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In-vitro screening of amylase producing halophilic bacteria isolated from seawater 海水中产淀粉酶嗜盐菌的体外筛选
IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Pub Date : 2022-05-01 DOI: 10.56042/ijms.v51i05.65568
S. Abirami, M. Lakshmi, A. Samrot, P. J. J. Cypriyana, S. Saigeetha, G. Narendrakumar
In this study, eight halobacterial strains were isolated from seawater. Among the eight isolates, four strains grew well in nutrient medium with 40 g/100 mL of sodium chloride concentration. Amongst all, HA01 was found to show highest amylase activity than others. Highest amylase producing bacteria, i.e . HA01 was subjected for 16S rRNA sequence based identification and it was found to be Aquabacterium sp. strain AS02. The organism showed optimal amylase production at pH 7 and temperature of 42 °C and NaCl concentration of 3 M. The two substrates (banana peel and potato peel) were used for the amylase production among which banana peel showed that highest amylase activity of 6.12 U/mL than potato peel (4.27 U/mL).
本研究从海水中分离出8株盐杆菌。8株菌株中,4株在氯化钠浓度为40 g/100 mL的营养培养基中生长良好。其中,HA01的淀粉酶活性最高。产生最高淀粉酶的细菌,即经16S rRNA序列鉴定,HA01为水族细菌(aquabobacterium sp.)菌株AS02。在pH为7、温度为42°C、NaCl浓度为3 m的条件下,该菌产生淀粉酶的最佳条件为香蕉皮和马铃薯皮,其中香蕉皮的淀粉酶活性最高,为6.12 U/mL,马铃薯皮为4.27 U/mL。
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引用次数: 0
In-silico analysis of bioactive compounds extracted from seaweed Amphiroa anceps on the pathogenicity of bacteria 海藻类中生物活性成分对细菌致病性的影响
IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Pub Date : 2022-05-01 DOI: 10.56042/ijms.v51i05.65560
D. Madathil, R. Chidambaram, Jindal
Screening of biochemical compounds in marine algae is a gateway to understand the bioactive principles of antimicrobial activity and may be a significant tool in developing environment friendly herbicides and in drug discovery. In this study, the antibacterial activity of the seaweed Amphiroa anceps extracted with methanol and ethanol was determined against clinical isolates namely Vibrio cholerae 01 ogawa, Vibrio cholerae O139, Vibrio fluvialis , Vibrio parahaemolyticus , V. parahaemolyticus 81, V. parahaemolyticus O3:K6, salmonella enteritidis type 5, Enterotoxigenic E. coli , Bacillus cereus by disc diffusion method at different concentrations. Ethanolic extract showed the highest activity against V. parahaemolyticus , V. parahaemolyticus 81 and V. cholerae . The FTIR results showed the presence of major functional groups like amine, hydrogen bonded alcohols, alkanes and aromatic rings. Four major bioactive compounds were selected based on GC-MS data and used as ligands to dock in-silico against proteins of pathogenic bacteria that were used in antibiotic studies.
海洋藻类生物化学化合物的筛选是了解藻类抗菌活性的生物活性原理的重要途径,也是开发环境友好型除草剂和药物开发的重要工具。本研究采用圆盘扩散法测定了甲醇和乙醇提取的海藻类对不同浓度的临床分离株小川霍乱弧菌01、霍乱弧菌O139、流感弧菌、副溶血性弧菌81、副溶血性弧菌O3:K6、肠炎沙门氏菌5型、产肠毒素大肠杆菌、蜡样芽孢杆菌的抑菌活性。乙醇提取物对副溶血性弧菌、副溶血性弧菌81和霍乱弧菌的活性最高。FTIR结果表明,其主要官能团如胺、氢键醇、烷烃和芳香环的存在。根据GC-MS数据选择了四种主要的生物活性化合物,并将其作为配体与病原菌蛋白的硅对接,用于抗生素研究。
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引用次数: 1
Geochemistry of metals in grain size fractionated sediments from estuarine and coastal regions along Mumbai: A baseline information 孟买沿岸河口和沿海地区粒度沉积物中金属的地球化学:基线信息
IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Pub Date : 2022-01-01 DOI: 10.56042/ijms.v51i12.4290
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引用次数: 0
First report of Champsodon capensis Regan, 1908 (Champsodontidae) from East coast of India 标题印度东海岸Champsodon capensis Regan, 1908 (Champsodon dontidae)首次报告
IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Pub Date : 2022-01-01 DOI: 10.56042/ijms.v51i02.44480
D. Ray, T. Khatuaa, S. Royb, Anil Mohapatrab, Milani Mahavidyalaya, Purba, Medinipur
The gaper Champsodon capensis Regan, 1908 is reported for the first time from the east coast of India on the basis of 3 specimens (55 – 72 mm standard length) collected from Deshpran fishing harbor, West Bengal. Identification of the species is confirmed by ventral scale patterns on chin, breast, and abdomen. Earlier, this species was reported only from Andaman-Nicobar waters of India and the present finding report further range extension of the species to the northern part of the Bay of Bengal. This paper provides a detailed description of the species along with the comparison with other Champsodon species.
根据在西孟加拉邦Deshpran渔港采集的3个标本(标准长度55 ~ 72 mm),首次在印度东海岸发现了1908年的capensis Champsodon Regan。通过下巴、乳房和腹部的腹侧鳞片图案可以确定该物种的身份。早先,该物种仅在印度安达曼-尼科巴水域被报道,而目前的发现报告该物种的范围进一步扩大到孟加拉湾北部。本文对其种类进行了详细的描述,并与其他种类进行了比较。
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引用次数: 0
Reproductive biology of Pharaoh cuttlefish Sepia pharaonis Ehrenberg, 1831 along the Gujarat coast, India 法老墨鱼seia pharaonis Ehrenberg的生殖生物学,1831年在印度古吉拉特邦海岸
IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Pub Date : 2022-01-01 DOI: 10.56042/ijms.v51i11.3508
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引用次数: 0
Transportation of microplastic during high-flow and low-flow seasons in southeastern Black Sea: A modelling approach 黑海东南部高流量和低流量季节微塑料的迁移:一种模拟方法
IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Pub Date : 2022-01-01 DOI: 10.56042/ijms.v51i08.49207
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引用次数: 4
A comparative study of xylanase producing wild and mutated strains of Streptomyces variabilis VITMUVB02 isolated from Kanyakumari salt pan Kanyakumari盐田产木聚糖酶变异链霉菌(Streptomyces variabilis VITMUVB02)野生与突变菌株的比较研究
IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Pub Date : 2022-01-01 DOI: 10.56042/ijms.v51i10.2931
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引用次数: 0
Response of surface chlorophyll to aerosol dust input in the Central Arabian Sea 阿拉伯海中部海面叶绿素对气溶胶沙尘输入的响应
IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Pub Date : 2022-01-01 DOI: 10.56042/ijms.v51i04.35798
R. Roy, S. Prakashb, A. Lotlikerb, P. S. Sudhakarana, S. B. Choudhurya
The decadal trends in satellite-derived surface chlorophyll in conjunction with Aerosol Optical Depth (AOD) are explored in a unique area in the central Arabian Sea, known to mimic High Nutrient and Low Chlorophyll (HNLC) like conditions during late summer monsoon. The analysis indicates two recurring seasonal blooms, possibly associated with distinct biogeochemical processes in the studied region. Furthermore, the mineral dust deposition in July every year coincides with one such increase in surface chlorophyll followed by a lag period until winter monsoon. This rapid increase in the phytoplankton biomass just after the aeolian input is possibly due to an enhancement in soluble iron within the mixed layer, as suggested by AOD dust data. Most likely, this rapid increase in biomass may induce further depletion of soluble iron leading to HNLC-like conditions during the late Summer Monsoon, as reported earlier. This hypothesis is consistent with the satellite observation, which shows a decrease in surface chlorophyll during subsequent months until the convective mixing between December – January (winter monsoon). The study reveals that the presence of the HNLC region in the central Arabian Sea during the summer monsoon is not perennial like the Southern Ocean. Instead, it is a transient phenomenon primarily controlled by aerosol deposition and rapid uptake of soluble iron, which facilitate the diatom blooms as suggested by the recent output from the NASA Ocean Biogeochemical Model (NOBM).
在阿拉伯海中部的一个独特区域,结合气溶胶光学深度(AOD),研究了卫星获得的地表叶绿素的年年趋势,该区域在夏末季风期间模仿高营养和低叶绿素(HNLC)的情况。分析表明,在研究地区有两次季节性的水华,可能与不同的生物地球化学过程有关。此外,每年7月的矿物粉尘沉积与地表叶绿素的增加相吻合,然后是一个滞后期,直到冬季季风。正如AOD粉尘数据所表明的那样,在风沙输入后浮游植物生物量的快速增加可能是由于混合层中可溶性铁的增加。最有可能的是,如前所述,这种生物量的快速增加可能会导致可溶性铁的进一步消耗,从而在夏末季风期间导致类似hnlc的情况。这一假设与卫星观测相一致,卫星观测显示,在随后的几个月里,直到12月至1月(冬季季风)之间的对流混合,地表叶绿素都在减少。研究表明,夏季风期间阿拉伯海中部HNLC区域的存在不像南大洋那样是常年存在的。相反,这是一种暂时的现象,主要由气溶胶沉积和可溶性铁的快速吸收控制,正如美国宇航局海洋生物地球化学模型(NOBM)最近的结果所表明的那样,这促进了硅藻的繁殖。
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引用次数: 0
Limemud resources in the Western Indian offshore: An alternative to high grade limestone and some viable proposals 西印度近海的石灰石资源:高品位石灰石的替代品和一些可行的建议
IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Pub Date : 2022-01-01 DOI: 10.56042/ijms.v51i09.2338
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引用次数: 0
Studies on nutritional composition of three colour forms of Kappaphycus alvarezii (Doty) Doty 三种颜色形态木皮的营养成分研究
IF 0.5 4区 地球科学 Q4 Earth and Planetary Sciences Pub Date : 2022-01-01 DOI: 10.56042/ijms.v51i01.45173
Doty, K. Kumar, Sushma Kumaria, P. V. Raob
Biochemical composition of seaweeds is known to vary with species and environment. Kappaphycus alvarezii is an important commercial source of carrageenans (gel-forming and viscosifying polysaccharides). The nutritional and mineral composition of three (brown, green and pale yellow) colour forms of edible seaweed K. alvarezii is investigated in the current study. The brown colour form of this seaweed contained highest ash content of (25.99±0.22 %), crude fibre (21.0±0.68 %), and protein (8.92±0.41 %), while, the green form had highest carbohydrate (22.0±0.00 %) and sulphate (5.89±0.00 %) content. On the other hand, the pale yellow colour form had highest lipid content (0.72±0.00 %). Amongst the 17 minerals analyzed, highest total mineral macro-elements ( viz . Na, K, Ca and Mg) were recorded in the brown form (18.8±0.71 g/100 g d wt) followed by the pale yellow (17.3±1.07 g/100 g d wt) and green colour form (11.9±1.22 g/100 g d wt). The micro-elements varied within the three colour forms; however, the brown colour form had maximum micro-element content (P, Cd, Pb, As, Hg, Cr, Zn, Cu, Fe, Mn, Co, Mo and Ni; 19.96±0.24mg/100 g d wt), followed by green (8.6±0.64 mg /100 g d wt) and pale yellow form (7.15±0.58mg /100 g d wt). On the basis of this study, it could be said that these three colour forms ( i.e . brown, green and pale yellow) of Kappaphycus alvarezii could be utilized as a condiment in the omnivorous diet; however, a daily intake of 5.68 g d wt is recommended in case of the brown form, while in case of the green and pale yellow form a maximum daily intake of 9.55 and 10.5 g d wt, respectively is recommended. The study also reveals that the three colour-forms differed in their nutritional and mineral composition.
众所周知,海藻的生化成分随种类和环境而变化。木耳是卡拉胶(凝胶和增粘多糖)的重要商业来源。本文研究了三种(棕色、绿色和淡黄色)可食用海藻的营养和矿物质组成。褐藻的灰分(25.99±0.22%)、粗纤维(21.0±0.68%)和蛋白质(8.92±0.41%)含量最高,绿藻的碳水化合物(22.0±0.00 %)和硫酸盐(5.89±0.00 %)含量最高。另一方面,浅黄色形态的脂肪含量最高(0.72±0.00 %)。在所分析的17种矿物中,总矿物质常量元素(即Na, K, Ca和Mg)以棕色形式记录(18.8±0.71 g/100 g d wt),其次是淡黄色形式(17.3±1.07 g/100 g d wt)和绿色形式(11.9±1.22 g/100 g d wt)。微量元素在三种颜色形式中有所不同;而棕色形态的微量元素(P、Cd、Pb、As、Hg、Cr、Zn、Cu、Fe、Mn、Co、Mo和Ni)含量最高;19.96±0.24mg/100 g d wt),其次是绿色(8.6±0.64 mg/100 g d wt)和淡黄色(7.15±0.58mg /100 g d wt)。在此研究的基础上,可以说这三种颜色形式(即:(褐色、绿色和淡黄色)可作为杂食性食物的调味品;然而,建议每天摄入5.68 g d wt的褐色形式,而对于绿色和淡黄色形式的最大每日摄入量分别为9.55 g和10.5 g d wt。该研究还揭示了三种颜色形式在营养和矿物质成分上的不同。
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Indian Journal of Geo-Marine Sciences
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