首页 > 最新文献

作物学报最新文献

英文 中文
Regulation of flavonoid synthesis by BjA09.TT8 and BjB08.TT8 genes in Brassica juncea BjA09.TT8和BjB08.TT8基因对芥菜类黄酮合成的调控
Pub Date : 2022-05-01 DOI: 10.3724/sp.j.1006.2022.14058
Wei Huang, Guo-Ying Gao, Jinzhu Wu, Li-li Liu, Da-wei Zhang, Ding-gang Zhou, Hong-tao Cheng, Kaifa Zhang, Mei-Liang Zhou, Mei Li, M. Yan
{"title":"Regulation of flavonoid synthesis by <italic>BjA09.TT8 </italic>and <italic>BjB08.TT8</italic> genes in <italic>Brassica juncea</italic>","authors":"Wei Huang, Guo-Ying Gao, Jinzhu Wu, Li-li Liu, Da-wei Zhang, Ding-gang Zhou, Hong-tao Cheng, Kaifa Zhang, Mei-Liang Zhou, Mei Li, M. Yan","doi":"10.3724/sp.j.1006.2022.14058","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2022.14058","url":null,"abstract":"","PeriodicalId":52132,"journal":{"name":"Acta Agronomica Sinica(China)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41508806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Drought resistance identification and drought resistance indexes screening of Tibetan hulless barley resources at adult stage 西藏无壳大麦资源成株期抗旱性鉴定及抗旱性指标筛选
Pub Date : 2022-05-01 DOI: 10.3724/sp.j.1006.2022.11048
Xingrong Wang, Yue Li, Yanjun Zhang, Yongsheng Li, Jun-Cheng Wang, Yin-Ping Xu, Xusheng Qi
: Drought is one of the main factors affecting the production of hulless barley in the Qinghai-Tibet Plateau. Identification of drought resistance of hulless barley germplasm resources at adult stage and screening of reliable drought resistance indexes can provide basis for breeding drought resistant varieties. In the present study, a total of 238 hulless barley germplasms were used as materials, and two treatments of normal irrigation and drought stress in the field conditions were conducted in Zhangye of Gansu Province in 2019 and 2020, respectively. Seven indexes, such as the plant height, spike number per plant, biomass per plant, grain weight per spike, spike length, 1000-grain weight, and yield were determined. The drought resistance comprehensive evaluation value ( D -value), comprehensive drought resistance coefficient (CDC-value), and weight drought resistance coefficient (WDC-value) were used to identify drought resistance at adult stage of hulless barley germplasms. The results showed that the effects of drought stress on all indexes were extremely significant, and the correlation analysis showed that the yield was significantly positively correlated with plant height and 1000-grain nificantly positively correlated with plant height and 1000-grain weight at P < 0.01, and significantly positively correlated with the grain weight per panicle at P <0.05. Frequency analysis showed that the sensitivity of seven indexes to drought stress was plant height, yield, biomass per plant, grain weight per ear, number of ears per plant, ear length and 1000-grain weight, respectively. The ranks of drought resistance of germplasms based on the D -value, CDC-value, and WDC-value were similar. Grey correlation degree analysis showed that the correlation degree between DC-value and D -value was in the order of yield, biomass per plant, grain weight per spike, plant height, number of spikelets per plant, ear length and 1000-grain weight, which was basically consis-tent with the close degree between DC-value and WDC-value of each index. Stepwise regression analysis showed that yield, plant height, panicle number per plant, 1000-grain weight and biomass per plant could be used as reliable indexes for drought resistance identification of hulless barley in adult stage. D -value was determined as the appropriate method for drought resistance identification. Based on D -value, the tested hulless barley germplasms were divided into five drought resistance grades, and 38 germplasms were classified in grade I, such as Ziyanwoqingke, Liulengqingke, Ziqingke, ZDM8125, Tuhuluqingke, etc. These results pro-vided important basic materials and theoretical reference for the breeding of drought-resistant varieties and the study of drought-resistant mechanism of hulless barley.
干旱是影响青藏高原大麦生产的主要因素之一。大麦成虫期种质资源抗旱性鉴定和可靠的抗旱指标筛选可为选育抗旱品种提供依据。本研究以甘肃张掖地区238份无壳大麦种质为材料,分别于2019年和2020年在大田条件下进行正常灌溉和干旱胁迫两种处理。测定了单株株高、单株穗数、单株生物量、单穗粒重、穗长、千粒重、产量等7个指标。采用抗旱性综合评价值(D -value)、综合抗旱性系数(CDC-value)和重量抗旱性系数(WDC-value)对大麦种质成虫期抗旱性进行了鉴定。结果表明,干旱胁迫对各指标的影响均极显著,相关分析表明,产量与株高呈极显著正相关,千粒重与株高、千粒重呈极显著正相关(P < 0.01),与每穗粒重呈极显著正相关(P <0.05)。频率分析表明,7个指标对干旱胁迫的敏感性分别为株高、产量、单株生物量、单穗粒重、单穗穗数、穗长和千粒重。基于D值、cdc值和wdc值的种质抗旱性排序相似。灰色关联度分析表明,dc值与D值的关联度依次为产量、单株生物量、单穗粒重、株高、单株颖花数、穗长、千粒重,与各指标dc值与wdc值的密切程度基本一致。逐步回归分析表明,产量、株高、单株穗数、千粒重和单株生物量可作为大麦成虫期抗旱性鉴定的可靠指标。确定D值作为抗旱性鉴定的合适方法。根据D值将所试无壳大麦种质划分为5个抗旱性等级,其中自盐窝青科、柳冷清科、自青科、ZDM8125、吐huluqingke等38个品种为1级。这些结果为脱壳大麦抗旱品种选育和抗旱机理研究提供了重要的基础资料和理论参考。
{"title":"Drought resistance identification and drought resistance indexes screening of Tibetan hulless barley resources at adult stage","authors":"Xingrong Wang, Yue Li, Yanjun Zhang, Yongsheng Li, Jun-Cheng Wang, Yin-Ping Xu, Xusheng Qi","doi":"10.3724/sp.j.1006.2022.11048","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2022.11048","url":null,"abstract":": Drought is one of the main factors affecting the production of hulless barley in the Qinghai-Tibet Plateau. Identification of drought resistance of hulless barley germplasm resources at adult stage and screening of reliable drought resistance indexes can provide basis for breeding drought resistant varieties. In the present study, a total of 238 hulless barley germplasms were used as materials, and two treatments of normal irrigation and drought stress in the field conditions were conducted in Zhangye of Gansu Province in 2019 and 2020, respectively. Seven indexes, such as the plant height, spike number per plant, biomass per plant, grain weight per spike, spike length, 1000-grain weight, and yield were determined. The drought resistance comprehensive evaluation value ( D -value), comprehensive drought resistance coefficient (CDC-value), and weight drought resistance coefficient (WDC-value) were used to identify drought resistance at adult stage of hulless barley germplasms. The results showed that the effects of drought stress on all indexes were extremely significant, and the correlation analysis showed that the yield was significantly positively correlated with plant height and 1000-grain nificantly positively correlated with plant height and 1000-grain weight at P < 0.01, and significantly positively correlated with the grain weight per panicle at P <0.05. Frequency analysis showed that the sensitivity of seven indexes to drought stress was plant height, yield, biomass per plant, grain weight per ear, number of ears per plant, ear length and 1000-grain weight, respectively. The ranks of drought resistance of germplasms based on the D -value, CDC-value, and WDC-value were similar. Grey correlation degree analysis showed that the correlation degree between DC-value and D -value was in the order of yield, biomass per plant, grain weight per spike, plant height, number of spikelets per plant, ear length and 1000-grain weight, which was basically consis-tent with the close degree between DC-value and WDC-value of each index. Stepwise regression analysis showed that yield, plant height, panicle number per plant, 1000-grain weight and biomass per plant could be used as reliable indexes for drought resistance identification of hulless barley in adult stage. D -value was determined as the appropriate method for drought resistance identification. Based on D -value, the tested hulless barley germplasms were divided into five drought resistance grades, and 38 germplasms were classified in grade I, such as Ziyanwoqingke, Liulengqingke, Ziqingke, ZDM8125, Tuhuluqingke, etc. These results pro-vided important basic materials and theoretical reference for the breeding of drought-resistant varieties and the study of drought-resistant mechanism of hulless barley.","PeriodicalId":52132,"journal":{"name":"Acta Agronomica Sinica(China)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42696924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of chromosome loss and rearrangement in potato and eggplant somatic hybrids by rDNA and telomere repeats 利用rDNA和端粒重复序列鉴定马铃薯和茄子体细胞杂交种染色体丢失和重排
Pub Date : 2022-05-01 DOI: 10.3724/sp.j.1006.2022.14070
Hai-bo Wang, Jingwen Ying, Li He, Wen-Xuan Ye, W. Tu, Xingkui Cai, B. Song, J. Liu
: Somatic hybridization is an important way to create new germplasm. Somatic hybrids produced plenty of genetic variation during protoplast regeneration. In this study, to analyze the chromosome composition and variation of potato and eggplant somatic hybrids, rDNAs and telomeric repeats were used as probes for FISH (fluorescence in situ hybridization), combined with GISH (Genomic in situ hybridization). The results showed that rearranged chromosomes and dicentric chromosomes existed in somatic hybrids, and the parts of the rearranged chromosomes was derived from the end-to-end fusion of potato and eggplant chromosomes 2. One centromere of the rearranged dicentric chromosomes was derived from potato and the other was from eggplant. Eggplant 5S rDNA sites were lost in somatic hybrids to homogenize the rDNA of somatic hybrids. The results of this study indicated that the chromosomes were unstable during the somatic hybridization of potato and eggplant, which can easily cause dicentric and chromosomal rearrangements in somatic hybrids. The chromosomes of somatic hybrids tended to be stable through various ways such as chromosome rearrangement, dicentric and rDNA homogenization.
体细胞杂交是创造新种质的重要途径。体细胞杂种在原生质体再生过程中产生了大量的遗传变异。在本研究中,为了分析马铃薯和茄子体细胞杂种的染色体组成和变异,使用rDNA和端粒重复序列作为FISH(荧光原位杂交)探针,并结合GISH(基因组原位杂交)。结果表明,体细胞杂种中存在重排染色体和双着丝粒染色体,重排染色体的部分来源于马铃薯和茄子2号染色体的端到端融合。重新排列的双着丝粒染色体的一个着丝粒来源于马铃薯,另一个来源于茄子。在体细胞杂种中丢失茄子5S rDNA位点以使体细胞杂种的rDNA均质化。本研究结果表明,马铃薯和茄子的体细胞杂交过程中染色体不稳定,容易导致体细胞杂交中的双着丝粒和染色体重排。体细胞杂种的染色体通过染色体重排、双着丝粒和rDNA均一化等多种途径趋于稳定。
{"title":"Identification of chromosome loss and rearrangement in potato and eggplant somatic hybrids by rDNA and telomere repeats","authors":"Hai-bo Wang, Jingwen Ying, Li He, Wen-Xuan Ye, W. Tu, Xingkui Cai, B. Song, J. Liu","doi":"10.3724/sp.j.1006.2022.14070","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2022.14070","url":null,"abstract":": Somatic hybridization is an important way to create new germplasm. Somatic hybrids produced plenty of genetic variation during protoplast regeneration. In this study, to analyze the chromosome composition and variation of potato and eggplant somatic hybrids, rDNAs and telomeric repeats were used as probes for FISH (fluorescence in situ hybridization), combined with GISH (Genomic in situ hybridization). The results showed that rearranged chromosomes and dicentric chromosomes existed in somatic hybrids, and the parts of the rearranged chromosomes was derived from the end-to-end fusion of potato and eggplant chromosomes 2. One centromere of the rearranged dicentric chromosomes was derived from potato and the other was from eggplant. Eggplant 5S rDNA sites were lost in somatic hybrids to homogenize the rDNA of somatic hybrids. The results of this study indicated that the chromosomes were unstable during the somatic hybridization of potato and eggplant, which can easily cause dicentric and chromosomal rearrangements in somatic hybrids. The chromosomes of somatic hybrids tended to be stable through various ways such as chromosome rearrangement, dicentric and rDNA homogenization.","PeriodicalId":52132,"journal":{"name":"Acta Agronomica Sinica(China)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42076173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptome analysis of leaves responses to elevated CO2 concentration, drought and interaction conditions in soybean [Glycine max (Linn.) Merr.] 大豆叶片对CO2浓度升高、干旱和互作条件响应的转录组分析[j]。稳定)
Pub Date : 2022-05-01 DOI: 10.3724/sp.j.1006.2022.14055
Ang Li, Ya-Nan Feng, Ping Li, Dong-sheng Zhang, Y. Zong, Wen-Zhong Lin, X. Hao
{"title":"Transcriptome analysis of leaves responses to elevated CO<sub>2</sub> concentration, drought and interaction conditions in soybean [<italic>Glycine max </italic>(Linn.) Merr.]","authors":"Ang Li, Ya-Nan Feng, Ping Li, Dong-sheng Zhang, Y. Zong, Wen-Zhong Lin, X. Hao","doi":"10.3724/sp.j.1006.2022.14055","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2022.14055","url":null,"abstract":"","PeriodicalId":52132,"journal":{"name":"Acta Agronomica Sinica(China)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47961735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Effects of drought hardening on contemporary expression of drought stress memory genes and DNA methylation in promoter of B73 inbred progeny 干旱胁迫对干旱胁迫记忆基因当代表达及B73近交系启动子DNA甲基化的影响
Pub Date : 2022-05-01 DOI: 10.3724/sp.j.1006.2022.13027
Xia Wang, Xiaohui Yin, Xiao-ming Yu, Xiao-Dan Liu
{"title":"Effects of drought hardening on contemporary expression of drought stress memory genes and DNA methylation in promoter of B73 inbred progeny","authors":"Xia Wang, Xiaohui Yin, Xiao-ming Yu, Xiao-Dan Liu","doi":"10.3724/sp.j.1006.2022.13027","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2022.13027","url":null,"abstract":"","PeriodicalId":52132,"journal":{"name":"Acta Agronomica Sinica(China)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47614838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characteristics of panicle stem growth and flowering period of the parents of hybrid rice in machine-transplanted seed production 机种生产中杂交稻亲本穗茎生长及花期特征
Pub Date : 2022-04-01 DOI: 10.3724/sp.j.1006.2022.12020
Qin Qin, Youde Tao, Bang-Chao Huang, Hui Li, Yun Gao, Xiao-yuan Zhong, Zhonglin Zhou, Li Zhu, X. Lei, Sheng-Qiang Feng, Xu Wang, W. Ren
: To study the characteristics of panicle and stem growth and flowering period of restorer line and sterile line under mechanized transplanting mode, and to analyze the reasons for the difficulty of parental flowering period meeting under mechanized transplanting mode Chenghui 727×Shu 21A and Yahui 2115×Yixiang 1A were selected as the materials, and the field ex-periment was carried out from 2019 to 2020 in this study. Two transplanting methods and seedling ages were determined. Leaf age dynamics, panicle stem growth dynamics, and heading dynamics of restorer line and sterile line were investigated, and the effects of different treatments on the growth and development of panicle, line were statistically analyzed. The results were as follows: (1) The total number of leaves in mechanical transplanting parents decreased by 0.3–0.8 leaves in restorer lines while the sterile line added 0.1–0.4 leaves. Meanwhile, the total number of leaves in restorer lines decreased with the decrease of seedling age, while there was no significant change in the total number of leaves of male sterile lines. (2) The growth process of panicles, stems and leaves of restorer lines in mechanical transplanting was delayed by 2–5 days, while that of sterile lines was delayed by 5–10 days. The panicle differentiation of restorer lines began after jointing, while the panicle differentiation of sterile lines began 5–10 days after jointing. The panicle differentiation duration of restorer lines and sterile lines did not change significantly. The young ear growth curves of all treated parents were well fitted with Logistic curves ( R 2 >0.99). (3) The duration from seeding to heading of machine transplanting parents was longer than that of artificial transplanting parents. The effect of lengthening seedling age was the same as the change of transplanting method and the response of sterile lines to transplanting method and seedling age was more significant than that of restorer lines. (4) The flower encounter index of long seedling age under artificial transplanting in each combination was 100%, and the flower encounter was the best. Flowering encountering index was between 40%–60% of long seedling age under mechanical transplanting and short seedling age under artificial transplanting, followed by flowering status. However, the flowering index of the short seedling age combination of Chenghui 727 × Shu 21A and Yahui 2115 × Yixiang 1A was 0 and 18.18% under mechanical transplanting, respectively, indicat-ing that the absent of flowering date seriously. In conclusion, the growth and development of ear, stem, and leaf of parents were delayed under mechanized transplanting mode, and the prolonged seedling age aggravated the growth and development lag phe-nomenon of parents. There were some differences in the growth, development, and flowering characteristics between restorer lines and sterile lines under different transplanting methods and seedling ages. The difference in the response
为研究机械化移栽模式下恢复系和不育系穗茎生长和花期特征,分析机械化移栽模式下亲本花期难以满足的原因,本研究选择成辉727×Shu 21A和雅辉2115×Yixiang 1A为材料,于2019 - 2020年进行田间试验。确定了两种移栽方式和苗龄。研究了恢复系和不育系的叶龄动态、穗茎生长动态和抽穗动态,并统计分析了不同处理对穗、系生长发育的影响。结果表明:(1)恢复系机械移栽亲本叶片总数减少0.3 ~ 0.8片,不育系增加0.1 ~ 0.4片;同时,恢复系叶片总数随苗龄的降低而减少,而雄性不育系叶片总数变化不显著。(2)机械移栽恢复系穗、茎、叶的生长过程延迟2 ~ 5 d,不育系延迟5 ~ 10 d。恢复系穗部分化在拔节后开始,不育系穗部分化在拔节后5 ~ 10 d开始。恢复系和不育系的穗分化期变化不显著。各处理亲本幼穗生长曲线均符合Logistic曲线(R = 0.99)。(3)机插亲本从播种期到抽穗期比人工移栽亲本长。延长苗龄的效果与移栽方式的变化相同,不育系对移栽方式和苗龄的反应比恢复系更显著。(4)人工插秧下各组合长苗龄的遇花指数均为100%,遇花效果最好。机械移栽苗龄长、人工移栽苗龄短的开花偶遇指数在40% ~ 60%之间,其次是开花状态。而成辉727 ×曙21A和亚辉2115 ×宜香1A短苗龄组合在机械移栽下的开花指数分别为0和18.18%,说明开花日期缺失严重。综上所述,机械化移栽方式使亲本穗、茎、叶的生长发育延迟,苗龄延长加剧了亲本生长发育滞后现象。在不同移栽方式和苗龄下,恢复系和不育系的生长发育和开花特性存在一定差异。恢复系和不育系对栽培措施的反应差异是造成亲本开花日期缺失的主要原因。
{"title":"Characteristics of panicle stem growth and flowering period of the parents of hybrid rice in machine-transplanted seed production","authors":"Qin Qin, Youde Tao, Bang-Chao Huang, Hui Li, Yun Gao, Xiao-yuan Zhong, Zhonglin Zhou, Li Zhu, X. Lei, Sheng-Qiang Feng, Xu Wang, W. Ren","doi":"10.3724/sp.j.1006.2022.12020","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2022.12020","url":null,"abstract":": To study the characteristics of panicle and stem growth and flowering period of restorer line and sterile line under mechanized transplanting mode, and to analyze the reasons for the difficulty of parental flowering period meeting under mechanized transplanting mode Chenghui 727×Shu 21A and Yahui 2115×Yixiang 1A were selected as the materials, and the field ex-periment was carried out from 2019 to 2020 in this study. Two transplanting methods and seedling ages were determined. Leaf age dynamics, panicle stem growth dynamics, and heading dynamics of restorer line and sterile line were investigated, and the effects of different treatments on the growth and development of panicle, line were statistically analyzed. The results were as follows: (1) The total number of leaves in mechanical transplanting parents decreased by 0.3–0.8 leaves in restorer lines while the sterile line added 0.1–0.4 leaves. Meanwhile, the total number of leaves in restorer lines decreased with the decrease of seedling age, while there was no significant change in the total number of leaves of male sterile lines. (2) The growth process of panicles, stems and leaves of restorer lines in mechanical transplanting was delayed by 2–5 days, while that of sterile lines was delayed by 5–10 days. The panicle differentiation of restorer lines began after jointing, while the panicle differentiation of sterile lines began 5–10 days after jointing. The panicle differentiation duration of restorer lines and sterile lines did not change significantly. The young ear growth curves of all treated parents were well fitted with Logistic curves ( R 2 >0.99). (3) The duration from seeding to heading of machine transplanting parents was longer than that of artificial transplanting parents. The effect of lengthening seedling age was the same as the change of transplanting method and the response of sterile lines to transplanting method and seedling age was more significant than that of restorer lines. (4) The flower encounter index of long seedling age under artificial transplanting in each combination was 100%, and the flower encounter was the best. Flowering encountering index was between 40%–60% of long seedling age under mechanical transplanting and short seedling age under artificial transplanting, followed by flowering status. However, the flowering index of the short seedling age combination of Chenghui 727 × Shu 21A and Yahui 2115 × Yixiang 1A was 0 and 18.18% under mechanical transplanting, respectively, indicat-ing that the absent of flowering date seriously. In conclusion, the growth and development of ear, stem, and leaf of parents were delayed under mechanized transplanting mode, and the prolonged seedling age aggravated the growth and development lag phe-nomenon of parents. There were some differences in the growth, development, and flowering characteristics between restorer lines and sterile lines under different transplanting methods and seedling ages. The difference in the response","PeriodicalId":52132,"journal":{"name":"Acta Agronomica Sinica(China)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44980089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Creation and identification of peanut germplasm tolerant to triazolopyrimidine herbicides 花生耐三唑嘧啶除草剂种质资源的创建与鉴定
Pub Date : 2022-04-01 DOI: 10.3724/sp.j.1006.2022.14066
Jiaxing Liu, Yu Lan, Qian Xu, Hong-ye Li, Xin-Yu Zhou, Xuan Zhao, Y. Gan, Hong-Bo Liu, Yueping Zheng, Y. Zhan, Gang Zhang, Zhijun Zheng
: At present, the germplasm resources of herbicide-tolerant peanut ( Arachis hypogaea ) are scarce, which restrict the di-versification of peanut-based cropping system. To create peanut germplasm with tolerance to different herbicides, a mutant popu-lation consisting of more than 55,000 peanut lines were generated by ethylmethanesulfonate mutagenesis. We screened this popu-lation with a variety of different herbicides to obtain multiple mutants with tolerance to different herbicides. One of these lines displayed strong tolerance to florasulam and flumetsulam in the experiments with foliar herbicide spraying under field conditions as well as in various laboratory evaluation for the herbicide tolerance, while the herbicide-tolerant trait did not have adverse effects on peanut yield and quality. To determine whether this trait was associated with a target-site-based resistance to the herbicides, we compared gene sequences and relative expression levels of these two acetohydroxyacid synthases (AHASs) as the herbicide target enzymes between the mutant and wild type. Molecular cloning and sequence analysis revealed that peanut chromosome A10 and B10 each contained a gene, named as AhAHAS1a and AhAHAS1b , which were highly similar to Arabidopsis AHAS . Peanut chromosome A08 and B08 also each carried an AHAS gene, named as AhAHAS2a and AhAHAS2b , respectively. However, compared with the wild type, mutant and wild type. In summary, these results indicate that the herbicide tolerance of the mutant might be caused by non-target-site-based resistance mechanism.
目前,耐除草剂花生(Arachis hypogaea)种质资源匮乏,制约了花生为主种植体系的多样化发展。为了培育耐除草剂的花生种质资源,采用甲基磺酸乙酯诱变方法,建立了5.5万多个花生品系的突变体群体。我们用多种不同的除草剂筛选该种群,获得对不同除草剂具有耐受性的多个突变体。其中1个品系对florasulam和flumetsulam表现出较强的耐受性,在田间条件下叶面喷施除草剂试验和各种室内除草剂耐受性评价中,耐除草剂性状对花生产量和品质没有不利影响。为了确定这一性状是否与基于靶点的除草剂抗性相关,我们比较了突变型和野生型作为除草剂靶酶的这两种乙酰羟基酸合成酶(AHASs)的基因序列和相对表达水平。分子克隆和序列分析表明,花生染色体A10和B10各含有一个基因,命名为AhAHAS1a和AhAHAS1b,与拟南芥AHAS高度相似。花生染色体A08和B08也分别携带一个AHAS基因,分别命名为AhAHAS2a和AhAHAS2b。然而,与野生型相比,突变型和野生型。综上所述,这些结果表明该突变体的除草剂耐受性可能是由非靶点抗性机制引起的。
{"title":"Creation and identification of peanut germplasm tolerant to triazolopyrimidine herbicides","authors":"Jiaxing Liu, Yu Lan, Qian Xu, Hong-ye Li, Xin-Yu Zhou, Xuan Zhao, Y. Gan, Hong-Bo Liu, Yueping Zheng, Y. Zhan, Gang Zhang, Zhijun Zheng","doi":"10.3724/sp.j.1006.2022.14066","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2022.14066","url":null,"abstract":": At present, the germplasm resources of herbicide-tolerant peanut ( Arachis hypogaea ) are scarce, which restrict the di-versification of peanut-based cropping system. To create peanut germplasm with tolerance to different herbicides, a mutant popu-lation consisting of more than 55,000 peanut lines were generated by ethylmethanesulfonate mutagenesis. We screened this popu-lation with a variety of different herbicides to obtain multiple mutants with tolerance to different herbicides. One of these lines displayed strong tolerance to florasulam and flumetsulam in the experiments with foliar herbicide spraying under field conditions as well as in various laboratory evaluation for the herbicide tolerance, while the herbicide-tolerant trait did not have adverse effects on peanut yield and quality. To determine whether this trait was associated with a target-site-based resistance to the herbicides, we compared gene sequences and relative expression levels of these two acetohydroxyacid synthases (AHASs) as the herbicide target enzymes between the mutant and wild type. Molecular cloning and sequence analysis revealed that peanut chromosome A10 and B10 each contained a gene, named as AhAHAS1a and AhAHAS1b , which were highly similar to Arabidopsis AHAS . Peanut chromosome A08 and B08 also each carried an AHAS gene, named as AhAHAS2a and AhAHAS2b , respectively. However, compared with the wild type, mutant and wild type. In summary, these results indicate that the herbicide tolerance of the mutant might be caused by non-target-site-based resistance mechanism.","PeriodicalId":52132,"journal":{"name":"Acta Agronomica Sinica(China)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43798690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials 马铃薯SSR引物开发、鉴定分析及有色马铃薯材料扩增研究
Pub Date : 2022-04-01 DOI: 10.3724/sp.j.1006.2022.14065
Xia Zhang, Zhuo Yu, Xing Jin, Xiaoxia Yu, Jingwei Li, Jiaqi Li
: Compared with commonly cultivated potato, colored potato is rich in nutrition, especially in anthocyanins, which is the focus of breeding research in recent years. To date, the number of potato SSR primers developed is limited, especially those related to colored potato. In this study, SSR loci were analyzed using MISA software based on the whole genome. The results were as follows: a total of 218,997 SSR loci were obtained from potato genome with an average of 3.39 kb. Mononucleotide was the main repeat type, accounting for 62.05% of the total SSR, followed by dinucleotide and trinucleotide, accounting for 22.39% and 13.11%, respectively. The repeats of the six nucleotide types ranged from 5 to 746, mainly from 5 to 10, accounting for 60.9% of the total SSRs. A total of 215 motif types were obtained in all detected SSRs. The dominant motifs of the other five nucleotide repeat types were mainly A/T-containing motifs, except for hexanucleotide repeat type. The length of SSR motifs ranged from 12 bp to 20 bp, accounting for 43.19% of all SSRs. A total of 100 SSR primers were designed by Primer 5 software, among which 48 primers were preliminarily screened using parents’ genomic DNA of the colored potato, with an effective amplification rate of 48%. Then, six F 2 individuals were randomly selected for PCR amplification, and 26 primers with clear and stable bands and high polymorphism were finely screened, with the average polymorphism rate of 72.52%. In summary, the number and types of SSR loci in potato genome was abundant and diverse, and the polymorphism was moderate. The developed primers were highly polymorphic, which can be used in the development of SSR markers, genetic diversity analysis, and SSR fingerprint of colored potato, providing a scientific basis for further mining genes related to anthocyanin content.
:与普通种植的马铃薯相比,彩色马铃薯营养丰富,尤其是花青素,是近年来育种研究的重点。迄今为止,开发的马铃薯SSR引物数量有限,特别是与彩色马铃薯有关的引物。本研究基于全基因组,利用MISA软件对SSR位点进行分析。结果表明:从马铃薯基因组中共获得218997个SSR位点,平均为3.39 kb;单核苷酸为主要重复类型,占SSR总数的62.05%,其次是二核苷酸和三核苷酸,分别占22.39%和13.11%。6种核苷酸类型的重复数在5 ~ 746之间,主要在5 ~ 10之间,占总SSRs的60.9%。在所有检测到的ssr中,共获得215个基序类型。除六核苷酸重复型外,其他5种核苷酸重复型的优势基序主要为含A/ t基序。SSR基序长度为12 ~ 20 bp,占全部SSR的43.19%。利用Primer 5软件设计了100条SSR引物,其中利用彩色马铃薯亲本基因组DNA初步筛选了48条引物,有效扩增率为48%。然后随机选取6个f2个体进行PCR扩增,筛选出条带清晰稳定、多态性高的引物26条,平均多态性率为72.52%。综上所述,马铃薯基因组中SSR位点数量和类型丰富多样,多态性适中。所构建的引物具有高度多态性,可用于彩色马铃薯的SSR标记开发、遗传多样性分析和SSR指纹图谱,为进一步挖掘花青素含量相关基因提供科学依据。
{"title":"Development and characterization analysis of potato SSR primers and the amplification research in colored potato materials","authors":"Xia Zhang, Zhuo Yu, Xing Jin, Xiaoxia Yu, Jingwei Li, Jiaqi Li","doi":"10.3724/sp.j.1006.2022.14065","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2022.14065","url":null,"abstract":": Compared with commonly cultivated potato, colored potato is rich in nutrition, especially in anthocyanins, which is the focus of breeding research in recent years. To date, the number of potato SSR primers developed is limited, especially those related to colored potato. In this study, SSR loci were analyzed using MISA software based on the whole genome. The results were as follows: a total of 218,997 SSR loci were obtained from potato genome with an average of 3.39 kb. Mononucleotide was the main repeat type, accounting for 62.05% of the total SSR, followed by dinucleotide and trinucleotide, accounting for 22.39% and 13.11%, respectively. The repeats of the six nucleotide types ranged from 5 to 746, mainly from 5 to 10, accounting for 60.9% of the total SSRs. A total of 215 motif types were obtained in all detected SSRs. The dominant motifs of the other five nucleotide repeat types were mainly A/T-containing motifs, except for hexanucleotide repeat type. The length of SSR motifs ranged from 12 bp to 20 bp, accounting for 43.19% of all SSRs. A total of 100 SSR primers were designed by Primer 5 software, among which 48 primers were preliminarily screened using parents’ genomic DNA of the colored potato, with an effective amplification rate of 48%. Then, six F 2 individuals were randomly selected for PCR amplification, and 26 primers with clear and stable bands and high polymorphism were finely screened, with the average polymorphism rate of 72.52%. In summary, the number and types of SSR loci in potato genome was abundant and diverse, and the polymorphism was moderate. The developed primers were highly polymorphic, which can be used in the development of SSR markers, genetic diversity analysis, and SSR fingerprint of colored potato, providing a scientific basis for further mining genes related to anthocyanin content.","PeriodicalId":52132,"journal":{"name":"Acta Agronomica Sinica(China)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45900395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of close planting densities on assimilate accumulation and yield of soybean with different plant branching types 密植密度对不同分枝类型大豆同化物积累和产量的影响
Pub Date : 2022-04-01 DOI: 10.3724/sp.j.1006.2022.14045
Rui-Dong Li, Yang Yin, Wenwen Song, Tingting Wu, Shi Sun, Tian-fu Han, Caifeng Xu, Cunxiang Wu, Shuixiu Hu
: To investigate the effects of planting density on leaf area index, dry matter accumulation and distribution, and yield components of different varieties, field experiments were conducted using two soybean varieties with different branching types (Zhongzuo XA12938, a main stem type variety; Zhonghuang 13, a branched type variety) under six planting densities (D1: 13.5×10 4 plants hm –2 ; D2: 18.0×10 4 plants hm –2 ; D3: 22.5×10 4 plants hm –2 ; D4: 13, respectively. Compared to Zhonghuang 13, the high LAI values in Zhongzuo XA12938 lasted longer and decreased less at the middle and late stages. The degree of increase in dry matter with density varied between treatments. At podding stage, dry weight increased by 77.53% and 51.21% in the high-density treatment (D6) compared to the low-density treatment (D1) for Zhongzuo XA12938 and Zhonghuang 13, respectively. The percentage of dry matter in reproductive organs at maturity stage increased and then decreased with increasing density. The highest yields were achieved under D5 (5000.45 kg hm –2 ) treatment and remained stable at increasing densities for Zhongzuo XA12938. The highest yields for both years were achieved under D4 (4477.90 kg hm –2 ) and D5 (3935.30 kg hm –2 ) treatments for Zhonghuang 13. The average yield of Zhongzuo XA12938 was significantly higher by 22.37% than that of Zhonghuang 13. Grey correlation analysis revealed that plant height and effective pods per unit area were closely related to yield in Zhongzuo XA12938, while effective grains per unit area and height of centre of gravity were more highly correlated with yield in Zhonghuang 13. Zhongzuo XA12938 moderate increase in density can increase the LAI and pro-long the duration of its high value, promote dry matter accumulation, increase the proportion of reproductive organs, and improve the yield in soybean. Varieties with strong meristem regulation can be used to improve yields and increase benefits by appropri-ately increasing planting density in production.
:研究种植密度对不同品种叶面积指数、干物质积累和分布以及产量构成的影响,采用两个不同分枝类型的大豆品种(中作XA12938,主茎型品种;中黄13,分枝型品种)在六种种植密度下进行了田间试验(D1:13.5×104株hm-2;D2:18.0×104株hm2;D3:22.5×104植株hm-2;D4:13。与中黄13相比,中左XA12938的高LAI持续时间更长,中后期下降幅度较小。不同处理间干物质随密度的增加程度不同-与中佐XA12938和中黄13的低密度处理(D1)相比。生殖器官成熟期干物质含量随密度的增加先增加后减少。中作XA12938在D5(5000.45 kg hm–2)处理下产量最高,并在密度增加时保持稳定。中黄13号在D4(4477.90 kg hm–2)和D5(3935.30 kg hm2–2)处理下产量最高。中作XA12938的平均产量比中黄13显著提高22.37%。灰色关联分析表明,中作XA12938的株高和单位面积有效荚数与产量密切相关,而中黄13的单位面积有效粒数和重心高度与产量的相关性更高。中佐XA12938适度增加密度可以提高大豆的LAI,延长其高值持续时间,促进干物质积累,增加生殖器官比例,提高产量。具有较强分生组织调控能力的品种可在生产中通过适当增加种植密度来提高产量和效益。
{"title":"Effects of close planting densities on assimilate accumulation and yield of soybean with different plant branching types","authors":"Rui-Dong Li, Yang Yin, Wenwen Song, Tingting Wu, Shi Sun, Tian-fu Han, Caifeng Xu, Cunxiang Wu, Shuixiu Hu","doi":"10.3724/sp.j.1006.2022.14045","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2022.14045","url":null,"abstract":": To investigate the effects of planting density on leaf area index, dry matter accumulation and distribution, and yield components of different varieties, field experiments were conducted using two soybean varieties with different branching types (Zhongzuo XA12938, a main stem type variety; Zhonghuang 13, a branched type variety) under six planting densities (D1: 13.5×10 4 plants hm –2 ; D2: 18.0×10 4 plants hm –2 ; D3: 22.5×10 4 plants hm –2 ; D4: 13, respectively. Compared to Zhonghuang 13, the high LAI values in Zhongzuo XA12938 lasted longer and decreased less at the middle and late stages. The degree of increase in dry matter with density varied between treatments. At podding stage, dry weight increased by 77.53% and 51.21% in the high-density treatment (D6) compared to the low-density treatment (D1) for Zhongzuo XA12938 and Zhonghuang 13, respectively. The percentage of dry matter in reproductive organs at maturity stage increased and then decreased with increasing density. The highest yields were achieved under D5 (5000.45 kg hm –2 ) treatment and remained stable at increasing densities for Zhongzuo XA12938. The highest yields for both years were achieved under D4 (4477.90 kg hm –2 ) and D5 (3935.30 kg hm –2 ) treatments for Zhonghuang 13. The average yield of Zhongzuo XA12938 was significantly higher by 22.37% than that of Zhonghuang 13. Grey correlation analysis revealed that plant height and effective pods per unit area were closely related to yield in Zhongzuo XA12938, while effective grains per unit area and height of centre of gravity were more highly correlated with yield in Zhonghuang 13. Zhongzuo XA12938 moderate increase in density can increase the LAI and pro-long the duration of its high value, promote dry matter accumulation, increase the proportion of reproductive organs, and improve the yield in soybean. Varieties with strong meristem regulation can be used to improve yields and increase benefits by appropri-ately increasing planting density in production.","PeriodicalId":52132,"journal":{"name":"Acta Agronomica Sinica(China)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48029748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Identification and analysis of RNA editing sites of chloroplast genes in foxtail millet [Setaria italica (L.) P. Beauv.] 谷子叶绿体基因RNA编辑位点的鉴定与分析
Pub Date : 2022-04-01 DOI: 10.3724/sp.j.1006.2022.14043
Xi Du, Zhilan Wang, Kangni Han, Shichao Lian, Yuxin Li, Lin-Yi Zhang, Jun Wang
RNA editing is one of the post-transcriptional regulation mechanisms of gene expression in the chloroplast genomes of higher plants, which affects the chloroplast development and leads to albino phenotype or yellow phenotype of plant leaves. In this study, chlorophyll content was measured with a UV spectrophotometer at seeding stage among Changnong 35, E752, and E1005, and chloroplast structure of leaves was observed with a transmission electron microscopy; the online tool Prep-CP was
RNA编辑是高等植物叶绿体基因组中基因表达的转录后调控机制之一,它影响叶绿体的发育,导致植物叶片的白化表型或黄色表型。本研究用紫外分光光度计测定了长农35、E752和E1005在播种期的叶绿素含量,并用透射电子显微镜观察了叶片的叶绿体结构;在线工具Prep CP
{"title":"Identification and analysis of RNA editing sites of chloroplast genes in foxtail millet [<italic>Setaria italica</italic> (L.) P. Beauv.]","authors":"Xi Du, Zhilan Wang, Kangni Han, Shichao Lian, Yuxin Li, Lin-Yi Zhang, Jun Wang","doi":"10.3724/sp.j.1006.2022.14043","DOIUrl":"https://doi.org/10.3724/sp.j.1006.2022.14043","url":null,"abstract":"RNA editing is one of the post-transcriptional regulation mechanisms of gene expression in the chloroplast genomes of higher plants, which affects the chloroplast development and leads to albino phenotype or yellow phenotype of plant leaves. In this study, chlorophyll content was measured with a UV spectrophotometer at seeding stage among Changnong 35, E752, and E1005, and chloroplast structure of leaves was observed with a transmission electron microscopy; the online tool Prep-CP was","PeriodicalId":52132,"journal":{"name":"Acta Agronomica Sinica(China)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44416929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
作物学报
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1