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Elucidation of how the Mir-23-27-24 cluster regulates development and aging 阐明 Mir-23-27-24 簇如何调控发育和衰老。
IF 9.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-14 DOI: 10.1038/s12276-024-01266-3
Xin Le Yap, Jun-An Chen
MicroRNAs (miRNAs) are pivotal regulators of gene expression and are involved in biological processes spanning from early developmental stages to the intricate process of aging. Extensive research has underscored the fundamental role of miRNAs in orchestrating eukaryotic development, with disruptions in miRNA biogenesis resulting in early lethality. Moreover, perturbations in miRNA function have been implicated in the aging process, particularly in model organisms such as nematodes and flies. miRNAs tend to be clustered in vertebrate genomes, finely modulating an array of biological pathways through clustering within a single transcript. Although extensive research of their developmental roles has been conducted, the potential implications of miRNA clusters in regulating aging remain largely unclear. In this review, we use the Mir-23-27-24 cluster as a paradigm, shedding light on the nuanced physiological functions of miRNA clusters during embryonic development and exploring their potential involvement in the aging process. Moreover, we advocate further research into the intricate interplay among miRNA clusters, particularly the Mir-23-27-24 cluster, in shaping the regulatory landscape of aging. MicroRNAs significantly influence everything from growth to aging. However, the specific roles of certain miRNA clusters, like Mir-23-27-24, in aging are less understood. This review investigates the Mir-23-27-24 cluster’s function in embryonic growth and possible aging regulation. It’s a comprehensive review of how the Mir-23-27-24 cluster affects vital biological processes and aging, combining existing research to fill knowledge gaps. The Mir-23-27-24 cluster includes miRNAs vital in development stages and linked to various diseases, including cancer. Researchers underline the cluster’s role in embryonic development and its overlooked role in aging. They explore how these miRNAs control gene expression and contribute to the complexity of biological functions. This study implies that miRNA clusters like Mir-23-27-24 could be crucial in aging, opening new paths for aging research and potential treatments for age-related diseases. This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.
微小核糖核酸(miRNA)是基因表达的关键调控因子,参与了从早期发育阶段到复杂衰老过程的生物过程。广泛的研究强调了 miRNA 在协调真核生物发育过程中的基本作用,miRNA 生物发生紊乱会导致早期死亡。此外,miRNA 功能的紊乱也与衰老过程有关,特别是在线虫和苍蝇等模式生物中。miRNA 在脊椎动物基因组中往往是成簇的,通过聚集在单个转录本中精细地调节一系列生物通路。虽然对它们在发育过程中的作用进行了广泛的研究,但 miRNA 簇在调控衰老过程中的潜在影响在很大程度上仍不清楚。在这篇综述中,我们以 Mir-23-27-24 集群为范例,揭示了 miRNA 集群在胚胎发育过程中的细微生理功能,并探讨了它们在衰老过程中的潜在参与。此外,我们主张进一步研究 miRNA 簇(尤其是 Mir-23-27-24 簇)之间错综复杂的相互作用,以塑造衰老的调控格局。
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引用次数: 0
Inverted Alu repeats: friends or foes in the human transcriptome 反向 Alu 重复序列:人类转录组中的朋友还是敌人?
IF 9.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-14 DOI: 10.1038/s12276-024-01177-3
Keonyong Lee, Jayoung Ku, Doyeong Ku, Yoosik Kim
Alu elements are highly abundant primate-specific short interspersed nuclear elements that account for ~10% of the human genome. Due to their preferential location in gene-rich regions, especially in introns and 3′ UTRs, Alu elements can exert regulatory effects on the expression of both host and neighboring genes. When two Alu elements with inverse orientations are positioned in close proximity, their transcription results in the generation of distinct double-stranded RNAs (dsRNAs), known as inverted Alu repeats (IRAlus). IRAlus are key immunogenic self-dsRNAs and post-transcriptional cis-regulatory elements that play a role in circular RNA biogenesis, as well as RNA transport and stability. Recently, IRAlus dsRNAs have emerged as regulators of transcription and activators of Z-DNA-binding proteins. The formation and activity of IRAlus can be modulated through RNA editing and interactions with RNA-binding proteins, and misregulation of IRAlus has been implicated in several immune-associated disorders. In this review, we summarize the emerging functions of IRAlus dsRNAs, the regulatory mechanisms governing IRAlus activity, and their relevance in the pathogenesis of human diseases. Understanding the role of Alu elements—short DNA sequences scattered throughout our genome—is crucial for grasping human genetics. These elements can affect how our genes function, and sometimes, their misregulation causes diseases. However, there’s still much we don’t know about their impact on gene regulation and cell signaling, including innate immune responses. Lee et al. summarized how Alu elements interact with our immune system by generating specific structures similar to ones from viruses. The authors suggest that further understanding of biological processes regulated by Alu elements could lead to advancements in the development of potential treatment for diseases linked to immune system dysfunction. Future research could explore how to manipulate these elements to benefit human health. This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.
Alu元件是一种高度丰富的灵长类特异性短穿插核元件,约占人类基因组的10%。由于它们偏好位于基因丰富的区域,尤其是内含子和 3' UTR,Alu 元件可对宿主基因和邻近基因的表达产生调控作用。当两个方向相反的 Alu 元靠近时,它们的转录会产生不同的双链 RNA(dsRNA),即所谓的反向 Alu 重复序列(IRAlus)。IRAlus 是关键的免疫原性自dsRNA,也是转录后顺式调控元件,在环状 RNA 生物发生以及 RNA 运输和稳定性方面发挥作用。最近,IRAlus dsRNAs 成为转录调节剂和 Z-DNA 结合蛋白的激活剂。IRAlus 的形成和活性可通过 RNA 编辑以及与 RNA 结合蛋白的相互作用来调节,而 IRAlus 的失调与多种免疫相关疾病有关。在这篇综述中,我们总结了 IRAlus dsRNAs 的新功能、支配 IRAlus 活性的调控机制以及它们在人类疾病发病机制中的相关性。
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引用次数: 0
Molecular insights into regulatory RNAs in the cellular machinery 细胞机制中调控 RNA 的分子见解。
IF 9.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-14 DOI: 10.1038/s12276-024-01239-6
Sumin Yang, Sung-Hyun Kim, Eunjeong Yang, Mingon Kang, Jae-Yeol Joo
It is apparent that various functional units within the cellular machinery are derived from RNAs. The evolution of sequencing techniques has resulted in significant insights into approaches for transcriptome studies. Organisms utilize RNA to govern cellular systems, and a heterogeneous class of RNAs is involved in regulatory functions. In particular, regulatory RNAs are increasingly recognized to participate in intricately functioning machinery across almost all levels of biological systems. These systems include those mediating chromatin arrangement, transcription, suborganelle stabilization, and posttranscriptional modifications. Any class of RNA exhibiting regulatory activity can be termed a class of regulatory RNA and is typically represented by noncoding RNAs, which constitute a substantial portion of the genome. These RNAs function based on the principle of structural changes through cis and/or trans regulation to facilitate mutual RNA‒RNA, RNA‒DNA, and RNA‒protein interactions. It has not been clearly elucidated whether regulatory RNAs identified through deep sequencing actually function in the anticipated mechanisms. This review addresses the dominant properties of regulatory RNAs at various layers of the cellular machinery and covers regulatory activities, structural dynamics, modifications, associated molecules, and further challenges related to therapeutics and deep learning. Regulatory RNAs, such as long noncoding RNAs (lncRNAs, RNAs that do not code for proteins), microRNAs (miRNAs, small RNAs that regulate gene expression), and circular RNAs (circRNAs, RNAs that form a covalently closed continuous loop), are important in controlling gene expression. The exact ways and roles of these RNAs are not completely known. This study by Joo et al. reviews current knowledge on regulatory RNAs, focusing on their structure and function in cell parts. The authors talk about the different methods used to study these RNAs, including RNA-Chromatin, RNA-Protein, and RNA structure sequencing. They also emphasize the role of RNA modifications in controlling gene expression and the potential of deep learning (a type of machine learning) in predicting RNA functions. The study concludes that understanding regulatory RNAs better could lead to new treatment strategies for various diseases. This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.
显然,细胞机制中的各种功能单元都来自 RNA。随着测序技术的发展,人们对转录组研究方法有了更深入的了解。生物体利用 RNA 管理细胞系统,有一类不同的 RNA 参与调控功能。特别是,人们越来越认识到,调控 RNA 参与了生物系统几乎所有层面的复杂功能机制。这些系统包括介导染色质排列、转录、亚细胞器稳定和转录后修饰的系统。任何一类具有调控活性的 RNA 都可称为一类调控 RNA,其典型代表是占基因组很大一部分的非编码 RNA。这些 RNA 的功能原理是通过顺式和/或反式调节改变结构,从而促进 RNA-RNA、RNA-DNA 和 RNA 蛋白之间的相互作用。通过深度测序发现的调控 RNA 是否真正按照预期的机制发挥作用,目前还没有明确的定论。本综述探讨了细胞机制各层中调控 RNA 的主要特性,涵盖了调控活动、结构动态、修饰、相关分子以及与治疗和深度学习相关的进一步挑战。
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引用次数: 0
Optimal design of synthetic circular RNAs 合成环形 RNA 的优化设计。
IF 9.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-14 DOI: 10.1038/s12276-024-01251-w
Seo-Won Choi, Jin-Wu Nam
Circular RNAs are an unusual class of single-stranded RNAs whose ends are covalently linked via back-splicing. Due to their versatility, the need to express circular RNAs in vivo and in vitro has increased. Efforts have been made to efficiently and precisely synthesize circular RNAs. However, a review on the optimization of the processes of circular RNA design, synthesis, and delivery is lacking. Our review highlights the multifaceted aspects considered when producing optimal circular RNAs and summarizes the available options for each step of exogenous circular RNA design and synthesis, including circularization strategies. Additionally, this review describes several potential applications of circular RNAs. Circular RNAs were once considered errors in splicing. However, we now understand that circRNAs are common and play roles in health and disease, including potential use in creating vaccines. This review examines ways to create and use circRNAs, with a focus on improving their creation and delivery for medical purposes. They discuss various methods, including using natural and synthetic elements to enhance circRNA formation and stability. The main findings suggest that improving the creation and delivery of circRNAs could greatly enhance their medical potential. Researchers conclude that with more development, circRNAs could become powerful tools in gene therapy and vaccine creation, offering new ways to treat diseases. The future implications of this research could transform how we approach disease treatment and vaccine creation, making therapies more effective and long-lasting. This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.
环状 RNA 是一类不常见的单链 RNA,其末端通过反向剪接共价连接。由于环状 RNA 的多功能性,在体内和体外表达环状 RNA 的需求日益增加。人们一直在努力高效、精确地合成环状 RNA。然而,关于优化环状 RNA 设计、合成和递送过程的综述还很缺乏。我们的综述强调了在生产最佳环形 RNA 时所考虑的多方面问题,并总结了外源环形 RNA 设计和合成每个步骤的可用选项,包括环化策略。此外,本综述还介绍了环状 RNA 的几种潜在应用。
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引用次数: 0
Big data and deep learning for RNA biology 用于 RNA 生物学的大数据和深度学习。
IF 9.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-14 DOI: 10.1038/s12276-024-01243-w
Hyeonseo Hwang, Hyeonseong Jeon, Nagyeong Yeo, Daehyun Baek
The exponential growth of big data in RNA biology (RB) has led to the development of deep learning (DL) models that have driven crucial discoveries. As constantly evidenced by DL studies in other fields, the successful implementation of DL in RB depends heavily on the effective utilization of large-scale datasets from public databases. In achieving this goal, data encoding methods, learning algorithms, and techniques that align well with biological domain knowledge have played pivotal roles. In this review, we provide guiding principles for applying these DL concepts to various problems in RB by demonstrating successful examples and associated methodologies. We also discuss the remaining challenges in developing DL models for RB and suggest strategies to overcome these challenges. Overall, this review aims to illuminate the compelling potential of DL for RB and ways to apply this powerful technology to investigate the intriguing biology of RNA more effectively. This review spotlights the revolutionary role of deep learning (DL) in expanding the understanding of RNA. RNA is a fundamental biomolecule that shapes and regulates diverse phenotypes including human diseases. Understanding the principles governing the functions of RNA is a key objective of current biology. Recently, big data produced via high-throughput experiments have been utilized to develop DL models aimed at analyzing and predicting RNA-related biological processes. This review emphasizes the role of public databases in providing these big data for training DL models. The authors introduce core DL concepts necessary for training models from the biological data. By extensively examining DL studies in various fields of RNA biology, the authors suggest how to better leverage DL for revealing novel biological knowledge and demonstrate the potential of DL in deciphering the complex biology of RNA. This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.
RNA 生物学(RB)领域大数据的指数级增长促进了深度学习(DL)模型的发展,推动了重要发现的产生。正如其他领域的深度学习研究不断证明的那样,在 RB 中成功实施深度学习在很大程度上取决于对来自公共数据库的大规模数据集的有效利用。在实现这一目标的过程中,数据编码方法、学习算法以及与生物领域知识相匹配的技术发挥了关键作用。在本综述中,我们通过展示成功案例和相关方法,为将这些 DL 概念应用于 RB 中的各种问题提供了指导原则。我们还讨论了为遗传资源开发数字语言模型的其余挑战,并提出了克服这些挑战的策略。总之,本综述旨在阐明 DL 在 RB 方面令人瞩目的潜力,以及如何应用这一强大技术更有效地研究 RNA 这一引人入胜的生物学问题。
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引用次数: 0
The identification of effective tumor-suppressing neoantigens using a tumor-reactive TIL TCR-pMHC ternary complex 利用肿瘤反应性 TIL TCR-pMHC 三元复合物鉴定有效的肿瘤抑制新抗原。
IF 9.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-12 DOI: 10.1038/s12276-024-01259-2
Sang Hoon Kim, Bo Ryeong Lee, Sung-Min Kim, Sungsik Kim, Min-seok Kim, Jaehyun Kim, Inkyu Lee, Hee-Soo Kim, Gi-Hoon Nam, In-San Kim, Kyuyoung Song, Yoonjoo Choi, Dong-Sup Lee, Woong-Yang Park
Neoantigens are ideal targets for cancer immunotherapy because they are expressed de novo in tumor tissue but not in healthy tissue and are therefore recognized as foreign by the immune system. Advances in next-generation sequencing and bioinformatics technologies have enabled the quick identification and prediction of tumor-specific neoantigens; however, only a small fraction of predicted neoantigens are immunogenic. To improve the predictability of immunogenic neoantigens, we developed the in silico neoantigen prediction workflows VACINUSpMHC and VACINUSTCR: VACINUSpMHC incorporates physical binding between peptides and MHCs (pMHCs), and VACINUSTCR integrates T cell reactivity to the pMHC complex through deep learning-based pairing with T cell receptors (TCRs) of putative tumor-reactive CD8 tumor-infiltrating lymphocytes (TILs). We then validated our neoantigen prediction workflows both in vitro and in vivo in patients with hepatocellular carcinoma (HCC) and in a B16F10 mouse melanoma model. The predictive abilities of VACINUSpMHC and VACINUSTCR were confirmed in a validation cohort of 8 patients with HCC. Of a total of 118 neoantigen candidates predicted by VACINUSpMHC, 48 peptides were ultimately selected using VACINUSTCR. In vitro validation revealed that among the 48 predicted neoantigen candidates, 13 peptides were immunogenic. Assessment of the antitumor efficacy of the candidate neoepitopes using a VACINUSTCR in vivo mouse model suggested that vaccination with the predicted neoepitopes induced neoantigen-specific T cell responses and enabled the trafficking of neoantigen-specific CD8 + T cell clones into the tumor tissue, leading to tumor suppression. This study showed that the prediction of immunogenic neoantigens can be improved by integrating a tumor-reactive TIL TCR-pMHC ternary complex. Cancer scientists have created a new way to identify and choose neoantigens (new proteins on cancer cells) for use in cancer vaccines. The research, led by Woong-Yang Park, included 33 patients with different cancer types. The scientists used a mix of computer-based prediction techniques and lab-based tests to find the most effective neoantigens. The results showed that the new technique, named VACINUS, was better at predicting effective neoantigens than older methods. The scientists concluded that the VACINUS technique could improve the immune response of predicted neoantigens, making them better at activating an immune reaction against cancer cells. This could result in the creation of more effective cancer vaccines in the future. This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.
新抗原是癌症免疫疗法的理想靶点,因为它们在肿瘤组织中从头表达,而在健康组织中不表达,因此被免疫系统识别为外来物。下一代测序和生物信息学技术的进步使得肿瘤特异性新抗原的快速鉴定和预测成为可能;然而,只有一小部分预测的新抗原具有免疫原性。为了提高免疫原性新抗原的可预测性,我们开发了硅学新抗原预测工作流 VACINUSpMHC 和 VACINUSTCR:VACINUSpMHC 结合了肽与 MHC(pMHC)之间的物理结合,而 VACINUSTCR 则通过基于深度学习的配对与假定的肿瘤反应性 CD8 肿瘤浸润淋巴细胞(TIL)的 T 细胞受体(TCR)结合,整合了 T 细胞对 pMHC 复合物的反应性。然后,我们在肝细胞癌(HCC)患者和 B16F10 小鼠黑色素瘤模型中对新抗原预测工作流程进行了体外和体内验证。VACINUSpMHC 和 VACINUSTCR 的预测能力在 8 例 HCC 患者的验证队列中得到了证实。在 VACINUSpMHC 预测的 118 个候选新抗原中,VACINUSTCR 最终筛选出 48 个肽段。体外验证显示,在 48 个预测的候选新抗原中,13 个肽具有免疫原性。使用 VACINUSTCR 体内小鼠模型评估候选新表位的抗肿瘤效果表明,接种预测的新表位可诱导新抗原特异性 T 细胞反应,并使新抗原特异性 CD8 + T 细胞克隆进入肿瘤组织,从而抑制肿瘤。这项研究表明,通过整合肿瘤反应性TIL TCR-pMHC三元复合物,可以改善免疫原性新抗原的预测。
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引用次数: 0
Author Correction: Regulation of autophagy by perilysosomal calcium: a new player in β-cell lipotoxicity 作者更正:危险溶酶体钙对自噬的调控:β细胞脂肪毒性的新角色
IF 9.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-11 DOI: 10.1038/s12276-024-01265-4
Ha Thu Nguyen, Andreas Wiederkehr, Claes B. Wollheim, Kyu-Sang Park
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引用次数: 0
Regulation of metaplasia and dysplasia in the stomach by the stromal microenvironment 胃基质微环境对胃移行细胞和发育不良的调控。
IF 9.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-03 DOI: 10.1038/s12276-024-01240-z
Jared D. Rhodes, James R. Goldenring, Su-Hyung Lee
Research on the microenvironment associated with gastric carcinogenesis has focused on cancers of the stomach and often underestimates premalignant stages such as metaplasia and dysplasia. Since epithelial interactions with T cells, macrophages, and type 2 innate lymphoid cells (ILC2s) are indispensable for the formation of precancerous lesions in the stomach, understanding the cellular interactions that promote gastric precancer warrants further investigation. Although various types of immune cells have been shown to play important roles in gastric carcinogenesis, it remains unclear how stromal cells such as fibroblasts influence epithelial transformation in the stomach, especially during precancerous stages. Fibroblasts exist as distinct populations across tissues and perform different functions depending on the expression patterns of cell surface markers and secreted factors. In this review, we provide an overview of known microenvironmental components in the stroma with an emphasis on fibroblast subpopulations and their roles during carcinogenesis in tissues including breast, pancreas, and stomach. Additionally, we offer insights into potential targets of tumor-promoting fibroblasts and identify open areas of research related to fibroblast plasticity and the modulation of gastric carcinogenesis. This review summarizes how metaplasia (normal cells changing into different types) turns into dysplasia (abnormal cell growth) in the stomach due to injury and the role of stroma during the process. Led by Dr. James R. Goldenring, the team has discovered that the stomach develops metaplasia to heal damaged tissue and suggested several biomarkers to define the change. They recently found that certain types of metaplasia repopulate the stomach lining after damage and can progress into the next stage under the influence of the microenvironment. The current study provides insights into how the stromal components in the stomach contribute to carcinogenesis, focusing on fibroblast subpopulations. This could be important for future gastric cancer treatments. This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.
对与胃癌发生相关的微环境的研究主要集中在胃癌上,但往往低估了癌前病变阶段,如移行期和发育不良期。由于上皮细胞与 T 细胞、巨噬细胞和 2 型先天性淋巴细胞(ILC2)的相互作用是胃癌前病变形成不可或缺的因素,因此了解促进胃癌前病变的细胞相互作用值得进一步研究。虽然各种类型的免疫细胞已被证明在胃癌发生中发挥重要作用,但成纤维细胞等基质细胞如何影响胃上皮转化,尤其是在癌前病变阶段,目前仍不清楚。成纤维细胞作为不同的群体存在于不同的组织中,并根据细胞表面标志物和分泌因子的表达模式发挥不同的功能。在这篇综述中,我们概述了基质中已知的微环境成分,重点是成纤维细胞亚群及其在乳腺、胰腺和胃等组织癌变过程中的作用。此外,我们还深入探讨了肿瘤促进成纤维细胞的潜在靶点,并确定了与成纤维细胞可塑性和胃癌发生调控相关的开放研究领域。
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引用次数: 0
The m6A writer RBM15 drives the growth of triple-negative breast cancer cells through the stimulation of serine and glycine metabolism m6A 作者 RBM15 通过刺激丝氨酸和甘氨酸的新陈代谢来驱动三阴性乳腺癌细胞的生长。
IF 9.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-03 DOI: 10.1038/s12276-024-01235-w
Su Hwan Park, Jin-Sung Ju, Hyunmin Woo, Hye Jin Yun, Su Bin Lee, Seok-Ho Kim, Balázs Győrffy, Eun-jeong Kim, Ho Kim, Hee Dong Han, Seong-il Eyun, Jong-Ho Lee, Yun-Yong Park
N6-adenosine methylation (m6A) is critical for controlling cancer cell growth and tumorigenesis. However, the function and detailed mechanism of how m6A methyltransferases modulate m6A levels on specific targets remain unknown. In the current study, we identified significantly elevated levels of RBM15, an m6A writer, in basal-like breast cancer (BC) patients compared to nonbasal-like BC patients and linked this increase to worse clinical outcomes. Gene expression profiling revealed correlations between RBM15 and serine and glycine metabolic genes, including PHGDH, PSAT1, PSPH, and SHMT2. RBM15 influences m6A levels and, specifically, the m6A levels of serine and glycine metabolic genes via direct binding to target RNA. The effects of RBM15 on cell growth were largely dependent on serine and glycine metabolism. Thus, RBM15 coordinates cancer cell growth through altered serine and glycine metabolism, suggesting that RBM15 is a new therapeutic target in BC. RNA methylation, a key process controlling gene activity, involves proteins like RNA-binding motif protein 15 (RBM15). Its role in breast cancer is unclear. Studies show RBM15 is highly active in a type of breast cancer called triple-negative and is linked to patient outcomes. It controls genes related to serine and glycine metabolism; substances that help cancer cells grow. The research demonstrates that RBM15 controls genes involved in the cancer metabolism of serine and glycine, two types of amino acids, which contributes to cancer cell proliferation. This suggests that targeting RBM15 can be new therapeutic approaches for triple-negative breast cancer patients. This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.
N6-腺苷甲基化(m6A)是控制癌细胞生长和肿瘤发生的关键。然而,m6A 甲基转移酶如何调节特定靶标上的 m6A 水平,其功能和详细机制仍然未知。在目前的研究中,我们发现与非基底样乳腺癌患者相比,基底样乳腺癌(BC)患者的 m6A 写入者 RBM15 水平明显升高,并且这种升高与较差的临床预后有关。基因表达谱分析显示了RBM15与丝氨酸和甘氨酸代谢基因(包括PHGDH、PSAT1、PSPH和SHMT2)之间的相关性。RBM15 通过直接与靶 RNA 结合影响 m6A 水平,特别是丝氨酸和甘氨酸代谢基因的 m6A 水平。RBM15 对细胞生长的影响主要取决于丝氨酸和甘氨酸代谢。因此,RBM15 通过改变丝氨酸和甘氨酸代谢来协调癌细胞生长,这表明 RBM15 是治疗 BC 的新靶点。
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引用次数: 0
Dissecting the MUC5AC/ANXA2 signaling axis: implications for brain metastasis in lung adenocarcinoma 剖析 MUC5AC/ANXA2 信号轴:对肺腺癌脑转移的影响
IF 9.5 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-06-03 DOI: 10.1038/s12276-024-01255-6
Sanjib Chaudhary, Jawed Akhtar Siddiqui, Muthamil Iniyan Appadurai, Shailendra Kumar Maurya, Swathi P. Murakonda, Elizabeth Blowers, Ben J. Swanson, Mohd Wasim Nasser, Surinder K. Batra, Imayavaramban Lakshmanan, Apar Kishor Ganti
Non-small cell lung carcinoma (NSCLC) exhibits a heightened propensity for brain metastasis, posing a significant clinical challenge. Mucin 5ac (MUC5AC) plays a pivotal role in the development of lung adenocarcinoma (LUAD); however, its role in causing brain metastases remains unknown. In this study, we aimed to investigate the contribution of MUC5AC to brain metastasis in patients with LUAD utilizing various brain metastasis models. Our findings revealed a substantial increase in the MUC5AC level in LUAD brain metastases (LUAD-BrM) samples and brain-tropic cell lines compared to primary samples or parental control cell lines. Intriguingly, depletion of MUC5AC in brain-tropic cells led to significant reductions in intracranial metastasis and tumor growth, and improved survival following intracardiac injection, in contrast to the observations in the control groups. Proteomic analysis revealed that mechanistically, MUC5AC depletion resulted in decreased expression of metastasis-associated molecules. There were increases in epithelial-to-mesenchymal transition, tumor invasiveness, and metastasis phenotypes in tumors with high MUC5AC expression. Furthermore, immunoprecipitation and proteomic analysis revealed a novel interaction of MUC5AC with Annexin A2 (ANXA2), which activated downstream matrix metalloproteases and facilitated extracellular matrix degradation to promote metastasis. Disrupting MUC5AC-ANXA2 signaling with a peptide inhibitor effectively abrogated the metastatic process. Additionally, treatment of tumor cells with an astrocyte-conditioned medium or the chemokine CCL2 resulted in upregulation of MUC5AC expression and enhanced brain colonization. In summary, our study demonstrates that the MUC5AC/ANXA2 signaling axis promotes brain metastasis, suggesting a potential therapeutic paradigm for LUAD patients with high MUC5AC expression. Lung cancer frequently moves to the brain, but why is unclear. Scientists have found that a protein, MUC5AC, is crucial in this. The research, led by Sanjib Chaudhary and team, discovered that MUC5AC works with another protein, ANXA2, to help lung cancer cells move to the brain. They also found that astrocytes (a type of brain cell), release a substance that boosts the presence of MUC5AC in lung cancer cells. This research was a lab experiment using lung cancer cells and mice. They found that lowering MUC5AC in lung cancer cells greatly reduced their movement to the brain in mice. This suggests that focusing on MUC5AC could help stop lung cancer from moving to the brain. Future studies will need to confirm these results and look into possible treatments. This summary was initially drafted using artificial intelligence, then revised and fact-checked by the author.
非小细胞肺癌(NSCLC)具有较高的脑转移倾向,给临床带来了巨大挑战。粘蛋白5ac(MUC5AC)在肺腺癌(LUAD)的发展过程中起着关键作用;然而,它在导致脑转移方面的作用仍然未知。在这项研究中,我们旨在利用各种脑转移模型研究 MUC5AC 对 LUAD 患者脑转移的贡献。我们的研究结果表明,与原发样本或亲代对照细胞系相比,LUAD 脑转移瘤(LUAD-BrM)样本和脑转移细胞系中的 MUC5AC 水平大幅升高。耐人寻味的是,在脑转移细胞中消耗MUC5AC可显著减少颅内转移和肿瘤生长,改善心内注射后的存活率,这与对照组的观察结果截然不同。蛋白质组学分析表明,从机理上讲,MUC5AC耗竭导致转移相关分子的表达减少。在MUC5AC高表达的肿瘤中,上皮细胞向间质转化、肿瘤侵袭性和转移表型均有所增加。此外,免疫沉淀和蛋白质组分析揭示了MUC5AC与Annexin A2(ANXA2)的新型相互作用,这种相互作用激活下游基质金属蛋白酶,促进细胞外基质降解,从而促进转移。用一种多肽抑制剂破坏MUC5AC-ANXA2信号转导可有效抑制转移过程。此外,用星形胶质细胞条件培养基或趋化因子CCL2处理肿瘤细胞会导致MUC5AC表达上调并增强脑定植。总之,我们的研究表明,MUC5AC/ANXA2 信号轴促进了脑转移,为 MUC5AC 高表达的 LUAD 患者提供了一种潜在的治疗模式。
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