Pub Date : 2024-03-26DOI: 10.29413/abs.2024-9.1.13
I. L. Kulikova, K. A. Aleksandrova
Background. Accommodation disorders in children with hypermetropia is one of the main factors in emmetropization disorders and maintenance of image defocusing. The most severe changes in accommodation are observed in children with anisometropia and hyperopia.The aim of the work. To evaluate the changes in the accommodative function of the eye in children with hyperopia, amblyopia, who underwent refractive laser surgery (RLS), as well as in children with spectacle and contact lens correction in combination with pleoptic treatment.Methods and materials. Group 1 consisted of 30 children after RLS; group 2 consisted of 29 children who had spectacle correction; group 3 consisted of 26 children who had soft contact lens correction; all children received pleoptic treatment. Clinical examination included the analysis of objective reserves of relative accommodation (RRA) and objective accommodative response (OAR) with an open field autorefractometer, and the results of accommodation measurement.Results. In 1.5 years, statistically significant changes were observed in the coefficient of accommodation response (CAR) of the amblyopic eye between the groups 1 and 2 – 0.12 ± 0.02 and 0.00 ± 0.1 relative units, respectively (p = 0.01). Similar statistically significant changes were obtained in OAR and objective RRA of the amblyopic eye. OAR in the group 1 was –2.1 ± 0.67 dpt, in the group 2 – –1.38 ± 0.19 dpt (p = 0.01). At the end of the observation, the OAR in the group 1 was –2.1 ± 0.67 dpt, the objective RRA – –2.1 ± 0.67 dpt; in the group 2 the OAR was –1.38 ± 0.19 dpt (p = 0.01), the objective RRA – –1.38 ± 0.19 dpt (p = 0.01). There were no statistically significant changes in these parameters of the amblyopic eye between the groups 1 and 3.Conclusion. There was an improvement of accommodation disorders in children with anisometropia, hyperopia and amblyopia after refractive laser surgery and in children with contact lens correction. Due to a decrease in the refractive indices of the amblyopic eye, the values of CAR, OAR and objective RRA after refractive laser surgery tended to be closer to the normal values.
{"title":"Changes in accommodation disorders in children with anisometropic amblyopia and hypermetropia","authors":"I. L. Kulikova, K. A. Aleksandrova","doi":"10.29413/abs.2024-9.1.13","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.13","url":null,"abstract":"Background. Accommodation disorders in children with hypermetropia is one of the main factors in emmetropization disorders and maintenance of image defocusing. The most severe changes in accommodation are observed in children with anisometropia and hyperopia.The aim of the work. To evaluate the changes in the accommodative function of the eye in children with hyperopia, amblyopia, who underwent refractive laser surgery (RLS), as well as in children with spectacle and contact lens correction in combination with pleoptic treatment.Methods and materials. Group 1 consisted of 30 children after RLS; group 2 consisted of 29 children who had spectacle correction; group 3 consisted of 26 children who had soft contact lens correction; all children received pleoptic treatment. Clinical examination included the analysis of objective reserves of relative accommodation (RRA) and objective accommodative response (OAR) with an open field autorefractometer, and the results of accommodation measurement.Results. In 1.5 years, statistically significant changes were observed in the coefficient of accommodation response (CAR) of the amblyopic eye between the groups 1 and 2 – 0.12 ± 0.02 and 0.00 ± 0.1 relative units, respectively (p = 0.01). Similar statistically significant changes were obtained in OAR and objective RRA of the amblyopic eye. OAR in the group 1 was –2.1 ± 0.67 dpt, in the group 2 – –1.38 ± 0.19 dpt (p = 0.01). At the end of the observation, the OAR in the group 1 was –2.1 ± 0.67 dpt, the objective RRA – –2.1 ± 0.67 dpt; in the group 2 the OAR was –1.38 ± 0.19 dpt (p = 0.01), the objective RRA – –1.38 ± 0.19 dpt (p = 0.01). There were no statistically significant changes in these parameters of the amblyopic eye between the groups 1 and 3.Conclusion. There was an improvement of accommodation disorders in children with anisometropia, hyperopia and amblyopia after refractive laser surgery and in children with contact lens correction. Due to a decrease in the refractive indices of the amblyopic eye, the values of CAR, OAR and objective RRA after refractive laser surgery tended to be closer to the normal values.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"112 30","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140380371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. G. Gorokhova, N. Mikhailova, A. G. Zhukova, A. S. Kazitskaya
Background. Indole and its derivatives are widely used in all areas of pharmaceutical production. The toxicometry of indole compounds has been sufficiently studied. At the same time, there is still no information on the toxic effect on individual organs and systems during long-term intake of most compounds.The aim. To carry out an experimental study of the toxic effect of indole and its derivative 1-benzylindole on the functional state of the heart and blood values.Materials and methods. The work was carried out on 46 white rats, divided into groups: control group (n = 22); animals receiving indole once a day for 1 month (n = 12); animals receiving 1-benzylindole once a day for 1 month (n = 12). The substances were administered intragastrically 5 days a week. The condition of the animals was assessed by integral parameters, peripheral blood parameters and biochemical serum tests, and morphological data.Results. Administration of indole and 1-benzylindole caused an increase in the electrical activity of the atria, a decrease in the duration of the QRS complex, and a statistically significant decrease in blood pressure and body temperature compared to the control group. The intake of indole and 1-benzylindole decreased the number of red blood cells and hemoglobin, increased the activity of aspartate aminotransferase and alanine aminotransferase, and increased the concentrations of urea, total cholesterol and triglycerides in the blood. Against the background of long-term exposure to indole and 1-benzylindole, dystrophic disorders, hypertrophic and atrophic changes in individual fibers with a pronounced congestion of the microcirculatory vessels were revealed in the heart of rats.Conclusion. Indole and 1-benzylindole in case of long-term intake lead to functional disorders of the cardiovascular system, which cause the development of arterial hypertension, coronary heart disease, and atherosclerotic vascular lesions. Preventive measures in industries with possible contact with indole and its derivatives should include regular medical examinations of workers with mandatory monitoring of electrocardiography and advanced indicators of general and biochemical blood tests.
{"title":"Studies of the effect of indole and its derivative 1-benzylindole on the functional state of the heart and blood values of laboratory rats","authors":"L. G. Gorokhova, N. Mikhailova, A. G. Zhukova, A. S. Kazitskaya","doi":"10.29413/abs.2024-9.1.7","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.7","url":null,"abstract":"Background. Indole and its derivatives are widely used in all areas of pharmaceutical production. The toxicometry of indole compounds has been sufficiently studied. At the same time, there is still no information on the toxic effect on individual organs and systems during long-term intake of most compounds.The aim. To carry out an experimental study of the toxic effect of indole and its derivative 1-benzylindole on the functional state of the heart and blood values.Materials and methods. The work was carried out on 46 white rats, divided into groups: control group (n = 22); animals receiving indole once a day for 1 month (n = 12); animals receiving 1-benzylindole once a day for 1 month (n = 12). The substances were administered intragastrically 5 days a week. The condition of the animals was assessed by integral parameters, peripheral blood parameters and biochemical serum tests, and morphological data.Results. Administration of indole and 1-benzylindole caused an increase in the electrical activity of the atria, a decrease in the duration of the QRS complex, and a statistically significant decrease in blood pressure and body temperature compared to the control group. The intake of indole and 1-benzylindole decreased the number of red blood cells and hemoglobin, increased the activity of aspartate aminotransferase and alanine aminotransferase, and increased the concentrations of urea, total cholesterol and triglycerides in the blood. Against the background of long-term exposure to indole and 1-benzylindole, dystrophic disorders, hypertrophic and atrophic changes in individual fibers with a pronounced congestion of the microcirculatory vessels were revealed in the heart of rats.Conclusion. Indole and 1-benzylindole in case of long-term intake lead to functional disorders of the cardiovascular system, which cause the development of arterial hypertension, coronary heart disease, and atherosclerotic vascular lesions. Preventive measures in industries with possible contact with indole and its derivatives should include regular medical examinations of workers with mandatory monitoring of electrocardiography and advanced indicators of general and biochemical blood tests.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"119 31","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lysophosphatidic acid (LPA) is a biologically active lipid mediator that regulates a number ofsignaling pathways involved in the pathogenesis of bronchial asthma. Attention to studying the relationship of LPA with LPA receptors (LPARs) and ion channels with transient receptor potential (TRP) is caused by their role in the initiation and development of bronchial obstruction, which suggests the development of new effective strategies for the treatment of bronchial asthma through blocking LPA synthesis and/or regulation of the activity of the ligand-receptor relationship.The aim of the review. To summarize ideas on the role of lysophosphatidic acid and its receptors in the pathogenesis of bronchial asthma based on the analysis of articles published in English in 2020–2023 from the PubMed database.Conclusion. The review summarizes recent literature data on the chemical structure, biosynthetic pathways and LPA receptors. It presents the information on the role of LPA, LPARs andTRP channels inthepathogenesis of bronchial asthma; summarizes the bronchial asthma therapeutic strategies targeting LPA, LPARs, andTRP channels. The review highlights not only a new perspective on understanding the mechanisms of initiation of asthmatic reactions, but also possible ways to manage them at the stage of correction of their development.
{"title":"Lysophosphatidic acid and itsreceptors: Role in bronchial asthma pathogenesis","authors":"O. Kytikova, T. Novgorodtseva, Y. K. Denisenko","doi":"10.29413/abs.2024-9.1.2","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.2","url":null,"abstract":"Lysophosphatidic acid (LPA) is a biologically active lipid mediator that regulates a number ofsignaling pathways involved in the pathogenesis of bronchial asthma. Attention to studying the relationship of LPA with LPA receptors (LPARs) and ion channels with transient receptor potential (TRP) is caused by their role in the initiation and development of bronchial obstruction, which suggests the development of new effective strategies for the treatment of bronchial asthma through blocking LPA synthesis and/or regulation of the activity of the ligand-receptor relationship.The aim of the review. To summarize ideas on the role of lysophosphatidic acid and its receptors in the pathogenesis of bronchial asthma based on the analysis of articles published in English in 2020–2023 from the PubMed database.Conclusion. The review summarizes recent literature data on the chemical structure, biosynthetic pathways and LPA receptors. It presents the information on the role of LPA, LPARs andTRP channels inthepathogenesis of bronchial asthma; summarizes the bronchial asthma therapeutic strategies targeting LPA, LPARs, andTRP channels. The review highlights not only a new perspective on understanding the mechanisms of initiation of asthmatic reactions, but also possible ways to manage them at the stage of correction of their development.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"113 45","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. A. Shcherbak, A. S. Medvedeva, T. Aksenova, N. M. Shcherbak, K. O. Aksenov
The role of arginine in the development of primary arterial hypertension continues to be clarified up to the present moment. During natural metabolic processes in cells, methylated forms of arginine are produced – symmetric (SDMA) and asymmetric (ADMA) dimethylarginine. ADMA is a nitric oxide synthase inhibitor and is now considered a well-established marker for endothelial dysfunction. SDMA is not a nitric oxide synthase inhibitor, but may indirectly reduce nitric oxide production through competitive interaction with cellular L-arginine.Currently, arginine preparations are practically not used for the treatment of primary arterial hypertension. This was the rationale for the given scientific review. The article summarizes the information available in the literature (2018–2022) on the pathogenetic mechanisms of the relationship between arginine and the development of impaired vascular tone. We used PubMed and RSCI databases for our review. Using keywords, 1784 publications were found over the past 5 years. The final selection criteria were time frame and matching keywords. The review provides data on the increased ADMA concentrations in experimental hypertensive animals and individuals with essential hypertension. The role of arginine metabolites in the genesis of endothelial dysfunction and arterial hypertension and the prospects for the therapeutic use of this compound are discussed.
{"title":"The role of L-arginine in the pathogenesis of essential arterial hypertension","authors":"V. A. Shcherbak, A. S. Medvedeva, T. Aksenova, N. M. Shcherbak, K. O. Aksenov","doi":"10.29413/abs.2024-9.1.4","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.4","url":null,"abstract":"The role of arginine in the development of primary arterial hypertension continues to be clarified up to the present moment. During natural metabolic processes in cells, methylated forms of arginine are produced – symmetric (SDMA) and asymmetric (ADMA) dimethylarginine. ADMA is a nitric oxide synthase inhibitor and is now considered a well-established marker for endothelial dysfunction. SDMA is not a nitric oxide synthase inhibitor, but may indirectly reduce nitric oxide production through competitive interaction with cellular L-arginine.Currently, arginine preparations are practically not used for the treatment of primary arterial hypertension. This was the rationale for the given scientific review. The article summarizes the information available in the literature (2018–2022) on the pathogenetic mechanisms of the relationship between arginine and the development of impaired vascular tone. We used PubMed and RSCI databases for our review. Using keywords, 1784 publications were found over the past 5 years. The final selection criteria were time frame and matching keywords. The review provides data on the increased ADMA concentrations in experimental hypertensive animals and individuals with essential hypertension. The role of arginine metabolites in the genesis of endothelial dysfunction and arterial hypertension and the prospects for the therapeutic use of this compound are discussed.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":" 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Kologrivova, O. Kharitonova, A. Dmitriukov, E. Kravchenko, O. Koshelskaya, T. Suslova
Background. Increase ofthe epicardial adipose tissue (EAT) thickness isassociated with development of inflammation and cardiovascular complications, however, there is no data on the relationship between EAT thickening and the number of immunosuppressive regulatory T lymphocytes.The aim. To study the number of circulating T regulatory lymphocytes and nuclear translocation ofthe FoxP3 transcription factor in patients with stable coronary heart disease (CHD) depending on the epicardial adipose tissue thickness.Materials and methods. We examined 30 patients with chronic stable CHD. The EAT thickness was measured by echocardiography. Patients were divided into groups depending on the presence and absence of EAT thickening above 5 mm (groups 1 and 2, respectively). Imaging flow cytometry was used to determine the number of T regulatory lymphocytes and the level of FoxP3 nuclear translocation. The concentration of cytokines and high sensitivity C-reactive protein (hsCRP) was determined using enzyme-linked immunosorbent assay in blood serum.Results. Anthropometric indicators of obesity and the severity of atherosclerosis were comparable between groups. In group 2, there was an increase in low-density lipoprotein cholesterol concentration (p = 0.043), ratio of low-density lipoprotein cholesterol tohigh-density lipoprotein cholesterol (p = 0.017) and the concentration ofhsCRP (p = 0.044) andIL-1β (p = 0.005), adecrease in the number and relative count of Tregulatory lymphocytes (p = 0.020 andp = 0.026, respectively), aswellas thenumber of cells withFoxP3 nuclear translocation (p = 0.018) compared togroup1. According tomultiple logistic regression, the concentration ofhsCRP, IL-1β and T regulatory lymphocytes relative count in total were the predictors of EAT thickening (accuracy 80 %; sensitivity 75 %; specificity 84,6 %; AUC = 0.89).Conclusions. Thickening of epicardial adipose tissue inpatients withcoronary heart disease is associated with a decrease in the number of T regulatory lymphocytes andFoxP3 nuclear translocation inthem in presence of comparable anthropometric parameters of obesity and the severity of coronary atherosclerosis.
背景。心外膜脂肪组织（EAT）厚度的增加与炎症和心血管并发症的发生有关，但目前还没有关于心外膜脂肪组织增厚与免疫抑制调节性T淋巴细胞数量之间关系的数据。研究稳定型冠心病（CHD）患者循环 T 调节性淋巴细胞的数量和 FoxP3 转录因子的核转位与心外膜脂肪组织厚度的关系。我们对 30 名慢性稳定型冠心病患者进行了研究。超声心动图测量了心外膜脂肪组织的厚度。根据心外膜脂肪组织增厚是否超过 5 毫米将患者分为几组（分别为第 1 组和第 2 组）。使用成像流式细胞术测定 T 调节淋巴细胞的数量和 FoxP3 核易位水平。用酶联免疫吸附法测定血清中细胞因子和高敏 C 反应蛋白（hsCRP）的浓度。各组肥胖的人体测量指标和动脉粥样硬化的严重程度相当。第 2 组的低密度脂蛋白胆固醇浓度（p = 0.043）、低密度脂蛋白胆固醇与高密度脂蛋白胆固醇的比值（p = 0.017）和 hsCRP 浓度（p = 0.044) 和 IL-1β (p = 0.005)，与第一组相比，T 调节淋巴细胞的数量和相对数量增加（分别为 p = 0.020 和 p = 0.026），以及出现 FoxP3 核易位的细胞数量增加（p = 0.018）。根据多重逻辑回归，hsCRP 浓度、IL-1β 和 T 调节淋巴细胞相对总数是 EAT 增厚的预测因子（准确率 80%；灵敏度 75%；特异性 84.6%；AUC = 0.89）。结论：冠心病患者心外膜脂肪组织的增厚与 T 调节淋巴细胞数量的减少和其中的 FoxP3 核易位有关，而肥胖的人体测量参数和冠状动脉粥样硬化的严重程度具有可比性。
{"title":"Association of FoxP3+ T regulatory lymphocytes with epicardial adipose tissue thickness in patients with coronary heart disease","authors":"I. Kologrivova, O. Kharitonova, A. Dmitriukov, E. Kravchenko, O. Koshelskaya, T. Suslova","doi":"10.29413/abs.2024-9.1.5","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.5","url":null,"abstract":"Background. Increase ofthe epicardial adipose tissue (EAT) thickness isassociated with development of inflammation and cardiovascular complications, however, there is no data on the relationship between EAT thickening and the number of immunosuppressive regulatory T lymphocytes.The aim. To study the number of circulating T regulatory lymphocytes and nuclear translocation ofthe FoxP3 transcription factor in patients with stable coronary heart disease (CHD) depending on the epicardial adipose tissue thickness.Materials and methods. We examined 30 patients with chronic stable CHD. The EAT thickness was measured by echocardiography. Patients were divided into groups depending on the presence and absence of EAT thickening above 5 mm (groups 1 and 2, respectively). Imaging flow cytometry was used to determine the number of T regulatory lymphocytes and the level of FoxP3 nuclear translocation. The concentration of cytokines and high sensitivity C-reactive protein (hsCRP) was determined using enzyme-linked immunosorbent assay in blood serum.Results. Anthropometric indicators of obesity and the severity of atherosclerosis were comparable between groups. In group 2, there was an increase in low-density lipoprotein cholesterol concentration (p = 0.043), ratio of low-density lipoprotein cholesterol tohigh-density lipoprotein cholesterol (p = 0.017) and the concentration ofhsCRP (p = 0.044) andIL-1β (p = 0.005), adecrease in the number and relative count of Tregulatory lymphocytes (p = 0.020 andp = 0.026, respectively), aswellas thenumber of cells withFoxP3 nuclear translocation (p = 0.018) compared togroup1. According tomultiple logistic regression, the concentration ofhsCRP, IL-1β and T regulatory lymphocytes relative count in total were the predictors of EAT thickening (accuracy 80 %; sensitivity 75 %; specificity 84,6 %; AUC = 0.89).Conclusions. Thickening of epicardial adipose tissue inpatients withcoronary heart disease is associated with a decrease in the number of T regulatory lymphocytes andFoxP3 nuclear translocation inthem in presence of comparable anthropometric parameters of obesity and the severity of coronary atherosclerosis.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":" 72","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background. The increasingly complex structure of modern society and systemic changes associated with the processes of digitalization and mediatization are raising the issue of preserving the health of the younger generation, which is the most receptive and vulnerable group to external impact.The aim of the study. Based on survey data, to carry out an analysis of social factors influencing the development of eating disorders in girls.Materials and methods. The method of collecting information was an online survey of girls aged 18 to 25 years old, conducted in Yekaterinburg in the spring of 2023 (n = 205). Using in-depth interviews, 8 girls were interviewed to clarify the social factors in the formation of eating disorders.Results. In girls’ assessments, distorted body image, fear of weight gain, and orthorexia are the signs of eating disorders. Social factors influencing the formation of an ideal body image and eating disorders are social media, circle of contacts, as well as the external environment, which causes psychological and physical stress. Despite the negative perception of the content of communities promoting anorexic bodies, girls are well aware of the published materials. The main tool for achieving the standard model of a girl’s physicality is dietary practices, food restrictions and calorie counting, despite the recognition of the negative consequences of their use.Conclusion. Eating behavior for girls is one of the leading tools for achieving an ideal body, ideas about which are formed today by social media, as well as the immediate social environment, including the opposite sex. Communities on social networks can serve as a source for development of deviant eating attitudes, on the one hand, and a real driving force in providing assistance and support to young people with signs of eating disorders, on the other hand. We believe that online consultations with specialists and the development of a parental culture of health protection can have a significant impact on the development of harmonious, health-protecting eating habits.
{"title":"Social factors in the formation of eating disorders: Experience of sociological research","authors":"N. L. Antonova, D. V. Nagnoenko","doi":"10.29413/abs.2024-9.1.3","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.3","url":null,"abstract":"Background. The increasingly complex structure of modern society and systemic changes associated with the processes of digitalization and mediatization are raising the issue of preserving the health of the younger generation, which is the most receptive and vulnerable group to external impact.The aim of the study. Based on survey data, to carry out an analysis of social factors influencing the development of eating disorders in girls.Materials and methods. The method of collecting information was an online survey of girls aged 18 to 25 years old, conducted in Yekaterinburg in the spring of 2023 (n = 205). Using in-depth interviews, 8 girls were interviewed to clarify the social factors in the formation of eating disorders.Results. In girls’ assessments, distorted body image, fear of weight gain, and orthorexia are the signs of eating disorders. Social factors influencing the formation of an ideal body image and eating disorders are social media, circle of contacts, as well as the external environment, which causes psychological and physical stress. Despite the negative perception of the content of communities promoting anorexic bodies, girls are well aware of the published materials. The main tool for achieving the standard model of a girl’s physicality is dietary practices, food restrictions and calorie counting, despite the recognition of the negative consequences of their use.Conclusion. Eating behavior for girls is one of the leading tools for achieving an ideal body, ideas about which are formed today by social media, as well as the immediate social environment, including the opposite sex. Communities on social networks can serve as a source for development of deviant eating attitudes, on the one hand, and a real driving force in providing assistance and support to young people with signs of eating disorders, on the other hand. We believe that online consultations with specialists and the development of a parental culture of health protection can have a significant impact on the development of harmonious, health-protecting eating habits.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":" 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Belkova, E. Klimenko, U. Nemchenko, E. Grigorova, K. O. Sitnikova, R. E. Zugeeva, N. E. Smurova, N. Chemezova, E. Savilov
Klebsiella pneumoniae (Kp) species complex is a genetically and ecologically diverse group of bacteria that causes a wide range of infections in humans and animals.The aim. To carry out biological characterization and genotyping based on the study of different loci of Klebsiella pneumoniae clinical isolates.Materials and methods. The object of the study was three Klebsiella pneumoniae clinical isolates from different biotopes of patients from a regional children’s multidisciplinary hospital. We used a complex of bacteriological, molecular genetic and bioinformatic methods. Genotyping of the isolates was carried out using the Pasteur Institute service for strains of the K. pneumoniae species complex.Results. All strains were sensitive to antimicrobial drugs from carbapenem (imipenem, meropenem) and tetracycline groups (tigecycline), and demonstrated high sensitivity to the Klebsiella polyvalent bacteriophage. The antibiotic resistance of the Kp ODKB-16 and ODKB-81 isolates to seven and eight antimicrobial drugs, respectively, was registeredBased on the results of multi-locus sequence typing, all strains were assigned to Kp1 phylogroup, K2 type and differed in sequence type, scgMLST629 profile and KL type. Kp ODKB-16 strain was identified as ST-65, scgST-11107, KL2; ODKB-07 strain – as ST-219, scgST-6401, KL125KL114; ODKB-81 strain – as ST-86, scgST-2800, KL2KL30. The virulence gene clusters AbST, CbST, YbST, SmST, and RmST have been characterized only in the genome of the Kp ODKB-16 isolate, allowing it to be characterized as highly virulent with multidrug resistance (MDR). Additionally, genes responsible for the synthesis of types 1 and 3 fimbrial adhesins were registered in all strains, and ter operon loci were identified only in Kp ODKB-16. Resistome analysis showed that all strains had 2b genotype. Plasmids were found in the genomes of Kp ODKB-81 (IncI2) and ODKB-16 (IncFIA + IncFIB + IncHI1B).Conclusion. We used a comprehensive framework for genomic taxonomy of clinical isolates, which can contribute to the unification of global and regional peculiarities of the developing and microevolution of bacterial pathogens.
{"title":"Biological properties and genetic structure of clinic isolates of Klebsiella pneumoniae species","authors":"N. Belkova, E. Klimenko, U. Nemchenko, E. Grigorova, K. O. Sitnikova, R. E. Zugeeva, N. E. Smurova, N. Chemezova, E. Savilov","doi":"10.29413/abs.2024-9.1.6","DOIUrl":"https://doi.org/10.29413/abs.2024-9.1.6","url":null,"abstract":"Klebsiella pneumoniae (Kp) species complex is a genetically and ecologically diverse group of bacteria that causes a wide range of infections in humans and animals.The aim. To carry out biological characterization and genotyping based on the study of different loci of Klebsiella pneumoniae clinical isolates.Materials and methods. The object of the study was three Klebsiella pneumoniae clinical isolates from different biotopes of patients from a regional children’s multidisciplinary hospital. We used a complex of bacteriological, molecular genetic and bioinformatic methods. Genotyping of the isolates was carried out using the Pasteur Institute service for strains of the K. pneumoniae species complex.Results. All strains were sensitive to antimicrobial drugs from carbapenem (imipenem, meropenem) and tetracycline groups (tigecycline), and demonstrated high sensitivity to the Klebsiella polyvalent bacteriophage. The antibiotic resistance of the Kp ODKB-16 and ODKB-81 isolates to seven and eight antimicrobial drugs, respectively, was registeredBased on the results of multi-locus sequence typing, all strains were assigned to Kp1 phylogroup, K2 type and differed in sequence type, scgMLST629 profile and KL type. Kp ODKB-16 strain was identified as ST-65, scgST-11107, KL2; ODKB-07 strain – as ST-219, scgST-6401, KL125KL114; ODKB-81 strain – as ST-86, scgST-2800, KL2KL30. The virulence gene clusters AbST, CbST, YbST, SmST, and RmST have been characterized only in the genome of the Kp ODKB-16 isolate, allowing it to be characterized as highly virulent with multidrug resistance (MDR). Additionally, genes responsible for the synthesis of types 1 and 3 fimbrial adhesins were registered in all strains, and ter operon loci were identified only in Kp ODKB-16. Resistome analysis showed that all strains had 2b genotype. Plasmids were found in the genomes of Kp ODKB-81 (IncI2) and ODKB-16 (IncFIA + IncFIB + IncHI1B).Conclusion. We used a comprehensive framework for genomic taxonomy of clinical isolates, which can contribute to the unification of global and regional peculiarities of the developing and microevolution of bacterial pathogens.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":"114 49","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-15DOI: 10.29413/abs.2023-8.6.15
M. Dymova, T. A. Shnaider, S. A. Chechetkina, G. O. Petrov, D. O. Malysheva, D. V. Drokov, A. B. Ageenko, N. Vasileva, V. A. Richter, E. Kuligina
Background. One of the promising methods of treating tumors is virotherapy, which is based on direct lysis of cancer cells by a virus and a virus-mediated antitumor immune response of the body. For the recombinant vaccinia virus strain VVGMCSF-Lact, producing human GMCSF and the oncotoxic protein lactaptin, cytotoxic and antitumor effects were shown in experiments in vitro and in vivo, respectively, when using adhesive cultures of U-87 MG human glioblastoma cells. 3D cultures are a more relevant tumor model than adhesive models, as they more fully reflect the realistic scenario of cancer development, as well as the response of the tumor to anticancer therapy.The aim. To evaluate the cytotoxic effect of the oncolytic virus VV-GMCSF-Lact against 3D cultures of human glioblastoma U-87 MG.Materials and methods. The following methods were used in the work: cultivation of 3D cell cultures, cytofluorometry, microscopic analysis, virus titration, statistical analysis.Results. U-87 MG cells were transduced with a lentiviral vector carrying the GFP reporter gene. The cytotoxicity of the VV-GMCSF-Lact virus (IC50) against the studied cells was 0.024 PFU/cell. U-87 MG cells were cultured under conditions for the formation of 3D structures. Microscopic analysis showed the oncolytic effect of the virus on the cells of 3D cultures as early as 24 hours after the start of incubation. Flow cytometry showed an increase in the granularity of glioblastoma cells under the action of the virus, which indicates active replication of the virus in the cells. The virus titer was 0.44 PFU/cell.Conclusions. The recombinant VV-GMCSF-Lact virus has a cytotoxic effect on 3D human glioblastoma U-87 MG cell cultures and actively replicates in them. In the future, to test the oncolytic effect of VV-GMCSF-Lact, it is planned to use not only 3D human glioblastoma cultures, but also cerebral organelles obtained in the process of cocultivation of glioblastoma cells and induced human pluripotent cells.
{"title":"Cytotoxic effect of the VVGMCSF-Lact oncolytic virus against 3D cultures of human glioblastoma cells U-87 MG","authors":"M. Dymova, T. A. Shnaider, S. A. Chechetkina, G. O. Petrov, D. O. Malysheva, D. V. Drokov, A. B. Ageenko, N. Vasileva, V. A. Richter, E. Kuligina","doi":"10.29413/abs.2023-8.6.15","DOIUrl":"https://doi.org/10.29413/abs.2023-8.6.15","url":null,"abstract":"Background. One of the promising methods of treating tumors is virotherapy, which is based on direct lysis of cancer cells by a virus and a virus-mediated antitumor immune response of the body. For the recombinant vaccinia virus strain VVGMCSF-Lact, producing human GMCSF and the oncotoxic protein lactaptin, cytotoxic and antitumor effects were shown in experiments in vitro and in vivo, respectively, when using adhesive cultures of U-87 MG human glioblastoma cells. 3D cultures are a more relevant tumor model than adhesive models, as they more fully reflect the realistic scenario of cancer development, as well as the response of the tumor to anticancer therapy.The aim. To evaluate the cytotoxic effect of the oncolytic virus VV-GMCSF-Lact against 3D cultures of human glioblastoma U-87 MG.Materials and methods. The following methods were used in the work: cultivation of 3D cell cultures, cytofluorometry, microscopic analysis, virus titration, statistical analysis.Results. U-87 MG cells were transduced with a lentiviral vector carrying the GFP reporter gene. The cytotoxicity of the VV-GMCSF-Lact virus (IC50) against the studied cells was 0.024 PFU/cell. U-87 MG cells were cultured under conditions for the formation of 3D structures. Microscopic analysis showed the oncolytic effect of the virus on the cells of 3D cultures as early as 24 hours after the start of incubation. Flow cytometry showed an increase in the granularity of glioblastoma cells under the action of the virus, which indicates active replication of the virus in the cells. The virus titer was 0.44 PFU/cell.Conclusions. The recombinant VV-GMCSF-Lact virus has a cytotoxic effect on 3D human glioblastoma U-87 MG cell cultures and actively replicates in them. In the future, to test the oncolytic effect of VV-GMCSF-Lact, it is planned to use not only 3D human glioblastoma cultures, but also cerebral organelles obtained in the process of cocultivation of glioblastoma cells and induced human pluripotent cells.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":" 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139621624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-15DOI: 10.29413/abs.2023-8.6.13
A.-M. V. Yerofeyeva, S. Pinchuk, S. Rjabceva, A. Molchanova
Objective. To evaluate the anti-nociceptive and reparative effects of adipose-derived mesenchymal stem cells (ADMSCs) under the pharmacological blockade of cannabinoid CB2 receptors in a model of peripheral neuropathic pain.Material and methods. In 40 male Wistar rats, modeling of peripheral neuropathy (NP) was performed by excising a sciatic nerve. On day 7 of the study, ADMSCs (1 × 106 cells/kg) were transplanted into the area of sciatic nerve injury without additional influences or after administration of the CB2 receptor antagonist AM630, as well as after incubation with AM630. Within 90 days, nociceptive sensitivity was studied, as well as a detailed analysis of gait using CatWalk XT (Noldus, Netherlands). On day 21 and day 90, histostructure of the distal segment of the sciatic nerve was assessed.Results. Pharmacological blockade of CB2 receptors both on the ADMSCs and in the soft tissues surrounding the site of sciatic nerve injury led to a decrease in withdrawal threshold and withdrawal latency from day 28 of the study compared with the group of rats with NP and transplantation of ADMSCs only. Local injection of AM630 before transplantation of ADMSCs contributed to the development of NP-induced gait disturbances and increase of the number of damaged nerve fibers in the distal segment of sciatic nerve. Transplantation of ADMSCs pretreated with AM630 did not significantly affect the rate of recovery of gait parameters, and decreased the number of damaged nerve fibers by day 90 of study.Conclusion. Blockade of CB2 receptors, both on the membranes of MSCs and in the area of damage to the peripheral nerve, has a negative effect on the development of the anti-nociceptive and reparative effects of MSCs.
{"title":"Pharmacological blockade of cannabinoid type II receptors and mesenchymal stem cell transplantation in a model of peripheral neuropathic pain","authors":"A.-M. V. Yerofeyeva, S. Pinchuk, S. Rjabceva, A. Molchanova","doi":"10.29413/abs.2023-8.6.13","DOIUrl":"https://doi.org/10.29413/abs.2023-8.6.13","url":null,"abstract":"Objective. To evaluate the anti-nociceptive and reparative effects of adipose-derived mesenchymal stem cells (ADMSCs) under the pharmacological blockade of cannabinoid CB2 receptors in a model of peripheral neuropathic pain.Material and methods. In 40 male Wistar rats, modeling of peripheral neuropathy (NP) was performed by excising a sciatic nerve. On day 7 of the study, ADMSCs (1 × 106 cells/kg) were transplanted into the area of sciatic nerve injury without additional influences or after administration of the CB2 receptor antagonist AM630, as well as after incubation with AM630. Within 90 days, nociceptive sensitivity was studied, as well as a detailed analysis of gait using CatWalk XT (Noldus, Netherlands). On day 21 and day 90, histostructure of the distal segment of the sciatic nerve was assessed.Results. Pharmacological blockade of CB2 receptors both on the ADMSCs and in the soft tissues surrounding the site of sciatic nerve injury led to a decrease in withdrawal threshold and withdrawal latency from day 28 of the study compared with the group of rats with NP and transplantation of ADMSCs only. Local injection of AM630 before transplantation of ADMSCs contributed to the development of NP-induced gait disturbances and increase of the number of damaged nerve fibers in the distal segment of sciatic nerve. Transplantation of ADMSCs pretreated with AM630 did not significantly affect the rate of recovery of gait parameters, and decreased the number of damaged nerve fibers by day 90 of study.Conclusion. Blockade of CB2 receptors, both on the membranes of MSCs and in the area of damage to the peripheral nerve, has a negative effect on the development of the anti-nociceptive and reparative effects of MSCs.","PeriodicalId":505136,"journal":{"name":"Acta Biomedica Scientifica","volume":" 45","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139621788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：