Particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) are critical to soil organic carbon (SOC) accumulation and stabilization, yet their responses to intercropping duration and underlying microbial drivers remain poorly understood. Using a long-term apple orchard experiment on the Loess Plateau, this study evaluated white clover intercropping of 8 years (8-yr) and 16 years (16-yr) on POC and MAOC contents across the 0–60 cm soil profile, and examined accumulation mechanisms through microbial life-history strategies, enzyme activities, and microbial carbon pump efficiency. The results demonstrate that MAOC (66–90 %) accounted for a larger proportion of SOC than POC (10–34 %). In the 0–20 cm soil layer, MAOC primarily drove SOC increases induced by intercropping. In deeper soil layers, 8-yr increased POC in the 20–40 and 40–60 cm but did not alter MAOC, whereas 16 yr increased MAOC by 62 % and 34 %, respectively. Intercropping increased microbial necromass carbon (MNC), but the extent varied with intercropping duration and soil depth. Specifically, 8-yr increased both bacterial and fungal necromass (BNC and FNC) in the 0–40 cm soil layer. In contrast, 16-yr enhanced MNC across the entire 0–60 cm profile and notably promoted the accumulation of BNC in 40–60 cm soil layers. Random forest and path modeling indicated stronger links of microbial necromass and resource-acquisitive traits to MAOC than POC. These findings reveal duration-dependent shifts in SOC fraction dynamics regulated by microbial life-history strategies and MCP efficiency. Our work provides a mechanistic basis for optimizing intercropping duration to enhance SOC sequestration in intercropping systems.
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