Sandhya S. Buchanan, M. Menze, S. Hand, D. Pyatt, J. Carpenter
Hematopoietic stem and progenitor cells (HPCs) are a heterogenic population of cells used to treat a number of human diseases. Multilineage differentiation is a required function in successful hema...
{"title":"Cryopreservation of Human Hematopoietic Stem and Progenitor Cells Loaded with Trehalose: Transient Permeabilization via the Adenosine Triphosphate-Dependent P2Z Receptor Channel","authors":"Sandhya S. Buchanan, M. Menze, S. Hand, D. Pyatt, J. Carpenter","doi":"10.1089/CPT.2005.3.212","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.212","url":null,"abstract":"Hematopoietic stem and progenitor cells (HPCs) are a heterogenic population of cells used to treat a number of human diseases. Multilineage differentiation is a required function in successful hema...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"212-222"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.212","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The design and implementation of a large-scale biologic repository is typically an exercise in converting a basic warehouse-type building into a highly sophisticated storage facility. A model for a repository with infrastructure to accommodate 100 units for subambient temperature storage is utilized. We present an outline of the design process and considerations, timelines, and a cost model. Included are guides for determining the floor space required, and recommendations for establishing engineering design features. Our model projects a 7000-square foot facility, requiring 6 to 8 months to design and build, uses 30 freezer units at start-up, with an initial cost of $1.5 to $2.5 million.
{"title":"Large-Scale Repository Design","authors":"Philip M Baird, Richard J. Frome","doi":"10.1089/CPT.2005.3.256","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.256","url":null,"abstract":"The design and implementation of a large-scale biologic repository is typically an exercise in converting a basic warehouse-type building into a highly sophisticated storage facility. A model for a repository with infrastructure to accommodate 100 units for subambient temperature storage is utilized. We present an outline of the design process and considerations, timelines, and a cost model. Included are guides for determining the floor space required, and recommendations for establishing engineering design features. Our model projects a 7000-square foot facility, requiring 6 to 8 months to design and build, uses 30 freezer units at start-up, with an initial cost of $1.5 to $2.5 million.","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"256-266"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.256","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vitrification is the solidification of a solution without crystallization, caused by an increase of viscosity. It results from a balance between the highest realizable cooling rates and the highest concentrations of cryoprotectants (CPA) not toxic for the cells for the period of exposure. To vitrify with reduced CPA concentrations the cooling rates have to be increased. The objective of our study was to verify the thermal feasibility of a vitrification process using a computational fluid dynamics (CFD) model. The first step in our stepwise approach to our project is focused on an ultrarapid freezing process in absence of cryoprotectants. The main parameters which influence the cooldown and increased cooling rate inside the biologic material (e.g., the thermophysical parameters, the size and the shape of the specimen) have been investigated. In our test cases the living material is considered frozen by direct contact with a surface at cryogenic temperature. Both numerical values and false color images of t...
{"title":"Computational Fluid Dynamics Analysis of Cell Cooling Process","authors":"R. Marchesi, Manuela Maffè, Matteo Moraschi","doi":"10.1089/CPT.2005.3.229","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.229","url":null,"abstract":"Vitrification is the solidification of a solution without crystallization, caused by an increase of viscosity. It results from a balance between the highest realizable cooling rates and the highest concentrations of cryoprotectants (CPA) not toxic for the cells for the period of exposure. To vitrify with reduced CPA concentrations the cooling rates have to be increased. The objective of our study was to verify the thermal feasibility of a vitrification process using a computational fluid dynamics (CFD) model. The first step in our stepwise approach to our project is focused on an ultrarapid freezing process in absence of cryoprotectants. The main parameters which influence the cooldown and increased cooling rate inside the biologic material (e.g., the thermophysical parameters, the size and the shape of the specimen) have been investigated. In our test cases the living material is considered frozen by direct contact with a surface at cryogenic temperature. Both numerical values and false color images of t...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"223-228"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.229","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of this study was to test whether cyclic tetrasaccharide could prevent cataract formation in isolated porcine lenses in vitro. Porcine eyes were cut at the midperiphery with a razor blade a...
本研究旨在探讨环四糖对猪离体晶状体白内障的预防作用。用剃须刀片在猪眼睛的中间边缘切开。
{"title":"Cyclic tetrasaccharide delays cataract formation in the lens in vitro","authors":"T. Matsuo","doi":"10.1089/CPT.2005.3.238","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.238","url":null,"abstract":"The aim of this study was to test whether cyclic tetrasaccharide could prevent cataract formation in isolated porcine lenses in vitro. Porcine eyes were cut at the midperiphery with a razor blade a...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"108 1","pages":"238-243"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.238","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Liquid cooled boar semen is highly susceptible to lipid peroxidation and capacitation in vitro during storage, which results in reduced fertility and litter sizes following artificial insemination (AI). Antioxidants can delay lipid peroxidation in vitro and therefore potentially extend the lifespan of stored semen. Lowbush blueberries (Vaccinium angustifolium Aiton) and their leaves contain high antioxidant capacity, making them potential candidates for preserving liquid cooled boar semen during storage. This study compared the reversible ability of blueberry extract, leaf extract, and the antioxidant quercetin to prevent capacitation of extended, cooled boar semen during storage for 1 week. The results indicated that blueberry leaf extract and quercetin were equally capable of delaying capacitation for 1 week and still allow capacitation to occur in vitro following incubation at 39°C. Blueberry extract also delayed capacitation but only during storage for 5 days; its effects were also reversible. Blueber...
{"title":"The Effect of Blueberry Extracts and Quercetin on Capacitation Status of Stored Boar Sperm","authors":"N. Desroches, M. Mcniven, K. Foote, G. Richardson","doi":"10.1089/CPT.2005.3.165","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.165","url":null,"abstract":"Liquid cooled boar semen is highly susceptible to lipid peroxidation and capacitation in vitro during storage, which results in reduced fertility and litter sizes following artificial insemination (AI). Antioxidants can delay lipid peroxidation in vitro and therefore potentially extend the lifespan of stored semen. Lowbush blueberries (Vaccinium angustifolium Aiton) and their leaves contain high antioxidant capacity, making them potential candidates for preserving liquid cooled boar semen during storage. This study compared the reversible ability of blueberry extract, leaf extract, and the antioxidant quercetin to prevent capacitation of extended, cooled boar semen during storage for 1 week. The results indicated that blueberry leaf extract and quercetin were equally capable of delaying capacitation for 1 week and still allow capacitation to occur in vitro following incubation at 39°C. Blueberry extract also delayed capacitation but only during storage for 5 days; its effects were also reversible. Blueber...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"6 1","pages":"165-168"},"PeriodicalIF":0.0,"publicationDate":"2005-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.165","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Cherkashina, O. Semenchenko, V. Grischuk, B. Fuller, A. Petrenko
The search for effective methods of long-term liver hypothermic preservation is one of the most important problems in hepatic transplantation. Biologically active substances contained in fetal tissues may be potent stimulators and regulators of metabolic processes, which can support liver during hypothermic storage and after normothermic reperfusion (HS/NR). The aim of this work was to investigate possible influences of fetal-specific factors (FSF) on liver pro-oxidant/antioxidant status, which frequently becomes disturbed during HS/NR in a rat model. As a source of FSF, human fetal tissue cytosolic fraction was used. At 4 h before liver isolation, FSF or Hank's solution were introduced into rat femoral vein (0.3 mL/100 g of body weight). Livers were subjected to HS at 4°C in sucrose-based solution (SBS) for 1 or 24 h, followed by NR for 60 min in vitro. After 1 and 24 h HS, FSF supplementation caused the normalization of hepatic thiobarbituric acid-reactive substances (TBARS) and their accumulation durin...
{"title":"Supplementation with Fetal-Specific Factors Ameliorates Oxidative Liver Damage During Hypothermic Storage and Reperfusion in a Rat Model","authors":"D. Cherkashina, O. Semenchenko, V. Grischuk, B. Fuller, A. Petrenko","doi":"10.1089/CPT.2005.3.201","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.201","url":null,"abstract":"The search for effective methods of long-term liver hypothermic preservation is one of the most important problems in hepatic transplantation. Biologically active substances contained in fetal tissues may be potent stimulators and regulators of metabolic processes, which can support liver during hypothermic storage and after normothermic reperfusion (HS/NR). The aim of this work was to investigate possible influences of fetal-specific factors (FSF) on liver pro-oxidant/antioxidant status, which frequently becomes disturbed during HS/NR in a rat model. As a source of FSF, human fetal tissue cytosolic fraction was used. At 4 h before liver isolation, FSF or Hank's solution were introduced into rat femoral vein (0.3 mL/100 g of body weight). Livers were subjected to HS at 4°C in sucrose-based solution (SBS) for 1 or 24 h, followed by NR for 60 min in vitro. After 1 and 24 h HS, FSF supplementation caused the normalization of hepatic thiobarbituric acid-reactive substances (TBARS) and their accumulation durin...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"201-209"},"PeriodicalIF":0.0,"publicationDate":"2005-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Lecchi, S. Giovanelli, M. Polese, Laura Musacchio, F. Garcea, I. Ratti, M. Brasca, P. Rebulla
In spite of abundant literature on cryobiology, relatively limited published information is available on specific features of the facilities where biological materials are stored. In this article the repository facility of a large cord blood bank is described. The 122 sq m biostorage area, which is part of the 226 sq m Milano Cord Blood Bank, is located in the basement of a hospital blood transfusion service. The repository area was designed to contain 35 liquid nitrogen (LN) tanks equipped with an automated refilling system. It was constructed according to Italian and French norms and guidelines. The current setting includes the following elements: an outdoor 6000 L reservoir connected to the area through a LN vacuum line, 30 LN tanks, a ventilation system interfaced to six oxygen sensors and a management system based on a programmable logic controller (PLC) linked to commercial proprietary software for continuous and real time monitoring of LN tanks and of the safety parameters of the repository area. T...
{"title":"Repository facility: The Milano Cord Blood Bank","authors":"L. Lecchi, S. Giovanelli, M. Polese, Laura Musacchio, F. Garcea, I. Ratti, M. Brasca, P. Rebulla","doi":"10.1089/CPT.2005.3.141","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.141","url":null,"abstract":"In spite of abundant literature on cryobiology, relatively limited published information is available on specific features of the facilities where biological materials are stored. In this article the repository facility of a large cord blood bank is described. The 122 sq m biostorage area, which is part of the 226 sq m Milano Cord Blood Bank, is located in the basement of a hospital blood transfusion service. The repository area was designed to contain 35 liquid nitrogen (LN) tanks equipped with an automated refilling system. It was constructed according to Italian and French norms and guidelines. The current setting includes the following elements: an outdoor 6000 L reservoir connected to the area through a LN vacuum line, 30 LN tanks, a ventilation system interfaced to six oxygen sensors and a management system based on a programmable logic controller (PLC) linked to commercial proprietary software for continuous and real time monitoring of LN tanks and of the safety parameters of the repository area. T...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"141-147"},"PeriodicalIF":0.0,"publicationDate":"2005-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stabilization of living cells during dehydration could facilitate the storage and transport of nucleated cells. HeLa cells, known for their resilience and insensitivity to environmental stresses, provide a useful platform for the investigation of resistance to dehydration-induced damage. HeLa cells from two sources [the American Type Culture Collection (ATCC) and the European Collection of Cell Cultures (ECCC)] were compared with regard to their survival following vacuum-drying and the dependence of viability on drying temperature. Interestingly, a substantial difference in dehydration resistance appeared between the two cell lines dried after they were loaded with trehalose. At all temperatures tested, the ATCC cell line showed higher viability than the ECCC cell line. For both lines, viabilities decreased at elevated drying temperatures, but even when the cells were dried at 45°C, the ATCC line survived well compared to the ECCC line (27% vs. 0%, respectively, when dried to 0.3 g H2O/g dry weight). The ...
脱水过程中活细胞的稳定有助于有核细胞的储存和运输。HeLa细胞以其弹性和对环境胁迫的不敏感而闻名，为研究对脱水诱导损伤的抗性提供了一个有用的平台。我们比较了两种来源的HeLa细胞(American Type Culture Collection (ATCC)和European Collection of Cell Cultures (ECCC))在真空干燥后的存活率和对干燥温度的依赖性。有趣的是，在加载海藻糖后干燥的两种细胞系之间出现了显著的脱水抗性差异。在所有温度下，ATCC细胞系的生存能力均高于ECCC细胞系。两种细胞系的存活率在较高的干燥温度下都有所下降，但即使在45℃下干燥，ATCC细胞系的存活率也比ECCC细胞系好(当干燥至0.3 g H2O/g干重时，存活率分别为27%和0%)。…
{"title":"Resistance to Dehydration Damage in HeLa Cells Correlates with the Presence of Endogenous Heat Shock Proteins","authors":"R. Ravindran, F. Tablin, J. Crowe, A. E. Oliver","doi":"10.1089/CPT.2005.3.155","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.155","url":null,"abstract":"Stabilization of living cells during dehydration could facilitate the storage and transport of nucleated cells. HeLa cells, known for their resilience and insensitivity to environmental stresses, provide a useful platform for the investigation of resistance to dehydration-induced damage. HeLa cells from two sources [the American Type Culture Collection (ATCC) and the European Collection of Cell Cultures (ECCC)] were compared with regard to their survival following vacuum-drying and the dependence of viability on drying temperature. Interestingly, a substantial difference in dehydration resistance appeared between the two cell lines dried after they were loaded with trehalose. At all temperatures tested, the ATCC cell line showed higher viability than the ECCC cell line. For both lines, viabilities decreased at elevated drying temperatures, but even when the cells were dried at 45°C, the ATCC line survived well compared to the ECCC line (27% vs. 0%, respectively, when dried to 0.3 g H2O/g dry weight). The ...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"52 1","pages":"155-164"},"PeriodicalIF":0.0,"publicationDate":"2005-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Nagendran, S. Meyer, D. Webb, B. Sykes, J. Lakey, D. Ross
Decellularization of allograft heart valves has been proposed as a method to reduce the alloreactive immune response, which limits the durability of these tissues. Rat models are essential for preliminary studies of the immunogenicity of decellularized tissue, but concerns have arisen about the toxic effects of Triton X-100 commonly used in a number of decellularization protocols. The purpose of this study was to determine the optimal washout conditions of Triton X-100 following decellularization. Sprague-Dawley rat aortic valves were decellularized with a combination of hypotonic and hypertonic buffers, protease inhibitors, and gentle detergents (0.5% Triton X-100) followed by a washout in phosphate buffered saline (PBS) for variable time periods. Decellularized valves were allowed to equilibrate in ddH2O and residual levels of Triton X-100 were determined using proton nuclear magnetic resonance spectroscopy (1H-NMR). The majority of Triton X-100 was removed with a PBS washout time of 4 hours, yielding a...
{"title":"1H NMR Assessment of Safe Triton X-100 Levels in Decellularized Rat Aortic Valve Tissue","authors":"J. Nagendran, S. Meyer, D. Webb, B. Sykes, J. Lakey, D. Ross","doi":"10.1089/CPT.2005.3.148","DOIUrl":"https://doi.org/10.1089/CPT.2005.3.148","url":null,"abstract":"Decellularization of allograft heart valves has been proposed as a method to reduce the alloreactive immune response, which limits the durability of these tissues. Rat models are essential for preliminary studies of the immunogenicity of decellularized tissue, but concerns have arisen about the toxic effects of Triton X-100 commonly used in a number of decellularization protocols. The purpose of this study was to determine the optimal washout conditions of Triton X-100 following decellularization. Sprague-Dawley rat aortic valves were decellularized with a combination of hypotonic and hypertonic buffers, protease inhibitors, and gentle detergents (0.5% Triton X-100) followed by a washout in phosphate buffered saline (PBS) for variable time periods. Decellularized valves were allowed to equilibrate in ddH2O and residual levels of Triton X-100 were determined using proton nuclear magnetic resonance spectroscopy (1H-NMR). The majority of Triton X-100 was removed with a PBS washout time of 4 hours, yielding a...","PeriodicalId":51233,"journal":{"name":"Cell Preservation Technology","volume":"3 1","pages":"148-155"},"PeriodicalIF":0.0,"publicationDate":"2005-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/CPT.2005.3.148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60912411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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