CORRIGENDUM by Snezana Mladenovic Drinic, Editor of the journal Genetika due to technical mistake reference are not correct for paper titled CYTOGENETIC ASPECTS OF MISCARRIAGE authors Petrovic B. and M. Komnenic Radovanovic published in the journal Genetika, (2021). - Genetika, Vol 53, No.2, 663-670. Original scientific paper https://doi.org/10.2298/GENSR2102663P and correct reference are listed below.
{"title":"Corrigendum: Cytogenetic aspects of miscarriage, Authors Petrovic B. and M. Komnenic Radovanovic, Published in the journal Genetika, (2021). - Genetika, vol 53, no.2, 663-670","authors":"E. Editorial","doi":"10.2298/gensr2202977e","DOIUrl":"https://doi.org/10.2298/gensr2202977e","url":null,"abstract":"CORRIGENDUM by Snezana Mladenovic Drinic, Editor of the journal Genetika due to technical mistake reference are not correct for paper titled CYTOGENETIC ASPECTS OF MISCARRIAGE authors Petrovic B. and M. Komnenic Radovanovic published in the journal Genetika, (2021). - Genetika, Vol 53, No.2, 663-670. Original scientific paper https://doi.org/10.2298/GENSR2102663P and correct reference are listed below. <br><br><font color=\"red\"><b> Link to the corrected article <u><a href=\"http://dx.doi.org/10.2298/GENSR2102663P\">10.2298/GENSR2102663P</a></b></u>","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"57 36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68294867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Petrović, Sercan Ergün, Marija Djordjic-Crnogorac, T. Stanojković, E. Mališić, I. Matić
Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.
{"title":"Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells","authors":"N. Petrović, Sercan Ergün, Marija Djordjic-Crnogorac, T. Stanojković, E. Mališić, I. Matić","doi":"10.2298/gensr2203249p","DOIUrl":"https://doi.org/10.2298/gensr2203249p","url":null,"abstract":"Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68295984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Adeleke, K. Bello, Ilesanmi Akinyemi, O. Irekhore, B. Ilori, O. Adeyeye, S. Famakinde
The study of Fulani ecotype (indigenous) chicken becomes necessary for proper understanding of its potentials for future breeding programmes in Nigeria. Sixty adult Fulani ecotype chickens comprising 22 males and 38 females were sourced from the Fulani kraal at Opeji in Odeda Local Government area of Ogun State and used to determine their qualitative and quantitative traits. Twenty (20) cocks out of the original 22 were selected randomly from the population for semen quality assessment. Results indicated that polydactyly was observed on 26.67% of the population. Brown and dark brown plumage colours were dominant and had an occurrence of 48.34% and 33.33%, respectively. Comb type was predominantly single (85%) while 46.67% of the population had Spur. Plumage colour influenced (p<0.05) comb length, comb width, earlobe width and tail length while shank colour had significant (p<0.05) effect on earlobe length, wattle length, wattle width, tail length. Plumage colour had significant (p<0.05-0.01) effect on sperm motility, semen pH, sperm concentration and semen volume but not (p>0.05) on livability and abnormality. Brown plumage had the highest (p<0.05) sperm motility (88.50?0.89%) and pH (7.01?0.01) which was similar to brown black. Fulani cock with light brown plumage colour had the highest (p<0.05) sperm concentration (1.90?0.11?109/ml) while dark brown had the least value 1.54?0.11? 109/ml. Light brown chicken recorded higher (p<0.05) semen volume (0.21?0.01/ml) when compared with the dark brown chicken (0.14?0.01/ml). Medium to high positive correlations were observed among all linear body measurements. The study concluded that there existed high phenotypic diversity in Fulani chickens and could be exploited for improved breeding and conservation programmes in Nigeria. The body weight and other linear body measurements of the chickens are highly and positively correlated. However, lighter plumage Fulani ecotype cocks could be adopted in multiplication program where semen motility and concentration are paramount.
{"title":"Morphological variations in qualitative and quantitative traits of Fulani ecotype chickens in Nigeria","authors":"M. Adeleke, K. Bello, Ilesanmi Akinyemi, O. Irekhore, B. Ilori, O. Adeyeye, S. Famakinde","doi":"10.2298/gensr2201043a","DOIUrl":"https://doi.org/10.2298/gensr2201043a","url":null,"abstract":"The study of Fulani ecotype (indigenous) chicken becomes necessary for proper understanding of its potentials for future breeding programmes in Nigeria. Sixty adult Fulani ecotype chickens comprising 22 males and 38 females were sourced from the Fulani kraal at Opeji in Odeda Local Government area of Ogun State and used to determine their qualitative and quantitative traits. Twenty (20) cocks out of the original 22 were selected randomly from the population for semen quality assessment. Results indicated that polydactyly was observed on 26.67% of the population. Brown and dark brown plumage colours were dominant and had an occurrence of 48.34% and 33.33%, respectively. Comb type was predominantly single (85%) while 46.67% of the population had Spur. Plumage colour influenced (p<0.05) comb length, comb width, earlobe width and tail length while shank colour had significant (p<0.05) effect on earlobe length, wattle length, wattle width, tail length. Plumage colour had significant (p<0.05-0.01) effect on sperm motility, semen pH, sperm concentration and semen volume but not (p>0.05) on livability and abnormality. Brown plumage had the highest (p<0.05) sperm motility (88.50?0.89%) and pH (7.01?0.01) which was similar to brown black. Fulani cock with light brown plumage colour had the highest (p<0.05) sperm concentration (1.90?0.11?109/ml) while dark brown had the least value 1.54?0.11? 109/ml. Light brown chicken recorded higher (p<0.05) semen volume (0.21?0.01/ml) when compared with the dark brown chicken (0.14?0.01/ml). Medium to high positive correlations were observed among all linear body measurements. The study concluded that there existed high phenotypic diversity in Fulani chickens and could be exploited for improved breeding and conservation programmes in Nigeria. The body weight and other linear body measurements of the chickens are highly and positively correlated. However, lighter plumage Fulani ecotype cocks could be adopted in multiplication program where semen motility and concentration are paramount.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68291625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Building natural classifications for genera with complex morphological variations such as Centaurea needs the support of molecular analysis and knowledge of morphological character diversification. Here we use parsimony and Bayesian analyses of nuclear ribosomal ITS sequences to gain insight into the sectional relationships and character evolution in Centaurea sections Chartolepis, Cheirolepis, Cynaroides, Grosheimia, Microlophus, Paraphysis, Phaeopappus, Plumosipappus, Pseudoserida, Pseudophaeopappus, Ptheracantha, Ptosimopappus, and Rhizocalathium, from Turkey. In particular, we increase sampling in Turkish endemic species of Centaurea sect. Cynaroides and related taxa. The new ITS sequences for 26 taxa from 48 locations are given. Sequences of 17 taxa are given here the first time. The phylogeny derived from ITS data provides a reasonably resolved and reasonably supported phylogenetic framework for the genus. Our analysis shows characters traditionally used in Centaurea taxonomy such as flower colour, appendage width, spine length, and pappus length evolved several times independently and show a lot of homoplasies. However, we can confirm the validity of character combinations for a reliable subgeneric classification of Centaurea.
{"title":"Morphological homoplasy and sectional polyphyly in Centaurea sect. Cynaroides and related taxa","authors":"M. Bona, M. Hughes","doi":"10.2298/gensr2202515b","DOIUrl":"https://doi.org/10.2298/gensr2202515b","url":null,"abstract":"Building natural classifications for genera with complex morphological variations such as Centaurea needs the support of molecular analysis and knowledge of morphological character diversification. Here we use parsimony and Bayesian analyses of nuclear ribosomal ITS sequences to gain insight into the sectional relationships and character evolution in Centaurea sections Chartolepis, Cheirolepis, Cynaroides, Grosheimia, Microlophus, Paraphysis, Phaeopappus, Plumosipappus, Pseudoserida, Pseudophaeopappus, Ptheracantha, Ptosimopappus, and Rhizocalathium, from Turkey. In particular, we increase sampling in Turkish endemic species of Centaurea sect. Cynaroides and related taxa. The new ITS sequences for 26 taxa from 48 locations are given. Sequences of 17 taxa are given here the first time. The phylogeny derived from ITS data provides a reasonably resolved and reasonably supported phylogenetic framework for the genus. Our analysis shows characters traditionally used in Centaurea taxonomy such as flower colour, appendage width, spine length, and pappus length evolved several times independently and show a lot of homoplasies. However, we can confirm the validity of character combinations for a reliable subgeneric classification of Centaurea.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68293494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Afsaneh Jalilzadeh, S. Hamdi, Y. Asri, M. Assadi, A. Iranbakhsh
Chenopodiaceae is a large, diverse and cosmopolitan family within the order Carophyllales. The majority of Chenopodiaceae species are adapted to saline, hypersaline, xerophytic and xerohalophytic communities or ruderal habitats. The family Chenopodiaceae is stenopolinous the pollen grains are monad and exclusively polypantoporate with tiny spinules on both tectum and operculum. Pollen morphology of six genera of Chenopodiaceae (Seidlitzia, Atriplex, Bassia, Salsola, Krascheninnikovia and Spinacia) have been studied in details. These plant species were collected from different phytogeographical regions of Iran. The palynological investigation was done using scanning electron microscopy (SEM) techniques. Pollen characters studied in this study include pollen and pore diameters, number and density of apertures, interporal distance (chord), chord/pollen diameter ratio, pore diameter/pollen diameter ratio as well as spinule density on tectum and operculum. We used different multivariate statistical methods to reveal the species relationships. Ward clustering analyses have been done to check out the relationship among the species. The shapes of pollen grains were radially symmetrical, isopolar, pantopolyporate and spheroidal. Their exine structure is similar. In the Chenopodiaceae, three pollen types have been defined, mainly on the basis of pollen grain size, pore number, number of microechinae on pore membrane, the density of spinules, and pore edge shape.
{"title":"Palynological analysis some species of Chenopodiaceae and its systematic implications using scanning electron microscopy","authors":"Afsaneh Jalilzadeh, S. Hamdi, Y. Asri, M. Assadi, A. Iranbakhsh","doi":"10.2298/gensr2202539j","DOIUrl":"https://doi.org/10.2298/gensr2202539j","url":null,"abstract":"Chenopodiaceae is a large, diverse and cosmopolitan family within the order Carophyllales. The majority of Chenopodiaceae species are adapted to saline, hypersaline, xerophytic and xerohalophytic communities or ruderal habitats. The family Chenopodiaceae is stenopolinous the pollen grains are monad and exclusively polypantoporate with tiny spinules on both tectum and operculum. Pollen morphology of six genera of Chenopodiaceae (Seidlitzia, Atriplex, Bassia, Salsola, Krascheninnikovia and Spinacia) have been studied in details. These plant species were collected from different phytogeographical regions of Iran. The palynological investigation was done using scanning electron microscopy (SEM) techniques. Pollen characters studied in this study include pollen and pore diameters, number and density of apertures, interporal distance (chord), chord/pollen diameter ratio, pore diameter/pollen diameter ratio as well as spinule density on tectum and operculum. We used different multivariate statistical methods to reveal the species relationships. Ward clustering analyses have been done to check out the relationship among the species. The shapes of pollen grains were radially symmetrical, isopolar, pantopolyporate and spheroidal. Their exine structure is similar. In the Chenopodiaceae, three pollen types have been defined, mainly on the basis of pollen grain size, pore number, number of microechinae on pore membrane, the density of spinules, and pore edge shape.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68293560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katarina Jovanovic, J. Carkic, N. Nikolic, Nada Novakovic, M. Hadzi-Mihailović, E. Čolak, S. Čakić, J. Milašin
The aim of this study was to evaluate the efficiency of chlorhexidine digluconate solution (CHX) as an adjunct to nonsurgical therapy (NPT) of patients with chronic periodontitis (CP), by analyzing the presence and quantity of periopathogenic microorganisms in subgingival biofilm. DNA was extracted from the subgingival biofilm obtained from 40 patients with CP divided into two groups (NPT+CHX and NPT alone as control) at baseline and 2 months after the therapy. The presence of selected periodontal pathogens (A. actinomycetemcomitans, P. gingivalis, E. corrodens, T. denticola, and T. forsythia) was determined by polymerase chain reaction (PCR), while total bacterial load was assessed by quantitative PCR. The incidence of microorganisms decreased following treatment, both with NPT+CHX and NPT alone, but without reaching statistically significant difference in the NPT group. In the NPT+CHX group, a significant reduction of prevalence of two species: T. denticola (P = 0.008) and T. forsythia (P = 0.016), as well as of total microorganism count (P = 0.002) was observed two months after treatment. In conclusion, the present findings support the use of CHX as adjunctive therapy in CP.
{"title":"Detection and quantification of pathogenic microorganisms in patients with chronic periodontitis before and after treatment with chlorhexidine digluconate","authors":"Katarina Jovanovic, J. Carkic, N. Nikolic, Nada Novakovic, M. Hadzi-Mihailović, E. Čolak, S. Čakić, J. Milašin","doi":"10.2298/gensr2201491j","DOIUrl":"https://doi.org/10.2298/gensr2201491j","url":null,"abstract":"The aim of this study was to evaluate the efficiency of chlorhexidine digluconate solution (CHX) as an adjunct to nonsurgical therapy (NPT) of patients with chronic periodontitis (CP), by analyzing the presence and quantity of periopathogenic microorganisms in subgingival biofilm. DNA was extracted from the subgingival biofilm obtained from 40 patients with CP divided into two groups (NPT+CHX and NPT alone as control) at baseline and 2 months after the therapy. The presence of selected periodontal pathogens (A. actinomycetemcomitans, P. gingivalis, E. corrodens, T. denticola, and T. forsythia) was determined by polymerase chain reaction (PCR), while total bacterial load was assessed by quantitative PCR. The incidence of microorganisms decreased following treatment, both with NPT+CHX and NPT alone, but without reaching statistically significant difference in the NPT group. In the NPT+CHX group, a significant reduction of prevalence of two species: T. denticola (P = 0.008) and T. forsythia (P = 0.016), as well as of total microorganism count (P = 0.002) was observed two months after treatment. In conclusion, the present findings support the use of CHX as adjunctive therapy in CP.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68293663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sima Azadmanesh, J. Mozafari, N. Hasanzadeh, C. Moslemkhani
To find the best inoculation method for evaluation of the resistance in potato genotypes against bacterial blackleg caused by Pectobacterium atrosepticum under in vitro conditions, five inoculation methods were compared. In vitro grown explants of five potato genotypes were inoculated with different inoculation methods, then placed on MS solid medium and incubated at 23?C with 70% relative humidity under the light regime of 16 hours a day. After the appearance of symptoms, the efficiency of inoculation methods was then recorded based on the severity of disease symptoms in potato genotypes: Farmosa, Agria, Picaso, Marfona and a wild potato genotype ?Solanum phureja'. Plantlets inoculated by piercing the crown with sterile toothpick inoculated in bacterial suspension of 108 cfu/ml showed the most severe symptoms. Based on all experiments, cultivar Marfona showed higher resistance among all cultivars and, cultivar Agria was the most susceptible. Finally, after witnessing the reactions of different varieties to inoculation methods and comparing them with previous evaluations of resistance in greenhouse conditions, the crown treatment employing sterile toothpick after infection in 108 cfu/ml bacterial suspension was selected and introduced as the best evaluation method of in vitro potato explants against blackleg.
{"title":"Comparing inoculation methods for in vitro evaluation of resistance to blackleg disease in potato cultivars","authors":"Sima Azadmanesh, J. Mozafari, N. Hasanzadeh, C. Moslemkhani","doi":"10.2298/gensr2202705a","DOIUrl":"https://doi.org/10.2298/gensr2202705a","url":null,"abstract":"To find the best inoculation method for evaluation of the resistance in potato genotypes against bacterial blackleg caused by Pectobacterium atrosepticum under in vitro conditions, five inoculation methods were compared. In vitro grown explants of five potato genotypes were inoculated with different inoculation methods, then placed on MS solid medium and incubated at 23?C with 70% relative humidity under the light regime of 16 hours a day. After the appearance of symptoms, the efficiency of inoculation methods was then recorded based on the severity of disease symptoms in potato genotypes: Farmosa, Agria, Picaso, Marfona and a wild potato genotype ?Solanum phureja'. Plantlets inoculated by piercing the crown with sterile toothpick inoculated in bacterial suspension of 108 cfu/ml showed the most severe symptoms. Based on all experiments, cultivar Marfona showed higher resistance among all cultivars and, cultivar Agria was the most susceptible. Finally, after witnessing the reactions of different varieties to inoculation methods and comparing them with previous evaluations of resistance in greenhouse conditions, the crown treatment employing sterile toothpick after infection in 108 cfu/ml bacterial suspension was selected and introduced as the best evaluation method of in vitro potato explants against blackleg.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68293796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Study was carried out for determining the reaction of 83 peppers varieties, breeding lines, and local forms /landraces/ to fungal pathogen Verticillium dahliae Kleb. as they are the part of the Balkan Capsicum core collection which was deeply phenotyped in the recent years. The development of the disease was reported twice and high degree of resistance was reported for 41 accessions. The highest percentage of highly resistant group of genotypes was 72%, followed by resistant group with 23%. The infestation in the groups of medium sensitive, sensitive and highly sensitive was respectively 1%, 3%, and 1%. The highly sensitive (0 - 19%), sensitive (20 - 39%) and medium sensitive (40 - 59%) are consisted by the var. blocky, var. ratundum and var. kapia accessions. The group of resistant (60 - 79%) was dominated by var. ratundum and var. blocky. Materials from var. kapia prevail of highly resistant (80 - 100%). The group of pungent peppers accessions was more resistant to Verticillium infestation. On the base of current and previous results may concluded that four accessions were identified as resistant to TMV and non-infested by Verticillium while two ones possessed low infestation by green aphids, trips and cotton bollworm and non-attacked by Verticillium. Other seven genotypes combined lack of infestation by Verticillium wilt with high levels of three and more agronomic and biochemical traits.
{"title":"Assessment of pepper genetic resources for Verticillium wilt resistance","authors":"K. Vasileva, V. Todorova","doi":"10.2298/gensr2202829v","DOIUrl":"https://doi.org/10.2298/gensr2202829v","url":null,"abstract":"Study was carried out for determining the reaction of 83 peppers varieties, breeding lines, and local forms /landraces/ to fungal pathogen Verticillium dahliae Kleb. as they are the part of the Balkan Capsicum core collection which was deeply phenotyped in the recent years. The development of the disease was reported twice and high degree of resistance was reported for 41 accessions. The highest percentage of highly resistant group of genotypes was 72%, followed by resistant group with 23%. The infestation in the groups of medium sensitive, sensitive and highly sensitive was respectively 1%, 3%, and 1%. The highly sensitive (0 - 19%), sensitive (20 - 39%) and medium sensitive (40 - 59%) are consisted by the var. blocky, var. ratundum and var. kapia accessions. The group of resistant (60 - 79%) was dominated by var. ratundum and var. blocky. Materials from var. kapia prevail of highly resistant (80 - 100%). The group of pungent peppers accessions was more resistant to Verticillium infestation. On the base of current and previous results may concluded that four accessions were identified as resistant to TMV and non-infested by Verticillium while two ones possessed low infestation by green aphids, trips and cotton bollworm and non-attacked by Verticillium. Other seven genotypes combined lack of infestation by Verticillium wilt with high levels of three and more agronomic and biochemical traits.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68294444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Forty-one fine-scented rice genotypes were evaluated for 18 agro-morphological and quality characters for characterization, and 21 quantitative characters were evaluated for principal component analysis in R-studio software. Characterization of agro-morphological traits viz., plant height, days to 50% flowering, panicle length, number of effective tillers per plant, test weight, grain length, grain breadth, grain L: B ratio, kernel length, kernel breadth, kernel dimensions, awns, colour of awns, distribution of awns, and quality traits viz., alkali spreading value, gel consistency, grain aroma, and amylose content showed huge diversity among the genotypes. PCA revealed that PC1 showed the highest amount of variance (32.0%) followed by PC2 (15.7%), PC3 (9.0%), PC4 (8.1%), PC5 (7.8%), PC6 (5.4%) for quantitative characters. Out of 21 principal components, only 6 showed an eigenvalue greater than 1 and contributes about 78.1% total variance Genotypes in PC1 showed higher values for grain L: B ratio and kernel L: B ratio. Similarly, PC2 showed higher variable values for characters like test weight, kernel length, grain length, grain breadth, alkali spreading value, grain yield per plot and amylose content. PC3 for harvest index, panicle length, gel consistency, no. of effective tillers per plant and head rice recovery. PC4 for characters like plant height, kernel breadth and days to 50% flowering. PC5 for characters like kernel elongation ratio, and filled grains per panicle. PC6 for characters like no. of tillers in a square meter and no. of panicles in a square meter. This pre-breeding characterization study may be useful in finding potential genotypes which are having both yield and quality characters which may be useful in breeding for high-yielding varieties with good-quality characters.
{"title":"Agro-morphological and biochemical characterization and principal component analysis for yield and quality characters in fine-scented rice genotypes","authors":"R. Kondi, Sonali Kar, Soumya Surakanti","doi":"10.2298/gensr2203005k","DOIUrl":"https://doi.org/10.2298/gensr2203005k","url":null,"abstract":"Forty-one fine-scented rice genotypes were evaluated for 18 agro-morphological and quality characters for characterization, and 21 quantitative characters were evaluated for principal component analysis in R-studio software. Characterization of agro-morphological traits viz., plant height, days to 50% flowering, panicle length, number of effective tillers per plant, test weight, grain length, grain breadth, grain L: B ratio, kernel length, kernel breadth, kernel dimensions, awns, colour of awns, distribution of awns, and quality traits viz., alkali spreading value, gel consistency, grain aroma, and amylose content showed huge diversity among the genotypes. PCA revealed that PC1 showed the highest amount of variance (32.0%) followed by PC2 (15.7%), PC3 (9.0%), PC4 (8.1%), PC5 (7.8%), PC6 (5.4%) for quantitative characters. Out of 21 principal components, only 6 showed an eigenvalue greater than 1 and contributes about 78.1% total variance Genotypes in PC1 showed higher values for grain L: B ratio and kernel L: B ratio. Similarly, PC2 showed higher variable values for characters like test weight, kernel length, grain length, grain breadth, alkali spreading value, grain yield per plot and amylose content. PC3 for harvest index, panicle length, gel consistency, no. of effective tillers per plant and head rice recovery. PC4 for characters like plant height, kernel breadth and days to 50% flowering. PC5 for characters like kernel elongation ratio, and filled grains per panicle. PC6 for characters like no. of tillers in a square meter and no. of panicles in a square meter. This pre-breeding characterization study may be useful in finding potential genotypes which are having both yield and quality characters which may be useful in breeding for high-yielding varieties with good-quality characters.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68295319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Caro-Petrovic, D. Ružić-Muslić, Nevena Maksimović, B. Ristanović, I. Ćosić, Dusica Ostojic-Andric, D. Nikšić
IGFBP-3 is responsible for the multiple effects of growth factors in most mammalian species and is considered the major transport factor of growth, used as a marker for different body functions such as growth, metabolism, reproduction, body weight control, immunity, energy balance, and so on. Considered as a candidate gene, used as a marker for the growth and production traits as its essential role in the growth and development of the animals. For the DNA extraction, the blood samples are obtained in the jugular vein using a 10 ml vacutainer containing EDTA as a coagulant in the blood collection of each animal Mis breed of sheep (M), Ile de France (F), and Wurttemberg (W). Isolation of DNA performed using the extraction kit (Quick DNA kit) with primers set the Forward and Reverse. The body weights of lambs from birth to 90 days of age, also been calculated. The results of the agarose gel electrophoresis of PCR amplified IGFBP-3 genes for sheep populations Wurttemberg (W), Mis (M), and Ile de France (F) had 654 bp. In our results showed an absence of polymorphism of the IGFBP-3 gene on the tested sheep populations. The results that there is no polymorphism between the examined sheep breeds, in terms of IGFBP-3 genes, we were interested whether there are differences in the body development of lambs of the mentioned populations because IGFBP-3 is related to the growth of animals. All three breeds have similar weights and growth dynamics, which could link to the growth hormone. Analyzing obtained results, we can suggest that absence of a large difference in the growth of the three breeds of sheep W, M, F does not have to be related to the absence of polymorphism of the IGFBP-3 gene but also other genetic and non-genetic factors can affect this trait. To detect the association between genetic polymorphism in IGFBP-3 genes and body development in lambs, DNA sequencing is required, which will be the subject of our future research.
{"title":"PCR-RLFP on IGFBP -3 gene and its association on growth performance of lambs reared intensively","authors":"V. Caro-Petrovic, D. Ružić-Muslić, Nevena Maksimović, B. Ristanović, I. Ćosić, Dusica Ostojic-Andric, D. Nikšić","doi":"10.2298/gensr2203111p","DOIUrl":"https://doi.org/10.2298/gensr2203111p","url":null,"abstract":"IGFBP-3 is responsible for the multiple effects of growth factors in most mammalian species and is considered the major transport factor of growth, used as a marker for different body functions such as growth, metabolism, reproduction, body weight control, immunity, energy balance, and so on. Considered as a candidate gene, used as a marker for the growth and production traits as its essential role in the growth and development of the animals. For the DNA extraction, the blood samples are obtained in the jugular vein using a 10 ml vacutainer containing EDTA as a coagulant in the blood collection of each animal Mis breed of sheep (M), Ile de France (F), and Wurttemberg (W). Isolation of DNA performed using the extraction kit (Quick DNA kit) with primers set the Forward and Reverse. The body weights of lambs from birth to 90 days of age, also been calculated. The results of the agarose gel electrophoresis of PCR amplified IGFBP-3 genes for sheep populations Wurttemberg (W), Mis (M), and Ile de France (F) had 654 bp. In our results showed an absence of polymorphism of the IGFBP-3 gene on the tested sheep populations. The results that there is no polymorphism between the examined sheep breeds, in terms of IGFBP-3 genes, we were interested whether there are differences in the body development of lambs of the mentioned populations because IGFBP-3 is related to the growth of animals. All three breeds have similar weights and growth dynamics, which could link to the growth hormone. Analyzing obtained results, we can suggest that absence of a large difference in the growth of the three breeds of sheep W, M, F does not have to be related to the absence of polymorphism of the IGFBP-3 gene but also other genetic and non-genetic factors can affect this trait. To detect the association between genetic polymorphism in IGFBP-3 genes and body development in lambs, DNA sequencing is required, which will be the subject of our future research.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68295838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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