The present study evaluates the effectiveness of the use of sulodexin (SDX) and ozone therapy in the treatment of lower limb varicose veins (classes C4 to C6) based on the study of the dynamics of plasma biomarkers of oxidative homeostasis and metabolomic profile. In patients of the comparison group, changes in the TAC index in blood plasma tended to increase, however, we didn’t established any statistical significance for the entire period of the study: initially - 305.5 ± 18.6 nmol/ml, then 322.3±17.7 nmol/ml on the day 20 after SI (surgical intervention), and 341.6±18.2 nmol/ml (p=0.672) on day 45 after SI. On the contrary, patients of the main group, by the day 20 after SI, had an increase in TAC level from 305.5±18.6 nmol/ml to 365.9±18.1 nmol/ml (by 19.8% of the baseline, p=0.046 ). By the day 20 after SI, in patients of the comparison group, the SOD activity in blood plasma tended to increase from 10.8±1.8 U/ml to 13.1±1.5 U/ml (by 21.3% relative to the baseline), but no statistical significance was found (p=0.733). The proposed method of complex treatment proved to be effective in correcting oxidative homeostasis and cellular metabolomic profile in patients with LLVV (classes C4 to C6).
{"title":"CORRECTION OF THE METABOLOMIC PROFILE AND REDOX POTENTIAL IN PATIENTS WITH LOWER LIMB VARICOSE VEINS (CLASS C4 - C6) IN THE POSTOPERATIVE PERIOD","authors":"Seyfeddine Khizriev, Vyacheslav Mykhaylichenko, Dmitry Parshin, Nadezhda Bondarenko, Sergey Samarin","doi":"10.35630/2023/13/5.504","DOIUrl":"https://doi.org/10.35630/2023/13/5.504","url":null,"abstract":"The present study evaluates the effectiveness of the use of sulodexin (SDX) and ozone therapy in the treatment of lower limb varicose veins (classes C4 to C6) based on the study of the dynamics of plasma biomarkers of oxidative homeostasis and metabolomic profile. In patients of the comparison group, changes in the TAC index in blood plasma tended to increase, however, we didn’t established any statistical significance for the entire period of the study: initially - 305.5 ± 18.6 nmol/ml, then 322.3±17.7 nmol/ml on the day 20 after SI (surgical intervention), and 341.6±18.2 nmol/ml (p=0.672) on day 45 after SI. On the contrary, patients of the main group, by the day 20 after SI, had an increase in TAC level from 305.5±18.6 nmol/ml to 365.9±18.1 nmol/ml (by 19.8% of the baseline, p=0.046 ). By the day 20 after SI, in patients of the comparison group, the SOD activity in blood plasma tended to increase from 10.8±1.8 U/ml to 13.1±1.5 U/ml (by 21.3% relative to the baseline), but no statistical significance was found (p=0.733). The proposed method of complex treatment proved to be effective in correcting oxidative homeostasis and cellular metabolomic profile in patients with LLVV (classes C4 to C6).","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136311657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The article presents and analyzes the effect of combined therapy of atorvastatin and essential phospholipids in patients with rheumatoid arthritis (RA) and lipid metabolism indicators in non-alcoholic fatty liver disease (NAFLD). We examined and treated 77 RA patients with NAFLD (64 women (83.1%) and 13 men (16.9%)), under the age of 55 years old. The patients were divided into 3 groups. Additionally to RA antirheumatic therapy, the first group (n=25) received atorvastatin, the second group (n=26) - essential phospholipids, and the third group (n=26) - a combination of atorvastatin and essential phospholipids for 6 months. Assessment of the state of lipid metabolism was carried out by studying the laboratory indices of a lipid profile. A hypolipidemic effect was revealed when using both atorvastatin and essential phospholipids in patients with RA NAFLD, which increases with their combined use. Whereas the levels of total cholesterol, triglycerides (TG), low-density lipoprotein (LDL) cholesterol, and very-low-density lipoprotein (VLDL) cholesterol levels in the blood serum significantly decrease with a simultaneous increase in the content of high-density lipoproteins (HDL) cholesterol. We revealed pronounced hypolipidemic and hepatoprotective effects in RA patients with NAFLD due to combined use of atorvastatin and essential phospholipids.
{"title":"EFFECT OF COMBINED THERAPY ON LIPID METABOLISM IN RHEUMATOID ARTHRITIS PATIENTS WITH AND NON-ALCOHOLIC FATTY LIVER DISEASE","authors":"Iryna Klymas","doi":"10.35630/2023/13/5.510","DOIUrl":"https://doi.org/10.35630/2023/13/5.510","url":null,"abstract":"The article presents and analyzes the effect of combined therapy of atorvastatin and essential phospholipids in patients with rheumatoid arthritis (RA) and lipid metabolism indicators in non-alcoholic fatty liver disease (NAFLD). We examined and treated 77 RA patients with NAFLD (64 women (83.1%) and 13 men (16.9%)), under the age of 55 years old. The patients were divided into 3 groups. Additionally to RA antirheumatic therapy, the first group (n=25) received atorvastatin, the second group (n=26) - essential phospholipids, and the third group (n=26) - a combination of atorvastatin and essential phospholipids for 6 months. Assessment of the state of lipid metabolism was carried out by studying the laboratory indices of a lipid profile. A hypolipidemic effect was revealed when using both atorvastatin and essential phospholipids in patients with RA NAFLD, which increases with their combined use. Whereas the levels of total cholesterol, triglycerides (TG), low-density lipoprotein (LDL) cholesterol, and very-low-density lipoprotein (VLDL) cholesterol levels in the blood serum significantly decrease with a simultaneous increase in the content of high-density lipoproteins (HDL) cholesterol. We revealed pronounced hypolipidemic and hepatoprotective effects in RA patients with NAFLD due to combined use of atorvastatin and essential phospholipids.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136311413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anahit Karapetyan, Vahan Grigoryan, Carlo Santini, Maura Pellei, Ashot Dallakyan
One of the priority tasks of modern radiobiology is the search for new, effective radioprotective compounds. In this area, metal complexes with high antioxidant activity are of particular interest. In order to study the possible favorable radioprotective effect of two copper complexes in different oxidation states, [Cu(PTA)4]BF4 and [Сu(acac)2], on the irradiated organism, we studied cytogenetic parameters in 4 groups of experimental animals. Group I included intact animals; Group II consisted of animals exposed to technetium (Tc) radioisotope, which were intraperitoneally injected with an isotope with an activity of 4.8 mCi in a volume of 2 mL - "pure irradiation"; Group III consisted of animals that were intraperitoneally injected with the copper(I) complex [Cu(PTA)4]BF4 at a dose of 50 mg/kg in a volume of 2 mL one hour before the administration of the Tc isotope (“irradiation + [Cu(PTA)4]BF4”). Group IV included animals that received the copper(II) complex [Сu(acac)2] before irradiation. We studied survival and cytogenetic parameters, determined the mitotic index, chromosomal aberrations and the percentage of polyploid cells. The survival rate of group II was 40%. In the group of intact animals, as well as in groups III and IV the survival rate was 100%. The dynamics of survival was described by regression equations, which make it possible, by means of extrapolation, to determine the change in the percentage of survival in the long term of the experiment and to predict the further outcome of the experiment. Investigating cytogenetic indicators, for all 3 indicators, a significant difference was found between intact and irradiated animals, i.e. these indicators can be considered as markers of technetium isotope exposure. In terms of proliferative activity, a significant difference was found in irradiated animals compared with groups III and IV, which indicates the radioprotective property of both the copper compounds. As for the [Cu(acac)2], when it was used in group IV and compared with others, we obtained a pronounced significant difference from the group with “pure irradiation” in all studied cytogenetic parameters. It was found that the copper complexes [Cu(PTA)4]BF4 and [Cu(acac)2] have the ability to prevent or mitigate the effect of ionizing radiation on an animal's body. Based on cytogenetic parameters, it can be concluded that these compounds promote reparative processes in the bone marrow cells of irradiated animals. Multiregression analysis of cytogenetic parameters confirmed the highest efficiency of the copper (II) complex [Cu(acac)2]. The results of the research indicate the need to continue work in the direction of searching for agents that have a therapeutic effect in radiation injuries.
现代放射生物学的首要任务之一是寻找新的、有效的辐射防护化合物。在这一领域，具有高抗氧化活性的金属配合物受到特别关注。为了研究两种不同氧化态的铜配合物[Cu(PTA)4]BF4和[Сu(acac)2]对受辐射生物体可能具有的有利辐射防护作用，我们对4组实验动物的细胞遗传学参数进行了研究。第一组为完整动物;II组为暴露于锝(Tc)放射性同位素的动物，腹腔注射活性为4.8 mCi的同位素，体积为2 mL -“纯照射”;第三组动物在给予Tc同位素(“照射+ [Cu(PTA)4]BF4”)前1小时，腹腔注射铜(I)配合物[Cu(PTA)4]BF4，剂量为50 mg/kg，体积为2 mL。IV组包括在辐照前接受铜(II)配合物[Сu(acac)2]的动物。我们研究了存活率和细胞遗传学参数，测定了有丝分裂指数、染色体畸变和多倍体细胞的百分比。II组生存率为40%。完整组及III、IV组成活率均为100%。生存的动态用回归方程来描述，通过外推，可以确定长期实验中生存百分比的变化，并预测实验的进一步结果。在细胞遗传学指标方面，完整动物和辐照动物的3项指标均存在显著差异，即这些指标可视为锝同位素暴露的标志。在增殖活性方面，与第三组和第四组相比，在辐照动物中发现了显着差异，这表明两种铜化合物都具有辐射防护性能。对于[Cu(acac)2]，在IV组使用时，与其他组相比，我们在所有研究的细胞遗传学参数上都与“纯照射”组有显著差异。发现铜配合物[Cu(PTA)4]BF4和[Cu(acac)2]具有预防或减轻电离辐射对动物身体影响的能力。基于细胞遗传学参数，可以得出结论，这些化合物促进了辐照动物骨髓细胞的修复过程。细胞遗传学参数的多元回归分析证实了铜(II)配合物[Cu(acac)2]的最高效率。研究结果表明，需要继续在寻找对辐射损伤有治疗作用的药物的方向上工作。
{"title":"INFLUENCE OF COPPER COMPLEXES [CU(PTA)4]BF4 AND [СU(ACAC)2] ON RATS IRRADIATED WITH RADIOISOTOPE TECHNETIUM","authors":"Anahit Karapetyan, Vahan Grigoryan, Carlo Santini, Maura Pellei, Ashot Dallakyan","doi":"10.35630/2023/13/5.501","DOIUrl":"https://doi.org/10.35630/2023/13/5.501","url":null,"abstract":"One of the priority tasks of modern radiobiology is the search for new, effective radioprotective compounds. In this area, metal complexes with high antioxidant activity are of particular interest. In order to study the possible favorable radioprotective effect of two copper complexes in different oxidation states, [Cu(PTA)4]BF4 and [Сu(acac)2], on the irradiated organism, we studied cytogenetic parameters in 4 groups of experimental animals. Group I included intact animals; Group II consisted of animals exposed to technetium (Tc) radioisotope, which were intraperitoneally injected with an isotope with an activity of 4.8 mCi in a volume of 2 mL - \"pure irradiation\"; Group III consisted of animals that were intraperitoneally injected with the copper(I) complex [Cu(PTA)4]BF4 at a dose of 50 mg/kg in a volume of 2 mL one hour before the administration of the Tc isotope (“irradiation + [Cu(PTA)4]BF4”). Group IV included animals that received the copper(II) complex [Сu(acac)2] before irradiation. We studied survival and cytogenetic parameters, determined the mitotic index, chromosomal aberrations and the percentage of polyploid cells. The survival rate of group II was 40%. In the group of intact animals, as well as in groups III and IV the survival rate was 100%. The dynamics of survival was described by regression equations, which make it possible, by means of extrapolation, to determine the change in the percentage of survival in the long term of the experiment and to predict the further outcome of the experiment. Investigating cytogenetic indicators, for all 3 indicators, a significant difference was found between intact and irradiated animals, i.e. these indicators can be considered as markers of technetium isotope exposure. In terms of proliferative activity, a significant difference was found in irradiated animals compared with groups III and IV, which indicates the radioprotective property of both the copper compounds. As for the [Cu(acac)2], when it was used in group IV and compared with others, we obtained a pronounced significant difference from the group with “pure irradiation” in all studied cytogenetic parameters. It was found that the copper complexes [Cu(PTA)4]BF4 and [Cu(acac)2] have the ability to prevent or mitigate the effect of ionizing radiation on an animal's body. Based on cytogenetic parameters, it can be concluded that these compounds promote reparative processes in the bone marrow cells of irradiated animals. Multiregression analysis of cytogenetic parameters confirmed the highest efficiency of the copper (II) complex [Cu(acac)2]. The results of the research indicate the need to continue work in the direction of searching for agents that have a therapeutic effect in radiation injuries.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":"64 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136234792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this work, the authors present a clinical case of a 65 year-old man with a history of three acute cerebrovascular accidents similar to a transient ischemic attack and/or ischemic stroke with a minor neurological deficit with short-term weakness in the left or right limbs and loss of coordination. Carotid angiography showed that the blood supply to the brain was provided by a single internal carotid artery on the left and a potent collateral network on the same side. The patient underwent stenting of the internal carotid artery on the left. As a result, blood supply was restored through the stented artery and pronounced collateralization on the right. This clinical case demonstrated that even in complex clinical situations associated with multifocal lesions of the carotid arteries, the use of x-ray endovascular technologies can achieve good clinical results, and x-ray-guided endovascular treatment of critical stenosis of the solitary internal carotid artery will allow achieving significant regression of neurological deficit and designating prognosis optima.
{"title":"X-RAY-GUIDED ENDOVASCULAR TREATMENT FOR CRITICAL STENOSIS OF A SOLITARY INTERNAL CAROTID ARTERY: A CLINICAL CASE","authors":"Vyacheslav Mykhaylichenko, Magomed Ahmadov, Dmitry Parshin, Rostislav Usupov, Alexey Gorbunov, Mariya Kalugina","doi":"10.35630/2023/13/5.505","DOIUrl":"https://doi.org/10.35630/2023/13/5.505","url":null,"abstract":"In this work, the authors present a clinical case of a 65 year-old man with a history of three acute cerebrovascular accidents similar to a transient ischemic attack and/or ischemic stroke with a minor neurological deficit with short-term weakness in the left or right limbs and loss of coordination. Carotid angiography showed that the blood supply to the brain was provided by a single internal carotid artery on the left and a potent collateral network on the same side. The patient underwent stenting of the internal carotid artery on the left. As a result, blood supply was restored through the stented artery and pronounced collateralization on the right. This clinical case demonstrated that even in complex clinical situations associated with multifocal lesions of the carotid arteries, the use of x-ray endovascular technologies can achieve good clinical results, and x-ray-guided endovascular treatment of critical stenosis of the solitary internal carotid artery will allow achieving significant regression of neurological deficit and designating prognosis optima.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":"289 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136312212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Serhiy Hryvenko, Yuriy Semenov, Alexander Golomidov, Andrej Dovgan, Tamila Usmanova, Vladimir Izosimov
The aim of this study was to experimentally validate the possibility and advisability of the treatment of polypropylene mesh implants with xenogenic cerebrospinal fluid in order to reduce a local acute inflammatory response. An experimental evaluation of the effectiveness of the proprietary method of polypropylene mesh implant processing during their implantation in the tissues of the anterior abdominal wall (Patent of Ukraine No. 146637) is presented. It has been proven that the use of the proposed new technology for the treatment of polypropylene mesh implants with a biocompatible component is a simple, safe and effective method that optimizes the reparative processes in the peri-implant zone without affecting the mechanical properties of mesh implants. All of the treatment effects were found to increase the biocompatibility and improve the survival rate of mesh implants. The thickness of the inflammatory ridge around the elements of mesh implants significantly decreased on average by 35.3% when xenogenic cerebrospinal fluid was used.
{"title":"EXPERIMENTAL SUBSTANTIATION OF THE USE OF XENOGENIC CEREBROSPINAL FLUID IN HERNIOLOGY TO REDUCE THE PERIPROSTHETIC REACTION","authors":"Serhiy Hryvenko, Yuriy Semenov, Alexander Golomidov, Andrej Dovgan, Tamila Usmanova, Vladimir Izosimov","doi":"10.35630/2023/13/5.503","DOIUrl":"https://doi.org/10.35630/2023/13/5.503","url":null,"abstract":"The aim of this study was to experimentally validate the possibility and advisability of the treatment of polypropylene mesh implants with xenogenic cerebrospinal fluid in order to reduce a local acute inflammatory response. An experimental evaluation of the effectiveness of the proprietary method of polypropylene mesh implant processing during their implantation in the tissues of the anterior abdominal wall (Patent of Ukraine No. 146637) is presented. It has been proven that the use of the proposed new technology for the treatment of polypropylene mesh implants with a biocompatible component is a simple, safe and effective method that optimizes the reparative processes in the peri-implant zone without affecting the mechanical properties of mesh implants. All of the treatment effects were found to increase the biocompatibility and improve the survival rate of mesh implants. The thickness of the inflammatory ridge around the elements of mesh implants significantly decreased on average by 35.3% when xenogenic cerebrospinal fluid was used.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":"30 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136312539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-12DOI: 10.35630/2022-2023/12/sp.iss.ed2
Konrad Siwek
Research objective: The aim of this article is to illustrate how prostheses are made, the significance of strength analyses, and why they are produced in accordance with ISO standards. Methodology: Research methods employed include literature review and analysis of an original research. Main conclusions: CAD design and strength analyses are crucial aspects of creating prostheses as medical devices. Modern technology allows for tailoring the design to a specific case and the patient's needs, considering anatomy and specific functional requirements. Numerical analyses make it possible to identify the most vulnerable elements to potential damage and optimize these areas through geometry modifications, among other factors. Additionally, it is possible to simulate critical conditions in which the designed element may find itself. An essential part of the research is conducting strength analyses according to ISO standards, which precisely specify how they should be carried out and under what load. Successfully passing these stages ensures legal safety for the designer and the possibility of implementing the designed solutions throughout the European Union. Application of the study: Strength analyses allow for predicting the behavior of prostheses under load and real-world usage conditions, which is crucial for patient safety. The use of CAD design enables customization of medical devices to individual patient needs. Originality/Novelty of the study: The presented results provide insights into the prostheses design process. By outlining the creation process and conducting numerical analyses, the complexity of this issue becomes apparent. Designing prostheses requires collaboration among several scientific disciplines, including biomechanics, engineering, medicine, materials science, and computer science.
{"title":"THE PROCESS OF LOWER LIMB PROSTHESIS CREATION USING CAD DESIGN AND FINITE ELEMENT ANALYSIS","authors":"Konrad Siwek","doi":"10.35630/2022-2023/12/sp.iss.ed2","DOIUrl":"https://doi.org/10.35630/2022-2023/12/sp.iss.ed2","url":null,"abstract":"Research objective: The aim of this article is to illustrate how prostheses are made, the significance of strength analyses, and why they are produced in accordance with ISO standards. Methodology: Research methods employed include literature review and analysis of an original research. Main conclusions: CAD design and strength analyses are crucial aspects of creating prostheses as medical devices. Modern technology allows for tailoring the design to a specific case and the patient's needs, considering anatomy and specific functional requirements. Numerical analyses make it possible to identify the most vulnerable elements to potential damage and optimize these areas through geometry modifications, among other factors. Additionally, it is possible to simulate critical conditions in which the designed element may find itself. An essential part of the research is conducting strength analyses according to ISO standards, which precisely specify how they should be carried out and under what load. Successfully passing these stages ensures legal safety for the designer and the possibility of implementing the designed solutions throughout the European Union. Application of the study: Strength analyses allow for predicting the behavior of prostheses under load and real-world usage conditions, which is crucial for patient safety. The use of CAD design enables customization of medical devices to individual patient needs. Originality/Novelty of the study: The presented results provide insights into the prostheses design process. By outlining the creation process and conducting numerical analyses, the complexity of this issue becomes apparent. Designing prostheses requires collaboration among several scientific disciplines, including biomechanics, engineering, medicine, materials science, and computer science.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":"91 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136012792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Antohe, C. Dascălu, D. Forna, E. Hitruc, N. Cimpoesu, C. Iordache, N. Forna
Aim: The aim of the study is to identify the causes of fractures of partially mobile skeletal prostheses, to determine the technological register or the design aspects of the prosthetic solution according to the particularity of the clinical case. Methods: A total of 56 fractured, partially removable skeletal prostheses were analyzed by AFM (Atomic Force Microscopy) between 2019 and 2023 to determine the structural changes in the fractured area compared to the other areas of the prosthesis. The surface images were obtained with a Solver PRO-M scanning probe microscope (NT-MDT), in AFM configuration. Results: According to the results in the literature by comparing the fatigue limit on different criteria it was revealed that the surface roughness was a significant factor in the material performance and with a high influence on the fatigue strength of the specimen. The surface roughness in the fracture zone, is much higher than the surfaces in the areas that resisted external stresses. Conclusions: Usually, the failure of a metal material in operation is due to a combination of factors, which are found in the technological register or may be generated by the particularities of the clinical case that have not been taken into account.
{"title":"FRACTURE ANALYSIS OF SKELETAL REMOVABLE PARTIAL DENTURES - EQUATION WITH SEVERAL UNKNOWNS","authors":"M. Antohe, C. Dascălu, D. Forna, E. Hitruc, N. Cimpoesu, C. Iordache, N. Forna","doi":"10.35630/2023/13/4.825","DOIUrl":"https://doi.org/10.35630/2023/13/4.825","url":null,"abstract":"Aim: The aim of the study is to identify the causes of fractures of partially mobile skeletal prostheses, to determine the technological register or the design aspects of the prosthetic solution according to the particularity of the clinical case. Methods: A total of 56 fractured, partially removable skeletal prostheses were analyzed by AFM (Atomic Force Microscopy) between 2019 and 2023 to determine the structural changes in the fractured area compared to the other areas of the prosthesis. The surface images were obtained with a Solver PRO-M scanning probe microscope (NT-MDT), in AFM configuration. Results: According to the results in the literature by comparing the fatigue limit on different criteria it was revealed that the surface roughness was a significant factor in the material performance and with a high influence on the fatigue strength of the specimen. The surface roughness in the fracture zone, is much higher than the surfaces in the areas that resisted external stresses. Conclusions: Usually, the failure of a metal material in operation is due to a combination of factors, which are found in the technological register or may be generated by the particularities of the clinical case that have not been taken into account.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41379750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Atefe Podineh, M. Rahnama, Abdolghani Abdollahimohammad, E. A. Bidmeshki, Aziz Shahraki Wahed
ntroduction: Emergency medical technicians, being the first-line medical team in emergency situations, experience higher levels of anxiety. On one hand, strict regulations regarding the use and management of protective equipment and specialized trainings on COVID-19 patients can reduce anxiety resulting from perceived unfamiliarity and uncontrollable risks. On the other hand, the Health Belief Model is one of the effective models in health education. Therefore, this study aimed to determine the impact of teaching COVID-19 coping protocols based on the Health Belief Model on infection preventive behaviors and anxiety among pre-hospital emergency technicians. Methods: In this quasi-experimental study, 40 technicians who expressed their willingness to participate were selected through convenience sampling and assigned to experimental (n=20) and control (n=20) groups using random allocation (via random allocation of bases). The data collection tools included a demographic questionnaire, the COVID-19 anxiety scale, and a self-developed questionnaire on infection preventive behaviors based on the Health Belief Model, which were completed before and one month after the intervention. During the intervention, educational content was delivered through e-mail or WhatsApp in four sessions. SPSS version 25 was used for data analysis. Results: According to the Bonferroni test, a significant difference was observed in the mean total score of infection preventive behaviors based on the Health Belief Model and all its dimensions. However, according to the independent t-test, there was no significant difference in anxiety scores between the two groups after the intervention. Conclusion: Based on the findings of this study, the educational intervention based on the Health Belief Model did not have an impact on anxiety among pre-hospital emergency technicians in dealing with COVID-19. However, the study identified the efficacy of all dimensions of this model in observing preventive behaviors against COVID-19 in these technicians. In other words, by applying this model, there is an opportunity to improve awareness and understanding of risk factors and the benefits of behavior change. This, in turn, helps in overcoming barriers, enhancing self-efficacy, and improving preventive behaviors. Therefore, it is recommended to utilize this model in designing preventive programs to increase the likelihood of the intervention success.
{"title":"USING HEALTH BELIEF MODEL IN COVID-19 TEACHING PROTOCOLS FOR PRE-HOSPITAL EMERGENCY TECHNICIANS:IMPACT ON INFECTION PREVENTIVE BEHAVIORS AND ANXIETY","authors":"Atefe Podineh, M. Rahnama, Abdolghani Abdollahimohammad, E. A. Bidmeshki, Aziz Shahraki Wahed","doi":"10.35630/2023/13/4.804","DOIUrl":"https://doi.org/10.35630/2023/13/4.804","url":null,"abstract":"ntroduction: Emergency medical technicians, being the first-line medical team in emergency situations, experience higher levels of anxiety. On one hand, strict regulations regarding the use and management of protective equipment and specialized trainings on COVID-19 patients can reduce anxiety resulting from perceived unfamiliarity and uncontrollable risks. On the other hand, the Health Belief Model is one of the effective models in health education. Therefore, this study aimed to determine the impact of teaching COVID-19 coping protocols based on the Health Belief Model on infection preventive behaviors and anxiety among pre-hospital emergency technicians. Methods: In this quasi-experimental study, 40 technicians who expressed their willingness to participate were selected through convenience sampling and assigned to experimental (n=20) and control (n=20) groups using random allocation (via random allocation of bases). The data collection tools included a demographic questionnaire, the COVID-19 anxiety scale, and a self-developed questionnaire on infection preventive behaviors based on the Health Belief Model, which were completed before and one month after the intervention. During the intervention, educational content was delivered through e-mail or WhatsApp in four sessions. SPSS version 25 was used for data analysis. Results: According to the Bonferroni test, a significant difference was observed in the mean total score of infection preventive behaviors based on the Health Belief Model and all its dimensions. However, according to the independent t-test, there was no significant difference in anxiety scores between the two groups after the intervention. Conclusion: Based on the findings of this study, the educational intervention based on the Health Belief Model did not have an impact on anxiety among pre-hospital emergency technicians in dealing with COVID-19. However, the study identified the efficacy of all dimensions of this model in observing preventive behaviors against COVID-19 in these technicians. In other words, by applying this model, there is an opportunity to improve awareness and understanding of risk factors and the benefits of behavior change. This, in turn, helps in overcoming barriers, enhancing self-efficacy, and improving preventive behaviors. Therefore, it is recommended to utilize this model in designing preventive programs to increase the likelihood of the intervention success.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42993131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Romanova, Pavel Dhzuvalyakov, E. Barinov, T. Chochlova
Baclofen (also sold under trade names Lioresal, Baclosan) belongs to a group of drugs called antispasmodics. This drug is available in oral and intrathecal forms. Baclofen has a pronounced psychotropic effect and is often a subject to abuse, especially among young people. The aim of the study was to detect apoptosis in the bronchial epithelium in rats. The study was performed on 20 male Wistar rats (weight: 290-350 g., age: 20 weeks). The animals were divided into 4 groups. The group of controls included 5 intact rats, each of three experimental groups included 5 rats treated with baclofen at a dose of 85 mg/kg and euthanized by the displacement of the cervical vertebrae after 3, 4,5, and 24 hours, respectively. In the bronchial epithelium of the controls bax was weakly expressed, which shows a low level of apoptosis. 3 and 4,5 hours after baclofen administration bax was strongly expressed and 24 hours after baclofen administration bax was moderately expressed. In the bronchial epithelium of the controls bcl-2 was weakly expressed; 3 hours after baclofen administration bcl-2 was moderately expressed; 4,5 hours and 24 hours after baclofen administration bcl-2 was strongly expressed. The results from the present study suggest the involvement of apoptosis in the development in the lesion of bronchial epithelium. Furthermore, bax and bcl-2 expression in the regenerating epithelial cells may play a role in the recovery of this lesion. Keywords: baclofen, poisoning, immunohistichemistry (IHC), apoptosis, bcl-2, bax.
{"title":"IMMUNOCHEMICAL ANALYSIS OF BCL-2 AND BAX EXPRESSION IN BRONCHIAL EPITHELIUM IN RATS IN ACUTE BACLOFEN POISONING","authors":"O. Romanova, Pavel Dhzuvalyakov, E. Barinov, T. Chochlova","doi":"10.35630/2023/13/4.806","DOIUrl":"https://doi.org/10.35630/2023/13/4.806","url":null,"abstract":"Baclofen (also sold under trade names Lioresal, Baclosan) belongs to a group of drugs called antispasmodics. This drug is available in oral and intrathecal forms. Baclofen has a pronounced psychotropic effect and is often a subject to abuse, especially among young people. The aim of the study was to detect apoptosis in the bronchial epithelium in rats. The study was performed on 20 male Wistar rats (weight: 290-350 g., age: 20 weeks). The animals were divided into 4 groups. The group of controls included 5 intact rats, each of three experimental groups included 5 rats treated with baclofen at a dose of 85 mg/kg and euthanized by the displacement of the cervical vertebrae after 3, 4,5, and 24 hours, respectively. In the bronchial epithelium of the controls bax was weakly expressed, which shows a low level of apoptosis. 3 and 4,5 hours after baclofen administration bax was strongly expressed and 24 hours after baclofen administration bax was moderately expressed. In the bronchial epithelium of the controls bcl-2 was weakly expressed; 3 hours after baclofen administration bcl-2 was moderately expressed; 4,5 hours and 24 hours after baclofen administration bcl-2 was strongly expressed. The results from the present study suggest the involvement of apoptosis in the development in the lesion of bronchial epithelium. Furthermore, bax and bcl-2 expression in the regenerating epithelial cells may play a role in the recovery of this lesion. Keywords: baclofen, poisoning, immunohistichemistry (IHC), apoptosis, bcl-2, bax.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42158813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Reva, Tatsuo Yamamoto, E. Mozhilevskaya, Pavel Zhibanov, Evgeni Bondar, Julia Gainulina, Shahariar Islam, G. Reva
Cells of non-hereditary retinblastoma in the enucleated eye of a 9-year-old boy were studied using classical histological and immunohistochemical methods. It has been established that disturbances in retinal morphogenesis can be associated with a stop in development at the stage of formation of retinal layers. The inner wall of the eyecup after the eviction of the neuroglia retained proliferative activity in the cells of the outer retinal layer, which lost the ability to differentiate over a larger extent of the retina and migrate to the inner layers. Cells that have differentiated according to the program of neuronal development are located among malignant cells and are characterized by signs of atypical aging with granules of black-brown pigment in the cytoplasm. It has been established that in the lack of angiogenesis these cells in the inner layers of the retina have secretory activity and are immunohistochemically phenotyped as VEGF-positive cells. This indicates cellular and tissue adaptation to malignancy at the terminal stage of retinoblastoma, which grows into the optic nerve and metastasizes to the brain. VEGF-positive cells can serve as one of the key targets in solving the problem of conservative treatment of retinoblastoma diagnosed in the late stages of the disease.
{"title":"AGING RETINAL NEURONS IN RETINOBLASTOMA","authors":"I. Reva, Tatsuo Yamamoto, E. Mozhilevskaya, Pavel Zhibanov, Evgeni Bondar, Julia Gainulina, Shahariar Islam, G. Reva","doi":"10.35630/2023/13/4.818","DOIUrl":"https://doi.org/10.35630/2023/13/4.818","url":null,"abstract":"Cells of non-hereditary retinblastoma in the enucleated eye of a 9-year-old boy were studied using classical histological and immunohistochemical methods. It has been established that disturbances in retinal morphogenesis can be associated with a stop in development at the stage of formation of retinal layers. The inner wall of the eyecup after the eviction of the neuroglia retained proliferative activity in the cells of the outer retinal layer, which lost the ability to differentiate over a larger extent of the retina and migrate to the inner layers. Cells that have differentiated according to the program of neuronal development are located among malignant cells and are characterized by signs of atypical aging with granules of black-brown pigment in the cytoplasm. It has been established that in the lack of angiogenesis these cells in the inner layers of the retina have secretory activity and are immunohistochemically phenotyped as VEGF-positive cells. This indicates cellular and tissue adaptation to malignancy at the terminal stage of retinoblastoma, which grows into the optic nerve and metastasizes to the brain. VEGF-positive cells can serve as one of the key targets in solving the problem of conservative treatment of retinoblastoma diagnosed in the late stages of the disease.","PeriodicalId":51770,"journal":{"name":"Archiv EuroMedica","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49433485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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