S. Averyanov, D. Akhmetova, S. Lazarev, I. Iskhakov, Lyubov' Ryabyh, Nazir Alyev
The high prevalence of dental diseases among different contingents of young people (conscripts, students of colleges and universities, including students from abroad) has no tendency to decrease. Young people with high prevalence of dental anomalies tend to disorders of the functions of the dental system, aesthetic changes and the formation of a psychoemotional complex. The conducted research establishes the need for the treatment of dental anomalies in young people.
{"title":"THE PREVALENCE OF DENTAL ANOMALIES IN YOUNG POPULATIONS AND THE NEED FOR THEIR TREATMENT","authors":"S. Averyanov, D. Akhmetova, S. Lazarev, I. Iskhakov, Lyubov' Ryabyh, Nazir Alyev","doi":"10.35630/2023/13/3.313","DOIUrl":"https://doi.org/10.35630/2023/13/3.313","url":null,"abstract":"The high prevalence of dental diseases among different contingents of young people (conscripts, students of colleges and universities, including students from abroad) has no tendency to decrease. Young people with high prevalence of dental anomalies tend to disorders of the functions of the dental system, aesthetic changes and the formation of a psychoemotional complex. The conducted research establishes the need for the treatment of dental anomalies in young people.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42430832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Boiko, Yu. I. Dotsenko, R. Mukhamedzyanova, Tatiana Porotikova, Anzhela Ramaeva, Dinara Utesheva, R. Bisalieva
The role of infectious pathology in the genesis of infertility is still being studied. The present review considers possible mechanisms of infertility development associated with the microbial profile of the male reproductive system. It makes an attempt to study the role of pathogenic properties of microorganisms in the development of infertile conditions if an inflammatory process is localized in the male urogenital tract, in particular its effects on the fertility properties of spermatozoa (reduction in their number, inhibition of their motility, changes in their morphology and fertilizing capacity). Besides, the review presents a discussion on the role of reactivity and resistance of an organism in maintaining chronic inflammation in male reproductive organs.
{"title":"ROLE OF MICROBIAL FACTOR IN MALE INFERTILITY PATHOGENESIS. LITERATURE REVIEW","authors":"O. Boiko, Yu. I. Dotsenko, R. Mukhamedzyanova, Tatiana Porotikova, Anzhela Ramaeva, Dinara Utesheva, R. Bisalieva","doi":"10.35630/2023/13/3.314","DOIUrl":"https://doi.org/10.35630/2023/13/3.314","url":null,"abstract":"The role of infectious pathology in the genesis of infertility is still being studied. The present review considers possible mechanisms of infertility development associated with the microbial profile of the male reproductive system. It makes an attempt to study the role of pathogenic properties of microorganisms in the development of infertile conditions if an inflammatory process is localized in the male urogenital tract, in particular its effects on the fertility properties of spermatozoa (reduction in their number, inhibition of their motility, changes in their morphology and fertilizing capacity). Besides, the review presents a discussion on the role of reactivity and resistance of an organism in maintaining chronic inflammation in male reproductive organs.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45474877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: In recent decades, the incidence of melanoma among European population has been steadily increasing. In addition, the age of patients with this pathology is declining - today more than half of patients with melanoma are under 65 years. Melanoma is an aggressive metastatic tumor that is difficult to treat. The tumor is resistant to chemotherapy and radiotherapy. However, the mechanism of photodynamic action differs from the mechanism of cell damage by antitumor drugs or ionizing radiation. Therefore, there is a high probability that with the help of photodynamic therapy it will be possible to overcome the resistance of melanoma cells to damage. Materials and methods: The study was performed on melanoma tumors of B16 mice. The due methylene blue (MB) was used as a photosensitizer through the known affinity of this colorant to the melanin pigment of melanoma cells. The experiments used the radiation of two semiconductor lasers (Fotonika Plus, Cherkasy. Ukraine) with a wavelength of 660 nm, which corresponds to the maximum absorption of methylene blue. Results:The possibility of increasing the anti-melanoma photodynamic activity of MB with the help of a modifier - the natural polysaccharide chitosan was studied. B16 melanoma has been shown to be sensitive to PDT with MB and its sensitivity can be increased by using MB with the natural polysaccharide chitosan, which has immunoadjuvant properties. Conclusion: As a result of the presented research, a variant of PDT of B16 melanoma of mice was developed using MB sensitizer and chitosan immunoadjuvant, which includes intratumoral injection of MB together with chitosan, irradiation of tumors with a red laser with a wavelength of 660 nm, as well as the use of chitosan as an immunomodulatory factor.
{"title":"RED LASER PHOTODYNAMIC THERAPY OF MELANOMA B16 IN AN EXPERIMENTAL MODEL IN VIVO","authors":"I. Prokopenko, S. Konovalenko","doi":"10.35630/2023/13/3.304","DOIUrl":"https://doi.org/10.35630/2023/13/3.304","url":null,"abstract":"Introduction: In recent decades, the incidence of melanoma among European population has been steadily increasing. In addition, the age of patients with this pathology is declining - today more than half of patients with melanoma are under 65 years. Melanoma is an aggressive metastatic tumor that is difficult to treat. The tumor is resistant to chemotherapy and radiotherapy. However, the mechanism of photodynamic action differs from the mechanism of cell damage by antitumor drugs or ionizing radiation. Therefore, there is a high probability that with the help of photodynamic therapy it will be possible to overcome the resistance of melanoma cells to damage. Materials and methods: The study was performed on melanoma tumors of B16 mice. The due methylene blue (MB) was used as a photosensitizer through the known affinity of this colorant to the melanin pigment of melanoma cells. The experiments used the radiation of two semiconductor lasers (Fotonika Plus, Cherkasy. Ukraine) with a wavelength of 660 nm, which corresponds to the maximum absorption of methylene blue. Results:The possibility of increasing the anti-melanoma photodynamic activity of MB with the help of a modifier - the natural polysaccharide chitosan was studied. B16 melanoma has been shown to be sensitive to PDT with MB and its sensitivity can be increased by using MB with the natural polysaccharide chitosan, which has immunoadjuvant properties. Conclusion: As a result of the presented research, a variant of PDT of B16 melanoma of mice was developed using MB sensitizer and chitosan immunoadjuvant, which includes intratumoral injection of MB together with chitosan, irradiation of tumors with a red laser with a wavelength of 660 nm, as well as the use of chitosan as an immunomodulatory factor.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45125307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
DMITRY PARSHIN, A. Topchiev, A. Protasov, M. Topchiev, A. Fedoseev, Lev Brusnev, Vladislav Murzov, M.K. Misrikhanov
This research involves a comparative study of the immunohistochemical features of the inflammatory response of the anterior abdominal wall tissues after implantation of the autoderm and polypropylene mesh in rats. The object of the study were 36 Wistar rats, which were divided into two groups of 18 specimens each. In the first group, a polypropylene mesh was implanted; in the second group, de-epithelialized skin (autoderm) was implanted. On days 3, 7, 14, 30, and 90, we studied: CD68 as a pan-macrophage marker, CD3 as a pan-lymphocyte marker, and Bcl-2 the apoptosis regulator. Statistical analysis was performed using the Spearman-Rho-Test. The peak of an acute inflammatory response in the tissues after the implantation of polypropylene mesh and autoderm occurs 72 hours after the surgery. Arresting of acute inflammatory response after autodermal implantation is achieved 30 days after surgery. A specific reaction of the anterior abdominal wall tissues to polypropylene, in contrast to the autoderm, is the formation of a persistent chronic inflammatory response 90 days after the operation, which is due to the starting biodegradation of the synthetic implant. The Spearman-Rho-Test showed a highly significant correlation between CD68 and CD3 (r = 0.341, p = 0.001). A high correlation was found between CD68 and Bcl-2 (r = 0.195, p = 0.033) and CD3 and Bcl-2 (r = 0.220, p = 0.021). CD3, CD68 and Bcl-2 markers give evidence of the severity of the inflammatory response.
{"title":"IMMUNOHISTOCHEMICAL TRENDS OF THE CONSEQUENCES OF MESH AND AUTODERM IMPLANTATION INTO THE ANTERIOR ABDOMINAL WALL IN RATS","authors":"DMITRY PARSHIN, A. Topchiev, A. Protasov, M. Topchiev, A. Fedoseev, Lev Brusnev, Vladislav Murzov, M.K. Misrikhanov","doi":"10.35630/2023/13/3.312","DOIUrl":"https://doi.org/10.35630/2023/13/3.312","url":null,"abstract":"This research involves a comparative study of the immunohistochemical features of the inflammatory response of the anterior abdominal wall tissues after implantation of the autoderm and polypropylene mesh in rats. The object of the study were 36 Wistar rats, which were divided into two groups of 18 specimens each. In the first group, a polypropylene mesh was implanted; in the second group, de-epithelialized skin (autoderm) was implanted. On days 3, 7, 14, 30, and 90, we studied: CD68 as a pan-macrophage marker, CD3 as a pan-lymphocyte marker, and Bcl-2 the apoptosis regulator. Statistical analysis was performed using the Spearman-Rho-Test. The peak of an acute inflammatory response in the tissues after the implantation of polypropylene mesh and autoderm occurs 72 hours after the surgery. Arresting of acute inflammatory response after autodermal implantation is achieved 30 days after surgery. A specific reaction of the anterior abdominal wall tissues to polypropylene, in contrast to the autoderm, is the formation of a persistent chronic inflammatory response 90 days after the operation, which is due to the starting biodegradation of the synthetic implant. The Spearman-Rho-Test showed a highly significant correlation between CD68 and CD3 (r = 0.341, p = 0.001). A high correlation was found between CD68 and Bcl-2 (r = 0.195, p = 0.033) and CD3 and Bcl-2 (r = 0.220, p = 0.021). CD3, CD68 and Bcl-2 markers give evidence of the severity of the inflammatory response.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45236057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natalia Khalidolla, P. Akhmedova, M. Shapovalova, I.N. Moroz, A. Abdullaeva
Relevance: The WHO Bulletin (2000) indicated that between one and five people in the working population have some form of mental health problem at any given time (1). This figure is on the rise and has been proven to affect their employability, work efficiency and quality of life. These problems are also common among healthcare workers, with more than 20% of healthcare workers worldwide experiencing mental health problems (2-4). Irrational and rational cognitions/beliefs are evaluative cognitive attitudes (structures) consistently associated with distress and psychopathology; at the same time, rational thinking is a determining factor for emotional stability. Goals of the study: 1. To conduct a comparative analysis of irrational attitudes in medical residents as a factor of professional maladaptation at the stage of clinical residency. 2. To formulate recommendations for its timely correction. Research Methodology: The methodology for diagnosing irrational attitudes (Survey of Personal Beliefs, SPB) was developed on the basis of the theory of rational-emotive therapy (RET) by Albert Ellis. The following authors’ methods were used: 1) Diagnosis of irrational attitudes - the Survey of Personal Beliefs (SPB) by H. Kassinove; 2) Questionnaire of socio-psychological adaptation, SPA (Test of Personal Adjustment) by K. Rogers, R. Diamond. Results: It was revealed that the majority of medical residents had a high value of irrational attitudes on the scales "Obligation towards oneself" and "Catastrophization". The results of the empirical study revealed significant differences between the two groups of residents using the Statistics 21.0 application package and the Mann-Whitney univariate statistical test. Conclusions and Recommendations: Residents at the stage of training in clinical residency use two irrational (erroneous) attitudes as Obligation to oneself and Catastrophization, which interfere with adequate cognitive processing, and also serve as an additional source of stress for medical workers. The schemes of psychological support of the residents were developed in order to identify irrational beliefs and timely correct them using psychological techniques (cognitive-behavioral therapy techniques, rational-emotive therapy) at the stage of training in clinical residency.
{"title":"IRRATIONAL BELIEFS AS A FACTOR OF PROFESSIONAL DISADAPTATION OF MEDICAL WORKERS","authors":"Natalia Khalidolla, P. Akhmedova, M. Shapovalova, I.N. Moroz, A. Abdullaeva","doi":"10.35630/2023/13/3.303","DOIUrl":"https://doi.org/10.35630/2023/13/3.303","url":null,"abstract":"Relevance: The WHO Bulletin (2000) indicated that between one and five people in the working population have some form of mental health problem at any given time (1). This figure is on the rise and has been proven to affect their employability, work efficiency and quality of life. These problems are also common among healthcare workers, with more than 20% of healthcare workers worldwide experiencing mental health problems (2-4). Irrational and rational cognitions/beliefs are evaluative cognitive attitudes (structures) consistently associated with distress and psychopathology; at the same time, rational thinking is a determining factor for emotional stability. Goals of the study: 1. To conduct a comparative analysis of irrational attitudes in medical residents as a factor of professional maladaptation at the stage of clinical residency. 2. To formulate recommendations for its timely correction. Research Methodology: The methodology for diagnosing irrational attitudes (Survey of Personal Beliefs, SPB) was developed on the basis of the theory of rational-emotive therapy (RET) by Albert Ellis. The following authors’ methods were used: 1) Diagnosis of irrational attitudes - the Survey of Personal Beliefs (SPB) by H. Kassinove; 2) Questionnaire of socio-psychological adaptation, SPA (Test of Personal Adjustment) by K. Rogers, R. Diamond. Results: It was revealed that the majority of medical residents had a high value of irrational attitudes on the scales \"Obligation towards oneself\" and \"Catastrophization\". The results of the empirical study revealed significant differences between the two groups of residents using the Statistics 21.0 application package and the Mann-Whitney univariate statistical test. Conclusions and Recommendations: Residents at the stage of training in clinical residency use two irrational (erroneous) attitudes as Obligation to oneself and Catastrophization, which interfere with adequate cognitive processing, and also serve as an additional source of stress for medical workers. The schemes of psychological support of the residents were developed in order to identify irrational beliefs and timely correct them using psychological techniques (cognitive-behavioral therapy techniques, rational-emotive therapy) at the stage of training in clinical residency.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44119952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adam Sheikh Said, D. Kicha, Mayeh Omar, Y. Gushchina, Amenti Teka Geferso, J. Handuleh
Background: Infection prevention is essential for providing safe and quality facility-level services. Worldwide, morbidity and mortality are primarily due to preventable infectious diseases which account for 62% of all deaths in Africa and 31% in Southeast Asia. Therefore, infection prevention and control measures enhance the protection of vulnerable people. Objective: The objective of this study is to determine infection prevention practice and associated factors among healthcare workers working in government health facilities of Mogadishu, Somalia, 2022 Methods: A quantitative cross-sectional study was conducted from March 01, 2022, to May 30, 2022 on 562 healthcare workers of public health facilities in Mogadishu town. After obtaining consent from the study participants, data was collected using pretested, self-administered, and standardized questionnaires adapted from other studies. After the data was collected, it was processed, cleaned, and analyzed using SPSS version 26. A logistic regression model was computed to measure the association between the predictor and outcome variables. A P-value of.05 with a 95% CI was used as the cut-off point to declare the level of statistical significance. Results: The study found that 236 (42.4%) (95%CI: 1.38-1.47) of the 556 respondents had a good practice of infection prevention. In the multivariate logistic regression analysis, attitudes of health care workers toward infection prevention (AOR=0.478, 95 percent CI: 0.316, 0.723), occupational training (AOR:0.177, 95%CI:0.177,0.591), work experience of health care workers (AOR:3.215,95%CI:1.712,6.038), availability of infection prevention guidelines (AOR: 0.489,95%CI:0.284,0.842), budget availability for infection prevention (AOR: 0.421, 95%CI: 0.245,0.723) were among factors significantly associated with infection prevention practice. Conclusion: The magnitude of infection prevention practice was low (42.4%) compared to other studies. The results of the study showed that attitude, knowledge work experience, training, needlestick injury, vaccination against HepB and other infectious diseases, availability of infection prevention guidelines, availability of hand rub in the room, and availability of budget for infection prevention were among factors associated with infection prevention practice.
{"title":"INFECTION PREVENTION PRACTICE AND ASSOCIATED FACTORS AMONG HEALTH CARE WORKERS IN PUBLIC HEALTH FACILITIES OF MOGADISHU, SOMALIA, 2022","authors":"Adam Sheikh Said, D. Kicha, Mayeh Omar, Y. Gushchina, Amenti Teka Geferso, J. Handuleh","doi":"10.35630/2023/13/3.301","DOIUrl":"https://doi.org/10.35630/2023/13/3.301","url":null,"abstract":"Background: Infection prevention is essential for providing safe and quality facility-level services. Worldwide, morbidity and mortality are primarily due to preventable infectious diseases which account for 62% of all deaths in Africa and 31% in Southeast Asia. Therefore, infection prevention and control measures enhance the protection of vulnerable people. Objective: The objective of this study is to determine infection prevention practice and associated factors among healthcare workers working in government health facilities of Mogadishu, Somalia, 2022 Methods: A quantitative cross-sectional study was conducted from March 01, 2022, to May 30, 2022 on 562 healthcare workers of public health facilities in Mogadishu town. After obtaining consent from the study participants, data was collected using pretested, self-administered, and standardized questionnaires adapted from other studies. After the data was collected, it was processed, cleaned, and analyzed using SPSS version 26. A logistic regression model was computed to measure the association between the predictor and outcome variables. A P-value of.05 with a 95% CI was used as the cut-off point to declare the level of statistical significance. Results: The study found that 236 (42.4%) (95%CI: 1.38-1.47) of the 556 respondents had a good practice of infection prevention. In the multivariate logistic regression analysis, attitudes of health care workers toward infection prevention (AOR=0.478, 95 percent CI: 0.316, 0.723), occupational training (AOR:0.177, 95%CI:0.177,0.591), work experience of health care workers (AOR:3.215,95%CI:1.712,6.038), availability of infection prevention guidelines (AOR: 0.489,95%CI:0.284,0.842), budget availability for infection prevention (AOR: 0.421, 95%CI: 0.245,0.723) were among factors significantly associated with infection prevention practice. Conclusion: The magnitude of infection prevention practice was low (42.4%) compared to other studies. The results of the study showed that attitude, knowledge work experience, training, needlestick injury, vaccination against HepB and other infectious diseases, availability of infection prevention guidelines, availability of hand rub in the room, and availability of budget for infection prevention were among factors associated with infection prevention practice.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42376441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kalashnikov, Yu.N. Litun, Yuriy Stavinskyi, V. Protsenko, Y. Solonitsyn
Background: Some biochemical indicators in patients with disorders of reparative osteogenesis were described in a sufficient number of studies, but the role of factors affecting the metabolism of bone tissue and the ways of their correction are not sufficiently studied .. For effective prediction of the course of reparative osteogenesis in fractures, it is important to identify early markers of impaired consolidation and, accordingly, its pathogenetic correction. Purpose: to determine biochemical indicators of the course of reparative osteogenesis in patients with multiple and monolocal fractures of the bones of the lower limb. Materials and Methods: 44 patients aged 23 to 47 years were examined. To assess the ratio and intensity of the processes of biosynthesis and breakdown of collagen, as the major structural protein of connective tissue, the following indicators were determined in the blood serum of patients: fractions of hydroxyproline, oxyproline, collagenase, cathepsin B, elastase, proteolysis-antielastase inhibitors. In addition, indicators of mineral metabolism were also determined in the blood serum of patients: the content of calcium, phosphorus, alkaline phosphatase activity. Results: A comparative analysis and comparison of the results of the biochemical examination of patients with closed tibial fractures and multiple fractures revealed that already at the beginning of the research, deviations from the norm in the indicators of both mineral and collagen metabolism were observed. Both groups of the patients are characterized by the presence of disturbances in the ratio between calcium and phosphorus, and in patients with multiple fractures - an increase in the level of alkaline phosphatase. The detected changes in the mineral metabolism in patients with multiple fractures had a more pronounced and intense character due to the multiple injuries and presence of a large number of destroyed cells in the first days after the injury. Conclusions: Indicators of the unfavorable course of reparative osteogenesis in patients with bone fractures are a decrease in the content of calcium/phosphorus in blood serum, an increase in the level of free oxyproline and collagenolytic enzymes (collagenase, elastase, cathepsin B). Besides, there is a decrease in the level of proteolysis inhibitors (antielastase, 2-macroglobulin) and a decrease in suspension stability and antioxidant activity, increase in blood fibrinolytic activity. Our findings justify the need for prophylactic drugs that improve reparative osteogenesis soon after the injury (within a month) in patients with multiple fractures in order to activate the processes of reparative regeneration.
{"title":"BIOCHEMICAL SIGNS OF THE COURSE OF REPARATIVE OSTEOGENESIS IN PATIENTS WITH FRACTURES OF THE LOWER EXTREMITY","authors":"A. Kalashnikov, Yu.N. Litun, Yuriy Stavinskyi, V. Protsenko, Y. Solonitsyn","doi":"10.35630/2023/13/3.311","DOIUrl":"https://doi.org/10.35630/2023/13/3.311","url":null,"abstract":"Background: Some biochemical indicators in patients with disorders of reparative osteogenesis were described in a sufficient number of studies, but the role of factors affecting the metabolism of bone tissue and the ways of their correction are not sufficiently studied .. For effective prediction of the course of reparative osteogenesis in fractures, it is important to identify early markers of impaired consolidation and, accordingly, its pathogenetic correction. Purpose: to determine biochemical indicators of the course of reparative osteogenesis in patients with multiple and monolocal fractures of the bones of the lower limb. Materials and Methods: 44 patients aged 23 to 47 years were examined. To assess the ratio and intensity of the processes of biosynthesis and breakdown of collagen, as the major structural protein of connective tissue, the following indicators were determined in the blood serum of patients: fractions of hydroxyproline, oxyproline, collagenase, cathepsin B, elastase, proteolysis-antielastase inhibitors. In addition, indicators of mineral metabolism were also determined in the blood serum of patients: the content of calcium, phosphorus, alkaline phosphatase activity. Results: A comparative analysis and comparison of the results of the biochemical examination of patients with closed tibial fractures and multiple fractures revealed that already at the beginning of the research, deviations from the norm in the indicators of both mineral and collagen metabolism were observed. Both groups of the patients are characterized by the presence of disturbances in the ratio between calcium and phosphorus, and in patients with multiple fractures - an increase in the level of alkaline phosphatase. The detected changes in the mineral metabolism in patients with multiple fractures had a more pronounced and intense character due to the multiple injuries and presence of a large number of destroyed cells in the first days after the injury. Conclusions: Indicators of the unfavorable course of reparative osteogenesis in patients with bone fractures are a decrease in the content of calcium/phosphorus in blood serum, an increase in the level of free oxyproline and collagenolytic enzymes (collagenase, elastase, cathepsin B). Besides, there is a decrease in the level of proteolysis inhibitors (antielastase, 2-macroglobulin) and a decrease in suspension stability and antioxidant activity, increase in blood fibrinolytic activity. Our findings justify the need for prophylactic drugs that improve reparative osteogenesis soon after the injury (within a month) in patients with multiple fractures in order to activate the processes of reparative regeneration.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46943147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In experiments on Wistar rats, it was shown that the prophylactic administration of melatonin at a dose of 5 mg/kg helps to reduce the manifestations of toxic coagulopathy, preventing the development of micro- and macrohemodynamic disorders in rats with chronic cadmium intoxication. Correlations between changes in indicators of the hemostasis system with the restoration of linear and volumetric blood flow rates and a decrease in the Pourcelot peripheral resistive index were revealed. These effects correlated with a decrease in the level of lipid peroxidation products and the activity of antioxidant enzymes in the blood under the influence of melatonin. The results of the experiments suggest further studies on using melatonin for the prevention and correction of the procoagulative and pathocirculatory effects of cadmium.
{"title":"RELATIONSHIP BETWEEN HAEMOSTATIC AND MACRO-MICROHEMODYNAMIC PARAMETERS IN RATS UNDER EXPERIMENTAL EXPOSURE TO CADMIUM. MEANS OF PREVENTION","authors":"V. Brin, E. Gagloeva, O. Kabisov","doi":"10.35630/2023/13/3.307","DOIUrl":"https://doi.org/10.35630/2023/13/3.307","url":null,"abstract":"In experiments on Wistar rats, it was shown that the prophylactic administration of melatonin at a dose of 5 mg/kg helps to reduce the manifestations of toxic coagulopathy, preventing the development of micro- and macrohemodynamic disorders in rats with chronic cadmium intoxication. Correlations between changes in indicators of the hemostasis system with the restoration of linear and volumetric blood flow rates and a decrease in the Pourcelot peripheral resistive index were revealed. These effects correlated with a decrease in the level of lipid peroxidation products and the activity of antioxidant enzymes in the blood under the influence of melatonin. The results of the experiments suggest further studies on using melatonin for the prevention and correction of the procoagulative and pathocirculatory effects of cadmium.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43729538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Domenyuk, O. Sumkina, Natalya Mikutskaya, T. Kochkonyan, A. Markovsky, Dmitry Matsukatov, Yuri Harutyunyan, O. Ivanyuta, S. Domenyuk
The expansion of the most relevant priority fields in fundamental and applied medicine, along with the progress in modern dentistry and reconstructive maxillofacial surgery, requires in-depth understanding of the morphological and functional status of the maxillary alveolar process bone tissue and knowledge of the mandibular alveolar part, thus allowing selecting a reasonable treatment tactics for maxillofacial pathologies. Qualitative features of maxillary alveolar process microarchitectonics, angioarchitectonics, as well as the periodontal ligament arrangement at various levels of the teeth roots, histological and morphometric studies of bone tissue in the frontal and distal sections, as well as the maxillary segments of the maxillary medial incisors and the first molars were studied in 5 certified male cadavers with preserved dentition. The histological findings show, that the microarchitectonics of the maxillary alveolar process features a lamellar bone consists of plates that are adjacent tightly to each other. The bone plates orientation in the frontal section is longitudinal, while in the distal part it is along concentric circles located around the Haversian canals. The angioarchitectonics of the maxillary alveolar process features tubular structures, which run mainly perpendicular to the bone surface, with numerous anastomoses. As the morphometric analysis of the maxillary alveolar process vascular system shows, in the frontal section the number of vessels per 1 mm2 is 22.41± 1.76 - 22.87± 2.08; in the distal section − 23.94± 1.88 - 25.02±2.69 (p≤0.01); the average diameter of vessels in the frontal section is 25.34±2.45 microns – 26.06 ±3.17 microns, in the distal section − 25.72±2.31 microns – 26.14± 2.93 microns (p≤0.05); the average wall thickness in the frontal section is 1.43± 0.09 microns - 1.48± 0.12 microns, in the distal section − 1.50± 0.11 microns – 1.54± 0.14 microns (p≤ 0.01). The vestibular surface of the maxillary medial incisors at the level of the root gingival part, as well as the oral surface at the root apical level have been found to be zones of periodontal ligament compression, while the oral surface at the root gingival level and the vestibular surface at the root apical level – zones of the periodontal ligament stretching. At the root gingival level of the first maxillary molars, the medial and vestibular surfaces are zones of the periodontal ligament compression, whereas the oral and the distal surfaces stand as the zones of the periodontal ligament stretching. As a quantitative assessment showed, the structural elements of the medial incisors periodontal ligament, and the first maxillary molars had loose connective tissue – taken by its specific area – prevailing in the periodontal ligament compression zone; the specific area of dense connective tissue, though, was found to be prevailing at the periodontal ligament stretching zone under chewing stress. The data obtained through a quantitative analysis of the pe
{"title":"HISTOMORPHOMETRIC ASSESSMENT OF ARCHITECTONICS AND VASCULARIZATION IN MAXILLARY ALVEOLAR PROCESS BONE TISSUE","authors":"D. Domenyuk, O. Sumkina, Natalya Mikutskaya, T. Kochkonyan, A. Markovsky, Dmitry Matsukatov, Yuri Harutyunyan, O. Ivanyuta, S. Domenyuk","doi":"10.35630/2023/13/3.308","DOIUrl":"https://doi.org/10.35630/2023/13/3.308","url":null,"abstract":"The expansion of the most relevant priority fields in fundamental and applied medicine, along with the progress in modern dentistry and reconstructive maxillofacial surgery, requires in-depth understanding of the morphological and functional status of the maxillary alveolar process bone tissue and knowledge of the mandibular alveolar part, thus allowing selecting a reasonable treatment tactics for maxillofacial pathologies. Qualitative features of maxillary alveolar process microarchitectonics, angioarchitectonics, as well as the periodontal ligament arrangement at various levels of the teeth roots, histological and morphometric studies of bone tissue in the frontal and distal sections, as well as the maxillary segments of the maxillary medial incisors and the first molars were studied in 5 certified male cadavers with preserved dentition. The histological findings show, that the microarchitectonics of the maxillary alveolar process features a lamellar bone consists of plates that are adjacent tightly to each other. The bone plates orientation in the frontal section is longitudinal, while in the distal part it is along concentric circles located around the Haversian canals. The angioarchitectonics of the maxillary alveolar process features tubular structures, which run mainly perpendicular to the bone surface, with numerous anastomoses. As the morphometric analysis of the maxillary alveolar process vascular system shows, in the frontal section the number of vessels per 1 mm2 is 22.41± 1.76 - 22.87± 2.08; in the distal section − 23.94± 1.88 - 25.02±2.69 (p≤0.01); the average diameter of vessels in the frontal section is 25.34±2.45 microns – 26.06 ±3.17 microns, in the distal section − 25.72±2.31 microns – 26.14± 2.93 microns (p≤0.05); the average wall thickness in the frontal section is 1.43± 0.09 microns - 1.48± 0.12 microns, in the distal section − 1.50± 0.11 microns – 1.54± 0.14 microns (p≤ 0.01). The vestibular surface of the maxillary medial incisors at the level of the root gingival part, as well as the oral surface at the root apical level have been found to be zones of periodontal ligament compression, while the oral surface at the root gingival level and the vestibular surface at the root apical level – zones of the periodontal ligament stretching. At the root gingival level of the first maxillary molars, the medial and vestibular surfaces are zones of the periodontal ligament compression, whereas the oral and the distal surfaces stand as the zones of the periodontal ligament stretching. As a quantitative assessment showed, the structural elements of the medial incisors periodontal ligament, and the first maxillary molars had loose connective tissue – taken by its specific area – prevailing in the periodontal ligament compression zone; the specific area of dense connective tissue, though, was found to be prevailing at the periodontal ligament stretching zone under chewing stress. The data obtained through a quantitative analysis of the pe","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46195987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One of the priority tasks of modern medicine is the search for new effective means that contribute to the treatment of burn injuries. In this area, metal complexes based on copper are of particular interest. The aim of the work is to evaluate the possible beneficial effect of a Cu(II) complex supported by acetylacetonates, [Cu(acac)2], on thermal burns by determining toxicity, analyzing survival and some cytogenetic and blood parameters. The metal complex [Cu(acac)2] turned out to be a low-toxic compound: the LD50/7 value was 1815 mg/kg. The study of survival, blood counts and cytogenetic parameters showed a beneficial effect of the [Cu(acac)2]. Studies have shown that in the early stages after the burn in animals injected with [Cu(acac)2], there is a significant decrease in blood clotting time (BCT) and hematocrit, an increase in the number of leukocytes. There is also a significant decrease in the levels of platelets, erythrocytes and hemoglobin. By the end of the experiment, there was a trend towards normalization of hematological parameters. The dynamics of the survival of animals from the group "burn + injections of the metal complex" with the corresponding regression curve and an equation that allows using extrapolation to predict the change in the percentage of survival in the long term of the experiment is given. The processes of wound healing, epithelialization, hair growth in the “burn + [Cu(acac)2]” group are more intense than in the pure burn group, which indicates the beneficial effect of this metal complex on the burned organism.
{"title":"INFLUENCE OF A COPPER(II) COMPLEX SUPPORTED BY ACETYLACETONATES ON SURVIVAL, CYTOGENETIC PARAMETERS AND BLOOD PARAMETERS OF ANIMALS WITH III-AB DEGREE BURNS","authors":"Anahit Karapetyan, Carlo Santini, Maura Pellei, Vahan Grigoryan, Ashot Dallakyan, Jasmina Petrosyan","doi":"10.35630/2023/13/5.502","DOIUrl":"https://doi.org/10.35630/2023/13/5.502","url":null,"abstract":"One of the priority tasks of modern medicine is the search for new effective means that contribute to the treatment of burn injuries. In this area, metal complexes based on copper are of particular interest. The aim of the work is to evaluate the possible beneficial effect of a Cu(II) complex supported by acetylacetonates, [Cu(acac)2], on thermal burns by determining toxicity, analyzing survival and some cytogenetic and blood parameters. The metal complex [Cu(acac)2] turned out to be a low-toxic compound: the LD50/7 value was 1815 mg/kg. The study of survival, blood counts and cytogenetic parameters showed a beneficial effect of the [Cu(acac)2]. Studies have shown that in the early stages after the burn in animals injected with [Cu(acac)2], there is a significant decrease in blood clotting time (BCT) and hematocrit, an increase in the number of leukocytes. There is also a significant decrease in the levels of platelets, erythrocytes and hemoglobin. By the end of the experiment, there was a trend towards normalization of hematological parameters. The dynamics of the survival of animals from the group \"burn + injections of the metal complex\" with the corresponding regression curve and an equation that allows using extrapolation to predict the change in the percentage of survival in the long term of the experiment is given. The processes of wound healing, epithelialization, hair growth in the “burn + [Cu(acac)2]” group are more intense than in the pure burn group, which indicates the beneficial effect of this metal complex on the burned organism.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135950149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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