The current techniques for the biopreservation of mammalian cells, such as red blood cells, human platelets, sperm cells, and stem cells, rely on hypothermic storage, which has many disadvantages including high cost of maintenance and limited transportation ability. Preservation of biomaterial in the dry state at room temperature is a novel approach to meeting the growing demand for human mammalian cells in regenerative medicine. Presently, many proteins, bacteria, pharmaceutical drugs, and foods are successfully preserved in the dried state. However, mammalian cells are desiccation sensitive and cannot be stabilized in the dried state without the use of biotechnology. Several techniques have been developed to overcome this challenge including the introduction of sugars into the cells. To date there has been encouraging, but limited, success in the development of techniques for the desiccation and dry storage of human platelets and sperm cells. This review summarizes the current state of the preservation ...
{"title":"Mammalian Cell Desiccation: Facing The Challenges","authors":"Tamir Kanias, J. Acker","doi":"10.1089/CPT.2006.9996","DOIUrl":"https://doi.org/10.1089/CPT.2006.9996","url":null,"abstract":"The current techniques for the biopreservation of mammalian cells, such as red blood cells, human platelets, sperm cells, and stem cells, rely on hypothermic storage, which has many disadvantages including high cost of maintenance and limited transportation ability. Preservation of biomaterial in the dry state at room temperature is a novel approach to meeting the growing demand for human mammalian cells in regenerative medicine. Presently, many proteins, bacteria, pharmaceutical drugs, and foods are successfully preserved in the dried state. However, mammalian cells are desiccation sensitive and cannot be stabilized in the dried state without the use of biotechnology. Several techniques have been developed to overcome this challenge including the introduction of sugars into the cells. To date there has been encouraging, but limited, success in the development of techniques for the desiccation and dry storage of human platelets and sperm cells. This review summarizes the current state of the preservation ...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"4 1","pages":"253-277"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.9996","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60915038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Enseñat‐Waser, A. Santana, Beatriz Paredes, M. Zenke, J. Reig, E. Roche
Diabetes mellitus derives from the absence of insulin hormone in the organism, usually due to the destruction of insulin-producing pancreatic β-cells either by immune attack or glucolipotoxic mechanisms. Insulin is a key hormone in controlling nutrient (glucose and fatty acids) homeostasis, as well as modulating both their uptake and metabolism by peripheral target tissues, such as skeletal muscle and adipose tissue. Insulin injection can partially mimic endogenous hormone function, although it does not avoid the appearance of secondary complications such as retinopathy, nephropathy, neuropathy, and cardiovascular disorders. Implantation of de novo insulin-producing cells in the organism could be a long-term solution in the treatment of the disease, restoring the lost function. Transplantation of highly pure isolated islets (pancreatic cell clusters where β-cells are located) from cadaveric donors is a possibility, although there are still many problems to resolve, such as immune rejection, low isolation ...
{"title":"Embryonic Stem Cell Processing in Obtaining Insulin-Producing Cells: A Technical Review","authors":"R. Enseñat‐Waser, A. Santana, Beatriz Paredes, M. Zenke, J. Reig, E. Roche","doi":"10.1089/CPT.2006.9997","DOIUrl":"https://doi.org/10.1089/CPT.2006.9997","url":null,"abstract":"Diabetes mellitus derives from the absence of insulin hormone in the organism, usually due to the destruction of insulin-producing pancreatic β-cells either by immune attack or glucolipotoxic mechanisms. Insulin is a key hormone in controlling nutrient (glucose and fatty acids) homeostasis, as well as modulating both their uptake and metabolism by peripheral target tissues, such as skeletal muscle and adipose tissue. Insulin injection can partially mimic endogenous hormone function, although it does not avoid the appearance of secondary complications such as retinopathy, nephropathy, neuropathy, and cardiovascular disorders. Implantation of de novo insulin-producing cells in the organism could be a long-term solution in the treatment of the disease, restoring the lost function. Transplantation of highly pure isolated islets (pancreatic cell clusters where β-cells are located) from cadaveric donors is a possibility, although there are still many problems to resolve, such as immune rejection, low isolation ...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"4 1","pages":"278-289"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.9997","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60915105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lower fertility of preserved stallion semen may be caused by damage to the spermatozoa or premature capacitation during storage. We investigated the use of the flavonoid, quercetin, with a standard skim milk extender for storage of stallion spermatozoa to prevent premature capacitation and acrosome reaction and to reduce the damage to spermatozoa from lipid peroxidation. Several concentrations of quercetin (0.05, 0.1, 0.2, 0.3 mM) were mixed with skim milk-glucose extender, and the antioxidant effectiveness was assessed using xanthine–xanthine oxidase challenge for 21 h. The effect of quercetin on capacitation status of sperm was assessed during a 4 h incubation at 33°C, and a storage trial at 5°C for 6 days. In addition, the ability of sperm stored in quercetin extender to undergo capacitation and acrosome reaction was assessed using heparin and A23187. Lipid peroxidation in the sperm after challenge was inhibited by any concentration of quercetin in the medium, while 0.1 and 0.3 mM quercetin were most e...
{"title":"Effect of Quercetin on Capacitation Status and Lipid Peroxidation of Stallion Spermatozoa","authors":"M. Mcniven, G. Richardson","doi":"10.1089/CPT.2006.4.169","DOIUrl":"https://doi.org/10.1089/CPT.2006.4.169","url":null,"abstract":"Lower fertility of preserved stallion semen may be caused by damage to the spermatozoa or premature capacitation during storage. We investigated the use of the flavonoid, quercetin, with a standard skim milk extender for storage of stallion spermatozoa to prevent premature capacitation and acrosome reaction and to reduce the damage to spermatozoa from lipid peroxidation. Several concentrations of quercetin (0.05, 0.1, 0.2, 0.3 mM) were mixed with skim milk-glucose extender, and the antioxidant effectiveness was assessed using xanthine–xanthine oxidase challenge for 21 h. The effect of quercetin on capacitation status of sperm was assessed during a 4 h incubation at 33°C, and a storage trial at 5°C for 6 days. In addition, the ability of sperm stored in quercetin extender to undergo capacitation and acrosome reaction was assessed using heparin and A23187. Lipid peroxidation in the sperm after challenge was inhibited by any concentration of quercetin in the medium, while 0.1 and 0.3 mM quercetin were most e...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"4 1","pages":"169-177"},"PeriodicalIF":0.0,"publicationDate":"2006-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.4.169","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60913456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Field of Cellular Cryopreservation","authors":"J. Baust","doi":"10.1089/CPT.2006.4.147","DOIUrl":"https://doi.org/10.1089/CPT.2006.4.147","url":null,"abstract":"","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"4 1","pages":"147-148"},"PeriodicalIF":0.0,"publicationDate":"2006-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.4.147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Supplementation of cryoprotectant agent (CPA) into freezing solution causes osmotic change and this change together with toxicity of CPA may damage the oocytes before freezing. The objectives of the present study were to evaluate the tolerance of mouse oocytes to osmotic changes and to compare the toxicity of the permeable CPAs. Denuded mouse oocytes were exposed to (1) 0.5 M, 1.0 M, or 2.0 M sucrose in HEPES-buffered modified human tubal fluid (mHTF), respectively, for 3 min at the room temperature; exposed to (2) either 1.0 M or 2.0 M ethylene glycol (EG), 1,2-propanediol (PROH), dimethyl sulfoxide (Me2SO) and glycerol in HEPES-buffered mHTF for 3 min at room temperature, respectively. Following exposure, the oocytes were inseminated by in vitro fertilization (IVF), and the zygotes were further cultured to assess embryonic development in vitro. Additional analyses included morphologic evaluation of meiotic spindle organization and chromosome alignment as well as aneuploidy. Exposure to 2.0 M sucrose sig...
{"title":"Effects of Osmotic Stress and Cryoprotectant Toxicity on Mouse Oocyte Fertilization and Subsequent Embryonic Development In Vitro","authors":"J. Huang, Haixiao Chen, Seang Tan, R. Chian","doi":"10.1089/CPT.2006.4.149","DOIUrl":"https://doi.org/10.1089/CPT.2006.4.149","url":null,"abstract":"Supplementation of cryoprotectant agent (CPA) into freezing solution causes osmotic change and this change together with toxicity of CPA may damage the oocytes before freezing. The objectives of the present study were to evaluate the tolerance of mouse oocytes to osmotic changes and to compare the toxicity of the permeable CPAs. Denuded mouse oocytes were exposed to (1) 0.5 M, 1.0 M, or 2.0 M sucrose in HEPES-buffered modified human tubal fluid (mHTF), respectively, for 3 min at the room temperature; exposed to (2) either 1.0 M or 2.0 M ethylene glycol (EG), 1,2-propanediol (PROH), dimethyl sulfoxide (Me2SO) and glycerol in HEPES-buffered mHTF for 3 min at room temperature, respectively. Following exposure, the oocytes were inseminated by in vitro fertilization (IVF), and the zygotes were further cultured to assess embryonic development in vitro. Additional analyses included morphologic evaluation of meiotic spindle organization and chromosome alignment as well as aneuploidy. Exposure to 2.0 M sucrose sig...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"31 1","pages":"149-160"},"PeriodicalIF":0.0,"publicationDate":"2006-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.4.149","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Trehalose was introduced into suspended primary rat hepatocytes through pathways resulting from thermally induced alterations of the cellular membrane. The hepatocytes were suspended in a diluted hepatocyte culture medium (medium:dH2O = 1:2) with 0.4 M trehalose during thermal treatments. A significant amount of cytoplasmic trehalose (0.07 M) was detected using high-performance liquid chromatography (HPLC) after heating hepatocytes to 39°C for 10 min in trehalose-supplemented medium. High cell viability (approximately 90%) was retained. The cytoplasmic trehalose concentration reached a plateau (approximately 0.16 M) after heating for 1–2 h. However, the cell viability decreased significantly after 30 min of heating (< approximately 72%). It was further found that by repetitive heating between 0°C and 39°C every 10 min for 1 h (0–39°C, 1 h), high cell viability (approximately 83%) could be maintained and a high cytoplasmic trehalose concentration (approximately 0.13 M) could be obtained. The trehalose-lade...
{"title":"Thermally Induced Introduction of Trehalose into Primary Rat Hepatocytes","authors":"Xiaoming He, Arthi A. Amin, A. Fowler, M. Toner","doi":"10.1089/CPT.2006.4.178","DOIUrl":"https://doi.org/10.1089/CPT.2006.4.178","url":null,"abstract":"Trehalose was introduced into suspended primary rat hepatocytes through pathways resulting from thermally induced alterations of the cellular membrane. The hepatocytes were suspended in a diluted hepatocyte culture medium (medium:dH2O = 1:2) with 0.4 M trehalose during thermal treatments. A significant amount of cytoplasmic trehalose (0.07 M) was detected using high-performance liquid chromatography (HPLC) after heating hepatocytes to 39°C for 10 min in trehalose-supplemented medium. High cell viability (approximately 90%) was retained. The cytoplasmic trehalose concentration reached a plateau (approximately 0.16 M) after heating for 1–2 h. However, the cell viability decreased significantly after 30 min of heating (< approximately 72%). It was further found that by repetitive heating between 0°C and 39°C every 10 min for 1 h (0–39°C, 1 h), high cell viability (approximately 83%) could be maintained and a high cytoplasmic trehalose concentration (approximately 0.13 M) could be obtained. The trehalose-lade...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"8 1","pages":"178-187"},"PeriodicalIF":0.0,"publicationDate":"2006-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.4.178","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60913606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Arcese, A. Picardi, R. Cerretti, L. Cudillo, G. Angelis, L. Franceschini, L. Felice, M. Postorino
{"title":"The Therapeutic Use of Cord Blood","authors":"W. Arcese, A. Picardi, R. Cerretti, L. Cudillo, G. Angelis, L. Franceschini, L. Felice, M. Postorino","doi":"10.1089/CPT.2006.4.161","DOIUrl":"https://doi.org/10.1089/CPT.2006.4.161","url":null,"abstract":"","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"4 1","pages":"161-168"},"PeriodicalIF":0.0,"publicationDate":"2006-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.4.161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Hassa, Firdevs Gurer Dr., A. Yildirim, Cavit Can, V. Sahinturk, B. Tekin
It is well known that an effective freezing technique is crucial for small numbers of human sperm using empty zona pellucida as straws. However, this method still has problems. The goal of this stu...
{"title":"A New Protection Solution for Freezing Small Numbers of Sperm Inside Empty Zona Pellucida: Osmangazi-Turk Solution","authors":"H. Hassa, Firdevs Gurer Dr., A. Yildirim, Cavit Can, V. Sahinturk, B. Tekin","doi":"10.1089/CPT.2006.4.199","DOIUrl":"https://doi.org/10.1089/CPT.2006.4.199","url":null,"abstract":"It is well known that an effective freezing technique is crucial for small numbers of human sperm using empty zona pellucida as straws. However, this method still has problems. The goal of this stu...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"4 1","pages":"199-208"},"PeriodicalIF":0.0,"publicationDate":"2006-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2006.4.199","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60913246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：