Water Research X最新文献

The safe management of fecal sludge from the 3.4 billion people worldwide that use onsite sanitation systems can greatly reduce the global infectious disease burden. However, there is limited knowledge about the role of design, operational, and environmental factors on pathogen survival in pit latrines, urine diverting desiccation toilets, and other types of onsite toilets. We conducted a systematic literature review and meta-analysis to characterize pathogen reduction rates in fecal sludge, feces, and human excreta with respect to pH, temperature, moisture content, and the use of additives for desiccation, alkalinization, or disinfection. A meta-analysis of 1,382 data points extracted from 243 experiments described in 26 articles revealed significant differences between the decay rates and T₉₉ values of pathogens and indicators from different microbial groups. The overall median T₉₉ values were 4.8 days, 29 days, >341 days, and 429 days for bacteria, viruses, protozoan (oo)cysts, and Ascaris eggs, respectively. As expected, higher pH values, higher temperatures, and the application of lime all significantly predicted greater pathogen reduction rates but the use of lime by itself was more effective for bacteria and viruses than for Ascaris eggs, unless urea was also added. In multiple lab-scale experiments, the application of urea with enough lime or ash to reach a pH of 10 – 12 and a sustained concentration of 2,000 – 6,000 mg/L of non-protonated NH₃N reduced Ascaris eggs more rapidly than without urea. In general, the storage of fecal sludge for 6 months adequately controls hazards from viruses and bacteria, but much longer storage times or alkaline treatment with urea and low moisture or heat is needed to control hazards from protozoa and helminths. More research is needed to demonstrate the efficacy of lime, ash, and urea in the field. More studies of protozoan pathogens are also needed, as very few qualifying experiments were found for this group.

Acid-tolerant ammonia-oxidizing bacteria (AOB) can open the door to new applications, such as partial nitritation at low pH. However, they can also be problematic because chemical nitrite oxidation occurs at low pH, leading to the release of harmful nitrogen oxide gases. In this publication, the role of acid-tolerant AOB in urine treatment was explored. On the one hand, the technical feasibility of ammonia oxidation under acidic conditions for source-separated urine with total nitrogen concentrations up to 3.5 g-N L⁻¹ was investigated. On the other hand, the abundance and growth of acid-tolerant AOB at more neutral pH was explored. Under acidic conditions (pH of 5), ammonia oxidation rates of 500 mg-N L⁻¹ d⁻¹ and 10 g-N g-VSS^-1 d^-1 were observed, despite high concentrations of 15 mg-N L⁻¹ of the AOB-inhibiting compound nitrous acid and low concentration of 0.04 mg-N L⁻¹ of the substrate ammonia. However, ammonia oxidation under acidic conditions was very sensitive to process disturbances. Even short periods of less than 12 h without oxygen or without influent resulted in a complete cessation of ammonia oxidation with a recovery time of up to two months, which is a problem for low maintenance applications such as decentralized treatment. Furthermore, undesirable nitrogen losses of about 10% were observed. Under acidic conditions, a novel AOB strain was enriched with a relative abundance of up to 80%, for which the name “Candidatus (Ca.) Nitrosacidococcus urinae” is proposed. While Nitrosacidococcus members were present only to a small extent (0.004%) in urine nitrification reactors operated at pH values between 5.8 and 7, acid-tolerant AOB were always enriched during long periods without influent, resulting in an uncontrolled drop in pH to as low as 2.5. Long-term experiments at different pH values showed that the activity of “Ca. Nitrosacidococcus urinae” decreased strongly at a pH of 7, where they were also outcompeted by the acid-sensitive AOB Nitrosomonas halophila. The experiment results showed that the decreased activity of “Ca. Nitrosacidococcus urinae” correlated with the limited availability of dissolved iron at neutral pH.

The underlying chemistry of weak-base (WB) anion exchange resins (AERs) for contaminant removal from water is not well documented in the literature. To address this, batch adsorption experiments were conducted at pH 4, 7, and 10 using two representative WB-AERs (polyacrylic IRA67 and polystyrene IRA96) and two representative strong-base (SB) AERs (polyacrylic IRA458 and polystyrene A520E), of differing polymer composition, for the removal of nitrate, sulfate, 3-phenylpropionic acid (3-PPA) as surrogate for natural organic matter, and six perfluoroalkyl acids (PFAAs). Under acidic (pH 4) and neutral (pH 7) conditions, the selectivity of AERs for each contaminant was predominantly influenced by polymer composition followed by the size of the resin functional group. This result reflected the WB-AERs being fully protonated and functioning identical to SB-AERs. Isotherm model parameters revealed WB-AER had higher capacity than SB-AER with analogous polymer composition and porosity regardless of resin selectivity for each contaminant. Under basic conditions (≥ pH 10), contaminant removal by WB-AERs declined due to deprotonation of the tertiary amine functional groups. Removal of PFAAs by the more hydrophobic polystyrene WB-AER (IRA96) remained approximately constant with changing pH, which was possibly due to electrostatic interactions with remaining protonated amine functional groups on the resin.

Amyloid adhesins are β-sheet-rich extracellular proteins thought to contribute to bioflocculation. They are present in activated sludge to varying extent. However, it remains unclear which operational conditions promote their production. To this end, the abundance and distribution of amyloids and their potential producers were monitored in two lab-scale reactors operated in sequencing batch mode with an unaerated and aerated reaction phase. Various feeding regimes ranging from feast-famine to nearly continuous feeding were applied. Thioflavin T staining revealed more amyloids in the lab-scale reactors during all operational stages compared to the full-scale industrial and municipal inocula. Furthermore, the feeding regime impacted the distribution of produced amyloids from dense clusters during feast-famine conditions towards a dispersed distribution during nearly continuous feeding. This dispersed presence did not negatively impact the bioflocculation (towards average floc size and shear sensitivity). 16S rRNA sequencing detected several known EPS and amyloid producers. More continuous and, hence, partially aerobic feeding promoted the relative abundance of denitrifiers. Sequential Thioflavin T staining and fluorescence in situ hybridization identified Zoogloea and Ca. Competibacter as potential amyloid producers under the applied conditions. This experiment confirms that amyloid producers need to be triggered for production and that the feeding regime impacts the microbial community composition, which in turn influences the amyloid production and distribution.

UV-C at 254 nm and vacuum UV (VUV) at 185 nm are the two major emission lines of a low-pressure mercury lamp. Upon absorption of VUV photons, water molecules and selected inorganic anions generate hydroxyl (HO^.) and other redox radicals, both capable of degrading organic micropollutants (OMPs), thereby offering the opportunity to reduce H₂O₂ and energy consumption in UV-based advanced oxidation process (AOP). To be successfully scaled-up, the dual-wavelength VUV+UV/H₂O₂ AOP requires laboratory-scale experiments to establish design criteria. The figures of merit typically used for reporting and interpreting quasi-collimated beam results for UV-based AOPs (time, dose, absorbed energy and E_EO) are insufficient and inaccurate when employed for dual-wavelength AOP such as the VUV+UV/H₂O₂ AOP, and do not support system scale-up. In this study, we introduce a novel figure of merit, useful absorbed energy (uAE), defined as fraction of absorbed energy that results in the generation of oxidative radicals. Here, results of quasi-collimated beam VUV+UV/H₂O₂ AOP experiments on four different water matrices are used to introduce 2D plots that employ both uAE_UV and uAE_VUV as a novel method to represent laboratory-scale experiments of VUV+UV/H₂O₂ AOP and demonstrate how the 2D plots sufficiently support scale-up of the AOP.

Microplastics (MPs) are ubiquitous in the environment and have been found in every environmental compartment. Wastewater and wastewater treatment plants (WWTPs) have been identified as possible point sources contributing to the emission of microplastic particles (MPP) into the aquatic environment. So far, MPP in wastewater effluents have mainly been analyzed by spectroscopic methods resulting in concentrations as number per volume. In this study, we present mass concentrations in the secondary effluents of four German municipal WWTPs, removal efficiencies of seven post-treatment systems and the resulting load emissions. Differential Scanning Calorimetry (DSC) was used for the analysis of semi-crystalline MPs. The concentrations of secondary effluents ranged from 0.1 to 19.6 µg L^-1. Removal efficiencies > 94% were found for a microfiltration membrane (MF), two cloth types of a pile cloth media filter (PCMF), a micro strainer, a discontinuous downflow granulated activated carbon filter (GAC) and a powdered activated carbon (PAC) stage with clarifier and rapid sand filtration. A rapid sand filter (RSF) at WWTP B showed a removal efficiency of 82.38%. Only a continuous upflow GAC filter at WWTP C proved to be unsuitable for MP removal with an average removal efficiency of 1.9%.

Antibiotic resistance is a major 21^st century One Health (humans, animals, environment) challenge whose spread limits options to treat bacterial infections. There is growing interest in monitoring water environments, including surface water and wastewater, which have been identified as key recipients, pathways, and sources of antibiotic resistant bacteria (ARB). Aquatic environments also facilitate the transmission and amplification of ARB. Enterococcus spp. often carry clinically-important antibiotic resistance genes and are of interest as environmental monitoring targets. Enterococcus spp. are Gram-positive bacteria that are typically of fecal origin; however, they are also found in relevant environmental niches, with various species and strains that are opportunistic human pathogens. Although the value of environmental monitoring of antibiotic-resistant Enterococcus has been recognized by both national and international organizations, lack of procedural standardization has hindered generation of comparable data needed to implement integrated surveillance programs. Here we provide a comprehensive methodological review to assess the techniques used for the culturing and characterization of antibiotic-resistant Enterococcus across water matrices for the purpose of environmental monitoring. We analyzed 117 peer-reviewed articles from 33 countries across six continents. The goal of this review is to provide a critical analysis of (i) the various methods applied globally for isolation, confirmation, and speciation of Enterococcus isolates, (ii) the different methods for profiling antibiotic resistance among enterococci, and (iii) the current prevalence of resistance to clinically-relevant antibiotics among Enterococcus spp. isolated from various environments. Finally, we provide advice regarding a path forward for standardizing culturing of Enterococcus spp. for the purpose of antibiotic resistance monitoring in wastewater and wastewater-influenced waters within a global surveillance framework.

Microplastic particles (MP) are efficiently retained in wastewater treatment plants and enriched in sewage sludge. For monitoring MP contents in wastewater systems, sewage sludge is thus well suited, but also requires an isolation of MP from the sludge matrix, as other sewage sludge components may interfere with the MP identification and quantification. Although organic matter in sludge samples can be removed through acid and enzymatic digestion procedures, cellulose - mainly from toilet paper - remains in the digests, due to its high chemical resistivity and similar density to MP. We apply the separation concept of magnetic seeded filtration to isolate MP through selective hetero-agglomeration with magnetic seed particles. MP and cellulose differ in their hydrophobic properties and we investigate to what extent these differences can be exploited to selectively form MP-magnetite hetero-agglomerates in the presence of cellulose. These hetero-agglomerates are subsequently separated using a magnet. Five MP types (Polyethylene terephthalate (PET), polypropylene (PP), low density polyethylene (LDPE), polyvinyl chloride (PVC) and polystyrene (PS)) and cellulose particles were mixed in different combinations with both hydrophilic and hydrophobic (silanized) magnetite particles. PET, PP, LDPE and PS only poorly agglomerated with pristine (hydrophilic) magnetite, but efficiently formed hetero-agglomerates with hydrophobic magnetite and were successfully removed from suspensions ($80-100\%$). PVC agglomerated more efficiently with pristine than with hydrophobic magnetite and cellulose only agglomerated to a limited extent with either hydrophilic or hydrophobic magnetite, resulting in a high process selectivity. Results from experiments conducted at different ionic strengths and with hydrophilic and hydrophobic magnetite suggests that the agglomeration process was dominated by hydrophobic interactions. Enzymatic and oxidative treatment of the MP only marginally affected the separation efficiencies and (treated) MP spiked to sewage sludge extracts were successfully recovered using magnetic seeded filtration.

To ensure access to safe sanitation facilities in rural communities, cheap off-grid technologies need to be developed to substitute pit latrines and open defecation. In this study, we present a prototype system based on hydrothermal oxidation, which, under optimal conditions, converts a fecal sludge simulant almost completely to ${\text{CO}}_2$ and water, leaving behind only a carbon-poor aqueous phase with the minerals. The prototype has been designed to process the feces from two households. This technology does not only enable a fast and complete conversion, but is potentially also very energy efficient, as the feed does not require any pre-treatment or drying. The system was found to effectively remove 97–99% of the total organic carbon within a reaction time of 600 s under an external energy demand of roughly 4 kWh per kilogram of wet feces by using the oxygen in air as an oxidant. A total of ten experiments with varying injection pressure, total solids content of the feed, and residence time in the reactor were performed to find experimental settings with high conversion. Only when the residence time was decreased from 600 to 300 s did the conversion fall significantly below 97%. To reach a target value of 99.9% TOC conversion, the reactor temperature and/or the residence time must be increased further. To achieve a system applicable in regions with no connection to the energy grid, the thermal loss of the reactor insulation needs to be lowered further to achieve an overall thermally self-sustaining operation.