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For most of its history, biospeleology has been a poor cousin of the other speleological disciplines 在其历史的大部分时间里,生物洞穴学一直是其他洞穴学学科的穷亲戚
IF 0.7 4区 地球科学 Q4 GEOSCIENCES, MULTIDISCIPLINARY Pub Date : 2020-12-18 DOI: 10.3986/ac.v49i2-3.9391
Ivo Lučić
Our series of interviews with leading karstologists now turns to cave biologist. David Culver, an emeritus professor of environmental science at the American University (Washington DC, USA), talks about his relationship to karst and what subterranean biology has given to the geoscientific disciplines and what it has taken from them. His science approach is well reflected in the statement: “In the last few decades, I have done valuable little completely independent research, and collaboration with people with different skill groups has been critical”.
我们对著名岩溶学家的一系列采访现在转向洞穴生物学家。美国大学(美国华盛顿特区)环境科学名誉教授David Culver谈到了他与岩溶的关系,以及地下生物学给地球科学学科带来了什么,以及它从中获得了什么。他的科学方法很好地反映在声明中:“在过去的几十年里,我几乎没有做过有价值的完全独立的研究,与不同技能群体的人合作至关重要”。
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引用次数: 0
A voyage across limestone landscapes into the subterranean realm of caves 穿越石灰岩景观进入地下洞穴的旅程
IF 0.7 4区 地球科学 Q4 GEOSCIENCES, MULTIDISCIPLINARY Pub Date : 2020-12-18 DOI: 10.3986/ac.v49i2-3.9395
Peter Gedei
Only few  cave photography books chave reached a wide international recognition.  The best example is the book on Lechugilla Cave, published by  Speleo Projects in 1991. The book has been an inspiration to many cave photographers. Incredible photos from the »most beautifull cave in the world« were shot by two teams, led by  Urs Widmer Kevin Downey and Sure Ballman. The book also inspired Max Wisshak, one of the most recognised cave photographers, who dedicated his second book to the late photographer and publisher Urs Widmer.
只有少数几本洞穴摄影书籍获得了广泛的国际认可。最好的例子是Speleo Projects于1991年出版的关于Lechugilla洞穴的书。这本书启发了许多洞穴摄影师。来自“世界上最美丽的洞穴”的令人难以置信的照片是由Urs Widmer Kevin Downey和Sure Ballman带领的两个团队拍摄的。这本书也启发了最受认可的洞穴摄影师之一Max Wisshak,他将自己的第二本书献给了已故摄影师兼出版商Urs Widmer。
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引用次数: 0
Fate of Contaminants of Emerging Concern in a Sinkhole Lake, Florida, USA 美国佛罗里达州沉孔湖中新出现的污染物的命运
IF 0.7 4区 地球科学 Q4 GEOSCIENCES, MULTIDISCIPLINARY Pub Date : 2020-12-16 DOI: 10.3986/ac.v49i2-3.8007
Van Beynen Philip, E. Upton, Ela Bialkowska-Jelinska, Laurent Calcul
Highly karstified carbonate platforms such as Florida are characterized by rapid infiltration rates, highly permeable bedrock and the direct connection to the below aquifer through the high density of sinkholes. This combination of physical features makes the groundwater and aquifers highly vulnerable to contamination from synthetic chemicals commonly referred to as contaminants of emerging concern (CECs). The use of septic tanks, otherwise referred to as onsite water treatment systems (OWTS), promotes the introduction of CECs into the environment. In order to study the impacts of CECs from OWTS on a karst landscape, water, sediment, and vegetation samples were collected in a sinkhole lake surrounded by residential housing using this waste disposal method. The main question of this research project is what is the fate of CECs from OWTSs effluent within the catchment of a sinkhole lake? Liquid chromatograph mass spectrometry was used to analyze the samples for the presence of CECs. It was found that the relative quantity of CECs in the individual constituents is dependent upon 1) the hydrophobicity and polarity of the individual compound, 2) the specific sampling site, 3) the topography gradient, and 4) for vegetation, the connectedness of the sample type to the sediment. Hydrogeological studies have found that the sinkholes of the area are all connected to the below aquifer. Consequently, these CECs pose a risk of the contamination of the groundwater. This study is a temporal snapshot, that being the dry season of Florida which is most likely the time of lowest CEC contamination. It is imperative that sampling extend into the wet season when flushing of CECs from the OWTS may increase their concentrations in both the lake but also the aquifers especially since residents use well water as their source of potable water. While this study is based in Florida, we strongly suspect that our findings and recommendations are applicable more generally as OWTS are used throughout the many karst regions of the world.
佛罗里达等高度岩溶的碳酸盐岩平台具有快速渗透率、高渗透性基岩以及通过高密度的天坑与地下含水层直接连接的特点。这种物理特征的结合使地下水和含水层极易受到合成化学物质的污染,通常被称为新出现的污染物(CECs)。化粪池的使用,也被称为现场水处理系统(OWTS),促进了CEC进入环境。为了研究OWTS的CEC对岩溶景观的影响,使用这种废物处理方法在住宅周围的天坑湖中收集了水、沉积物和植被样本。这个研究项目的主要问题是,在天坑湖的集水区内,来自OWTS废水的CEC的命运是什么?液相色谱-质谱法用于分析样品中CECs的存在。研究发现,CECs在单个成分中的相对数量取决于1)单个化合物的疏水性和极性,2)特定的采样点,3)地形梯度,以及4)对于植被,样品类型与沉积物的连通性。水文地质研究发现,该地区的天坑都与下面的含水层相连。因此,这些CEC存在地下水污染的风险。这项研究是一个时间快照,佛罗里达州的旱季很可能是CEC污染最低的时候。采样必须延伸到雨季,因为从OWTS冲洗CEC可能会增加其在湖泊和含水层中的浓度,特别是因为居民使用井水作为饮用水源。虽然这项研究是在佛罗里达州进行的,但我们强烈怀疑,由于OWTS在世界上许多岩溶地区都有使用,我们的发现和建议更普遍地适用。
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引用次数: 2
The microbial community structure of the Dupnisa cave in Kırklareli, Turkey 土耳其Kırklareli的Dupnisa洞穴的微生物群落结构
IF 0.7 4区 地球科学 Q4 GEOSCIENCES, MULTIDISCIPLINARY Pub Date : 2020-12-16 DOI: 10.3986/ac.v49i2-3.8575
Nihal Doğruöz-Güngör
Cave ecosystems count to be extreme environments due to their stable temperature, darkness, high humidity rates, and limited organic materials. In this context, these ecosystems represent invaluable laboratories for microbiological studies. Although there are common features between the microorganism groups obtained from the culture-based microbiological studies conducted in the caves and the groups highlighted through molecular methods, the microorganism groups determined through this last method are richer. The detected microorganisms are variable depending on the characteristics of each cave. The aim of this study is to determine the microbial diversity in samples taken from 5 different regions (including regions visited by tourists) of Dupnisa Cave and to reveal the differences between these regions. This is the first microbiological study running in cave sediments of Dupnisa Cave System situated in the north-western of Turkey. In this study, using the Illumina MiSeq next-generation sequencing approach for analyses of Dupnisa Cave samples, 14 phyla and 298 genera as well as 2 phyla and 20 genera can be attributed to bacterial and archaea OTUs, respectively. Moreover, the bacterial community is dominated by the phyla Proteobacteria, Actinobacteria, Bacteroidetes, Gemmatimonadetes, Firmicutes, Nitrospirae, Chloroflexi and Acidobacteria distributed with 1 % and above. Archaeal community is represented by Thaumarchaeota and Euryarchaeota phyla. Proteobacteria is the most dominant bacterial phylum and Thaumarchaeota dominates the archaeal phyla. The highest number of types of bacteria according to Chao 1 richness estimation index were found at point AF (cave entrance / sediment), and that of types of archaea were found at point F2 (touristic area 2 / cave sediment). F2 was determined as the sampling point with the highest diversity of archaeal and bacterial genera according to Shannon-Wiener diversity index.
洞穴生态系统由于其稳定的温度、黑暗、高湿度和有限的有机物质而被视为极端环境。在这种情况下,这些生态系统代表了微生物研究的宝贵实验室。尽管从洞穴中进行的基于培养的微生物研究中获得的微生物群与通过分子方法突出的微生物群之间存在共同特征,但通过最后一种方法确定的微生物群更丰富。根据每个洞穴的特征,检测到的微生物是可变的。本研究的目的是确定从杜普尼萨洞穴的5个不同地区(包括游客到访的地区)采集的样本中的微生物多样性,并揭示这些地区之间的差异。这是首次在土耳其西北部Dupnisa洞穴系统的洞穴沉积物中进行微生物学研究。在本研究中,使用Illumina MiSeq下一代测序方法分析Dupnisa洞穴样本,可以分别将14个门和298个属以及2个门和20个属归属于细菌和古菌OTU。此外,细菌群落以变形菌门、放线菌门、拟杆菌门、双球菌门、厚壁菌门、硝化螺旋菌门、氯弯菌门和酸杆菌门为主,分布在1%及以上。古生物群落以Thaumarchaeota和Euryarchaeota门为代表。变形菌门是最具优势的细菌门,Thaumarchaeota在古菌门中占主导地位。根据Chao 1丰富度估计指数,细菌类型最多的是AF点(洞穴入口/沉积物),而古菌类型最多的则是F2点(旅游区2/洞穴沉积物)。根据Shannon Wiener多样性指数,F2被确定为古菌属和细菌属多样性最高的采样点。
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引用次数: 3
Antimicrobial resistant Escherichia coli from karst waters, surfaces and bat guano in Slovenian caves 斯洛文尼亚洞穴中岩溶水、地表和蝙蝠粪中的抗微生物大肠杆菌
IF 0.7 4区 地球科学 Q4 GEOSCIENCES, MULTIDISCIPLINARY Pub Date : 2020-12-16 DOI: 10.3986/ac.v49i2-3.9103
J. Mulec, Blaž Kogovšek, S. Skok, R. Tomazin, S. Šturm, J. Avguštin
Escherichia coli, one of the primary intestinal commensal bacteria in humans and endothermic animals, is commonly considered an indicator of faecal pollution. E. coli strains were isolated from karst rivers under different hydrological conditions, from footpaths in tourist caves and from bat guano. Isolates were tested for phenotypic resistance to ampicillin, chloramphenicol, ciprofloxacin, nalidixic acid, tetracycline and trimethoprim. The highest percentage of antimicrobial resistant E. coli was found in karst waters, followed by those from surface swabs and from bat guano. Several isolates from rivers and swabs exhibited multidrug-resistant phenotype. Environmental conditions impact the populations of E. coli; a positive correlation between dissolved oxygen and E. coli counts, and a negative correlation between conductivity and E. coli concentrations have been observed for karst rivers. Malenščica (Slovenia), a drinking water resource with an extensive catchment area, contained a relative high percentage of antimicrobial-resistant E. coli strains. None of the isolates from bat guano was resistant to ampicillin, chloramphenicol, and tetracycline. Future monitoring of bats should consider a regular follow-up of indicative microbial disease indicators in fresh guano. Regular cleansing of tourist footpaths in caves and disinfection barriers at the cave entrances reduce the concentration and transmission of E. coli significantly. A future, more detailed, study on characterization of additional E. coli isolates is needed to reveal their pathogeneicity, mechanisms of antibiotic resistance, mobile genetic elements, and gene transfer frequencies to other members of the karst microbiome.
大肠杆菌是人类和吸热动物的主要肠道共生细菌之一,通常被认为是粪便污染的指标。从不同水文条件下的岩溶河流、旅游洞穴的步道和蝙蝠粪中分离到大肠杆菌菌株。分离株对氨苄青霉素、氯霉素、环丙沙星、萘啶酸、四环素和甲氧苄啶进行表型抗性测试。在岩溶水中发现的抗微生物大肠杆菌比例最高,其次是来自表面拭子和蝙蝠粪便的大肠杆菌。来自河流和拭子的几个分离株表现出多重耐药性表型。环境条件影响大肠杆菌种群;岩溶河流的溶解氧与大肠杆菌计数呈正相关,电导率与大肠杆菌浓度呈负相关。Malenščica(斯洛文尼亚)是一个拥有广泛集水区的饮用水资源,含有相对较高比例的抗微生物大肠杆菌菌株。蝙蝠粪中没有一个分离株对氨苄青霉素、氯霉素和四环素具有耐药性。未来对蝙蝠的监测应考虑定期跟踪新鲜鸟粪中的指示性微生物疾病指标。定期清洁洞穴内的旅游步道和洞穴入口处的消毒屏障,可显著降低大肠杆菌的浓度和传播。未来需要对其他大肠杆菌分离株的特征进行更详细的研究,以揭示它们的致病性、抗生素耐药性机制、可移动遗传元件以及向岩溶微生物组其他成员的基因转移频率。
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引用次数: 4
Assessment of karst geomorphosites on Kučaj and Beljanica mountains as a resource for the development of karst-based geopark ku<s:1> aj山和Beljanica山喀斯特地貌资源对喀斯特地质公园开发的价值评价
IF 0.7 4区 地球科学 Q4 GEOSCIENCES, MULTIDISCIPLINARY Pub Date : 2020-12-16 DOI: 10.3986/ac.v49i2-3.8748
A. Petrović, D. Nikolic, Dušica Trnavac Bogdanović, I. Carevic
Protection and promotion of geoheritage has been changing and improving in recent decades, in line with the growing research in this field. Some research papers specifically study geomorphological geoheritage and introduce a special term − geomorphosite. Karst geomorphosites present specific objects of geomorphological heritage. On Kučaj and Beljanica mountains there is the largest karst area in Serbia. The purpose of this paper is to select and evaluate the scientific value of karst geomorphosites of Kučaj and Beljanica. More than one hundred karst geomorphosites were analyzed. In order to achieve more adequate coverage and representation, they were grouped into 25 geomorphotypes in three major geomorphological components of the investigated area. The results of this analysis highlighted the most important natural potentials of this area for establishing a karst-based geopark. More than 20 geomorphotypes have a positive recommendation index, which provides a good basis for further geotourism analysis.
近几十年来,随着这一领域的研究日益增多,地质遗产的保护和推广也在不断变化和完善。一些研究论文专门研究了地貌地质遗产,并引入了一个专门的术语——地貌遗址。喀斯特地貌是地貌遗产的特殊对象。在ku aj和Beljanica山脉上有塞尔维亚最大的喀斯特地区。本文的目的是对ku aj和Beljanica喀斯特地貌的科学价值进行选择和评价。对一百多个岩溶地貌进行了分析。为了获得更充分的覆盖率和代表性,他们在调查地区的三个主要地貌组成部分分为25个地貌类型。分析结果突出了该地区建立岩溶型地质公园最重要的自然潜力。20多种地貌类型具有正向推荐指数,为进一步的地质旅游分析提供了良好的基础。
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引用次数: 3
Investigation of karst collapses using integrated geophysical methods: an example from Conghua district, Guangzhou city, China 综合地球物理方法研究岩溶塌陷——以广州市从化区为例
IF 0.7 4区 地球科学 Q4 GEOSCIENCES, MULTIDISCIPLINARY Pub Date : 2020-12-16 DOI: 10.3986/ac.v49i2-3.7786
Du Chengliang, Yongsheng Zhou, F. Gan, Yinxiang Chen
Integrated geophysical methods were used to investigate the geological conditions of karst collapses in Aotou, Conghua District, Guangzhou City. Based on audio frequency magnetotelluric (AMT) soundings, high-density electric signals and microtremors, a series of features, including the soil structure and thickness, faults, and hidden karst, were detected from shallow to deep subsurface regions in three-dimensional (3-D) space. These were then compared with geologic and drilling data. The measurements of micro-vibrations and high-density electric signals revealed that the strata in the collapsed area consists of clay and medium-grain sand, which is approximately 8–15 m thick and arranged as a multiple-element structure. The AMT soundings uncovered a hidden structure in the subsurface, which lies at the core of a syncline and adjacent to a contact zone between soluble limestone and non-soluble granite. Combined with the geologic data, the survey using the high-density electric method also showed that the study area hosts the Shidengzi Formation from the Datang Stage of the lower Carboniferous, which contain carbonaceous micrite and dolomitic limestone, locally intercalated with thin quartz siltstone, with well-developed karst. Collectively, the soil structure, hidden structure in the subsurface, and karst development provide the primary conditions for collapses. The main triggering factors for the karst collapses are seasonal variations in groundwater levels and excessive groundwater withdrawals. These results can provide guidance for the selection and application of geophysical methods for the examination of this issue in other areas with similar geology.
采用综合地球物理方法对广州市从化区坳头岩溶塌陷的地质条件进行了研究。基于音频大地电磁(AMT)测深、高密度电信号和微震,在三维空间中从浅层到深层探测到土壤结构与厚度、断层、隐伏岩溶等一系列特征。然后将这些数据与地质和钻井数据进行比较。微振动和高密度电信号测量表明,崩塌区地层由粘土和中粒砂组成,厚度约为8 ~ 15 m,呈多元结构。AMT探测在地下发现了一个隐藏的结构,它位于向斜的核心,靠近可溶石灰岩和不可溶花岗岩之间的接触带。结合地质资料,高密度电法测量还表明,研究区发育下石炭统大唐期石灯子组,含碳质泥晶岩和白云质灰岩,局部穿插薄石英粉砂岩,岩溶发育。土壤结构、地下隐伏结构和岩溶发育为塌陷提供了首要条件。岩溶塌陷的主要触发因素是地下水位的季节变化和地下水的过度开采。这些结果可为其他地质条件相似的地区研究该问题的地球物理方法的选择和应用提供指导。
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引用次数: 4
High resolution digital 3D modelling of subsurface morphological structures of Koutouki Cave, Greece 希腊Koutouki洞穴地下形态结构的高分辨率数字三维建模
IF 0.7 4区 地球科学 Q4 GEOSCIENCES, MULTIDISCIPLINARY Pub Date : 2020-12-16 DOI: 10.3986/ac.v49i2-3.7708
A. Konsolaki, E. Vassilakis, L. Gouliotis, Georgios Kontostavlos, Vassilis Giannopoulos
Remote sensing techniques and laser scanning technology have given us the opportunity to study indoor environments, such as caves, with their complex and unique morphology. In the presented case study, we used a handheld laser scanner for acquiring points with projected coordinate information (X, Y, Z) covering the entire show cave of Koutouki; including its hidden passages and dark corners. The point cloud covers the floor, the walls, and the roof of the cave, as well as the stalactites, stalagmites and the connected columns that constitute the decoration of the cave. The absolute and exact placement of the point cloud within a geographic reference frame gives us the opportunity for three-dimensional measurements and detailed visualization of the subsurface structures. Using open - source software, we managed to make a quantification analysis of the terrain and generated morphological and geometric features of the speleothems. We identified 55 columns by using digital terrain analysis and processed them statistically in order to correlate them to the frame of the cave development. The parameters that derived are the contours, each column height, the speleothem geometry and volume, as well as the volume of the open space cavity. We argue that by the demonstrated methodology, it is possible to identify with high accuracy and detail: the geomorphological features of a cave, an estimate of the speleogenesis, and the ability to monitor the evolution of a karstic system.Key words: cave, laser scanner, 3D representation, speleothems, SLAM.  
遥感技术和激光扫描技术使我们有机会研究室内环境,如洞穴,其复杂而独特的形态。在所提出的案例研究中,我们使用手持式激光扫描仪采集具有投影坐标信息(X,Y,Z)的点,该信息覆盖了Koutouki的整个表演洞穴;包括隐藏的通道和黑暗的角落。点云覆盖了洞穴的地面、墙壁和屋顶,以及构成洞穴装饰的钟乳石、石笋和相连的柱子。点云在地理参考系内的绝对和精确位置为我们提供了三维测量和地下结构详细可视化的机会。使用开源软件,我们设法对地形进行了量化分析,并生成了洞穴主题的形态和几何特征。我们通过使用数字地形分析确定了55个立柱,并对其进行了统计处理,以便将其与洞穴发育的框架相关联。导出的参数是轮廓、每个柱的高度、洞穴的几何形状和体积,以及开放空间洞穴的体积。我们认为,通过所证明的方法,可以高精度和详细地识别洞穴的地貌特征、洞穴成因的估计以及监测岩溶系统演化的能力。关键词:洞穴,激光扫描仪,三维表示,洞穴主题,SLAM。
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引用次数: 3
Information system for scientific study of the micrometeorology of karst caves – Case of Postojnska jama cave, Slovenija 用于溶洞微气象科学研究的信息系统——以斯洛文尼亚Postojinska jama洞穴为例
IF 0.7 4区 地球科学 Q4 GEOSCIENCES, MULTIDISCIPLINARY Pub Date : 2020-12-16 DOI: 10.3986/ac.v49i2-3.7540
P. Mlakar, B. Grasic, M. Božnar, Darko Popović, F. Gabrovšek
The paper presents the construction and operation of a cave information system on the example of the Postojnska jama cave. It thoroughly describes the construction of the basic modules of the cave information system, such as an automatic cave measuring station with sensors for measuring air temperature, air flow and CO2 concentration, and a central unit for collecting, saving, processing and displaying data. The system provides quality automatic measurements for the scientific study of the micrometeorology of karst caves. Its construction is based on good practices stemming from many years of experience in taking measurements in the external atmosphere and in karst caves. The paper is rich and comprehensive guideline for automatic cave measuring system construction for scientific and tourist management purposes.
本文以Postojnska jama洞穴为例,介绍了洞穴信息系统的建设和运行情况。详细介绍了溶洞信息系统基本模块的构建,包括具有温度、气流和CO2浓度传感器的溶洞自动测量站,以及数据采集、存储、处理和显示的中心单元。该系统为溶洞微气象学的科学研究提供了高质量的自动测量。它的建造是基于多年来在外部大气和溶洞中进行测量的良好经验。本文为洞穴自动测量系统的建设提供了丰富而全面的指导,以供科学和旅游管理之用。
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引用次数: 2
DNA, spectroscopic and geochemical analyses of bone fragments and associated speleothems in Postojna cave, Slovenia 斯洛文尼亚Postojna洞穴骨骼碎片和相关洞穴化石的DNA、光谱和地球化学分析
IF 0.7 4区 地球科学 Q4 GEOSCIENCES, MULTIDISCIPLINARY Pub Date : 2020-12-16 DOI: 10.3986/ac.v49i2-3.7970
M. Lipar, I. Z. Pajnič, M. Cotman, J. Z. Piano, Jian-xin Zgao, D. Pekarovič, T. Leskovar
a loose/broken stalagmite containing small fragments of cemented bones were collected from the Postojna Cave to investigate whether deoxyribonucleic acid (DNA) can be determined. The study is complemented by the Fourier-transform infrared spectroscopy - attenuated total reflectance (FTIR-ATR) analysis in order to determine the alteration of the bones and to test whether this analysis can be used as an indicator of possible DNA preservation. In addition, geochemical analyses were conducted in order to determine whether the associated flowstone/stalagmite is suitable for elucidating the timing of bone thanatocoenosis and further palaeoenvironmental analyses. The organic matter (collagen) is poorly preserved. However, we succeeded in amplifying a 94 bp long fragment of the cytochrome b (Cyt b) gene of mitochondrial DNA (mtDNA) in polymerase chain reaction (PCR) for one sample, and in sequencing the amto that of the Cyt b of the cave bear (Ursus deningeri or Ursus spelaeus sensu lato). The uranium-thorium dating of the speleothem covering the bones revealed its thanatocoenosis occurred prior to 55 ka, most likely in the late marine isotope stage 4 or early marine isotope stage 3. High porosity and recrystallisation of the flowstone/stalagmite at this part of the cave prevent high-resolution palaeoclimatic interpretation; however, low-resolution stable isotope geochemistry suggests a steppe-like environment during the subsequent growth of the speleothem.
从Postojna洞穴中收集了一个松散/破碎的石笋,其中含有小块胶结骨碎片,以研究是否可以确定脱氧核糖核酸(DNA)。该研究由傅里叶变换红外光谱-衰减全反射(FTIR-ATR)分析补充,以确定骨骼的变化,并测试该分析是否可以用作可能的DNA保存的指标。此外,还进行了地球化学分析,以确定相关的流石/石笋是否适合阐明骨死亡的时间和进一步的古环境分析。有机物(胶原蛋白)保存得很差。然而,我们成功地用聚合酶链反应(PCR)扩增了一个样本线粒体DNA (mtDNA)的细胞色素b (Cyt b)基因的94bp长片段,并对洞熊(Ursus deningeri或Ursus spelaeus sensu lato)的Cyt b进行了测序。对覆盖骨骼的岩石层进行铀钍定年,发现其死亡时间早于55 ka,极有可能发生在海洋同位素4期晚期或海洋同位素3期早期。洞内这部分流石/石笋的高孔隙度和重结晶影响了高分辨率的古气候解释;然而,低分辨率稳定同位素地球化学表明,在洞穴层随后的生长过程中,存在类似草原的环境。
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引用次数: 1
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