Pub Date : 2015-01-01Epub Date: 2015-07-29DOI: 10.1155/2015/284986
Daniela Gandolfi, Jonathan Mapelli, Egidio D'Angelo
Understanding the spatiotemporal organization of long-term synaptic plasticity in neuronal networks demands techniques capable of monitoring changes in synaptic responsiveness over extended multineuronal structures. Among these techniques, voltage-sensitive dye imaging (VSD imaging) is of particular interest due to its good spatial resolution. However, improvements of the technique are needed in order to overcome limits imposed by its low signal-to-noise ratio. Here, we show that VSD imaging can detect long-term potentiation (LTP) and long-term depression (LTD) in acute cerebellar slices. Combined VSD imaging and patch-clamp recordings revealed that the most excited regions were predominantly associated with granule cells (GrCs) generating EPSP-spike complexes, while poorly responding regions were associated with GrCs generating EPSPs only. The correspondence with cellular changes occurring during LTP and LTD was highlighted by a vector representation obtained by combining amplitude with time-to-peak of VSD signals. This showed that LTP occurred in the most excited regions lying in the core of activated areas and increased the number of EPSP-spike complexes, while LTD occurred in the less excited regions lying in the surround. VSD imaging appears to be an efficient tool for investigating how synaptic plasticity contributes to the reorganization of multineuronal activity in neuronal circuits.
{"title":"Long-Term Spatiotemporal Reconfiguration of Neuronal Activity Revealed by Voltage-Sensitive Dye Imaging in the Cerebellar Granular Layer.","authors":"Daniela Gandolfi, Jonathan Mapelli, Egidio D'Angelo","doi":"10.1155/2015/284986","DOIUrl":"https://doi.org/10.1155/2015/284986","url":null,"abstract":"<p><p>Understanding the spatiotemporal organization of long-term synaptic plasticity in neuronal networks demands techniques capable of monitoring changes in synaptic responsiveness over extended multineuronal structures. Among these techniques, voltage-sensitive dye imaging (VSD imaging) is of particular interest due to its good spatial resolution. However, improvements of the technique are needed in order to overcome limits imposed by its low signal-to-noise ratio. Here, we show that VSD imaging can detect long-term potentiation (LTP) and long-term depression (LTD) in acute cerebellar slices. Combined VSD imaging and patch-clamp recordings revealed that the most excited regions were predominantly associated with granule cells (GrCs) generating EPSP-spike complexes, while poorly responding regions were associated with GrCs generating EPSPs only. The correspondence with cellular changes occurring during LTP and LTD was highlighted by a vector representation obtained by combining amplitude with time-to-peak of VSD signals. This showed that LTP occurred in the most excited regions lying in the core of activated areas and increased the number of EPSP-spike complexes, while LTD occurred in the less excited regions lying in the surround. VSD imaging appears to be an efficient tool for investigating how synaptic plasticity contributes to the reorganization of multineuronal activity in neuronal circuits. </p>","PeriodicalId":51299,"journal":{"name":"Neural Plasticity","volume":"2015 ","pages":"284986"},"PeriodicalIF":3.1,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/284986","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34007313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01Epub Date: 2015-08-26DOI: 10.1155/2015/846589
Darya V Bazovkina, Elena M Kondaurova, Vladimir S Naumenko, Evgeni Ponimaskin
In the present study behavioral effects of the 5-HT2C serotonin receptor were investigated in different mouse strains. The 5-HT2C receptor agonist MK-212 applied intraperitoneally induced significant dose-dependent reduction of distance traveled in the open field test in CBA/Lac mice. This effect was receptor-specific because it was inhibited by the 5-HT2C receptor antagonist RS102221. To study the role of genotype in 5-HT2C receptor-induced hypolocomotion, locomotor activity of seven inbred mouse strains was measured after MK-212 acute treatment. We found that the 5-HT2C receptor stimulation by MK-212 decreased distance traveled in the open field test in CBA/Lac, C57Bl/6, C3H/He, and ICR mice, whereas it failed to affect locomotor activity in DBA/2J, Asn, and Balb/c mice. We also compared the interstrain differences in functional response to 5-HT2C and 5-HT2A receptors activation measured by the quantification of receptor-mediated head-twitches. These experiments revealed significant positive correlation between 5-HT2C and 5-HT2A receptors functional responses for all investigated mouse strains. Moreover, we found that 5-HT2A receptor activation with DOI did not change locomotor activity in CBA/Lac mice. Taken together, our data indicate the implication of 5-HT2C receptors in regulation of locomotor activity and suggest the shared mechanism for functional responses mediated by 5-HT2C and 5-HT2A receptors.
{"title":"Genotype-Dependent Difference in 5-HT2C Receptor-Induced Hypolocomotion: Comparison with 5-HT2A Receptor Functional Activity.","authors":"Darya V Bazovkina, Elena M Kondaurova, Vladimir S Naumenko, Evgeni Ponimaskin","doi":"10.1155/2015/846589","DOIUrl":"https://doi.org/10.1155/2015/846589","url":null,"abstract":"<p><p>In the present study behavioral effects of the 5-HT2C serotonin receptor were investigated in different mouse strains. The 5-HT2C receptor agonist MK-212 applied intraperitoneally induced significant dose-dependent reduction of distance traveled in the open field test in CBA/Lac mice. This effect was receptor-specific because it was inhibited by the 5-HT2C receptor antagonist RS102221. To study the role of genotype in 5-HT2C receptor-induced hypolocomotion, locomotor activity of seven inbred mouse strains was measured after MK-212 acute treatment. We found that the 5-HT2C receptor stimulation by MK-212 decreased distance traveled in the open field test in CBA/Lac, C57Bl/6, C3H/He, and ICR mice, whereas it failed to affect locomotor activity in DBA/2J, Asn, and Balb/c mice. We also compared the interstrain differences in functional response to 5-HT2C and 5-HT2A receptors activation measured by the quantification of receptor-mediated head-twitches. These experiments revealed significant positive correlation between 5-HT2C and 5-HT2A receptors functional responses for all investigated mouse strains. Moreover, we found that 5-HT2A receptor activation with DOI did not change locomotor activity in CBA/Lac mice. Taken together, our data indicate the implication of 5-HT2C receptors in regulation of locomotor activity and suggest the shared mechanism for functional responses mediated by 5-HT2C and 5-HT2A receptors. </p>","PeriodicalId":51299,"journal":{"name":"Neural Plasticity","volume":"2015 ","pages":"846589"},"PeriodicalIF":3.1,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/846589","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34013211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01Epub Date: 2015-08-20DOI: 10.1155/2015/523250
Tobias Vogt, Rainer Herpers, Christopher D Askew, David Scherfgen, Heiko K Strüder, Stefan Schneider
Virtual reality environments are increasingly being used to encourage individuals to exercise more regularly, including as part of treatment those with mental health or neurological disorders. The success of virtual environments likely depends on whether a sense of presence can be established, where participants become fully immersed in the virtual environment. Exposure to virtual environments is associated with physiological responses, including cortical activation changes. Whether the addition of a real exercise within a virtual environment alters sense of presence perception, or the accompanying physiological changes, is not known. In a randomized and controlled study design, moderate-intensity Exercise (i.e., self-paced cycling) and No-Exercise (i.e., automatic propulsion) trials were performed within three levels of virtual environment exposure. Each trial was 5 minutes in duration and was followed by posttrial assessments of heart rate, perceived sense of presence, EEG, and mental state. Changes in psychological strain and physical state were generally mirrored by neural activation patterns. Furthermore, these changes indicated that exercise augments the demands of virtual environment exposures and this likely contributed to an enhanced sense of presence.
{"title":"Effects of Exercise in Immersive Virtual Environments on Cortical Neural Oscillations and Mental State.","authors":"Tobias Vogt, Rainer Herpers, Christopher D Askew, David Scherfgen, Heiko K Strüder, Stefan Schneider","doi":"10.1155/2015/523250","DOIUrl":"10.1155/2015/523250","url":null,"abstract":"<p><p>Virtual reality environments are increasingly being used to encourage individuals to exercise more regularly, including as part of treatment those with mental health or neurological disorders. The success of virtual environments likely depends on whether a sense of presence can be established, where participants become fully immersed in the virtual environment. Exposure to virtual environments is associated with physiological responses, including cortical activation changes. Whether the addition of a real exercise within a virtual environment alters sense of presence perception, or the accompanying physiological changes, is not known. In a randomized and controlled study design, moderate-intensity Exercise (i.e., self-paced cycling) and No-Exercise (i.e., automatic propulsion) trials were performed within three levels of virtual environment exposure. Each trial was 5 minutes in duration and was followed by posttrial assessments of heart rate, perceived sense of presence, EEG, and mental state. Changes in psychological strain and physical state were generally mirrored by neural activation patterns. Furthermore, these changes indicated that exercise augments the demands of virtual environment exposures and this likely contributed to an enhanced sense of presence. </p>","PeriodicalId":51299,"journal":{"name":"Neural Plasticity","volume":"2015 ","pages":"523250"},"PeriodicalIF":3.1,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34068311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01Epub Date: 2015-05-14DOI: 10.1155/2015/692541
Greicy Coelho de Souza, Julia Ariana de S Gomes, Ana Isabelle de Góis Queiroz, Maíra Morais de Araújo, Lígia Menezes Cavalcante, Michel de Jesus Souza Machado, Aline Santos Monte, David Freitas de Lucena, João Quevedo, André Ferrer Carvalho, Danielle Macêdo
Oxidative imbalance, alterations in brain-derived neurotrophic factor (BDNF), and mitochondrial dysfunction are implicated in bipolar disorder (BD) pathophysiology and comorbidities, for example, cardiovascular conditions. Carvedilol (CVD), a nonselective beta-blocker widely used for the treatment of hypertension, presents antioxidant and mitochondrial stabilizing properties. Thus, we hypothesized that CVD would prevent and/or reverse mania-like behavioral and neurochemical alterations induced by lisdexamfetamine dimesylate (LDX). To do this, male Wistar rats were submitted to two different protocols, namely, prevention and reversal. In the prevention treatment the rats received daily oral administration (mg/kg) of CVD (2.5, 5 or 7.5), saline, valproate (VAL200), or the combination of CVD5 + VAL100 for 7 days. From the 8th to 14th day LDX was added. In the reversal protocol LDX was administered for 7 days with the drugs being added from the 8th to 14th day of treatment. Two hours after the last administration the behavioral (open field and social interaction) and neurochemical (reduced glutathione, lipid peroxidation, and BDNF) determinations were performed. The results showed that CVD prevented and reversed the behavioral and neurochemical alterations induced by LDX. The administration of CVD5 + VAL100 potentiated the effect of VAL200 alone. Taken together these results demonstrate a possible antimanic effect of CVD in this preclinical model.
{"title":"Preclinical Evidences for an Antimanic Effect of Carvedilol.","authors":"Greicy Coelho de Souza, Julia Ariana de S Gomes, Ana Isabelle de Góis Queiroz, Maíra Morais de Araújo, Lígia Menezes Cavalcante, Michel de Jesus Souza Machado, Aline Santos Monte, David Freitas de Lucena, João Quevedo, André Ferrer Carvalho, Danielle Macêdo","doi":"10.1155/2015/692541","DOIUrl":"10.1155/2015/692541","url":null,"abstract":"<p><p>Oxidative imbalance, alterations in brain-derived neurotrophic factor (BDNF), and mitochondrial dysfunction are implicated in bipolar disorder (BD) pathophysiology and comorbidities, for example, cardiovascular conditions. Carvedilol (CVD), a nonselective beta-blocker widely used for the treatment of hypertension, presents antioxidant and mitochondrial stabilizing properties. Thus, we hypothesized that CVD would prevent and/or reverse mania-like behavioral and neurochemical alterations induced by lisdexamfetamine dimesylate (LDX). To do this, male Wistar rats were submitted to two different protocols, namely, prevention and reversal. In the prevention treatment the rats received daily oral administration (mg/kg) of CVD (2.5, 5 or 7.5), saline, valproate (VAL200), or the combination of CVD5 + VAL100 for 7 days. From the 8th to 14th day LDX was added. In the reversal protocol LDX was administered for 7 days with the drugs being added from the 8th to 14th day of treatment. Two hours after the last administration the behavioral (open field and social interaction) and neurochemical (reduced glutathione, lipid peroxidation, and BDNF) determinations were performed. The results showed that CVD prevented and reversed the behavioral and neurochemical alterations induced by LDX. The administration of CVD5 + VAL100 potentiated the effect of VAL200 alone. Taken together these results demonstrate a possible antimanic effect of CVD in this preclinical model. </p>","PeriodicalId":51299,"journal":{"name":"Neural Plasticity","volume":"2015 ","pages":"692541"},"PeriodicalIF":3.1,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4446493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34205686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01Epub Date: 2015-08-31DOI: 10.1155/2015/462182
Ying-Zu Huang, Yao-Shun Chang, Miao-Ju Hsu, Alice M K Wong, Ya-Ju Chang
Disrupted triphasic electromyography (EMG) patterns of agonist and antagonist muscle pairs during fast goal-directed movements have been found in patients with hypermetria. Since peripheral electrical stimulation (ES) and motor training may modulate motor cortical excitability through plasticity mechanisms, we aimed to investigate whether temporal ES-assisted movement training could influence premovement cortical excitability and alleviate hypermetria in patients with spinal cerebellar ataxia (SCA). The EMG of the agonist extensor carpi radialis muscle and antagonist flexor carpi radialis muscle, premovement motor evoked potentials (MEPs) of the flexor carpi radialis muscle, and the constant and variable errors of movements were assessed before and after 4 weeks of ES-assisted fast goal-directed wrist extension training in the training group and of general health education in the control group. After training, the premovement MEPs of the antagonist muscle were facilitated at 50 ms before the onset of movement. In addition, the EMG onset latency of the antagonist muscle shifted earlier and the constant error decreased significantly. In summary, temporal ES-assisted training alleviated hypermetria by restoring antagonist premovement and temporal triphasic EMG patterns in SCA patients. This technique may be applied to treat hypermetria in cerebellar disorders. (This trial is registered with NCT01983670.).
{"title":"Restoration of Central Programmed Movement Pattern by Temporal Electrical Stimulation-Assisted Training in Patients with Spinal Cerebellar Atrophy.","authors":"Ying-Zu Huang, Yao-Shun Chang, Miao-Ju Hsu, Alice M K Wong, Ya-Ju Chang","doi":"10.1155/2015/462182","DOIUrl":"https://doi.org/10.1155/2015/462182","url":null,"abstract":"<p><p>Disrupted triphasic electromyography (EMG) patterns of agonist and antagonist muscle pairs during fast goal-directed movements have been found in patients with hypermetria. Since peripheral electrical stimulation (ES) and motor training may modulate motor cortical excitability through plasticity mechanisms, we aimed to investigate whether temporal ES-assisted movement training could influence premovement cortical excitability and alleviate hypermetria in patients with spinal cerebellar ataxia (SCA). The EMG of the agonist extensor carpi radialis muscle and antagonist flexor carpi radialis muscle, premovement motor evoked potentials (MEPs) of the flexor carpi radialis muscle, and the constant and variable errors of movements were assessed before and after 4 weeks of ES-assisted fast goal-directed wrist extension training in the training group and of general health education in the control group. After training, the premovement MEPs of the antagonist muscle were facilitated at 50 ms before the onset of movement. In addition, the EMG onset latency of the antagonist muscle shifted earlier and the constant error decreased significantly. In summary, temporal ES-assisted training alleviated hypermetria by restoring antagonist premovement and temporal triphasic EMG patterns in SCA patients. This technique may be applied to treat hypermetria in cerebellar disorders. (This trial is registered with NCT01983670.).</p>","PeriodicalId":51299,"journal":{"name":"Neural Plasticity","volume":"2015 ","pages":"462182"},"PeriodicalIF":3.1,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/462182","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34045480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01Epub Date: 2015-08-31DOI: 10.1155/2015/754864
Marta Michalczuk, Beata Urban, Beata Chrzanowska-Grenda, Monika Oziębło-Kupczyk, Alina Bakunowicz-Łazarczyk
Purpose: The objective of our study was to examine a possible influence of gestational age, birth weight, and Apgar score on amplitudes and latencies of P100 wave in preterm born school-age children.
Materials and methods: We examined the following group of school-age children: 28 with history of prematurity (mean age 10.56 ± 1.66 years) and 25 born at term (mean age 11.2 ± 1.94 years). The monocular PVEP was performed in all children.
Results: The P100 wave amplitudes and latencies significantly differ between preterm born school-age children and those born at term. There was an essential positive linear correlation of the P100 wave amplitudes with birth weight, gestational age, and Apgar score. There were the negative linear correlations of P100 latencies in 15-minute stimulation from O1 and Oz electrode with Apgar score and O1 and O2 electrode with gestational age.
Conclusions: PVEP responses vary in preterm born children in comparison to term. Low birth weight, early gestational age, and poor baseline output seem to be the predicting factors for the developmental rate of a brain function in children with history of prematurity. Further investigations are necessary to determine perinatal factors that can affect the modified visual system function in preterm born children.
{"title":"An Influence of Birth Weight, Gestational Age, and Apgar Score on Pattern Visual Evoked Potentials in Children with History of Prematurity.","authors":"Marta Michalczuk, Beata Urban, Beata Chrzanowska-Grenda, Monika Oziębło-Kupczyk, Alina Bakunowicz-Łazarczyk","doi":"10.1155/2015/754864","DOIUrl":"https://doi.org/10.1155/2015/754864","url":null,"abstract":"<p><strong>Purpose: </strong>The objective of our study was to examine a possible influence of gestational age, birth weight, and Apgar score on amplitudes and latencies of P100 wave in preterm born school-age children.</p><p><strong>Materials and methods: </strong>We examined the following group of school-age children: 28 with history of prematurity (mean age 10.56 ± 1.66 years) and 25 born at term (mean age 11.2 ± 1.94 years). The monocular PVEP was performed in all children.</p><p><strong>Results: </strong>The P100 wave amplitudes and latencies significantly differ between preterm born school-age children and those born at term. There was an essential positive linear correlation of the P100 wave amplitudes with birth weight, gestational age, and Apgar score. There were the negative linear correlations of P100 latencies in 15-minute stimulation from O1 and Oz electrode with Apgar score and O1 and O2 electrode with gestational age.</p><p><strong>Conclusions: </strong>PVEP responses vary in preterm born children in comparison to term. Low birth weight, early gestational age, and poor baseline output seem to be the predicting factors for the developmental rate of a brain function in children with history of prematurity. Further investigations are necessary to determine perinatal factors that can affect the modified visual system function in preterm born children.</p>","PeriodicalId":51299,"journal":{"name":"Neural Plasticity","volume":"2015 ","pages":"754864"},"PeriodicalIF":3.1,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/754864","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34045482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01Epub Date: 2015-08-04DOI: 10.1155/2015/732014
Robert E Sims, John B Butcher, H Rheinallt Parri, Stanislaw Glazewski
Changing the whisker complement on a rodent's snout can lead to two forms of experience-dependent plasticity (EDP) in the neurons of the barrel cortex, where whiskers are somatotopically represented. One form, termed coding plasticity, concerns changes in synaptic transmission and connectivity between neurons. This is thought to underlie learning and memory processes and so adaptation to a changing environment. The second, called homeostatic plasticity, serves to maintain a restricted dynamic range of neuronal activity thus preventing its saturation or total downregulation. Current explanatory models of cortical EDP are almost exclusively neurocentric. However, in recent years, increasing evidence has emerged on the role of astrocytes in brain function, including plasticity. Indeed, astrocytes appear as necessary partners of neurons at the core of the mechanisms of coding and homeostatic plasticity recorded in neurons. In addition to neuronal plasticity, several different forms of astrocytic plasticity have recently been discovered. They extend from changes in receptor expression and dynamic changes in morphology to alteration in gliotransmitter release. It is however unclear how astrocytic plasticity contributes to the neuronal EDP. Here, we review the known and possible roles for astrocytes in the barrel cortex, including its plasticity.
{"title":"Astrocyte and Neuronal Plasticity in the Somatosensory System.","authors":"Robert E Sims, John B Butcher, H Rheinallt Parri, Stanislaw Glazewski","doi":"10.1155/2015/732014","DOIUrl":"https://doi.org/10.1155/2015/732014","url":null,"abstract":"<p><p>Changing the whisker complement on a rodent's snout can lead to two forms of experience-dependent plasticity (EDP) in the neurons of the barrel cortex, where whiskers are somatotopically represented. One form, termed coding plasticity, concerns changes in synaptic transmission and connectivity between neurons. This is thought to underlie learning and memory processes and so adaptation to a changing environment. The second, called homeostatic plasticity, serves to maintain a restricted dynamic range of neuronal activity thus preventing its saturation or total downregulation. Current explanatory models of cortical EDP are almost exclusively neurocentric. However, in recent years, increasing evidence has emerged on the role of astrocytes in brain function, including plasticity. Indeed, astrocytes appear as necessary partners of neurons at the core of the mechanisms of coding and homeostatic plasticity recorded in neurons. In addition to neuronal plasticity, several different forms of astrocytic plasticity have recently been discovered. They extend from changes in receptor expression and dynamic changes in morphology to alteration in gliotransmitter release. It is however unclear how astrocytic plasticity contributes to the neuronal EDP. Here, we review the known and possible roles for astrocytes in the barrel cortex, including its plasticity. </p>","PeriodicalId":51299,"journal":{"name":"Neural Plasticity","volume":"2015 ","pages":"732014"},"PeriodicalIF":3.1,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/732014","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33983445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01Epub Date: 2015-08-05DOI: 10.1155/2015/723891
Tomas C Bellamy, Anna Dunaevsky, H Rheinallt Parri
Over the last few decades, our understanding of the roles of glial cells in the central nervous system has been transformed. There is now a clear consensus that all classes of glia (astrocytes, oligodendrocytes, microglia, and various other progenitors and specialized cells) can detect and respond to a wide range of neurotransmitters, hormones, cytokines, and trophic factors and thereby play an active, signaling role in neurophysiology. To date, much of the focus of glial signaling has been on how glia can influence the function of the neuronal network with the associated impact on information processing and, ultimately, behavior. In particular, the contribution of bidirectional communication between neurons and glia to the regulation of synaptic plasticity has been extensively studied. The papers collected in this special issue focus on a related, but distinct, question: can glia themselves exhibit activity-dependent plasticity? The reviews and experimental papers present the evidence that glia do indeed have the capacity to respond dynamically to a wide range of stimuli with persistent changes in signal transduction, morphology, and homeostasis. In " Plasticity of Neuron-Glial Transmission: Equipping Glia for Long-Term Integration of Network Activity, " W. Croft et al. review the current evidence for plasticity in neuron-glial communication and speculate on the implication of differences in induction paradigms from synaptic plasticity for the computational properties of glial signaling. In " Glutamatergic Transmission: A Matter of Three, " Z. Martínez-Lozada and A. Ortega focus on the consequences of glutamate receptor activation for astroglial physiology. By identifying the downstream targets engaged by gluta-matergic signalling, the authors show how neurons can shape transcriptional and translational regulation in glia to tailor transmitter clearance and recycling to meet synaptic demands. Remaining with regulation of intracellular signaling in astrocytes, N. Komin et al. present an analysis of the impact of variation in uptake of calcium into endoplasmic reticulum stores on cytoplasmic calcium oscillations in " Multiscale Modeling Indicates That Temperature Dependent [Ca 2+ ] i Spiking in Astrocytes Is Quantitatively Consistent with Modulated SERCA Activity. " The results of the modelling study illustrate the striking sensitivity of intracellular calcium dynamics to changes in SERCA activity with implications both for interpretation of experimental results at nonphys-iological temperatures and for prediction of calcium signal kinetics in vivo. In " Fractalkine Signaling and Microglia Functions in the Developing Brain, " I. Arnoux and E. Audinat review the effects of fractalkine receptor activation on microglial function. The review …
{"title":"Glial Plasticity.","authors":"Tomas C Bellamy, Anna Dunaevsky, H Rheinallt Parri","doi":"10.1155/2015/723891","DOIUrl":"https://doi.org/10.1155/2015/723891","url":null,"abstract":"Over the last few decades, our understanding of the roles of glial cells in the central nervous system has been transformed. There is now a clear consensus that all classes of glia (astrocytes, oligodendrocytes, microglia, and various other progenitors and specialized cells) can detect and respond to a wide range of neurotransmitters, hormones, cytokines, and trophic factors and thereby play an active, signaling role in neurophysiology. To date, much of the focus of glial signaling has been on how glia can influence the function of the neuronal network with the associated impact on information processing and, ultimately, behavior. In particular, the contribution of bidirectional communication between neurons and glia to the regulation of synaptic plasticity has been extensively studied. The papers collected in this special issue focus on a related, but distinct, question: can glia themselves exhibit activity-dependent plasticity? The reviews and experimental papers present the evidence that glia do indeed have the capacity to respond dynamically to a wide range of stimuli with persistent changes in signal transduction, morphology, and homeostasis. In \" Plasticity of Neuron-Glial Transmission: Equipping Glia for Long-Term Integration of Network Activity, \" W. Croft et al. review the current evidence for plasticity in neuron-glial communication and speculate on the implication of differences in induction paradigms from synaptic plasticity for the computational properties of glial signaling. In \" Glutamatergic Transmission: A Matter of Three, \" Z. Martínez-Lozada and A. Ortega focus on the consequences of glutamate receptor activation for astroglial physiology. By identifying the downstream targets engaged by gluta-matergic signalling, the authors show how neurons can shape transcriptional and translational regulation in glia to tailor transmitter clearance and recycling to meet synaptic demands. Remaining with regulation of intracellular signaling in astrocytes, N. Komin et al. present an analysis of the impact of variation in uptake of calcium into endoplasmic reticulum stores on cytoplasmic calcium oscillations in \" Multiscale Modeling Indicates That Temperature Dependent [Ca 2+ ] i Spiking in Astrocytes Is Quantitatively Consistent with Modulated SERCA Activity. \" The results of the modelling study illustrate the striking sensitivity of intracellular calcium dynamics to changes in SERCA activity with implications both for interpretation of experimental results at nonphys-iological temperatures and for prediction of calcium signal kinetics in vivo. In \" Fractalkine Signaling and Microglia Functions in the Developing Brain, \" I. Arnoux and E. Audinat review the effects of fractalkine receptor activation on microglial function. The review …","PeriodicalId":51299,"journal":{"name":"Neural Plasticity","volume":"2015 ","pages":"723891"},"PeriodicalIF":3.1,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/723891","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33983774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01Epub Date: 2015-05-20DOI: 10.1155/2015/167308
Sylwia Owczarek, Marie Louise Bang, Vladimir Berezin
Neurexins (NXs) and neuroligins (NLs) are cell adhesion molecules that are localized at opposite sites of synaptic membranes. They interact with each other to promote the assembly, maintenance, and function of synapses in the central nervous system. Both NX and NL are cleaved from a membrane-attached intracellular domain in an activity-dependent manner, generating the soluble ectodomain of NX or NL. Expression of the NX1 and NX3 genes in the brain appears to be regulated by a schizophrenia-related protein, DISC1. Here, we show that soluble ecto-NX1β can regulate the expression of DISC1 and induce signaling downstream of DISC1. We also show that NL1 binds to a well-characterized DISC1 interaction partner, Kal-7, and this interaction can be compromised by DISC1. Our results indicate that the NX/NL synaptic complex is intrinsically involved in the regulation of DISC1 function, thus contributing to a better understanding of the pathology of schizophrenia.
{"title":"Neurexin-Neuroligin Synaptic Complex Regulates Schizophrenia-Related DISC1/Kal-7/Rac1 \"Signalosome\".","authors":"Sylwia Owczarek, Marie Louise Bang, Vladimir Berezin","doi":"10.1155/2015/167308","DOIUrl":"https://doi.org/10.1155/2015/167308","url":null,"abstract":"<p><p>Neurexins (NXs) and neuroligins (NLs) are cell adhesion molecules that are localized at opposite sites of synaptic membranes. They interact with each other to promote the assembly, maintenance, and function of synapses in the central nervous system. Both NX and NL are cleaved from a membrane-attached intracellular domain in an activity-dependent manner, generating the soluble ectodomain of NX or NL. Expression of the NX1 and NX3 genes in the brain appears to be regulated by a schizophrenia-related protein, DISC1. Here, we show that soluble ecto-NX1β can regulate the expression of DISC1 and induce signaling downstream of DISC1. We also show that NL1 binds to a well-characterized DISC1 interaction partner, Kal-7, and this interaction can be compromised by DISC1. Our results indicate that the NX/NL synaptic complex is intrinsically involved in the regulation of DISC1 function, thus contributing to a better understanding of the pathology of schizophrenia. </p>","PeriodicalId":51299,"journal":{"name":"Neural Plasticity","volume":"2015 ","pages":"167308"},"PeriodicalIF":3.1,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/167308","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34208830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01Epub Date: 2015-06-10DOI: 10.1155/2015/602356
Katharine L Dobson, Tomas C Bellamy
In the cerebellar molecular layer parallel fibre terminals release glutamate from both the active zone and from extrasynaptic "ectopic" sites. Ectopic release mediates transmission to the Bergmann glia that ensheathe the synapse, activating Ca(2+)-permeable AMPA receptors and glutamate transporters. Parallel fibre terminals exhibit several forms of presynaptic plasticity, including cAMP-dependent long-term potentiation and endocannabinoid-dependent long-term depression, but it is not known whether these presynaptic forms of long-term plasticity also influence ectopic transmission to Bergmann glia. Stimulation of parallel fibre inputs at 16 Hz evoked LTP of synaptic transmission, but LTD of ectopic transmission. Pharmacological activation of adenylyl cyclase by forskolin caused LTP at Purkinje neurons, but only transient potentiation at Bergmann glia, reinforcing the concept that ectopic sites lack the capacity to express sustained cAMP-dependent potentiation. Activation of mGluR1 caused depression of synaptic transmission via retrograde endocannabinoid signalling but had no significant effect at ectopic sites. In contrast, activation of NMDA receptors suppressed both synaptic and ectopic transmission. The results suggest that the signalling mechanisms for presynaptic LTP and retrograde depression by endocannabinoids are restricted to the active zone at parallel fibre synapses, allowing independent modulation of synaptic transmission to Purkinje neurons and ectopic transmission to Bergmann glia.
{"title":"Localization of Presynaptic Plasticity Mechanisms Enables Functional Independence of Synaptic and Ectopic Transmission in the Cerebellum.","authors":"Katharine L Dobson, Tomas C Bellamy","doi":"10.1155/2015/602356","DOIUrl":"https://doi.org/10.1155/2015/602356","url":null,"abstract":"<p><p>In the cerebellar molecular layer parallel fibre terminals release glutamate from both the active zone and from extrasynaptic \"ectopic\" sites. Ectopic release mediates transmission to the Bergmann glia that ensheathe the synapse, activating Ca(2+)-permeable AMPA receptors and glutamate transporters. Parallel fibre terminals exhibit several forms of presynaptic plasticity, including cAMP-dependent long-term potentiation and endocannabinoid-dependent long-term depression, but it is not known whether these presynaptic forms of long-term plasticity also influence ectopic transmission to Bergmann glia. Stimulation of parallel fibre inputs at 16 Hz evoked LTP of synaptic transmission, but LTD of ectopic transmission. Pharmacological activation of adenylyl cyclase by forskolin caused LTP at Purkinje neurons, but only transient potentiation at Bergmann glia, reinforcing the concept that ectopic sites lack the capacity to express sustained cAMP-dependent potentiation. Activation of mGluR1 caused depression of synaptic transmission via retrograde endocannabinoid signalling but had no significant effect at ectopic sites. In contrast, activation of NMDA receptors suppressed both synaptic and ectopic transmission. The results suggest that the signalling mechanisms for presynaptic LTP and retrograde depression by endocannabinoids are restricted to the active zone at parallel fibre synapses, allowing independent modulation of synaptic transmission to Purkinje neurons and ectopic transmission to Bergmann glia. </p>","PeriodicalId":51299,"journal":{"name":"Neural Plasticity","volume":"2015 ","pages":"602356"},"PeriodicalIF":3.1,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/602356","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34283009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}