Arab Journal of Gastroenterology最新文献_第7页

Quality of life and depressive state in patients with celiac disease: A case-control study 乳糜泻患者的生活质量和抑郁状态：一项病例对照研究

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-05-08 DOI: 10.1016/j.ajg.2025.03.004

Irem Unalcin , Suleyman Ersoy , Emin Pala , Velittin Selcuk Engin

Background and study aims

The objective of this study was to evaluate the quality of life (QoL) of celiac patients, their predisposition to depression, and possible related factors.

Patients and methods

The study was planned as a case-control study and conducted between May 2023 and August 2023 at Umraniye Training and Research Hospital, Istanbul. The study included 81 patients with celiac disease (CD) aged 18–65 years who were followed up at the Gastroenterology outpatient clinic and 79 healthy controls who were admitted to the Family Medicine Outpatient Clinic. Participants were administered the Celiac Disease Questionnaire (CDQ), the World Health Organization Quality of Life Scale-Short Form (WHOQOL-BREF), and the Beck Depression Inventory (BDI). Statistical analysis was performed using SPSS 24 (Statistical Package for the Social Sciences).

Results

The study was conducted with 160 participants, of whom 73.8 % (n = 118) were female and 26.3 % (n = 42) were male. Age and gender distributions were similar. The celiac group had significantly lower scores of the WHOQOL-BREF “Overall Health” subscale compared to healthy controls (p = 0.004, Cohen’s d = 0.46). However, no significant differences were observed in other WHOQOL-BREF subscales or depression levels (p > 0.05). In the celiac group, higher education levels (undergraduate and postgraduate) were associated with better QoL scores (η² = 0.11, p = 0.015), while lower education was linked to higher depression scores (p = 0.019). A strong negative correlation was found between CDQ scores and BDI scores (r = -0.529, p = 0.001), indicating that higher depression levels were associated with lower QoL.When controlled for confounding variables, gender and CD turned out to be independently related to overall health subscale scores.

Conclusion

In patients with CD, both the QoL scale CDQ and the WHOQOL-BREF scores were found to be ahead of the curve in our study. It was also observed that the WHOQOL-BREF scale “overall health” subscale score was lower in celiac patients, while other sub-parameters and depressive symptoms were comparable to healthy individuals The lower “Overall Health” subscale scores highlight the need for targeted interventions.

背景与研究目的：本研究的目的是评估乳糜泻患者的生活质量、抑郁易感性及其可能的相关因素。患者和方法：该研究计划作为病例对照研究，于2023年5月至2023年8月在伊斯坦布尔Umraniye培训和研究医院进行。该研究包括81名年龄在18-65岁之间的乳糜泻（CD）患者，他们在胃肠病学门诊接受随访，79名健康对照者在家庭医学门诊接受随访。参与者接受了乳糜泻问卷（CDQ）、世界卫生组织生活质量量表-短表（WHOQOL-BREF）和贝克抑郁量表（BDI）。使用SPSS 24 （Statistical Package for Social Sciences）进行统计分析。结果：共纳入160例受试者，其中女性118例（73.8%），男性42例（26.3%）。年龄和性别分布相似。与健康对照组相比，乳糜泻组的WHOQOL-BREF“整体健康”亚量表得分明显较低（p = 0.004, Cohen’s d = 0.46）。然而，其他WHOQOL-BREF量表和抑郁水平无显著差异（p < 0.05）。在乳糜泻组中，高等教育水平（本科和研究生）与更好的生活质量评分相关（η2 = 0.11, p = 0.015），而低教育水平与更高的抑郁评分相关（p = 0.019）。CDQ评分与BDI评分呈显著负相关（r = -0.529, p = 0.001），表明抑郁水平越高，生活质量越低。当控制混杂变量时，性别和乳糜泻被证明与总体健康亚量表得分独立相关。结论：在我们的研究中，CD患者的生活质量量表CDQ和WHOQOL-BREF评分均领先于曲线。研究还发现，乳糜泻患者的WHOQOL-BREF量表“整体健康”亚量表得分较低，而其他子参数和抑郁症状与健康个体相当，较低的“整体健康”亚量表得分突出了有针对性干预的必要性。

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引用次数: 0

Changes of peripheral blood lymphocytes, neutrophils, CEA, TAP and ferritin in colorectal adenoma and colorectal cancer and the diagnostic performance of these makers in evaluating colorectal cancer 外周血淋巴细胞、中性粒细胞、CEA、TAP和铁蛋白在结直肠腺瘤和结直肠癌中的变化及其对结直肠癌的诊断价值

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-05-08 DOI: 10.1016/j.ajg.2025.03.002

Jialai Zheng , Yongtao Tu , Haiyong Jin , Haiyan Sun , Guanqiao Shen , Haijiang Tong

Background and study aims

Colorectal cancer (CRC) has been recognized as a multifactorial disease arising from precursor lesions and characterized by chronic inflammation. Therefore, the inflammatory response is one of the critical indicators for the diagnosis of CRC. Timely and effective screening is an effective strategy to reduce the incidence and mortality of CRC. The primary aim of this study was to analyze the differences in white blood cell count (WBC) in patients with colorectal adenoma (CRA) and CRC. The second aim was to estimate the diagnostic performance of using a panel of serum tumor markers and WBC for CRC screen.

Patients and methods

We retrospectively reviewed a database of patients who were diagnosed with CRA and CRC. Serum tumor markers and blood routine examination data were completed before receiving any anticancer therapy.

Results

A total of 538 participants were enrolled, including 169 health participants, 195 patients with CRA and 174 patients with CRC. Lymphocyte counts were lower in CRC than CRA and healthy participants. Neutrophil counts were higher in CRC and CRA than healthy participants. The CEA levels were higher in CRA and CRC than healthy participants, and higher in CRC than CRA. The areas of tumor TAP were larger in CRC than CRA and healthy participants. The ferritin levels were lower in CRC than CRA and healthy participants. The 8-marker panel yielded an AUC of 0.854 higher than single marker. There is no difference in the diagnostic performance of TAP, CEA, ferritin and NLR.

Conclusions

There are a lot of high sensitivity and specificity methods for CRC screening. However, most screening programs suffer from poor participation rates. Herein, our 8-marker panel is cost-effective and high-performance screen system for the detection of CRC and is crucial for enhancing the participation rates in current screening programs.

背景与研究目的：结直肠癌（Colorectal cancer， CRC）是一种由前体病变引起的多因素疾病，以慢性炎症为特征。因此，炎症反应是诊断结直肠癌的重要指标之一。及时有效的筛查是降低结直肠癌发病率和死亡率的有效策略。本研究的主要目的是分析结直肠腺瘤（CRA）和结直肠癌患者白细胞计数（WBC）的差异。第二个目的是评估使用一组血清肿瘤标志物和白细胞筛查结直肠癌的诊断性能。患者和方法：我们回顾性地回顾了诊断为CRA和CRC的患者数据库。在接受任何抗癌治疗前完成血清肿瘤标志物和血常规检查资料。结果：共纳入538名参与者，包括169名健康参与者，195名CRA患者和174名CRC患者。CRC患者的淋巴细胞计数低于CRA和健康参与者。CRC和CRA患者的中性粒细胞计数高于健康参与者。CRA组和CRC组CEA水平均高于健康组，CRC组CEA水平高于CRA组。CRC患者的肿瘤TAP面积大于CRA和健康参与者。CRC组的铁蛋白水平低于CRA组和健康组。8个标记组的AUC比单个标记高0.854。TAP、CEA、铁蛋白和NLR的诊断效果无显著差异。结论：目前有很多高灵敏度、高特异性的CRC筛查方法。然而，大多数筛查项目的参与率都很低。因此，我们的8个标记面板是一种成本效益高、高性能的CRC检测筛查系统，对于提高当前筛查计划的参与率至关重要。

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引用次数: 0

Comparison of 12- and 15-mm endoscopic papillary large balloon dilation combined with a limited endoscopic sphincterotomy for large bile duct stones: A propensity score-matched analysis 12和15毫米内镜下乳头状大球囊扩张联合有限内镜下括约肌切开术治疗大胆管结石的比较：倾向评分匹配分析。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-05-06 DOI: 10.1016/j.ajg.2025.03.003

Qian Zhang , Lili Chen , Jun Liu , Weiwei Chen , Meng Zhou , Chaowu Chen

Background and study aims

Endoscopic papillary large balloon dilation (EPLBD) combined with endoscopic sphincterotomy (EST) has been increasingly used to remove large common bile duct (CBD) stones. However, the clinical outcomes of stone extraction have rarely been investigated in regard to the size of the balloon. The aim of this study was to assess the short- and long-term outcomes of 12- and 15-mm EPLBD with a limited EST for large CBD stones.

Patients and methods

Patients without prior endoscopic retrograde cholangiopancreatography (ERCP) who received 12- or 15-mm EPLBD in combination with a limited EST for large CBD stones at our center between January 2013 and December 2020 were recruited. Matched pairs with 12- and 15-mm EPLBD were generated.

Results

A total of 161 patients were included, with 50 patients successfully matched in each group. The total procedure time (36.8 min vs. 47.3 min, p = 0.003) and the tone removal time (30.5 min vs. 39.2 min, p = 0.008) were significantly lower in the matched 15-mm EPLBD group, while the initial and overall stone removal success rates were comparable between the two groups (p > 0.05). The matched 15-mm EPLBD group had a lower incidence of post-ERCP pancreatitis (PEP) compared to the matched 12-mm EPLBD group (2.0 % vs. 16.0 %, p = 0.031). The cumulative long-term biliary complications were not statistically different between the two groups (log-rank test p = 0.612).

Conclusion

15-mm EPLBD combined with a limited EST shortened the procedure time and reduced the incidence of PEP without increasing long-term biliary complications compared to 12-mm EPLBD combined with a limited EST.

背景与研究目的：内镜下乳头状大球囊扩张术（EPLBD）联合内镜下括约肌切开术（EST）越来越多地用于大胆总管（CBD）结石的切除。然而，结石取出的临床结果很少与球囊的大小有关。本研究的目的是评估12毫米和15毫米EPLBD与有限EST对大型CBD结石的短期和长期结果。患者和方法：招募了2013年1月至2020年12月期间在本中心接受12或15毫米EPLBD联合有限EST治疗大CBD结石的未经内镜逆行胆管造影（ERCP）的患者。生成了具有12和15 mm EPLBD的配对对。结果：共纳入161例患者，每组成功配对50例。15毫米EPLBD组的总手术时间（36.8分钟vs. 47.3分钟，p = 0.003）和拔牙时间（30.5分钟vs. 39.2分钟，p = 0.008）显著低于匹配的15毫米EPLBD组，而两组之间的初始和总体拔牙成功率相当（p = 0.05）。与匹配的12 mm EPLBD组相比，匹配的15 mm EPLBD组ercp后胰腺炎（PEP）的发生率较低（2.0%比16.0%,p = 0.031）。两组患者累积的长期胆道并发症无统计学差异（log-rank检验p = 0.612）。结论：与12mm EPLBD联合有限EST相比，15mm EPLBD联合有限EST缩短了手术时间，降低了PEP的发生率，且未增加长期胆道并发症。

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引用次数: 0

Cinnamaldehyde attenuates CCL4-induced liver fibrosis by inhibiting the CYP2A6/Notch3 pathway 肉桂醛通过抑制CYP2A6/Notch3通路减轻ccl4诱导的肝纤维化。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-05-05 DOI: 10.1016/j.ajg.2025.04.001

Sicheng Gao , Wanyi Zhang , Xiaodi Gao , Baiyang Ye , Weiye Hu , Hailin Yang , Haisheng Chai , Jiangling Yang , Qinlin Tang , Gang Zhao , Junfeng Zhu

Background

Hepatic stellate cells (HSCs) activation and hepatocyte injury contribute to liver fibrosis progression and subsequent cirrhosis. Literature showed that cinnamaldehyde (CA) could alleviate fibrosis procession and steatosis. However, its specific role in liver fibrosis remains largely unexplored.

Materials and methods

Liver fibrosis was induced in vivo, and CA was administered for 4 weeks. Liver inflammation, fibrosis, apoptosis, and proliferation were evaluated using histological, western blotting, and immunohistochemistry. CYP2A6 and Notch3 expression levels were also measured. In vitro, TGF-β stimulated LX2 cell activation was used, and siCYP2A6 was employed to evaluate the anti-fibrosis mechanism of CA.

Results

CA effectively improved liver function and reduced fibrosis in CCL4-treated rats, significantly decreasing serum ALT, AST, GGT, TBIL, and HAase levels (all p < 0.05), with a notable increase in ALB in the high-dose group. Histologically, CA reduced hepatic disorganization and collagen proliferation, significantly diminishing fibrotic areas in the CA-H group (p < 0.05). CA also downregulated α-SMA and collagen I expression, and suppressed TGF-β activity. In TGF-β1-stimulated LX2 cells, CA treatment led to significant reductions in CYP2A6 and Notch3 expression (p < 0.05), highlighting its regulatory effects on key fibrotic pathways.

Conclusions

CA alleviated CCL₄-induced liver fibrosis with inhibition of HSCs activation and liver inflammation and reduced hepatocyte apoptosis, potentially linked to the HSCs-mediated CYP2A6/Notch3 modulation.

背景：肝星状细胞（HSCs）活化和肝细胞损伤有助于肝纤维化进展和随后的肝硬化。文献表明，肉桂醛（CA）可减轻纤维化过程和脂肪变性。然而，其在肝纤维化中的具体作用在很大程度上仍未被探索。材料与方法：体外诱导肝纤维化，给予CA治疗4周。采用组织学、免疫印迹和免疫组织化学方法评估肝脏炎症、纤维化、凋亡和增殖。同时检测CYP2A6和Notch3的表达水平。体外采用TGF-β刺激LX2细胞活化，并用siCYP2A6评价CA抗纤维化机制。结果：CA能有效改善ccl4治疗大鼠肝功能，减轻纤维化，显著降低血清ALT、AST、GGT、TBIL、HAase水平（均p）。CA通过抑制hsc激活和肝脏炎症以及减少肝细胞凋亡来减轻ccl4诱导的肝纤维化，这可能与hsc介导的CYP2A6/Notch3调节有关。

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引用次数: 0

Abnormal gastroesophageal flap valve: A predictor of recurrent variceal hemorrhage 胃食管瓣瓣异常：复发性静脉曲张出血的预测因子。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-05-01 DOI: 10.1016/j.ajg.2025.01.014

Jay Chudasama , Shubham Jain , Sanjay Chandnani , Anuraag Jena , Harsh Gandhi , Rishikesh Malokar , Sameet Patel , Saurabh Bansal , Pankaj Nawghare , Vishal sharma , Pravin Rathi , Qais Contractor

Background and study aims

Esophageal variceal bleeding is affected by various risk factors. We hypothesized that increased exposure to gastric acid in patients with abnormal gastroesophageal flap valve (GEFV) might increase esophageal variceal bleeding. The aim of this study is to investigate the relationship between GEFV and esophageal variceal bleeding episodes.

Patients and methods

In this cross-sectional study, 300 consecutive patients with esophageal varices and a documented GEFV during esophagogastroduodenoscopy were included. Patients were divided into two groups according to: the Hill’s grade of flap valve (grade 1,2- normal and grade 3,4- abnormal), size of varices − large (>5 mm) and small (<5 mm) and the number of bleeding episodes into: Group A with ≤ 1 and Group B with ≥ 2 bleeding episodes. We compared GEFV and various other factors to the number of variceal bleeding episodes.

Results

224 patients (74.60 %) had a normal and 76 (25.40 %) had an abnormal GEFV. Clinical variables were statistically significant in the abnormal GEFV group (P < 0.0.5). Propensity score matching was done to reduce the significant differences in the clinical background at baseline between the 2 groups. 152 patients (76 in each group) were analysed after propensity score matching. A significant difference between the two groups disappeared except for number of bleeding episodes. Binary logistic Cox regression analysis was applied using the clinical variables to assess their role in predicting recurrent variceal bleeding. On univariate analysis, abnormal GEFV and large varices were significantly associated with recurrent esophageal variceal bleed (P = 0.001). On Multivariate analysis, abnormal GEFV (OR 7.25, 95 % CI 3.27– 16.08, P = 0.001), History of EVL (OR 6.21, 95 % CI 1.98– 12.22, P = 0.03) and large varices (OR 5.70, 95 % CI 2.45– 13.20, P = 0.001) were independent predictors for recurrent esophageal variceal bleeding.

Conclusion

Abnormal GEFV, History of EVL and large varices are independent risk factors for recurrent esophageal variceal haemorrhage.

背景与研究目的：食管静脉曲张出血受多种危险因素影响。我们假设胃食管瓣（GEFV）异常患者胃酸暴露增加可能会增加食管静脉曲张出血。本研究的目的是探讨GEFV与食管静脉曲张出血发作的关系。患者和方法：在这项横断面研究中，纳入了连续300例食管胃十二指肠镜检查中伴有食管静脉曲张和有记录的GEFV的患者。根据皮瓣瓣的Hill分级（1、2级正常，3、4级异常）、静脉曲张大小(大（> ~ 5mm）和小（结果：224例（74.60%）正常，76例（25.40%）异常）分为两组。结论：GEFV异常、EVL病史、大静脉曲张是食管静脉曲张出血复发的独立危险因素。

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引用次数: 0

Identification and verification of a combined ferroptosis- and pyroptosis-related signature for a prognostic classifier and immunosuppressive targets in colorectal cancer 结直肠癌预后分类和免疫抑制靶点的铁下垂和热下垂联合特征的鉴定和验证。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-05-01 DOI: 10.1016/j.ajg.2025.02.001

Xiao Wang, Yanting Hu

Background and study aims

Ferroptosis and pyroptosis, two forms of cell death, are increasingly reported for their pivotal roles in cancer biology. However, the understanding of the combined ferroptosis-pyroptosis (FPtosis)-related gene signature in colorectal cancer (CRC) remains limited.

Material and methods

We conducted a comprehensive investigation of the FPtosis-related signature in CRC. Data integration from both the training and validation cohorts was performed. The FPtosis-related signature was established. We evaluated the prognostic significance of the signature through Kaplan-Meier analysis, as well as univariate and multivariate Cox regression models. Functional analyses were conducted to explore the underlying biological mechanisms. Additionally, we analyzed the correlations between the FPtosis-related signature, immune infiltration, and immune checkpoint blockade (ICB) immunotherapy.

Results

The FPtosis-related signature demonstrated significant prognostic potential and can serve as an independent biomarker for predicting outcomes. The signature showed correlations with advanced tumor stage, invasion depth, lymph node metastasis, and distant metastasis. Subgroup analyses revealed the valuable predictive role of the FPtosis-related signature in predicting survival across different clinical subgroups, including age, gender, tumor stage, invasion depth, lymph node metastasis status, and distant metastasis status. Moreover, the signature exhibited positive associations with inflammation and the infiltration of diverse immune cells, such as neutrophils, M0 and M2 macrophages, and regulatory T cells (Tregs). In microsatellite instable (MSI) CRC, the expression of most ICB genes was higher in the high-FPtosis group compared to the low-FPtosis group.

Conclusion

The FPtosis signature can effectively predict the prognosis of CRC and had the potential to improve the development of innovative therapeutic strategies.

背景与研究目的：铁腐和焦腐是细胞死亡的两种形式，在癌症生物学中起着关键作用，近年来越来越多的报道报道。然而，对结直肠癌（CRC）中嗜铁-焦亡（FPtosis）联合相关基因特征的了解仍然有限。材料和方法：我们对CRC中fptosis相关的签名进行了全面的调查。对来自训练组和验证组的数据进行整合。建立了fptosis相关签名。我们通过Kaplan-Meier分析以及单变量和多变量Cox回归模型评估了该特征的预后意义。进行功能分析以探索潜在的生物学机制。此外，我们分析了fptosis相关特征、免疫浸润和免疫检查点阻断（ICB）免疫治疗之间的相关性。结果：fptosis相关特征显示出显著的预后潜力，可以作为预测预后的独立生物标志物。该特征与肿瘤分期、浸润深度、淋巴结转移和远处转移有关。亚组分析显示fptosis相关特征在预测不同临床亚组的生存方面具有重要的预测作用，包括年龄、性别、肿瘤分期、侵袭深度、淋巴结转移状态和远处转移状态。此外，该特征与炎症和多种免疫细胞（如中性粒细胞、M0和M2巨噬细胞以及调节性T细胞（Tregs））的浸润呈正相关。在微卫星不稳定（MSI） CRC中，大多数ICB基因在高fptosis组的表达高于低fptosis组。结论：FPtosis标记可以有效预测结直肠癌的预后，并具有促进创新治疗策略发展的潜力。

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引用次数: 0

Clinical efficacy of phenobarbital combined with ursodeoxycholic acid in the treatment of neonatal patients with cholestasis 苯巴比妥联合熊脱氧胆酸治疗新生儿胆汁淤积症的临床疗效观察。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-05-01 DOI: 10.1016/j.ajg.2024.12.005

Lin Jiang, Qiuyu Jiang, Can Yang

Background and study aims

The aim of this study was to explore and evaluate the clinical effect of phenobarbital combined with ursodeoxycholic acid in the treatment of neonatal cholestasis.

Patients and methods

100 infants were divided into two groups using the random number method, with 50 cases in each group. The PB group was given routine treatment and phenobarbital, and the combination group received the same treatment as the PB group and was additionally given ursodeoxycholic acid. Observe the liver function indicators (γ-glutaminyl transferase (γ-GGT), aspartate transferase (AST), and alanine aminotransferase (ALT)), serum bilirubin (the total bilirubin (TBIL), and direct bilirubin (DBIL)), total effective rate and adverse reactions of the children.

Results

After treatment, the levels of γ-GGT, AST, ALT DBIL, and TBIL in both groups of children were reduced (P < 0.01). the jaundice index of children in the combination group was clearly reduced (P < 0.05) and was obviously lower than that of the PB group (P < 0.05). Moreover, children in the combination group had more daily defecation times than the PB group, and the time to first discharge and yellowing of meconium was shorter than that in the PB group (P < 0. 05). The total effective rate of treatment in the combination group was obviously higher than that in the PB group (94.00 % VS. 78.00 %, P < 0.05), and the average time for complete resolution of jaundice also was shorter than that in the PB group (P < 0.05). A few children develop diarrhea, which occasionally causes skin itching, rash, and other adverse reactions, but most of them are mild and will not affect the treatment effect or prognosis.

Conclusion

Phenobarbital combined with ursodeoxycholic acid can improve the liver function of children to a greater extent in the treatment of neonatal cholestasis. It can also reduce serum bilirubin concentration, has good clinical efficacy, does not cause serious adverse reactions, and has good safety.

背景与研究目的：探讨并评价苯巴比妥联合熊去氧胆酸治疗新生儿胆汁淤积症的临床疗效。患者与方法：采用随机数字法将100例患儿分为两组，每组50例。PB组给予常规治疗和苯巴比妥治疗，联合用药组与PB组治疗相同，加用熊去氧胆酸治疗。观察患儿肝功能指标（γ-谷氨酰基转移酶（γ-GGT）、天冬氨酸转移酶（AST）、丙氨酸转氨酶（ALT））、血清胆红素（总胆红素（TBIL）、直接胆红素（DBIL））、总有效率及不良反应。结果：治疗后两组患儿γ-GGT、AST、ALT、DBIL、TBIL水平均降低(P)。结论：苯巴比妥联合熊去氧胆酸治疗新生儿胆汁淤积症能更大程度地改善患儿肝功能。还能降低血清胆红素浓度，临床疗效好，不发生严重不良反应，安全性好。

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引用次数: 0

Retraction notice to “miR-129-2-3p inhibits colon cancer cell proliferation by down-regulating the expression of BZW1” [Arab J. Gastroenterol. 25 (2024) 42−50] “miR-129-2-3p通过下调BZW1表达抑制结肠癌细胞增殖”的撤回通知[j]. Gastroenterol. 25(2024) 42-50。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-05-01 DOI: 10.1016/j.ajg.2025.03.001

Liang Tao, Peng Song, Lihua Shao, Hengfei Gao, Kangkang Ji, Yan Ren, Feng Wang, Meng Wang

引用次数: 0

Establishing consensus on Arabic medical terminology for steatotic liver disease: a mixed-methods approach 建立对脂肪变性肝病阿拉伯医学术语的共识：混合方法方法。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-05-01 DOI: 10.1016/j.ajg.2025.01.009

Mohamed El-Kassas , Khalid Al-Naamani , Mohamed Elbadry , Abeer Awad , Mina Tharwat , Nabil Debzi , Shahrazed Zemmouchi , Maheeba Abdulla , Doaa Zakaria , Gamal Esmat , Hanaa El-Karaksy , Imam Waked , Inass Shaltout , Mohammed A. Medhat , Mortada El-Shabrawi , Nermeen Abdeen , Mudher Al-khairalla , Maisam W. Akroush , Ali A. Alali , Maen Almattooq , Khalid Alswat

引用次数: 0

Mesenchymal stem cell derived exosomes as Nanodrug carrier of doxorubicin combined with PVT1 siRNA inhibits the progression of gastric cancer 间充质干细胞外泌体作为纳米药物载体联合PVT1 siRNA抑制胃癌的进展。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-05-01 DOI: 10.1016/j.ajg.2025.01.012

Deliang Ouyang, Haibin Li, Kang Luo, Mingming Huang, Song Yan, Li Zhou

Background and study aims

Mesenchymal stem cell-derived exosomes (MSC-Exos) have been used as drug delivery vehicles for the treatment of gastric cancer. This study aimed to explore the effects of doxorubicin-loaded exosomes (Exo-Dox) combined with the long noncoding RNA PVT1 on gastric cancer (GC) development.

Material and methods

CCK-8 and immunohistochemistry were used to assess cell proliferation. The morphology and size of the exosomes and Exo-Dox were determined. The distribution of free Exos and Exo-Dox in cells was observed under a fluorescence microscope. Cell migration and invasive ability were assessed using wound healing and Transwell assays. In addition, the protective effects of Exo-Dox were confirmed in a xenograft tumor model.

Results

Exosomes were successfully isolated from MSCs and identified. The size of Exo-Dox was greater than that of free Exos. The acidic environment promoted the release of doxorubicin, and exosomes promoted the cellular uptake of doxorubicin. Compared with doxorubicin alone, Exo-Dox exhibited better antitumor effects on gastric cancer, inhibiting the growth, migration and invasion of gastric cancer cells. Additionally, combined therapy of Exo-Dox with si-PVT1 clearly suppressed the proliferation, migration and invasive ability of gastric cancer cells. Exo-Dox combined with si-PVT1 inhibited tumor growth and metastasis in a xenograft model.

Conclusion

Doxorubicin-loaded exosomes combined with si-PVT1 suppressed the progression of GC.

背景与研究目的：间充质干细胞衍生外泌体（MSC-Exos）已被用作治疗胃癌的药物递送载体。本研究旨在探讨负载阿霉素的外泌体（Exo-Dox）联合长链非编码RNA PVT1对胃癌（GC）发展的影响。材料与方法：采用CCK-8和免疫组织化学方法评价细胞增殖。测定外泌体和Exo-Dox的形态和大小。荧光显微镜下观察游离Exos和Exo-Dox在细胞内的分布。采用伤口愈合和Transwell试验评估细胞迁移和侵袭能力。此外，Exo-Dox的保护作用在异种移植肿瘤模型中得到证实。结果：成功分离并鉴定了MSCs外泌体。Exo-Dox的大小大于游离Exos。酸性环境促进阿霉素的释放，外泌体促进阿霉素的细胞摄取。与单独使用阿霉素相比，Exo-Dox对胃癌表现出更好的抗肿瘤作用，抑制胃癌细胞的生长、迁移和侵袭。此外，Exo-Dox与si-PVT1联合治疗可明显抑制胃癌细胞的增殖、迁移和侵袭能力。Exo-Dox联合si-PVT1在异种移植模型中抑制肿瘤生长和转移。结论：负载阿霉素的外泌体联合si-PVT1可抑制胃癌的进展。

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引用次数: 0