Pub Date : 2003-09-30DOI: 10.12702/1984-7033.V03N03A05
H. A. Mendonça, J. B. Santos, M. Antonio, P. Ramalho
The Carioca MG (susceptible = P 1 ) and Perola (resistant = P 2 ) cultivars were used as parents in a cross aiming at investigating the genetic control of the common bean plant reaction to Phaeoisariopsis griseola. Two hundred and fifty-one families (141 F 2:3 , 53 F 2 RC 11 and 57 RC 12 ) from this cross were evaluated for angular leaf spot severity in the leaves and pods, in two generations, under natural incidence of the pathogen. The genetic variance components, heritability in the broad ( 2 h ) and narrow ( 2 h ) senses and number of genes that control the reaction to angular leaf spot were estimated. The families were genetically different for level of resistance to the pathogen, and varied from 59.20% to 72.41% and varied from 19.32% to 73.79% for the leaf analysis. For reaction to the pathogen in the pods, the varied from 55.00% to 68.22% and was nil, indicating the presence of dominant alleles in the control of the trait, and of genes different from those responsible for the control of the disease in the leaves. Only one gene was estimated for the genetic control of the reaction to the pathogen in the leaves and two genes in the pods.
{"title":"Genetic control of common bean reaction to angular leaf spot","authors":"H. A. Mendonça, J. B. Santos, M. Antonio, P. Ramalho","doi":"10.12702/1984-7033.V03N03A05","DOIUrl":"https://doi.org/10.12702/1984-7033.V03N03A05","url":null,"abstract":"The Carioca MG (susceptible = P 1 ) and Perola (resistant = P 2 ) cultivars were used as parents in a cross aiming at investigating the genetic control of the common bean plant reaction to Phaeoisariopsis griseola. Two hundred and fifty-one families (141 F 2:3 , 53 F 2 RC 11 and 57 RC 12 ) from this cross were evaluated for angular leaf spot severity in the leaves and pods, in two generations, under natural incidence of the pathogen. The genetic variance components, heritability in the broad ( 2 h ) and narrow ( 2 h ) senses and number of genes that control the reaction to angular leaf spot were estimated. The families were genetically different for level of resistance to the pathogen, and varied from 59.20% to 72.41% and varied from 19.32% to 73.79% for the leaf analysis. For reaction to the pathogen in the pods, the varied from 55.00% to 68.22% and was nil, indicating the presence of dominant alleles in the control of the trait, and of genes different from those responsible for the control of the disease in the leaves. Only one gene was estimated for the genetic control of the reaction to the pathogen in the leaves and two genes in the pods.","PeriodicalId":49085,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"25 1","pages":"209-216"},"PeriodicalIF":1.5,"publicationDate":"2003-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78832288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-09-30DOI: 10.12702/1984-7033.V03N03A08
P. Souza, Joao Gomes Costa
The objective of this study was to estimate the repeatability coefficient, the minimum number of evaluations to which a trait should be subjected and the effect of the inter-relationships of five characters on grapevine (Vitis spp.) yield, aiming at getting useful information for breeding strategies of this fruit crop. Repeatability coefficients were estimated for the following characters: total soluble solids (TSS); total titrable acidity (TTA); TSS/TTA ratio; berry length, diameter and weight; bunch length, width and weight, and number and yield of bunches per plant on eleven seedless grape varieties in five yield cycles (1997 and 1998) in Petrolina-PE. The repeatability estimates were obtained by the main components method from the correlation matrix. The simple correlation coefficients were calculated and they were partitioned into direct and indirect effects by the path analysis. The estimated repeatability coefficients ranged from 0.4750 to 0.8372 associated to the coefficients of determination from 81.9% to 96.26%. The traits TSS, TTA and bunch length presented low repeatability coefficients, meaning a low regularity of performance from one measurement to another. The other characters showed regularity on the repetition of the behavior of the genotypes. The results of the path analysis showed that the studied variables satisfactorily explain the character yield per plant.
{"title":"Estimates of repeatability and path coefficients on grapes","authors":"P. Souza, Joao Gomes Costa","doi":"10.12702/1984-7033.V03N03A08","DOIUrl":"https://doi.org/10.12702/1984-7033.V03N03A08","url":null,"abstract":"The objective of this study was to estimate the repeatability coefficient, the minimum number of evaluations to which a trait should be subjected and the effect of the inter-relationships of five characters on grapevine (Vitis spp.) yield, aiming at getting useful information for breeding strategies of this fruit crop. Repeatability coefficients were estimated for the following characters: total soluble solids (TSS); total titrable acidity (TTA); TSS/TTA ratio; berry length, diameter and weight; bunch length, width and weight, and number and yield of bunches per plant on eleven seedless grape varieties in five yield cycles (1997 and 1998) in Petrolina-PE. The repeatability estimates were obtained by the main components method from the correlation matrix. The simple correlation coefficients were calculated and they were partitioned into direct and indirect effects by the path analysis. The estimated repeatability coefficients ranged from 0.4750 to 0.8372 associated to the coefficients of determination from 81.9% to 96.26%. The traits TSS, TTA and bunch length presented low repeatability coefficients, meaning a low regularity of performance from one measurement to another. The other characters showed regularity on the repetition of the behavior of the genotypes. The results of the path analysis showed that the studied variables satisfactorily explain the character yield per plant.","PeriodicalId":49085,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"20 1","pages":"231-236"},"PeriodicalIF":1.5,"publicationDate":"2003-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77108074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-09-30DOI: 10.12702/1984-7033.V03N03A01
L. R. F. Rodrigues, N. D. Silva, É. Mori
Two experiments were carried out in order to evaluate the effect of plant density on baby corn yield. The traits evaluated were: plant height, ear height, number of ears/plot, weight, length, and diameter of husked and dehusked ears of 21 hybrids, 13 inbred lines, and one commercial control. The experiments were set up in a randomized complete block design with three replicates. The experimental plots consisted of rows with 25 and 50 plants, 5-m long, 0.90 m apart, in densities 1 and 2, respectively. The analysis of variance showed that plant densities significantly affected all traits (P<0.05), except for plant height and weight of husked ears. The hybrid 27Ax31B had the best performance in the density of 55,000 plants/ha, for husked and dehusked ears yield, while the 27Ax29B hybrid was better under 110,000 plants/ha, taking the same traits into consideration. The hybrids did not overcome the husked and dehusked ears yield of inbred 27A in both plant densities.
{"title":"Baby corn single-cross hybrids yield in two plant densities","authors":"L. R. F. Rodrigues, N. D. Silva, É. Mori","doi":"10.12702/1984-7033.V03N03A01","DOIUrl":"https://doi.org/10.12702/1984-7033.V03N03A01","url":null,"abstract":"Two experiments were carried out in order to evaluate the effect of plant density on baby corn yield. The traits evaluated were: plant height, ear height, number of ears/plot, weight, length, and diameter of husked and dehusked ears of 21 hybrids, 13 inbred lines, and one commercial control. The experiments were set up in a randomized complete block design with three replicates. The experimental plots consisted of rows with 25 and 50 plants, 5-m long, 0.90 m apart, in densities 1 and 2, respectively. The analysis of variance showed that plant densities significantly affected all traits (P<0.05), except for plant height and weight of husked ears. The hybrid 27Ax31B had the best performance in the density of 55,000 plants/ha, for husked and dehusked ears yield, while the 27Ax29B hybrid was better under 110,000 plants/ha, taking the same traits into consideration. The hybrids did not overcome the husked and dehusked ears yield of inbred 27A in both plant densities.","PeriodicalId":49085,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"13 1","pages":"177-184"},"PeriodicalIF":1.5,"publicationDate":"2003-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88075239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-09-30DOI: 10.12702/1984-7033.V03N03A03
A. Corte, V. Moda‐Cirino, M. Scholz, D. Destro
The objective of the present study was to assess the technological and nutritional quality of seven early common bean cultivars and nine lines. An experiment in completely randomized blocks was carried out in three locations in the wet 2001/2002 growing season. One hundred gram samples were collected from each material at physical maturity to estimate the grain technological and nutritional characteristics and also the weight of 1000 seeds, shape and degree of seed flattening were assessed by the J and H coefficient, respectively. The joint analysis of variance showed significant genotype and environment effect at 1% probability. The genotype x environment interaction was significant at 1% probability for all the characteristics assessed except for the weight of 1000 seeds and the J coefficient. Cooking time showed positive phenotypic and genotypic correlation with percentage of whole grains after cooking, weight of 1000 seeds and seed shape, indicating the possibility of simultaneous selection among these characteristics. However, the total solids in the sauce correlated negatively with these traits that are desirable in the industrial processes of the common bean.
{"title":"Environment effect on grain quality in early common bean cultivars and lines","authors":"A. Corte, V. Moda‐Cirino, M. Scholz, D. Destro","doi":"10.12702/1984-7033.V03N03A03","DOIUrl":"https://doi.org/10.12702/1984-7033.V03N03A03","url":null,"abstract":"The objective of the present study was to assess the technological and nutritional quality of seven early common bean cultivars and nine lines. An experiment in completely randomized blocks was carried out in three locations in the wet 2001/2002 growing season. One hundred gram samples were collected from each material at physical maturity to estimate the grain technological and nutritional characteristics and also the weight of 1000 seeds, shape and degree of seed flattening were assessed by the J and H coefficient, respectively. The joint analysis of variance showed significant genotype and environment effect at 1% probability. The genotype x environment interaction was significant at 1% probability for all the characteristics assessed except for the weight of 1000 seeds and the J coefficient. Cooking time showed positive phenotypic and genotypic correlation with percentage of whole grains after cooking, weight of 1000 seeds and seed shape, indicating the possibility of simultaneous selection among these characteristics. However, the total solids in the sauce correlated negatively with these traits that are desirable in the industrial processes of the common bean.","PeriodicalId":49085,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"15 1","pages":"193-202"},"PeriodicalIF":1.5,"publicationDate":"2003-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86436444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-09-30DOI: 10.12702/1984-7033.V03N03A09
C. Guilherme, Portela de Carvalho, Caio Márcio, Vasconcellos Cordeiro de Almeida, D. C. Cosme, Paulo Fernades Rodrigues Machado
Hybrid cocoa tree (Theobroma cacao L.) adaptability and yield temporal stability were studied to screen agronomically superior varieties for yield, bean quality and resistance to witches’ broom under the ecological conditions of Ouro Preto do Oeste County, Rondonia State, Brazil. Assessments were based on yield component measurements evaluated at maturity in a randomized complete block experiment. Additionally, the possibility of early hybrid selection, at the initial fructification phase, was investigated. For this, the results obtained from both periods was compared. At maturity, the SCA 6 x ICS 1, PA 150 x SIC 328 and IMC 67 x BE 8 hybrids were superior in the simultaneous analysis of total number of healthy fruits (TNHF), total weight of fresh beans (TWFB) and mean weight of fresh beans per fruit (MWFBF). Using Eberhart and Russell’s methodology, PA 150 x SIC 328 had general adaptability and highly predictable performance for TNHF. The SCA 6 x ICS 1 hybrid also had general adaptability, in spite of indicated low predictability. The IMC 67 x BE 8 cross was adapted to unfavorable environments and had high predictability for TNHF. The SCA 6 x ICS 1 and PA 150 x SIC 328 hybrids behaved similarly for TWFB, showing adaptability to favorable environments and high predictability. IMC 67 x BE 8 showed general adaptability and high predictability for this trait. The three hybrids showed general adaptability and high predictability for MWFBF. On the base of mean performance (or i P values of Lin and Binn’ methodology), hybrid selection was also successful using measurements taken at the initial fructification phase. On the other hand, the Eberhart and Russel’s adaptability and stability parameters depended on the period evaluated.
在巴西朗多尼亚州欧鲁普雷图奥多埃斯特县的生态条件下，研究了杂交可可树(Theobroma可可豆L.)的适应性和产量的时间稳定性，以筛选产量、豆子品质和抗黑帚病的农艺优势品种。评估是基于在随机完全块试验中评估成熟时的产量成分测量。此外，还研究了早期杂种选择的可能性，即在果实初始阶段。为此，比较了两个时期的结果。成熟期同时分析健康果总数(TNHF)、鲜豆总重(TWFB)和单果平均鲜豆重(MWFBF)时，SCA 6 × ICS 1、PA 150 × SIC 328和IMC 67 × BE 8均表现优异。使用Eberhart和Russell的方法，pa150 x SIC 328对TNHF具有一般适应性和高度可预测的性能。SCA 6 x ICS 1混合型也具有一般的适应性，尽管显示出低可预测性。imc67 × be8杂交适应不利环境，对TNHF具有较高的可预测性。SCA 6 × ICS 1和PA 150 × SIC 328杂交品种的TWFB表现相似，表现出对有利环境的适应性和高可预测性。imc67 × be8对这一性状表现出一般的适应性和高度的可预测性。3个杂种对MWFBF表现出一般的适应性和较高的可预测性。在平均表现的基础上(或Lin和Binn方法的i P值)，杂种选择也是成功的，利用在结果初期进行的测量。另一方面，Eberhart和Russel的适应性和稳定性参数取决于所评估的时间段。
{"title":"Hybrid cocoa tree adaptability and yield temporal stability in Rondônia State, Brazil","authors":"C. Guilherme, Portela de Carvalho, Caio Márcio, Vasconcellos Cordeiro de Almeida, D. C. Cosme, Paulo Fernades Rodrigues Machado","doi":"10.12702/1984-7033.V03N03A09","DOIUrl":"https://doi.org/10.12702/1984-7033.V03N03A09","url":null,"abstract":"Hybrid cocoa tree (Theobroma cacao L.) adaptability and yield temporal stability were studied to screen agronomically superior varieties for yield, bean quality and resistance to witches’ broom under the ecological conditions of Ouro Preto do Oeste County, Rondonia State, Brazil. Assessments were based on yield component measurements evaluated at maturity in a randomized complete block experiment. Additionally, the possibility of early hybrid selection, at the initial fructification phase, was investigated. For this, the results obtained from both periods was compared. At maturity, the SCA 6 x ICS 1, PA 150 x SIC 328 and IMC 67 x BE 8 hybrids were superior in the simultaneous analysis of total number of healthy fruits (TNHF), total weight of fresh beans (TWFB) and mean weight of fresh beans per fruit (MWFBF). Using Eberhart and Russell’s methodology, PA 150 x SIC 328 had general adaptability and highly predictable performance for TNHF. The SCA 6 x ICS 1 hybrid also had general adaptability, in spite of indicated low predictability. The IMC 67 x BE 8 cross was adapted to unfavorable environments and had high predictability for TNHF. The SCA 6 x ICS 1 and PA 150 x SIC 328 hybrids behaved similarly for TWFB, showing adaptability to favorable environments and high predictability. IMC 67 x BE 8 showed general adaptability and high predictability for this trait. The three hybrids showed general adaptability and high predictability for MWFBF. On the base of mean performance (or i P values of Lin and Binn’ methodology), hybrid selection was also successful using measurements taken at the initial fructification phase. On the other hand, the Eberhart and Russel’s adaptability and stability parameters depended on the period evaluated.","PeriodicalId":49085,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"75 1","pages":"237-244"},"PeriodicalIF":1.5,"publicationDate":"2003-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85980712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-09-30DOI: 10.12702/1984-7033.V03N03A02
J. P. Oliveira, Weber Neves Moreira, J. Duarte, L. Chaves, J. B. Pinheiro
The genotype-environment interaction is generally recognized when the same genotype is assessed in different environments, having a decisive influence in cultivar recommendation. Maize hybrids that are only adapted to particular environments can become a limiting factor to seed production in large scale. One of the main objectives of breeders is the obtainment of a hybrid with high mean yield and good adaptation to different environments. The present study assessed the grain yield stability in thirty-six maize genotypes in ten environments located in Central Brazil in the 1999/2000 growing season. The analysis of variance (ANOVA) detected significance (P<0.01) for the genotype-environment interaction (GE). The AMMI model (additive main effects and multiplicative interaction) was used to assess the additive and multiplicative effects of the interaction. The results indicated homogeneity of environments assessed and high correlation of the hybrid genetic constitution with the yield stability of the three types studied -single, triple and double crosses maize hybrids. The first multiplicative component of the interaction explained more than half (50.6%) of the original sum of squares (SS GE ). Most of the environments were neutral in their contribution to the interaction. The Uberlândia environment contributed positively, while the environment in Patos de Minas contributed negatively to the GE interaction. Most of the genotypes also presented a low contribution to interaction. Some single crosses hybrids presented greater mean yield (10182.0 kg.ha -1 ) while the double crosses hybrids presented greater stability to the
基因型-环境互作是在不同环境下对同一基因型进行评价时普遍认识到的，对品种推荐具有决定性影响。只适应特定环境的玉米杂交种可能成为大规模种子生产的限制因素。育种者的主要目标之一是获得高平均产量和对不同环境有良好适应性的杂交种。本研究评估了1999/2000年巴西中部10种环境下36种玉米基因型的粮食产量稳定性。方差分析(ANOVA)发现基因型-环境相互作用(GE)具有显著性(P<0.01)。采用AMMI模型(可加性主效应和乘性相互作用)评价相互作用的可加性和乘性效应。结果表明，所评价的环境均一性和杂交遗传构成与单交、三交和双交玉米杂交种的产量稳定性高度相关。相互作用的第一个乘法分量解释了原始平方和(SS GE)的一半以上(50.6%)。大多数环境对互动的贡献是中性的。uberlindia的环境对GE的相互作用有积极作用，而Patos de Minas的环境对GE的相互作用有消极作用。大多数基因型对互作的贡献也很低。一些单交杂交种的平均产量更高(10182.0 kg)。Ha -1)，而双交杂交种表现出更大的稳定性
{"title":"Genotype-environment interaction in maize hybrids: an application of the AMMI model","authors":"J. P. Oliveira, Weber Neves Moreira, J. Duarte, L. Chaves, J. B. Pinheiro","doi":"10.12702/1984-7033.V03N03A02","DOIUrl":"https://doi.org/10.12702/1984-7033.V03N03A02","url":null,"abstract":"The genotype-environment interaction is generally recognized when the same genotype is assessed in different environments, having a decisive influence in cultivar recommendation. Maize hybrids that are only adapted to particular environments can become a limiting factor to seed production in large scale. One of the main objectives of breeders is the obtainment of a hybrid with high mean yield and good adaptation to different environments. The present study assessed the grain yield stability in thirty-six maize genotypes in ten environments located in Central Brazil in the 1999/2000 growing season. The analysis of variance (ANOVA) detected significance (P<0.01) for the genotype-environment interaction (GE). The AMMI model (additive main effects and multiplicative interaction) was used to assess the additive and multiplicative effects of the interaction. The results indicated homogeneity of environments assessed and high correlation of the hybrid genetic constitution with the yield stability of the three types studied -single, triple and double crosses maize hybrids. The first multiplicative component of the interaction explained more than half (50.6%) of the original sum of squares (SS GE ). Most of the environments were neutral in their contribution to the interaction. The Uberlândia environment contributed positively, while the environment in Patos de Minas contributed negatively to the GE interaction. Most of the genotypes also presented a low contribution to interaction. Some single crosses hybrids presented greater mean yield (10182.0 kg.ha -1 ) while the double crosses hybrids presented greater stability to the","PeriodicalId":49085,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"142 1","pages":"185-192"},"PeriodicalIF":1.5,"publicationDate":"2003-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77849846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-09-30DOI: 10.12702/1984-7033.V03N03A07
A. M. Correa, M. C. Gonçalves, D. Destro, L. C. Ferreira, T. A. Sobrinho
Sixteen common bean genotypes, commercial varieties and advanced lines, were assessed at the Nucleus of Agrarian Sciences at the Federal University of Mato Grosso do Sul, Dourados campus, in the 'dryharvest 2000/2001. Genetic and phenotypic parameters in control of morpho-physiological traits and yield components were estimated. These parameters were estimated at plant level. Variance estimates between and within genotypes and at plot level were obtained in randomized complete block experiments. Three replicates of each genotype were available for the calculations. The populations under study had wide genetic variability for all the traits studied whereas the main stem length was the most promising trait for selection and days to maturity was the least promising. The plot level analysis showed greater precision than plant level analysis.
{"title":"Estimates of genetic parameters in common bean genotypes","authors":"A. M. Correa, M. C. Gonçalves, D. Destro, L. C. Ferreira, T. A. Sobrinho","doi":"10.12702/1984-7033.V03N03A07","DOIUrl":"https://doi.org/10.12702/1984-7033.V03N03A07","url":null,"abstract":"Sixteen common bean genotypes, commercial varieties and advanced lines, were assessed at the Nucleus of Agrarian Sciences at the Federal University of Mato Grosso do Sul, Dourados campus, in the 'dryharvest 2000/2001. Genetic and phenotypic parameters in control of morpho-physiological traits and yield components were estimated. These parameters were estimated at plant level. Variance estimates between and within genotypes and at plot level were obtained in randomized complete block experiments. Three replicates of each genotype were available for the calculations. The populations under study had wide genetic variability for all the traits studied whereas the main stem length was the most promising trait for selection and days to maturity was the least promising. The plot level analysis showed greater precision than plant level analysis.","PeriodicalId":49085,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"1 1","pages":"223-230"},"PeriodicalIF":1.5,"publicationDate":"2003-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89410628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-06-30DOI: 10.12702/1984-7033.V03N02A07
M. P. Guerra, M. Rogalski, Aparecido Lima da Silva
Embryo culture and recovery are techniques used in basic and applied studies of morphogenesis, in vitro propagation and genetic breeding in the Prunus genus. The present study assessed the germination and organogenetic development of embryos in culture media with different concentrations of GA 3 as well as the in vitro multiplication rate of clones of the ‘Capdeboscq’ rootstock. Embryonic axes were excised and inoculated in Lepoivre culture medium supplemented with BAP (0.1 mg L -1 ), NAA (0.1 mg L -1 ) and GA 3 (0.0; 1.0; 2.5; 5.0; 7.5 and 10.0 mg L -1 ). The in vitro multiplication potential of four clones was assessed after embryo germination using the double phase multiplication method in the last subculture. The results showed a mean of 70.7% in vitro germination. GA 3 was not effective in the germination, but induced greater elongation of the embryo and inhibited the root development. The clones obtained in the embryo culture showed high potential for in vitro multiplication with rates of 6.9 and 14.9 shoots per explant.
胚胎培养和恢复是李属植物形态发生、离体繁殖和遗传育种的基础和应用研究技术。研究了不同浓度ga3培养基对‘Capdeboscq’砧木胚萌发和器官发育的影响，并对其无性系的体外增殖率进行了研究。取胚轴，接种于添加BAP (0.1 mg L -1)、NAA (0.1 mg L -1)和ga3 (0.0;1.0;2.5;5.0;7.5和10.0 mg L -1)。在最后一次传代培养中，采用双相增殖法测定了4个无性系胚萌发后的体外增殖潜力。结果表明，离体萌发率平均为70.7%。GA 3对种子萌发无明显影响，但能促进胚伸长，抑制根发育。在胚培养中获得的无性系具有较高的离体增殖潜力，每个外植体分别有6.9和14.9个芽。
{"title":"Embryo culture and in vitro clonal multiplication of Prunus 'Capdeboscq' rootstock","authors":"M. P. Guerra, M. Rogalski, Aparecido Lima da Silva","doi":"10.12702/1984-7033.V03N02A07","DOIUrl":"https://doi.org/10.12702/1984-7033.V03N02A07","url":null,"abstract":"Embryo culture and recovery are techniques used in basic and applied studies of morphogenesis, in vitro propagation and genetic breeding in the Prunus genus. The present study assessed the germination and organogenetic development of embryos in culture media with different concentrations of GA 3 as well as the in vitro multiplication rate of clones of the ‘Capdeboscq’ rootstock. Embryonic axes were excised and inoculated in Lepoivre culture medium supplemented with BAP (0.1 mg L -1 ), NAA (0.1 mg L -1 ) and GA 3 (0.0; 1.0; 2.5; 5.0; 7.5 and 10.0 mg L -1 ). The in vitro multiplication potential of four clones was assessed after embryo germination using the double phase multiplication method in the last subculture. The results showed a mean of 70.7% in vitro germination. GA 3 was not effective in the germination, but induced greater elongation of the embryo and inhibited the root development. The clones obtained in the embryo culture showed high potential for in vitro multiplication with rates of 6.9 and 14.9 shoots per explant.","PeriodicalId":49085,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"86 1","pages":"141-148"},"PeriodicalIF":1.5,"publicationDate":"2003-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76340332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-06-30DOI: 10.12702/1984-7033.V03N02A02
A. Aguiar, F. Bortolozo, Mário Luiz, T. Moraes, J. Costa
Two progeny tests of two goncalo-alves (Astronium fraxinifolium) populations were evaluated in March 1996 at Selviria, Mato Grosso do Sul State, Brazil. A randomized complete block design with 30 treatments (progenies) and five replications was used. Plots were formed by 10 plants arranged in a alternate single rows. Spacing was 3.0m between plants within rows and between rows. Data on 10 silvicultural traits were obtained to help in “ex situ” efforts of genetic conservation of the species. The progenies had excellent performance in the used growing system. Both populations presented genetic variability for all analyzed traits, although no significant difference between the populations was detected. It is therefore recommended that both populations be considered as a single population for “ex situ” genetic conservation purposes. In a genetic breeding program involving those populations, plant height is the trait with greater chances of obtaining the selection gain in level of average of families.
{"title":"Genetic variation in Astronium fraxinifolium populations in consortium","authors":"A. Aguiar, F. Bortolozo, Mário Luiz, T. Moraes, J. Costa","doi":"10.12702/1984-7033.V03N02A02","DOIUrl":"https://doi.org/10.12702/1984-7033.V03N02A02","url":null,"abstract":"Two progeny tests of two goncalo-alves (Astronium fraxinifolium) populations were evaluated in March 1996 at Selviria, Mato Grosso do Sul State, Brazil. A randomized complete block design with 30 treatments (progenies) and five replications was used. Plots were formed by 10 plants arranged in a alternate single rows. Spacing was 3.0m between plants within rows and between rows. Data on 10 silvicultural traits were obtained to help in “ex situ” efforts of genetic conservation of the species. The progenies had excellent performance in the used growing system. Both populations presented genetic variability for all analyzed traits, although no significant difference between the populations was detected. It is therefore recommended that both populations be considered as a single population for “ex situ” genetic conservation purposes. In a genetic breeding program involving those populations, plant height is the trait with greater chances of obtaining the selection gain in level of average of families.","PeriodicalId":49085,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"2011 1","pages":"95-106"},"PeriodicalIF":1.5,"publicationDate":"2003-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86325497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-06-30DOI: 10.12702/1984-7033.V03N02A09
F. Moreira, Marcelo Borghezan, Aparecido Lima da Silva
A simple and non-destructive carbon dissimilation method to assess plant cell growth was tested for its in vitro suitability for grape vines. In vitro culture plants can either use atmospheric CO 2 or the sugars present in the culture medium as carbon source. Carbon dissimilation predicts that the sugar metabolism of the culture medium results in a net weight loss of the culture flask contents and may thus be used to follow culture growth. The ‘Paulsen 1103’ grapevine rootstock was introduced and multiplied in vitro by the auxiliary shoot methodology. Tubes with and without plants were weighed daily throughout an experimental growth period of 60 days. Results from the in vitro plants showed that the variety studied presented dissimilation and assimilation activity over the culture period and therefore presented in vitro fotomixotrophy. There were three distinct growth phases: an initial phase, during the first 10 days of culture, where there was intense weight loss by dissimilation; a second stationary phase, which lasted approximately 10 days, and a final phase of 40 days, where an important weight gain was observed (37.68 mg). Thus carbon use and micropropagated plant growth could be assessed quickly and efficiently.
{"title":"Assessment of the carbon dissimilation methodology in the in vitro growth of the 'Paulsen 1103' grapevine","authors":"F. Moreira, Marcelo Borghezan, Aparecido Lima da Silva","doi":"10.12702/1984-7033.V03N02A09","DOIUrl":"https://doi.org/10.12702/1984-7033.V03N02A09","url":null,"abstract":"A simple and non-destructive carbon dissimilation method to assess plant cell growth was tested for its in vitro suitability for grape vines. In vitro culture plants can either use atmospheric CO 2 or the sugars present in the culture medium as carbon source. Carbon dissimilation predicts that the sugar metabolism of the culture medium results in a net weight loss of the culture flask contents and may thus be used to follow culture growth. The ‘Paulsen 1103’ grapevine rootstock was introduced and multiplied in vitro by the auxiliary shoot methodology. Tubes with and without plants were weighed daily throughout an experimental growth period of 60 days. Results from the in vitro plants showed that the variety studied presented dissimilation and assimilation activity over the culture period and therefore presented in vitro fotomixotrophy. There were three distinct growth phases: an initial phase, during the first 10 days of culture, where there was intense weight loss by dissimilation; a second stationary phase, which lasted approximately 10 days, and a final phase of 40 days, where an important weight gain was observed (37.68 mg). Thus carbon use and micropropagated plant growth could be assessed quickly and efficiently.","PeriodicalId":49085,"journal":{"name":"Crop Breeding and Applied Biotechnology","volume":"14 1","pages":"157-162"},"PeriodicalIF":1.5,"publicationDate":"2003-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82866778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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