Leichang Huang, Cong He Peng, Shanhua Bi, Shan Shan Cong, Sai Fei Li, Lin Wang, Yu Li, Xiaoyu Jia
Coastal zone has become an advantageous resources and research hotspot needed for human development due to its diverse biology, abundant resources, beautiful landscape and remarkable location. Faced with ecological problems such as sketchy utilization, excessive intensity, environment and landscape; The research is scattered and partial, lacking of functional positioning and the management is chaotic. The coastal zone around Bohai Bay is a typical representative of the coastal belt landscape in the north temperate zone of China. It also faces the same problem, and the topic selection is urgent and necessary. Based on the integrity of ecosystem, this paper analyzes the key problems and builds an ecological control technology system for the protection and utilization of the Coastal Zone landscape around the Bohai Bay based on the macro, meso and micro scales: that is, the macro ecological control technology based on the planning of shoreline national parks, the meso land and sea overall planning and comprehensive elastic management ecological control technology based on artificial ecosystem ecology and social ecosystem, and the ecological barrier, functional zoning, diagnostic evaluation Micro integrated ecological control technology such as ecological engineering. Lay a foundation for the protection and utilization of coastal zone landscape.
{"title":"Study on the Construction of Ecological Control Technology Model Based on the Protection and Utilization of Coastal Landscape in the Bohai Bay Rim","authors":"Leichang Huang, Cong He Peng, Shanhua Bi, Shan Shan Cong, Sai Fei Li, Lin Wang, Yu Li, Xiaoyu Jia","doi":"10.4028/p-vkc3ok","DOIUrl":"https://doi.org/10.4028/p-vkc3ok","url":null,"abstract":"Coastal zone has become an advantageous resources and research hotspot needed for human development due to its diverse biology, abundant resources, beautiful landscape and remarkable location. Faced with ecological problems such as sketchy utilization, excessive intensity, environment and landscape; The research is scattered and partial, lacking of functional positioning and the management is chaotic. The coastal zone around Bohai Bay is a typical representative of the coastal belt landscape in the north temperate zone of China. It also faces the same problem, and the topic selection is urgent and necessary.\u0000Based on the integrity of ecosystem, this paper analyzes the key problems and builds an ecological control technology system for the protection and utilization of the Coastal Zone landscape around the Bohai Bay based on the macro, meso and micro scales: that is, the macro ecological control technology based on the planning of shoreline national parks, the meso land and sea overall planning and comprehensive elastic management ecological control technology based on artificial ecosystem ecology and social ecosystem, and the ecological barrier, functional zoning, diagnostic evaluation Micro integrated ecological control technology such as ecological engineering. Lay a foundation for the protection and utilization of coastal zone landscape.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141648661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dan Yong Wang, Hua Zhen Wei, Shu Hu Li, Wen Fang Xing
Due to the complex compression failure process of fiber composite materials, especially the direct bearing situation of the composite material end face, it is quite difficult to accurately predict it. At present, the design of composite material compression performance mainly considers a large safety factor, and a compression failure assessment method that can meet the needs of reliable engineering applications is still a challenge and a key issue that the engineering community urgently needs to solve. This article reviews the widely studied shear type buckling model, extensional type buckling model, matrix strain failure theoretical model, matrix shear failure theoretical model, interlayer shear failure theoretical model, elastic micro buckling model, plastic micro buckling model, three parameter model, fiber microbuckling finite element model and ply mesobuckling finite element model of fiber reinforced composite materials that have been publicly published in recent years, which were analyzed and summarized, providing reference for further in-depth research in the future.
{"title":"Compression Failure Model of Fiber Reinforced Composite Materials","authors":"Dan Yong Wang, Hua Zhen Wei, Shu Hu Li, Wen Fang Xing","doi":"10.4028/p-n7savj","DOIUrl":"https://doi.org/10.4028/p-n7savj","url":null,"abstract":"Due to the complex compression failure process of fiber composite materials, especially the direct bearing situation of the composite material end face, it is quite difficult to accurately predict it. At present, the design of composite material compression performance mainly considers a large safety factor, and a compression failure assessment method that can meet the needs of reliable engineering applications is still a challenge and a key issue that the engineering community urgently needs to solve. This article reviews the widely studied shear type buckling model, extensional type buckling model, matrix strain failure theoretical model, matrix shear failure theoretical model, interlayer shear failure theoretical model, elastic micro buckling model, plastic micro buckling model, three parameter model, fiber microbuckling finite element model and ply mesobuckling finite element model of fiber reinforced composite materials that have been publicly published in recent years, which were analyzed and summarized, providing reference for further in-depth research in the future.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141649180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hai Yun Zhang, Dan Yong Wang, Shu Hu Li, Hua Zhen Wei, Jing An, Wen Fang Xing
PBO fibers are prone to large deformation under high-speed impact loads, which is conducive to energy absorption through fiber tensile deformation and fracture. This article establishes a molecular scale model of PBO microfibers, which can deeply understand the phenomenon and characteristics of damage to PBO fiber materials through molecular dynamics optimization. It also theoretically predicts the structure and physical properties of such materials, reveals their failure mechanisms, and provides technical support for the research and development of military materials.
{"title":"Research on Mechanical Properties of PBO Microfibers Based on Molecular Scale Dynamics","authors":"Hai Yun Zhang, Dan Yong Wang, Shu Hu Li, Hua Zhen Wei, Jing An, Wen Fang Xing","doi":"10.4028/p-m9xv4g","DOIUrl":"https://doi.org/10.4028/p-m9xv4g","url":null,"abstract":"PBO fibers are prone to large deformation under high-speed impact loads, which is conducive to energy absorption through fiber tensile deformation and fracture. This article establishes a molecular scale model of PBO microfibers, which can deeply understand the phenomenon and characteristics of damage to PBO fiber materials through molecular dynamics optimization. It also theoretically predicts the structure and physical properties of such materials, reveals their failure mechanisms, and provides technical support for the research and development of military materials.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141647308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hua Zhen Wei, Dan Yong Wang, Shu Hu Li, Wen Fang Xing
The microstructure of carbon fibers with different properties also varies. Even with considerable mechanical properties, the microstructure characteristics of carbon fibers produced by different manufacturers or processes are different. Although current characterization testing methods can provide a fundamental analysis of the relationship between the microstructure and mechanical properties of carbon fibers, there is still no systematic theory on how to control the evolution of their microstructure during the production process of carbon fibers and develop high-performance and high-quality fibers. There is still a certain gap in the performance of carbon fibers in China compared to foreign countries. Therefore, this article analyzes and summarizes various publicly published models of carbon fiber microstructure in recent years, providing reference for establishing a recognized analysis model that can predict the mechanical properties of carbon fibers in engineering, providing effective direction for improving carbon fiber manufacturing processes, and providing technical support for developing high-performance and high-quality carbon fibers.
{"title":"Research on Microscopic Morphology Analysis Model of Carbon Fiber","authors":"Hua Zhen Wei, Dan Yong Wang, Shu Hu Li, Wen Fang Xing","doi":"10.4028/p-r6rxhr","DOIUrl":"https://doi.org/10.4028/p-r6rxhr","url":null,"abstract":"The microstructure of carbon fibers with different properties also varies. Even with considerable mechanical properties, the microstructure characteristics of carbon fibers produced by different manufacturers or processes are different. Although current characterization testing methods can provide a fundamental analysis of the relationship between the microstructure and mechanical properties of carbon fibers, there is still no systematic theory on how to control the evolution of their microstructure during the production process of carbon fibers and develop high-performance and high-quality fibers. There is still a certain gap in the performance of carbon fibers in China compared to foreign countries. Therefore, this article analyzes and summarizes various publicly published models of carbon fiber microstructure in recent years, providing reference for establishing a recognized analysis model that can predict the mechanical properties of carbon fibers in engineering, providing effective direction for improving carbon fiber manufacturing processes, and providing technical support for developing high-performance and high-quality carbon fibers.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141648747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
For deep overburden embankment base, erosion-type internal tube surge is a key problem affecting the stable operation of dams. To study the fine view mechanism of erosion type tube surge in deep overburden, this paper integrates transparent soil technology, planar laser-induced fluorescence imaging technology, firstly, through self-developed transparent soil and visualization model test platform, carry out saturated transparent soil test research, so as to obtain the two-dimensional slice collection of transparent soil samples; then with the help of Avzio software to its three-dimensional reconstruction, split solid particles and pore liquid, the pore network model is generated in the three-dimensional model with Avzio software to obtain the pore-related parameters and throat-related parameters, and plot the pore equivalent diameter and throat diameter distribution; finally, the critical fine particle diameter distribution curve is predicted by combining the previous statistical throat diameter distribution curve, and then the internal stability of the soil is evaluated, and the accurate throat distribution curve obtained predicts the fine particle diameter distribution curve. The method is proposed to evaluate the internal stability of soil by predicting the fine particle size distribution curve from the obtained accurate throat distribution curve.
{"title":"Based on Transparent Soil Test to Carry out Research on the Soil Stability Discrimination under the Suffusion","authors":"Yu Kun Liu, Jia Wei Wang","doi":"10.4028/p-y6tymh","DOIUrl":"https://doi.org/10.4028/p-y6tymh","url":null,"abstract":"For deep overburden embankment base, erosion-type internal tube surge is a key problem affecting the stable operation of dams. To study the fine view mechanism of erosion type tube surge in deep overburden, this paper integrates transparent soil technology, planar laser-induced fluorescence imaging technology, firstly, through self-developed transparent soil and visualization model test platform, carry out saturated transparent soil test research, so as to obtain the two-dimensional slice collection of transparent soil samples; then with the help of Avzio software to its three-dimensional reconstruction, split solid particles and pore liquid, the pore network model is generated in the three-dimensional model with Avzio software to obtain the pore-related parameters and throat-related parameters, and plot the pore equivalent diameter and throat diameter distribution; finally, the critical fine particle diameter distribution curve is predicted by combining the previous statistical throat diameter distribution curve, and then the internal stability of the soil is evaluated, and the accurate throat distribution curve obtained predicts the fine particle diameter distribution curve. The method is proposed to evaluate the internal stability of soil by predicting the fine particle size distribution curve from the obtained accurate throat distribution curve.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141646105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Windproof measures are the focus of attention in the process of photovoltaic engineering construction. Windproof measures for photovoltaic modules and supports have a mature design method and acceptance standard. However, the windproof measures of the cable slot box are often ignored, rarely mentioned in the procedures and specifications, there are possible accidents caused by, the risk of accidents can not be ignored. Based on the actual engineering examples, the causes of typhoon accident of cable trough box are analyzed and the windproof measures of cable trough box are calculated quantitatively.
{"title":"Calculation and Analysis of Windproof Measures of Cable Trough Box of Roof Photovoltaic Engineering","authors":"Cheng Shi, Liang Xiao, Yi Zhang","doi":"10.4028/p-xz9n8j","DOIUrl":"https://doi.org/10.4028/p-xz9n8j","url":null,"abstract":"Windproof measures are the focus of attention in the process of photovoltaic engineering construction. Windproof measures for photovoltaic modules and supports have a mature design method and acceptance standard. However, the windproof measures of the cable slot box are often ignored, rarely mentioned in the procedures and specifications, there are possible accidents caused by, the risk of accidents can not be ignored. Based on the actual engineering examples, the causes of typhoon accident of cable trough box are analyzed and the windproof measures of cable trough box are calculated quantitatively.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141646782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Rahmawati, S. Nurkhamidah, Annisa Alifia Rahmah, M. A. Rifai
Indonesia is the fifth ranked country with plastic waste that is not managed properly. Over time, plastic breaks down into microplastics (MPs) less than 5 mm in diameter, which in water can cause damage. One method of removing MPs that is considered efficient is MPs microfiltration using membrane technology. To obtain an adequate membrane in removing MPs particles, it is necessary to modify the membrane both in the material and the membrane manufacturing process itself. So this study aims to study the effect of immersion time in the manufacturing process on the characteristics and performance of microfiltration membranes to remove MPs in water. In this study, the membrane will be made using the phase inversion method with a flat sheet membrane finish. The membrane is made using Cellulose Acetate (CA) polymer with n-Methyl Pyrolidone (NMP) solvent and aquadest as its non-solvent. The selected variable is the membrane immersion time for 10; 17,5; and 25 minutes and operating pressure at membrane performance test 0,3; 0,5; and 0,7 bar. Furthermore, the resulting membrane will be analyzed for its characteristics through porosity and water content analysis, contact angle analysis, and SEM analysis. In addition, membrane performance tests are carried out to determine the ability of membranes when separating microplastics in water. CA/NMP flat sheet membrane fabrication produces white membrane flatsheet. The results of the experiments that have been carried out, obtained CA/NMP (15:85) membrane with a variable immersion time of 25 minutes has the best characteristics and performance. The membrane is white, has a thickness of 126μm, and is hydrophilic. The membrane also has a supporting layer with a finger-shaped pore structure and sponge. In addition, CA/NMP (15:85) membranes have a %microplastic rejection value in water reaching 99%.
{"title":"Fabrication and Characterization of Cellulose Acetat / N-Methyl Pyrollidon Membrane for Microplastics Separation in Water","authors":"Y. Rahmawati, S. Nurkhamidah, Annisa Alifia Rahmah, M. A. Rifai","doi":"10.4028/p-xiyvv5","DOIUrl":"https://doi.org/10.4028/p-xiyvv5","url":null,"abstract":"Indonesia is the fifth ranked country with plastic waste that is not managed properly. Over time, plastic breaks down into microplastics (MPs) less than 5 mm in diameter, which in water can cause damage. One method of removing MPs that is considered efficient is MPs microfiltration using membrane technology. To obtain an adequate membrane in removing MPs particles, it is necessary to modify the membrane both in the material and the membrane manufacturing process itself. So this study aims to study the effect of immersion time in the manufacturing process on the characteristics and performance of microfiltration membranes to remove MPs in water. In this study, the membrane will be made using the phase inversion method with a flat sheet membrane finish. The membrane is made using Cellulose Acetate (CA) polymer with n-Methyl Pyrolidone (NMP) solvent and aquadest as its non-solvent. The selected variable is the membrane immersion time for 10; 17,5; and 25 minutes and operating pressure at membrane performance test 0,3; 0,5; and 0,7 bar. Furthermore, the resulting membrane will be analyzed for its characteristics through porosity and water content analysis, contact angle analysis, and SEM analysis. In addition, membrane performance tests are carried out to determine the ability of membranes when separating microplastics in water. CA/NMP flat sheet membrane fabrication produces white membrane flatsheet. The results of the experiments that have been carried out, obtained CA/NMP (15:85) membrane with a variable immersion time of 25 minutes has the best characteristics and performance. The membrane is white, has a thickness of 126μm, and is hydrophilic. The membrane also has a supporting layer with a finger-shaped pore structure and sponge. In addition, CA/NMP (15:85) membranes have a %microplastic rejection value in water reaching 99%.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141675270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The 5th International Conference on Chemistry and Material Sciences (IC2MS)","authors":"Lukman Hakim, E. Iftitah","doi":"10.4028/b-fzb78x","DOIUrl":"https://doi.org/10.4028/b-fzb78x","url":null,"abstract":"","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141673939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dyah Ayu Nastiti, Anisya Tri Kurniawati Anwar, Achmad Sjaifullah, Busroni Busroni, Muhammad Reza
Shrimp waste contains a high content of chitin which is potential to be used as a chitosan’s precursor. Synthesis of chitosan is usually done by deproteination, demineralization, and deacetylation process. Deacetylation of chitin from shrimp waste isolated by autolysis, has been a few reported before. The chemicals involved in autolysis are less harmful and easier to treat before their disposal. Hence, this paper investigates the effect of base type and concentration on the degree of deacetylation of chitosan from chitin isolated by autolysis. Autolysis was carried out by an incubation at pH 2 using sulfuric acid for 10 d. Demineralization was performed by immersion in hydrochloric acid pH 1 for 24 h. The deacetylation of chitin was carried out at 120 °C for 120 min using two different bases, which are NaOH and KOH, respectively. The determination of chitosan’s degree of deacetylation (DD) was carried out using a semi-quantitative method from IR spectra. The use of KOH resulted in the obtained DD of less than 20%, while the NaOH usage produced around 50% of DD. Then, the NaOH was chosen and studied further to obtain a suitable DD for film applications, which is 40 – 99%. The deacetylation of chitosan was carried out by varying NaOH concentration from 60 to 70% (w/v). High concentration of NaOH tends to increase chitosan’s DD and slightly decrease the yield. The optimum concentration of NaOH was obtained at 70% (w/v) producing DD of 53.50±0.83% and yield of 47.66±0.28%. Chitosan synthesized using 70% concentration of NaOH produced a relatively homogeneous thin film. Polyaniline was then introduced to the film to obtain a prototype of smart packaging. This smart packaging was able to detect the pH changes proven by the change of its color.
{"title":"Preparation of Chitosan Film for Smart Packaging: The Effects of Base on Deacetylation Process","authors":"Dyah Ayu Nastiti, Anisya Tri Kurniawati Anwar, Achmad Sjaifullah, Busroni Busroni, Muhammad Reza","doi":"10.4028/p-pua9hz","DOIUrl":"https://doi.org/10.4028/p-pua9hz","url":null,"abstract":"Shrimp waste contains a high content of chitin which is potential to be used as a chitosan’s precursor. Synthesis of chitosan is usually done by deproteination, demineralization, and deacetylation process. Deacetylation of chitin from shrimp waste isolated by autolysis, has been a few reported before. The chemicals involved in autolysis are less harmful and easier to treat before their disposal. Hence, this paper investigates the effect of base type and concentration on the degree of deacetylation of chitosan from chitin isolated by autolysis. Autolysis was carried out by an incubation at pH 2 using sulfuric acid for 10 d. Demineralization was performed by immersion in hydrochloric acid pH 1 for 24 h. The deacetylation of chitin was carried out at 120 °C for 120 min using two different bases, which are NaOH and KOH, respectively. The determination of chitosan’s degree of deacetylation (DD) was carried out using a semi-quantitative method from IR spectra. The use of KOH resulted in the obtained DD of less than 20%, while the NaOH usage produced around 50% of DD. Then, the NaOH was chosen and studied further to obtain a suitable DD for film applications, which is 40 – 99%. The deacetylation of chitosan was carried out by varying NaOH concentration from 60 to 70% (w/v). High concentration of NaOH tends to increase chitosan’s DD and slightly decrease the yield. The optimum concentration of NaOH was obtained at 70% (w/v) producing DD of 53.50±0.83% and yield of 47.66±0.28%. Chitosan synthesized using 70% concentration of NaOH produced a relatively homogeneous thin film. Polyaniline was then introduced to the film to obtain a prototype of smart packaging. This smart packaging was able to detect the pH changes proven by the change of its color.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141677034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mochamad Hendy Fathur Rahman, E. Iftitah, Anna Roosdiana, Selvia Eka Wulandari
Isoeugenol (2-methoxy-4-[(E)-prop-1-enyl] phenol) is a compound resulting from the isomerization of eugenol contained in clove oil (Syzygium aromaticum). Isoeugenol can be used as a precursor for vanillin biosynthesis through the biotransformation pathway. In this research, the biotransformation of isoeugenol was carried out using Pseudomonas aeruginosa as an enzyme- biocatalyst agent. The parameters used in this research include the effect of substrate concentrations of 0.5; 1; 1.5; and 2% v/v, incubation times of 24; 48; 72; and 96 hours, as well as extracting solvents with ethyl acetate and chloroform. The determination of substrate concentration was carried out at an incubation time of 24 hours, and then the characterization results with the best product concentration were used to determine the incubation time. The results of qualitative identification and characterization show that with increasing the substrate concentration, it can cause decreasing the target biotransformation results. The 1% concentration treatment with the most concentrated magenta-purple color intensity from the Schiff reagent test and the most concentrated intensity of the TLC stain has more potential to produce vanillin products with an area of 0.51% (ethyl acetate) and 0.36% (chloroform), as well as vanillyl methyl ketone with an area of 1.38% (ethyl acetate) and 4.91% (chloroform). On the other hand, increasing the incubation time can reduce the target biotransformation product. The 72 hours incubation time treatment produced vanillin 0.19% (ethyl acetate) and 0.74 (chloroform), as well as vanillyl methyl ketone 1.96% (ethyl acetate), and no vanillyl methyl ketone was produced in the chloroform solvent. In the biotransformation carried out, the substrate concentration was 1% and the incubation time was 24 hours in the chloroform extracting solvent, which became a more potential condition to produce the target biotransformation product with a substrate conversion of 5.27%, which was selective for vanillyl methyl ketone at 93.17% and vanillin at 6.83%.
{"title":"Biotransformation of Isoeugenol Into Vanillin and its Derivatives Using Pseudomonas aeruginosa as an Enzyme Biocatalyst Agent: Effect of Substrate Concentration and Incubation Time","authors":"Mochamad Hendy Fathur Rahman, E. Iftitah, Anna Roosdiana, Selvia Eka Wulandari","doi":"10.4028/p-liv3pp","DOIUrl":"https://doi.org/10.4028/p-liv3pp","url":null,"abstract":"Isoeugenol (2-methoxy-4-[(E)-prop-1-enyl] phenol) is a compound resulting from the isomerization of eugenol contained in clove oil (Syzygium aromaticum). Isoeugenol can be used as a precursor for vanillin biosynthesis through the biotransformation pathway. In this research, the biotransformation of isoeugenol was carried out using Pseudomonas aeruginosa as an enzyme- biocatalyst agent. The parameters used in this research include the effect of substrate concentrations of 0.5; 1; 1.5; and 2% v/v, incubation times of 24; 48; 72; and 96 hours, as well as extracting solvents with ethyl acetate and chloroform. The determination of substrate concentration was carried out at an incubation time of 24 hours, and then the characterization results with the best product concentration were used to determine the incubation time. The results of qualitative identification and characterization show that with increasing the substrate concentration, it can cause decreasing the target biotransformation results. The 1% concentration treatment with the most concentrated magenta-purple color intensity from the Schiff reagent test and the most concentrated intensity of the TLC stain has more potential to produce vanillin products with an area of 0.51% (ethyl acetate) and 0.36% (chloroform), as well as vanillyl methyl ketone with an area of 1.38% (ethyl acetate) and 4.91% (chloroform). On the other hand, increasing the incubation time can reduce the target biotransformation product. The 72 hours incubation time treatment produced vanillin 0.19% (ethyl acetate) and 0.74 (chloroform), as well as vanillyl methyl ketone 1.96% (ethyl acetate), and no vanillyl methyl ketone was produced in the chloroform solvent. In the biotransformation carried out, the substrate concentration was 1% and the incubation time was 24 hours in the chloroform extracting solvent, which became a more potential condition to produce the target biotransformation product with a substrate conversion of 5.27%, which was selective for vanillyl methyl ketone at 93.17% and vanillin at 6.83%.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141673967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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