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Isolation And Identification Of Staphylococcus Aureus And Candida SPP From Throat Infections 咽喉感染中金黄色葡萄球菌和 SPP 念珠菌的分离与鉴定
Pub Date : 2024-01-29 DOI: 10.58806/ijhmr.2024.v3i1n06
Azhar Jasim Mohsin, Nadia Habeeb Sarhan, H. G. Idreess
This research aimed to isolate and identify Staphylococcus aureus and Candida species from throat infections, as well as assess their antibiotic resistance. Samples were collected from both infected patients and healthy individuals, encompassing various ages and genders, in hospitals of Najaf province between December 2022 and March 2023. The findings revealed that S. aureus constituted 36.10% of isolates, followed by S. warneri at 22.81%, and S. lugdunensis at 15.29%. Notably, S. aureus isolates displayed 100% resistance to Penicillin (P), Aztreonam (ATM), and Oxacillin (OX), Amoxicillin (AMC) and Tetracycline (TE) exhibiting resistance rates of 81.95% and 78.19%, respectively. The study concluded that S. aureus was the predominant strain, followed by S. warneri. Antibiotic susceptibility testing on all S. aureus isolates (n=21) was conducted using seven selected antibiotics. Candida species identified included Candida albicans, Candida parapsilosis, and Candida glabrata, with maximum resistance observed against fluconazole (FLU) and polymyxin B (PB), followed by miconazole (MCL).
这项研究旨在从咽喉感染中分离和鉴定金黄色葡萄球菌和白色念珠菌,并评估它们的抗生素耐药性。研究人员于 2022 年 12 月至 2023 年 3 月期间在纳杰夫省的医院从感染患者和健康人(包括不同年龄和性别)身上采集了样本。研究结果显示,金黄色葡萄球菌占分离菌株的 36.10%,其次是华奈氏菌(22.81%)和卢格敦氏菌(15.29%)。值得注意的是,分离出的金黄色葡萄球菌对青霉素(P)、阿曲南(ATM)和奥沙西林(OX)、阿莫西林(AMC)和四环素(TE)显示出 100%的耐药性,耐药率分别为 81.95% 和 78.19%。研究得出结论,金黄色葡萄球菌是主要菌株,其次是华奈氏菌。使用七种选定的抗生素对所有金黄色葡萄球菌分离株(n=21)进行了抗生素敏感性测试。鉴定出的念珠菌包括白色念珠菌、副丝状念珠菌和光滑念珠菌,对氟康唑(FLU)和多粘菌素 B(PB)的耐药性最强,其次是咪康唑(MCL)。
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引用次数: 0
Simulation Of Orange Fruit Use And Small Group Discussions In A Village With Extreme Poverty To Mitigate Anemia In Pregnant Women: A Community Program Report In Indonesia 在一个极端贫困的村庄模拟使用橙果和小组讨论,以减轻孕妇贫血症:印度尼西亚社区项目报告
Pub Date : 2024-01-18 DOI: 10.58806/ijhmr.2024.v3i1n04
Astri Nurdiana, Eni Kurniati, Hansrizka Raisna, Ketie Salmah Ginarti, Riska Setiawati, Wina Ramanda Sari, Yulfitriani Solehati, Rukmaini Rukmaini
Introduction: Haemorrhage remains one of the leading causes of maternal mortality in Indonesia. One significant factor contributing to bleeding is the prevalence of anemia among pregnant women. Early detection of anemia is crucial in preventing this condition, but the limited knowledge among pregnant women can impede successful treatment of anemia in Indonesia. Objective: The purpose of this community service project is to enhance pregnant women’s understanding of anemia during pregnancy and enhance their mindset so that they recognize the significance of avoiding and treating anemia during pregnancy. Research Method: The study involved conducting a simulation that utilized citrus fruit and organizing small group discussions about anemia. The participants were 30 pregnant women residing in impoverished areas in the Karawang district. Results: The minimal knowledge score showed a significant rise between before and after the activity, rising from a minimum score of 7 to a minimum score of 13. Similarly, the maximum knowledge score climbed from 80 to 100. The majority of respondents expressed a strong consensus that anemia poses a significant risk to pregnancy. Consequently, respondents also strongly concurred on the need for concerted measures to address this issue. To prevent anemia, it is important to take iron supplement tablets and maintain a diet that is rich in nutritious food. Conclusion: Engaging in positive activities outside of pregnancy check-ups with healthcare professionals can serve as a platform for receiving support and fostering mutual sharing among pregnant women. This has the potential to enhance the knowledge and attitudes of pregnant women towards preventing anemia throughout pregnancy.
导言:出血仍是印度尼西亚孕产妇死亡的主要原因之一。导致出血的一个重要因素是孕妇普遍贫血。及早发现贫血对预防这种情况至关重要,但在印度尼西亚,孕妇对贫血的了解有限,这可能会阻碍贫血的成功治疗。目标:本社区服务项目旨在加强孕妇对孕期贫血的了解,提高她们的思想认识,使她们认识到避免和治疗孕期贫血的意义。研究方法:研究内容包括利用柑橘类水果进行模拟,并组织关于贫血的小组讨论。参与者为居住在卡拉旺地区贫困地区的 30 名孕妇。结果最低知识点得分在活动前后有明显上升,从最低的 7 分上升到最低的 13 分。同样,最高知识分数也从 80 分上升到 100 分。大多数受访者对贫血会给怀孕带来重大风险这一观点达成了强烈共识。因此,受访者也强烈认为有必要采取协调一致的措施来解决这一问题。为了预防贫血,服用铁质补充剂和保持富含营养的饮食非常重要。结论与医护人员一起参与孕期检查以外的积极活动,可作为孕妇获得支持和促进相互分享的平台。这有可能增强孕妇在整个孕期预防贫血的知识和态度。
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引用次数: 0
Relationship Between Stress And Alpha Amylase Levels Among Presenters 压力与演讲者α-淀粉酶水平之间的关系
Pub Date : 2024-01-16 DOI: 10.58806/ijhmr.2024.v3i1n03
Ali Majid Hassan, Ali J Eidan, Murtadha Kanim Adea
Background: Stress occurs when the internal homeostasis is disrupted by external environmental stressors. Salivary Alpha Amylase (sAA) levels are increased in response to stress, and it is frequently used as a biomarker of stress. Aims: To determine the relationship between stress and alpha-amylase levels among presenters by the difference in levels scored in pre and post presentation. Material and Methods: A cross-sectional descriptive study was conducted to achieve the objectives of the study and was achieved to determine the association between stress and salivary alpha amylase levels for presenters during the period from 10th. Jan, 2022 to Jan, 20th. Mar, 2022. The study was conducted by selecting a typical form of non-probability (Purposive) sample. The size of the sample of 70 participants was included in the study. Results: The relationship between stress and the level of alpha amylase concentration was low in post presentation in comparison with pre presentation (0.11±0.03) and (0.13±0.02) respectively. This result was statistically highly significant according to paired t-test and the P-value was < 0.001. Furthermore, the level of Alpha amylase activity was low in post presentation in comparison with pre presentation (289658.5±76490.7) and (321693.9±52219.2) respectively. This result was statistically highly significant according to paired one group t-test and the P-value was<=0.0001. Conclusion: It was concluded Stress was elevated in the post-presentation in compare with the pre-presentation. and the levels of alpha amylase were affected by the presenters’ age groups.
背景:当体内平衡受到外部环境压力的破坏时,就会产生压力。唾液中的α-淀粉酶(sAA)水平会随着压力的增加而升高,经常被用作压力的生物标志物。目的:通过演讲前和演讲后α-淀粉酶水平的差异,确定演讲者压力与α-淀粉酶水平之间的关系。材料与方法为实现研究目标,我们进行了一项横断面描述性研究,以确定 2022 年 1 月 10 日至 2020 年 1 月 20 日期间演讲者的压力与唾液α-淀粉酶水平之间的关系。2022年1月10日至1月20日。2022年3月。研究采用了典型的非概率(有目的)抽样形式。参与研究的样本人数为 70 人。研究结果与发病前(0.11±0.03)和(0.13±0.02)相比,发病后(0.11±0.03)和(0.13±0.02)的压力与α-淀粉酶浓度水平之间的关系较低。根据配对 t 检验,这一结果具有高度统计学意义,且 P 值小于 0.001。此外,与发病前(289658.5±76490.7)和(321693.9±52219.2)相比,发病后的α-淀粉酶活性水平较低。根据配对单组 t 检验,该结果具有高度统计学意义,P 值<=0.0001。结论α-淀粉酶的水平受到演讲者年龄组的影响。
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引用次数: 0
Identification Of Locally Transmitted COVID-19 Spatial Clusters And Hotspots 识别本地传播的 COVID-19 空间集群和热点
Pub Date : 2024-01-16 DOI: 10.58806/ijhmr.2024.v3i1n02
Thi-Quynh Nguyen, Thi-Hien Cao
Background: The coronavirus disease 2019 (COVID-19) is an emerging and rapidly evolving profound pandemic, which causes severe acute respiratory syndrome and results in significant case fatality around the world. We conducted this study to identify locally transmitted COVID-19 spatial clusters and hotspots in this phrase of the fourth wave in Vietnam. Data used and Methods: A total of 9,192 locally transmitted cases confirmed in this phrase in the fourth wave were used in study. Global and local Moran’s I and Getis-Ord’s G_i^* statistics were employed to identify spatial autocorrelation and hotspots of COVID-19 cases. Results: It was found that global Moran’s I statistic indicates a robust spatial autocorrelation of COVID-19 cases. Local Moran’s I statistic successfully identified three high-high spatial clusters of COVID-19 cases in Bac Giang (5,083 cases), Bac Ninh (1,407 cases), and Hanoi (464 cases). In addition, hotspots of COVID-19 cases were mainly detected in Bac Giang (5,083 cases), Bac Ninh (1,470 cases), Hanoi (464 cases), Hai Duong (51 cases), and Thai Nguyen (7 cases). Conclusion: The results of this work offer new perspectives on the geostatistical analysis of COVID-19 clusters and hotspots, which could help policy planners anticipate the dynamics of spatiotemporal transmission and develop critical control measures for SARS-CoV-2 in Vietnam. Future pandemics and epidemics can be avoided and controlled with the help of geospatial analysis techniques.
背景:2019年冠状病毒病(COVID-19)是一种新出现且迅速发展的深度流行病,会导致严重急性呼吸系统综合征,并在全球范围内造成大量病例死亡。我们开展了这项研究,以确定在越南第四波这一短语中当地传播的 COVID-19 空间集群和热点。使用的数据和方法:研究共使用了 9,192 例在第四波这一短语中确诊的本地传播病例。采用全局和局部 Moran's I 和 Getis-Ord's G_i^* 统计法确定 COVID-19 病例的空间自相关性和热点。结果发现结果发现,全局莫兰 I 统计表明 COVID-19 病例具有稳健的空间自相关性。地方莫兰 I 统计成功地识别出 COVID-19 病例的三个高空间集群,分别位于北江省(5,083 例)、北宁省(1,407 例)和河内市(464 例)。此外,COVID-19 病例的热点地区主要集中在北江省(5 083 例)、北宁省(1 470 例)、河内省(464 例)、海阳省(51 例)和太原省(7 例)。结论这项工作的结果为 COVID-19 群集和热点的地理统计分析提供了新的视角,有助于政策规划者预测时空传播的动态,并为越南的 SARS-CoV-2 制定关键的控制措施。在地理空间分析技术的帮助下,可以避免和控制未来的流行病和疫情。
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引用次数: 0
Physicochemical Determination Of Ethyl Acetate Extract And N-Hexane Extract Of Azadiractha Indica A. Juss Leaves 乙酸乙酯提取物和 N-己烷提取物的理化测定
Pub Date : 2024-01-10 DOI: 10.58806/ijhmr.2024.v3i1n01
Yuri Pratiwi Utami, Nurzadrina Wahyuddin, Ainun Jariah, Rahma Mustari, Imelda Desi
Background: Neem (Azadiractha indica A. Juss) as a tropical and subtropical plant has been widely used in traditional medicine since prehistoric times. Several biological activities, such as antibacterial, anti-inflammatory and antioxidant properties, have been demonstrated in Neem extract. Aim: The study aims to research was carried out on the physicochemical properties of neem leaves (Azadirachta indica A. Juss) using ethyl acetate and n-hexane as solvents. Methode: In this study, Azadiractha indica A. Juss leaves was extracted using a maceration method with two fluids namely ethyl acetate a n-hexane. The extract based on six parameters from determining physicochemical properties, namely % yield, organoleptic, determination of dissolved compound levels, phytochemical screening, determination of drying Shrinkage and determination of specific weight. Result : The results of this study showed, the yield obtained for each solvent is 6.218% ethyl acetate extract and 0.968 % n-hexane extract. Organoleptic examination (form, colour, smell and flavor) for ethyl acetate extract was thick, blackish green, distinctive smell and bitter, and than n-hexan extract was thick, brownish yellow, odorless and bitter. In the ethyl acetate extract, the levels of compounds dissolved in water and ethanol solvents were 16.84 % ± 0.061 and 68.29 % ± 0.042 respectively. The levels of compounds in the n-hexane extract dissolved in water and ethanol were 4.26% ± 0.064 and 23.18% ± 0.049, respectively. The results of the phytochemical screening for ethyl acetate extract of neem leaves are alkaloids and flavonoids. Meanwhile, n-Hexane extract from neem leaves contains alkaloids and terpenoids. The drying loss values obtained from ethyl acetate extract and n-Hexan extract of neem leaves were 4.748% ± 0.257 and 5.219% ± 0.089. Specific gravity obtained from The dilution of ethyl acetate extract and n-hexane extract of neem leaves was 1.0389 g/mL ± 0.039 and 1.0289 g/mL ± 0.038. Conclusion: This study showed that variations in solvents in extraction can affect the physicochemical properties. The extract with ethyl acetate solvent showed better physicochemical properties where the extract obtained in % yield, soluble compound content, phytochemical screening, determination of drying Shrinkage and determination of specific weight was better compared to the extract with n-hexane.
背景:印楝(Azadiractha indica A. Juss)是一种热带和亚热带植物,自史前时代以来就被广泛用于传统医学。印楝提取物具有多种生物活性,如抗菌、消炎和抗氧化特性。目的:本研究以乙酸乙酯和正己烷为溶剂,对印楝叶(Azadirachta indica A. Juss)的理化性质进行了研究。方法:本研究采用浸渍法,用乙酸乙酯和正己烷两种溶剂萃取苦楝叶。提取物根据六项参数确定理化性质,即产量百分比、感官、溶解化合物含量测定、植物化学筛选、干燥收缩率测定和比重测定。结果:研究结果表明,每种溶剂的提取率分别为 6.218%(乙酸乙酯提取物)和 0.968%(正己烷提取物)。乙酸乙酯提取物的感官检查(形、色、嗅、味)为粘稠、黑绿色、有特殊气味和苦味,而正己烷提取物则为粘稠、棕黄色、无味和苦味。在乙酸乙酯提取物中,溶于水和乙醇溶剂的化合物含量分别为 16.84 % ± 0.061 和 68.29 % ± 0.042。正己烷提取物溶于水和乙醇的化合物含量分别为 4.26% ± 0.064 和 23.18% ± 0.049。苦楝叶乙酸乙酯提取物的植物化学筛选结果为生物碱和黄酮类化合物。而楝树叶的正己烷提取物则含有生物碱和萜类化合物。楝树叶乙酸乙酯提取物和正己烷提取物的干燥损耗值分别为 4.748% ± 0.257 和 5.219% ± 0.089。楝树叶乙酸乙酯提取物和正己烷提取物的稀释比重分别为 1.0389 g/mL ± 0.039 和 1.0289 g/mL ± 0.038。结论这项研究表明,萃取溶剂的变化会影响理化性质。乙酸乙酯溶剂提取物显示出更好的理化性质,与正己烷提取物相比,乙酸乙酯提取物在收率%、可溶性化合物含量、植物化学筛选、干燥收缩率测定和比重测定方面都更好。
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International Journal Of Health &amp; Medical Research
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