Incorporating straw or straw-derived biochar is recognized as a promising strategy to enhance soil quality and promote sustainable agricultural development. However, the sustained effects of straw and biochar amendments on crop productivity, greenhouse gas (GHG) emissions and soil multifunctionality remain controversial, largely due to the lack of comparative studies between raw straw and biochar produced from an equivalent amount of straw. To address this issue, a five-year field experiment was conducted with three treatments—CK (no incorporation), SI (continuous raw straw incorporation), and BI (continuous incorporation of biochar derived from an equivalent amount of straw)—to evaluate whether converting raw straw into biochar for soil application provides greater benefits in mitigating GHG emissions and improving soil quality, crop yield, and water productivity. The results showed that both SI and BI significantly improved soil physical (bulk density, porosity, field capacity), chemical (pH, organic carbon, nutrient levels), and biological (microbial biomass carbon and nitrogen) properties, leading to increases in soil ecosystem multifunctionality by 222.8 % and 251.8 %, respectively, compared with CK. Additionally, SI consistently elevated N2O emissions, whereas BI generally reduced N2O emissions relative to CK. Both SI and BI increased CO2 emissions and significantly enhanced crop yield, with SI and BI increasing grain yield by 8.3 % and 25.6 %, and water productivity by 12.2 % and 24.8 %, respectively, in the maize-wheat rotation system compared to CK. As a consequence, SI and BI increased the global warming potential (GWP) by 22.4 % and 6.1 %, respectively, while SI increased greenhouse gas intensity (GHGI) by 12.6 % and BI reduced it by 16.1 %, relative to CK. Notably, continuous incorporation of straw and biochar resulted in a cumulative effect (residual effect + current-season effect) on N2O and CO2 emissions. The residual effect on N2O and CO2 emissions persisted for 3 and 4 years, respectively, under SI, and extended up to 7 years for both gases under BI. Overall, these findings demonstrate that converting straw into biochar for soil incorporation not only enhances soil quality and sustains high crop productivity but also contributes to mitigating GHG emissions. This study highlights the importance of considering long-term dynamics in straw and biochar management and underscores biochar's potential as a sustainable strategy for climate change mitigation.
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