The purpose of the study was to radiographically evaluate the prevalence of palatogingival groove (PG) in the East Indian population in the maxillary anterior teeth in different genders, its unilateral/bilateral presentation, classified based on its radiographic characteristics, to determine the prevalence of different types, which could help in future treatment planning. The design of the study was a retrospective study. Analysis of 429 maxillary anterior teeth (144 central incisors, 145 lateral incisors, and 139 canines) in 72 cone-beam computed tomography scans was done (31 males and 41 females, mean age 27.3 ± 7.63). Demographic details of patients and characteristics of PG, i.e. location, extension, depth, and type, were recorded. The presence of alveolar bone loss and periapical pathology was noted. An overall prevalence of PG was found to be 2.33% (n = 10), with PG being detected in 2 (1.388%) central incisors, 8 (5.51%) lateral incisors, and 0 (0%) canines. Eight of the patients had a unilateral presence, while one patient reported with bilateral presence, implying a significantly higher predilection of unilateral occurrence (P = 0.02). The prevalence was found to be higher in females (n = 8). The teeth were categorized as either having Type I (6 teeth), Type II (3 teeth), or Type III (1 teeth). Three of the 10 PGs were present in the mesial, six in the mid-palatal, and one in the distal portion of the palatal surface. The prevalence of PG in the maxillary incisors in this cohort is 2.33%. The maxillary lateral incisors are the most affected teeth. Unilateral presentation is more common.
{"title":"Cone-beam computed tomographic evaluation to estimate the prevalence of palatogingival groove in the maxillary anterior teeth and its radiographic characteristics: An institutional retrospective study","authors":"Mousumi Biswas, D. Mazumdar, Binayak Saha, Siddhi Agarwala, Kallol Kumar Saha, Kuntal Chowdhury","doi":"10.4103/jcde.jcde_347_23","DOIUrl":"https://doi.org/10.4103/jcde.jcde_347_23","url":null,"abstract":"\u0000 \u0000 \u0000 The purpose of the study was to radiographically evaluate the prevalence of palatogingival groove (PG) in the East Indian population in the maxillary anterior teeth in different genders, its unilateral/bilateral presentation, classified based on its radiographic characteristics, to determine the prevalence of different types, which could help in future treatment planning.\u0000 \u0000 \u0000 \u0000 The design of the study was a retrospective study.\u0000 \u0000 \u0000 \u0000 Analysis of 429 maxillary anterior teeth (144 central incisors, 145 lateral incisors, and 139 canines) in 72 cone-beam computed tomography scans was done (31 males and 41 females, mean age 27.3 ± 7.63). Demographic details of patients and characteristics of PG, i.e. location, extension, depth, and type, were recorded. The presence of alveolar bone loss and periapical pathology was noted.\u0000 \u0000 \u0000 \u0000 An overall prevalence of PG was found to be 2.33% (n = 10), with PG being detected in 2 (1.388%) central incisors, 8 (5.51%) lateral incisors, and 0 (0%) canines. Eight of the patients had a unilateral presence, while one patient reported with bilateral presence, implying a significantly higher predilection of unilateral occurrence (P = 0.02). The prevalence was found to be higher in females (n = 8). The teeth were categorized as either having Type I (6 teeth), Type II (3 teeth), or Type III (1 teeth). Three of the 10 PGs were present in the mesial, six in the mid-palatal, and one in the distal portion of the palatal surface.\u0000 \u0000 \u0000 \u0000 The prevalence of PG in the maxillary incisors in this cohort is 2.33%. The maxillary lateral incisors are the most affected teeth. Unilateral presentation is more common.\u0000","PeriodicalId":516572,"journal":{"name":"Journal of Conservative Dentistry and Endodontics","volume":"8 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140272536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.4103/jcde.jcde_295_23
E. Forabosco, L. Generali, E. Mancuso, S. Kaleci, Ugo Consolo, V. Checchi
Restorative dentistry aims to reproduce natural tooth shades through resin composites that must be layered to obtain colors, opacities, and translucencies, and therefore, clinical success is operator skill dependent. The purpose of this study is to evaluate the color shift of single-shade composite restorations before and after dental bleaching. Eighty human extracted posterior teeth were restored with four single-shade composites (Omnichroma OM; Clearfil Majesty ES-2 Universal CL; Essentia Universal ES; Venus Diamond One VE) (n = 20 each). Standardized V class cavities were prepared on buccal side. VITA Easyshade V spectrophotometer was used to register VITA color and color coordinates 24 h before (T0), 24 h after (T1), and 1 week after (T2) dental bleaching (Opalescence Boost PF 40%). Color differences (ΔEab) and (ΔWID) were calculated and subjected to statistical analysis. Categorical variables were analyzed using Pearson Chi-square, and data from color coordinates were analyzed using one-way analysis of variance and Tukey’s multiple comparison test with Bonferroni correction. Paired t-tests were performed to compare continuous measures between groups and treatment time. Instrumental evaluations revealed statistically significant differences between materials (P < 0.05) with lower values for ES and VE samples followed by CL and OM at T0 and T1. At T2, OM and CL ΔEab values decrease getting closer to ES and VE. Single-shade composites seem to match with the surrounding bleached tooth.
牙科修复旨在通过树脂复合材料再现自然牙色,而树脂复合材料必须分层才能获得颜色、透明度和半透明度,因此临床成功与否取决于操作者的技术。 本研究的目的是评估牙齿漂白前后单色复合树脂修复体的色移。 用四种单色复合树脂(Omnichroma OM;Clearfil Majesty ES-2 Universal CL;Essentia Universal ES;Venus Diamond One VE)修复了 80 颗人类拔出的后牙(每种 20 人)。在颊侧制备了标准化的 V 级腔。使用 VITA Easyshade V 分光光度计记录牙齿漂白(Opalescence Boost PF 40%)前 24 小时(T0)、漂白后 24 小时(T1)和漂白后一周(T2)的 VITA 颜色和颜色坐标。计算色差 (ΔEab) 和 (ΔWID) 并进行统计分析。 分类变量采用皮尔逊卡方进行分析,色坐标数据采用单因素方差分析和带 Bonferroni 校正的 Tukey 多重比较检验进行分析。采用配对 t 检验来比较不同组别和治疗时间的连续性指标。 仪器评估显示,不同材料之间存在显著的统计学差异(P < 0.05),在 T0 和 T1,ES 和 VE 样品的值较低,其次是 CL 和 OM。在 T2,OM 和 CL ΔEab 值下降,越来越接近 ES 和 VE。 单色调复合材料似乎与周围的漂白牙齿相匹配。
{"title":"Color match of single-shade restorations after professional dental bleaching: An in vitro study","authors":"E. Forabosco, L. Generali, E. Mancuso, S. Kaleci, Ugo Consolo, V. Checchi","doi":"10.4103/jcde.jcde_295_23","DOIUrl":"https://doi.org/10.4103/jcde.jcde_295_23","url":null,"abstract":"\u0000 \u0000 \u0000 Restorative dentistry aims to reproduce natural tooth shades through resin composites that must be layered to obtain colors, opacities, and translucencies, and therefore, clinical success is operator skill dependent.\u0000 \u0000 \u0000 \u0000 The purpose of this study is to evaluate the color shift of single-shade composite restorations before and after dental bleaching.\u0000 \u0000 \u0000 \u0000 Eighty human extracted posterior teeth were restored with four single-shade composites (Omnichroma OM; Clearfil Majesty ES-2 Universal CL; Essentia Universal ES; Venus Diamond One VE) (n = 20 each). Standardized V class cavities were prepared on buccal side. VITA Easyshade V spectrophotometer was used to register VITA color and color coordinates 24 h before (T0), 24 h after (T1), and 1 week after (T2) dental bleaching (Opalescence Boost PF 40%). Color differences (ΔEab) and (ΔWID) were calculated and subjected to statistical analysis.\u0000 \u0000 \u0000 \u0000 Categorical variables were analyzed using Pearson Chi-square, and data from color coordinates were analyzed using one-way analysis of variance and Tukey’s multiple comparison test with Bonferroni correction. Paired t-tests were performed to compare continuous measures between groups and treatment time.\u0000 \u0000 \u0000 \u0000 Instrumental evaluations revealed statistically significant differences between materials (P < 0.05) with lower values for ES and VE samples followed by CL and OM at T0 and T1. At T2, OM and CL ΔEab values decrease getting closer to ES and VE.\u0000 \u0000 \u0000 \u0000 Single-shade composites seem to match with the surrounding bleached tooth.\u0000","PeriodicalId":516572,"journal":{"name":"Journal of Conservative Dentistry and Endodontics","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140086448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.4103/jcde.jcde_272_23
Vedamani Chandolu, Jyothi Mandava, Roopesh Borugadda, K. Sirisha, Konagala Ravi Kumar, Sameera Goteti, Lalitha Sri Roja Nallamilli
Several designs of access cavity have been evolved in the recent past with the concept of minimal tooth tissue removal which would improve the root canal treated teeth fracture resistance. To investigate the effect of conservative design access cavity during the instrumentation of maxillary molar root canals. Eighty noncarious maxillary molars were assigned to the traditional and conservative access groups (n = 40 each). After designated access preparations, the teeth were immersed in Lugols’s solution for staining the pulp tissue. Root canal instrumentation was performed with TruNatomy file system. Pre- and postinstrumentation nano-computed tomography (CT) images were taken and reconstructed using CT-An software. Root canals volumetric analysis was done with CT-Vol software. The analysis of the data was dealt with Shapiro–Wilk test and independent t-test. The volume of pulp canal space before and after instrumentation changed significantly between the traditional and conservative access design groups, according to an independent t-test. In comparison, the mean volume of dentin removed was much larger in the TAC group (P = 0.0016). The independent t-test manifests difference significantly between traditional endodontic access cavity (TAC) and conservative access cavity (CAC) with percentage of unprepared canal walls. The mean percentage of unprepared area was significantly lesser in TAC group as compared to CAC group (P = 0.0022). The volume of dentin removed was greater in TAC than with the CAC design. The amount of untouched canal wall area was significantly higher in conservative access design than with the traditional access design group.
{"title":"Influence of access cavity design on root canal instrumentation efficacy in molars – An in vitro study","authors":"Vedamani Chandolu, Jyothi Mandava, Roopesh Borugadda, K. Sirisha, Konagala Ravi Kumar, Sameera Goteti, Lalitha Sri Roja Nallamilli","doi":"10.4103/jcde.jcde_272_23","DOIUrl":"https://doi.org/10.4103/jcde.jcde_272_23","url":null,"abstract":"\u0000 \u0000 \u0000 Several designs of access cavity have been evolved in the recent past with the concept of minimal tooth tissue removal which would improve the root canal treated teeth fracture resistance.\u0000 \u0000 \u0000 \u0000 To investigate the effect of conservative design access cavity during the instrumentation of maxillary molar root canals.\u0000 \u0000 \u0000 \u0000 Eighty noncarious maxillary molars were assigned to the traditional and conservative access groups (n = 40 each). After designated access preparations, the teeth were immersed in Lugols’s solution for staining the pulp tissue. Root canal instrumentation was performed with TruNatomy file system. Pre- and postinstrumentation nano-computed tomography (CT) images were taken and reconstructed using CT-An software. Root canals volumetric analysis was done with CT-Vol software. The analysis of the data was dealt with Shapiro–Wilk test and independent t-test.\u0000 \u0000 \u0000 \u0000 The volume of pulp canal space before and after instrumentation changed significantly between the traditional and conservative access design groups, according to an independent t-test. In comparison, the mean volume of dentin removed was much larger in the TAC group (P = 0.0016). The independent t-test manifests difference significantly between traditional endodontic access cavity (TAC) and conservative access cavity (CAC) with percentage of unprepared canal walls. The mean percentage of unprepared area was significantly lesser in TAC group as compared to CAC group (P = 0.0022).\u0000 \u0000 \u0000 \u0000 The volume of dentin removed was greater in TAC than with the CAC design. The amount of untouched canal wall area was significantly higher in conservative access design than with the traditional access design group.\u0000","PeriodicalId":516572,"journal":{"name":"Journal of Conservative Dentistry and Endodontics","volume":"114 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140090874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cytotoxicity and adaptability are among the highly imperative tests that should be performed on a novel endodontic material to ensure its successful implementation in endodontic treatment. Assess a recently introduced bioceramic root canal sealer CeramoSeal with TotalFill BC and AH plus sealers regarding the cytotoxicity and adaptability. Five sealer discs were prepared for each sealer and their extracts were cultured in 96-well plates containing human fibroblasts for 24 h. After their incubation, MTT solution was added to each well plate using an enzyme-linked immunosorbent assay plate reader was implemented to calculate the percentage of viable cells. Thirty mandibular single-rooted premolars were prepared using the Edge Endo rotary system, teeth were divided into three groups (n = 10) based on the sealer type: Group 1 CeramoSeal, Group 2 Totalfill, and Group 3 AH plus sealer. Teeth were sectioned longitudinally and viewed under a scanning electron microscope where the region with the gaps was identified and quantified as a percentage of the root canal’s overall area. One-way ANOVA test was used for cytotoxicity, while Kruskal–Wallis and Friedman’s tests were used for adaptability. Ceramoseal statistically significantly showed the lowest viability, at high concentrations AH plus showed the highest cell viability, while at lower concentration Totalfill BC sealer showed the highest cell viability percentage. The gap percentages were statistically significantly higher in Ceramoseal group, there was no statistically significant difference between AH Plus and Totalfill groups. Ceramoseal sealer exhibited the lowest viability and highest gap percentage compared to the other sealers.
{"title":"Evaluation of cytotoxicity and adaptability of a novel bioceramic root canal sealer: An in vitro and scanning electron microscope study","authors":"Soha Alaa Emam, A. Mahran, M. Elshafei","doi":"10.4103/jcde.jcde_40_24","DOIUrl":"https://doi.org/10.4103/jcde.jcde_40_24","url":null,"abstract":"\u0000 \u0000 \u0000 Cytotoxicity and adaptability are among the highly imperative tests that should be performed on a novel endodontic material to ensure its successful implementation in endodontic treatment.\u0000 \u0000 \u0000 \u0000 Assess a recently introduced bioceramic root canal sealer CeramoSeal with TotalFill BC and AH plus sealers regarding the cytotoxicity and adaptability.\u0000 \u0000 \u0000 \u0000 Five sealer discs were prepared for each sealer and their extracts were cultured in 96-well plates containing human fibroblasts for 24 h. After their incubation, MTT solution was added to each well plate using an enzyme-linked immunosorbent assay plate reader was implemented to calculate the percentage of viable cells. Thirty mandibular single-rooted premolars were prepared using the Edge Endo rotary system, teeth were divided into three groups (n = 10) based on the sealer type: Group 1 CeramoSeal, Group 2 Totalfill, and Group 3 AH plus sealer. Teeth were sectioned longitudinally and viewed under a scanning electron microscope where the region with the gaps was identified and quantified as a percentage of the root canal’s overall area.\u0000 \u0000 \u0000 \u0000 One-way ANOVA test was used for cytotoxicity, while Kruskal–Wallis and Friedman’s tests were used for adaptability.\u0000 \u0000 \u0000 \u0000 Ceramoseal statistically significantly showed the lowest viability, at high concentrations AH plus showed the highest cell viability, while at lower concentration Totalfill BC sealer showed the highest cell viability percentage. The gap percentages were statistically significantly higher in Ceramoseal group, there was no statistically significant difference between AH Plus and Totalfill groups.\u0000 \u0000 \u0000 \u0000 Ceramoseal sealer exhibited the lowest viability and highest gap percentage compared to the other sealers.\u0000","PeriodicalId":516572,"journal":{"name":"Journal of Conservative Dentistry and Endodontics","volume":"111 51","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140088747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.4103/jcde.jcde_310_23
Sowmya Kavoor, M. Ranjini, Naval Abdul Aziz, H. K. Ashok, Roopa R. Nadig
Teeth bleaching, although considered safe and conservative, cause microscopic changes in the tooth structure. The aim of this study is to evaluate the bleaching efficacy of carbamide peroxide (CP) bleaching gel when modified with the incorporation of bioactive glass (BG) and hydroxyapatite (HA) and its effect on enamel microhardness. Forty-five maxillary incisors were decoronated, artificially stained and mounted in acrylic. The samples were divided into three groups of 15 each and subjected to the following bleaching protocol for 8 h/day at 37°C for 2 weeks: Group 1 – 16% CP, Group 2 – CP modified with BG, and Group 3 – CP modified with hydroxyapatite (HA). Spectrophotometric color assessment using CIE L*a*b* system and Vickers microhardness were assessed before and after bleaching. Data were analyzed using Student’s paired t-test and one-way ANOVA followed by Tukey’s post hoc analysis. There was a significant change in color (L*a*b*) in all the three groups when compared to the baseline values. However, no significant difference in the total color change (ΔE) was observed between the three groups. Enamel microhardness reduced significantly in the CP group, whereas it increased in the BG and HA group after bleaching. Scanning electron microscopy images of BG and HA groups showed crystalline deposits suggesting mineral deposition. Addition of biomaterials can be a beneficial alternative to bleaching with CP alone, considering the increase in microhardness without hindering the bleaching action.
{"title":"In vitro evaluation of the effect of addition of biomaterials to carbamide peroxide on the bleaching efficacy and microhardness of enamel","authors":"Sowmya Kavoor, M. Ranjini, Naval Abdul Aziz, H. K. Ashok, Roopa R. Nadig","doi":"10.4103/jcde.jcde_310_23","DOIUrl":"https://doi.org/10.4103/jcde.jcde_310_23","url":null,"abstract":"\u0000 \u0000 \u0000 Teeth bleaching, although considered safe and conservative, cause microscopic changes in the tooth structure. The aim of this study is to evaluate the bleaching efficacy of carbamide peroxide (CP) bleaching gel when modified with the incorporation of bioactive glass (BG) and hydroxyapatite (HA) and its effect on enamel microhardness.\u0000 \u0000 \u0000 \u0000 Forty-five maxillary incisors were decoronated, artificially stained and mounted in acrylic. The samples were divided into three groups of 15 each and subjected to the following bleaching protocol for 8 h/day at 37°C for 2 weeks: Group 1 – 16% CP, Group 2 – CP modified with BG, and Group 3 – CP modified with hydroxyapatite (HA). Spectrophotometric color assessment using CIE L*a*b* system and Vickers microhardness were assessed before and after bleaching. Data were analyzed using Student’s paired t-test and one-way ANOVA followed by Tukey’s post hoc analysis.\u0000 \u0000 \u0000 \u0000 There was a significant change in color (L*a*b*) in all the three groups when compared to the baseline values. However, no significant difference in the total color change (ΔE) was observed between the three groups. Enamel microhardness reduced significantly in the CP group, whereas it increased in the BG and HA group after bleaching. Scanning electron microscopy images of BG and HA groups showed crystalline deposits suggesting mineral deposition.\u0000 \u0000 \u0000 \u0000 Addition of biomaterials can be a beneficial alternative to bleaching with CP alone, considering the increase in microhardness without hindering the bleaching action.\u0000","PeriodicalId":516572,"journal":{"name":"Journal of Conservative Dentistry and Endodontics","volume":"10 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140090943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.4103/jcde.jcde_266_23
S. Aarthi, J. S. Sivakumar, A. Sivakumar, J. S. Soundappan, M. Chittrarasu, G. Jayanthi
Safe and efficient removal of all root filling materials from the root canal system without compromising radicular dentin structure is essential for optimal nonsurgical retreatment. The aim of this study was to evaluate and compare the incidence of dentinal defects caused during root canal filling removal using conventional, rotary, and reciprocating retreatment file systems. A detailed protocol explaining purpose and procedures of the study was submitted to the Institutional Ethics Committee and ethical clearance obtained. Sixty human maxillary permanent central incisors were collected and decoronated to 12-mm standardized length. The canals prepared up to a master apical file size F3 with Protaper hand files, obturated using AH plus sealer, examined under the stereomicroscope (×40 magnification): Group I: Control (n = 15), Group II: Conventional (n = 15), Group III: Protaper Universal Retreatment Files (n = 15), and Group IV: Reciproc Blue (n = 15). After instrumentation, teeth were sectioned at 3, 6, and 9 mm from the apex to evaluate the presence of dentinal defects under the stereomicroscope. Statistics were performed using the SPSS, version, 25 (SPSS Inc., Chicago, IL, USA). Initially, normality test was done using the Shapiro–Wilk test and data were not normally distributed followed by Kruskal–Wallis test. P < 0.05 is considered statistically significant. Maximum percentage increase in dentinal defects was observed in Protaper Universal Retreatment Files followed by Conventional method and Reciproc Blue. Significantly Reciproc Blue reduced the incidence of dentinal defects after root canal preparation.
{"title":"Comparative evaluation of incidence of dentinal defects after root canal preparation using three different endodontic retreatment systems – An in vitro study","authors":"S. Aarthi, J. S. Sivakumar, A. Sivakumar, J. S. Soundappan, M. Chittrarasu, G. Jayanthi","doi":"10.4103/jcde.jcde_266_23","DOIUrl":"https://doi.org/10.4103/jcde.jcde_266_23","url":null,"abstract":"\u0000 \u0000 \u0000 Safe and efficient removal of all root filling materials from the root canal system without compromising radicular dentin structure is essential for optimal nonsurgical retreatment.\u0000 \u0000 \u0000 \u0000 The aim of this study was to evaluate and compare the incidence of dentinal defects caused during root canal filling removal using conventional, rotary, and reciprocating retreatment file systems.\u0000 \u0000 \u0000 \u0000 A detailed protocol explaining purpose and procedures of the study was submitted to the Institutional Ethics Committee and ethical clearance obtained.\u0000 \u0000 \u0000 \u0000 Sixty human maxillary permanent central incisors were collected and decoronated to 12-mm standardized length. The canals prepared up to a master apical file size F3 with Protaper hand files, obturated using AH plus sealer, examined under the stereomicroscope (×40 magnification): Group I: Control (n = 15), Group II: Conventional (n = 15), Group III: Protaper Universal Retreatment Files (n = 15), and Group IV: Reciproc Blue (n = 15). After instrumentation, teeth were sectioned at 3, 6, and 9 mm from the apex to evaluate the presence of dentinal defects under the stereomicroscope.\u0000 \u0000 \u0000 \u0000 Statistics were performed using the SPSS, version, 25 (SPSS Inc., Chicago, IL, USA). Initially, normality test was done using the Shapiro–Wilk test and data were not normally distributed followed by Kruskal–Wallis test. P < 0.05 is considered statistically significant.\u0000 \u0000 \u0000 \u0000 Maximum percentage increase in dentinal defects was observed in Protaper Universal Retreatment Files followed by Conventional method and Reciproc Blue.\u0000 \u0000 \u0000 \u0000 Significantly Reciproc Blue reduced the incidence of dentinal defects after root canal preparation.\u0000","PeriodicalId":516572,"journal":{"name":"Journal of Conservative Dentistry and Endodontics","volume":"26 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140268206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nanorevolution: Trends and future perspectives","authors":"Shishir Singh, S. Saha","doi":"10.4103/jcde.jcde_71_24","DOIUrl":"https://doi.org/10.4103/jcde.jcde_71_24","url":null,"abstract":"","PeriodicalId":516572,"journal":{"name":"Journal of Conservative Dentistry and Endodontics","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140083277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.4103/jcde.jcde_234_23
Vivek Devidas Mahale, Sonali Sharma
The objective of this study was to ascertain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of royal jelly (RJ) against three microorganisms frequently linked with endodontic infections: Staphylococcus aureus, Enterococcus faecalis, and Candida albicans. Freshly harvested RJ was prepared at different concentrations (20%, 10%, 5%, 2.5%, and 1.25%) in distilled water. The microbial cultures of the target organisms were prepared. MIC was determined using a broth dilution technique, monitoring microbial growth. MBC was determined by inoculating agar plates with samples from tubes showing no apparent growth and evaluating the presence of bacterial or fungal growth following the incubation period. For S. aureus, the MIC and MBC were 5 mg/ml of RJ. For E. faecalis, the MIC and MBC were 10 mg/ml of RJ. For C. albicans, both MIC and MBC were 10 mg/ml of RJ. The findings demonstrated RJ’s potential to inhibit and eliminate these pathogenic microorganisms, making it a potential candidate for endodontic infection control. The antimicrobial properties of RJ against S. aureus, E. faecalis, and C. albicans present a promising avenue for enhancing infection control in endodontics. Additional investigations are needed to refine its use in clinical settings, especially in cases with mixed microbial infections.
这项研究的目的是确定蜂王浆(RJ)对三种经常与牙髓感染有关的微生物的最低抑菌浓度(MIC)和最低杀菌浓度(MBC):金黄色葡萄球菌、粪肠球菌和白色念珠菌。 在蒸馏水中配制不同浓度(20%、10%、5%、2.5% 和 1.25%)的新鲜采摘的 RJ。制备目标生物的微生物培养物。使用肉汤稀释技术测定 MIC,监测微生物生长。在琼脂平板上接种无明显生长的试管样品,并在培养期后评估是否有细菌或真菌生长,从而确定 MBC。 金黄色葡萄球菌的 MIC 和 MBC 均为 5 毫克/毫升 RJ。对于粪肠球菌,RJ 的 MIC 和 MBC 均为 10 毫克/毫升。对于白僵菌,RJ 的 MIC 和 MBC 均为 10 毫克/毫升。研究结果表明,RJ 具有抑制和消除这些病原微生物的潜力,使其成为牙髓感染控制的潜在候选药物。 RJ 对金黄色葡萄球菌、粪大肠杆菌和白念珠菌的抗菌特性为加强牙髓病的感染控制提供了一个前景广阔的途径。还需要进行更多的研究来完善其在临床环境中的应用,尤其是在混合微生物感染的病例中。
{"title":"Evaluation of minimum inhibitory concentration and minimum bactericidal concentration of royal jelly against Enterococcus faecalis, Staphylococcus aureus, and Candida albicans","authors":"Vivek Devidas Mahale, Sonali Sharma","doi":"10.4103/jcde.jcde_234_23","DOIUrl":"https://doi.org/10.4103/jcde.jcde_234_23","url":null,"abstract":"\u0000 \u0000 \u0000 The objective of this study was to ascertain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of royal jelly (RJ) against three microorganisms frequently linked with endodontic infections: Staphylococcus aureus, Enterococcus faecalis, and Candida albicans.\u0000 \u0000 \u0000 \u0000 Freshly harvested RJ was prepared at different concentrations (20%, 10%, 5%, 2.5%, and 1.25%) in distilled water. The microbial cultures of the target organisms were prepared. MIC was determined using a broth dilution technique, monitoring microbial growth. MBC was determined by inoculating agar plates with samples from tubes showing no apparent growth and evaluating the presence of bacterial or fungal growth following the incubation period.\u0000 \u0000 \u0000 \u0000 For S. aureus, the MIC and MBC were 5 mg/ml of RJ. For E. faecalis, the MIC and MBC were 10 mg/ml of RJ. For C. albicans, both MIC and MBC were 10 mg/ml of RJ. The findings demonstrated RJ’s potential to inhibit and eliminate these pathogenic microorganisms, making it a potential candidate for endodontic infection control.\u0000 \u0000 \u0000 \u0000 The antimicrobial properties of RJ against S. aureus, E. faecalis, and C. albicans present a promising avenue for enhancing infection control in endodontics. Additional investigations are needed to refine its use in clinical settings, especially in cases with mixed microbial infections.\u0000","PeriodicalId":516572,"journal":{"name":"Journal of Conservative Dentistry and Endodontics","volume":"113 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140088543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.4103/jcde.jcde_137_23
L. S. Alves, F. Ormiga, C. D. de Lima, R. Lopes, H. Gusman
Complementary procedures have been proposed to improve the endodontic retreatments. The aim of this study was to evaluate, by microcomputed tomography (micro-CT), the dentin wear caused by passive ultrasonic irrigation (PUI) and easy clean (EC) instruments, when used for the final removal of filling material during endodontic retreatment. Thirty-six mesial roots of the lower first molars were divided into four groups (n = 9), according to the final irrigation and sealer: PUI/AH Plus, EC/AH Plus, PUI/Total Fill (TF), and EC/TF. Canal volume was evaluated, both before and after the final irrigation, by micro-CT analysis. The Kruskal–Wallis test was used to compare the volumes among groups and the paired Wilcoxon test was used to compare the volume values before and after the final irrigation within each group. Both complementary cleaning procedures promoted dentin wear that was observed only when the volume was analyzed for each third of the root canal. In the apical third, both instruments showed significant wear, where the use of the EC instrument resulted in greater wear than the PUI, in teeth filled with both AH Plus and TF BC Sealers (P < 0.05). Both complementary cleaning procedures promoted dentin wear and must be used cautiously.
{"title":"Root canal dentin wear during final irrigation in endodontic retreatment with passive ultrasonic irrigation and easy clean instruments","authors":"L. S. Alves, F. Ormiga, C. D. de Lima, R. Lopes, H. Gusman","doi":"10.4103/jcde.jcde_137_23","DOIUrl":"https://doi.org/10.4103/jcde.jcde_137_23","url":null,"abstract":"\u0000 \u0000 \u0000 Complementary procedures have been proposed to improve the endodontic retreatments.\u0000 \u0000 \u0000 \u0000 The aim of this study was to evaluate, by microcomputed tomography (micro-CT), the dentin wear caused by passive ultrasonic irrigation (PUI) and easy clean (EC) instruments, when used for the final removal of filling material during endodontic retreatment.\u0000 \u0000 \u0000 \u0000 Thirty-six mesial roots of the lower first molars were divided into four groups (n = 9), according to the final irrigation and sealer: PUI/AH Plus, EC/AH Plus, PUI/Total Fill (TF), and EC/TF. Canal volume was evaluated, both before and after the final irrigation, by micro-CT analysis. The Kruskal–Wallis test was used to compare the volumes among groups and the paired Wilcoxon test was used to compare the volume values before and after the final irrigation within each group.\u0000 \u0000 \u0000 \u0000 Both complementary cleaning procedures promoted dentin wear that was observed only when the volume was analyzed for each third of the root canal. In the apical third, both instruments showed significant wear, where the use of the EC instrument resulted in greater wear than the PUI, in teeth filled with both AH Plus and TF BC Sealers (P < 0.05).\u0000 \u0000 \u0000 \u0000 Both complementary cleaning procedures promoted dentin wear and must be used cautiously.\u0000","PeriodicalId":516572,"journal":{"name":"Journal of Conservative Dentistry and Endodontics","volume":"105 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140274470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.4103/jcde.jcde_265_23
Yantrapragada Lakshmi Sunanda, K. Parvathaneni, T. Raju, Abitha Seshadri, Nadimpalli Mahendra Varma, G. Dondapati
The aim of the study is to evaluate the penetration depth of irrigants mixed with NaOCl into dentinal tubules at the apical third of the root canal using a confocal laser scanning microscope (CLSM). Thirty-six single-rooted teeth were selected, decoronated, and straight-line access established. Then, the canal was enlarged with hand Protaper till size F3. The samples were divided randomly into three groups: Group 1 was irrigated with 3% NaOCl mixed with 1% phytic acid; Group 2 was irrigated with 3% NaOCl mixed with 18% etidronic acid; and Group 3 was irrigated with 3% NaOCl mixed with chitosan. The samples were subjected to CLSM evaluation. One-way analysis of variance with Tukey’s post hoc was used for statistical analysis. Group 3 (NaOCl with chitosan) showed a greater depth of penetration in comparison to Groups 1 and 2. Within limitations, it can be concluded that chitosan, in combination with NaOCl can be used as an alternative to the current irrigation protocol.
{"title":"Is a mix – A fix? “A microscopic analysis of depth of penetration of three combinations of irrigants”","authors":"Yantrapragada Lakshmi Sunanda, K. Parvathaneni, T. Raju, Abitha Seshadri, Nadimpalli Mahendra Varma, G. Dondapati","doi":"10.4103/jcde.jcde_265_23","DOIUrl":"https://doi.org/10.4103/jcde.jcde_265_23","url":null,"abstract":"\u0000 \u0000 \u0000 The aim of the study is to evaluate the penetration depth of irrigants mixed with NaOCl into dentinal tubules at the apical third of the root canal using a confocal laser scanning microscope (CLSM).\u0000 \u0000 \u0000 \u0000 Thirty-six single-rooted teeth were selected, decoronated, and straight-line access established. Then, the canal was enlarged with hand Protaper till size F3. The samples were divided randomly into three groups: Group 1 was irrigated with 3% NaOCl mixed with 1% phytic acid; Group 2 was irrigated with 3% NaOCl mixed with 18% etidronic acid; and Group 3 was irrigated with 3% NaOCl mixed with chitosan. The samples were subjected to CLSM evaluation. One-way analysis of variance with Tukey’s post hoc was used for statistical analysis.\u0000 \u0000 \u0000 \u0000 Group 3 (NaOCl with chitosan) showed a greater depth of penetration in comparison to Groups 1 and 2.\u0000 \u0000 \u0000 \u0000 Within limitations, it can be concluded that chitosan, in combination with NaOCl can be used as an alternative to the current irrigation protocol.\u0000","PeriodicalId":516572,"journal":{"name":"Journal of Conservative Dentistry and Endodontics","volume":"12 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139817387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}