Paul S Steif, Matthew Palastro, Chen-Rei Wan, Simona Baicu, Michael J Taylor, Yoed Rabin
A new imaging device, termed a "cryomacroscope", was used to observe macrofractures in the cryoprotectant cocktails DP6 and VS55. Details of the design and construction of the cryomacroscope were presented in Part I of this report, which focused on describing the apparatus and observations of crystallization. Part I and the current paper (Part II) describe events that occur as 1 mℓ of cryoprotectant contained in a glass vial is cooled from room temperature down to cryogenic temperatures (∼ -135°C). The presence of cracking, as well as patterns in their position and orientation, are found to be dependent on the cooling rate and on the specific cryoprotectant cocktail. Cracks, if present, disappear upon rewarming, although they appear to be sites for later preferential crystallization. Computations which predict temperatures and mechanical stresses are used to explain observations of cracking. In conjunction with these reports, additional photos of cryomacroscopy of vitrification, crystallization, and fracture formation are available at http://www.me.cmu.edu/faculty1/rabin/CryomacroscopyImages01.htm.
一种新的成像设备，称为“低温显微镜”，用于观察低温保护剂DP6和VS55的大断裂。本报告第一部分详细介绍了低温显微镜的设计和构造，重点描述了结晶装置和观察结果。第一部分和当前的论文(第二部分)描述了当玻璃小瓶中含有1 μ m μ l的冷冻保护剂从室温冷却到低温(~ -135℃)时发生的事件。裂缝的存在，以及它们的位置和方向的模式，被发现取决于冷却速度和特定的冷冻保护剂混合物。裂纹如果存在，则在再加热时消失，尽管它们似乎是以后优先结晶的场所。预测温度和机械应力的计算被用来解释裂缝的观察结果。与这些报告相结合，更多的玻璃化、结晶和裂缝形成的低温显微镜照片可在http://www.me.cmu.edu/faculty1/rabin/CryomacroscopyImages01.htm上获得。
{"title":"Cryomacroscopy of vitrification, Part II: Experimental observations and analysis of fracture formation in vitrified VS55 and DP6.","authors":"Paul S Steif, Matthew Palastro, Chen-Rei Wan, Simona Baicu, Michael J Taylor, Yoed Rabin","doi":"10.1089/cpt.2005.3.184","DOIUrl":"https://doi.org/10.1089/cpt.2005.3.184","url":null,"abstract":"<p><p>A new imaging device, termed a \"cryomacroscope\", was used to observe macrofractures in the cryoprotectant cocktails DP6 and VS55. Details of the design and construction of the cryomacroscope were presented in Part I of this report, which focused on describing the apparatus and observations of crystallization. Part I and the current paper (Part II) describe events that occur as 1 mℓ of cryoprotectant contained in a glass vial is cooled from room temperature down to cryogenic temperatures (∼ -135°C). The presence of cracking, as well as patterns in their position and orientation, are found to be dependent on the cooling rate and on the specific cryoprotectant cocktail. Cracks, if present, disappear upon rewarming, although they appear to be sites for later preferential crystallization. Computations which predict temperatures and mechanical stresses are used to explain observations of cracking. In conjunction with these reports, additional photos of cryomacroscopy of vitrification, crystallization, and fracture formation are available at http://www.me.cmu.edu/faculty1/rabin/CryomacroscopyImages01.htm.</p>","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 3","pages":"184-200"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/cpt.2005.3.184","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26193921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yoed Rabin, Michael J Taylor, John R Walsh, Simona Baicu, Paul S Steif
A new imaging device, termed a "cryomacroscope", is presented in this report. This device is designed to assist in exploring thermal and mechanical effects associated with large-scale vitrification and crystallization, with the current setup aimed at the range of 50 μm to 2 cm. The cryomacroscope is not intended as a substitute for the cryomicroscope, but as a complementary tool for the cryobiologist. A combination of cryomacroscopy and cryomicroscopy is suggested as a basis for multi-scale cryobiology studies. This report presents initial results on vitrification, crystallization, and fracture formation in the cryoprotectant cocktails DP6 and VS55. These results show some inconsistency in the tendency to form crystals, based on critical cooling and rewarming rates measured by means of a differential scanning calorimetric device (DSC) in parallel studies. This research is in its early stages, and comparative studies on biological materials are currently underway. Part II of this report (the companion paper) presents results for fracture formation in the cryoprotectant and discusses the mechanical stresses which promote these fractures. In conjunction with these reports, additional photos of cryomacroscopy of vitrification, crystallization, and fracture formation are available at http://www.me.cmu.edu/faculty1/rabin/CryomacroscopyImages01.htm.
{"title":"Cryomacroscopy of vitrification, Part I: A prototype and experimental observations on the cocktails VS55 and DP6.","authors":"Yoed Rabin, Michael J Taylor, John R Walsh, Simona Baicu, Paul S Steif","doi":"10.1089/cpt.2005.3.169","DOIUrl":"https://doi.org/10.1089/cpt.2005.3.169","url":null,"abstract":"<p><p>A new imaging device, termed a \"cryomacroscope\", is presented in this report. This device is designed to assist in exploring thermal and mechanical effects associated with large-scale vitrification and crystallization, with the current setup aimed at the range of 50 μm to 2 cm. The cryomacroscope is not intended as a substitute for the cryomicroscope, but as a complementary tool for the cryobiologist. A combination of cryomacroscopy and cryomicroscopy is suggested as a basis for multi-scale cryobiology studies. This report presents initial results on vitrification, crystallization, and fracture formation in the cryoprotectant cocktails DP6 and VS55. These results show some inconsistency in the tendency to form crystals, based on critical cooling and rewarming rates measured by means of a differential scanning calorimetric device (DSC) in parallel studies. This research is in its early stages, and comparative studies on biological materials are currently underway. Part II of this report (the companion paper) presents results for fracture formation in the cryoprotectant and discusses the mechanical stresses which promote these fractures. In conjunction with these reports, additional photos of cryomacroscopy of vitrification, crystallization, and fracture formation are available at http://www.me.cmu.edu/faculty1/rabin/CryomacroscopyImages01.htm.</p>","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 3","pages":"169-183"},"PeriodicalIF":0.0,"publicationDate":"2005-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/cpt.2005.3.169","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26040847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The resistance of electronic information stored on cryotags affixed to mock biological materials kept at very low temperatures (–80°C or –196°C) was tested over a period of 18 months. We found excellent performance for reading and re-writing tags maintained in these hostile conditions. Radiofrequency identification (RFID) technology may help solve some of the problems that biobanks face in identifying their biological samples and exchanging related information.
{"title":"RFID Technology and Electronic Tags to Identify Cryopreserved Materials","authors":"Emmanuelle Bettendorf, C. Malenfant, C. Chabannon","doi":"10.1089/CPT.2005.3.112","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.112","url":null,"abstract":"The resistance of electronic information stored on cryotags affixed to mock biological materials kept at very low temperatures (–80°C or –196°C) was tested over a period of 18 months. We found excellent performance for reading and re-writing tags maintained in these hostile conditions. Radiofrequency identification (RFID) technology may help solve some of the problems that biobanks face in identifying their biological samples and exchanging related information.","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"112-115"},"PeriodicalIF":0.0,"publicationDate":"2005-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60911956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tony Alan Ratliff, G. Satpathy, M. Banerjee, Rachna Bali, Erika Little, Roberta Novaes, H. Ly, D. Dwyre, A. Kheirolomoom, F. Tablin, J. Crowe, N. Tsvetkova
A method for freeze-drying red blood cells (RBCs) while maintaining high viability has important implications in blood transfusion and clinical medicine. RBCs loaded with the disaccharide trehalose can be freeze-dried in a formulation of hydroxyethyl starch, human serum albumin, and trehalose to residual water contents between 2% and 4%. Rehydration of the freeze-dried erythrocytes resulted in about 55% survival, based on the percent hemolysis. The surviving cells can synthesize ATP and 2,3-DPG, have preserved morphology, and have low levels of methemoglobin. Biochemical analyses showed that the activities of superoxide dismutase and catalase in freezedried RBCs are very similar to those of fresh RBCs. Secondary structure of hemoglobin was similar to that of fresh hemoglobin. Trehalose loading is required to achieve the stabilization of hemoglobin. These data provide an important step towards a stable erythrocyte product, which can prove invaluable for transfusion and clinical applications.
{"title":"Preservation of Trehalose-Loaded Red Blood Cells by Lyophilization","authors":"Tony Alan Ratliff, G. Satpathy, M. Banerjee, Rachna Bali, Erika Little, Roberta Novaes, H. Ly, D. Dwyre, A. Kheirolomoom, F. Tablin, J. Crowe, N. Tsvetkova","doi":"10.1089/CPT.2005.3.96","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.96","url":null,"abstract":"A method for freeze-drying red blood cells (RBCs) while maintaining high viability has important implications in blood transfusion and clinical medicine. RBCs loaded with the disaccharide trehalose can be freeze-dried in a formulation of hydroxyethyl starch, human serum albumin, and trehalose to residual water contents between 2% and 4%. Rehydration of the freeze-dried erythrocytes resulted in about 55% survival, based on the percent hemolysis. The surviving cells can synthesize ATP and 2,3-DPG, have preserved morphology, and have low levels of methemoglobin. Biochemical analyses showed that the activities of superoxide dismutase and catalase in freezedried RBCs are very similar to those of fresh RBCs. Secondary structure of hemoglobin was similar to that of fresh hemoglobin. Trehalose loading is required to achieve the stabilization of hemoglobin. These data provide an important step towards a stable erythrocyte product, which can prove invaluable for transfusion and clinical applications.","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"96-111"},"PeriodicalIF":0.0,"publicationDate":"2005-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.96","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60913014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Peitao Wang, Z. Shu, Liqun He, Yuzhen Wang, X. Cui, Jianping Yu, Junfeng Lu, D. Gao
New techniques have been developed for cryopreservation of arteries for use in transplantations or bypass surgery. In our experiments, rabbit carotid arteries were cryopreserved in a cryoprotective medium containing 1.5 M 1,2- propanediol (PROH). After storage in liquid nitrogen for over 10 days, the frozen arteries were thawed slowly in an ice bag that had been pre-cooled in liquid nitrogen to prevent thermal stress and fracture. Fresh carotids were used as normal controls. The fresh and cryopreserved arteries were cultured. The growth of smooth muscle cells (SMCs), the development of endothelial cells (ECs), the integrity of carotid walls (the elastic lamellae or fibers), and the mechanical properties of arteries (elastic modulus and fracture strength) were investigated. The results showed that SMCs survived cryopreservation. It took approximately the same amount of time (∼24–36 h) for the SMCs of cryopreserved arteries to regenerate as those of the fresh arteries. After cryopreservation, only a small n...
为了在移植或搭桥手术中使用冷冻保存动脉的新技术已经被开发出来。在我们的实验中，将兔颈动脉冷冻保存在含有1.5 M 1,2-丙二醇(PROH)的冷冻保护介质中。冷冻动脉在液氮中保存10天以上后，放入液氮中预冷的冰袋中缓慢解冻，以防止热应力和断裂。新鲜类胡萝卜素作为正常对照。分别培养新鲜动脉和冷冻动脉。观察平滑肌细胞(SMCs)的生长、内皮细胞(ECs)的发育、颈动脉壁(弹性片层或纤维)的完整性以及动脉的力学性能(弹性模量和断裂强度)。结果表明，SMCs在低温保存下存活。冷冻保存动脉的SMCs与新鲜动脉的SMCs再生所需的时间大致相同(~ 24-36小时)。冷冻保存后，只有一小部分
{"title":"The Viability, Structure, and Mechanical Properties of Cryopreserved Rabbit Carotid Artery","authors":"Peitao Wang, Z. Shu, Liqun He, Yuzhen Wang, X. Cui, Jianping Yu, Junfeng Lu, D. Gao","doi":"10.1089/CPT.2005.3.85","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.85","url":null,"abstract":"New techniques have been developed for cryopreservation of arteries for use in transplantations or bypass surgery. In our experiments, rabbit carotid arteries were cryopreserved in a cryoprotective medium containing 1.5 M 1,2- propanediol (PROH). After storage in liquid nitrogen for over 10 days, the frozen arteries were thawed slowly in an ice bag that had been pre-cooled in liquid nitrogen to prevent thermal stress and fracture. Fresh carotids were used as normal controls. The fresh and cryopreserved arteries were cultured. The growth of smooth muscle cells (SMCs), the development of endothelial cells (ECs), the integrity of carotid walls (the elastic lamellae or fibers), and the mechanical properties of arteries (elastic modulus and fracture strength) were investigated. The results showed that SMCs survived cryopreservation. It took approximately the same amount of time (∼24–36 h) for the SMCs of cryopreserved arteries to regenerate as those of the fresh arteries. After cryopreservation, only a small n...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"85-95"},"PeriodicalIF":0.0,"publicationDate":"2005-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.85","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Pitt, Lori D. Campbell, A. Skubitz, R. Aamodt, A. Anouna, Philip M Baird, J. Beck, M. Bledsoe, Yvonne De Souza, W. Grizzle, Jaya Gosh, N. Holland, R. Hakimian, Cheryl Michels, Katherine C. Sexton, Kathi Shea, Azadeh T. Stark, J. Vaught
THE DESIRE TO PRESERVE biological and environmental specimens for research purposes and to ensure species biodiversity require the development of methods for long-term storage that will enable their effective future use. Sharing successful strategies for accomplishing this goal was one of the early driving forces for the International Society for Biological and Environmental Repositories (ISBER). In addition, ISBER fosters education and research and promotes quality and safety in all activities relating to specimen collection, storage and dissemination. ISBER’s Best Practices for Repositories (Best Practices) reflect the collective experience of its members to provide repository professionals with a comprehensive foundation for the guidance of repository activities. These Practices reflect input from individuals within and outside ad hoc committees. Best Practices will be reviewed periodically and will be revised to reflect advances in research and technology. All revisions are subject to approval by the ISBER Council. These Practices reflect the most effective approaches to the establishment and running of specimen collection facilities and are not intended as required practices. The focus of this first version of the ISBER Best Practices is on the management of human specimen collections. It is ISBER’s intention to broaden the focus to include Best Practices surrounding other specimen types in subsequent versions. Likewise, because most of the initial contributions to this edition were from individuals based in the United States, the Practices described here primarily reflect U.S. perspectives. ISBER plans to broaden the scope of the Practices in future editions to include those from other nations in order to make this document more representative of international perspectives and experience.
{"title":"Best practices for repositories I: Collection, storage, and retrieval of human biological materials for research","authors":"K. Pitt, Lori D. Campbell, A. Skubitz, R. Aamodt, A. Anouna, Philip M Baird, J. Beck, M. Bledsoe, Yvonne De Souza, W. Grizzle, Jaya Gosh, N. Holland, R. Hakimian, Cheryl Michels, Katherine C. Sexton, Kathi Shea, Azadeh T. Stark, J. Vaught","doi":"10.1089/CPT.2005.3.5","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.5","url":null,"abstract":"THE DESIRE TO PRESERVE biological and environmental specimens for research purposes and to ensure species biodiversity require the development of methods for long-term storage that will enable their effective future use. Sharing successful strategies for accomplishing this goal was one of the early driving forces for the International Society for Biological and Environmental Repositories (ISBER). In addition, ISBER fosters education and research and promotes quality and safety in all activities relating to specimen collection, storage and dissemination. ISBER’s Best Practices for Repositories (Best Practices) reflect the collective experience of its members to provide repository professionals with a comprehensive foundation for the guidance of repository activities. These Practices reflect input from individuals within and outside ad hoc committees. Best Practices will be reviewed periodically and will be revised to reflect advances in research and technology. All revisions are subject to approval by the ISBER Council. These Practices reflect the most effective approaches to the establishment and running of specimen collection facilities and are not intended as required practices. The focus of this first version of the ISBER Best Practices is on the management of human specimen collections. It is ISBER’s intention to broaden the focus to include Best Practices surrounding other specimen types in subsequent versions. Likewise, because most of the initial contributions to this edition were from individuals based in the United States, the Practices described here primarily reflect U.S. perspectives. ISBER plans to broaden the scope of the Practices in future editions to include those from other nations in order to make this document more representative of international perspectives and experience.","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"5-48"},"PeriodicalIF":0.0,"publicationDate":"2005-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emmanuel Denou, B. Thammavongs, M. Guéguen, J. Panoff
This study was completed on an artificial microbial community (consortium) including three microorganisms of dairy interest, two mesophilic (Lactococcus lactis subsp. cremoris and Geotrichum candidum) and one thermophilic (Lactobacillus delbrueckii subsp. bulgaricus). The aim of this work was to study the stress responses of the food microbial consortium subjected to a freeze–thaw challenge (–20°C, 10 min / +25°C, 3 min) combined with physiological cryoadaptation (cryotolerance). Our results show that induction of cryotolerance by near-freezing temperature pretreatment of G. candidum and L. cremoris within the microbial community is similar to the one obtained with pure cultures. However, cryotolerance of L. bulgaricus was only induced within the consortium in milk. An interspecies cryoprotection was thus identified. This acquisition is correlated with the cell envelope extracts of G. candidum and/or the extracellular fractions of the two adapted mesophilic microorganisms. Therefore, in our experimental c...
本研究是在一个人工微生物群落(联合体)中完成的，其中包括三种乳制品微生物，两种中温微生物(乳酸乳球菌亚种)。cremoris和Geotrichum candidum)和一个嗜热菌(delbrueckii乳酸杆菌亚种)。发酵剂)保加利亚。本研究的目的是研究食品微生物群落在冻融挑战(-20°C, 10 min / +25°C, 3 min)下的应激反应以及生理低温适应(低温耐受性)。我们的研究结果表明，在微生物群落中，通过近冰点温度预处理对念珠菌和乳酸菌的低温耐受性的诱导效果与纯培养的效果相似。然而，保加利亚乳杆菌的低温耐受性仅在乳汁中诱导。因此，确定了种间冷冻保护。这种获取与G. candidum的细胞包膜提取物和/或两种适应的中温微生物的细胞外组分有关。因此，在我们的实验c…
{"title":"Interspecies Protection against Freezing Stress within a Food Microbial Community","authors":"Emmanuel Denou, B. Thammavongs, M. Guéguen, J. Panoff","doi":"10.1089/CPT.2005.3.75","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.75","url":null,"abstract":"This study was completed on an artificial microbial community (consortium) including three microorganisms of dairy interest, two mesophilic (Lactococcus lactis subsp. cremoris and Geotrichum candidum) and one thermophilic (Lactobacillus delbrueckii subsp. bulgaricus). The aim of this work was to study the stress responses of the food microbial consortium subjected to a freeze–thaw challenge (–20°C, 10 min / +25°C, 3 min) combined with physiological cryoadaptation (cryotolerance). Our results show that induction of cryotolerance by near-freezing temperature pretreatment of G. candidum and L. cremoris within the microbial community is similar to the one obtained with pure cultures. However, cryotolerance of L. bulgaricus was only induced within the consortium in milk. An interspecies cryoprotection was thus identified. This acquisition is correlated with the cell envelope extracts of G. candidum and/or the extracellular fractions of the two adapted mesophilic microorganisms. Therefore, in our experimental c...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"75-83"},"PeriodicalIF":0.0,"publicationDate":"2005-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.75","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
James C. Davis, J. Shon, David T. Wong, Syrus M. Jaffe, Jennifer McEvoy
Validity of data is inexorably linked to proper sample identification throughout the research process. Even low error rates in sample identification can have a significant, negative impact on study results. Unfortunately, most samples themselves are not tagged; instead, a label is placed on the container. The labels maybe illegibly printed, smudged, or fall off during storage. This can lead to misidentification of samples. GenVault has developed a novel backup system for labeling the biological samples themselves and not the container. For example, DNA samples are stored in a 384-well plate that contains Whatman FTA® paper and a mixture of a five or more oligonucleotide sets differing in length by 10 base pairs. This combination of oligonucleotides is referred to as GenCode and can be co-eluted along with the DNA sample, providing a permanent sample identifier. These oligonucleotides and primer pairs have been "BLASTed" against human genomic DNA sequence to ensure that they are not complementary and do no...
{"title":"A DNA-Based Biological Sample Tracking Method","authors":"James C. Davis, J. Shon, David T. Wong, Syrus M. Jaffe, Jennifer McEvoy","doi":"10.1089/CPT.2005.3.54","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.54","url":null,"abstract":"Validity of data is inexorably linked to proper sample identification throughout the research process. Even low error rates in sample identification can have a significant, negative impact on study results. Unfortunately, most samples themselves are not tagged; instead, a label is placed on the container. The labels maybe illegibly printed, smudged, or fall off during storage. This can lead to misidentification of samples. GenVault has developed a novel backup system for labeling the biological samples themselves and not the container. For example, DNA samples are stored in a 384-well plate that contains Whatman FTA® paper and a mixture of a five or more oligonucleotide sets differing in length by 10 base pairs. This combination of oligonucleotides is referred to as GenCode and can be co-eluted along with the DNA sample, providing a permanent sample identifier. These oligonucleotides and primer pairs have been \"BLASTed\" against human genomic DNA sequence to ensure that they are not complementary and do no...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"54-60"},"PeriodicalIF":0.0,"publicationDate":"2005-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.54","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Successful hypothermic storage of primary cells is now recognized as a rate-limiting step in the development of clinical applications in regenerative medicine and in research-related settings. Heart transplantation and stem cell cardiomyoplasty require hypothermic storage for successful harvest/isolation and transport to the recipient. These processes are time constrained, thereby necessitating the need to recover fully functional bioproducts following prolonged hypothermia. Despite numerous attempts, extension of the cold storage interval for myocytes while maintaining viability and function has not been realized. In this study, investigation into several strategic approaches to hypothermic preservation was evaluated in the neonatal rat ventricular cardiomyocyte model (NRVCM). Samples were assessed for survival by a panel of indicators including cellular membrane integrity, metabolic activity and spontaneous contractile function. Cultured NRVCM were held at 4°C for 24–72 h in either standard culture medi...
{"title":"Enhanced Hypothermic Storage of Neonatal Cardiomyocytes","authors":"K. Snyder, J. Baust, R. Buskirk, J. Baust","doi":"10.1089/CPT.2005.3.61","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.61","url":null,"abstract":"Successful hypothermic storage of primary cells is now recognized as a rate-limiting step in the development of clinical applications in regenerative medicine and in research-related settings. Heart transplantation and stem cell cardiomyoplasty require hypothermic storage for successful harvest/isolation and transport to the recipient. These processes are time constrained, thereby necessitating the need to recover fully functional bioproducts following prolonged hypothermia. Despite numerous attempts, extension of the cold storage interval for myocytes while maintaining viability and function has not been realized. In this study, investigation into several strategic approaches to hypothermic preservation was evaluated in the neonatal rat ventricular cardiomyocyte model (NRVCM). Samples were assessed for survival by a panel of indicators including cellular membrane integrity, metabolic activity and spontaneous contractile function. Cultured NRVCM were held at 4°C for 24–72 h in either standard culture medi...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"61-74"},"PeriodicalIF":0.0,"publicationDate":"2005-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.61","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号