A. Seyedzadeh, Z. Rahimi, M. Tohidi, Nargece Soraya
Background: Urolithiasis is the most prevalent uronephrologic disorder caused by genetic, environmental factors, and metabolic defects. Objectives: The present study aimed to find a possible association between the vitamin D receptor (VDR) TaqI and the BsmI gene polymorphisms in relation to the serum levels of calcium (Ca) and vitamin D and the risk of urolithiasis. Methods: This case-control study was conducted on 68 children with urolithiasis and 67 healthy controls for the VDR gene polymorphisms using the polymerase chain reaction-restriction length polymorphism method. Results: The frequency of TaqI C allele was 36% in patients compared to 32.1% in controls (P = 0.49). A significantly higher frequency of the TaqI CC genotype was found among patients in the age group > 5 to 10 years. No significant difference was detected in the frequency of the BsmI A allele between patients (41.9%) and controls (42.5%, P = 0.91). However, a significantly lower frequency of the BsmI AA genotype was detected compared to those patients with Ca levels of > 10.8 mg/dL among patients with Ca levels of ≤ 10.8 mg/dL. Conclusions: Based on the results, a lack of an association between the VDR TaqI and BsmI polymorphisms with the risk of urolithiasis among children from Western Iran. However, a higher frequency of the TaqI CC genotype was found in the age group > 5 to 10 years. In addition, lower levels of Ca in patients were related to a significantly lower frequency of the BsmI AA genotype. The VDR gene BsmI polymorphism might affect the calcium level and the calcium metabolism in urolithiasis.
背景：尿路结石是由遗传、环境因素和代谢缺陷引起的最常见的泌尿生殖系统疾病。研究目的本研究旨在发现维生素 D 受体（VDR）TaqI 和 BsmI 基因多态性与血清钙（Ca）和维生素 D 水平及尿路结石风险之间可能存在的关联。研究方法本病例对照研究采用聚合酶链式反应-限制长度多态性方法，对 68 名尿路结石患儿和 67 名健康对照者进行了 VDR 基因多态性检测。结果显示患者的 TaqI C 等位基因频率为 36%，而对照组为 32.1%（P = 0.49）。在年龄大于 5 至 10 岁的患者中，TaqI CC 基因型的频率明显较高。患者（41.9%）和对照组（42.5%，P = 0.91）的 BsmI A 等位基因频率没有明显差异。然而，与血钙值大于 10.8 mg/dL 的患者相比，血钙值小于 10.8 mg/dL 的患者的 BsmI AA 基因型频率明显较低。结论根据研究结果，伊朗西部儿童的 VDR TaqI 和 BsmI 多态性与罹患尿石症的风险没有关联。不过，在 5 至 10 岁年龄组中，TaqI CC 基因型的频率较高。此外，患者体内钙含量较低与 BsmI AA 基因型频率明显较低有关。VDR基因BsmI多态性可能会影响尿石症患者的血钙水平和钙代谢。
{"title":"Gene Variants of Vitamin D Receptor (TaqI T > C (rs731236) and BsmI G > A (rs1544410) in Urolithiasis","authors":"A. Seyedzadeh, Z. Rahimi, M. Tohidi, Nargece Soraya","doi":"10.5812/jkums-143931","DOIUrl":"https://doi.org/10.5812/jkums-143931","url":null,"abstract":"Background: Urolithiasis is the most prevalent uronephrologic disorder caused by genetic, environmental factors, and metabolic defects. Objectives: The present study aimed to find a possible association between the vitamin D receptor (VDR) TaqI and the BsmI gene polymorphisms in relation to the serum levels of calcium (Ca) and vitamin D and the risk of urolithiasis. Methods: This case-control study was conducted on 68 children with urolithiasis and 67 healthy controls for the VDR gene polymorphisms using the polymerase chain reaction-restriction length polymorphism method. Results: The frequency of TaqI C allele was 36% in patients compared to 32.1% in controls (P = 0.49). A significantly higher frequency of the TaqI CC genotype was found among patients in the age group > 5 to 10 years. No significant difference was detected in the frequency of the BsmI A allele between patients (41.9%) and controls (42.5%, P = 0.91). However, a significantly lower frequency of the BsmI AA genotype was detected compared to those patients with Ca levels of > 10.8 mg/dL among patients with Ca levels of ≤ 10.8 mg/dL. Conclusions: Based on the results, a lack of an association between the VDR TaqI and BsmI polymorphisms with the risk of urolithiasis among children from Western Iran. However, a higher frequency of the TaqI CC genotype was found in the age group > 5 to 10 years. In addition, lower levels of Ca in patients were related to a significantly lower frequency of the BsmI AA genotype. The VDR gene BsmI polymorphism might affect the calcium level and the calcium metabolism in urolithiasis.","PeriodicalId":507382,"journal":{"name":"Journal of Kermanshah University of Medical Sciences","volume":"120 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141682041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Context: The widespread use of biocides containing quaternary ammonium compounds (QACs) in medical centers has led to resistant strains of microorganisms. This study aimed to evaluate the frequency of genes resistant to antiseptics in Iranian bacterial isolates. Methods: The present study was conducted as a systematic review. The keywords in the titles or abstracts of articles published in Persian and English were monitored. The search was performed in databases without a time limit. Results: The results showed a total of 975 bacterial isolates. The most common disinfectant-resistant genes in gram-positive isolates were QacAB genes, with an average of 28.48; SMR gene, with an average of 19.8; and qacE and qacEΔ1 genes, with an average of 45 in gram-negative isolates. Gram-negative isolates containing qacE and qacEΔ1 genes were mainly detected in patients hospitalized in intensive care, internal diseases, and infectious diseases wards and from trachea and urine samples. Conclusions: The unique conditions of the intensive care units and the use of invasive tools such as urinary catheters were the leading causes of infection with resistant pathogens. In addition, diversity in infection control measures like the type, amount, and concentration of disinfectants used in different hospital departments were other reasons for resistance to antiseptics in medical environments. Indiscriminate use of antibiotics caused microbial resistance in the same way antibiotics did not affect many microbial diseases, and arbitrary use caused the highest microbial resistance.
{"title":"Prevalence of Antibiotic Resistance Genes in Bacterial Isolates in Iran: A Systematic Review","authors":"MohammadHosein Zamanian, Armin Naghipour, Zainab Mohseni Afshar, Negar Pourtaieb, Zahra Naghibifar","doi":"10.5812/jkums-139147","DOIUrl":"https://doi.org/10.5812/jkums-139147","url":null,"abstract":"Context: The widespread use of biocides containing quaternary ammonium compounds (QACs) in medical centers has led to resistant strains of microorganisms. This study aimed to evaluate the frequency of genes resistant to antiseptics in Iranian bacterial isolates. Methods: The present study was conducted as a systematic review. The keywords in the titles or abstracts of articles published in Persian and English were monitored. The search was performed in databases without a time limit. Results: The results showed a total of 975 bacterial isolates. The most common disinfectant-resistant genes in gram-positive isolates were QacAB genes, with an average of 28.48; SMR gene, with an average of 19.8; and qacE and qacEΔ1 genes, with an average of 45 in gram-negative isolates. Gram-negative isolates containing qacE and qacEΔ1 genes were mainly detected in patients hospitalized in intensive care, internal diseases, and infectious diseases wards and from trachea and urine samples. Conclusions: The unique conditions of the intensive care units and the use of invasive tools such as urinary catheters were the leading causes of infection with resistant pathogens. In addition, diversity in infection control measures like the type, amount, and concentration of disinfectants used in different hospital departments were other reasons for resistance to antiseptics in medical environments. Indiscriminate use of antibiotics caused microbial resistance in the same way antibiotics did not affect many microbial diseases, and arbitrary use caused the highest microbial resistance.","PeriodicalId":507382,"journal":{"name":"Journal of Kermanshah University of Medical Sciences","volume":"4 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141686201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Olarewaju, A. Sokan-Adeaga, Fasanmi Akinlolu, Olufunke Julianah Ogidan, Micheal Ayodeji Sokan-Adeaga, Joy Stephen Amusan
Background: Sawmilling exposed workers to diverse occupational mishaps and subsequent health complications. Investigating workplace risk and health status can help mitigate morbidness and death among this group. Objectives: This study aimed to evaluate the occupational hazards and health status of sawmill workers in Akure, Nigeria. Methods: This cross-sectional descriptive survey was conducted on 304 sawmill workers in Akure metropolis selected via multi-stage sampling methodology. Data were collected using a pre-tested, standardized questionnaire. In addition, the body mass index (BMI), blood pressure (mmHg), and respiratory function parameters (spirometry) were measured. Descriptive and inferential statistics (chi-square) were used to evaluate and summarize the data, with a significance level of P < 0.05. Results: The respondents' average age was 42.0 ± 2.8 years. The common reported workplace hazards included noise 273 (89.8%), manual lifting of objects 221 (72.7%), heat 192 (63.2%), and wood dust 192 (63.2%), while common reported injuries were bruises 292 (96.1%), hearing impairment 281 (92.4%), electric shock 250 (82.2%), and fainting attack 232 (76.3%). The common prevalent ailments suffered by respondents in the last month were cough 158 (52.0%), phlegm production 149 (49.0%), chest pain 63 (20.7%), skin problems 100 (32.9%), and eyes irritation 111 (36.5%). The anthropometric measurement showed that most 272 (89.5%) sawmill workers are overweight and obese. Spirometry measurements revealed that one-third of the respondents had a forced expiratory ratio (FER) measurement below 70.00% and an abnormal respiratory rate. The bivariate analysis showed a significant correlation between the respondents' self-reported prior workplace injury, knowledge of workplace dangers, and sociodemographic characteristics (job category and educational level). Conclusions: The results showed that the respondents suffered from impaired respiratory function related to workplace hazard exposure and the non-usage of protective devices during operational activities. In addition, the majority are at risk of developing diverse cardiovascular and respiratory diseases due to workplace exposures.
{"title":"Assessment of Occupational Hazards and Health Status","authors":"S. Olarewaju, A. Sokan-Adeaga, Fasanmi Akinlolu, Olufunke Julianah Ogidan, Micheal Ayodeji Sokan-Adeaga, Joy Stephen Amusan","doi":"10.5812/jkums-144054","DOIUrl":"https://doi.org/10.5812/jkums-144054","url":null,"abstract":"Background: Sawmilling exposed workers to diverse occupational mishaps and subsequent health complications. Investigating workplace risk and health status can help mitigate morbidness and death among this group. Objectives: This study aimed to evaluate the occupational hazards and health status of sawmill workers in Akure, Nigeria. Methods: This cross-sectional descriptive survey was conducted on 304 sawmill workers in Akure metropolis selected via multi-stage sampling methodology. Data were collected using a pre-tested, standardized questionnaire. In addition, the body mass index (BMI), blood pressure (mmHg), and respiratory function parameters (spirometry) were measured. Descriptive and inferential statistics (chi-square) were used to evaluate and summarize the data, with a significance level of P < 0.05. Results: The respondents' average age was 42.0 ± 2.8 years. The common reported workplace hazards included noise 273 (89.8%), manual lifting of objects 221 (72.7%), heat 192 (63.2%), and wood dust 192 (63.2%), while common reported injuries were bruises 292 (96.1%), hearing impairment 281 (92.4%), electric shock 250 (82.2%), and fainting attack 232 (76.3%). The common prevalent ailments suffered by respondents in the last month were cough 158 (52.0%), phlegm production 149 (49.0%), chest pain 63 (20.7%), skin problems 100 (32.9%), and eyes irritation 111 (36.5%). The anthropometric measurement showed that most 272 (89.5%) sawmill workers are overweight and obese. Spirometry measurements revealed that one-third of the respondents had a forced expiratory ratio (FER) measurement below 70.00% and an abnormal respiratory rate. The bivariate analysis showed a significant correlation between the respondents' self-reported prior workplace injury, knowledge of workplace dangers, and sociodemographic characteristics (job category and educational level). Conclusions: The results showed that the respondents suffered from impaired respiratory function related to workplace hazard exposure and the non-usage of protective devices during operational activities. In addition, the majority are at risk of developing diverse cardiovascular and respiratory diseases due to workplace exposures.","PeriodicalId":507382,"journal":{"name":"Journal of Kermanshah University of Medical Sciences","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141701004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sayed Nassereddin Mostafavi Esfahani, S. Rostami, Zahra Amini
Background: Pseudomonas aeruginosa is a ubiquitous bacillus responsible for severe infections in inpatients, especially nosocomial and intensive care unit (ICU) infections. Objectives: This study aimed to determine the antibiotic susceptibility of clinical isolates from inpatients in three referral hospitals in Isfahan, Iran. Methods: Standard tests identified the organism and antibiotic susceptibility testing. Stratification was performed by place of infection (community, hospital), admission ward (ICU, non-ICU), and age group (< 20 versus > 20 years). Results: P. aeruginosa showed high susceptibility to colistin (100%) and amikacin (81.8%) followed by tobramycin (69.2%), ciprofloxacin (68.5%), meropenem (67.2%), cefepime (65.7%), ceftazidime (64.3%), and imipenem (63.3%). Community-acquired strains were significantly more susceptible to meropenem (81.6%), ciprofloxacin (77.1%), cefepime (77.1%), imipenem (74.3%), and ceftazidime (72.2%) than nosocomial strains. Non-ICU isolates were more susceptible to carbapenems. P. aeruginosa isolates had higher antibiotic susceptibility in less than 20 years. Conclusions: Based on the results, a combination of colistin and amikacin would be appropriate for the empiric treatment of suspected P. aeruginosa infections in severe cases, nosocomial infections, or patients admitted to ICU. Ceftazidime, cefepime, ciprofloxacin, meropenem, or imipenem would be suitable for mild to moderate infections, especially in community-acquired infections.
{"title":"Antibiotic Susceptibility Pattern of Nosocomial and Community-Acquired Pseudomonas aeruginosa in Isfahan: A Prospective Multicenter Study","authors":"Sayed Nassereddin Mostafavi Esfahani, S. Rostami, Zahra Amini","doi":"10.5812/jkums-140315","DOIUrl":"https://doi.org/10.5812/jkums-140315","url":null,"abstract":"Background: Pseudomonas aeruginosa is a ubiquitous bacillus responsible for severe infections in inpatients, especially nosocomial and intensive care unit (ICU) infections. Objectives: This study aimed to determine the antibiotic susceptibility of clinical isolates from inpatients in three referral hospitals in Isfahan, Iran. Methods: Standard tests identified the organism and antibiotic susceptibility testing. Stratification was performed by place of infection (community, hospital), admission ward (ICU, non-ICU), and age group (< 20 versus > 20 years). Results: P. aeruginosa showed high susceptibility to colistin (100%) and amikacin (81.8%) followed by tobramycin (69.2%), ciprofloxacin (68.5%), meropenem (67.2%), cefepime (65.7%), ceftazidime (64.3%), and imipenem (63.3%). Community-acquired strains were significantly more susceptible to meropenem (81.6%), ciprofloxacin (77.1%), cefepime (77.1%), imipenem (74.3%), and ceftazidime (72.2%) than nosocomial strains. Non-ICU isolates were more susceptible to carbapenems. P. aeruginosa isolates had higher antibiotic susceptibility in less than 20 years. Conclusions: Based on the results, a combination of colistin and amikacin would be appropriate for the empiric treatment of suspected P. aeruginosa infections in severe cases, nosocomial infections, or patients admitted to ICU. Ceftazidime, cefepime, ciprofloxacin, meropenem, or imipenem would be suitable for mild to moderate infections, especially in community-acquired infections.","PeriodicalId":507382,"journal":{"name":"Journal of Kermanshah University of Medical Sciences","volume":"31 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140425006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Habibolah Khazaie, M. Rostampour, L. Rezaie, Saeid Komasi, A. Zakiei
Background: Sleep disorders are a public health problem, and sleep studies among ethnic groups are few. Therefore, this study aimed to evaluate sleep quality and sleep problems among Iranian people. Methods: This cross-sectional study was conducted on 4748 young people aged 18 to 35 (average: 27.58 years) in western Iran during the COVID-19 pandemic. In addition, 60.4% of the participants were men. The data were collected using the Pittsburgh Sleep Quality Inventory (PSQI), the Insomnia Severity Index (ISI), and the Demographic Information Questionnaire and analyzed with SPSS software version 22. Results: About 44.4% of the participants had unfavorable sleep quality and had sleep problems. Besides, the mean score of sleep problems (measured with PSQI) reported by the participants was 5.68±2.67. The logistic regression analysis showed that the participant’s age, education, marital status, occupation, drug abuse, alcohol consumption, use of mobile phones and the internet, and a history of previous sleep problems accounted for sleep problems during the COVID-19 pandemic. Conclusions: Based on the results, there was a high prevalence of sleep problems among Iranian young people during the COVID-19 pandemic.
{"title":"Factors Affecting Sleep Problems Among Young People During COVID-19 Pandemic: A Report from Western Iran","authors":"Habibolah Khazaie, M. Rostampour, L. Rezaie, Saeid Komasi, A. Zakiei","doi":"10.5812/jkums-141148","DOIUrl":"https://doi.org/10.5812/jkums-141148","url":null,"abstract":"Background: Sleep disorders are a public health problem, and sleep studies among ethnic groups are few. Therefore, this study aimed to evaluate sleep quality and sleep problems among Iranian people. Methods: This cross-sectional study was conducted on 4748 young people aged 18 to 35 (average: 27.58 years) in western Iran during the COVID-19 pandemic. In addition, 60.4% of the participants were men. The data were collected using the Pittsburgh Sleep Quality Inventory (PSQI), the Insomnia Severity Index (ISI), and the Demographic Information Questionnaire and analyzed with SPSS software version 22. Results: About 44.4% of the participants had unfavorable sleep quality and had sleep problems. Besides, the mean score of sleep problems (measured with PSQI) reported by the participants was 5.68±2.67. The logistic regression analysis showed that the participant’s age, education, marital status, occupation, drug abuse, alcohol consumption, use of mobile phones and the internet, and a history of previous sleep problems accounted for sleep problems during the COVID-19 pandemic. Conclusions: Based on the results, there was a high prevalence of sleep problems among Iranian young people during the COVID-19 pandemic.","PeriodicalId":507382,"journal":{"name":"Journal of Kermanshah University of Medical Sciences","volume":"8 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140430298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alireza Janbakhsh, Armin Naghipour, Zahra Naghibifar, Abbass Moghimbeigi, Marjan Balvandi
Background: Vaccination is one of the most cost-effective ways to prevent the spread of COVID-19. Objectives: The present study aimed to determine the prevalence of vaccine acceptance, side effects, and factors encouraging/disincentivizing vaccination among healthcare workers. Methods: A descriptive-analytical cross-sectional study was conducted on 315 health workers at Imam Reza Hospital, Kermanshah, Iran, in 2021. The samples were selected through a simple random sampling method. The data collection tool was a researcher-made questionnaire, and the collected data were analyzed in STATA-17 software. Results: The vaccine acceptance rate was 84.1% (n = 265), and the vaccine refusal rate was 15.9% (n = 50). The most injected vaccines were Sinopharm (n = 112, 42.3%) and Sputnik (n = 88, 33.2%), respectively. The most common side effects after vaccine injection were fatigue (34.8%, n=39) and myalgia (14.3%, n = 16). Being in a high-risk place (n = 199, 75.2%) and fear of contracting COVID-19 (n = 88, 33.5%) were the most important reasons for encouraging vaccination. About 36.7% (n = 18) of blood clotting, 26.5% (n = 14) cardiac arrest, short-term safety, and distrust were the essential factors for not accepting the vaccine. Conclusions: Based on the results, the prevalence of vaccine acceptance among health workers was high. However, a small percentage did not want to accept the vaccine due to negative news and a lack of trust in the vaccine. Therefore, it is necessary to establish strong educational and awareness-raising associations that transmit knowledge of the COVID-19 vaccine by focusing on false information.
{"title":"Evaluating the Motivation to Accept/Not Accept the COVID-19 Vaccination and its Related Factors in Health Care Workers: A Cross-Sectional Study","authors":"Alireza Janbakhsh, Armin Naghipour, Zahra Naghibifar, Abbass Moghimbeigi, Marjan Balvandi","doi":"10.5812/jkums-138880","DOIUrl":"https://doi.org/10.5812/jkums-138880","url":null,"abstract":"Background: Vaccination is one of the most cost-effective ways to prevent the spread of COVID-19. Objectives: The present study aimed to determine the prevalence of vaccine acceptance, side effects, and factors encouraging/disincentivizing vaccination among healthcare workers. Methods: A descriptive-analytical cross-sectional study was conducted on 315 health workers at Imam Reza Hospital, Kermanshah, Iran, in 2021. The samples were selected through a simple random sampling method. The data collection tool was a researcher-made questionnaire, and the collected data were analyzed in STATA-17 software. Results: The vaccine acceptance rate was 84.1% (n = 265), and the vaccine refusal rate was 15.9% (n = 50). The most injected vaccines were Sinopharm (n = 112, 42.3%) and Sputnik (n = 88, 33.2%), respectively. The most common side effects after vaccine injection were fatigue (34.8%, n=39) and myalgia (14.3%, n = 16). Being in a high-risk place (n = 199, 75.2%) and fear of contracting COVID-19 (n = 88, 33.5%) were the most important reasons for encouraging vaccination. About 36.7% (n = 18) of blood clotting, 26.5% (n = 14) cardiac arrest, short-term safety, and distrust were the essential factors for not accepting the vaccine. Conclusions: Based on the results, the prevalence of vaccine acceptance among health workers was high. However, a small percentage did not want to accept the vaccine due to negative news and a lack of trust in the vaccine. Therefore, it is necessary to establish strong educational and awareness-raising associations that transmit knowledge of the COVID-19 vaccine by focusing on false information.","PeriodicalId":507382,"journal":{"name":"Journal of Kermanshah University of Medical Sciences","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Escherichia coli O157:H7 is a significant pathogen responsible for causing diarrhea in humans. Oxadiazoles are known for their diverse range of biological activities. Objectives: This study aimed to assess the anti-E. coli O157:H7 effects of 1, 3, and 4-oxadiazole derivatives. Methods: Compounds containing an oxadiazole central core were synthesized anew. In vitro assays, including agar well diffusion, minimum inhibitory concentration, and minimum bactericidal concentration, were conducted. The molecular structures of the oxadiazole derivatives were optimized using the mm2 methodology with Chem3D v20.1.1.125 software. The ligand's inhibitory potential against the active sites of stx-1 and stx-2 was assessed using Autodock Vina software. The results were analyzed using Discovery Studio v16.1.0 software. Results: The findings indicated that compound C ((2E)-3-(3,4-difluorophenyl)-2-(5-(hydroxy(pyridin-2-yl)meth yl)-1,3,4-oxadiazol-2-yl)-N-methylacrylimidic acid) exhibited more potent anti-E. coli O157:H7 effects compared to other compounds and the control sample. Furthermore, in silico results demonstrated that compound C exhibited inhibitory effects against stx-1 and stx-2 by forming hydrogen bonds for inhibition. Conclusions: Compounds featuring a fluorophenyl structure with a 1, 3, and 4-oxadiazole core have the potential to serve as anti-E. coli O157:H7 agents for the development of therapeutic drugs.
{"title":"In vitro and in silico Evaluation of Antibacterial Effects in Some Oxadiazole Compounds against Escherichia coli O157: H7","authors":"Yasin SarveAhrabi, Sarina Nejati Khoei","doi":"10.5812/jkums-139825","DOIUrl":"https://doi.org/10.5812/jkums-139825","url":null,"abstract":"Background: Escherichia coli O157:H7 is a significant pathogen responsible for causing diarrhea in humans. Oxadiazoles are known for their diverse range of biological activities. Objectives: This study aimed to assess the anti-E. coli O157:H7 effects of 1, 3, and 4-oxadiazole derivatives. Methods: Compounds containing an oxadiazole central core were synthesized anew. In vitro assays, including agar well diffusion, minimum inhibitory concentration, and minimum bactericidal concentration, were conducted. The molecular structures of the oxadiazole derivatives were optimized using the mm2 methodology with Chem3D v20.1.1.125 software. The ligand's inhibitory potential against the active sites of stx-1 and stx-2 was assessed using Autodock Vina software. The results were analyzed using Discovery Studio v16.1.0 software. Results: The findings indicated that compound C ((2E)-3-(3,4-difluorophenyl)-2-(5-(hydroxy(pyridin-2-yl)meth yl)-1,3,4-oxadiazol-2-yl)-N-methylacrylimidic acid) exhibited more potent anti-E. coli O157:H7 effects compared to other compounds and the control sample. Furthermore, in silico results demonstrated that compound C exhibited inhibitory effects against stx-1 and stx-2 by forming hydrogen bonds for inhibition. Conclusions: Compounds featuring a fluorophenyl structure with a 1, 3, and 4-oxadiazole core have the potential to serve as anti-E. coli O157:H7 agents for the development of therapeutic drugs.","PeriodicalId":507382,"journal":{"name":"Journal of Kermanshah University of Medical Sciences","volume":"40 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140435043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fahimeh Edalat, Neda Pirbonyeh, J. Sarvari, Nasir Arefinia, M. Kadivar, Omid Rashidi, A. Moattari
Background: Variation in the hemagglutinin (HA) of influenza A/H3N2 can affect antigenicity and virulence, potentially leading to the emergence of vaccine escape variants and antigenic shift viruses. This study aimed to evaluate the genetic variation of the HA gene to enhance global influenza virus surveillance. Objectives: The primary objectives of this research included molecular characterization and phylogenetic analysis of the influenza A/H3N2 HA gene. Therefore, this research aimed to contribute valuable insights to influenza surveillance efforts on a global scale by identifying the variants associated with vaccine escape and antigenic shift. Methods: RNA extraction was performed on 300 nasopharyngeal swab samples from patients with influenza-like illness using a commercial kit. The presence of influenza A/H3N2 was determined using qRT-PCR. Subsequently, positive samples underwent HA gene amplification through RT-PCR using the Sanger method. Variability in HA immunological sites was compared with reference sequences, and genotype/subtype was determined. Alignment and phylogenetic analyses were conducted to construct a tree utilizing an optimal nucleotide substitution model Results: Among the 300 samples, 22 (7.33%) tested positive for influenza A/H3N2, with a male distribution of 13 cases. The mean age of the participants was 35 ± 2.40 years. The analysis of antigenic site mutations revealed significant variations (A, B, D, and E) in the HA gene, with major mutations at positions 140 and 186. Noteworthy mutations such as S159Y and the previously undocumented K83R substitution at antigenic site E. Phylogenetic analysis classified the A/H3N2 strain into clades 3C.2a and 3C.3. Conclusions: The identified mutations in the HA gene suggest potential changes in antigenicity, emphasizing their relevance to vaccine development and surveillance strategies. It is recommended that ongoing molecular investigations be conducted for effective influenza control; also, the ongoing need to monitor influenza viruses is suggested to be highlighted. This research provides valuable insights necessary for maintaining efficient influenza control measures globally.
背景：甲型 H3N2 流感病毒血凝素（HA）的变异会影响抗原性和毒力，可能导致疫苗逃逸变种和抗原转变病毒的出现。本研究旨在评估 HA 基因的遗传变异，以加强全球流感病毒监测。目标：本研究的主要目标包括甲型 H3N2 流感 HA 基因的分子特征和系统发育分析。因此，本研究旨在通过确定与疫苗逃逸和抗原转变相关的变异，为全球范围内的流感监测工作提供有价值的见解。研究方法使用商业试剂盒对 300 份流感样疾病患者的鼻咽拭子样本进行 RNA 提取。采用 qRT-PCR 方法确定是否存在 A/H3N2 流感病毒。随后，采用 Sanger 方法通过 RT-PCR 对阳性样本进行 HA 基因扩增。将 HA 免疫位点的变异性与参考序列进行比较，并确定基因型/亚型。结果：在 300 份样本中，22 人（7.33%）的甲型 H3N2 流感检测结果呈阳性，其中 13 例为男性。参与者的平均年龄为 35±2.40 岁。对抗原位点突变的分析表明，HA 基因有明显的变异（A、B、D 和 E），主要突变位于 140 和 186 位。值得注意的突变如 S159Y 和以前未记录的抗原位点 E 的 K83R 取代。系统发生学分析将 A/H3N2 株归入 3C.2a 和 3C.3 支系。结论已发现的 HA 基因突变表明抗原性可能会发生变化，强调了其与疫苗开发和监控策略的相关性。建议持续开展分子研究，以有效控制流感；还建议强调持续监测流感病毒的必要性。这项研究为全球保持有效的流感控制措施提供了宝贵的见解。
{"title":"Genetic Variation and Phylogenetic Analysis of Influenza A/H3N2 Hemagglutinin Gene in Shiraz, Southern Iran: Implications for Influenza Control and Vaccine Development","authors":"Fahimeh Edalat, Neda Pirbonyeh, J. Sarvari, Nasir Arefinia, M. Kadivar, Omid Rashidi, A. Moattari","doi":"10.5812/jkums-139001","DOIUrl":"https://doi.org/10.5812/jkums-139001","url":null,"abstract":"Background: Variation in the hemagglutinin (HA) of influenza A/H3N2 can affect antigenicity and virulence, potentially leading to the emergence of vaccine escape variants and antigenic shift viruses. This study aimed to evaluate the genetic variation of the HA gene to enhance global influenza virus surveillance. Objectives: The primary objectives of this research included molecular characterization and phylogenetic analysis of the influenza A/H3N2 HA gene. Therefore, this research aimed to contribute valuable insights to influenza surveillance efforts on a global scale by identifying the variants associated with vaccine escape and antigenic shift. Methods: RNA extraction was performed on 300 nasopharyngeal swab samples from patients with influenza-like illness using a commercial kit. The presence of influenza A/H3N2 was determined using qRT-PCR. Subsequently, positive samples underwent HA gene amplification through RT-PCR using the Sanger method. Variability in HA immunological sites was compared with reference sequences, and genotype/subtype was determined. Alignment and phylogenetic analyses were conducted to construct a tree utilizing an optimal nucleotide substitution model Results: Among the 300 samples, 22 (7.33%) tested positive for influenza A/H3N2, with a male distribution of 13 cases. The mean age of the participants was 35 ± 2.40 years. The analysis of antigenic site mutations revealed significant variations (A, B, D, and E) in the HA gene, with major mutations at positions 140 and 186. Noteworthy mutations such as S159Y and the previously undocumented K83R substitution at antigenic site E. Phylogenetic analysis classified the A/H3N2 strain into clades 3C.2a and 3C.3. Conclusions: The identified mutations in the HA gene suggest potential changes in antigenicity, emphasizing their relevance to vaccine development and surveillance strategies. It is recommended that ongoing molecular investigations be conducted for effective influenza control; also, the ongoing need to monitor influenza viruses is suggested to be highlighted. This research provides valuable insights necessary for maintaining efficient influenza control measures globally.","PeriodicalId":507382,"journal":{"name":"Journal of Kermanshah University of Medical Sciences","volume":"97 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140444140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MohammadHosein Zamanian, Zohreh Javadfar, Yahay Salimi, Mer Ali Rahimi, Elham Rabieenia, Akram Rahimi
Background: Evidence indicating the effect of vitamin D supplementation on improving the clinical outcomes of COVID-19 is limited. Objectives: This study aimed to examine the effect of high-dose vitamin D on mortality and hospital length of stay in ICU patients with COVID-19. Methods: A single-center, double-blind, placebo-controlled, randomized clinical trial study was conducted on 61 patients with moderate to severe COVID-19 hospitalized in the ICU of Golestan Hospital, Kermanshah, Iran. Participants were randomly divided to receive a single dose of 300 000 IU vitamin D3 intramuscularly (n = 31) or placebo (n = 30). The primary outcomes were the length of hospital stay and in-hospital mortality. Secondary outcomes were the length of stay in the ICU, the number of patients requiring mechanical ventilation, and the serum level of 25-hydroxyvitamin D. Results: Out of 61 randomized patients, 44 patients with a mean age of 53.49 ± 13.2 years, body mass index of 29.80 ± 5.0 kg/m2, and serum vitamin D level of 24.36 ± 4.47 mg/dL were included in the study for primary analysis, of whom 31 (70.5%) cases were men. Median (interquartile range), the length of hospital stay between the vitamin D group (5.0 [6.0 - 11.0] days), placebo group (11.0 [6.0 - 17.0] days) (log-rank P = 0.23; unadjusted hazard ratio [HR] for hospital discharge, 3.4 [95% confidence interval [CI], 0.4 - 28.05]; P = 0.25; adjusted HR, 0.04 [95%CI, 11.52 - 0.0001]; P = 0.27) and mortality (unadjusted odds ratio [OR], 0.63 [95% CI,0.09 - 4.21]; P = 0.63; adjusted OR, 0.46 [95%CI, 0.03 - 6.55]; P = 0.57) showed no significant difference between the two groups before and after the adjustment of confounding factors. No significant difference was observed between the two groups regarding the need for mechanical ventilation (unadjusted OR for intubation = 2.1 [95% CI, 0.17 - 25.01]; P = 0.55), adjusted OR = 1.16 [95%CI, 0.04 - 27.57]; P = 0.92), and the length of stay in ICUs (log-rank = 2.49, P = 0.114; unadjusted HR for ICU discharge, 6.28 [95%CI, 0.5 - 78.42]; P = 0.15; adjusted HR = 21.48 [95%CI, 0.12 - 3677.48]; P = 0.24). Conclusions: Based on the results, A high dose of vitamin D did not significantly reduce mortality and hospital length of stay in ICU patients with COVID-19.
背景：有关补充维生素 D 对改善 COVID-19 临床疗效的证据有限。研究目的本研究旨在探讨大剂量维生素 D 对 COVID-19 ICU 患者死亡率和住院时间的影响。研究方法在伊朗克尔曼沙阿市戈勒斯坦医院重症监护室住院的 61 名中重度 COVID-19 患者中开展了一项单中心、双盲、安慰剂对照、随机临床试验研究。参与者被随机分为肌肉注射单剂量 300 000 IU 维生素 D3（31 人）或安慰剂（30 人）。主要结果是住院时间和院内死亡率。次要结果是重症监护室的住院时间、需要机械通气的患者人数以及血清中 25- 羟维生素 D 的水平：在 61 名随机患者中，44 名患者的平均年龄为（53.49 ± 13.2）岁，体重指数为（29.80 ± 5.0）kg/m2，血清维生素 D 水平为（24.36 ± 4.47）mg/dL，其中 31 例（70.5%）为男性。维生素 D 组（5.0 [6.0 - 11.0] 天）、安慰剂组（11.0 [6.0 - 17.0] 天）的住院时间中位数（四分位数间距）（对数秩 P = 0.23；未调整出院危险比 [HR]，3.4 [95% 置信区间 [CI]，0.4 - 28.05]；P = 0.25；调整后 HR，0.04 [95%CI，11.52 - 0.0001]；P = 0.27）和死亡率（未调整的几率比[OR]，0.63 [95% CI，0.09 - 4.21]；P = 0.63；调整后的 OR，0.46 [95%CI，0.03 - 6.55]；P = 0.57）显示两组在调整混杂因素前后无显著差异。两组在机械通气需求方面无明显差异（未调整插管 OR = 2.1 [95%CI, 0.17 - 25.01]; P = 0.55），调整后 OR = 1.16 [95%CI, 0.04 - 27.57]; P = 0.92），以及在 ICU 的住院时间（对数秩 = 2.49，P = 0.114；ICU 出院的未调整 HR，6.28 [95%CI, 0.5 - 78.42]; P = 0.15；调整 HR = 21.48 [95%CI, 0.12 - 3677.48]; P = 0.24）。结论根据研究结果，大剂量维生素 D 并不能显著降低 COVID-19 ICU 患者的死亡率和住院时间。
{"title":"Effect of High-Dose Vitamin D on Mortality and Hospital Length of Stay in ICU Patients with COVID-19: A Randomized Clinical Trial","authors":"MohammadHosein Zamanian, Zohreh Javadfar, Yahay Salimi, Mer Ali Rahimi, Elham Rabieenia, Akram Rahimi","doi":"10.5812/jkums-140510","DOIUrl":"https://doi.org/10.5812/jkums-140510","url":null,"abstract":"Background: Evidence indicating the effect of vitamin D supplementation on improving the clinical outcomes of COVID-19 is limited. Objectives: This study aimed to examine the effect of high-dose vitamin D on mortality and hospital length of stay in ICU patients with COVID-19. Methods: A single-center, double-blind, placebo-controlled, randomized clinical trial study was conducted on 61 patients with moderate to severe COVID-19 hospitalized in the ICU of Golestan Hospital, Kermanshah, Iran. Participants were randomly divided to receive a single dose of 300 000 IU vitamin D3 intramuscularly (n = 31) or placebo (n = 30). The primary outcomes were the length of hospital stay and in-hospital mortality. Secondary outcomes were the length of stay in the ICU, the number of patients requiring mechanical ventilation, and the serum level of 25-hydroxyvitamin D. Results: Out of 61 randomized patients, 44 patients with a mean age of 53.49 ± 13.2 years, body mass index of 29.80 ± 5.0 kg/m2, and serum vitamin D level of 24.36 ± 4.47 mg/dL were included in the study for primary analysis, of whom 31 (70.5%) cases were men. Median (interquartile range), the length of hospital stay between the vitamin D group (5.0 [6.0 - 11.0] days), placebo group (11.0 [6.0 - 17.0] days) (log-rank P = 0.23; unadjusted hazard ratio [HR] for hospital discharge, 3.4 [95% confidence interval [CI], 0.4 - 28.05]; P = 0.25; adjusted HR, 0.04 [95%CI, 11.52 - 0.0001]; P = 0.27) and mortality (unadjusted odds ratio [OR], 0.63 [95% CI,0.09 - 4.21]; P = 0.63; adjusted OR, 0.46 [95%CI, 0.03 - 6.55]; P = 0.57) showed no significant difference between the two groups before and after the adjustment of confounding factors. No significant difference was observed between the two groups regarding the need for mechanical ventilation (unadjusted OR for intubation = 2.1 [95% CI, 0.17 - 25.01]; P = 0.55), adjusted OR = 1.16 [95%CI, 0.04 - 27.57]; P = 0.92), and the length of stay in ICUs (log-rank = 2.49, P = 0.114; unadjusted HR for ICU discharge, 6.28 [95%CI, 0.5 - 78.42]; P = 0.15; adjusted HR = 21.48 [95%CI, 0.12 - 3677.48]; P = 0.24). Conclusions: Based on the results, A high dose of vitamin D did not significantly reduce mortality and hospital length of stay in ICU patients with COVID-19.","PeriodicalId":507382,"journal":{"name":"Journal of Kermanshah University of Medical Sciences","volume":"75 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139836327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MohammadHosein Zamanian, Zohreh Javadfar, Yahay Salimi, Mer Ali Rahimi, Elham Rabieenia, Akram Rahimi
Background: Evidence indicating the effect of vitamin D supplementation on improving the clinical outcomes of COVID-19 is limited. Objectives: This study aimed to examine the effect of high-dose vitamin D on mortality and hospital length of stay in ICU patients with COVID-19. Methods: A single-center, double-blind, placebo-controlled, randomized clinical trial study was conducted on 61 patients with moderate to severe COVID-19 hospitalized in the ICU of Golestan Hospital, Kermanshah, Iran. Participants were randomly divided to receive a single dose of 300 000 IU vitamin D3 intramuscularly (n = 31) or placebo (n = 30). The primary outcomes were the length of hospital stay and in-hospital mortality. Secondary outcomes were the length of stay in the ICU, the number of patients requiring mechanical ventilation, and the serum level of 25-hydroxyvitamin D. Results: Out of 61 randomized patients, 44 patients with a mean age of 53.49 ± 13.2 years, body mass index of 29.80 ± 5.0 kg/m2, and serum vitamin D level of 24.36 ± 4.47 mg/dL were included in the study for primary analysis, of whom 31 (70.5%) cases were men. Median (interquartile range), the length of hospital stay between the vitamin D group (5.0 [6.0 - 11.0] days), placebo group (11.0 [6.0 - 17.0] days) (log-rank P = 0.23; unadjusted hazard ratio [HR] for hospital discharge, 3.4 [95% confidence interval [CI], 0.4 - 28.05]; P = 0.25; adjusted HR, 0.04 [95%CI, 11.52 - 0.0001]; P = 0.27) and mortality (unadjusted odds ratio [OR], 0.63 [95% CI,0.09 - 4.21]; P = 0.63; adjusted OR, 0.46 [95%CI, 0.03 - 6.55]; P = 0.57) showed no significant difference between the two groups before and after the adjustment of confounding factors. No significant difference was observed between the two groups regarding the need for mechanical ventilation (unadjusted OR for intubation = 2.1 [95% CI, 0.17 - 25.01]; P = 0.55), adjusted OR = 1.16 [95%CI, 0.04 - 27.57]; P = 0.92), and the length of stay in ICUs (log-rank = 2.49, P = 0.114; unadjusted HR for ICU discharge, 6.28 [95%CI, 0.5 - 78.42]; P = 0.15; adjusted HR = 21.48 [95%CI, 0.12 - 3677.48]; P = 0.24). Conclusions: Based on the results, A high dose of vitamin D did not significantly reduce mortality and hospital length of stay in ICU patients with COVID-19.
背景：有关补充维生素 D 对改善 COVID-19 临床疗效的证据有限。研究目的本研究旨在探讨大剂量维生素 D 对 COVID-19 ICU 患者死亡率和住院时间的影响。研究方法在伊朗克尔曼沙阿市戈勒斯坦医院重症监护室住院的 61 名中重度 COVID-19 患者中开展了一项单中心、双盲、安慰剂对照、随机临床试验研究。参与者被随机分为肌肉注射单剂量 300 000 IU 维生素 D3（31 人）或安慰剂（30 人）。主要结果是住院时间和院内死亡率。次要结果是重症监护室的住院时间、需要机械通气的患者人数以及血清中 25- 羟维生素 D 的水平：在 61 名随机患者中，44 名患者的平均年龄为（53.49 ± 13.2）岁，体重指数为（29.80 ± 5.0）kg/m2，血清维生素 D 水平为（24.36 ± 4.47）mg/dL，其中 31 例（70.5%）为男性。维生素 D 组（5.0 [6.0 - 11.0] 天）、安慰剂组（11.0 [6.0 - 17.0] 天）的住院时间中位数（四分位数间距）（对数秩 P = 0.23；未调整出院危险比 [HR]，3.4 [95% 置信区间 [CI]，0.4 - 28.05]；P = 0.25；调整后 HR，0.04 [95%CI，11.52 - 0.0001]；P = 0.27）和死亡率（未调整的几率比[OR]，0.63 [95% CI，0.09 - 4.21]；P = 0.63；调整后的 OR，0.46 [95%CI，0.03 - 6.55]；P = 0.57）显示两组在调整混杂因素前后无显著差异。两组在机械通气需求方面无明显差异（未调整插管 OR = 2.1 [95%CI, 0.17 - 25.01]; P = 0.55），调整后 OR = 1.16 [95%CI, 0.04 - 27.57]; P = 0.92），以及在 ICU 的住院时间（对数秩 = 2.49，P = 0.114；ICU 出院的未调整 HR，6.28 [95%CI, 0.5 - 78.42]; P = 0.15；调整 HR = 21.48 [95%CI, 0.12 - 3677.48]; P = 0.24）。结论根据研究结果，大剂量维生素 D 并不能显著降低 COVID-19 ICU 患者的死亡率和住院时间。
{"title":"Effect of High-Dose Vitamin D on Mortality and Hospital Length of Stay in ICU Patients with COVID-19: A Randomized Clinical Trial","authors":"MohammadHosein Zamanian, Zohreh Javadfar, Yahay Salimi, Mer Ali Rahimi, Elham Rabieenia, Akram Rahimi","doi":"10.5812/jkums-140510","DOIUrl":"https://doi.org/10.5812/jkums-140510","url":null,"abstract":"Background: Evidence indicating the effect of vitamin D supplementation on improving the clinical outcomes of COVID-19 is limited. Objectives: This study aimed to examine the effect of high-dose vitamin D on mortality and hospital length of stay in ICU patients with COVID-19. Methods: A single-center, double-blind, placebo-controlled, randomized clinical trial study was conducted on 61 patients with moderate to severe COVID-19 hospitalized in the ICU of Golestan Hospital, Kermanshah, Iran. Participants were randomly divided to receive a single dose of 300 000 IU vitamin D3 intramuscularly (n = 31) or placebo (n = 30). The primary outcomes were the length of hospital stay and in-hospital mortality. Secondary outcomes were the length of stay in the ICU, the number of patients requiring mechanical ventilation, and the serum level of 25-hydroxyvitamin D. Results: Out of 61 randomized patients, 44 patients with a mean age of 53.49 ± 13.2 years, body mass index of 29.80 ± 5.0 kg/m2, and serum vitamin D level of 24.36 ± 4.47 mg/dL were included in the study for primary analysis, of whom 31 (70.5%) cases were men. Median (interquartile range), the length of hospital stay between the vitamin D group (5.0 [6.0 - 11.0] days), placebo group (11.0 [6.0 - 17.0] days) (log-rank P = 0.23; unadjusted hazard ratio [HR] for hospital discharge, 3.4 [95% confidence interval [CI], 0.4 - 28.05]; P = 0.25; adjusted HR, 0.04 [95%CI, 11.52 - 0.0001]; P = 0.27) and mortality (unadjusted odds ratio [OR], 0.63 [95% CI,0.09 - 4.21]; P = 0.63; adjusted OR, 0.46 [95%CI, 0.03 - 6.55]; P = 0.57) showed no significant difference between the two groups before and after the adjustment of confounding factors. No significant difference was observed between the two groups regarding the need for mechanical ventilation (unadjusted OR for intubation = 2.1 [95% CI, 0.17 - 25.01]; P = 0.55), adjusted OR = 1.16 [95%CI, 0.04 - 27.57]; P = 0.92), and the length of stay in ICUs (log-rank = 2.49, P = 0.114; unadjusted HR for ICU discharge, 6.28 [95%CI, 0.5 - 78.42]; P = 0.15; adjusted HR = 21.48 [95%CI, 0.12 - 3677.48]; P = 0.24). Conclusions: Based on the results, A high dose of vitamin D did not significantly reduce mortality and hospital length of stay in ICU patients with COVID-19.","PeriodicalId":507382,"journal":{"name":"Journal of Kermanshah University of Medical Sciences","volume":"19 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139776566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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