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Extraction of Soluble Polysaccharides from Grape Skins 葡萄皮中可溶性多糖的提取
Q2 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.7764/RCIA.V44I1.1709
Álvaro Peña Neira, M. G. I. Cortiella
Obtaining polysaccharides from grape cell walls is an interesting topic for the wine industry given its implications with regards to wine treatments and potential sensory attributes. In the present work, several conditions (i.e., a chelating agent, pH, titratable acidity, extraction time, number of successive extractions, solid to liquid ratio and alcoholic fermentation) were tested to assess their effects on the yield of soluble polysaccharides obtained from grape skins. Among the four tested solvents (i.e., water, tartaric acid, ammonium oxalate, and ethylenediaminetetraacetic acid), aqueous ammonium oxalate was the most effective for polysaccharide extraction but also produced the highest amount of protein impurities. Increasing the number of successive extractions or modifying the extraction time does not increase the amount of total extracted polysaccharides but produces more polysaccharides with a low molecular mass. The extraction yield of polysaccharides does not increase by increasing the tartaric acid concentration; however, the extraction yield increases by increasing the pH of the solvent or decreasing the solid to liquid ratio during the extraction. During the fermentative maceration of the skins, the extraction of soluble polysaccharides is significantly higher than that obtained with aqueous solvents. Thus, it seems that grape skins contain some pectic polysaccharides that are tightly bound to the cell wall matrix that are not immediately solubilized by aqueous buffers and are released under alcoholic fermentation conditions.
从葡萄细胞壁中提取多糖对于葡萄酒行业来说是一个有趣的话题,因为它涉及到葡萄酒的处理和潜在的感官属性。在本工作中,测试了几种条件(即螯合剂、pH、可滴定酸度、提取时间、连续提取次数、料液比和酒精发酵),以评估它们对葡萄皮中可溶性多糖得率的影响。在四种溶剂(水、酒石酸、草酸铵和乙二胺四乙酸)中,草酸铵水溶液对多糖的提取效果最好,但产生的蛋白质杂质也最多。增加连续提取次数或改变提取时间并不会增加总多糖的提取量,但会产生更多低分子质量的多糖。酒石酸浓度的增加不增加多糖的提取率;然而,在提取过程中,通过增加溶剂的pH值或降低料液比可以提高提取率。在皮的发酵浸渍过程中,可溶性多糖的提取率明显高于水溶剂的提取率。因此,葡萄皮似乎含有一些果胶多糖,这些多糖与细胞壁基质紧密结合,不能立即被水缓冲液溶解,并在酒精发酵条件下释放出来。
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引用次数: 8
Nutritional Composition and Polyphenolic Content of Edible Peumus boldus Mol. Fruits. 可食性山茱萸果实的营养成分及多酚含量。
Q2 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.7764/RCIA.V44I1.1684
A. Giordano, Gloria Montengero, P. Velásquez, M. Gómez, Ignacia Sandoval
Peumus boldus is an endemic Chilean tree whose edible fruit lack a history of scientific research. The aim of this study was to determine its nutritional composition (moisture, ash, crude fiber, proteins, carbohydrates and lipids) and polyphenolic content (total phenolic content, HPLC-DAD, HPLC-MS/MS analysis) at different stages of development. The results show that the fruit decreased in ash, crude fiber and total phenolic content throughout its development, while its carbohydrate and protein content increased. An increase in phenolic molecules such as catechin and epicatechin was also observed. This is the first report on the nutritional composition and phenolic content of Peumus boldus Mol. fruits.
红椒是智利特有的树种,其可食用果实缺乏科学研究的历史。本研究的目的是测定其不同发育阶段的营养成分(水分、灰分、粗纤维、蛋白质、碳水化合物和脂类)和多酚含量(总酚含量、HPLC-DAD、HPLC-MS/MS分析)。结果表明:果实在发育过程中灰分、粗纤维和总酚含量呈下降趋势,而碳水化合物和蛋白质含量呈上升趋势;儿茶素和表儿茶素等酚类分子也有所增加。本文首次报道了红杜鹃花果实的营养成分和酚类物质含量。
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引用次数: 0
USLE/RUSLE K-factors allocated through a linear mixed model for Uruguayan soils USLE/RUSLE k因子通过乌拉圭土壤的线性混合模型分配
Q2 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.7764/RCIA.V44I1.1622
A. Beretta-Blanco, L. Carrasco-Letelier
Soil erosion by rainfall is a process that demands management, both for the prevention of excessive soil erosion and for the protection of the quality of freshwater bodies. Erosion coefficients (K-factors) of the universal soil loss equation (USLE)/revised USLE (RUSLE) model were assigned to 99 mapped Uruguayan soil types at 1:1,000,000 scale. This work developed a linear mixed model (LMM) with 79 soils with assigned K-factors, in which the following variables were considered: soil taxonomy, chemical composition, and parent material. The developed LMM had an R2=0.86, in which the soil taxonomy (p<0.0001), parent material (p=0.0174), clay (p=0.0005) and sand (p=0.017) contents had significant statistical effects. The prediction capacity of this model was assessed with 10 soils not previously used in development of the LMM with assigned K-factors. The prediction assessment had an R2=0.84 and a mean error of 9.08% of the mean K-factor value. The LMM developed was used for the allocation of K-factors to soils mapped at a 1:20,000-resolution. Thus, the use of LMM increased the soil area with assigned K-factors from 111,822 km2 (at a scale of 1:1,000,000) to 174,132 km2 (1:20,000).
降雨造成的土壤侵蚀是一个需要管理的过程,既要防止土壤过度侵蚀,又要保护淡水水体的质量。将通用土壤流失方程(USLE)/修正USLE (RUSLE)模型的侵蚀系数(k因子)按1:10万比例尺分配给99个绘制的乌拉圭土壤类型。本工作建立了一个线性混合模型(LMM),其中79种土壤具有指定的k因子,其中考虑了以下变量:土壤分类、化学成分和母质。发达LMM的R2=0.86,其中土壤分类(p<0.0001)、母质(p=0.0174)、粘土(p=0.0005)和砂(p=0.017)含量具有显著的统计学影响。该模型的预测能力用10种土壤进行了评估,这些土壤以前没有用于开发具有指定k因子的LMM。预测评价的R2=0.84,平均误差为k因子平均值的9.08%。开发的LMM用于按1:20 000分辨率对土壤进行k因子分配。因此,LMM的使用使分配k因子的土壤面积从111,822 km2(1:10,000)增加到174,132 km2(1:20 000)。
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引用次数: 9
Genetic parameters for milk yield and reproduction traits in the Chilean Dairy Overo Colorado cattle breed 智利奥弗罗-科罗拉多奶牛品种产奶量和繁殖性状的遗传参数
Q2 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.7764/RCIA.V44I1.1562
H. Montaldo, C. Trejo, C. Lizana
The objective of this study was to estimate genetic parameters for milk production and reproduction traits, with phenotypic and pedigree information from the Dairy Overo Colorado breed from southern Chile. Single and multi-trait mixed models were used to analyze the data. Analyses were performed using ASReml software. Heritability estimates for milk yield, fat yield, protein yield, fat content, protein content, age at first calving and calving interval, were 0.29, 0.34, 0.30, 0.56, 0.56, 0.01 and 0.002, respectively. Genetic correlations between yield traits were high and positive (≥ 0.81). Genetic correlations of milk yield with fat content (-0.32) and protein content (-0.30) were unfavorable. Genetic correlation estimates between age at first calving and yield traits were negative between -0.71 and -0.29. Phenotypic correlations between calving interval and milk yield traits were slightly positive (0.06-0.09), suggesting a weak relationship between reproduction and yield in this population. Genetic variation is considerable for milk production traits. Genetic improvement for age at first calving and calving interval may be slow, because small differences between animals and low reliabilities are expected for estimated breeding values for these traits due to low heritabilities. The design of an efficient breeding program for yield traits based on artificial insemination for this population is feasible using either progeny testing or genomic breeding value prediction for selecting sires.
本研究的目的是利用来自智利南部的Dairy Overo Colorado品种的表型和系谱信息,估计产奶和繁殖性状的遗传参数。采用单性状和多性状混合模型对数据进行分析。使用ASReml软件进行分析。产奶量、脂肪产量、蛋白质产量、脂肪含量、蛋白质含量、初产龄和产犊间隔的遗传力分别为0.29、0.34、0.30、0.56、0.56、0.01和0.002。产量性状间呈高正相关(≥0.81)。产奶量与脂肪含量(-0.32)和蛋白质含量(-0.30)的遗传相关不显著。初产犊龄与产量性状的遗传相关在-0.71 ~ -0.29之间呈负相关。产犊间隔与产奶量性状呈微正相关(0.06 ~ 0.09),表明该种群的繁殖与产奶量之间存在较弱的相关关系。产奶性状的遗传变异是相当大的。首次产犊年龄和产犊间隔的遗传改进可能很慢,因为动物之间的差异很小,而且由于遗传力低,这些性状的估计育种值的可靠性很低。采用子代检测或基因组育种价值预测方法进行选种,为该群体设计高效的人工授精产量性状育种方案是可行的。
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引用次数: 10
Effect of crop load on the phenological, vegetative and reproductive behavior of the ‘Frantoio’ olive tree (Olea europaea L.) 作物负荷对‘Frantoio’橄榄树物候、营养和生殖行为的影响
Q2 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.7764/RCIA.V44I1.1653
Victor Beyá, T. Fichet
The aim of this study was to characterize the phenological, vegetative and reproductive behavior variables of ‘Frantoio’ olive trees in an “off” year (with a low crop load) and an “on” year (with a high crop load). To do this, during the 2011–2012 and 2012–2013 seasons, “off” and “on”, respectively, phenological variables, plant growth (shoots and roots) and reproductive growth (type of flower, fruit set and growth), were monitored biweekly. In addition, the yield per tree was assessed, and the crop load was estimated. The main results showed that crop load strongly influenced these variables. The distribution and intensity of vegetative growth, both in the roots and aboveground, decreased more and had lower intensity in the high fruiting season, with a large part of this growth occurring prior to flowering. With respect to reproductive variables, a higher percentage of perfect flowers and fruit set in the low flowering season was observed compared to the high load season. These results suggest a partial crop load compensation mechanism under low flowering conditions. This mechanism, however, does not compensate for lower production in the “off” season. In terms of volume, fruit growth was similar between seasons until pit hardening, then the fruit growth rate dropped dramatically in the “on” season, indicating that fruit growth, until pit hardening, would not be affected by the presence of a higher number of fruits per tree.
本研究的目的是表征' Frantoio '橄榄树在“off”年(低作物负荷)和“on”年(高作物负荷)的物候,营养和生殖行为变量。为此,在2011-2012年和2012-2013年两季,分别对物候变量、植物生长(芽和根)和生殖生长(花的类型、坐果和生长)进行“关闭”和“打开”监测。此外,还评估了单株产量和作物负荷。主要结果表明,作物负荷对这些变量的影响较大。在结实季,根系和地上营养生长的分布和强度均下降较多,且强度较低,且大部分生长发生在开花前。在生殖变量方面,低花期完花率和坐果率均高于高花期。这些结果提示在低花期条件下存在部分作物负荷补偿机制。然而,这种机制并不能弥补“淡季”产量的下降。就体积而言,果实在果核硬化之前的生长在季节之间是相似的,然后果实生长速度在“开启”季节急剧下降,这表明在果核硬化之前,果实生长不会受到较高的单株果实数量的影响。
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引用次数: 5
Increasing the shelf life of post-harvest table grapes (Vitis vinífera cv. Thompson Seedless) using different packaging material with copper nanoparticles to change the atmosphere 增加收获后鲜食葡萄的保质期(Vitis vinífera cv。汤普森无籽)使用不同的包装材料与铜纳米粒子来改变大气
Q2 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.7764/RCIA.V44I1.1640
N. Loyola, M. Arriola
An effective way to control post-harvest rotting of table grapes is the use of sulfur dioxide (SO2). However, under certain conditions, the use of SO2 can cause significant loss, mainly associated with bleaching of the berries. Therefore, it is believed that the use of bags with and without copper nanoparticles that modify the atmosphere will allow for the control of Botrytis cinerea as well as the ability to dispense with the use of sulfur dioxide generation. The objective of this test is to evaluate the use of bags with and without copper nanoparticles that modify atmosphere and to evaluate their ability to control post-harvest B. cinerea, as well as their effect on the conservation of physical and chemical characteristics of table grapes. The results indicate that the use of air bags with or without copper nanoparticles that modify the atmosphere and do not require sulfur dioxide generation were able to control B. cinerea post-harvest and preserve the physical and chemical parameters of grape table cv. ‘Thompson seedless’. Significant differences were seen in parameters such as whitening, dehydration and incidence of stalk rot. A group of panelists could not distinguish differences in the sensory attributes and acceptability of the grapes regardless of treatment.
控制鲜食葡萄采后腐烂的有效方法是使用二氧化硫(SO2)。然而,在一定条件下,SO2的使用会造成重大损失,主要与浆果的漂白有关。因此,人们相信,使用带有或不带有铜纳米粒子的袋子来改变大气,将允许控制灰葡萄孢菌,以及免除使用二氧化硫生成的能力。本试验的目的是评价带有和不带有铜纳米粒子的袋子的使用,这些纳米粒子可以改变空气,并评价它们控制采后灰葡萄球菌的能力,以及它们对保存鲜食葡萄的物理和化学特性的影响。结果表明,使用含或不含铜纳米粒子的气囊,可以改变大气,不需要产生二氧化硫,可以控制葡萄采后的葡萄球菌,并保存葡萄表cv的物理和化学参数。“汤普森无核”。在美白、脱水和茎腐病发生率等参数上可以看到显著差异。一组小组成员无法区分葡萄的感官属性和可接受性的差异,无论处理如何。
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引用次数: 1
Storage, size and vigor of ‘Esther’ avocado seeds (Persea americana Mill.) “埃斯特”鳄梨种子的储存、大小和活力(Persea americana Mill)
Q2 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.7764/RCIA.V44I1.1641
Luisa Gálvez-Cendegui, P. Peñaloza, E. Oyanedel, M. Castro
Avocado seeds present high size variability. Larger seeds, such those of the ‘Esther’ variety, are preferred when nurse seeds are used. Even though they are considered recalcitrant seeds, they are stored for propagation. The aim of this study was to evaluate the combined effect of three storage periods and three sizes of ‘Esther’ avocado seeds on vigor, in terms of electrolyte leakage, and seedling length and diameter. The experiment was conducted using a completely randomized factorial design. The factors were time in storage (10, 30 and 100 d at 5 °C and 80% relative humidity) and seed size (small, medium and large). Electrolyte leakage was evaluated as a measure of seed vigor, growth and later development of the seedlings. Seeds stored for 30 and 100 d showed the highest electrolyte leakage but produced seedlings with a larger stem diameter than 10-day-old seeds. The seeds stored for 100 d generated seedlings with stems three times wider than the seedlings stored for 10 d. The changes observed in electrical conductivity showed differences between the different sizes and storage times of seeds. It is concluded that storage time had a positive effect on the growth of ‘Esther’ avocado seedlings (length and diameter of the stem) regardless of the seed size. Electrolyte leakage was affected by seed age and seed size but did not correlate with subsequent seedling growth.
鳄梨种子具有高度的大小可变性。当使用护士种子时,更大的种子,如“以斯帖”品种的种子是首选。尽管它们被认为是顽固的种子,但它们被储存起来用于繁殖。本研究的目的是评价3种贮藏期和3种尺寸对‘Esther’鳄梨种子活力、电解质泄漏、幼苗长和直径的综合影响。本试验采用完全随机因子设计。影响因素为贮藏时间(5℃、80%相对湿度条件下10、30和100 d)和种子大小(小、中、大)。电解质泄漏是衡量种子活力、幼苗生长和后期发育的指标。储存30 d和100 d的种子电解质泄漏量最大,但产生的幼苗茎粗大于10 d的种子。贮藏100 d的种子产生的幼苗茎宽是贮藏10 d的3倍。电导率的变化表明不同大小和贮藏时间的种子存在差异。综上所述,无论种子大小,贮藏时间对‘Esther’鳄梨幼苗的生长(茎长和茎粗)都有积极影响。电解质泄漏受种子龄和种子大小的影响,但与随后的幼苗生长无关。
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引用次数: 2
Agronomic characteristics and chemical composition of Gliricidia sepium grown under different residual heights in different seasons 不同季节不同残差高度下金菖蒲的农艺特征及化学成分
Q2 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.7764/RCIA.V44I1.1579
S. Ferreira, M. S. D. S. Carneiro, R. Edvan, E. Pereira, L. D. M. Neto, A. P. Pinto, D. Camilo
This research was conducted to evaluate the agronomic characteristics and chemical composition of Gliricidia sepium grown under different residual heights in different seasons. Experimental design used was a complete randomized block in a split-plot. Plots consisted of three residual heights (70, 90 and 110 cm) and subplots were four seasons (fall, winter, spring and summer) with four replications. There was no effect (P>0.05) of interaction between season and residual heights for agronomic characteristics. However, these features were influenced by the cutting season, with no significant effect (P>0.05) for residual height significant effects (P<0.05) were observed for DM, MM, ADF and EE in cutting seasons and residual height. Gliricidia sepium can be cut in residual height of 70 cm, with better agronomic characteristics and chemical composition in fall.
本研究对不同季节不同残差高度下种植的水杨花的农艺性状和化学成分进行了评价。采用的实验设计是一个完全随机分组的分裂图。样地由3个残差高度(70、90和110 cm)组成,子样地分为4个季节(秋、冬、春、夏),共4个重复。季节与残高互作对农艺性状无显著影响(P>0.05)。但这些特征均受采伐季节的影响,对残高无显著影响(P>0.05)。DM、MM、ADF和EE在采伐季节和残高均有显著影响(P<0.05)。秋砍残高可达70 cm,具有较好的农艺性状和化学成分。
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引用次数: 9
Comparison of chemical composition and anti-methanogenic potential of Liquidambar orientalis leaves with Laurus nobilis and Eucalyptus globulus leaves using in vitro gas production technique 利用体外产气技术比较东方野檀与月桂、蓝桉叶的化学成分及抗甲烷潜能
Q2 Agricultural and Biological Sciences Pub Date : 2017-01-01 DOI: 10.7764/RCIA.V44I1.1637
İ. Ülger, A. Kamalak, E. Kaya, Ö. Kurt
The aim of the present study was to compare the leaves of Liquidambar orientalis with leaves of Laurus nobilis and Eucalyptus globulus in terms of chemical composition and anti-methanogenic potentials using the in vitro gas production technique. Species had a significant effect on the chemical composition, gas production, methane production, metabolisable energy and organic matter digestibility of tree leaves. Crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and condensed tannin (CT) contents of tree leaves ranged from 9.11 to 12.8, 22.49 to 35.85 and 32.76 to 49.31, 1.55 to 9.29% respectively. Gas production, methane production, metabolisable energy (ME) and organic matter digestibility (OMD) ranged from 21.72 to 31.54 ml, 2.62 ml to 4.41 ml, 6.62 to 9.24 MJ /kg DM, 41.23 to 54.84 % respectively. It is likely that leaves of Liquidambar orientalis will be more effective for methane mitigation in ruminant animals as compared with Eucalyptus globules and Laurus nobilis. However before large implication it should be tested in vivo to evaluate the effect of leaves of Liquidambar orientalis on animal production
摘要利用体外产气技术,比较了东方枫叶、月桂叶和蓝桉叶的化学成分和抗甲烷活性。树种对树木叶片的化学成分、产气量、甲烷产量、代谢能和有机质消化率有显著影响。粗蛋白质(CP)、中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)和缩合单宁(CT)含量分别为9.11 ~ 12.8%、22.49 ~ 35.85、32.76 ~ 49.31、1.55 ~ 9.29%。产气量、产甲烷量、代谢能(ME)和有机物消化率(OMD)分别为21.72 ~ 31.54 ml、2.62 ~ 4.41 ml、6.62 ~ 9.24 MJ /kg DM、41.23 ~ 54.84%。与桉叶和月桂叶相比,东方枫叶对反刍动物体内甲烷的缓解作用可能更有效。但在此之前,还需进行动物体内试验,以评估东洋枫叶对动物生产的影响
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引用次数: 8
Rendimiento, características del rendimiento, composición fitoquímico, actividad antioxidante y antibacteriana del Abutilon indicum cultivado con diferentes formas de aplicación de fertilizantes 不同施肥方式培养的靛蓝的产量、产量特性、植物化学成分、抗氧化和抗菌活性
Q2 Agricultural and Biological Sciences Pub Date : 2016-12-01 DOI: 10.4067/S0718-16202016000300012
Gulsum Yaldiz, Arzu Birinci Yildirim, Yeliz Kasko Arici, Mahmut Çamlica
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引用次数: 0
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Ciencia E Investigacion Agraria
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