Pub Date : 2024-01-23DOI: 10.1016/j.gde.2024.102152
Kazuhiro Yagita
The circadian clocks are cell-autonomous intrinsic oscillators existing throughout the body to coordinate intracellular and intercellular functions of each organ or tissue. The circadian clock oscillation gradually emerges during mid-to-late gestation in the mammalian developmental process. Recently, it has been revealed that the in vitro differentiation of mouse ES cells recapitulates the circadian clock development. Moreover, reprogramming of the cells results in the redisappearance of the clock, indicating that circadian clocks are tightly coupled with cellular differentiation. Interestingly, before the circadian clock develops, the embryo is governed under ultradian rhythms driven by the segmentation clock. This short review explores these observations, discussing the significance of the emergence of circadian clock oscillation during the mammalian developmental process.
昼夜节律钟是遍布全身的细胞自主固有振荡器,用于协调各器官或组织的细胞内和细胞间功能。在哺乳动物的发育过程中,昼夜节律钟振荡在妊娠中晚期逐渐出现。最近有研究发现,小鼠 ES 细胞的体外分化再现了昼夜节律钟的发育过程。此外,对细胞进行重编程会导致时钟重新出现,这表明昼夜节律钟与细胞分化密切相关。有趣的是,在昼夜节律钟发育之前,胚胎受分割钟驱动的超昼夜节律支配。这篇短文探讨了这些观察结果,讨论了哺乳动物发育过程中出现昼夜节律钟振荡的意义。
{"title":"Emergence of the circadian clock oscillation during the developmental process in mammals","authors":"Kazuhiro Yagita","doi":"10.1016/j.gde.2024.102152","DOIUrl":"10.1016/j.gde.2024.102152","url":null,"abstract":"<div><p>The circadian clocks are cell-autonomous intrinsic oscillators existing throughout the body to coordinate intracellular and intercellular functions of each organ or tissue. The circadian clock oscillation gradually emerges during mid-to-late gestation in the mammalian developmental process. Recently, it has been revealed that the <em>in vitro</em><span> differentiation of mouse ES cells<span><span> recapitulates the circadian clock development. Moreover, reprogramming of the cells results in the redisappearance of the clock, indicating that circadian clocks are tightly coupled with cellular differentiation. Interestingly, before the circadian clock develops, the embryo is governed under </span>ultradian rhythms driven by the segmentation clock. This short review explores these observations, discussing the significance of the emergence of circadian clock oscillation during the mammalian developmental process.</span></span></p></div>","PeriodicalId":50606,"journal":{"name":"Current Opinion in Genetics & Development","volume":"84 ","pages":"Article 102152"},"PeriodicalIF":4.0,"publicationDate":"2024-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139547425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-18DOI: 10.1016/j.gde.2023.102151
Xiao Li , Michael Levine
High-resolution Micro-C maps identified a specialized class of regulatory DNAs termed ‘tethering elements’ (TEs) in Drosophila. These 300–500-bp elements facilitate specific long-range genomic associations or loops. The POZ-containing transcription factor GAF (GAGA-associated factor) contributes to loop formation. Tether–tether interactions accelerate Hox gene activation by distal enhancers, and coordinate transcription of duplicated genes (paralogs) through promoter–promoter associations. Some TEs engage in ultra-long-range enhancer–promoter and promoter–promoter interactions (meta-loops) in the Drosophila brain. We discuss the basis for tether–tether specificity and speculate on the occurrence of similar elements in vertebrate genomes.
{"title":"What are tethering elements?","authors":"Xiao Li , Michael Levine","doi":"10.1016/j.gde.2023.102151","DOIUrl":"https://doi.org/10.1016/j.gde.2023.102151","url":null,"abstract":"<div><p><span>High-resolution Micro-C maps identified a specialized class of regulatory DNAs termed ‘tethering elements’ (TEs) in </span><em>Drosophila</em><span>. These 300–500-bp elements facilitate specific long-range genomic associations or loops. The POZ-containing transcription factor GAF (GAGA-associated factor) contributes to loop formation. Tether–tether interactions accelerate Hox gene activation by distal enhancers, and coordinate transcription of duplicated genes (paralogs) through promoter–promoter associations. Some TEs engage in ultra-long-range enhancer–promoter and promoter–promoter interactions (meta-loops) in the </span><em>Drosophila</em> brain. We discuss the basis for tether–tether specificity and speculate on the occurrence of similar elements in vertebrate genomes.</p></div>","PeriodicalId":50606,"journal":{"name":"Current Opinion in Genetics & Development","volume":"84 ","pages":"Article 102151"},"PeriodicalIF":4.0,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139487306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-12DOI: 10.1016/j.gde.2023.102150
Marie-Noëlle Simon , Karine Dubrana , Benoit Palancade
Nuclear organization has emerged as a critical layer in the coordination of DNA repair activities. Distinct types of DNA lesions have notably been shown to relocate at the vicinity of nuclear pore complexes (NPCs), where specific repair pathways are favored, ultimately safeguarding genome integrity. Here, we review the most recent progress in this field, notably highlighting the increasingly diverse types of DNA structures undergoing repositioning, and the signaling pathways involved. We further discuss our growing knowledge of the molecular mechanisms underlying the choice of repair pathways at NPCs, and their conservation — or divergences. Intriguingly, a series of recent findings suggest that DNA metabolism may be coupled to NPC biogenesis and specialization, challenging our initial vision of these processes.
核组织已成为协调 DNA 修复活动的关键层。研究表明,不同类型的 DNA 病变会迁移到核孔复合体(NPC)附近,而特定的修复途径会在这些地方受到青睐,最终保护基因组的完整性。在此,我们回顾了这一领域的最新进展,特别强调了发生重新定位的 DNA 结构日益多样化的类型,以及所涉及的信号通路。我们还进一步讨论了我们对 NPC 修复途径选择的分子机制及其保护或分歧的日益增长的知识。耐人寻味的是,最近的一系列发现表明,DNA 新陈代谢可能与 NPC 的生物发生和特化相关联,这对我们最初对这些过程的看法提出了挑战。
{"title":"On the edge: how nuclear pore complexes rule genome stability","authors":"Marie-Noëlle Simon , Karine Dubrana , Benoit Palancade","doi":"10.1016/j.gde.2023.102150","DOIUrl":"10.1016/j.gde.2023.102150","url":null,"abstract":"<div><p>Nuclear organization has emerged as a critical layer in the coordination of DNA repair activities. Distinct types of DNA lesions have notably been shown to relocate at the vicinity of nuclear pore complexes (NPCs), where specific repair pathways are favored, ultimately safeguarding genome integrity. Here, we review the most recent progress in this field, notably highlighting the increasingly diverse types of DNA structures undergoing repositioning, and the signaling pathways involved. We further discuss our growing knowledge of the molecular mechanisms underlying the choice of repair pathways at NPCs, and their conservation — or divergences. Intriguingly, a series of recent findings suggest that DNA metabolism may be coupled to NPC biogenesis and specialization, challenging our initial vision of these processes.</p></div>","PeriodicalId":50606,"journal":{"name":"Current Opinion in Genetics & Development","volume":"84 ","pages":"Article 102150"},"PeriodicalIF":4.0,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0959437X23001302/pdfft?md5=08049acdc43be9aa864f08716a109fa2&pid=1-s2.0-S0959437X23001302-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139433196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-09DOI: 10.1016/j.gde.2023.102149
Jorge Lázaro , Jaroslaw Sochacki , Miki Ebisuya
The rate of development is highly variable across animal species. However, the mechanisms regulating developmental tempo have remained elusive due to difficulties in performing direct interspecies comparisons. Here, we discuss how pluripotent stem cell-based models of development can be used to investigate cell- and tissue-autonomous temporal processes. These systems enable quantitative comparisons of different animal species under similar experimental conditions. Moreover, the constantly growing stem cell zoo collection allows the extension of developmental studies to a great number of unconventional species. We argue that the stem cell zoo constitutes a powerful platform to perform comparative studies of developmental tempo, as well as to study other forms of biological time control such as species-specific lifespan, heart rate, and circadian clocks.
{"title":"The stem cell zoo for comparative studies of developmental tempo","authors":"Jorge Lázaro , Jaroslaw Sochacki , Miki Ebisuya","doi":"10.1016/j.gde.2023.102149","DOIUrl":"https://doi.org/10.1016/j.gde.2023.102149","url":null,"abstract":"<div><p>The rate of development is highly variable across animal species. However, the mechanisms regulating developmental tempo have remained elusive due to difficulties in performing direct interspecies comparisons. Here, we discuss how pluripotent stem cell-based models of development can be used to investigate cell- and tissue-autonomous temporal processes. These systems enable quantitative comparisons of different animal species under similar experimental conditions. Moreover, the constantly growing stem cell zoo collection allows the extension of developmental studies to a great number of unconventional species. We argue that the stem cell zoo constitutes a powerful platform to perform comparative studies of developmental tempo, as well as to study other forms of biological time control such as species-specific lifespan, heart rate, and circadian clocks.</p></div>","PeriodicalId":50606,"journal":{"name":"Current Opinion in Genetics & Development","volume":"84 ","pages":"Article 102149"},"PeriodicalIF":4.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0959437X23001296/pdfft?md5=37da80d8b4e7024906d68592591970c7&pid=1-s2.0-S0959437X23001296-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139399337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-04DOI: 10.1016/j.gde.2023.102147
Guanghui Xu , Julie A Law
DNA methylation is a conserved epigenetic modification associated with transposon silencing and gene regulation. The stability of this modification relies on intimate connections between DNA and histone modifications that generate self-reinforcing loops wherein the presence of one mark promotes the other. However, it is becoming increasingly clear that the efficiency of these loops is affected by cross-talk between pathways and by chromatin accessibility, which is heavily influenced by histone variants. Focusing primarily on plants, this review provides an update on the aforementioned self-reinforcing loops, highlights recent advances in understanding how DNA methylation pathways are restricted to prevent encroachment on genes, and discusses the roles of histone variants in compartmentalizing epigenetic pathways within the genome. This multilayered approach facilitates two essential, yet opposing functions, the ability to maintain heritable DNA methylation patterns while retaining the flexibility to modify these patterns during development.
DNA 甲基化是一种保守的表观遗传修饰,与转座子沉默和基因调控有关。这种修饰的稳定性依赖于 DNA 和组蛋白修饰之间的密切联系,这种联系会产生自我强化循环,其中一个标记的存在会促进另一个标记的存在。然而,人们越来越清楚地认识到,这些环路的效率受到途径之间的交叉对话和染色质可及性的影响,而染色质可及性则受到组蛋白变体的严重影响。本综述主要以植物为研究对象,介绍了上述自我强化循环的最新进展,重点介绍了在理解 DNA 甲基化途径如何受到限制以防止侵占基因方面的最新进展,并讨论了组蛋白变体在基因组内表观遗传途径分区中的作用。这种多层次的方法有助于实现两种基本但又相互对立的功能,即维持遗传 DNA 甲基化模式的能力,同时保持在发育过程中修改这些模式的灵活性。
{"title":"Loops, crosstalk, and compartmentalization: it takes many layers to regulate DNA methylation","authors":"Guanghui Xu , Julie A Law","doi":"10.1016/j.gde.2023.102147","DOIUrl":"10.1016/j.gde.2023.102147","url":null,"abstract":"<div><p><span><span>DNA methylation is a conserved epigenetic modification associated with </span>transposon silencing and gene regulation. The stability of this modification relies on intimate connections between DNA and </span>histone modifications<span><span> that generate self-reinforcing loops wherein the presence of one mark promotes the other. However, it is becoming increasingly clear that the efficiency of these loops is affected by cross-talk between pathways and by chromatin accessibility, which is heavily influenced by histone variants. Focusing primarily on plants, this review provides an update on the aforementioned self-reinforcing loops, highlights recent advances in understanding how DNA methylation pathways are restricted to prevent encroachment on genes, and discusses the roles of histone variants in compartmentalizing </span>epigenetic pathways within the genome. This multilayered approach facilitates two essential, yet opposing functions, the ability to maintain heritable DNA methylation patterns while retaining the flexibility to modify these patterns during development.</span></p></div>","PeriodicalId":50606,"journal":{"name":"Current Opinion in Genetics & Development","volume":"84 ","pages":"Article 102147"},"PeriodicalIF":4.0,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139092300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-03DOI: 10.1016/j.gde.2023.102146
Christopher D Balak , Claudia Z Han , Christopher K Glass
Microglia are the major immune cells of the central nervous system (CNS) that perform numerous adaptive functions required for normal CNS development and homeostasis but are also linked to neurodegenerative and behavioral diseases. Microglia development and function are strongly influenced by brain environmental signals that are integrated at the level of transcriptional enhancers to drive specific programs of gene expression. Here, we describe a conceptual framework for how lineage-determining and signal-dependent transcription factors interact to select and regulate the ensembles of enhancers that determine microglia development and function. We then highlight recent findings that advance these concepts and conclude with a consideration of open questions that represent some of the major hurdles to be addressed in the future.
{"title":"Deciphering microglia phenotypes in health and disease","authors":"Christopher D Balak , Claudia Z Han , Christopher K Glass","doi":"10.1016/j.gde.2023.102146","DOIUrl":"10.1016/j.gde.2023.102146","url":null,"abstract":"<div><p>Microglia are the major immune cells of the central nervous system (CNS) that perform numerous adaptive functions required for normal CNS development and homeostasis but are also linked to neurodegenerative and behavioral diseases. Microglia development and function are strongly influenced by brain environmental signals that are integrated at the level of transcriptional enhancers to drive specific programs of gene expression. Here, we describe a conceptual framework for how lineage-determining and signal-dependent transcription factors interact to select and regulate the ensembles of enhancers that determine microglia development and function. We then highlight recent findings that advance these concepts and conclude with a consideration of open questions that represent some of the major hurdles to be addressed in the future.</p></div>","PeriodicalId":50606,"journal":{"name":"Current Opinion in Genetics & Development","volume":"84 ","pages":"Article 102146"},"PeriodicalIF":4.0,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0959437X23001260/pdfft?md5=d9b6062b0d6039a595753d6b3ef4cab2&pid=1-s2.0-S0959437X23001260-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139077552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.1016/j.gde.2023.102136
Pedro Avila-Lopez , Shannon M Lauberth
A significant portion of the human proteome comprises RNA-binding proteins (RBPs) that play fundamental roles in numerous biological processes. In the last decade, there has been a staggering increase in RBP identification and classification, which has fueled interest in the evolving roles of RBPs and RBP-driven molecular mechanisms. Here, we focus on recent insights into RBP-dependent regulation of the epigenetic and transcriptional landscape. We describe advances in methodologies that define the RNA-protein interactome and machine-learning algorithms that are streamlining RBP discovery and predicting new RNA-binding regions. Finally, we present how RBP dysregulation leads to alterations in tumor-promoting gene expression and discuss the potential for targeting these RBPs for the development of new cancer therapeutics.
{"title":"Exploring new roles for RNA-binding proteins in epigenetic and gene regulation","authors":"Pedro Avila-Lopez , Shannon M Lauberth","doi":"10.1016/j.gde.2023.102136","DOIUrl":"10.1016/j.gde.2023.102136","url":null,"abstract":"<div><p><span>A significant portion of the human proteome comprises RNA-binding proteins (RBPs) that play fundamental roles in numerous </span>biological processes<span><span>. In the last decade, there has been a staggering increase in RBP identification and classification, which has fueled interest in the evolving roles of RBPs and RBP-driven molecular mechanisms. Here, we focus on recent insights into RBP-dependent regulation of the epigenetic and transcriptional landscape. We describe advances in methodologies that define the RNA-protein </span>interactome and machine-learning algorithms that are streamlining RBP discovery and predicting new RNA-binding regions. Finally, we present how RBP dysregulation leads to alterations in tumor-promoting gene expression and discuss the potential for targeting these RBPs for the development of new cancer therapeutics.</span></p></div>","PeriodicalId":50606,"journal":{"name":"Current Opinion in Genetics & Development","volume":"84 ","pages":"Article 102136"},"PeriodicalIF":4.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138832830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-12DOI: 10.1016/j.gde.2023.102137
Hina Bharti , Sungwook Han , Han-Wen Chang , Danny Reinberg
Epigenetic reprogramming during development is key to cell identity and the activities of the Polycomb repressive complexes are vital for this process. We focus on polycomb repressive complex 2 (PRC2), which catalyzes H3K27me1/2/3 and safeguards cellular integrity by ensuring proper gene repression. Notably, various accessory factors associate with PRC2, strongly influencing cell fate decisions, and their deregulation contributes to various illnesses. Yet, the exact role of these factors during development and carcinogenesis is not fully understood. Here, we present recent progress toward addressing these points and an analysis of the expression levels of PRC2 accessory factors in various tissues and developmental stages to highlight their abundance and roles. Last, we evaluate their contribution to cancer-specific phenotypes, providing insight into novel anticancer therapies.
{"title":"Polycomb repressive complex 2 accessory factors: rheostats for cell fate decision?","authors":"Hina Bharti , Sungwook Han , Han-Wen Chang , Danny Reinberg","doi":"10.1016/j.gde.2023.102137","DOIUrl":"10.1016/j.gde.2023.102137","url":null,"abstract":"<div><p>Epigenetic reprogramming during development is key to cell identity and the activities of the Polycomb repressive complexes are vital for this process. We focus on polycomb repressive complex 2 (PRC2), which catalyzes H3K27me1/2/3 and safeguards cellular integrity by ensuring proper gene repression. Notably, various accessory factors associate with PRC2, strongly influencing cell fate decisions, and their deregulation contributes to various illnesses. Yet, the exact role of these factors during development and carcinogenesis is not fully understood. Here, we present recent progress toward addressing these points and an analysis of the expression levels of PRC2 accessory factors in various tissues and developmental stages to highlight their abundance and roles. Last, we evaluate their contribution to cancer-specific phenotypes, providing insight into novel anticancer therapies.</p></div>","PeriodicalId":50606,"journal":{"name":"Current Opinion in Genetics & Development","volume":"84 ","pages":"Article 102137"},"PeriodicalIF":4.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0959437X2300117X/pdfft?md5=c90607690ced8c29da1feecf9cf354e3&pid=1-s2.0-S0959437X2300117X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138576205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-05DOI: 10.1016/j.gde.2023.102134
Charlotte E Handford , Sergi Junyent , Victoria Jorgensen , Magdalena Zernicka-Goetz
Stem cell-based mammalian embryo models facilitate the discovery of developmental mechanisms because they are more amenable to genetic and epigenetic perturbations than natural embryos. Here, we highlight exciting recent advances that have yielded a plethora of models of embryonic development. Imperfections in these models highlight gaps in our current understanding and outline future research directions, ushering in an exciting new era for embryology.
{"title":"Topical section: embryonic models (2023) for Current Opinion in Genetics & Development","authors":"Charlotte E Handford , Sergi Junyent , Victoria Jorgensen , Magdalena Zernicka-Goetz","doi":"10.1016/j.gde.2023.102134","DOIUrl":"10.1016/j.gde.2023.102134","url":null,"abstract":"<div><p><span>Stem cell-based mammalian embryo models facilitate the discovery of developmental mechanisms because they are more amenable to genetic<span> and epigenetic perturbations than natural embryos. Here, we highlight exciting recent advances that have yielded a plethora of models of </span></span>embryonic development. Imperfections in these models highlight gaps in our current understanding and outline future research directions, ushering in an exciting new era for embryology.</p></div>","PeriodicalId":50606,"journal":{"name":"Current Opinion in Genetics & Development","volume":"84 ","pages":"Article 102134"},"PeriodicalIF":4.0,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138488971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-04DOI: 10.1016/j.gde.2023.102135
Xiaodong Liu , Jose M Polo
Human development is a highly coordinated process, with any abnormalities during the early embryonic stages that can often have detrimental consequences. The complexity and nuances of human development underpin its significance in embryo research. However, this research is often hindered by limited availability and ethical considerations associated with the use of donated blastocysts from in vitro fertilization (IVF) surplus. Human blastoids offer promising alternatives as they can be easily generated and manipulated in the laboratory while preserving key characteristics of human blastocysts. In this way, they hold the potential to serve as a scalable and ethically permissible resource in embryology research. By utilizing such human embryo models, we can establish a transformative platform that complements the study with IVF embryos, ultimately enhancing our understanding of human embryogenesis.
{"title":"Human blastoid as an in vitro model of human blastocysts","authors":"Xiaodong Liu , Jose M Polo","doi":"10.1016/j.gde.2023.102135","DOIUrl":"10.1016/j.gde.2023.102135","url":null,"abstract":"<div><p>Human development is a highly coordinated process, with any abnormalities during the early embryonic stages that can often have detrimental consequences. The complexity and nuances of human development underpin its significance in embryo research. However, this research is often hindered by limited availability and ethical considerations associated with the use of donated blastocysts from <em>in vitro</em> fertilization (IVF) surplus. Human blastoids offer promising alternatives as they can be easily generated and manipulated in the laboratory while preserving key characteristics of human blastocysts. In this way, they hold the potential to serve as a scalable and ethically permissible resource in embryology research. By utilizing such human embryo models, we can establish a transformative platform that complements the study with IVF embryos, ultimately enhancing our understanding of human embryogenesis.</p></div>","PeriodicalId":50606,"journal":{"name":"Current Opinion in Genetics & Development","volume":"84 ","pages":"Article 102135"},"PeriodicalIF":4.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0959437X23001156/pdfft?md5=9a6eae3b487a1baccf0554d4dd293f3a&pid=1-s2.0-S0959437X23001156-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138488970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}