S. Jovicic-Pavlovic, S. Simić-Ogrizović, Z. Bukumirić, Milena Eric, Natalija Pavlovic, Boba Kotlica, I. Novaković
Fetuin A is a major systemic inhibitor of vascular calcifications. The aim of this study was to examine association of single nucleotide polymorphisms (SNP) in the gene for fetuin-A with fetuin-A serum levels, coronary arteries calcification (CAC) and mortality in renal transplant (RT) and chronic kidney (CKD) patients. This study included 88 patients (42 stable RT patients at least 6 months after transplantation and 46 CKD patients, stage 2-5 not requiring dialysis) followed five years. Detection and analysis of fetuin A gene polymorphisms in positions C742T (Thr248Met; rs4917) and C766G (Thr256Ser; rs4918) were performed using PCR method. Respondents with allele 742T had at the same time 766G. Combined genotypes TT/GG had lower serum fetuin A levels than CT /CG and CC/CC. Predictors of CAC in univariate analysis were age (p=0,000), serum fetuin-A levels (p=0.011) and rs 4917 polymorphism (p=0.021) while multivariate determined age (p=0.001) and fetuin-A levels (p=0.031). Patients who were homozygous for variant 742T and 766G (combined genotype TT/GG) had lowest survival rate. Our results suggest that allele 742T and 766G in gene for fetuin-A were associated with lower serum fetuin-A levels, higher CAC occurrence and higher mortality rate in RT and CKD patients.
{"title":"Impact of the fetuin gene polymorphisms in coronary artery calcification and mortality of patients with chronic kidney disease and renal transplant","authors":"S. Jovicic-Pavlovic, S. Simić-Ogrizović, Z. Bukumirić, Milena Eric, Natalija Pavlovic, Boba Kotlica, I. Novaković","doi":"10.2298/gensr2201457p","DOIUrl":"https://doi.org/10.2298/gensr2201457p","url":null,"abstract":"Fetuin A is a major systemic inhibitor of vascular calcifications. The aim of this study was to examine association of single nucleotide polymorphisms (SNP) in the gene for fetuin-A with fetuin-A serum levels, coronary arteries calcification (CAC) and mortality in renal transplant (RT) and chronic kidney (CKD) patients. This study included 88 patients (42 stable RT patients at least 6 months after transplantation and 46 CKD patients, stage 2-5 not requiring dialysis) followed five years. Detection and analysis of fetuin A gene polymorphisms in positions C742T (Thr248Met; rs4917) and C766G (Thr256Ser; rs4918) were performed using PCR method. Respondents with allele 742T had at the same time 766G. Combined genotypes TT/GG had lower serum fetuin A levels than CT /CG and CC/CC. Predictors of CAC in univariate analysis were age (p=0,000), serum fetuin-A levels (p=0.011) and rs 4917 polymorphism (p=0.021) while multivariate determined age (p=0.001) and fetuin-A levels (p=0.031). Patients who were homozygous for variant 742T and 766G (combined genotype TT/GG) had lowest survival rate. Our results suggest that allele 742T and 766G in gene for fetuin-A were associated with lower serum fetuin-A levels, higher CAC occurrence and higher mortality rate in RT and CKD patients.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68292860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Celiac disease (CD) is an immune-dependent systemic disorder that occurs in genetically predisposed individuals resulting in damage in the small intestine. It is known that chromatin remodeling, an epigenetic mechanism, is associated with gastrointestinal diseases associated with chronic inflammation. However, no information is available on the link between CD and chromatin remodeling. For this purpose, the expression profiles of chromatin remodeling group genes in children diagnosed with CD according to Marsh classification and HLA profile were evaluated and their relationship with CD was investigated. Endoscopic biopsies embedded in the paraffin block of 40 children with CD diagnosis and 30 healthy children were included in the study. The most common four mutations (DQA1*05, DQB1*02, DQA1*03, and DQB1*03:02) related to CD on human leukocyte antigen (HLA) gene were screened. Intestinal biopsy samples were used for mRNA isolations and cDNA synthesis. Expressions of total seven genes in the chromatin remodeling groups (SWI/SNF Complex Group: ARID1A, Polycomb Group: CTBP1, Nucleosome-Remodeling & Histone Deacetylase (NuRD) Complex Group: MTA1, Chromobox/Heterochromatin Protein 1 (HP1) Homologs Group: CBX3 and CBX7, Homeodomain (PHD) Protein Group: NSD1, Inhibitor of Growth (ING) family group: ING 5) were analyzed by Real-Time qPCR. Data analysis was performed online using the software provided by the manufacturer. Overexpression in ARID1A, CTBP1, and NSD1 genes was detected when the CD group was compared against the control group, however they were not significant (p=0.31, 0.33, and 0.33). When CD group who had diarrhea symptom (typical) were compared to the CD group without diarrhea symptom (atypical), statistically significant under-expression was found in CBX3 and CTBP1 genes (p=0.04 and p=0.004). Statistically significant CTB1 overexpression was detected in Marsh 2 CD cases (p=0.03). In the comparison of HLA DQ2/DQ8 positive CD patient group with the control group, the NSD1, CBX3, and EED (p=0.75, 0.75, and 0.78) genes were over-expressed and the CBX7, MTA1, ARID1A, and CTBP1 genes (p=0.74, 0.75, 0.75, and 0.75) were under-expressed. This is the first study to report that expression of chromatin remodeling genes may have roles in the development and progression of CD. The results of this case-control study are open to confirmation by future studies with larger number of subjects to obtain statistically significant results.
{"title":"Relationship between pediatric celiac disease and chromatin remodeling gene expressions","authors":"S. Boyacıoğlu, M. Caliskan, G. Dogan","doi":"10.2298/gensr2201439b","DOIUrl":"https://doi.org/10.2298/gensr2201439b","url":null,"abstract":"Celiac disease (CD) is an immune-dependent systemic disorder that occurs in genetically predisposed individuals resulting in damage in the small intestine. It is known that chromatin remodeling, an epigenetic mechanism, is associated with gastrointestinal diseases associated with chronic inflammation. However, no information is available on the link between CD and chromatin remodeling. For this purpose, the expression profiles of chromatin remodeling group genes in children diagnosed with CD according to Marsh classification and HLA profile were evaluated and their relationship with CD was investigated. Endoscopic biopsies embedded in the paraffin block of 40 children with CD diagnosis and 30 healthy children were included in the study. The most common four mutations (DQA1*05, DQB1*02, DQA1*03, and DQB1*03:02) related to CD on human leukocyte antigen (HLA) gene were screened. Intestinal biopsy samples were used for mRNA isolations and cDNA synthesis. Expressions of total seven genes in the chromatin remodeling groups (SWI/SNF Complex Group: ARID1A, Polycomb Group: CTBP1, Nucleosome-Remodeling & Histone Deacetylase (NuRD) Complex Group: MTA1, Chromobox/Heterochromatin Protein 1 (HP1) Homologs Group: CBX3 and CBX7, Homeodomain (PHD) Protein Group: NSD1, Inhibitor of Growth (ING) family group: ING 5) were analyzed by Real-Time qPCR. Data analysis was performed online using the software provided by the manufacturer. Overexpression in ARID1A, CTBP1, and NSD1 genes was detected when the CD group was compared against the control group, however they were not significant (p=0.31, 0.33, and 0.33). When CD group who had diarrhea symptom (typical) were compared to the CD group without diarrhea symptom (atypical), statistically significant under-expression was found in CBX3 and CTBP1 genes (p=0.04 and p=0.004). Statistically significant CTB1 overexpression was detected in Marsh 2 CD cases (p=0.03). In the comparison of HLA DQ2/DQ8 positive CD patient group with the control group, the NSD1, CBX3, and EED (p=0.75, 0.75, and 0.78) genes were over-expressed and the CBX7, MTA1, ARID1A, and CTBP1 genes (p=0.74, 0.75, 0.75, and 0.75) were under-expressed. This is the first study to report that expression of chromatin remodeling genes may have roles in the development and progression of CD. The results of this case-control study are open to confirmation by future studies with larger number of subjects to obtain statistically significant results.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68293046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maliheh Mahmoudi, M. Seghatoleslami, G. Moosavi, M. Teimouri
The aim of this study was to investigate the effect of pre-sowing treatments and temperature on Sclerorhachis leptoclada Boiss. seed germination. The following treatments were used), hot water (soaking in water at 70?C and 90?C for 5 second), Moist Chilling (Soaking in water at 2?C for 7,14 and 21day with the temperature gradually falling to room temperature), scarification by 0.2% potassium nitrate (soaking for 24 h and 48 h) and Gibberellic acid (GA: at concentrations of 250, 500 and 750 ppm soaking for 24 h and 48 h) and Distilled Water (control). The percentage of germination, germination rate, germination start (GS), mean germination time (MGT), and germination vigor index were determined as germination indices. Root and shoot length, root fresh weight, root dry weight, shoot fresh weight, shoot dry weight and seedling dry weight were evaluated as growth factors at the end of the incubation period. Second experiment was carried out to study the effect of temperature on seed germination. It was observed that both the physical and chemical scarification methods were effective in enhancing S. leptoclada seed germination and growth. The highest germination rate, percentage of germination and germination vigor index was observed with Moist Chilling (Soaking in water at 2?C for 14 day), 0.2% potassium nitrate (soaking for 48h) and GA (at concentration of 250 ppm soaking for 48h) in compare with control. Also the highest growth factors were found in Moist Chilling (Soaking in water at 2?C for 7day) treated seeds and the lowest occurred in hot water treatments. The other results showed that higher temperature was more effective lower temperature for seed germination.
{"title":"Effects of various dormancy breaking treatment on seed germination in Sclerorhachis leptoclada boiss: an endangered medicinal plant in arid area","authors":"Maliheh Mahmoudi, M. Seghatoleslami, G. Moosavi, M. Teimouri","doi":"10.2298/gensr2202601m","DOIUrl":"https://doi.org/10.2298/gensr2202601m","url":null,"abstract":"The aim of this study was to investigate the effect of pre-sowing treatments and temperature on Sclerorhachis leptoclada Boiss. seed germination. The following treatments were used), hot water (soaking in water at 70?C and 90?C for 5 second), Moist Chilling (Soaking in water at 2?C for 7,14 and 21day with the temperature gradually falling to room temperature), scarification by 0.2% potassium nitrate (soaking for 24 h and 48 h) and Gibberellic acid (GA: at concentrations of 250, 500 and 750 ppm soaking for 24 h and 48 h) and Distilled Water (control). The percentage of germination, germination rate, germination start (GS), mean germination time (MGT), and germination vigor index were determined as germination indices. Root and shoot length, root fresh weight, root dry weight, shoot fresh weight, shoot dry weight and seedling dry weight were evaluated as growth factors at the end of the incubation period. Second experiment was carried out to study the effect of temperature on seed germination. It was observed that both the physical and chemical scarification methods were effective in enhancing S. leptoclada seed germination and growth. The highest germination rate, percentage of germination and germination vigor index was observed with Moist Chilling (Soaking in water at 2?C for 14 day), 0.2% potassium nitrate (soaking for 48h) and GA (at concentration of 250 ppm soaking for 48h) in compare with control. Also the highest growth factors were found in Moist Chilling (Soaking in water at 2?C for 7day) treated seeds and the lowest occurred in hot water treatments. The other results showed that higher temperature was more effective lower temperature for seed germination.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68294096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Healy Arora, S. Jindal, Abhishek Sharma, Rupeet Gill, N. Chawla
The current study in tomato (Solanum lycopersicum L.) was conducted at PAU, Ludhiana with the objective of developing hybrids possessing combined resistance to late blight and leaf curl virus diseases along with desirable horticultural characteristics. The experimental material which included 32 F1 hybrids (developed by line ? tester method), 12 parental lines (8 lines and 4 testers; including susceptible check Punjab Chhuhara) and standard check NS-524 were all planted in randomized complete block design with three replications. The values of ?2SCA/?2GCA were more than unity for all the traits except average fruit weight and ascorbic acid content, indicating the predominance of non-additive gene effects. Cross combinations CLN-154 ? LBR-12 and CLN-154 ? LBR-21 recorded significant heterosis over better parent and check for fruit yield and other quality characteristics. Artificial and natural screening was performed for all the experimental material against late blight and leaf curl virus diseases respectively. Out of 32 hybrids, crosses namely CLN-154? LBR-12, CLN-154 ? LBR-21, PVB-1 ? LBR-10, PVB-4 ? LBR-12 and CLN-104 ? LBR-10 were identified for combined disease resistance against late blight and leaf curl virus, in relation to desirable horticultural characteristics particularly fruit yield, average fruit weight, pericarp thickness, dry matter, titrable acidity and ascorbic acid content with fair amount of heterosis. Hence, the hybrids which displayed good potential in yield with acceptable performance of qualitative traits, along with combined disease resistance could be utilized for commercial exploitation.
{"title":"Development and evaluation of hybrids resistant to late blight and leaf curl virus diseases in tomato","authors":"Healy Arora, S. Jindal, Abhishek Sharma, Rupeet Gill, N. Chawla","doi":"10.2298/gensr2202801a","DOIUrl":"https://doi.org/10.2298/gensr2202801a","url":null,"abstract":"The current study in tomato (Solanum lycopersicum L.) was conducted at PAU, Ludhiana with the objective of developing hybrids possessing combined resistance to late blight and leaf curl virus diseases along with desirable horticultural characteristics. The experimental material which included 32 F1 hybrids (developed by line ? tester method), 12 parental lines (8 lines and 4 testers; including susceptible check Punjab Chhuhara) and standard check NS-524 were all planted in randomized complete block design with three replications. The values of ?2SCA/?2GCA were more than unity for all the traits except average fruit weight and ascorbic acid content, indicating the predominance of non-additive gene effects. Cross combinations CLN-154 ? LBR-12 and CLN-154 ? LBR-21 recorded significant heterosis over better parent and check for fruit yield and other quality characteristics. Artificial and natural screening was performed for all the experimental material against late blight and leaf curl virus diseases respectively. Out of 32 hybrids, crosses namely CLN-154? LBR-12, CLN-154 ? LBR-21, PVB-1 ? LBR-10, PVB-4 ? LBR-12 and CLN-104 ? LBR-10 were identified for combined disease resistance against late blight and leaf curl virus, in relation to desirable horticultural characteristics particularly fruit yield, average fruit weight, pericarp thickness, dry matter, titrable acidity and ascorbic acid content with fair amount of heterosis. Hence, the hybrids which displayed good potential in yield with acceptable performance of qualitative traits, along with combined disease resistance could be utilized for commercial exploitation.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68294269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The neurobiology of obsessive-compulsive disorder (OCD) is evidenced by a strong demonstration of malfunctions in the serotonergic and dopaminergic system. Recently, glial cell line-derived neurotrophic factor (GDNF) gene polymorphisms have been emphasized in psychiatric diseases and treatment strategies that have been tried to be developed in this regard. In the literature, there are several studies investigating the relationship between GDNF gene polymorphisms and psychiatric diseases excluding OCD. Therefore, this study aimed to compare the symptomatology and GDNF gene polymorphisms in early and late-onset OCD patients. For this purpose, patients diagnosed with OCD according to DSM-V diagnostic criteria in structured clinical interviews were grouped as early and late-onset based on the age of initiation. DNA was isolated from blood samples collected from 140 subjects (70 OCD and 70 healthy controls) in EDTA tubes, and rs2910702, rs3096140, and rs3812047 polymorphisms in GDNF gene were examined by Real-Time PCR. No significant correlation was detected between GDNF and the rs2910702, rs3096140, and rs3812047 polymorphisms in early and late-onset OCD subjects (P>0.05). Failure to detect correlations between OCD and GDNF gene polymorphisms might be due to the variable expression pattern of the GDNF gene in different tissues and pathologies. Therefore, future studies might be improved by including a larger group of patients and examining a wider range of tissues for the expression pattern of GDNF.
{"title":"GDNF rs2910702, rs3096140, and rs3812047 polymorphisms in obsessive compulsive disorder: Preliminary study","authors":"Seda Orenay-Boyacioglu, M. Caliskan, Ayse Dondu","doi":"10.2298/gensr2202817o","DOIUrl":"https://doi.org/10.2298/gensr2202817o","url":null,"abstract":"The neurobiology of obsessive-compulsive disorder (OCD) is evidenced by a strong demonstration of malfunctions in the serotonergic and dopaminergic system. Recently, glial cell line-derived neurotrophic factor (GDNF) gene polymorphisms have been emphasized in psychiatric diseases and treatment strategies that have been tried to be developed in this regard. In the literature, there are several studies investigating the relationship between GDNF gene polymorphisms and psychiatric diseases excluding OCD. Therefore, this study aimed to compare the symptomatology and GDNF gene polymorphisms in early and late-onset OCD patients. For this purpose, patients diagnosed with OCD according to DSM-V diagnostic criteria in structured clinical interviews were grouped as early and late-onset based on the age of initiation. DNA was isolated from blood samples collected from 140 subjects (70 OCD and 70 healthy controls) in EDTA tubes, and rs2910702, rs3096140, and rs3812047 polymorphisms in GDNF gene were examined by Real-Time PCR. No significant correlation was detected between GDNF and the rs2910702, rs3096140, and rs3812047 polymorphisms in early and late-onset OCD subjects (P>0.05). Failure to detect correlations between OCD and GDNF gene polymorphisms might be due to the variable expression pattern of the GDNF gene in different tissues and pathologies. Therefore, future studies might be improved by including a larger group of patients and examining a wider range of tissues for the expression pattern of GDNF.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68294329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Popović, V. Daničić, J. Milovanović, A. Lučić, L. Rakonjac, S. Mladenović-Drinić, D. Ristić
The paper presents the results of the genetic diversity analysis of Sessile oak populations from the area of outstanding natural beauty (AONB) "Avala" which were obtained using SSR markers. Genomic DNA was isolated from leaf tissue of 50 test trees from two populations. Genotyping was performed using microsatellite markers QpZAG110, QpZAG15, QpZAG1/2, QpZAG3/64, QpZAG36, QpZAG1/5, and QrZAG108. All loci were polymorphic with the high mean value of PIC (0.934). The total number of alleles determined in the studied population was 127. The range of alleles varies from 15 (QpZAG1/5, QpZAG1/2) to 23 (QpZAG110) with an average of 18.14 alleles per locus. The number of effective alleles ranges from 8.273 (QpZAG1/5) to 13.830 (QrZAG108). The mean value of the gene flow (Nm) was 8.522 with a range from 5.548 to 14.876. Overall genetic diversity was high (He = 0.909) and ranged from 0.879 to 0.928. Due to the excess of homozygotes observed at most loci, a significant inbreeding coefficient was detected (Fis = 0.796). The Analysis of Molecular Variance (AMOVA) confirmed that genetic diversity was more pronounced within populations (77.5%) than between them (1.6%). The average allele frequency (Q) of the studied populations shows that the individuals originate from two or more populations. The obtained results can be used for the adoption of appropriate plans for the management of protected natural resources and the management of this ecologically and economically important tree species. Also, the obtained results enable the adoption of the necessary measures for the conservation of sessile oak genetic resources by in-situ and ex-situ methods. Based on the research results, the use of this important species can be recommended for its reintroduction in optimal microclimatic conditions, as well as in the selection of the best individuals for the reintroduction.
{"title":"Genetic structure of Sessil oak (Quercus petraea (matt.) liebl) from the area of outstanding natural beauty “Avala“","authors":"V. Popović, V. Daničić, J. Milovanović, A. Lučić, L. Rakonjac, S. Mladenović-Drinić, D. Ristić","doi":"10.2298/gensr2202841p","DOIUrl":"https://doi.org/10.2298/gensr2202841p","url":null,"abstract":"The paper presents the results of the genetic diversity analysis of Sessile oak populations from the area of outstanding natural beauty (AONB) \"Avala\" which were obtained using SSR markers. Genomic DNA was isolated from leaf tissue of 50 test trees from two populations. Genotyping was performed using microsatellite markers QpZAG110, QpZAG15, QpZAG1/2, QpZAG3/64, QpZAG36, QpZAG1/5, and QrZAG108. All loci were polymorphic with the high mean value of PIC (0.934). The total number of alleles determined in the studied population was 127. The range of alleles varies from 15 (QpZAG1/5, QpZAG1/2) to 23 (QpZAG110) with an average of 18.14 alleles per locus. The number of effective alleles ranges from 8.273 (QpZAG1/5) to 13.830 (QrZAG108). The mean value of the gene flow (Nm) was 8.522 with a range from 5.548 to 14.876. Overall genetic diversity was high (He = 0.909) and ranged from 0.879 to 0.928. Due to the excess of homozygotes observed at most loci, a significant inbreeding coefficient was detected (Fis = 0.796). The Analysis of Molecular Variance (AMOVA) confirmed that genetic diversity was more pronounced within populations (77.5%) than between them (1.6%). The average allele frequency (Q) of the studied populations shows that the individuals originate from two or more populations. The obtained results can be used for the adoption of appropriate plans for the management of protected natural resources and the management of this ecologically and economically important tree species. Also, the obtained results enable the adoption of the necessary measures for the conservation of sessile oak genetic resources by in-situ and ex-situ methods. Based on the research results, the use of this important species can be recommended for its reintroduction in optimal microclimatic conditions, as well as in the selection of the best individuals for the reintroduction.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68294460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Ristanović, Z. Ilic, V. Caro-Petrovic, Vukasin Stefanovic, Irina Sicheva, Natasa Perovic
The dynamics of increasing of bodyweight of lambs is conditioned by genetic factors, environmental factors and their interaction. The aim of this work was study variability of bodyweight of lambs related to type of birth of lambs in sheep genotype of four different strains of pramenka type and four growth stage: at the birth time, at the 30, 60 and 90 days of age after birth. For this investigation used lambs of four strains (Svrljiski, Pirotski, Sjeni?ki, Sarplaninski) of sheep type Pramenka (450 lambs per strain) for study dinamics of bodyweight at the time of birth, 30, 60 and 90 days after birth during three years of experiment (2011-2013). The results showed that the bodyweight of lambs born singles was higher than bodyweight of lambs born twins in studied four sheep genotypes i.e. Pirotski (4.00 kg : 3.40 kg), Svrljiski (3.90 kg : 3.79 kg), Sjeni?ki (3.73 kg : 3.61 kg), Sarplaninski (3.81 kg : 3.74 kg). The differences of bodyweight between lambs born singles and twins was significant for genotypes Pirot pramenka and Svrljig pramenka, on the level of significance for Sjenica pramenka and was not significant in Sarplanina pramenka. At the age of 30 days, the influence of birth type on the bodyweight of lambs was not statistically significant in any of the examined strains. The difference of bodyweight between singles and twins was statistically significant at the 60 days old of lambs in Svrljig pramenka and in lambs at 90 days old of Svrljig pramenka. On the base of results is established that type of born associated with significant differences of bodyweight at the birht time expressed later at the 60 and 90 day old lambs.
{"title":"Influence of birth type on body weight of lambs from birth to weaning in various strains of sheep pramenka","authors":"B. Ristanović, Z. Ilic, V. Caro-Petrovic, Vukasin Stefanovic, Irina Sicheva, Natasa Perovic","doi":"10.2298/gensr2202749r","DOIUrl":"https://doi.org/10.2298/gensr2202749r","url":null,"abstract":"The dynamics of increasing of bodyweight of lambs is conditioned by genetic factors, environmental factors and their interaction. The aim of this work was study variability of bodyweight of lambs related to type of birth of lambs in sheep genotype of four different strains of pramenka type and four growth stage: at the birth time, at the 30, 60 and 90 days of age after birth. For this investigation used lambs of four strains (Svrljiski, Pirotski, Sjeni?ki, Sarplaninski) of sheep type Pramenka (450 lambs per strain) for study dinamics of bodyweight at the time of birth, 30, 60 and 90 days after birth during three years of experiment (2011-2013). The results showed that the bodyweight of lambs born singles was higher than bodyweight of lambs born twins in studied four sheep genotypes i.e. Pirotski (4.00 kg : 3.40 kg), Svrljiski (3.90 kg : 3.79 kg), Sjeni?ki (3.73 kg : 3.61 kg), Sarplaninski (3.81 kg : 3.74 kg). The differences of bodyweight between lambs born singles and twins was significant for genotypes Pirot pramenka and Svrljig pramenka, on the level of significance for Sjenica pramenka and was not significant in Sarplanina pramenka. At the age of 30 days, the influence of birth type on the bodyweight of lambs was not statistically significant in any of the examined strains. The difference of bodyweight between singles and twins was statistically significant at the 60 days old of lambs in Svrljig pramenka and in lambs at 90 days old of Svrljig pramenka. On the base of results is established that type of born associated with significant differences of bodyweight at the birht time expressed later at the 60 and 90 day old lambs.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68294539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Stupar, Željko Savković, Katarina Breka, I. Krizmanić, S. Stamenković, J. Vukojevic, M. Ljaljevic-Grbic
A rare basidiomycete Quambalaria cyanescens, documented so far on various substrates worldwide, was isolated from the skin of edible frog (Pelophylax esculentus) captured in South Banat. The fungal identification was based on sequencing of ITS region and BLAST analyses. The presence of Q. cyanescens in the amphibian skin microbiome is not only the first finding of this fungus in Serbia but also the recording of new ecological habitat for this rare species of micromycetes. Phylogenetic analyses revealed the high similarity of isolate in this study with foliar pathogens of Eucalyptus in Australia.
{"title":"New record for mycobiota of Serbia: A rare fungus Quambalaria cyanescens found in Pelophylax esculentus (Anura) skin microbiome","authors":"M. Stupar, Željko Savković, Katarina Breka, I. Krizmanić, S. Stamenković, J. Vukojevic, M. Ljaljevic-Grbic","doi":"10.2298/gensr2203101s","DOIUrl":"https://doi.org/10.2298/gensr2203101s","url":null,"abstract":"A rare basidiomycete Quambalaria cyanescens, documented so far on various substrates worldwide, was isolated from the skin of edible frog (Pelophylax esculentus) captured in South Banat. The fungal identification was based on sequencing of ITS region and BLAST analyses. The presence of Q. cyanescens in the amphibian skin microbiome is not only the first finding of this fungus in Serbia but also the recording of new ecological habitat for this rare species of micromycetes. Phylogenetic analyses revealed the high similarity of isolate in this study with foliar pathogens of Eucalyptus in Australia.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68295723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The major objective in pepper breeding programs is to generate high yielding novel varieties resistant to pests and diseases, tolerant to abiotic stress conditions with improved fruit quality traits including capsaicin content. Germplasm collections are important sources of variability for breeding studies. Molecular markers are important tools to evaluate genetic relationships among germplasm collections. Moreover, markers are used to select the genotypes conferring the desired traits via marker-assisted selection (MAS) as a powerful approach accelerating breeding programs. In the current study, 56 pepper genotypes selected among 313 pepper genotypes collected from K?r?ehir province in the Central Anatolian region of Turkey according to their agronomic and morphological characteristics were used for molecular assays. Six SSR markers two of which were linked to fruit morphology were selected to characterize pepper genotypes according to their high polymorphism information content. Three SCAR markers associated with capsaicinoid synthesis and resistance to Phytophtora capsici (Phyto.5.2) in pepper were used to assess pungency and resistance among genotypes. According to the results obtained with SSR markers, the total number of alleles ranged from 1 to 8 among genotypes. The most polymorphic SSR markers were CaeMS015 and CAMS452 within the pepper population. The genetic distance among genotypes was determined ranging between 0.75-1.00. The segregation of the SCAR marker BF6-BF8 linked to pungency in pepper was determined relative to pungent and sweet reference cultivars. The OP004.717 SCAR marker linked to Phytophtora capsici was tested among genotypes relative to CM334 pepper variety, known as a source of resistance to Phytophtora.
{"title":"Genetic characterization of pepper (Capsicum annuum L.) genotypes from central Anatolia with SSR and SCAR markers","authors":"H. Başak, G. Sarıkamış, G. Çakirer, Alev Ateş","doi":"10.2298/gensr2203171b","DOIUrl":"https://doi.org/10.2298/gensr2203171b","url":null,"abstract":"The major objective in pepper breeding programs is to generate high yielding novel varieties resistant to pests and diseases, tolerant to abiotic stress conditions with improved fruit quality traits including capsaicin content. Germplasm collections are important sources of variability for breeding studies. Molecular markers are important tools to evaluate genetic relationships among germplasm collections. Moreover, markers are used to select the genotypes conferring the desired traits via marker-assisted selection (MAS) as a powerful approach accelerating breeding programs. In the current study, 56 pepper genotypes selected among 313 pepper genotypes collected from K?r?ehir province in the Central Anatolian region of Turkey according to their agronomic and morphological characteristics were used for molecular assays. Six SSR markers two of which were linked to fruit morphology were selected to characterize pepper genotypes according to their high polymorphism information content. Three SCAR markers associated with capsaicinoid synthesis and resistance to Phytophtora capsici (Phyto.5.2) in pepper were used to assess pungency and resistance among genotypes. According to the results obtained with SSR markers, the total number of alleles ranged from 1 to 8 among genotypes. The most polymorphic SSR markers were CaeMS015 and CAMS452 within the pepper population. The genetic distance among genotypes was determined ranging between 0.75-1.00. The segregation of the SCAR marker BF6-BF8 linked to pungency in pepper was determined relative to pungent and sweet reference cultivars. The OP004.717 SCAR marker linked to Phytophtora capsici was tested among genotypes relative to CM334 pepper variety, known as a source of resistance to Phytophtora.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68295747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Safflower is an annual oilseed crop which has healthy edible oil containing high amount of unsaturated fatty acids in the world. In this study, we investigated safflower registered cultivars of Turkey (4), genotypes retrieved from USDA (10) and their hybrids (45) for genetic variation using 10 simple sequence repeat (SSR) loci. Genetic diversity calculated registered cultivars, genotypes and hybrids were as follows: mean number of alleles (4.67), expected heterozygosity (0.680), average effective number of alleles (3.172), and polymorphism information content (0.664). The dendrogram analysis revealed at least four possible major clusters in the parents and hybrids. High level of genetic diversity explained between the populations and Fst calculate (0.593) suggested that the clusters were differentiated to each other. Registered safflower cultivars of Turkey were distributed across all four clusters and the accessions from USA were defined in most of the clusters. The dendrogram based method analysis revealed two major clusters which corresponded to spiny and spineless safflower genotypes. It was suggested that the studied 6 SSR markers could be utilized for safflower breeding studies based on molecular analysis.
{"title":"The genetic diversity of safflower (Carthamus tinctorius L.) genotypes developed by hybridization using SSR markers","authors":"E. Culpan, B. Arslan","doi":"10.2298/gensr2203193c","DOIUrl":"https://doi.org/10.2298/gensr2203193c","url":null,"abstract":"Safflower is an annual oilseed crop which has healthy edible oil containing high amount of unsaturated fatty acids in the world. In this study, we investigated safflower registered cultivars of Turkey (4), genotypes retrieved from USDA (10) and their hybrids (45) for genetic variation using 10 simple sequence repeat (SSR) loci. Genetic diversity calculated registered cultivars, genotypes and hybrids were as follows: mean number of alleles (4.67), expected heterozygosity (0.680), average effective number of alleles (3.172), and polymorphism information content (0.664). The dendrogram analysis revealed at least four possible major clusters in the parents and hybrids. High level of genetic diversity explained between the populations and Fst calculate (0.593) suggested that the clusters were differentiated to each other. Registered safflower cultivars of Turkey were distributed across all four clusters and the accessions from USA were defined in most of the clusters. The dendrogram based method analysis revealed two major clusters which corresponded to spiny and spineless safflower genotypes. It was suggested that the studied 6 SSR markers could be utilized for safflower breeding studies based on molecular analysis.","PeriodicalId":50423,"journal":{"name":"Genetika-Belgrade","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68295957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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