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Cone-beam computed tomographic evaluation to estimate the prevalence of palatogingival groove in the maxillary anterior teeth and its radiographic characteristics: An institutional retrospective study 通过锥形束计算机断层扫描评估上颌前牙腭龈沟的患病率及其放射学特征:一项机构回顾性研究
Pub Date : 2024-03-01 DOI: 10.4103/jcde.jcde_347_23
Mousumi Biswas, D. Mazumdar, Binayak Saha, Siddhi Agarwala, Kallol Kumar Saha, Kuntal Chowdhury
The purpose of the study was to radiographically evaluate the prevalence of palatogingival groove (PG) in the East Indian population in the maxillary anterior teeth in different genders, its unilateral/bilateral presentation, classified based on its radiographic characteristics, to determine the prevalence of different types, which could help in future treatment planning. The design of the study was a retrospective study. Analysis of 429 maxillary anterior teeth (144 central incisors, 145 lateral incisors, and 139 canines) in 72 cone-beam computed tomography scans was done (31 males and 41 females, mean age 27.3 ± 7.63). Demographic details of patients and characteristics of PG, i.e. location, extension, depth, and type, were recorded. The presence of alveolar bone loss and periapical pathology was noted. An overall prevalence of PG was found to be 2.33% (n = 10), with PG being detected in 2 (1.388%) central incisors, 8 (5.51%) lateral incisors, and 0 (0%) canines. Eight of the patients had a unilateral presence, while one patient reported with bilateral presence, implying a significantly higher predilection of unilateral occurrence (P = 0.02). The prevalence was found to be higher in females (n = 8). The teeth were categorized as either having Type I (6 teeth), Type II (3 teeth), or Type III (1 teeth). Three of the 10 PGs were present in the mesial, six in the mid-palatal, and one in the distal portion of the palatal surface. The prevalence of PG in the maxillary incisors in this cohort is 2.33%. The maxillary lateral incisors are the most affected teeth. Unilateral presentation is more common.
本研究的目的是对东印度人群不同性别上颌前牙腭龈沟(PG)的患病率、单侧/双侧表现进行放射学评估,并根据其放射学特征进行分类,以确定不同类型的患病率,从而有助于未来的治疗规划。 研究设计为回顾性研究。 研究分析了 72 次锥形束计算机断层扫描中的 429 颗上颌前牙(144 颗中切牙、145 颗侧切牙和 139 颗犬牙)(男性 31 人,女性 41 人,平均年龄(27.3±7.63)岁)。记录了患者的详细人口统计学资料和 PG 的特征,即位置、延伸、深度和类型。同时还记录了牙槽骨缺损和根尖周病变的情况。 结果发现,PG的总患病率为2.33%(n = 10),其中2颗(1.388%)中切牙、8颗(5.51%)侧切牙和0颗(0%)犬齿被检测出PG。其中 8 名患者为单侧发病,1 名患者为双侧发病,这意味着单侧发病率明显更高(P = 0.02)。女性的发病率更高(n = 8)。牙齿被分为 I 型(6 颗)、II 型(3 颗)或 III 型(1 颗)。10 颗 PG 中,3 颗位于腭中面,6 颗位于腭中面,1 颗位于腭面远端。 在该组人群中,上颌切牙的 PG 患病率为 2.33%。上颌侧切牙是发病率最高的牙齿。单侧发病更为常见。
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引用次数: 0
Color match of single-shade restorations after professional dental bleaching: An in vitro study 专业牙齿漂白后单一色调修复体的颜色匹配:体外研究
Pub Date : 2024-03-01 DOI: 10.4103/jcde.jcde_295_23
E. Forabosco, L. Generali, E. Mancuso, S. Kaleci, Ugo Consolo, V. Checchi
Restorative dentistry aims to reproduce natural tooth shades through resin composites that must be layered to obtain colors, opacities, and translucencies, and therefore, clinical success is operator skill dependent. The purpose of this study is to evaluate the color shift of single-shade composite restorations before and after dental bleaching. Eighty human extracted posterior teeth were restored with four single-shade composites (Omnichroma OM; Clearfil Majesty ES-2 Universal CL; Essentia Universal ES; Venus Diamond One VE) (n = 20 each). Standardized V class cavities were prepared on buccal side. VITA Easyshade V spectrophotometer was used to register VITA color and color coordinates 24 h before (T0), 24 h after (T1), and 1 week after (T2) dental bleaching (Opalescence Boost PF 40%). Color differences (ΔEab) and (ΔWID) were calculated and subjected to statistical analysis. Categorical variables were analyzed using Pearson Chi-square, and data from color coordinates were analyzed using one-way analysis of variance and Tukey’s multiple comparison test with Bonferroni correction. Paired t-tests were performed to compare continuous measures between groups and treatment time. Instrumental evaluations revealed statistically significant differences between materials (P < 0.05) with lower values for ES and VE samples followed by CL and OM at T0 and T1. At T2, OM and CL ΔEab values decrease getting closer to ES and VE. Single-shade composites seem to match with the surrounding bleached tooth.
牙科修复旨在通过树脂复合材料再现自然牙色,而树脂复合材料必须分层才能获得颜色、透明度和半透明度,因此临床成功与否取决于操作者的技术。 本研究的目的是评估牙齿漂白前后单色复合树脂修复体的色移。 用四种单色复合树脂(Omnichroma OM;Clearfil Majesty ES-2 Universal CL;Essentia Universal ES;Venus Diamond One VE)修复了 80 颗人类拔出的后牙(每种 20 人)。在颊侧制备了标准化的 V 级腔。使用 VITA Easyshade V 分光光度计记录牙齿漂白(Opalescence Boost PF 40%)前 24 小时(T0)、漂白后 24 小时(T1)和漂白后一周(T2)的 VITA 颜色和颜色坐标。计算色差 (ΔEab) 和 (ΔWID) 并进行统计分析。 分类变量采用皮尔逊卡方进行分析,色坐标数据采用单因素方差分析和带 Bonferroni 校正的 Tukey 多重比较检验进行分析。采用配对 t 检验来比较不同组别和治疗时间的连续性指标。 仪器评估显示,不同材料之间存在显著的统计学差异(P < 0.05),在 T0 和 T1,ES 和 VE 样品的值较低,其次是 CL 和 OM。在 T2,OM 和 CL ΔEab 值下降,越来越接近 ES 和 VE。 单色调复合材料似乎与周围的漂白牙齿相匹配。
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引用次数: 0
Influence of access cavity design on root canal instrumentation efficacy in molars – An in vitro study 接入腔设计对磨牙根管器械疗效的影响 - 体外研究
Pub Date : 2024-03-01 DOI: 10.4103/jcde.jcde_272_23
Vedamani Chandolu, Jyothi Mandava, Roopesh Borugadda, K. Sirisha, Konagala Ravi Kumar, Sameera Goteti, Lalitha Sri Roja Nallamilli
Several designs of access cavity have been evolved in the recent past with the concept of minimal tooth tissue removal which would improve the root canal treated teeth fracture resistance. To investigate the effect of conservative design access cavity during the instrumentation of maxillary molar root canals. Eighty noncarious maxillary molars were assigned to the traditional and conservative access groups (n = 40 each). After designated access preparations, the teeth were immersed in Lugols’s solution for staining the pulp tissue. Root canal instrumentation was performed with TruNatomy file system. Pre- and postinstrumentation nano-computed tomography (CT) images were taken and reconstructed using CT-An software. Root canals volumetric analysis was done with CT-Vol software. The analysis of the data was dealt with Shapiro–Wilk test and independent t-test. The volume of pulp canal space before and after instrumentation changed significantly between the traditional and conservative access design groups, according to an independent t-test. In comparison, the mean volume of dentin removed was much larger in the TAC group (P = 0.0016). The independent t-test manifests difference significantly between traditional endodontic access cavity (TAC) and conservative access cavity (CAC) with percentage of unprepared canal walls. The mean percentage of unprepared area was significantly lesser in TAC group as compared to CAC group (P = 0.0022). The volume of dentin removed was greater in TAC than with the CAC design. The amount of untouched canal wall area was significantly higher in conservative access design than with the traditional access design group.
近来,有几种通路洞的设计理念是尽量少去除牙体组织,从而提高根管治疗后牙齿的抗折能力。 为了研究在上颌磨牙根管器械治疗过程中,保守设计通路洞的效果。 将 80 颗无龋的上颌磨牙分为传统和保守通路组(各 40 颗)。在完成指定的通路准备后,将牙齿浸入卢戈尔斯溶液中对牙髓组织进行染色。使用 TruNatomy 锉系统进行根管器械操作。使用 CT-An 软件拍摄并重建器械前和器械后的纳米计算机断层扫描(CT)图像。根管体积分析使用 CT-Vol 软件完成。数据分析采用 Shapiro-Wilk 检验和独立 t 检验。 根据独立 t 检验,传统和保守通路设计组在器械操作前后的牙髓腔体积有明显变化。相比之下,TAC 组去除的平均牙本质体积要大得多(P = 0.0016)。独立 t 检验显示,传统根管治疗通路洞(TAC)和保守治疗通路洞(CAC)在未修整的管壁百分比上存在显著差异。与 CAC 组相比,TAC 组未修补区域的平均百分比明显较低(P = 0.0022)。 TAC 设计去除的牙本质量大于 CAC 设计。保守通路设计组的未修整管壁面积明显高于传统通路设计组。
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引用次数: 0
Evaluation of cytotoxicity and adaptability of a novel bioceramic root canal sealer: An in vitro and scanning electron microscope study 新型生物陶瓷根管封闭剂的细胞毒性和适应性评估:体外和扫描电子显微镜研究
Pub Date : 2024-03-01 DOI: 10.4103/jcde.jcde_40_24
Soha Alaa Emam, A. Mahran, M. Elshafei
Cytotoxicity and adaptability are among the highly imperative tests that should be performed on a novel endodontic material to ensure its successful implementation in endodontic treatment. Assess a recently introduced bioceramic root canal sealer CeramoSeal with TotalFill BC and AH plus sealers regarding the cytotoxicity and adaptability. Five sealer discs were prepared for each sealer and their extracts were cultured in 96-well plates containing human fibroblasts for 24 h. After their incubation, MTT solution was added to each well plate using an enzyme-linked immunosorbent assay plate reader was implemented to calculate the percentage of viable cells. Thirty mandibular single-rooted premolars were prepared using the Edge Endo rotary system, teeth were divided into three groups (n = 10) based on the sealer type: Group 1 CeramoSeal, Group 2 Totalfill, and Group 3 AH plus sealer. Teeth were sectioned longitudinally and viewed under a scanning electron microscope where the region with the gaps was identified and quantified as a percentage of the root canal’s overall area. One-way ANOVA test was used for cytotoxicity, while Kruskal–Wallis and Friedman’s tests were used for adaptability. Ceramoseal statistically significantly showed the lowest viability, at high concentrations AH plus showed the highest cell viability, while at lower concentration Totalfill BC sealer showed the highest cell viability percentage. The gap percentages were statistically significantly higher in Ceramoseal group, there was no statistically significant difference between AH Plus and Totalfill groups. Ceramoseal sealer exhibited the lowest viability and highest gap percentage compared to the other sealers.
为确保新型根管治疗材料在根管治疗中的成功应用,必须对其进行细胞毒性和适应性测试。 对最近推出的生物陶瓷根管封闭剂 CeramoSeal 与 TotalFill BC 和 AH plus 封闭剂的细胞毒性和适应性进行评估。 每种封闭剂都制备了五个封闭剂圆盘,并将其提取物在含有人成纤维细胞的 96 孔板中培养 24 小时。使用 Edge Endo 旋转系统制备了 30 颗下颌单根前臼齿,根据封闭剂类型将牙齿分为三组(n = 10):第 1 组为 CeramoSeal,第 2 组为 Totalfill,第 3 组为 AH 加封闭剂。对牙齿进行纵向切片,并在扫描电子显微镜下观察,确定有缝隙的区域,并以占根管总面积的百分比进行量化。 细胞毒性采用单因子方差分析,适应性采用 Kruskal-Wallis 和 Friedman 检验。 据统计,Ceramoseal 的细胞存活率最低,高浓度 AH plus 的细胞存活率最高,而低浓度 Totalfill BC 封闭剂的细胞存活率最高。据统计,Ceramoseal 组的间隙百分比明显更高,而 AH Plus 和 Totalfill 组之间没有明显的统计学差异。 与其他密封剂相比,Ceramoseal 密封剂的存活率最低,间隙百分比最高。
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引用次数: 0
In vitro evaluation of the effect of addition of biomaterials to carbamide peroxide on the bleaching efficacy and microhardness of enamel 在过氧化卡酰胺中添加生物材料对珐琅质漂白效果和微硬度影响的体外评估
Pub Date : 2024-03-01 DOI: 10.4103/jcde.jcde_310_23
Sowmya Kavoor, M. Ranjini, Naval Abdul Aziz, H. K. Ashok, Roopa R. Nadig
Teeth bleaching, although considered safe and conservative, cause microscopic changes in the tooth structure. The aim of this study is to evaluate the bleaching efficacy of carbamide peroxide (CP) bleaching gel when modified with the incorporation of bioactive glass (BG) and hydroxyapatite (HA) and its effect on enamel microhardness. Forty-five maxillary incisors were decoronated, artificially stained and mounted in acrylic. The samples were divided into three groups of 15 each and subjected to the following bleaching protocol for 8 h/day at 37°C for 2 weeks: Group 1 – 16% CP, Group 2 – CP modified with BG, and Group 3 – CP modified with hydroxyapatite (HA). Spectrophotometric color assessment using CIE L*a*b* system and Vickers microhardness were assessed before and after bleaching. Data were analyzed using Student’s paired t-test and one-way ANOVA followed by Tukey’s post hoc analysis. There was a significant change in color (L*a*b*) in all the three groups when compared to the baseline values. However, no significant difference in the total color change (ΔE) was observed between the three groups. Enamel microhardness reduced significantly in the CP group, whereas it increased in the BG and HA group after bleaching. Scanning electron microscopy images of BG and HA groups showed crystalline deposits suggesting mineral deposition. Addition of biomaterials can be a beneficial alternative to bleaching with CP alone, considering the increase in microhardness without hindering the bleaching action.
牙齿漂白虽然被认为是安全和保守的,但会引起牙齿结构的微观变化。本研究旨在评估过氧化卡巴酰胺(CP)漂白凝胶在加入生物活性玻璃(BG)和羟基磷灰石(HA)后的漂白效果及其对珐琅质微硬度的影响。 对 45 颗上颌门牙进行装饰、人工染色和丙烯酸镶嵌。样品被分为三组,每组 15 个,在 37°C 下每天漂白 8 小时,持续 2 周:第 1 组--16%氯化石蜡,第 2 组--用 BG 修饰的氯化石蜡,第 3 组--用羟基磷灰石(HA)修饰的氯化石蜡。漂白前后使用 CIE L*a*b* 系统进行分光光度颜色评估,并评估维氏硬度。数据分析采用学生配对 t 检验和单因素方差分析,然后进行 Tukey 事后分析。 与基线值相比,三组的色泽(L*a*b*)都有明显变化。但是,三组之间的总颜色变化(ΔE)没有明显差异。漂白后,CP 组的釉质微硬度明显降低,而 BG 和 HA 组的釉质微硬度则有所提高。BG 组和 HA 组的扫描电子显微镜图像显示有结晶沉积,表明有矿物质沉积。 考虑到在不妨碍漂白作用的情况下增加微硬度,添加生物材料可能是单独使用氯化石蜡漂白的一种有益替代方法。
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引用次数: 0
Comparative evaluation of incidence of dentinal defects after root canal preparation using three different endodontic retreatment systems – An in vitro study 使用三种不同的牙髓再治疗系统进行根管预备后牙本质缺损发生率的比较评估 - 一项体外研究
Pub Date : 2024-03-01 DOI: 10.4103/jcde.jcde_266_23
S. Aarthi, J. S. Sivakumar, A. Sivakumar, J. S. Soundappan, M. Chittrarasu, G. Jayanthi
Safe and efficient removal of all root filling materials from the root canal system without compromising radicular dentin structure is essential for optimal nonsurgical retreatment. The aim of this study was to evaluate and compare the incidence of dentinal defects caused during root canal filling removal using conventional, rotary, and reciprocating retreatment file systems. A detailed protocol explaining purpose and procedures of the study was submitted to the Institutional Ethics Committee and ethical clearance obtained. Sixty human maxillary permanent central incisors were collected and decoronated to 12-mm standardized length. The canals prepared up to a master apical file size F3 with Protaper hand files, obturated using AH plus sealer, examined under the stereomicroscope (×40 magnification): Group I: Control (n = 15), Group II: Conventional (n = 15), Group III: Protaper Universal Retreatment Files (n = 15), and Group IV: Reciproc Blue (n = 15). After instrumentation, teeth were sectioned at 3, 6, and 9 mm from the apex to evaluate the presence of dentinal defects under the stereomicroscope. Statistics were performed using the SPSS, version, 25 (SPSS Inc., Chicago, IL, USA). Initially, normality test was done using the Shapiro–Wilk test and data were not normally distributed followed by Kruskal–Wallis test. P < 0.05 is considered statistically significant. Maximum percentage increase in dentinal defects was observed in Protaper Universal Retreatment Files followed by Conventional method and Reciproc Blue. Significantly Reciproc Blue reduced the incidence of dentinal defects after root canal preparation.
在不影响根管牙本质结构的前提下,安全有效地去除根管系统中的所有根管充填材料对于实现最佳的非手术再治疗至关重要。 本研究旨在评估和比较使用传统、旋转和往复式再治疗锉系统去除根管充填物时造成的牙本质缺损的发生率。 该研究向机构伦理委员会提交了一份详细的方案,解释了研究的目的和程序,并获得了伦理许可。 收集了 60 颗人类上颌恒中切牙,并装饰成 12 毫米的标准长度。使用Protaper手用锉制备根尖主锉大小为F3的根管,使用AH加封闭剂封闭,在体视显微镜(×40倍)下检查:第一组:对照组(n = 15),第二组:常规组(n = 15),第三组:Protaper 通用修复锉(n = 15),第四组:Reciproc Blue(n = 15)。使用器械后,在距离牙尖 3、6 和 9 毫米处对牙齿进行切片,以便在体视显微镜下评估牙本质缺损的存在。 使用 SPSS 25 版本(SPSS Inc.)首先使用 Shapiro-Wilk 检验进行正态性检验,如果数据不符合正态分布,则进行 Kruskal-Wallis 检验。P < 0.05 被认为具有统计学意义。 Protaper通用修整锉的牙本质缺损增加百分比最大,其次是传统方法和Reciproc Blue。 Reciproc Blue 能显著降低根管预备后牙本质缺损的发生率。
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引用次数: 0
Nanorevolution: Trends and future perspectives 纳米革命:趋势与未来展望
Pub Date : 2024-03-01 DOI: 10.4103/jcde.jcde_71_24
Shishir Singh, S. Saha
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引用次数: 0
Evaluation of minimum inhibitory concentration and minimum bactericidal concentration of royal jelly against Enterococcus faecalis, Staphylococcus aureus, and Candida albicans 评估蜂王浆对粪肠球菌、金黄色葡萄球菌和白色念珠菌的最低抑菌浓度和最低杀菌浓度
Pub Date : 2024-03-01 DOI: 10.4103/jcde.jcde_234_23
Vivek Devidas Mahale, Sonali Sharma
The objective of this study was to ascertain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of royal jelly (RJ) against three microorganisms frequently linked with endodontic infections: Staphylococcus aureus, Enterococcus faecalis, and Candida albicans. Freshly harvested RJ was prepared at different concentrations (20%, 10%, 5%, 2.5%, and 1.25%) in distilled water. The microbial cultures of the target organisms were prepared. MIC was determined using a broth dilution technique, monitoring microbial growth. MBC was determined by inoculating agar plates with samples from tubes showing no apparent growth and evaluating the presence of bacterial or fungal growth following the incubation period. For S. aureus, the MIC and MBC were 5 mg/ml of RJ. For E. faecalis, the MIC and MBC were 10 mg/ml of RJ. For C. albicans, both MIC and MBC were 10 mg/ml of RJ. The findings demonstrated RJ’s potential to inhibit and eliminate these pathogenic microorganisms, making it a potential candidate for endodontic infection control. The antimicrobial properties of RJ against S. aureus, E. faecalis, and C. albicans present a promising avenue for enhancing infection control in endodontics. Additional investigations are needed to refine its use in clinical settings, especially in cases with mixed microbial infections.
这项研究的目的是确定蜂王浆(RJ)对三种经常与牙髓感染有关的微生物的最低抑菌浓度(MIC)和最低杀菌浓度(MBC):金黄色葡萄球菌、粪肠球菌和白色念珠菌。 在蒸馏水中配制不同浓度(20%、10%、5%、2.5% 和 1.25%)的新鲜采摘的 RJ。制备目标生物的微生物培养物。使用肉汤稀释技术测定 MIC,监测微生物生长。在琼脂平板上接种无明显生长的试管样品,并在培养期后评估是否有细菌或真菌生长,从而确定 MBC。 金黄色葡萄球菌的 MIC 和 MBC 均为 5 毫克/毫升 RJ。对于粪肠球菌,RJ 的 MIC 和 MBC 均为 10 毫克/毫升。对于白僵菌,RJ 的 MIC 和 MBC 均为 10 毫克/毫升。研究结果表明,RJ 具有抑制和消除这些病原微生物的潜力,使其成为牙髓感染控制的潜在候选药物。 RJ 对金黄色葡萄球菌、粪大肠杆菌和白念珠菌的抗菌特性为加强牙髓病的感染控制提供了一个前景广阔的途径。还需要进行更多的研究来完善其在临床环境中的应用,尤其是在混合微生物感染的病例中。
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引用次数: 0
Root canal dentin wear during final irrigation in endodontic retreatment with passive ultrasonic irrigation and easy clean instruments 在使用被动超声波灌洗和易清洁器械进行牙髓再治疗的最终灌洗过程中根管牙本质的磨损情况
Pub Date : 2024-03-01 DOI: 10.4103/jcde.jcde_137_23
L. S. Alves, F. Ormiga, C. D. de Lima, R. Lopes, H. Gusman
Complementary procedures have been proposed to improve the endodontic retreatments. The aim of this study was to evaluate, by microcomputed tomography (micro-CT), the dentin wear caused by passive ultrasonic irrigation (PUI) and easy clean (EC) instruments, when used for the final removal of filling material during endodontic retreatment. Thirty-six mesial roots of the lower first molars were divided into four groups (n = 9), according to the final irrigation and sealer: PUI/AH Plus, EC/AH Plus, PUI/Total Fill (TF), and EC/TF. Canal volume was evaluated, both before and after the final irrigation, by micro-CT analysis. The Kruskal–Wallis test was used to compare the volumes among groups and the paired Wilcoxon test was used to compare the volume values before and after the final irrigation within each group. Both complementary cleaning procedures promoted dentin wear that was observed only when the volume was analyzed for each third of the root canal. In the apical third, both instruments showed significant wear, where the use of the EC instrument resulted in greater wear than the PUI, in teeth filled with both AH Plus and TF BC Sealers (P < 0.05). Both complementary cleaning procedures promoted dentin wear and must be used cautiously.
有人建议采用互补程序来改进牙髓退修治疗。 本研究的目的是通过显微计算机断层扫描(micro-CT)评估牙髓再治疗过程中被动超声冲洗(PUI)和易清洁(EC)器械用于最终去除充填材料时造成的牙本质磨损。 根据最终灌洗和封闭剂的不同,下第一磨牙的 36 个中根被分为四组(n = 9):PUI/AH Plus、EC/AH Plus、PUI/Total Fill (TF) 和 EC/TF。在最终灌洗前后,均通过显微 CT 分析评估了牙槽体积。Kruskal-Wallis 检验用于比较各组间的体积,配对 Wilcoxon 检验用于比较各组内最终冲洗前后的体积值。 两种辅助清洁程序都会促进牙本质磨损,只有在分析根管三分之一处的体积时才能观察到牙本质磨损。在使用 AH Plus 和 TF BC 密封剂填充的牙齿中,两种器械在根尖三分之一处都显示出明显的磨损,其中使用 EC 器械比使用 PUI 造成的磨损更大(P < 0.05)。 两种辅助清洁程序都会促进牙本质磨损,必须谨慎使用。
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引用次数: 0
Is a mix – A fix? “A microscopic analysis of depth of penetration of three combinations of irrigants” 混合剂是一种固定剂吗?"三种灌溉剂组合渗透深度的显微分析"
Pub Date : 2024-02-01 DOI: 10.4103/jcde.jcde_265_23
Yantrapragada Lakshmi Sunanda, K. Parvathaneni, T. Raju, Abitha Seshadri, Nadimpalli Mahendra Varma, G. Dondapati
The aim of the study is to evaluate the penetration depth of irrigants mixed with NaOCl into dentinal tubules at the apical third of the root canal using a confocal laser scanning microscope (CLSM). Thirty-six single-rooted teeth were selected, decoronated, and straight-line access established. Then, the canal was enlarged with hand Protaper till size F3. The samples were divided randomly into three groups: Group 1 was irrigated with 3% NaOCl mixed with 1% phytic acid; Group 2 was irrigated with 3% NaOCl mixed with 18% etidronic acid; and Group 3 was irrigated with 3% NaOCl mixed with chitosan. The samples were subjected to CLSM evaluation. One-way analysis of variance with Tukey’s post hoc was used for statistical analysis. Group 3 (NaOCl with chitosan) showed a greater depth of penetration in comparison to Groups 1 and 2. Within limitations, it can be concluded that chitosan, in combination with NaOCl can be used as an alternative to the current irrigation protocol.
本研究的目的是使用激光共聚焦扫描显微镜(CLSM)评估与 NaOCl 混合的冲洗剂在根管顶端三分之一处牙本质小管中的渗透深度。 我们选择了 36 颗单根牙齿,对其进行了装饰,并建立了直线通路。然后,用手工 Protaper 扩大根管至 F3 大小。样本被随机分为三组:第一组用 3% NaOCl 混合 1% 植酸进行灌洗;第二组用 3% NaOCl 混合 18% 乙膦酸进行灌洗;第三组用 3% NaOCl 混合壳聚糖进行灌洗。对样品进行了 CLSM 评估。统计分析采用单因素方差分析和 Tukey 后检验。 与第 1 组和第 2 组相比,第 3 组(NaOCl 与壳聚糖)的渗透深度更大。 在有限的条件下,可以得出这样的结论:壳聚糖与 NaOCl 结合使用可作为当前灌溉方案的替代品。
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Journal of Conservative Dentistry and Endodontics
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