The U.S. National Cancer Institute's Division of Cancer Epidemiology and Genetics (DCEG) conducts population-based and interdisciplinary research to discover the genetic and environmental determinants of cancer. Many DCEG studies are large, multi-institutional, and long-term with national and international study sites involved in the multiple research steps. Current information technology challenges involved in such epidemiological studies include: (1) management and harmonization of a multitude of data types (demographic, environmental, biospecimen, laboratory, analytic, molecular, etc.); (2) unprecedented amounts of data; (3) efficient data mining to derive insights into disease etiology; and (4) secure collaboration between study management systems. If not adequately addressed, all of these challenges will increase the cost of performing studies and decrease the speed of publication. DCEG is examining current data management practices to better utilize recent advances in information technology to enhan...
{"title":"Challenges of Scientific Data Management for Large Epidemiologic Studies","authors":"M. Henderson, C. Mohla, K. Jacobs, J. Vaught","doi":"10.1089/CPT.2005.3.49","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.49","url":null,"abstract":"The U.S. National Cancer Institute's Division of Cancer Epidemiology and Genetics (DCEG) conducts population-based and interdisciplinary research to discover the genetic and environmental determinants of cancer. Many DCEG studies are large, multi-institutional, and long-term with national and international study sites involved in the multiple research steps. Current information technology challenges involved in such epidemiological studies include: (1) management and harmonization of a multitude of data types (demographic, environmental, biospecimen, laboratory, analytic, molecular, etc.); (2) unprecedented amounts of data; (3) efficient data mining to derive insights into disease etiology; and (4) secure collaboration between study management systems. If not adequately addressed, all of these challenges will increase the cost of performing studies and decrease the speed of publication. DCEG is examining current data management practices to better utilize recent advances in information technology to enhan...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"2005-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.49","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Within many biomedical engineering subspecialties, there is a need for the reversible developmental arrest of cells and tissues in support of global distribution followed by on-demand restoration of cellular function (biopreservation). Recently, the field of anhydrobiotic (dry-state) preservation has emerged as a potential approach to achieve this goal. To date, investigations have focused on different approaches utilizing stabilizing molecules, such as trehalose, to achieve anhydrobiotic preservation, yet there remains a void in the understanding of the molecular-based physiological and biochemical responses of biologics to the desiccation process. Accordingly, we formulated and tested the hypothesis that apoptosis contributes to cell death following anhydrobiotic preservation through the activation of multiple pathways by the complex array of stressors associated with the desiccation process. In addition, we investigated the modulation of cell death following desiccation, hypothesizing that through inhi...
{"title":"Induction of Apoptosis in Response to Anhydrobiotic Conditions in Mammalian Cells","authors":"J. Baust, A. Fowler, M. Toner","doi":"10.1089/CPT.2004.2.248","DOIUrl":"https://doi.org/10.1089/CPT.2004.2.248","url":null,"abstract":"Within many biomedical engineering subspecialties, there is a need for the reversible developmental arrest of cells and tissues in support of global distribution followed by on-demand restoration of cellular function (biopreservation). Recently, the field of anhydrobiotic (dry-state) preservation has emerged as a potential approach to achieve this goal. To date, investigations have focused on different approaches utilizing stabilizing molecules, such as trehalose, to achieve anhydrobiotic preservation, yet there remains a void in the understanding of the molecular-based physiological and biochemical responses of biologics to the desiccation process. Accordingly, we formulated and tested the hypothesis that apoptosis contributes to cell death following anhydrobiotic preservation through the activation of multiple pathways by the complex array of stressors associated with the desiccation process. In addition, we investigated the modulation of cell death following desiccation, hypothesizing that through inhi...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"23 1","pages":"248-259"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2004.2.248","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effects of growth media, drying protocols, and storage conditions on the long-term survival and activity of Escherichia coli, which harbors genetic fusions of inducer-responsive promoters to bacterial luminescence (lux) genes, were evaluated. Bacteria were grown in a regular or high-salinity (0.5 M NaCl) complex medium (Luria–Bertani, LB) or minimal medium (M-9), freeze-dried or dried by vacuum under ambient temperatures, and stored at –20°C or 37°C. The survival rates of vacuum-dried and freeze-dried bacteria grown in LB ranged from 1% to 2% and 19% to 20%, respectively, and were not as affected by medium salinity. Survival rates of vacuum-dried and freeze-dried bacteria grown in M-9 ranged from 22% to 75% and 45% to 63%, respectively. Here, however, high salinity promoted survival of vacuum-dried bacteria but negatively affected the survival of freeze-dried bacteria. Survival rates of vacuum-dried bacteria stored at 37°C were substantially higher than those of freeze-dried bacteria and were maximal ...
{"title":"Stabilization of Recombinant Bioluminescent Bacteria for Biosensor Applications","authors":"R. Pedahzur, R. Rosen, S. Belkin","doi":"10.1089/CPT.2004.2.260","DOIUrl":"https://doi.org/10.1089/CPT.2004.2.260","url":null,"abstract":"The effects of growth media, drying protocols, and storage conditions on the long-term survival and activity of Escherichia coli, which harbors genetic fusions of inducer-responsive promoters to bacterial luminescence (lux) genes, were evaluated. Bacteria were grown in a regular or high-salinity (0.5 M NaCl) complex medium (Luria–Bertani, LB) or minimal medium (M-9), freeze-dried or dried by vacuum under ambient temperatures, and stored at –20°C or 37°C. The survival rates of vacuum-dried and freeze-dried bacteria grown in LB ranged from 1% to 2% and 19% to 20%, respectively, and were not as affected by medium salinity. Survival rates of vacuum-dried and freeze-dried bacteria grown in M-9 ranged from 22% to 75% and 45% to 63%, respectively. Here, however, high salinity promoted survival of vacuum-dried bacteria but negatively affected the survival of freeze-dried bacteria. Survival rates of vacuum-dried bacteria stored at 37°C were substantially higher than those of freeze-dried bacteria and were maximal ...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"2 1","pages":"260-269"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2004.2.260","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cryopreservation using freeze–thaw methods is one way in which cells can be safely stored at low temperatures for indefinite periods of time. Unfortunately, the process itself can damage the cells such that the number of viable cells after thawing is reduced. This problem is particularly important when dealing with nonproliferating cells, such as primary hepatocytes. In the current study, directional solidification is used to help improve success when cooling hepatocytes to subzero temperatures. Specifically, by analyzing the crystallization of solutions, cell suspensions, and tissue cultures in the presence of dimethyl sulfoxide (DMSO), we determine how changes in the morphology and rate of crystal growth influence cell survival. The results demonstrate that the presence of extracellular matrix (ECM) and alterations in DMSO concentration are two ways to affect cell viability. Next, by modeling the directional solidification results as a Stefan–Neumann Problem, the image analysis data is then used to quan...
{"title":"Use of Directional Solidification to Quantify the Thermophysical Properties of DMSO-based Cryoprotectant Solutions","authors":"Yekaterina S. Zinchenko, E. Laureano, R. Coger","doi":"10.1089/CPT.2004.2.276","DOIUrl":"https://doi.org/10.1089/CPT.2004.2.276","url":null,"abstract":"Cryopreservation using freeze–thaw methods is one way in which cells can be safely stored at low temperatures for indefinite periods of time. Unfortunately, the process itself can damage the cells such that the number of viable cells after thawing is reduced. This problem is particularly important when dealing with nonproliferating cells, such as primary hepatocytes. In the current study, directional solidification is used to help improve success when cooling hepatocytes to subzero temperatures. Specifically, by analyzing the crystallization of solutions, cell suspensions, and tissue cultures in the presence of dimethyl sulfoxide (DMSO), we determine how changes in the morphology and rate of crystal growth influence cell survival. The results demonstrate that the presence of extracellular matrix (ECM) and alterations in DMSO concentration are two ways to affect cell viability. Next, by modeling the directional solidification results as a Stefan–Neumann Problem, the image analysis data is then used to quan...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"2 1","pages":"276-289"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2004.2.276","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. J. Barnett, G. Cembrowski, A. M. Shapiro, J. Lakey
The use of University of Wisconsin (UW) solution is widely accepted for the cold storage and transport of human donor pancreata destined for islet isolation. While several variables exist in islet isolation, proper procurement and packaging is essential for optimizing preprocessing conditions. This study examined the effect of normal saline (NS) contamination/dilution of transport UW solution in 89 human pancreata processed by our islet isolation program. Samples of UW were taken from the primary pancreas container and examined for the presence of blood and or hemolysis. The cold ischemia time (CIT) was calculated, and processing by cold collagenase perfusion commenced. Islets were then isolated by collagenase digestion, purified by continuous gradient Ficoll centrifugation, and islet equivalent (IE) yield determined by dithizone staining. Viability of isolated islets was assayed by using the fluorescent SYTO/ethidium bromide (SYTO/EB) method. Na+ and K+ values were assayed using ion-specific electrode me...
{"title":"Saline Contamination of University of Wisconsin Solution Preserved Human Pancreata","authors":"M. J. Barnett, G. Cembrowski, A. M. Shapiro, J. Lakey","doi":"10.1089/CPT.2004.2.290","DOIUrl":"https://doi.org/10.1089/CPT.2004.2.290","url":null,"abstract":"The use of University of Wisconsin (UW) solution is widely accepted for the cold storage and transport of human donor pancreata destined for islet isolation. While several variables exist in islet isolation, proper procurement and packaging is essential for optimizing preprocessing conditions. This study examined the effect of normal saline (NS) contamination/dilution of transport UW solution in 89 human pancreata processed by our islet isolation program. Samples of UW were taken from the primary pancreas container and examined for the presence of blood and or hemolysis. The cold ischemia time (CIT) was calculated, and processing by cold collagenase perfusion commenced. Islets were then isolated by collagenase digestion, purified by continuous gradient Ficoll centrifugation, and islet equivalent (IE) yield determined by dithizone staining. Viability of isolated islets was assayed by using the fluorescent SYTO/ethidium bromide (SYTO/EB) method. Na+ and K+ values were assayed using ion-specific electrode me...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"2 1","pages":"290-295"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2004.2.290","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biorepositories in Clinical and Basic ResearchOctober 17–20, 2004University of PerugiaPerugia, Italy","authors":"R. Hanner, P. Blasio","doi":"10.1089/CPT.2004.2.296","DOIUrl":"https://doi.org/10.1089/CPT.2004.2.296","url":null,"abstract":"","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"92 1","pages":"296-297"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2004.2.296","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60911787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jianping Yu, J. H. Liu, L. Q. Pu, X. Cui, Changzheng Wang, S. Ouyang, D. Gao
Lyophilization (freeze-drying) is one potential approach for long-term preservation of biological materials such as human platelets and erythrocytes (red blood cells; RBCs). It has advantages in comparison with conventional cryopreservation, including the elimination of the need for storage at low temperatures (low cost), and reduced weight (easy for storing and shipping). However, recovery of RBC viability and function after lyophilization has been generally low, as detailed in previous reports and patents. Many factors may influence the recovery of cell viability during the freeze-drying process. Such factors include the lyoprotectants (sugars, polymers), the operating condition during the drying process (primary and secondary drying), and the rehydration process. In this study, the influence of the sugars (as lyoprotectants) on the RBC recovery after lyophilization has been investigated. Cell integrity, hematocrit (HCT), and hemoglobin content were used to evaluate the recovery rate after lyophilizatio...
{"title":"Freeze-drying of Human Red Blood Cells: Influence of Carbohydrates and Their Concentrations","authors":"Jianping Yu, J. H. Liu, L. Q. Pu, X. Cui, Changzheng Wang, S. Ouyang, D. Gao","doi":"10.1089/CPT.2004.2.270","DOIUrl":"https://doi.org/10.1089/CPT.2004.2.270","url":null,"abstract":"Lyophilization (freeze-drying) is one potential approach for long-term preservation of biological materials such as human platelets and erythrocytes (red blood cells; RBCs). It has advantages in comparison with conventional cryopreservation, including the elimination of the need for storage at low temperatures (low cost), and reduced weight (easy for storing and shipping). However, recovery of RBC viability and function after lyophilization has been generally low, as detailed in previous reports and patents. Many factors may influence the recovery of cell viability during the freeze-drying process. Such factors include the lyoprotectants (sugars, polymers), the operating condition during the drying process (primary and secondary drying), and the rehydration process. In this study, the influence of the sugars (as lyoprotectants) on the RBC recovery after lyophilization has been investigated. Cell integrity, hematocrit (HCT), and hemoglobin content were used to evaluate the recovery rate after lyophilizatio...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"2 1","pages":"270-275"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2004.2.270","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giuliana Saltini, M. Frequin, C. Oliveri, P. Rossi, J. Coccia, C. Beiswanger, P. Blasio, I. Biunno
{"title":"Lymphoblasts Biorepository : building up,management, and quality control to improve medical research","authors":"Giuliana Saltini, M. Frequin, C. Oliveri, P. Rossi, J. Coccia, C. Beiswanger, P. Blasio, I. Biunno","doi":"10.1089/CPT.2004.2.298","DOIUrl":"https://doi.org/10.1089/CPT.2004.2.298","url":null,"abstract":"","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"2 1","pages":"330-330"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2004.2.298","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60911872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thurein Htoo, Kamran Jamil, J. Crowe, F. Tablin, A. E. Oliver
Recent advances in the stabilization of eukaryotic cells during desiccation provide possibilities for present and future pharmaceutical and biomedical applications. Such applications require strict adherence to sterility. Devices for the sterile processing of biological samples during drying and rehydration are lacking, however. An ideal device suitable for these procedures, especially for use in freeze-drying, would maintain sterility, allow sterile temperature sampling during the drying procedure, allow for microscopic analysis in the dry and rehydrated state, and allow for subsequent use in cell and tissue culture following rehydration. Furthermore, since the initial hydration of samples from the vapor phase (pre-hydration) is often useful to preserve sample integrity prior to complete rehydration, the device must prevent contamination during this process. We have developed a device suitable for these tasks. The Biomaterial Desiccation, Rehydration, and Imaging Flask (BioDRI™ Flask) fulfills all of the...
{"title":"Flask for the Drying of Biological Material","authors":"Thurein Htoo, Kamran Jamil, J. Crowe, F. Tablin, A. E. Oliver","doi":"10.1089/CPT.2004.2.172","DOIUrl":"https://doi.org/10.1089/CPT.2004.2.172","url":null,"abstract":"Recent advances in the stabilization of eukaryotic cells during desiccation provide possibilities for present and future pharmaceutical and biomedical applications. Such applications require strict adherence to sterility. Devices for the sterile processing of biological samples during drying and rehydration are lacking, however. An ideal device suitable for these procedures, especially for use in freeze-drying, would maintain sterility, allow sterile temperature sampling during the drying procedure, allow for microscopic analysis in the dry and rehydrated state, and allow for subsequent use in cell and tissue culture following rehydration. Furthermore, since the initial hydration of samples from the vapor phase (pre-hydration) is often useful to preserve sample integrity prior to complete rehydration, the device must prevent contamination during this process. We have developed a device suitable for these tasks. The Biomaterial Desiccation, Rehydration, and Imaging Flask (BioDRI™ Flask) fulfills all of the...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"2 1","pages":"172-179"},"PeriodicalIF":0.0,"publicationDate":"2004-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2004.2.172","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60911466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The formation of ice crystals is known to be lethal to biological cells. The presence of cryoprotectants at high cooling rates suppresses crystallization and promotes vitrification, where vitrification is solidification by rapid elevation of the viscosity (vitreous, in Latin, means glass). All materials have a tendency to change volume with a change in temperature, where the rate at which the volume changes with respect to the temperature is defined as the thermophysical property of thermal expansion. In the presence of a non-uniform temperature distribution in a bulky specimen, when different regions of the material tend to expand differently, mechanical stress may develop. It has been demonstrated that this mechanical stress can easily lead to macro structural damage to the cryopreserved specimen. As part of an ongoing effort to characterize the mechanical behavior of biological tissues and solutions in the cryogenic temperature range, the current study focuses on mapping the thermal expansion effect of...
{"title":"The Effect of Thermal Expansion of Ingredients on the Cocktails VS55 and DP6","authors":"Joe Plitz, Y. Rabin, J. Walsh","doi":"10.1089/CPT.2004.2.215","DOIUrl":"https://doi.org/10.1089/CPT.2004.2.215","url":null,"abstract":"The formation of ice crystals is known to be lethal to biological cells. The presence of cryoprotectants at high cooling rates suppresses crystallization and promotes vitrification, where vitrification is solidification by rapid elevation of the viscosity (vitreous, in Latin, means glass). All materials have a tendency to change volume with a change in temperature, where the rate at which the volume changes with respect to the temperature is defined as the thermophysical property of thermal expansion. In the presence of a non-uniform temperature distribution in a bulky specimen, when different regions of the material tend to expand differently, mechanical stress may develop. It has been demonstrated that this mechanical stress can easily lead to macro structural damage to the cryopreserved specimen. As part of an ongoing effort to characterize the mechanical behavior of biological tissues and solutions in the cryogenic temperature range, the current study focuses on mapping the thermal expansion effect of...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"2 1","pages":"215-226"},"PeriodicalIF":0.0,"publicationDate":"2004-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2004.2.215","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：