Currently, the recently erected genus Crenubiotus (Adorybiotidae, Macrobiotoidea) includes only three species, all of which are characterised by dentate lunulae and cuticular tubercules organised in the band in the dorso-caudal part of the body. By means of integrative taxonomy, we describe a fourth species of the genus: Crenubiotus salishani sp. nov., from Salt Spring Island in British Columbia, Canada. The new species has been found in the moss growing on rock and differs from the other species in the genus due to the presence of a median anterior mucrone in the third band of the oral cavity armature (OCA) and by the presence of evident thickenings on the eggshell connecting the neighbouring processes. This finding highlights the importance of continuing to study tardigrade biodiversity, even in already explored areas, and how an integrative approach is fundamental to achieving a reliable measure of biodiversity.
{"title":"A New Species of the GenusCrenubiotus (Tardigrada: Eutardigrada: Adorybiotidae) from Salt Spring Island, Strait of Georgia, British Columbia (Canada)","authors":"M. Vecchi, Henry Choong, S. Calhim","doi":"10.3409/fb_70-3.11","DOIUrl":"https://doi.org/10.3409/fb_70-3.11","url":null,"abstract":"Currently, the recently erected genus Crenubiotus (Adorybiotidae, Macrobiotoidea) includes only three species, all of which are characterised by dentate lunulae and cuticular tubercules organised in the band in the dorso-caudal part of the body. By means of integrative taxonomy,\u0000 we describe a fourth species of the genus: Crenubiotus salishani sp. nov., from Salt Spring Island in British Columbia, Canada. The new species has been found in the moss growing on rock and differs from the other species in the genus due to the presence of a median anterior mucrone\u0000 in the third band of the oral cavity armature (OCA) and by the presence of evident thickenings on the eggshell connecting the neighbouring processes. This finding highlights the importance of continuing to study tardigrade biodiversity, even in already explored areas, and how an integrative\u0000 approach is fundamental to achieving a reliable measure of biodiversity.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42646349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Bojarski, D. Medkova, A. Hollerova, Leszek Szała, B. Stonawski, M. Chmurska-Gąsowska
A haematological analysis is commonly used in studies concerning fish, e.g.to evaluate the influence of various environmental factors on the physiological state of a population. Furthermore, a manual haematology is still used for conducting fish blood analyses. The aim of the current study was to determine how long after the procedure of blood collection in common carp (Cyprinus carpio) the samples diluted with Hayem's solution would provide results for the red blood cell and white blood cell counts with no significant changes. The blood was taken from 15 healthy individuals and diluted with Hayem's solution immediately after the collection procedure. Next, an erythrocyte count and leukocyte count were performed. The counts were repeated after 1, 2, 3, 5, 9, 16 and 23 days. The results of the current study indicate that the erythrocyte count should be performed within a maximum of 16 days (or better within 9 days) after the sample preparation, while the leukocyte count should be conducted within 2 days.
{"title":"Common Carp (Cyprinus carpio) Blood Cells in Hayem's Solution – Stable or Not?","authors":"B. Bojarski, D. Medkova, A. Hollerova, Leszek Szała, B. Stonawski, M. Chmurska-Gąsowska","doi":"10.3409/fb_70-3.13","DOIUrl":"https://doi.org/10.3409/fb_70-3.13","url":null,"abstract":"A haematological analysis is commonly used in studies concerning fish, e.g.to evaluate the influence of various environmental factors on the physiological state of a population. Furthermore, a manual haematology is still used for conducting fish blood analyses. The aim of the current\u0000 study was to determine how long after the procedure of blood collection in common carp (Cyprinus carpio) the samples diluted with Hayem's solution would provide results for the red blood cell and white blood cell counts with no significant changes. The blood was taken from 15 healthy\u0000 individuals and diluted with Hayem's solution immediately after the collection procedure. Next, an erythrocyte count and leukocyte count were performed. The counts were repeated after 1, 2, 3, 5, 9, 16 and 23 days. The results of the current study indicate that the erythrocyte count should\u0000 be performed within a maximum of 16 days (or better within 9 days) after the sample preparation, while the leukocyte count should be conducted within 2 days.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47421343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cimigenoside, one of the main elements isolated from the aboveground plants of the Cimicifuga genus, has anti-tumour effects. However, the question of whether Cimigenoside had effects on lung cancer remains elusive. This study was aimed at investigating the effects of Cimigenoside on A549 lung cancer cells. The A549 cells were cultured in vitro, and an MTT assay was carried out to explore the cell proliferative abilities after a treatment with Cimigenoside. The wound healing assays and Transwell invasion assays were carried out to explore the effects of Cimigenoside on cell migration and invasion in the A549 cells. A cell apoptosis assay using flow cytometry was used to evaluate the effects on cell apoptosis in the A549 cells. The related protein expression levels were measured using a Western blot assay. It was hypothesised that Cimigenoside could restrain tumour cell proliferation, migration and invasion, while inducing cell apoptosis with apoptosis-related protein change levels in a time- and dose-dependent manner. The Western blot assay revealed that the Cimigenoside treated A549 cells had a reduced expression of p65, while having an increased expression of IκBα at the protein level. Taken together, this study demonstrated that Cimigenoside has the potential to repress cell proliferation, migration and invasion, while inducing apoptosis in A549 lung cancer cells through the NF-κB pathway. These results reveal that Cimigenoside may have a therapeutic potential for lung cancer treatment.
{"title":"Cimigenoside Affects Cell Viability, Apoptosis and Metastasis Of A549 Cells Via The NF-κB Pathway","authors":"Qinqin Wang, H. Yang, Wenjun Yang, Tingshu Jiang","doi":"10.3409/fb_70-2.10","DOIUrl":"https://doi.org/10.3409/fb_70-2.10","url":null,"abstract":"Cimigenoside, one of the main elements isolated from the aboveground plants of the Cimicifuga genus, has anti-tumour effects. However, the question of whether Cimigenoside had effects on lung cancer remains elusive. This study was aimed at investigating the effects of Cimigenoside\u0000 on A549 lung cancer cells. The A549 cells were cultured in vitro, and an MTT assay was carried out to explore the cell proliferative abilities after a treatment with Cimigenoside. The wound healing assays and Transwell invasion assays were carried out to explore the effects of Cimigenoside\u0000 on cell migration and invasion in the A549 cells. A cell apoptosis assay using flow cytometry was used to evaluate the effects on cell apoptosis in the A549 cells. The related protein expression levels were measured using a Western blot assay. It was hypothesised that Cimigenoside could restrain\u0000 tumour cell proliferation, migration and invasion, while inducing cell apoptosis with apoptosis-related protein change levels in a time- and dose-dependent manner. The Western blot assay revealed that the Cimigenoside treated A549 cells had a reduced expression of p65, while having an increased\u0000 expression of IκBα at the protein level. Taken together, this study demonstrated that Cimigenoside has the potential to repress cell proliferation, migration and invasion, while inducing apoptosis in A549 lung cancer cells through the NF-κB pathway. These results reveal that\u0000 Cimigenoside may have a therapeutic potential for lung cancer treatment.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41319487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anatolia has played an important role in the speciation of many species. Global climatic changes affect the distribution of many reptiles in different ways, including their range expansion or habitat loss. In this study, we focused on the genus Anatololacerta which inhabits Anatolia and some Greek islands. In total, 150 literature records and 20 items of observation data were analysed with the maximum entropy method (MaxEnt) for the last glacial maximum (LGM), as well as historical (1970-2000) and future (2081-2100; RCP 4.5) bioclimatic conditions. According to our model, the distribution ranges in the future conditions for A. danfordi, A. finikensis and A. ibrahimi were more extensive than for the historical conditions. However, A. anatolica and A. pelasgiana may experience a potential habitat loss in the future. Although physical barriers were obstacles in the LGM and historical records, these barriers may be overcome after the climatic changes taking place in the near future.
{"title":"Ecological Niche Modelling of the Genus Anatololacerta Under Past, Historical And Future Bioclimatic Conditions","authors":"E. Bozkurt","doi":"10.3409/fb_70-2.06","DOIUrl":"https://doi.org/10.3409/fb_70-2.06","url":null,"abstract":"Anatolia has played an important role in the speciation of many species. Global climatic changes affect the distribution of many reptiles in different ways, including their range expansion or habitat loss. In this study, we focused on the genus Anatololacerta which inhabits Anatolia\u0000 and some Greek islands. In total, 150 literature records and 20 items of observation data were analysed with the maximum entropy method (MaxEnt) for the last glacial maximum (LGM), as well as historical (1970-2000) and future (2081-2100; RCP 4.5) bioclimatic conditions. According to our model,\u0000 the distribution ranges in the future conditions for A. danfordi, A. finikensis and A. ibrahimi were more extensive than for the historical conditions. However, A. anatolica and A. pelasgiana may experience a potential habitat loss in the future. Although\u0000 physical barriers were obstacles in the LGM and historical records, these barriers may be overcome after the climatic changes taking place in the near future.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46431593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Buczyński, Katarzyna Walczyk, Agnieszka Tańczuk, E. Buczyńska, Paweł Bojar, Nikola Góral
The negative impact of storage reservoirs on the environment has been well documented, but it appears that under certain circumstances these reservoirs can also help to protect biodiversity. The distribution of adult dragonflies and damselflies was studied in relation to eight environmental variables, in an upland storage reservoir and its feeder rivers located in South-East Poland (East-Central Europe). A total of 25 species were recorded, including 22 in the reservoir. Submerged and floating plants, width of the shallow littoral zone and the water movement/current were found to be the key drivers of the species distribution (pCCA, NMDS). Five species (Ischnura elegans, Sympetrum sanguineum, Platycnemis pennipes, Calopteryx splendens and Erythromma viridulum) were responsible for over 70% of the dissimilarities between the riverine and reservoir sites (SIMPER). In addition, Ischnura elegans, Orthetrum albistylum and Calopteryx virgo were distinguished as the indicator species (IndVal analysis) for the upland river-reservoir hydrological system. Our results highlight some design features of reservoirs that may help to maintain the diversity of odonates, as well as many other groups of aquatic organisms, as the former are well-known indicators of general biodiversity.
{"title":"Structural and Physical Factors As Predictors of the Species Distribution And Diversity of Dragonflies and Damselflies (Odonata) in an Upland Storage Reservoir","authors":"P. Buczyński, Katarzyna Walczyk, Agnieszka Tańczuk, E. Buczyńska, Paweł Bojar, Nikola Góral","doi":"10.3409/fb_70-2.08","DOIUrl":"https://doi.org/10.3409/fb_70-2.08","url":null,"abstract":"The negative impact of storage reservoirs on the environment has been well documented, but it appears that under certain circumstances these reservoirs can also help to protect biodiversity. The distribution of adult dragonflies and damselflies was studied in relation to eight environmental\u0000 variables, in an upland storage reservoir and its feeder rivers located in South-East Poland (East-Central Europe). A total of 25 species were recorded, including 22 in the reservoir. Submerged and floating plants, width of the shallow littoral zone and the water movement/current were found\u0000 to be the key drivers of the species distribution (pCCA, NMDS). Five species (Ischnura elegans, Sympetrum sanguineum, Platycnemis pennipes, Calopteryx splendens and Erythromma viridulum) were responsible for over 70% of the dissimilarities between the riverine\u0000 and reservoir sites (SIMPER). In addition, Ischnura elegans, Orthetrum albistylum and Calopteryx virgo were distinguished as the indicator species (IndVal analysis) for the upland river-reservoir hydrological system. Our results highlight some design features of reservoirs that\u0000 may help to maintain the diversity of odonates, as well as many other groups of aquatic organisms, as the former are well-known indicators of general biodiversity.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69767418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patrycja Wójcicka, Bożena Wlizło-Skowronek, D. Żakowska, Alicja Mełgieś-Podkowiak, Paweł Rutyna, G. Raniak
In this work, ticks were collected from the military area of the West Pomeranian Voivodeship in Poland, and the most important pathogens were detected using the real-time PCR method. The ticks were collected in mid-spring (April and May) using the flagging technique. They were collected in five places: on the shoreline of Drawa River and Konotop Lake, as well as in forest and meadow areas near Karwice, Konotop and Oleszno Villages. During the work, only one species of ticks was caught – Ixodes ricinus. All the collected specimens were assessed in terms of their species, sex and developmental stage by zoological specialists. The largest number of specimens was caught in Karwice (35%, n = 666). The highest number of ticks in each test site were nymphs (57%, n = 1106), except at Konotop. The reaction of RT-PCR was tested in 111 pools of ticks. As a result of the PCR reactions (VIASURE Tick-Borne Diseases Real-Time PCR Detection Kit), Rickettsia spp. (n = 70) and Borrelia burgdorferi s. l. (n = 35) were determined to be the most frequently identified pathogens in the tick samples. Other pathogens such as Anaplasma phagocytophilum and Ehrlichia chaffeensis were detected in a smaller number of samples. The results in this paper confirm the epidemiological data collected and analysed prior to now.
{"title":"The Occurrence of Tick-Borne Pathogens in Samples Collected from the West Pomeranian Voivodeship","authors":"Patrycja Wójcicka, Bożena Wlizło-Skowronek, D. Żakowska, Alicja Mełgieś-Podkowiak, Paweł Rutyna, G. Raniak","doi":"10.3409/fb_70-2.09","DOIUrl":"https://doi.org/10.3409/fb_70-2.09","url":null,"abstract":"In this work, ticks were collected from the military area of the West Pomeranian Voivodeship in Poland, and the most important pathogens were detected using the real-time PCR method. The ticks were collected in mid-spring (April and May) using the flagging technique. They were collected\u0000 in five places: on the shoreline of Drawa River and Konotop Lake, as well as in forest and meadow areas near Karwice, Konotop and Oleszno Villages. During the work, only one species of ticks was caught – Ixodes ricinus. All the collected specimens were assessed in terms of their\u0000 species, sex and developmental stage by zoological specialists. The largest number of specimens was caught in Karwice (35%, n = 666). The highest number of ticks in each test site were nymphs (57%, n = 1106), except at Konotop. The reaction of RT-PCR was tested in 111 pools of ticks. As a\u0000 result of the PCR reactions (VIASURE Tick-Borne Diseases Real-Time PCR Detection Kit), Rickettsia spp. (n = 70) and Borrelia burgdorferi s. l. (n = 35) were determined to be the most frequently identified pathogens in the tick samples. Other pathogens such as Anaplasma phagocytophilum\u0000 and Ehrlichia chaffeensis were detected in a smaller number of samples. The results in this paper confirm the epidemiological data collected and analysed prior to now.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45385718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xinlu Wang, Xiaojie Zhang, T. Lu, L. Niu, Linjie Wang, S. Zhan, Jiazhong Guo, Jiaxue Cao, Li Li, Hongping Zhang
In this study, we aimed to identify genetic variants of the TLR5 gene and the promoter methylation level with a susceptibility to brucellosis in Chinese Saanen dairy goats (SN). We randomly collected 205 genetically unrelated animals from five Chinese goat breeds, including the Saanen dairy goat (SN, n = 67), Tibetan goat (TG, n = 35), Chuannan black goat (CN, n = 30), Meigu goat (MG, n = 37) and the Jianzhou big-ear goat (JZ, n = 36). A blood sample was collected from each individual and used for the detection of brucellosis, TLR5 polymorphism identification and the detection of promoter methylation. In total, twenty-four SNPs were identified in the five investigated breeds. The allele and genotype frequency of the four loci (g.435C>T, g.690G>T, g.978A>G and g.1832A>G) were correlated with a seroprevalence of brucellosis in the SN goats. An r2 linkage disequilibrium (LD) was detected in SN, CN, TG and MG, but not in the JZ goats. Ten methylation sites were detected on the first CpG island with DNA methylation levels ranging from 0.2% to 5.2%, and twenty-two methylation sites were found on the second methylation island with a 2.3% to 12.1% methylation. The methylation levels of the two CpG islands in the healthy SN were significantly higher than those recorded in the brucella -infected SN (p <0.05). Our findings demonstrated that genetic variations and the promoter methylation of TLR5 were associated with a brucellosis risk in SN goats. These results will be helpful for further studies of disease resistance in ruminants.
在这项研究中，我们旨在确定中国萨宁奶山羊(SN) TLR5基因的遗传变异和启动子甲基化水平与布鲁氏菌病的易感性。随机选取5个中国山羊品种205只，分别为萨宁奶山羊(SN, n = 67)、藏山羊(TG, n = 35)、川南黑山羊(CN, n = 30)、美姑山羊(MG, n = 37)和建州大耳山羊(JZ, n = 36)。从每个人身上采集血样，用于布鲁氏菌病检测、TLR5多态性鉴定和启动子甲基化检测。在5个被调查品种中共鉴定出24个snp。4个基因座(G . 435c >T、G . 690g >T、G . 978a >G和G . 1832a >G)的等位基因和基因型频率与SN山羊布鲁氏菌病血清患病率相关。SN、CN、TG和MG山羊均存在r2连锁不平衡(LD)，而JZ山羊不存在。在第一个CpG岛上检测到10个甲基化位点，DNA甲基化水平为0.2% ~ 5.2%;在第二个甲基化岛上检测到22个甲基化位点，DNA甲基化水平为2.3% ~ 12.1%。健康SN中两个CpG岛的甲基化水平显著高于感染SN (p <0.05)。我们的研究结果表明，遗传变异和TLR5启动子甲基化与SN山羊的布鲁氏菌病风险相关。这些结果将有助于反刍动物抗病能力的进一步研究。
{"title":"Nucleotide Variants in TheTLR5 Gene and Promoter Methylation with A Susceptibility to Brucellosis in Chinese Goats","authors":"Xinlu Wang, Xiaojie Zhang, T. Lu, L. Niu, Linjie Wang, S. Zhan, Jiazhong Guo, Jiaxue Cao, Li Li, Hongping Zhang","doi":"10.3409/fb_70-2.07","DOIUrl":"https://doi.org/10.3409/fb_70-2.07","url":null,"abstract":"In this study, we aimed to identify genetic variants of the TLR5 gene and the promoter methylation level with a susceptibility to brucellosis in Chinese Saanen dairy goats (SN). We randomly collected 205 genetically unrelated animals from five Chinese goat breeds, including the\u0000 Saanen dairy goat (SN, n = 67), Tibetan goat (TG, n = 35), Chuannan black goat (CN, n = 30), Meigu goat (MG, n = 37) and the Jianzhou big-ear goat (JZ, n = 36). A blood sample was collected from each individual and used for the detection of brucellosis, TLR5 polymorphism identification\u0000 and the detection of promoter methylation. In total, twenty-four SNPs were identified in the five investigated breeds. The allele and genotype frequency of the four loci (g.435C>T, g.690G>T, g.978A>G and g.1832A>G) were correlated with a seroprevalence of brucellosis in the SN\u0000 goats. An r2 linkage disequilibrium (LD) was detected in SN, CN, TG and MG, but not in the JZ goats. Ten methylation sites were detected on the first CpG island with DNA methylation levels ranging from 0.2% to 5.2%, and twenty-two methylation sites were found on the second methylation\u0000 island with a 2.3% to 12.1% methylation. The methylation levels of the two CpG islands in the healthy SN were significantly higher than those recorded in the brucella -infected SN (p <0.05). Our findings demonstrated that genetic variations and the promoter methylation of TLR5\u0000 were associated with a brucellosis risk in SN goats. These results will be helpful for further studies of disease resistance in ruminants.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43083196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Havvanur Yoldas Ilktac, G. Kiziltan, Asli Devrim Lanpir, M. Ozansoy, M. Gunal, S. O. Toğay, I. Keskin, E. M. Ozdemir, U. Kilic
We published the abovementioned article about probiotic mixture VSL#3 on October 6, 2021. Since the product was named VSL#3 in our purchasing process in early 2016, it is referred to as VSL#3 in our article. In addition, the product currently known as VSL#3 is not the same as De Simone Formulation. De Simone Formulation is now available as Visbiome® in the United States and Vivomixx® in Europe. The authors would like to apologise for any inconvenience caused
{"title":"Corrigendum to: Combined Therapy with Probiotic VSL#3 and Omega-3 Fatty Acids Attenuates Colonic Injury and Inflammation in Chronic DNBS-induced Colitis in Mice","authors":"Havvanur Yoldas Ilktac, G. Kiziltan, Asli Devrim Lanpir, M. Ozansoy, M. Gunal, S. O. Toğay, I. Keskin, E. M. Ozdemir, U. Kilic","doi":"10.3409/fb_70-1.05","DOIUrl":"https://doi.org/10.3409/fb_70-1.05","url":null,"abstract":"We published the abovementioned article about probiotic mixture VSL#3 on October 6, 2021. Since the product was named VSL#3 in our purchasing process in early 2016, it is referred to as VSL#3 in our article. In addition, the product currently known as VSL#3 is not the same as De Simone\u0000 Formulation. De Simone Formulation is now available as Visbiome® in the United States and Vivomixx® in Europe. The authors would like to apologise for any inconvenience caused","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43852970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Jakubiak, B. Bojarski, M. Bien, B. Stonawski, P. Oglęcki
Fish production can generate high amounts of wastewater containing compounds such as suspended solids, nitrogen and phosphorus. On the other hand, fish ponds provide a range of ecological functions including biocenotic, physiocenotic, hydrological and microclimatic functions as well as landscape shaping. The aim of this study was to determine the taxonomic composition of the bottom invertebrate fauna in selected watercourses on chosen carp fish farms and to assess the influence of fish farming on the taxonomic composition of the zoobenthos of the watercourses associated with such ponds. The research was conducted in four channels on two semi-intensive aquaculture fish farms located in Zaborze and Kraków (Poland). Sampling of the benthic fauna was carried out once a month between May and September 2018. The results showed that the mean monthly number of zoobenthos families in the inflow canal in Zaborze was higher than those in the outflow canal. Moreover, a decrease of the BMWP-PL index in the aforementioned farm may indicate a deterioration of the water quality resulting from its flow through the ponds. It can therefore be concluded that semi-intensive carp farming may affect the diversity of benthic invertebrate fauna in the watercourses connected to these ponds.
{"title":"Influence of Fish Ponds on the Benthic Invertebrate Composition in Hydrological Networks of Selected Fish Farms in Southern Poland","authors":"M. Jakubiak, B. Bojarski, M. Bien, B. Stonawski, P. Oglęcki","doi":"10.3409/fb_70-1.02","DOIUrl":"https://doi.org/10.3409/fb_70-1.02","url":null,"abstract":"Fish production can generate high amounts of wastewater containing compounds such as suspended solids, nitrogen and phosphorus. On the other hand, fish ponds provide a range of ecological functions including biocenotic, physiocenotic, hydrological and microclimatic functions as well\u0000 as landscape shaping. The aim of this study was to determine the taxonomic composition of the bottom invertebrate fauna in selected watercourses on chosen carp fish farms and to assess the influence of fish farming on the taxonomic composition of the zoobenthos of the watercourses associated\u0000 with such ponds. The research was conducted in four channels on two semi-intensive aquaculture fish farms located in Zaborze and Kraków (Poland). Sampling of the benthic fauna was carried out once a month between May and September 2018. The results showed that the mean monthly number\u0000 of zoobenthos families in the inflow canal in Zaborze was higher than those in the outflow canal. Moreover, a decrease of the BMWP-PL index in the aforementioned farm may indicate a deterioration of the water quality resulting from its flow through the ponds. It can therefore be concluded\u0000 that semi-intensive carp farming may affect the diversity of benthic invertebrate fauna in the watercourses connected to these ponds.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47638871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Greifová, T. Jambor, K. Tokárová, N. Knížatová, N. Lukáč
Many studies have revealed that oxidative stress is a primary factor in the pathogenesis of male reproductive system dysfunctions. The strong antioxidant and cytoprotective effects of resveratrol have previously been demonstrated, but its effect in the context of the male reproduction remains unconvincing. To observe the biological activity of resveratrol in protecting the male reproductive function, hydrogen peroxide-induced oxidative stress in Leydig cells was used as a cell model. The aim of the present study was to examine if resveratrol could induce changes in the gap junction intercellular communication (GJIC), nitric oxide production, total oxidant status (TOS) and total antioxidant capacity (TAC) in TM3 Leydig cells subjected to H2O2. The Leydig cells were exposed to a resveratrol treatment (5, 10, 20, 50 and 100 μM) in the presence or absence of H2O2 (300/600 μM) during a 24 h in vitro culture. The cell lysates to assess TOS and TAC, NO production were quantified in a culture medium using the Griess method, and the Scrape Loading/Dye Transfer (SL/DT) technique was used for the determination of GJIC in the exposed TM3 Leydig cells. Treatment with higher doses of resveratrol alone led to a significantly increased TOS (p<0.05 with 100 μM) and NO production (p<0.05 with 50 μM and 100 μM), but significantly reduced TAC (p<0.01 with 100 μM) and GJIC (p<0.05 with 100 μM), while the SL/DT evaluation in the cells exposed to resveratrol at concentrations 5 μM (p<0.05) and 10 μM (p<0.01) revealed a significant stimulation of GJIC. The most potent cytoprotective or stimulatory effect of resveratrol in the cells co-exposed to oxidative stress (300 μM H2O2) was observed at a concentration of 10 μM in the case of GJIC, which was manifested by a significant increase in the values (p<0.05) compared to the control group treated with H2O2 alone.
{"title":"In Vitro Effect of Resveratrol Supplementation on Oxidative Balance and Intercellular Communication of Leydig Cells Subjected to Induced Oxidative Stress","authors":"H. Greifová, T. Jambor, K. Tokárová, N. Knížatová, N. Lukáč","doi":"10.3409/fb_70-1.03","DOIUrl":"https://doi.org/10.3409/fb_70-1.03","url":null,"abstract":"Many studies have revealed that oxidative stress is a primary factor in the pathogenesis of male reproductive system dysfunctions. The strong antioxidant and cytoprotective effects of resveratrol have previously been demonstrated, but its effect in the context of the male reproduction\u0000 remains unconvincing. To observe the biological activity of resveratrol in protecting the male reproductive function, hydrogen peroxide-induced oxidative stress in Leydig cells was used as a cell model. The aim of the present study was to examine if resveratrol could induce changes in the\u0000 gap junction intercellular communication (GJIC), nitric oxide production, total oxidant status (TOS) and total antioxidant capacity (TAC) in TM3 Leydig cells subjected to H2O2. The Leydig cells were exposed to a resveratrol treatment (5, 10, 20, 50 and 100 μM) in the\u0000 presence or absence of H2O2 (300/600 μM) during a 24 h in vitro culture. The cell lysates to assess TOS and TAC, NO production were quantified in a culture medium using the Griess method, and the Scrape Loading/Dye Transfer (SL/DT) technique was used for the\u0000 determination of GJIC in the exposed TM3 Leydig cells. Treatment with higher doses of resveratrol alone led to a significantly increased TOS (p<0.05 with 100 μM) and NO production (p<0.05 with 50 μM and 100 μM), but significantly reduced TAC (p<0.01 with 100 μM) and GJIC\u0000 (p<0.05 with 100 μM), while the SL/DT evaluation in the cells exposed to resveratrol at concentrations 5 μM (p<0.05) and 10 μM (p<0.01) revealed a significant stimulation of GJIC. The most potent cytoprotective or stimulatory effect of resveratrol in the cells co-exposed\u0000 to oxidative stress (300 μM H2O2) was observed at a concentration of 10 μM in the case of GJIC, which was manifested by a significant increase in the values (p<0.05) compared to the control group treated with H2O2 alone.","PeriodicalId":50438,"journal":{"name":"Folia Biologica-Krakow","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47925160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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