首页 > 最新文献

Engineering Headway最新文献

英文 中文
Expression and Characterization of Recombinant Endo-β-1,4-D-xylanases XynBTN63D from Soil Termite Abdomen in Escherichia coli BL21 (DE3) 土壤白蚁腹部重组内切-β-1,4-D-木聚糖酶 XynBTN63D 在大肠杆菌 BL21 (DE3) 中的表达与表征
Pub Date : 2024-07-05 DOI: 10.4028/p-5cqsmp
A. A. I. Ratnadewi, Sabella Muyasyaroh, Fatih Harum, Wuryanti Handayani, Sudarko Sudarko
The xynBTN63D gene sub-cloned on the plasmid shuttle vector pESC and pYHM1 in the host Escherichia coli BL21 (DE3) was successfully expressed and characterized. The xynBTN63D gene in the soluble fraction of each plasmid is expressed at induction temperatures of 25, 30, 35, 37, and 40 °C with a molecular weight of ±30 kDa. The soluble fraction of the xynBTN63D gene in both plasmids was expressed at induction temperatures of 25, 30, 35, 37, and 40°C with a molecular weight of ±30 kDa. The recombinant XynBTN63D, purified using the fast protein liquid chromatography (FPLC) method also has a molecular weight of ±30 kDa, observed using the sodium dodecyl polyacrylamide sodium electrophoresis (SDS-PAGE) method. The recombinant XynBTN63D has a temperature of 40 °C and an optimum pH of 5.5. It shows stability from 4 to 40 °C after preincubation for 1 hour with relative activity on the pCES and pMH1 plasmid of more than 50%. Recombinant XynBT63D also showed pH stability after being preincubated for 24 hours by showing relative activity of 86-100% at pH 5.0 to 6.0 for each plasmid
将 xynBTN63D 基因亚克隆到质粒穿梭载体 pESC 和 pYHM1 上,并在宿主大肠杆菌 BL21 (DE3) 中成功表达和鉴定。每个质粒可溶性部分中的 xynBTN63D 基因在 25、30、35、37 和 40 °C 的诱导温度下表达,分子量为 ±30 kDa。两个质粒中 xynBTN63D 基因的可溶性部分在 25、30、35、37 和 40 ℃ 的诱导温度下表达,分子量为 ±30 kDa。用快速蛋白质液相色谱法(FPLC)纯化的重组 XynBTN63D 的分子量也为±30 kDa,用十二烷基聚丙烯酰胺钠电泳法(SDS-PAGE)观察到的结果也是±30 kDa。重组 XynBTN63D 的温度为 40 °C,最适 pH 值为 5.5。在预孵育 1 小时后,它在 4 至 40 ℃ 范围内表现出稳定性,在 pCES 和 pMH1 质粒上的相对活性超过 50%。重组 XynBT63D 在预培养 24 小时后也显示出 pH 值稳定性,在 pH 值为 5.0 至 6.0 的条件下,每个质粒的相对活性为 86% 至 100%。
{"title":"Expression and Characterization of Recombinant Endo-β-1,4-D-xylanases XynBTN63D from Soil Termite Abdomen in Escherichia coli BL21 (DE3)","authors":"A. A. I. Ratnadewi, Sabella Muyasyaroh, Fatih Harum, Wuryanti Handayani, Sudarko Sudarko","doi":"10.4028/p-5cqsmp","DOIUrl":"https://doi.org/10.4028/p-5cqsmp","url":null,"abstract":"The xynBTN63D gene sub-cloned on the plasmid shuttle vector pESC and pYHM1 in the host Escherichia coli BL21 (DE3) was successfully expressed and characterized. The xynBTN63D gene in the soluble fraction of each plasmid is expressed at induction temperatures of 25, 30, 35, 37, and 40 °C with a molecular weight of ±30 kDa. The soluble fraction of the xynBTN63D gene in both plasmids was expressed at induction temperatures of 25, 30, 35, 37, and 40°C with a molecular weight of ±30 kDa. The recombinant XynBTN63D, purified using the fast protein liquid chromatography (FPLC) method also has a molecular weight of ±30 kDa, observed using the sodium dodecyl polyacrylamide sodium electrophoresis (SDS-PAGE) method. The recombinant XynBTN63D has a temperature of 40 °C and an optimum pH of 5.5. It shows stability from 4 to 40 °C after preincubation for 1 hour with relative activity on the pCES and pMH1 plasmid of more than 50%. Recombinant XynBT63D also showed pH stability after being preincubated for 24 hours by showing relative activity of 86-100% at pH 5.0 to 6.0 for each plasmid","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141674550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potential of the Consortium Bacteria as A Domestic Waste Water Bioremediation Agent 财团细菌作为生活污水生物修复剂的潜力
Pub Date : 2024-07-05 DOI: 10.4028/p-ftcg0e
Eli Hendrik Sanjaya, Annisa Elchamida, Dita Ayu Eka Saputri, S. Prabaningtyas, Agung Witjoro, Evi Susanti
Along with the increasing population in Indonesia, there has been an increase in domestic waste production. It is necessary to treat wastewater before it goes to the environment to prevent pollution of the environment. This research was conducted to investigate the potential of isolates A, B, and L from the Biology Department Universitas Negeri Malang as a bacterial consortium in decomposing domestic waste with the aerobic system to remove Chemical Oxygen Demand using a batch system. Experimental trials were carried out using synthetic domestic waste with variations in bacterial formulation treatments and starter doses. The results show that a more stable COD reduction performance was obtained in the batch test process. Sequentially, the COD reduction data efficiency includes B 20% (88.65%), L 20% (84.2%), A 10% (80%), A 20% (79.92%), ABL 10% (72.3%), B 10% (25.67%), ABL 20% (-10.87%), with the greatest efficiency occurring in the L10% treatment at 89.76%. The genotype analysis shows that A, B, and L isolates were identified as Alcaligenes ammonioxydans
随着印度尼西亚人口的不断增长,生活垃圾的产生量也在增加。有必要在废水进入环境之前对其进行处理,以防止对环境造成污染。本研究旨在调查来自马兰大学生物系的分离菌 A、B 和 L 作为细菌联合体在有氧系统中分解生活垃圾的潜力,以批处理系统去除化学需氧量。实验使用合成生活垃圾进行,细菌配方处理和启动剂剂量各不相同。结果表明,在批量试验过程中获得了更稳定的 COD 减排性能。COD 减排数据效率依次为 B 20%(88.65%)、L 20%(84.2%)、A 10%(80%)、A 20%(79.92%)、ABL 10%(72.3%)、B 10%(25.67%)、ABL 20%(-10.87%),其中 L 10%处理效率最高,为 89.76%。基因型分析表明,A、B 和 L 分离物被鉴定为氨氧化烯钙铝菌(Alcaligenes ammonioxydans
{"title":"Potential of the Consortium Bacteria as A Domestic Waste Water Bioremediation Agent","authors":"Eli Hendrik Sanjaya, Annisa Elchamida, Dita Ayu Eka Saputri, S. Prabaningtyas, Agung Witjoro, Evi Susanti","doi":"10.4028/p-ftcg0e","DOIUrl":"https://doi.org/10.4028/p-ftcg0e","url":null,"abstract":"Along with the increasing population in Indonesia, there has been an increase in domestic waste production. It is necessary to treat wastewater before it goes to the environment to prevent pollution of the environment. This research was conducted to investigate the potential of isolates A, B, and L from the Biology Department Universitas Negeri Malang as a bacterial consortium in decomposing domestic waste with the aerobic system to remove Chemical Oxygen Demand using a batch system. Experimental trials were carried out using synthetic domestic waste with variations in bacterial formulation treatments and starter doses. The results show that a more stable COD reduction performance was obtained in the batch test process. Sequentially, the COD reduction data efficiency includes B 20% (88.65%), L 20% (84.2%), A 10% (80%), A 20% (79.92%), ABL 10% (72.3%), B 10% (25.67%), ABL 20% (-10.87%), with the greatest efficiency occurring in the L10% treatment at 89.76%. The genotype analysis shows that A, B, and L isolates were identified as Alcaligenes ammonioxydans","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141673605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening Multitarget Anticancer Compounds from Salicylic Acid Derivatives: (Without and with Amino Acid Linkage) by In Silico Docking 通过硅对接从水杨酸衍生物中筛选多靶点抗癌化合物:(无氨基酸连接和有氨基酸连接
Pub Date : 2024-07-05 DOI: 10.4028/p-svkm5p
Warsito Warsito, M. Masruri, Sinta Murlistyarini, D. Pangesti, Asyfariatus Zulfa Azhar
This research aims to design anticancer molecules using the hybridization concept based on molecular derivatives of salicylic acid. The investigation explores structures with and without linked amino acid alanine through an in-silico docking approach. The research conducts screenings of the designed salicylic acid derivative molecules against receptors, including MMP9, MMP2, CDK2, P53, BAK EGFR, and ADP Ribose Polymerase. The most promising docking results for multitarget cancer compounds were observed in salicylic acid derivatives with amino acid linkages, specifically salicylic acid-curcumin, salicylic acid-benzyl alcohol, and salicylic acid-eugenol. These derivatives exhibited binding affinities towards MMP9 of -9.6, -9.6, and -8.9 kcal/mol, towards EGFR of -9.0, -7.6, and -7.9 kcal/mol, and ADP Ribose Polymerase of -11.2, -9.0, and -9.4 kcal/mol, respectively. The outcomes of the docking results highlight the significantly improved efficacy of multitarget anticancer compounds designed from salicylic acid derivatives with amino acid linkages, attributing this superiority to the enriched functional groups in the amino acid structure that enhance ligand-receptor interactions. This research contributes to identifying potential drug molecules as effective multitarget anticancer agents.
这项研究旨在利用基于水杨酸分子衍生物的杂交概念设计抗癌分子。该研究通过一种室内对接方法,探索了含有和不含连接氨基酸丙氨酸的结构。研究针对受体(包括 MMP9、MMP2、CDK2、P53、BAK 表皮生长因子受体和 ADP 核糖聚合酶)对所设计的水杨酸衍生物分子进行了筛选。具有氨基酸连接的水杨酸衍生物,特别是水杨酸-姜黄素、水杨酸-苄醇和水杨酸-丁香酚,是多靶点癌症化合物中最有希望的对接结果。这些衍生物与 MMP9 的结合亲和力分别为-9.6、-9.6 和-8.9 kcal/mol,与表皮生长因子受体的结合亲和力分别为-9.0、-7.6 和-7.9 kcal/mol,与 ADP 核糖聚合酶的结合亲和力分别为-11.2、-9.0 和-9.4 kcal/mol。对接结果表明,由带有氨基酸连接的水杨酸衍生物设计的多靶点抗癌化合物的疗效显著提高,其优越性归功于氨基酸结构中丰富的官能团增强了配体与受体之间的相互作用。这项研究有助于将潜在的药物分子鉴定为有效的多靶点抗癌剂。
{"title":"Screening Multitarget Anticancer Compounds from Salicylic Acid Derivatives: (Without and with Amino Acid Linkage) by In Silico Docking","authors":"Warsito Warsito, M. Masruri, Sinta Murlistyarini, D. Pangesti, Asyfariatus Zulfa Azhar","doi":"10.4028/p-svkm5p","DOIUrl":"https://doi.org/10.4028/p-svkm5p","url":null,"abstract":"This research aims to design anticancer molecules using the hybridization concept based on molecular derivatives of salicylic acid. The investigation explores structures with and without linked amino acid alanine through an in-silico docking approach. The research conducts screenings of the designed salicylic acid derivative molecules against receptors, including MMP9, MMP2, CDK2, P53, BAK EGFR, and ADP Ribose Polymerase. The most promising docking results for multitarget cancer compounds were observed in salicylic acid derivatives with amino acid linkages, specifically salicylic acid-curcumin, salicylic acid-benzyl alcohol, and salicylic acid-eugenol. These derivatives exhibited binding affinities towards MMP9 of -9.6, -9.6, and -8.9 kcal/mol, towards EGFR of -9.0, -7.6, and -7.9 kcal/mol, and ADP Ribose Polymerase of -11.2, -9.0, and -9.4 kcal/mol, respectively. The outcomes of the docking results highlight the significantly improved efficacy of multitarget anticancer compounds designed from salicylic acid derivatives with amino acid linkages, attributing this superiority to the enriched functional groups in the amino acid structure that enhance ligand-receptor interactions. This research contributes to identifying potential drug molecules as effective multitarget anticancer agents.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141673467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Binding Affinities of Hyptolide and its Derivatives onto HDAC Class IIa Protein from In Silico Analysis 从硅学分析看Hyptolide及其衍生物与HDAC IIa类蛋白质的结合亲和力
Pub Date : 2024-07-05 DOI: 10.4028/p-ii4amx
Lulut Tutik Margi Rahayu, Parsaoran Siahaan, M. Suzery
HDAC proteins play a role in epigenetic changes in cancer-causing gene expression. Previous research reported that hyptolide and its derivatives inhibited several cancer cell growths in vitro. However, the potential of this anticancer compounds in preventing HDAC protein activity has not yet been evaluated. This study aims to determine the site-related inhibitory activity and binding affinity produced by hyptolide against HDAC class IIa protein compared to its derivative complexes. Hyptolide and its derivatives have previously been optimized using ORCA software with B3LYP/6-31G** functional DFT theory. The optimization results on the hyptolide molecule show that the partial charge values of O4, O5, O6, and O7 atoms in the OAc group are -0.437; -0.444; -0.436, and -0.431 with a dipole moment 5.47363 Debye, while the partial charges of hyptolide derivatives on the same atom for epoxy-hyptolide are -0.465; -0.454; -0.448, and -0.415 with dipole moment value 8.57293 Debye and dimethylphenylamine-hyptolide molecules are -0.456; -0.442; -0.559 and -0.418 and dipole moment value 8.86020 Debye. The pharmacokinetics and class toxicity test of ligand compound show that the dimethylphenylamine-hyptolide has highest class toxicity, and it is more toxic than hyptolide and epoxy-hyptolide. The molecular docking process was conducted using AutoDock Vina software. The active site of amino acid residue that interacts with each ligand is Asp B 801 and Val B 708. In line with the partial charge value of the optimized hyptolide compound and its derivatives, binding affinity value complex with dimethylphenylamine-hyptolide ligand compound had the lowest binding affinitiy value in first conformation of -11.00. These results confirm that prediction of hyptolide derivative compounds with nitrogen group as alkylating agents will decrease the binding affinity between ligands and proteins. These results motivate researchers to conduct further studies on the activity of hyptolide derivative compounds in dimethylphenylamine-hyptolide against cancer cell growth in vitro and how to decrease its toxicity.
HDAC 蛋白在致癌基因表达的表观遗传变化中发挥作用。此前的研究报告称,百内酯及其衍生物在体外抑制了多种癌细胞的生长。然而,这种抗癌化合物在阻止 HDAC 蛋白活性方面的潜力尚未得到评估。本研究旨在确定Hyptolide与其衍生物复合物相比,对HDAC IIa类蛋白质产生的与位点相关的抑制活性和结合亲和力。之前已使用 ORCA 软件和 B3LYP/6-31G** 功能 DFT 理论对 Hyptolide 及其衍生物进行了优化。对 Hyptolide 分子的优化结果显示,OAc 基团中 O4、O5、O6 和 O7 原子的偏电荷值分别为 -0.437、-0.444、-0.436 和 -0.431,偶极矩为 5.47363 Debye,而环氧-庚内酯衍生物在同一原子上的偏电荷为-0.465;-0.454;-0.448 和-0.415,偶极矩值为 8.57293 Debye,二甲基苯胺-庚内酯分子的偏电荷为-0.456;-0.442;-0.559 和-0.418,偶极矩值为 8.86020 Debye。配体化合物的药代动力学和毒性测试表明,二甲基苯胺-庚内酯的毒性最高,且毒性高于庚内酯和环氧-庚内酯。分子对接过程采用 AutoDock Vina 软件进行。与各配体相互作用的氨基酸残基的活性位点分别为 Asp B 801 和 Val B 708。根据优化后的庚内酯化合物及其衍生物的部分电荷值,与二甲基苯胺-庚内酯配体化合物的结合亲和值在第一构象中最低,为-11.00。这些结果证实,预测含氮基团的庚内酯衍生物化合物作为烷化剂会降低配体与蛋白质之间的结合亲和力。这些结果促使研究人员进一步研究二甲基苯胺-庚内酯中的庚内酯衍生物化合物对体外癌细胞生长的活性以及如何降低其毒性。
{"title":"Binding Affinities of Hyptolide and its Derivatives onto HDAC Class IIa Protein from In Silico Analysis","authors":"Lulut Tutik Margi Rahayu, Parsaoran Siahaan, M. Suzery","doi":"10.4028/p-ii4amx","DOIUrl":"https://doi.org/10.4028/p-ii4amx","url":null,"abstract":"HDAC proteins play a role in epigenetic changes in cancer-causing gene expression. Previous research reported that hyptolide and its derivatives inhibited several cancer cell growths in vitro. However, the potential of this anticancer compounds in preventing HDAC protein activity has not yet been evaluated. This study aims to determine the site-related inhibitory activity and binding affinity produced by hyptolide against HDAC class IIa protein compared to its derivative complexes. Hyptolide and its derivatives have previously been optimized using ORCA software with B3LYP/6-31G** functional DFT theory. The optimization results on the hyptolide molecule show that the partial charge values of O4, O5, O6, and O7 atoms in the OAc group are -0.437; -0.444; -0.436, and -0.431 with a dipole moment 5.47363 Debye, while the partial charges of hyptolide derivatives on the same atom for epoxy-hyptolide are -0.465; -0.454; -0.448, and -0.415 with dipole moment value 8.57293 Debye and dimethylphenylamine-hyptolide molecules are -0.456; -0.442; -0.559 and -0.418 and dipole moment value 8.86020 Debye. The pharmacokinetics and class toxicity test of ligand compound show that the dimethylphenylamine-hyptolide has highest class toxicity, and it is more toxic than hyptolide and epoxy-hyptolide. The molecular docking process was conducted using AutoDock Vina software. The active site of amino acid residue that interacts with each ligand is Asp B 801 and Val B 708. In line with the partial charge value of the optimized hyptolide compound and its derivatives, binding affinity value complex with dimethylphenylamine-hyptolide ligand compound had the lowest binding affinitiy value in first conformation of -11.00. These results confirm that prediction of hyptolide derivative compounds with nitrogen group as alkylating agents will decrease the binding affinity between ligands and proteins. These results motivate researchers to conduct further studies on the activity of hyptolide derivative compounds in dimethylphenylamine-hyptolide against cancer cell growth in vitro and how to decrease its toxicity.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141674261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing Efficiency of Rural Cooking Stove: Numerical and Experimental Validation of Design and Development 提高农村炉灶的效率:设计与开发的数值和实验验证
Pub Date : 2024-06-14 DOI: 10.4028/p-idjms8
Firas Basim Ismail, Kareena Balan, Abdulkareem Abdulwahab, Hussein A. Kazem, M. Chaichan, Muhammad Aqil Afham Rahmat
The study presents the design of a highly efficient cooking stove for rural applications, along with performance evaluation. Drawing on diverse literature, existing works were analysed to derive design enhancements. Utilizing SolidWorks and Creo, a model was constructed, followed by material selection and cost analysis using accessible market components. ANSYS Fluent enabled flow analysis for both optimal and real configurations, complemented by experimental tests for validation. Results underscore the stove's efficiency, evident in reduced wood consumption for cooking and decreased soot generation. Significantly, the system's heat transfer rate reached 11.67°C/min, outperforming open stoves at 3.42°C/min, while maintaining affordability at RM 415.34. Nomenclature = Temperature transfer efficiency for heat recovery = Temperature outside air before entering system = Temperature inside air after system heat transfer has occurred = Temperature inside air before system is switched on = Temperature Transfer Efficiency for Heat Loss
本研究介绍了一种适用于农村的高效炉灶的设计和性能评估。该研究借鉴了各种文献,对现有作品进行了分析,以得出设计改进方案。利用 SolidWorks 和 Creo 构建了一个模型,然后利用市场上可买到的部件进行了材料选择和成本分析。ANSYS Fluent 可对最佳配置和实际配置进行流动分析,并辅以实验测试进行验证。结果表明,该炉灶的效率很高,烹饪时木材消耗量减少,烟尘产生量降低。值得注意的是,该系统的传热速率达到 11.67°C/分钟,超过了 3.42°C/min 的开放式炉灶,同时还保持了 415.34 令吉的经济性。术语 = 热量回收的温度传递效率 = 进入系统前的室外空气温度 = 系统热传递后的室内空气温度 = 系统开启前的室内空气温度 = 热量损失的温度传递效率
{"title":"Enhancing Efficiency of Rural Cooking Stove: Numerical and Experimental Validation of Design and Development","authors":"Firas Basim Ismail, Kareena Balan, Abdulkareem Abdulwahab, Hussein A. Kazem, M. Chaichan, Muhammad Aqil Afham Rahmat","doi":"10.4028/p-idjms8","DOIUrl":"https://doi.org/10.4028/p-idjms8","url":null,"abstract":"The study presents the design of a highly efficient cooking stove for rural applications, along with performance evaluation. Drawing on diverse literature, existing works were analysed to derive design enhancements. Utilizing SolidWorks and Creo, a model was constructed, followed by material selection and cost analysis using accessible market components. ANSYS Fluent enabled flow analysis for both optimal and real configurations, complemented by experimental tests for validation. Results underscore the stove's efficiency, evident in reduced wood consumption for cooking and decreased soot generation. Significantly, the system's heat transfer rate reached 11.67°C/min, outperforming open stoves at 3.42°C/min, while maintaining affordability at RM 415.34. Nomenclature = Temperature transfer efficiency for heat recovery = Temperature outside air before entering system = Temperature inside air after system heat transfer has occurred = Temperature inside air before system is switched on = Temperature Transfer Efficiency for Heat Loss","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141341316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Numerical Study of the Effect of Rotation on Backward-Facing Step 旋转对后向台阶影响的数值研究
Pub Date : 2024-06-14 DOI: 10.4028/p-nen027
Ghaidaa Kareem Rahi, Dhafer Hamza
A numerical simulation of the effect of rotation on mixed convection laminar flow with separation and reattachment length is studied. The channel is subjected to span-wise Rotation. The working fluid was air with the prandtl number of 0.71. The governing equations are solved with a finite element-based commercial solver, COMSOL Multiphysics. The influence of the Reynolds number and angular velocity Ω on the fluid flow and heat transfer characteristics is numerically studied. Range varies Reynolds number (100 ≤ Re ≤ 500), Richardson number (0.1 ≤ Ri ≤ 10), and angular velocity Ω (0, 10, 20, 30). The Nusselt number, pressure drop, recirculation length, and total flow rate were calculated. The calculated results span a wide parameters set, particularly from low rotational speed to high rotational speed. The Nusselt number, pressure drop, and patterns are shown. When comparing the results of the standard BFS case with the rotating BFS case in a step facing backward, the Heat Transfer Enhancement was 3% present obtained for rotation of Ω=30 at the Reynolds number Re=500.
研究了旋转对具有分离和重新附着长度的混合对流层流的影响的数值模拟。通道受到跨度旋转的影响。工作流体为空气,prandtl 数为 0.71。使用基于有限元的商用求解器 COMSOL Multiphysics 对控制方程进行求解。数值研究了雷诺数和角速度 Ω 对流体流动和传热特性的影响。雷诺数(100 ≤ Re ≤ 500)、理查德森数(0.1 ≤ Ri ≤ 10)和角速度 Ω 的变化范围为 0、10、20、30。计算了努塞尔特数、压降、再循环长度和总流量。计算结果的参数范围很广,特别是从低转速到高转速。图中显示了努塞尔特数、压降和模式。在雷诺数 Re=500 时,将标准 BFS 案例的结果与步进朝后的旋转 BFS 案例的结果进行比较,在 Ω=30 的旋转条件下,传热增强率为 3%。
{"title":"Numerical Study of the Effect of Rotation on Backward-Facing Step","authors":"Ghaidaa Kareem Rahi, Dhafer Hamza","doi":"10.4028/p-nen027","DOIUrl":"https://doi.org/10.4028/p-nen027","url":null,"abstract":"A numerical simulation of the effect of rotation on mixed convection laminar flow with separation and reattachment length is studied. The channel is subjected to span-wise Rotation. The working fluid was air with the prandtl number of 0.71. The governing equations are solved with a finite element-based commercial solver, COMSOL Multiphysics. The influence of the Reynolds number and angular velocity Ω on the fluid flow and heat transfer characteristics is numerically studied. Range varies Reynolds number (100 ≤ Re ≤ 500), Richardson number (0.1 ≤ Ri ≤ 10), and angular velocity Ω (0, 10, 20, 30). The Nusselt number, pressure drop, recirculation length, and total flow rate were calculated. The calculated results span a wide parameters set, particularly from low rotational speed to high rotational speed. The Nusselt number, pressure drop, and patterns are shown. When comparing the results of the standard BFS case with the rotating BFS case in a step facing backward, the Heat Transfer Enhancement was 3% present obtained for rotation of Ω=30 at the Reynolds number Re=500.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141342244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancement of Vapor Compression Refrigeration System by Adding Different Nano-Fluids: A Review 通过添加不同的纳米流体增强蒸汽压缩制冷系统:综述
Pub Date : 2024-06-14 DOI: 10.4028/p-0fnjhm
Dalya Shaker Qaid
Scientists from a variety of fields have joined forces to study a world so small that it cannot be seen with the naked eye, not even under a light microscope. That is the domain of nanotechnology, the universe of atoms and nanostructures. Anything between 1 and 100 nm in size is generally considered to be within the purview of nanotechnology, despite the occasional disagreement among experts regarding precisely what is included in the nanoscale. That is not the atomic scale and the microscale is still larger. This paper will define nanotechnology and discuss its potential future applications. The application of nanoparticles in one of the mechanical engineering field's most significant uses will also be reviewed. The Vapor Compression Refrigeration System (VCRS) uses nanobased refrigerants to deliver a better, faster cooling process with less power consumption. resulting in the provision of a healthier environment and a more effective system.
来自不同领域的科学家联手研究一个如此微小的世界,以至于无法用肉眼看到,甚至在光学显微镜下也无法看到。这就是纳米技术的领域,原子和纳米结构的宇宙。尺寸在 1 纳米到 100 纳米之间的任何东西一般都被认为属于纳米技术的范畴,尽管专家们有时会对纳米尺度所包括的具体内容产生分歧。这还不是原子尺度,而微米尺度则更大。本文将对纳米技术进行定义,并讨论其未来的潜在应用。本文还将回顾纳米粒子在机械工程领域最重要用途之一中的应用。蒸汽压缩制冷系统(VCRS)使用纳米制冷剂,以更低的能耗提供更好、更快的冷却过程,从而提供更健康的环境和更有效的系统。
{"title":"Enhancement of Vapor Compression Refrigeration System by Adding Different Nano-Fluids: A Review","authors":"Dalya Shaker Qaid","doi":"10.4028/p-0fnjhm","DOIUrl":"https://doi.org/10.4028/p-0fnjhm","url":null,"abstract":"Scientists from a variety of fields have joined forces to study a world so small that it cannot be seen with the naked eye, not even under a light microscope. That is the domain of nanotechnology, the universe of atoms and nanostructures. Anything between 1 and 100 nm in size is generally considered to be within the purview of nanotechnology, despite the occasional disagreement among experts regarding precisely what is included in the nanoscale. That is not the atomic scale and the microscale is still larger. This paper will define nanotechnology and discuss its potential future applications. The application of nanoparticles in one of the mechanical engineering field's most significant uses will also be reviewed. The Vapor Compression Refrigeration System (VCRS) uses nanobased refrigerants to deliver a better, faster cooling process with less power consumption. resulting in the provision of a healthier environment and a more effective system.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141343757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Numerical Analysis of Thermally Developing and Fully Developed Flow in Different Configurations of Microchannels 不同配置微通道中热发展和完全发展流动的数值分析
Pub Date : 2024-06-14 DOI: 10.4028/p-5s1akm
Ghufran Kahdem, Ahmed AL-Saadi
Microchannels based on Micro Electromechanical Systems (MEMS) have garnered a great lot of interest over the past 40 years from the fields of microfluidics and biomedicine. In order to address the problem of heat dissipation in incredibly small integrated circuits with up to 790 W/cm2 of huge heat dissipation capabilities, Tuckerman et al. [1] first proposed the microchannel heat rejection idea in 1981. These channels are typically at the microscale, with characteristic dimensions on the order of micrometers 10-6m or smaller as shown in Figure 1 [2]. These channels often exist at the microscale and have characteristic diameters of micrometres 10-6m or less. Microchannels display distinct fluidic behaviour as a result of their small size. Because the flow is normally laminar and the Reynolds number is low, the flow pattern is predictable. Capillary forces and other surface forces become comparatively more important. As a result, fluid behaviour in microchannels is greatly influenced by surface wetting characteristics and channel surface roughness.
过去 40 年来,基于微机电系统(MEMS)的微通道在微流控和生物医学领域引起了广泛关注。为了解决散热能力高达 790 W/cm2 的超小型集成电路的散热问题,Tuckerman 等人[1] 于 1981 年首次提出了微通道散热理念。如图 1[2]所示,这些通道通常为微米级,其特征尺寸为微米 10-6m 或更小。这些通道通常处于微尺度,其特征直径为 10-6m 微米或更小。微通道因其尺寸小而显示出独特的流体特性。由于流动通常是层流且雷诺数较低,因此流动模式是可预测的。相对而言,毛细力和其他表面力变得更加重要。因此,微通道中的流体特性在很大程度上受到表面润湿特性和通道表面粗糙度的影响。
{"title":"Numerical Analysis of Thermally Developing and Fully Developed Flow in Different Configurations of Microchannels","authors":"Ghufran Kahdem, Ahmed AL-Saadi","doi":"10.4028/p-5s1akm","DOIUrl":"https://doi.org/10.4028/p-5s1akm","url":null,"abstract":"Microchannels based on Micro Electromechanical Systems (MEMS) have garnered a great lot of interest over the past 40 years from the fields of microfluidics and biomedicine. In order to address the problem of heat dissipation in incredibly small integrated circuits with up to 790 W/cm2 of huge heat dissipation capabilities, Tuckerman et al. [1] first proposed the microchannel heat rejection idea in 1981. These channels are typically at the microscale, with characteristic dimensions on the order of micrometers 10-6m or smaller as shown in Figure 1 [2]. These channels often exist at the microscale and have characteristic diameters of micrometres 10-6m or less. Microchannels display distinct fluidic behaviour as a result of their small size. Because the flow is normally laminar and the Reynolds number is low, the flow pattern is predictable. Capillary forces and other surface forces become comparatively more important. As a result, fluid behaviour in microchannels is greatly influenced by surface wetting characteristics and channel surface roughness.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141339044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Thermal Stratification in Solar Water Storage Tanks through Inlet Port Diffuser Optimization during Charge and Discharge Processes 在充水和排水过程中通过优化进水口扩散器实现太阳能储水箱的热分层
Pub Date : 2024-06-14 DOI: 10.4028/p-1r0mvz
Elaf Sadeq Barrak, Karrar A. Hammodi, Ahmed Mouthanna
The enhancement of thermal stratification in solar storage tanks is a crucial aspect of advancing solar energy technology. This study presents an experimental investigation conducted on a vertical cylindrical hot solar water storage tank operating in a dynamic mode, involving simultaneous charging and discharging. The experiment aims to assess the impact of varying inlet water flow rates (4, 6, 8, 10 L/min) on thermal stratification within the tank and explores strategies to mitigate heat losses to the environment and minimize mixing effects caused by the inlet fluid flow. The experimental setup incorporates insulation and an inlet port diffuser section to optimize the distribution of inlet water during the charging and discharging states. The results reveal that the utilization of an inlet port diffuser significantly improves thermal stratification by reducing heat losses and minimizing mixing effects, as evidenced by improved Richardson numbers. Additionally, this study contributes to the development of a compact solar domestic hot water (SDHW) solution.
增强太阳能储水箱中的热分层是太阳能技术发展的一个重要方面。本研究介绍了对一个以动态模式运行的立式圆柱形太阳能热储水罐进行的实验研究,包括同时充水和放水。实验旨在评估不同进水流速(4、6、8、10 升/分钟)对储热水箱内热分层的影响,并探索减少热量损失到环境中的策略,以及最大限度地减少进水流体流动造成的混合效应。实验装置包括隔热层和进水口扩散器部分,以优化充水和排水状态下的进水分布。结果表明,利用进水口扩散器可减少热量损失并将混合效应降至最低,从而显著改善热分层,理查德森数值的提高就是证明。此外,这项研究还有助于开发一种紧凑型太阳能家用热水(SDHW)解决方案。
{"title":"Thermal Stratification in Solar Water Storage Tanks through Inlet Port Diffuser Optimization during Charge and Discharge Processes","authors":"Elaf Sadeq Barrak, Karrar A. Hammodi, Ahmed Mouthanna","doi":"10.4028/p-1r0mvz","DOIUrl":"https://doi.org/10.4028/p-1r0mvz","url":null,"abstract":"The enhancement of thermal stratification in solar storage tanks is a crucial aspect of advancing solar energy technology. This study presents an experimental investigation conducted on a vertical cylindrical hot solar water storage tank operating in a dynamic mode, involving simultaneous charging and discharging. The experiment aims to assess the impact of varying inlet water flow rates (4, 6, 8, 10 L/min) on thermal stratification within the tank and explores strategies to mitigate heat losses to the environment and minimize mixing effects caused by the inlet fluid flow. The experimental setup incorporates insulation and an inlet port diffuser section to optimize the distribution of inlet water during the charging and discharging states. The results reveal that the utilization of an inlet port diffuser significantly improves thermal stratification by reducing heat losses and minimizing mixing effects, as evidenced by improved Richardson numbers. Additionally, this study contributes to the development of a compact solar domestic hot water (SDHW) solution.","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141344251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A New Main Matrix to Find the Principal Stresses, Strains and the Max - Shear Stress, Strain by MATLAB Simulation 通过 MATLAB 仿真计算主应力、应变和最大剪应力、应变的新主矩阵
Pub Date : 2024-06-14 DOI: 10.4028/p-clvs2s
Abdullah Dhayea Assi
In this research, a main matrix has been derived from the stress components in the inclined plane with an angle θ that calculates the normal stresses and shear stress in the inclined plane to be used in calculating the main stresses and the maximum shear stress in one step, this is done using the principal stress angle which is calculated from known stress compounds. As well as calculating the maximum shear stress after using the correction factor. The inverse of this derived matrix can be used to calculate the stress compounds through the known principal stresses and this applies to the components of the principal strain and strain, and the maximum shear strain. All this is done through MATLAB simulation Keywords: Normal Stresses and Strains, Shear, Principal Stresses, max. Shear Stress
在这项研究中,根据倾斜面中的应力分量得出了一个主矩阵,该矩阵可以计算出倾斜面中的法向应力和剪应力,用于一步计算主应力和最大剪应力。在使用修正系数后,还可计算出最大剪应力。该推导矩阵的逆矩阵可用于通过已知主应力计算应力化合物,并适用于主应变和应变分量以及最大剪切应变。所有这些都通过 MATLAB 仿真完成:法向应力和应变,剪切,主应力,最大剪切应力剪应力
{"title":"A New Main Matrix to Find the Principal Stresses, Strains and the Max - Shear Stress, Strain by MATLAB Simulation","authors":"Abdullah Dhayea Assi","doi":"10.4028/p-clvs2s","DOIUrl":"https://doi.org/10.4028/p-clvs2s","url":null,"abstract":"In this research, a main matrix has been derived from the stress components in the inclined plane with an angle θ that calculates the normal stresses and shear stress in the inclined plane to be used in calculating the main stresses and the maximum shear stress in one step, this is done using the principal stress angle which is calculated from known stress compounds. As well as calculating the maximum shear stress after using the correction factor. The inverse of this derived matrix can be used to calculate the stress compounds through the known principal stresses and this applies to the components of the principal strain and strain, and the maximum shear strain. All this is done through MATLAB simulation Keywords: Normal Stresses and Strains, Shear, Principal Stresses, max. Shear Stress","PeriodicalId":512976,"journal":{"name":"Engineering Headway","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141339891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Engineering Headway
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1