Pub Date : 2023-01-02DOI: 10.1080/2154896x.2023.2205247
Alice Oates, Johanna Grabow
{"title":"Report on the 2022 SCAR meetings: 10th SCAR open science conference and XXXVII SCAR delegates’ meeting","authors":"Alice Oates, Johanna Grabow","doi":"10.1080/2154896x.2023.2205247","DOIUrl":"https://doi.org/10.1080/2154896x.2023.2205247","url":null,"abstract":"","PeriodicalId":52117,"journal":{"name":"Polar Journal","volume":"13 1","pages":"169 - 171"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46475836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-02DOI: 10.1080/2154896X.2023.2205241
Pedro Marques-Quinteiro, Gloria Leon
ABSTRACT Psychological adaptation in isolated, confined, and extreme environments plays an important role in the achievement of performance and wellbeing. Whereas empirical research has been fruitful in determining the antecedents of psychological adaptation, opportunities remain to identify and apply new constructs through which psychological adaptation can grow. In the current study, we explored the possibility that one such construct is individual self-leadership. Participants were eight Antarctic scientists enrolled in the 2021–2022 summer campaign in the South Shetland Islands, under considerable COVID-19 restrictions during that period. Data were collected on three occasions and analysed through a mixed-method approach where qualitative and quantitative evidence were integrated. Overall, the results indicated that the self-imposed quarantine pre-deployment period in Punta Arenas had a heavy toll on expeditioners mental health, with the psychological adaptation and self-leadership of expeditioners being positively correlated across the campaign. The implications of our findings, as well possible future research directions, are discussed.
{"title":"Psychological adaptation in Antarctica amidst the COVID-19 pandemic","authors":"Pedro Marques-Quinteiro, Gloria Leon","doi":"10.1080/2154896X.2023.2205241","DOIUrl":"https://doi.org/10.1080/2154896X.2023.2205241","url":null,"abstract":"ABSTRACT Psychological adaptation in isolated, confined, and extreme environments plays an important role in the achievement of performance and wellbeing. Whereas empirical research has been fruitful in determining the antecedents of psychological adaptation, opportunities remain to identify and apply new constructs through which psychological adaptation can grow. In the current study, we explored the possibility that one such construct is individual self-leadership. Participants were eight Antarctic scientists enrolled in the 2021–2022 summer campaign in the South Shetland Islands, under considerable COVID-19 restrictions during that period. Data were collected on three occasions and analysed through a mixed-method approach where qualitative and quantitative evidence were integrated. Overall, the results indicated that the self-imposed quarantine pre-deployment period in Punta Arenas had a heavy toll on expeditioners mental health, with the psychological adaptation and self-leadership of expeditioners being positively correlated across the campaign. The implications of our findings, as well possible future research directions, are discussed.","PeriodicalId":52117,"journal":{"name":"Polar Journal","volume":"13 1","pages":"105 - 125"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48917778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-02DOI: 10.1080/2154896X.2023.2205248
L. Mercer, Kimberly L. Ovitz
Arctic research is progressing towards research in collaborative partnership with as opposed to on or in Arctic Indigenous communities (Henri, Martinez-Levasseur, Provencher, Debets, Appaqaq Houde 2022; Wong, Ballegooyen, Ignace, Johnson, Swanson, Gùdia Swanson 2020; ITK 2018). These collaborative, often cross-cultural partnerships are more equitable and impactful and have proven to be particularly effective at generating actionable knowledge that can enhance evidence-based decision making (Henri, Martinez-Levasseur, Provencher, Debets, Appaqaq Houde 2022) In recent years funding bodies and governance institutions have emphasized the need to build Indigenous community capacity to conduct Arctic research, however, this framing fails to acknowledge the crucial skills, unique place-based knowledge, and research capabilities that Arctic Indigenous peoples already possess and actively contribute towards research processes. As such, we argue for a shift away from capacity building (a one-way and top-down information flow often involving training Arctic residents in western science tools and methods) towards capacity sharing (a two-way exchange process that builds on Indigenous and western science knowledge and practices). Capacity sharing is developed from a foundation of reciprocity, communication, and collaboration and involves multi-directional knowledge exchange between research partners. At present, this terminology is applied across many research spheres yet there is no prevailing universal definition for capacity sharing, which leads to confusion and uncertainty (AOS 2022). We refine the definition of capacity sharing in the context of collaborative and community-engaged research and identify the elements critical to this process and to successfully transitioning from capacity building to capacity sharing in Arctic research. In December 2022, we facilitated a conference session on capacity sharing in collaborative research occurring in Inuit Nunangat at the ArcticNet Annual Scientific Meeting in Toronto, Canada (Archambault, Dawson, Barnard, Baird, Demers,
{"title":"Shifting from capacity building to capacity sharing in Arctic research: Considering transformative shifts in collaborative research at the ArcticNet Annual Scientific Meeting","authors":"L. Mercer, Kimberly L. Ovitz","doi":"10.1080/2154896X.2023.2205248","DOIUrl":"https://doi.org/10.1080/2154896X.2023.2205248","url":null,"abstract":"Arctic research is progressing towards research in collaborative partnership with as opposed to on or in Arctic Indigenous communities (Henri, Martinez-Levasseur, Provencher, Debets, Appaqaq Houde 2022; Wong, Ballegooyen, Ignace, Johnson, Swanson, Gùdia Swanson 2020; ITK 2018). These collaborative, often cross-cultural partnerships are more equitable and impactful and have proven to be particularly effective at generating actionable knowledge that can enhance evidence-based decision making (Henri, Martinez-Levasseur, Provencher, Debets, Appaqaq Houde 2022) In recent years funding bodies and governance institutions have emphasized the need to build Indigenous community capacity to conduct Arctic research, however, this framing fails to acknowledge the crucial skills, unique place-based knowledge, and research capabilities that Arctic Indigenous peoples already possess and actively contribute towards research processes. As such, we argue for a shift away from capacity building (a one-way and top-down information flow often involving training Arctic residents in western science tools and methods) towards capacity sharing (a two-way exchange process that builds on Indigenous and western science knowledge and practices). Capacity sharing is developed from a foundation of reciprocity, communication, and collaboration and involves multi-directional knowledge exchange between research partners. At present, this terminology is applied across many research spheres yet there is no prevailing universal definition for capacity sharing, which leads to confusion and uncertainty (AOS 2022). We refine the definition of capacity sharing in the context of collaborative and community-engaged research and identify the elements critical to this process and to successfully transitioning from capacity building to capacity sharing in Arctic research. In December 2022, we facilitated a conference session on capacity sharing in collaborative research occurring in Inuit Nunangat at the ArcticNet Annual Scientific Meeting in Toronto, Canada (Archambault, Dawson, Barnard, Baird, Demers,","PeriodicalId":52117,"journal":{"name":"Polar Journal","volume":"13 1","pages":"172 - 176"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45135359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-02DOI: 10.1080/2154896X.2023.2205243
K. Gormley, E. Hague, C. Andvik, Valentina DaCosta, Abigail Davies, D. Diz, K. Alexander, L. McWhinnie
ABSTRACT To support the predicted growth of shipping activities in the Arctic region in coming decades, port developments and associated shipping infrastructure will be required to be developed in both Arctic and sub-Arctic areas. Such large-scale development in unique and potentially vulnerable areas are likely to have wide-ranging effects and associated impacts. We therefore consider the future challenges, opportunities and knowledge gaps associated with the environmental impacts of developing Arctic and sub-Arctic port infrastructure. Here we present the outputs of an international, virtual workshop held in January 2022 exploring this theme. The workshop brought together Arctic, marine and port researchers, practitioners, non-governmental organisations, and local communities representing a range of geographies and disciplines. Based on pre-workshop consultation, five topics were considered: marine mammals and noise; discharges and pollution; ecosystem impacts and effects; environmental management and assessment; and infrastructure and geography. Dissemination of the workshop found five overriding themes that were common across each topic discussion: i) utilising best practice and governance; ii) community and Indigenous Peoples engagement and participation; iii) common vs. Arctic-specific challenges; iv) impact assessment including consideration of cumulative impacts and effects; and v) climate change. The workshop highlighted the requirement to continue to build and broaden discussion, for further collaborative work and research streams to be developed, to ensure any future Arctic and sub-Arctic port infrastructure, in support of Arctic shipping, is developed sustainably.
{"title":"First port of call: a horizon scanning workshop for sustainable Arctic marine infrastructure","authors":"K. Gormley, E. Hague, C. Andvik, Valentina DaCosta, Abigail Davies, D. Diz, K. Alexander, L. McWhinnie","doi":"10.1080/2154896X.2023.2205243","DOIUrl":"https://doi.org/10.1080/2154896X.2023.2205243","url":null,"abstract":"ABSTRACT To support the predicted growth of shipping activities in the Arctic region in coming decades, port developments and associated shipping infrastructure will be required to be developed in both Arctic and sub-Arctic areas. Such large-scale development in unique and potentially vulnerable areas are likely to have wide-ranging effects and associated impacts. We therefore consider the future challenges, opportunities and knowledge gaps associated with the environmental impacts of developing Arctic and sub-Arctic port infrastructure. Here we present the outputs of an international, virtual workshop held in January 2022 exploring this theme. The workshop brought together Arctic, marine and port researchers, practitioners, non-governmental organisations, and local communities representing a range of geographies and disciplines. Based on pre-workshop consultation, five topics were considered: marine mammals and noise; discharges and pollution; ecosystem impacts and effects; environmental management and assessment; and infrastructure and geography. Dissemination of the workshop found five overriding themes that were common across each topic discussion: i) utilising best practice and governance; ii) community and Indigenous Peoples engagement and participation; iii) common vs. Arctic-specific challenges; iv) impact assessment including consideration of cumulative impacts and effects; and v) climate change. The workshop highlighted the requirement to continue to build and broaden discussion, for further collaborative work and research streams to be developed, to ensure any future Arctic and sub-Arctic port infrastructure, in support of Arctic shipping, is developed sustainably.","PeriodicalId":52117,"journal":{"name":"Polar Journal","volume":"13 1","pages":"146 - 162"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48400838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-02DOI: 10.1080/2154896X.2023.2205237
M. Haward, A. Jackson
ABSTRACT The success of the Antarctic Treaty regime over more than 60 years provides the opportunity to assess the relationship between the maintenance of national interests and cooperation between Antarctic states. Over these six decades challenges to the Antarctic Treaty and the evolving Antarctic Treaty System have emerged. These challenges have arisen from within the system itself, most notably in the overturing of the proposed convention to regulate mining and its replacement with a comprehensive environmental protection regime. The system has also faced external challenges; a good example being through the “Question of Antarctica” debate within the United Nations General Assembly. Internal and external challenges have been addressed and, in many ways contributed to revitalising the regime, through adroit diplomacy and enduring commitment to sustaining the Treaty’s principles. This provides the key to the paper’s central argument that system resilience and adaptability has been undervalued in contemporary framings of Antarctic geopolitics that have in recent years tended to highlight tensions and divergent views.
{"title":"Antarctica: geopolitical challenges and institutional resilience","authors":"M. Haward, A. Jackson","doi":"10.1080/2154896X.2023.2205237","DOIUrl":"https://doi.org/10.1080/2154896X.2023.2205237","url":null,"abstract":"ABSTRACT The success of the Antarctic Treaty regime over more than 60 years provides the opportunity to assess the relationship between the maintenance of national interests and cooperation between Antarctic states. Over these six decades challenges to the Antarctic Treaty and the evolving Antarctic Treaty System have emerged. These challenges have arisen from within the system itself, most notably in the overturing of the proposed convention to regulate mining and its replacement with a comprehensive environmental protection regime. The system has also faced external challenges; a good example being through the “Question of Antarctica” debate within the United Nations General Assembly. Internal and external challenges have been addressed and, in many ways contributed to revitalising the regime, through adroit diplomacy and enduring commitment to sustaining the Treaty’s principles. This provides the key to the paper’s central argument that system resilience and adaptability has been undervalued in contemporary framings of Antarctic geopolitics that have in recent years tended to highlight tensions and divergent views. ","PeriodicalId":52117,"journal":{"name":"Polar Journal","volume":"13 1","pages":"31 - 48"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47814274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-02DOI: 10.1080/2154896X.2023.2205239
Mathieu Boulègue
ABSTRACT Geopolitical tension in the Antarctic is not a new feature, and the Antarctic Treaty System (ATS) is not about to crumble.1 Nevertheless, the region is slowly shaping to become a ‘contested space’2 in the presence of Russia and China. Taken individually and together, Moscow’s and Beijing’s postures and actions in Antarctic affairs are reshuffling the cards of good governance and consensus-based decisions in the ATS. Of particular interest is the relationship both countries have towards Antarctic affairs and the challenge they represent individually and jointly for the Five Eyes and its intelligence community. This article examines the respective Antarctic strategies of Russia and China. It then considers both Moscow and Beijing’s assessment of the Antarctic Treaty System in terms of its utility and durability. The article compares and contrasts the two states approaches to Antarctica to consider the implications for Five Eyes (FVEY) intelligence community. It concludes that within FVEY countries, keeping unity and coherence within the ATS is the best way to contain Russia’s and China’s regional ambitions – provided necessary measures are in place to keep track of their activities there.
{"title":"Five Eyes strategic interests in Antarctica: implications of contemporary Russian and Chinese strategy","authors":"Mathieu Boulègue","doi":"10.1080/2154896X.2023.2205239","DOIUrl":"https://doi.org/10.1080/2154896X.2023.2205239","url":null,"abstract":"ABSTRACT Geopolitical tension in the Antarctic is not a new feature, and the Antarctic Treaty System (ATS) is not about to crumble.1 Nevertheless, the region is slowly shaping to become a ‘contested space’2 in the presence of Russia and China. Taken individually and together, Moscow’s and Beijing’s postures and actions in Antarctic affairs are reshuffling the cards of good governance and consensus-based decisions in the ATS. Of particular interest is the relationship both countries have towards Antarctic affairs and the challenge they represent individually and jointly for the Five Eyes and its intelligence community. This article examines the respective Antarctic strategies of Russia and China. It then considers both Moscow and Beijing’s assessment of the Antarctic Treaty System in terms of its utility and durability. The article compares and contrasts the two states approaches to Antarctica to consider the implications for Five Eyes (FVEY) intelligence community. It concludes that within FVEY countries, keeping unity and coherence within the ATS is the best way to contain Russia’s and China’s regional ambitions – provided necessary measures are in place to keep track of their activities there.","PeriodicalId":52117,"journal":{"name":"Polar Journal","volume":"13 1","pages":"71 - 85"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46296300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-02DOI: 10.1080/2154896X.2023.2205242
Sonia Pedro Sebastiao
ABSTRACT This article aims to highlight the importance of raising awarenessabout the Arctic’s destruction and its consequences for humankind;sustaining that awareness can be promoted by rhetoricaldocumentaries. Awareness is a necessary departing point to fostersustainable citizenship and change behaviours. This change isneeded to preserve the Arctic and tackle global warming. Theauthor uses a humanistic interpretive paradigm to demonstrate thecontribution of the rhetorical documentary “The Last Ice” (2020)to raise awareness about the destruction of the Arctic, a naturalglobal common. The main conclusion pinpoints the media's role inrecognizing the need to listen, cooperate, and affect policy-making,turning it into a more unbiased and inclusive process since theyallow awareness raising among citizens. Rhetorical documentariesmay be a valuable resource to combine means to make theirvoice globally heard, enforcing the United Nations Declaration onthe Rights of Indigenous Peoples (2007) and active sustainablecitizenship.
{"title":"Rhetorical documentaries and the Arctic: raising awareness about the destruction of a natural global common","authors":"Sonia Pedro Sebastiao","doi":"10.1080/2154896X.2023.2205242","DOIUrl":"https://doi.org/10.1080/2154896X.2023.2205242","url":null,"abstract":"ABSTRACT This article aims to highlight the importance of raising awarenessabout the Arctic’s destruction and its consequences for humankind;sustaining that awareness can be promoted by rhetoricaldocumentaries. Awareness is a necessary departing point to fostersustainable citizenship and change behaviours. This change isneeded to preserve the Arctic and tackle global warming. Theauthor uses a humanistic interpretive paradigm to demonstrate thecontribution of the rhetorical documentary “The Last Ice” (2020)to raise awareness about the destruction of the Arctic, a naturalglobal common. The main conclusion pinpoints the media's role inrecognizing the need to listen, cooperate, and affect policy-making,turning it into a more unbiased and inclusive process since theyallow awareness raising among citizens. Rhetorical documentariesmay be a valuable resource to combine means to make theirvoice globally heard, enforcing the United Nations Declaration onthe Rights of Indigenous Peoples (2007) and active sustainablecitizenship.","PeriodicalId":52117,"journal":{"name":"Polar Journal","volume":"13 1","pages":"126 - 145"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47017652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-02DOI: 10.1080/2154896X.2023.2205249
Annette Löf, Zdenka Sokolíčková, N. Steins
ABSTRACT What makes an experience sustainable? Can polar cruise tourism in the High Arctic ever be sustainable? If so, under what conditions? These questions guide this report as it explores tensions embedded in the concept of ‘sustainable polar cruise tourism’. These matters are of particular importance in Svalbard, with a rapidly developing tourism sector being an important part of the Svalbard economy. The report is based on a participatory workshop held on-board a recent expedition to the Svalbard archipelago, the so-called SEES expedition (the Scientific Expedition Edgeøya Svalbard). Conducted as a combined scientific and touristic expedition, the SEES expedition offers opportunity to explore the meaning(s) of sustainability among environmentally aware visitors representing key actor groups on Svalbard. The purpose of the report is thus to contribute to ongoing conversations on the paradoxes of sustainable polar tourism by sharing experiences and perspectives on what sustainability can mean from a passenger point of view.
{"title":"Experiencing Svalbard sustainably? Reflecting on what we can learn about polar cruise tourism from the SEES expedition","authors":"Annette Löf, Zdenka Sokolíčková, N. Steins","doi":"10.1080/2154896X.2023.2205249","DOIUrl":"https://doi.org/10.1080/2154896X.2023.2205249","url":null,"abstract":"ABSTRACT What makes an experience sustainable? Can polar cruise tourism in the High Arctic ever be sustainable? If so, under what conditions? These questions guide this report as it explores tensions embedded in the concept of ‘sustainable polar cruise tourism’. These matters are of particular importance in Svalbard, with a rapidly developing tourism sector being an important part of the Svalbard economy. The report is based on a participatory workshop held on-board a recent expedition to the Svalbard archipelago, the so-called SEES expedition (the Scientific Expedition Edgeøya Svalbard). Conducted as a combined scientific and touristic expedition, the SEES expedition offers opportunity to explore the meaning(s) of sustainability among environmentally aware visitors representing key actor groups on Svalbard. The purpose of the report is thus to contribute to ongoing conversations on the paradoxes of sustainable polar tourism by sharing experiences and perspectives on what sustainability can mean from a passenger point of view.","PeriodicalId":52117,"journal":{"name":"Polar Journal","volume":"13 1","pages":"177 - 185"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43377253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-16eCollection Date: 2022-01-01DOI: 10.1155/2022/4509019
Longping Ye, Youqing Zhong, Lihua Hu, Ya Huang, Xiang Tang, Shanjun Yu, Jianxin Huang, Ziyuan Wang, Qi Li, Xiangdong Zhou
Objective: This study was aimed at investigating the expression level of hsa_circ_0061817 in lung adenocarcinoma cells and its effect on cell proliferation and invasion and the possible mechanism of hsa_circ_0061817 in lung adenocarcinoma.
Methods: The overexpression plasmids of hsa_circ_0061817 (OE-hsacirc_0061817) were transfected into human lung A549 cells and mouse LLC-LUC cells, respectively. The cell viability was detected by CCK-8, and the cell proliferation was detected by cell clone formation assay and EdU assay. Transwell test was used to detect the ability of cell invasion, and apoptosis was detected by flow cytometry. WB was applied to determine the expression of apoptosis and epithelial mesenchymal transition- (EMT-) related proteins and also target proteins for observation the effect of OE-hsa_circ_0061817 on the growth of A549 cells in nude mice. Bioinformatics method was used to predict the binding microRNA (miRNA) of hsa_circ_0061817 and construct the regulatory network of competitive endogenous RNA (ceRNA) and functional analysis of miRNA target genes.
Results: Compared with PLO-ciR group, the cell viability, proliferation, and invasive ability of A549 and LLC-LUC were significantly reduced in OE-hsa_circ_00061817 group, while the apoptosis increased in OE-hsa_circ_00061817 group compared to PLO-ciR group. WB results showed that the expression of caspase 3, caspase 7, caspase 9, and E-cadherin increased significantly, while the expression levels of vimentin and N-cadherin decreased severely. Most importantly, OE-hsa_circ_00061817 inhibited the growth of A549 tumor-bearing nude mice. According to TargetScan and mirBase databases, hsa_circ_0061817 may competitively bind hsa_mir-181b-3p, hsa-mir-337-3p, hsa-mir-421, and hsa-mir-548d-3p. The results of functional enrichment showed that miRNA target genes were involved in many cancer-related biological processes, including negative regulation of apoptosis, gene expression, transcriptional imbalance in cancer, transforming growth factor-β, and P53 signal pathway.
Conclusions: Over expression of hsa_circ_0061817 inhibits the proliferation of lung adenocarcinoma A549 and LLC-LUC cells and may reduce the invasive ability of lung adenocarcinoma cells by weakening the process of EMT, which provides a new target for the prevention and treatment of lung adenocarcinoma.
{"title":"Overexpression of hsa_circ_0061817 Can Inhibit the Proliferation and Invasion of Lung Cancer Cells Based on Active Compounds.","authors":"Longping Ye, Youqing Zhong, Lihua Hu, Ya Huang, Xiang Tang, Shanjun Yu, Jianxin Huang, Ziyuan Wang, Qi Li, Xiangdong Zhou","doi":"10.1155/2022/4509019","DOIUrl":"10.1155/2022/4509019","url":null,"abstract":"<p><strong>Objective: </strong>This study was aimed at investigating the expression level of hsa_circ_0061817 in lung adenocarcinoma cells and its effect on cell proliferation and invasion and the possible mechanism of hsa_circ_0061817 in lung adenocarcinoma.</p><p><strong>Methods: </strong>The overexpression plasmids of hsa_circ_0061817 (OE-hsacirc_0061817) were transfected into human lung A549 cells and mouse LLC-LUC cells, respectively. The cell viability was detected by CCK-8, and the cell proliferation was detected by cell clone formation assay and EdU assay. Transwell test was used to detect the ability of cell invasion, and apoptosis was detected by flow cytometry. WB was applied to determine the expression of apoptosis and epithelial mesenchymal transition- (EMT-) related proteins and also target proteins for observation the effect of OE-hsa_circ_0061817 on the growth of A549 cells in nude mice. Bioinformatics method was used to predict the binding microRNA (miRNA) of hsa_circ_0061817 and construct the regulatory network of competitive endogenous RNA (ceRNA) and functional analysis of miRNA target genes.</p><p><strong>Results: </strong>Compared with PLO-ciR group, the cell viability, proliferation, and invasive ability of A549 and LLC-LUC were significantly reduced in OE-hsa_circ_00061817 group, while the apoptosis increased in OE-hsa_circ_00061817 group compared to PLO-ciR group. WB results showed that the expression of caspase 3, caspase 7, caspase 9, and E-cadherin increased significantly, while the expression levels of vimentin and N-cadherin decreased severely. Most importantly, OE-hsa_circ_00061817 inhibited the growth of A549 tumor-bearing nude mice. According to TargetScan and mirBase databases, hsa_circ_0061817 may competitively bind hsa_mir-181b-3p, hsa-mir-337-3p, hsa-mir-421, and hsa-mir-548d-3p. The results of functional enrichment showed that miRNA target genes were involved in many cancer-related biological processes, including negative regulation of apoptosis, gene expression, transcriptional imbalance in cancer, transforming growth factor-<i>β</i>, and P53 signal pathway.</p><p><strong>Conclusions: </strong>Over expression of hsa_circ_0061817 inhibits the proliferation of lung adenocarcinoma A549 and LLC-LUC cells and may reduce the invasive ability of lung adenocarcinoma cells by weakening the process of EMT, which provides a new target for the prevention and treatment of lung adenocarcinoma.</p>","PeriodicalId":52117,"journal":{"name":"Polar Journal","volume":"3 1","pages":"4509019"},"PeriodicalIF":0.0,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11401659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82117035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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