods

Conducted at Cangshan Hospital of the 900th Hospital of the Joint Logistics Support Force of the Chinese People’s Liberation Army, the study included 180 patients divided into three groups: standard triple therapy (Group A), triple therapy plus oral treatment (Group B), and triple therapy with oral treatment and synchronized family therapy (Group C). Eradication and recurrence were measured at 3 and 6 months using stool antigen tests and 14C-urea breath tests.

Results

Initial eradication rates were similar across the three groups. By 3 months, eradication rates reached 76.7 % in Group A, 86.7 % in Group B, and 93.3 % in Group C. At 6 months, these rates were 61.7 %, 66.7 %, and 90 %, respectively. Concurrently, recurrence rates at 6 months climbed to 33.9 % and 29.8 % in Groups A and B, respectively, but remained low at 5.3 % in Group C.

Conclusion

The addition of synchronized family therapy significantly enhances long-term eradication and reduces recurrence rates, suggesting its potential for incorporation into standard H. pylori treatment protocols.

背景与研究目的：幽门螺杆菌（Helicobacter pylori， H. pylori）感染由于其高耐药性和频繁的再感染而难以根治。本研究评估了与标准治疗相比，口服和胃联合治疗与同步家庭治疗对幽门螺杆菌治疗的有效性。患者和方法：研究在中国人民解放军联勤保障部队第900医院苍山医院进行，纳入180例患者，分为三组：标准三联治疗（A组）、三联治疗加口服治疗（B组）、三联治疗加口服治疗和家庭同步治疗（C组）。3个月和6个月时用粪便抗原试验和14c -尿素呼气试验测定根除和复发率。结果：三组的初始根除率相似。3个月时，A组根除率为76.7%，B组为86.7%，c组为93.3%。6个月时，A组根除率为61.7%，B组为66.7%，c组为90%。同时，A组和B组的6个月复发率分别攀升至33.9%和29.8%，但c组的复发率仍然很低，为5.3%。结论：同步家庭治疗的加入显著增强了长期根除，降低了复发率，表明其有可能纳入标准的幽门螺杆菌治疗方案。

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引用次数: 0

Comments on “Clinical features and temporal trends in H. pylori negative gastric maltoma” 对“幽门螺杆菌阴性胃恶性瘤的临床特征和时间趋势”的评论。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 DOI: 10.1016/j.ajg.2025.07.005

Ali Beheshti Namdar, Masoud Keikha

引用次数: 0

The role of salivary calprotectin in monitoring Crohn’s disease: A cross-sectional study 唾液钙保护蛋白在克罗恩病监测中的作用：一项横断面研究。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 DOI: 10.1016/j.ajg.2025.08.008

Awni Abu Sneineh , Sara Haj Ali , Maryam Ali Alani , Joud Al Karmi , Feras Al Karmi , Mina F. Al-Wandawi , Suzan Al Bdour , Lana Agraib , Rasha Mohammed Ali , Mamoun Ahram

Background and study aims

Noninvasive biomarkers are needed to monitor disease activity in inflammatory bowel disease, as current methods—including blood tests, endoscopy, imaging, and fecal calprotectin—are invasive, costly, or burdensome. This study explored salivary calprotectin as a potential biomarker for Crohn’s disease activity.

Patients and methods

This was a cross-sectional exploratory study. Unstimulated saliva was collected from patients with an established diagnosis of Crohn’s disease and matched healthy controls. Disease activity was determined via the Crohn’s disease Activity Index. Salivary calprotectin was measured in 20 μl saliva samples using enzyme-linked immunosorbent assay.

Results

Samples were collected from 42 patients with Crohn’s disease (mean age 36.3 ± 14.2 years, 52.4 % male) and 41 age-matched healthy controls (mean age 38 ± 13.4 years, 58.5 % male). For patients with Crohn’s disease, the median salivary calprotectin level was 0.08 (IQR 0.05–0.14) ug/ml compared to 0.06 (IQR 0.03–0.13) ug/ml in healthy controls (p = 0.161). Among patients with Crohn’s disease, levels were similar in remission (0.10, IQR 0.05–0.15 μg/mL) and active disease (0.07, IQR 0.03–0.10 μg/mL; p = 0.091). No correlation was observed between salivary calprotectin and Crohn’s disease activity index scores (Spearman’s r = −0.136, p = 0.392).

Conclusions

Salivary calprotectin did not distinguish patients with Crohn’s disease from healthy controls or correlate with disease activity. While noninvasive, it does not appear to be a clinically useful biomarker for monitoring Crohn’s disease. Further research may clarify its role in inflammatory bowel disease or identify more promising biomarkers.

背景和研究目的：需要无创生物标志物来监测炎症性肠病的疾病活动，因为目前的方法——包括血液检查、内窥镜检查、成像和粪便钙保护蛋白——是有创的、昂贵的或负担的。本研究探索唾液钙保护蛋白作为克罗恩病活动性的潜在生物标志物。患者和方法：这是一项横断面探索性研究。从确诊为克罗恩病的患者和匹配的健康对照中收集未受刺激的唾液。通过克罗恩病活动性指数确定疾病活动性。采用酶联免疫吸附法测定20 μl唾液中钙保护蛋白的含量。结果：样本来自42例克罗恩病患者（平均年龄36.3±14.2岁，男性52.4%）和41例年龄匹配的健康对照（平均年龄38±13.4岁，男性58.5%）。克罗恩病患者唾液钙保护蛋白水平中位数为0.08 (IQR 0.05-0.14) ug/ml，而健康对照组为0.06 (IQR 0.03-0.13) ug/ml （p = 0.161）。在克罗恩病患者中，缓解期（0.10,IQR 0.05-0.15 μg/mL）和活动性（0.07,IQR 0.03-0.10 μg/mL, p = 0.091）的水平相似。唾液钙保护蛋白与克罗恩病活动性指数评分无相关性（Spearman’s r = -0.136, p = 0.392）。结论：唾液钙保护蛋白不能区分克罗恩病患者和健康对照者，也不能与疾病活动性相关。虽然无创，但它似乎不是一种临床上有用的监测克罗恩病的生物标志物。进一步的研究可能会阐明其在炎症性肠病中的作用或确定更有希望的生物标志物。

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引用次数: 0

Prevalence of Blastocystis hominis, Cryptosporidium, and Strongyloidesstercoralis among patients with colorectal carcinoma in Egypt 埃及结直肠癌患者中人芽囊虫、隐孢子虫和类圆线虫的患病率。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 DOI: 10.1016/j.ajg.2025.09.003

Yasmine Gaber , Noha Madbouly Taha , Nouran Roby , Hany William , Mohamed Mostafa , Raghda Marzaban

Background and Aim

Colorectal cancer (CRC) is a leading cause of cancer-related death, with strong environmental associations and genetic risk factors. Although some microorganisms have been linked to carcinogenesis in CRC, the prevalence of parasites and their potential role in carcinogenesis remain unclear. This study aimed to determine the prevalence and association of the parasites Blastocystis, Cryptosporidium, and Strongyloides in CRC.

Patients and Methods

A case-control study was conducted, including 120 participants, divided into 60 patients with CRC and 60 healthy controls. Patients were recruited from two tertiary care hospitals in Cairo, Egypt. Parasitologic microscopy-based tests for Blastocystis hominis (B. hominis), Cryptosporidium oocysts, and Strongyloides stercoralis (S. stercoralis) rhabditiform larvae were performed using iodine-stained smears after formol ether concentration, modified Ziehl-Neelsen (MZN) staining, and agar plate culture, respectively.

Results

Blastocystis and Cryptosporidium were detected at comparable rates in the CRC and control groups (26.7 % vs 28.3 %, p = 0.83 and 8.3 % vs 10 %, p = 0.75, respectively), with no significant association with CRC in terms of site, pattern, histological types, or stages (p = 0.92, 0.88, 0.84, and 0.39, respectively). However, most cases of Blastocystis or Cryptosporidium were observed in localized CRC in the rectum of the adenocarcinoma type. S. stercoralis was not detected in the stool examinations of either patients or controls.

Conclusion

None of the parasites studied demonstrated a significant association with CRC when using conventional microscopy-based methods of parasite detection.

背景和目的：结直肠癌（CRC）是癌症相关死亡的主要原因，与环境和遗传因素有很强的相关性。虽然一些微生物与结直肠癌的癌变有关，但寄生虫的流行及其在癌变中的潜在作用尚不清楚。本研究旨在确定囊虫、隐孢子虫和圆线虫等寄生虫在结直肠癌中的患病率及其相关性。患者和方法：进行了一项病例对照研究，包括120名参与者，分为60名结直肠癌患者和60名健康对照。患者从埃及开罗的两家三级保健医院招募。分别用碘染色涂片、改良Ziehl-Neelsen （MZN）染色和琼脂平板培养对人芽囊虫（B. hominis）、隐孢子虫卵囊和粪圆线虫（S. stercoralis）横纹肌状幼虫进行寄生虫学显微镜检测。结果：囊虫和隐孢子虫在结直肠癌组和对照组的检出率相当（分别为26.7%对28.3%,p = 0.83和8.3%对10%，p = 0.75），在部位、模式、组织学类型或分期方面与结直肠癌无显著相关性（p = 0.92、0.88、0.84和0.39）。然而，大多数囊虫或隐孢子虫见于直肠局部结直肠癌的腺癌型。患者和对照组的粪便检查均未检出粪球菌。结论：当使用传统的基于显微镜的寄生虫检测方法时，所研究的寄生虫均未显示出与结直肠癌的显著关联。

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引用次数: 0

Significant association of sensitivity to thyroid hormones with liver fibrosis: Analysis based on NHANES data 甲状腺激素敏感性与肝纤维化的显著关联：基于NHANES数据的分析。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 DOI: 10.1016/j.ajg.2025.09.001

Yan Zhao , Xin-Quan Wu , Pan Luo , Shu-Li Liao

Background and study aims

To explore the association between thyroid function and liver fibrosis using the data from the NHANES database.

Patients and methods

The data of 5959 participants and 34 variables were obtained. Fibrosis-4 (FIB-4) score was used to assess the liver fibrosis level. LASSO regression analysis was conducted to identify the important thyroid function related indicators and other variables. Logistic and linear regression analyses were used to explore the association between key indicators and FIB-4 score, and their association was then verified by RCS and GAM analyses. The stability of their association was evaluated by trend regression and interaction effect analyses.

Results

There were 4652 and 1307 participants in low and high FIB-4 score groups, respectively. LASSO analysis initially identified 17 important variables, of which 4 thyroid indicators were contained including total T3, FT4, TSH, and TT4RI (an index for reflecting sensitivity to thyroid hormones). Of them, TSH and TT4RI showed significant association with liver fibrosis level when FIB-4 was set as categorical (fully adjusted OR_TSH = 1.048 [95 %CI: 1.026, 1.077], P < 0.001; OR _TT4RI = 1.007 [95 %CI: 1.004, 1.011], P < 0.001) or continuous variable (fully adjusted β_TSH = 0.010 [95 %CI: 0.004, 0.015], P = 0.001; β_TT4RI = 0.002 [95 %CI: 0.001, 0.003], P < 0.001). RCS and GAM analyses further supported their association (all fully adjusted P < 0.05). However, fully adjusted trend regression analysis indicated the more stable association of TT4RI with liver fibrosis in a positive relationship (OR_TT4RI = 1.079 [95 %CI: 1.013, 1.150], P = 0.018; β_TT4RI = 0.019 [95 %CI: 0.004, 0.034], P = 0.011). We further found that age, sleep disorder, sodium level, and lymphocyte number had interaction effect with TT4RI (all P for interaction effect < 0.05).

Conclusions

The sensitivity to thyroid hormones was significantly related to the liver fibrosis, which was more valuable than the common thyroid profile.

背景和研究目的：利用NHANES数据库的数据，探讨甲状腺功能与肝纤维化之间的关系。患者与方法：共获得5959名受试者和34个变量的数据。纤维化-4 （FIB-4）评分用于评价肝纤维化水平。采用LASSO回归分析确定甲状腺功能相关重要指标及其他变量。采用Logistic和线性回归分析探讨关键指标与FIB-4评分的相关性，并采用RCS和GAM分析验证其相关性。通过趋势回归分析和交互效应分析，评价二者关联的稳定性。结果：FIB-4低评分组4652例，FIB-4高评分组1307例。LASSO分析初步确定了17个重要变量，其中包含4个甲状腺指标，包括总T3、FT4、TSH和TT4RI（反映甲状腺激素敏感性的指标）。其中，当FIB-4为分类时，TSH和TT4RI与肝纤维化水平有显著相关性（完全校正ORTSH = 1.048 [95% CI: 1.026, 1.077], P TT4RI = 1.007 [95% CI: 1.004, 1.011], P TSH = 0.010 [95% CI: 0.004, 0.015], P = 0.001; βTT4RI = 0.002 [95% CI: 0.001, 0.003], P TT4RI = 1.079 [95% CI: 1.013, 1.150], P = 0.018; βTT4RI = 0.019 [95% CI: 0.004, 0.034], P = 0.011）。我们进一步发现年龄、睡眠障碍、钠水平和淋巴细胞数量与TT4RI存在交互作用（交互作用均为P）。结论：甲状腺激素敏感性与肝纤维化有显著相关性，比普通甲状腺谱更有价值。

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引用次数: 0

Predictors of antiviral treatment in Chronic Hepatitis −B (CHB) population 慢性乙型肝炎（CHB）人群抗病毒治疗的预测因素。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 DOI: 10.1016/j.ajg.2025.09.008

Tanu Yadav, Suchit Swaroop

Background

CHB causes liver adversity and can be treated by antiviral drugs. HBV-DNA with Liver enzymes, HBeAg and, HBeAb plays a very crucial role in determining whether to provide treatment or not.

Objective

This study aimed to derive the prognostic impact of HBeAb on CHB infection and the association between HB viral antigen, HB antibodies, and liver enzymes in providing antiviral treatment to CHB patients.

Methods

This study was performed on all HBsAg-positive patients. A data extraction form was prepared to analyse biochemical measures associated with antiviral treatment.

Results

An association between antiviral drugs and viral antigens was reported. The average age of participants was 36.89 (± 13.703), and separately, males were 37.64583 (±14.501), and females were 35.2093 (±11.708). Mean of ALT level 105.788 (±239.9647) and AST level 94.775 (± 234.0753). The highest HBeAg positivity was reported at the age of 15–30 years. 0.822 and 0.723p values were reported for the association between ALT, AST serum levels and HBeAg. HBeAg and antiviral therapy show a 0.022 significant value. Regression analysis reported HBeAg with different variables: age (p = 0.422), gender (p = 0.780), ALT (p = 0.819), and AST (p = 0.439).

Conclusion

Various biomarkers play an important role in treating HBsAg-positive patients. This study stated the pointers to be incorporated during the treatment of HBV patients.

Abbreviation: HBV, Hepatitis- B Virus; CHB, Chronic Hepatitis-B; HBsAg, Hepatitis-B surface Antigen; HBeAg, Hepatitis-B envelope antigen; HBeAb, Hepatitis-B envelope antibody.

背景：慢性乙型肝炎引起肝脏病变，可通过抗病毒药物治疗。HBV-DNA与肝酶、HBeAg和HBeAb在决定是否提供治疗方面起着非常关键的作用。目的：本研究旨在探讨乙型肝炎病毒抗体（HBeAb）对慢性乙型肝炎（CHB）感染的预后影响，以及乙型肝炎病毒抗原、乙型肝炎抗体和肝酶之间的关系，从而为慢性乙型肝炎患者提供抗病毒治疗。方法：本研究在所有hbsag阳性患者中进行。制备数据提取表，分析与抗病毒治疗相关的生化指标。结果：报道了抗病毒药物与病毒抗原的相关性。参与者平均年龄36.89（±13.703）岁，其中男性37.64583（±14.501）岁，女性35.2093（±11.708）岁。ALT水平均值105.788（±239.9647），AST水平均值94.775（±234.0753）。HBeAg阳性率最高的年龄为15-30岁 岁。血清ALT、AST水平与HBeAg的相关性分别为0.822和0.723p。HBeAg与抗病毒治疗有0.022的显著性差异。回归分析结果显示，HBeAg与年龄（p = 0.422）、性别（p = 0.780）、ALT（p = 0.819）、AST （p = 0.439）相关。结论：多种生物标志物在治疗hbsag阳性患者中发挥重要作用。本研究阐述了HBV患者治疗过程中应注意的事项。简称：HBV，乙型肝炎病毒；慢性乙型肝炎；乙型肝炎表面抗原；乙型肝炎包膜抗原；乙型肝炎包膜抗体。

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引用次数: 0

Evaluation of linked-color imaging endoscopy combined with CAD EYE for the detection and characterization of right-sided colon polyps 彩色内镜联合CAD EYE对右侧结肠息肉的检测及特征评价。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 DOI: 10.1016/j.ajg.2025.09.016

Phuoc Huu Tran , Thi Huyen Thuong Nguyen , Van Huy Tran

Background and study aims: Optimizing polyp detection, in particular the right-sided colon polyps, is a challenge for endoscopists. This study aimed to evaluate the diagnostic value of LCI combined with CAD EYE in the detection and characterization of right-sided colon polyps.

Patients and methods

Patients underwent colonoscopy with WLI up to the cecum and terminal ileum, followed by LCI combined with CAD EYE. All polyps detected were removed and subjected to histological examination.

Results

116 patients were enrolled. Out of them, 66 patients had at least one polyp in their right-sided colon. Endoscopy utilizing LCI resulted in a significantly high additional polyp detection rate (APDR) of 25.9 % (95 % CI: 17.6–36.4 %) in the right colon compared to endoscopy with WLI. The APDRs were significantly higher for flat polyps, 43.5 % (95 % CI: 25.6–63.2 %), p = 0.023. The adenoma detection rate (ADR) was also increased, with APDRs for adenomatous polyps and sessile serrated polyps at 30.6 % (95 % CI: 18.0–46.9 %) and 66.7 % (95 % CI: 30–90.3 %), respectively (p = 0.022). The concordance in neoplasia detection utilizing LCI combined with CAD EYE and histological examination demonstrated substantial agreement between the two approaches, with Kappa values of 0.729 (p < 0.001).

Conclusion

LCI combined with CAD EYE emerged as a potential tool for characterizing right-sided colon polyps.

背景和研究目的：优化息肉的检测，特别是右侧结肠息肉，是内镜医师面临的挑战。本研究旨在评价LCI联合CAD EYE对右侧结肠息肉的诊断价值。患者和方法：患者行WLI至盲肠和回肠末结肠镜检查，然后行LCI联合CAD EYE。所有检测到的息肉均被切除并进行组织学检查。结果：116例患者入组。其中，66名患者的右侧结肠至少有一个息肉。与使用WLI的内镜相比，使用LCI的内镜在右结肠的额外息肉检出率（APDR）显著高，为25.9% （95% CI: 17.6- 36.4%）。扁平息肉的apdr为43.5% (95% CI: 25.6 ~ 63.2%), p = 0.023。腺瘤检出率（ADR）也有所提高，腺瘤性息肉和无根锯齿状息肉的apdr分别为30.6% （95% CI: 18.0 ~ 46.9%）和66.7% (95% CI: 30 ~ 90.3%) （p = 0.022）。LCI联合CAD EYE的肿瘤检测结果与组织学检查结果一致，Kappa值为0.729 (p)。结论：LCI联合CAD EYE是诊断右侧结肠息肉的潜在工具。

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引用次数: 0

Frailty and MASLD in the Arab region: An urgent call for regional clinical and public health strategies 阿拉伯区域的虚弱和MASLD：紧急呼吁制定区域临床和公共卫生战略。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 DOI: 10.1016/j.ajg.2025.07.003

Mohamed El-Kassas , Khalid Alswat , Asma Labidi , Maen Almattooq , Doaa Zakaria , Nabil Debzi , Faisal M Sanai , Mohamed Elbadry , Ali Tumi , Imam Waked , Abdel-Naser Elzouki , Khalid M. AlNaamani

引用次数: 0

Investigation of the biomarkers and mechanism based on endoplasmic reticulum stress in irritable bowel syndrome 肠易激综合征内质网应激的生物标志物及机制研究。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 DOI: 10.1016/j.ajg.2025.08.004

Zhenyu Fan , Jiufang Yao , Xiayang Zeng , Jinhong Zuo , Jianpeng Qu

Background and study aims

Irritable bowel syndrome (IBS) is a common bowel dysfunction disorder. Endoplasmic reticulum (ER) stress plays a key role in the pathogenesis of several gastrointestinal diseases. This study investigated important ER stress-related genes (ERGs) and their mechanisms of action in IBS.

Material and methods

IBS and ERGs data were retrieved from public databases. Differential expression analysis, Venn analysis, and three machine learning algorithms, the most important ERGs in IBS were selected. A nomogram model was then constructed for the clinical predictions based on optimal ERGs. Finally, an LPS-induced Caco-2 cell line was established to simulate intestinal barrier damage and validate the expression of key genes and their underlying mechanisms.

Results

Six key ERGs (EGFR, MET, INSR, RETREG1, MCL1, and BSG) were selected to construct the nomogram model, which showed promising clinical predictive performance. These six genes were significantly correlated with immune response and ER stress-related pathways, such as oxidative phosphorylation. In vitro cell experiments have demonstrated damaged barrier function and ER stress activation in IBS, manifesting as lower transepithelial resistance, higher cell permeability, higher levels of inflammatory cytokines, and higher levels of ER proteins in LPS-induced cells. Furthermore, MET expression was significantly increased in the LPS group, whereas EGFR, INSR, RETREG1, MCL1, and BSG levels were significantly decreased in the LPS group.

Conclusion

Our study demonstrated a six-gene nomogram model for IBS based on ER stress, which showed a strong relationship between ER stress and various diseases.

背景与研究目的：肠易激综合征（Irritable bowel syndrome， IBS）是一种常见的肠功能障碍。内质网应激在多种胃肠道疾病的发病机制中起关键作用。本研究探讨了肠内质网应激相关基因及其在肠易激综合征中的作用机制。材料和方法：IBS和ERGs数据从公共数据库检索。选择了IBS中最重要的ERGs，采用差分表达分析、维恩分析和三种机器学习算法。然后建立基于最佳ergg的nomogram模型进行临床预测。最后，建立lps诱导的Caco-2细胞系，模拟肠屏障损伤，验证关键基因的表达及其机制。结果：选取EGFR、MET、INSR、RETREG1、MCL1、BSG 6个关键ERGs构建nomogram模型，具有良好的临床预测效果。这6个基因与免疫应答和内质网应激相关通路（如氧化磷酸化）显著相关。体外细胞实验证明IBS中屏障功能受损和内质网应激激活，表现为经皮阻力降低，细胞通透性提高，炎症细胞因子水平升高，lps诱导的细胞内质网蛋白水平升高。此外，LPS组MET表达显著升高，而EGFR、INSR、RETREG1、MCL1和BSG水平显著降低。结论：我们的研究建立了基于内质网应激的IBS六基因形态图模型，该模型显示了内质网应激与多种疾病之间的密切关系。

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引用次数: 0