Arab Journal of Gastroenterology最新文献

英文中文

Molecular subtypes based on the expression of glutamine metabolism-related genes predict prognosis, tumor microenvironment, and drug therapy response of colon adenocarcinoma 基于谷氨酰胺代谢相关基因表达的分子亚型预测结肠腺癌的预后、肿瘤微环境和药物治疗反应。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 Epub Date: 2025-09-21 DOI: 10.1016/j.ajg.2025.08.006

Lijian Yu , Xia Lin

Background and study aims

Glutamine metabolism (GM) plays an instrumental role in the occurrence and progression of tumors. However, the characteristics of glutamine metabolism-related genes (GMRGs) in colon adenocarcinoma (COAD) have not been elucidated. This project aims to dig out the role of GMRGs in COAD molecular subtypes, prognosis, and tumor microenvironment (TME) to proffer a scientific foundation for the evaluation of COAD prognosis and mechanism research.

Material and methods

Disease data of COAD (including clinical information and gene expression data) and GMRG data were collected from The Cancer Genome Atlas (TCGA)-COAD. GMRGs in COAD were obtained through differential analysis, cluster analysis, protein–protein interaction (PPI) network analysis, and regression analysis for setting up a prognostic model. Disease information was collected from GSE29621 to validate the model. Differences in immunotherapy response and drug sensitivity among high-Riskscore (HR) and low-Riskscore (LR) groups were evaluated.

Results

45 GMRGs associated with COAD survival rate were identified through univariate analysis. Cluster and survival analyses revealed that these genes could cluster the COAD population into two clusters with great survival differences. A risk model with 10 feature genes was established through regression analysis. Prediction of immunotherapy sensitivity uncovered that the LR group was more sensitive to immunotherapy.

Conclusion

In summary, this project set up a reliable prognostic risk model with GMRGs, which can proffer a feasible basis for the study of prognosis and related mechanisms in COAD.

背景与研究目的：谷氨酰胺代谢（Glutamine metabolism， GM）在肿瘤的发生发展中起重要作用。然而，谷氨酰胺代谢相关基因（GMRGs）在结肠腺癌（COAD）中的特征尚未阐明。本项目旨在挖掘GMRGs在COAD分子亚型、预后及肿瘤微环境（tumor microenvironment， TME）中的作用，为COAD预后评价及机制研究提供科学依据。材料与方法：从The Cancer Genome Atlas (TCGA)-COAD收集COAD的疾病数据（包括临床信息和基因表达数据）和GMRG数据。通过差异分析、聚类分析、蛋白-蛋白相互作用（PPI）网络分析和回归分析获得COAD的GMRGs，建立预后模型。从GSE29621中收集疾病信息以验证模型。评估高风险评分组（HR）和低风险评分组（LR）免疫治疗反应和药物敏感性的差异。结果：通过单因素分析，鉴定出45个与COAD生存率相关的GMRGs。聚类和生存分析表明，这些基因可以将COAD群体聚为两个生存差异较大的聚类。通过回归分析，建立了包含10个特征基因的风险模型。免疫治疗敏感性预测显示LR组对免疫治疗更敏感。结论：综上所述，本项目建立了可靠的GMRGs预后风险模型，可为COAD预后及相关机制的研究提供可行性依据。

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引用次数: 0

Corrigendum to “Paediatric autoimmune liver diseases: A descriptive study of patients from Saudi Arabia”. [Arab J. Gastroenterol. 22(2) (2021) 146–150] “儿童自身免疫性肝病：对沙特阿拉伯患者的描述性研究”的勘误表。[j].胃肠病杂志，22(2)(2021)146-150。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 Epub Date: 2025-11-07 DOI: 10.1016/j.ajg.2025.08.009

Omar I. Saadah , Ammar Khayat , Hadeel A. Alsufyani , Yagoub Bin-Taleb

引用次数: 0

Helicobacter pylori and Porphyromonas gingivalis as risk factors in the enteric metaplasia–dysplasia–oncogenesis sequence 幽门螺杆菌和牙龈卟啉单胞菌是肠化-发育不良-肿瘤发生顺序的危险因素。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 Epub Date: 2025-09-24 DOI: 10.1016/j.ajg.2025.07.001

Jannis Kountouras , Christos Zavos , Stergios A. Polyzos , Maria Tzitiridou-Chatzopoulou , Paraskevi Polyzou-Laspia , Dimitrios Chatzopoulos , Michael Doulberis , Elisabeth Vardaka , Ioannis S. Papanikolaou

引用次数: 0

Comparative effects of pirfenidone and nintedanib in experimental intraabdominal adhesion model 吡非尼酮与尼达尼布对实验性腹腔粘连模型的影响比较。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 Epub Date: 2025-09-04 DOI: 10.1016/j.ajg.2025.07.002

Olgun Erdem , Selin Karaman Erdem , Abdullah Yıldız , Hakan Çakıt , Ceren Canbey , Ömer Faruk Özkan

Background

Postoperative peritoneal adhesions (PPA) develop in up to 90% of intraabdominal surgeries and are a major cause of small bowel obstruction, leading to readmissions and morbidity. However, no effective pharmacologic strategy currently exists for PPA prevention.

Pirfenidone and Nintedanib are oral antifibrotics approved for idiopathic pulmonary fibrosis, with emerging data on their effects in cardiac and hepatic fibrosis. This study aimed to compare their efficacy in preventing PPA via intraperitoneal (IP) administration in an experimental model.

Methods

Twenty-eight rats were randomized into four groups of 7: Pirfenidone (P), Nintedanib (N), combination (P + N), and saline control (C). Adhesions were induced via cecal abrasion. On postoperative day 7, macroscopic fibrosis scores (MFS), affected regions, histopathology, and TGF-β1 expression were evaluated.

Results

Group P had significantly lower MFS than Group N (P < 0.001) and showed a reduction trend vs. control. TGF-β1 HScore was significantly lower in Group P vs. Group N (P = 0.002) and P + N (P = 0.014). Group N showed the highest inflammation and elevated TGF-β1 vs. control (P = 0.013).

Conclusion

Pirfenidone appears effective in reducing PPA formation via IP administration. In contrast, Nintedanib may be unsuitable for IP use in saline, potentially due to poor solubility.

背景：术后腹膜粘连（PPA）在高达90%的腹内手术中发生，是小肠梗阻的主要原因，导致再入院和发病率。然而，目前尚无有效的药理学策略来预防PPA。吡非尼酮和尼达尼布是批准用于特发性肺纤维化的口服抗纤维化药物，其在心脏和肝纤维化中的作用正在出现新的数据。本研究的目的是在实验模型中比较它们通过腹腔注射（IP）预防PPA的功效。方法：将28只大鼠随机分为吡非尼酮(P)、尼达尼布(N)、联合用药（P + N）和生理盐水对照组(C) 4组，每组7只。通过盲肠磨损诱导粘连。术后第7天观察宏观纤维化评分（MFS）、影响区域、组织病理学及TGF-β1表达情况。结果：P组患者MFS明显低于N组。结论：吡非尼酮通过给药可有效减少PPA的形成。相反，尼达尼布可能不适合在生理盐水中使用IP，可能是由于溶解性差。

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引用次数: 0

Cholecystohepatic duct (type IIIA) – fifth report of aberrant biliary pathology in a paediatric case of oesophageal atresia and imperforate anus with review of literature 胆囊肝管（IIIA型）：第5例食道闭锁和肛门闭锁的小儿胆道异常病理报告并复习文献。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 Epub Date: 2025-09-20 DOI: 10.1016/j.ajg.2025.07.004

Sushma Achugatla , Rajeev Redkar , Surendra Singh , Sonia Thakur , Jui Mandke

It is known that the presence of associated anomalies has become one of the most critical prognostic factor for infants born with oesophageal atresia (OA). Associated VACTERAL group of abnormalities are common, but other associations are rare. Anomalies of the hepatobiliary system are even more rare among other groups. There are only a few such cases in the literature including adults and paediatric population. We describe the findings and management of one infant born with OA with tracheoesophageal fistula (TEF) and Ano rectal malformation (ARM), who was later shown to have features of obstructive jaundice. The biliary pathology was due to a preoperative diagnosis of Type 1c choledochal cyst, but in reality it was associated with an aberrant form of biliary drainage in the form of cholecystohepatic duct (CCHD). In the English literature, there are only four reported cases of cholecystohepatic duct (CCHD) in children associated with complex congenital anomalies [[1], [2], [3]]. The association of these multiple complex congenital anomalies with aberrant biliary duct is a rare feature. Here, in this case, this aberrant cholecystohepatic duct is the only drainage pathway of bile from liver into gallbladder

众所周知，相关异常的存在已成为食道闭锁（OA）婴儿最关键的预后因素之一。相关的空腔组异常是常见的，但其他的相关是罕见的。肝胆系统异常在其他人群中更为罕见。在包括成人和儿童在内的文献中，只有少数这样的病例。我们描述了一名出生时患有OA并伴有气管食管瘘（TEF）和肛门直肠畸形（ARM）的婴儿的发现和治疗，该婴儿后来被证明具有阻塞性黄疸的特征。胆道病理是由于术前诊断为1c型胆总管囊肿，但实际上它与胆囊肝管（CCHD）形式的异常胆道引流有关。在英文文献中，仅报道了4例伴有复杂先天性异常的儿童胆囊肝管（CCHD）[1-3]。这些多发性复杂先天性异常与胆管异常的关联是一种罕见的特征。在这个病例中，这个异常的胆囊-肝管是胆汁从肝脏进入胆囊的唯一引流途径。

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引用次数: 0

A case report of primary amelanotic malignant melanoma of esophagus 食管原发性无黑色素瘤恶性黑色素瘤1例。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 Epub Date: 2025-09-20 DOI: 10.1016/j.ajg.2025.08.007

Qiqi Ma , Shanbin Wu , Yuting Yang , Sitong Guo , Mei Han , Yan Yan

Primary malignant melanoma of the esophagus (PMME) is an extremely rare malignant tumor. Primary amelanotic malignant melanoma is a subtype of PMME which shows no pigmentation under esophagogastroduodenoscopy (EGD). A lack of understanding of this disease makes it susceptible to misdiagnosis and limits its treatment options. Here, we reported a case which misdiagnosed as esophageal polyps with malignant transformation or esophageal cancer when observed on gastroscopy examination, while proved as primary amelanotic malignant melanoma by immunohistochemistry in our department. The aim of this study is to draw the attention of physicians by describing a case of primary amelanotic malignant melanoma and to aid in the diagnosis of this disease in the future.

原发性食道恶性黑色素瘤是一种极为罕见的恶性肿瘤。原发性无色素变性恶性黑色素瘤是PMME的一种亚型，在食管胃十二指肠镜（EGD）下无色素沉着。对这种疾病缺乏了解使其容易误诊并限制其治疗选择。我们报告了一例胃镜检查误诊为食管息肉伴恶性转化或食管癌，而经免疫组化证实为原发性无色素变性恶性黑色素瘤的病例。本研究的目的是通过描述一个原发性无黑色素瘤恶性黑色素瘤的病例来引起医生的注意，并有助于将来对这种疾病的诊断。

引用次数: 0

A Systematic analysis of gut microbiota in anorectal abscess and anal Fistula, with the mediating role of immune cells 肠道菌群在肛肠脓肿和肛瘘中的系统分析及免疫细胞的介导作用。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 Epub Date: 2025-11-05 DOI: 10.1016/j.ajg.2025.09.004

Pan Shen , Qinghua Luo , Yifan Ding , Hao Xiong , Leichang Zhang

Background and study aims

This study aimed to investigate the causal relationships between gut microbiota, immune cells, and the development of anorectal abscess and anal fistula through Mendelian randomization (MR) analysis. The objective was to identify gut microbial taxa and immune cell phenotypes that contribute to disease pathogenesis, thereby providing new insights into the underlying mechanisms.

Materials and methods

Two-sample MR analysis was conducted using genetic data from large-scale genome-wide association studies (GWAS), encompassing 211 gut microbiota taxa and 731 immune cell phenotypes. The inverse variance weighted (IVW) method served as the primary analysis, complemented by sensitivity analyses such as MR-Egger, MR-PRESSO, and leave-one-out tests. Mediation MR analysis was employed to assess the role of immune cells as mediators.

Results

Ten gut microbiota taxa were significantly associated with anorectal abscess, while six taxa were linked to anal fistula. Protective taxa included Butyrivibrio, Ruminiclostridium 5, and Sutterella, while Porphyromonadaceae, Actinomyces, and Faecalibacterium were identified as risk factors. Mediation analysis revealed 16 immune cell phenotypes involved in the gut microbiota-disease pathway. Specifically, CD45 on CD33- HLA DR- mediated 17.28 % of the effect of Actinomyces on anorectal abscess, while CD4 on HLA DR + CD4 + mediated −18.26 % of the protective effect of Butyrivibrio on anorectal abscess.

Conclusion

To the best of our knowledge, this study represents the first comprehensive evaluation of causal relationships among gut microbiota, immune cell phenotypes, and anorectal diseases. The findings underscore specific gut microbiota and immune cell phenotypes as critical mediators in disease pathogenesis, offering promising targets for therapeutic intervention and precision treatment strategies.

背景与研究目的：本研究旨在通过孟德尔随机化（Mendelian randomization， MR）分析，探讨肠道菌群、免疫细胞与肛肠脓肿和肛瘘发生的因果关系。目的是确定肠道微生物分类群和免疫细胞表型，有助于疾病发病机制，从而为潜在机制提供新的见解。材料和方法：使用大规模全基因组关联研究（GWAS）的遗传数据进行双样本MR分析，包括211个肠道微生物群分类群和731个免疫细胞表型。反方差加权（IVW）法作为主要分析方法，辅以敏感性分析，如MR-Egger、MR-PRESSO和留一检验。采用中介MR分析来评估免疫细胞作为中介的作用。结果：10个肠道微生物群与肛肠脓肿相关，6个肠道微生物群与肛瘘相关。保护类群包括丁酸弧菌、反刍杆菌5和苏氏菌，而危险类群包括卟啉单胞菌科、放线菌科和粪杆菌。中介分析揭示了16种免疫细胞表型参与肠道微生物群-疾病途径。其中CD45对CD33- HLA DR-介导的放线菌对肛肠脓肿的保护作用为17.28%，而CD4对HLA DR + CD4 +介导的丁酸弧菌对肛肠脓肿的保护作用为- 18.26%。结论：据我们所知，这项研究首次全面评估了肠道微生物群、免疫细胞表型和肛肠疾病之间的因果关系。这些发现强调了特定的肠道微生物群和免疫细胞表型是疾病发病机制的关键介质，为治疗干预和精确治疗策略提供了有希望的靶点。

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引用次数: 0

Hematochezia due to Meckel’s diverticulum in a young male 年轻男性梅克尔憩室所致便血。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 Epub Date: 2025-09-22 DOI: 10.1016/j.ajg.2025.08.002

Cong Gao , Shengye Yang , Zhendong Liang , Chun Ye , Xingshun Qi

引用次数: 0

Investigating the process of apoptosis in HepG2 cells treated with palmitate, oleate, and palmitate/oleate combination 研究棕榈酸酯、油酸酯和棕榈酸酯/油酸酯联合处理HepG2细胞的凋亡过程。

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 Epub Date: 2025-11-06 DOI: 10.1016/j.ajg.2025.09.002

Samaneh Torabi , Yaser Mohammadi , Motahareh-sadat Hoseini , Atena Rezaei , Ali Mohammadi , Elham Bahreini

Introduction

Non-alcoholic fatty liver disease (NAFLD), the most common chronic liver disease, is characterized by lipid droplet (LD) accumulation in more than 5% of hepatocytes. This study aimed to investigate apoptosis in HepG2 cells exposed to palmitate, oleate, and a combination of palmitate and oleate.

Methods

The effective concentrations of palmitic and oleic acids were determined using the MTT assay. Subsequently, an optimal combined dose of these fatty acids was selected to induce LD formation. Intracellular LD accumulation following treatment with different concentrations of palmitate and oleate was assessed qualitatively and semi-quantitatively using Oil Red O staining and light microscopy. Expression levels of apoptosis-related genes (p53, Bax, FasL, and Bcl-2) were quantified using real-time PCR.

Results

Palmitate treatment markedly reduced cell density and enhanced LD accumulation, whereas oleate exhibited milder effects. The palmitate–oleate (P&O) combination induced greater LD accumulation than oleate alone but was less cytotoxic than palmitate. At the molecular level, pro-apoptotic genes (P53, Bax, and FasL) were upregulated in all treated groups, with the highest expression in the P&O group. Conversely, the anti-apoptotic gene Bcl-2 was significantly downregulated in palmitate-treated cells.

Conclusion

Saturated and unsaturated fatty acids exert distinct yet interconnected effects on lipid homeostasis and hepatocyte viability. Maintaining their proper balance may be critical in preventing liver injury. Further studies are warranted to elucidate the underlying mechanisms.

简介：非酒精性脂肪性肝病（NAFLD）是最常见的慢性肝病，其特征是脂滴（LD）在5%以上的肝细胞中积聚。本研究旨在探讨棕榈酸盐、油酸盐以及棕榈酸盐和油酸盐联合作用下HepG2细胞的凋亡情况。方法：采用MTT法测定棕榈酸和油酸的有效浓度。随后，选择这些脂肪酸的最佳组合剂量来诱导LD的形成。使用Oil Red O染色和光学显微镜对不同浓度棕榈酸酯和油酸酯处理后的细胞内LD积累进行定性和半定量评估。实时荧光定量PCR检测凋亡相关基因（p53、Bax、FasL、Bcl-2）的表达水平。结果：棕榈酸盐处理显著降低细胞密度，增加LD积累，而油酸盐处理效果较温和。棕榈酸酯-油酸酯（P&O）组合比单独油酸酯诱导更多的LD积累，但比棕榈酸酯的细胞毒性小。在分子水平上，促凋亡基因（P53、Bax和FasL）在所有处理组中均上调，其中P&O组表达量最高。相反，抗凋亡基因Bcl-2在棕榈酸处理的细胞中显著下调。结论：饱和脂肪酸和不饱和脂肪酸对脂质稳态和肝细胞活力有不同但相互关联的影响。保持它们的适当平衡可能是预防肝损伤的关键。需要进一步的研究来阐明潜在的机制。

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引用次数: 0

Factors associated with inflammatory bowel disease control among IBD patients in Palestine (2023–2024): A cross-sectional study 巴勒斯坦IBD患者炎症性肠病控制相关因素（2023-2024）：一项横断面研究

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology

Pub Date : 2025-11-01 Epub Date: 2025-11-07 DOI: 10.1016/j.ajg.2025.09.012

Qusay Abdoh , Fatima Al-Amouri , Sinyal Kittaneh , Julia Daoud , Saif Hamouda , Manal Badrasawi

Background

Inflammatory bowel disease (IBD) is a chronic inflammatory condition that impacts many aspects of patients’ lives and requires proper control to improve health outcomes. This study aimed to assess perceived IBD control and identify factors associated with it.

Methods

An interview-based questionnaire was used to collect data related to sociodemographic, lifestyle, IBD-related information, and anthropometrics among 116 IBD patients. The inflammatory bowel disease control questionnaire was used to evaluate IBD control, the general health questionnaire-12 (GHQ-12) was used to assess general psychological distress, and the malnutrition universal screening tool (MUST) was used for malnutrition screening.

Results

The mean age of the study participants was 36.4 ± 13.8 years, the mean time since being diagnosed with IBD was 6.2 ± 6.6 years, and the mean IBD-control score was 10.8 ± 4.8. Better IBD control (p < 0.05) was significantly associated with living with a spouse, having a higher educational level, being a non-current smoker, not having sleeping problems, not having changes in sleep duration after being diagnosed, being more physically active or having the same level of physical activity compared to before diagnosis, and being well-nourished. In addition, longer disease duration, higher midarm, calf, and hip circumferences, increased muscle mass, handgrip strength, peak flow rate, and GHQ score were all significantly correlated with IBD control score. In multivariate analysis only the GHQ-12 score and the principal component representing muscle mass and body size were found to be statistically significant.

Conclusions

Psychological well-being and favorable body composition were independently associated with better perceived disease control among individuals with IBD. These findings necessitate the importance of psychological screening and nutritional or functional assessments into IBD management to enhance self-management and improve patients’ overall disease experience.

背景：炎症性肠病（IBD）是一种慢性炎症性疾病，影响患者生活的许多方面，需要适当控制以改善健康结果。本研究旨在评估感知IBD控制并确定与之相关的因素。方法：采用访谈式问卷收集116例IBD患者的社会人口学、生活方式、IBD相关信息和人体测量学数据。采用炎症性肠病控制问卷评估IBD控制情况，采用一般健康问卷-12 （GHQ-12）评估一般心理困扰，采用营养不良普遍筛查工具（MUST）进行营养不良筛查。结果：研究参与者的平均年龄为36.4±13.8岁，平均自诊断为IBD的时间为6.2±6.6年，平均IBD控制评分为10.8±4.8。结论：心理健康和良好的身体组成与IBD患者更好的疾病控制感知独立相关。这些发现表明心理筛查和营养或功能评估对IBD管理的重要性，以增强自我管理和改善患者的整体疾病体验。

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