Revista Bionatura最新文献

{"title":"Combination effect of different levels of Nitrogen, Phosphorus and Potassium on the yield of Chickpea Plant (Cicera rietinum L.)","authors":"Yahya A.A Abd Sabri, Alwand T.R. Dizayee","doi":"10.21931/rb/css/2023.08.03.58","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.58","url":null,"abstract":"This experiment was conducted at Grdarasha field of the College of Agricultural Engineering Science, Salahaddin University in Erbil, with a Clay Loam texture class. During the spring growing season of 2020, to study the effect of four levels of phosphorus TSP (0, 20, 40, 60) Kg P. ha-1. Three levels of K (0, 15, 30) Kg K. ha-1 and three levels of N (0, 15, 30) Kg N. ha-1. Besides, there was a combination of yield components and nutrient balance of the chickpea plant using a splitblock design with three factors (K, N and P), where 36 treatment with 3 replicates was used. The main results could be summarized as follows: The combination of potassium, nitrogen, and phosphorus levels affected the yield of the chickpea plant significantly. Also, the maximum yield recorded in the treatment combination (K2N1P2) was (1.55 Mg ha-1). In comparison, the lowest mean value (0.73 Mg ha-1) was recorded from the treatment combination (K0N0P0). Keywords: Nitrogen, Phosphorus, Potassium, chemical and physical properties, chickpea yield","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of COVID-19 infection on the white blood cell count and lymphocyte proliferation activity at the early stage of the disease","authors":"Ali Sadeg, Abbas Arrak","doi":"10.21931/rb/css/2023.08.03.91","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.91","url":null,"abstract":"Since the appearance of COVID-19 at the end of December 2019 in Wuhan, China, and its prevalence in many countries, the symptoms of this disease extended from respiratory problems to a wide range of symptoms associated with the invasion of the virus to many organs and tissues in the body of patients. The white blood cells, particularly T lymphocytes, are the main effectors in defense against viral infections. This study was performed to investigate the response of white blood cells to the infection of SARS-COV-2 at the early stage of the disease. T cells decreased in number in the circulation, but this decrease was not associated with an impairment of their activity. Moreover, stimulation of virus-infected T cells with nonspecific mitogen revealed increased cell proliferation. This study concluded that T lymphocytes are highly activated during SARS-COV-2 infection, despite lymphopenia, at least at the early stages of the disease. Keywords: COVID-19, lymphocyte proliferation, mitotic index","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Artemisia vulgaris L. Aqueous Extract on Some Germination Properties and Seedling Growth of Wheat","authors":"Raghad M. K. Al-Awsi, Dalia S. Hassan","doi":"10.21931/rb/css/2023.08.03.73","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.73","url":null,"abstract":"A laboratory experiment was carried out during the winter season of 2021 in the Seed Technology Laboratory - College of Agricultural Engineering Sciences / University of Baghdad to find out the allopathic effects of aerobic and terrestrial aqueous extracts of artemisia on the seed germination and seedling growth of wheat. A factorial experiment according to a completely randomized design (C.R.D.)at three replicates was used; the first factor included the type of aqueous extract for plant parts, which were aerobic (stems and leaves) and terrestrial (root and rhizomes), while the second factor included five concentrations for each aqueous extract of plant part (0, 25, 50, 75 and 100%). The results showed a significant effect of aqueous extract types on the studied traits; the terrestrial aqueous extract gave the highest germination percentage (70.00%) and radicle dry weight (0.0487 g). Also, the concentration of 50% of artemisia aqueous extract was significantly superior and achieved the highest germination percentage (80.00%) and shoot dry weight (0.0500 g). In contrast, the concentration of 25% was significantly superior in the shoot length (7.47 cm) and radicle dry weight (0.0567 g), while the concentration of 75% was significantly superior in the radicle length (4.17 cm). The interaction between the two factors significantly affected the radicle length and dry weight only. Keywords: Artemisia, weed, allopathic effects, phenolic compound","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135759789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of Enterotoxigenic E. coli and Enteroinvasive E. Coli Among Children with Severe Diarrhea in AL Najaf AL Ashraf / Iraq","authors":"Malak Kahtan Jamal, Ahlam Kadhum Naeem, Hawraa I. Kadhim","doi":"10.21931/rb/css/2023.08.03.76","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.76","url":null,"abstract":"Diarrhea is the second most important disease that causes death in children under 5 years old. This research aimed to investigate the prevalence of Enteroinvasive E. coli (EIEC) and Enterotoxigenic E. coli (ETEC) associated with diarrhea among children under 5 years old. One hundred stool samples have been collected from children under 5 years old suffering from acute diarrhea. All samples are cultured on MacConkey agar and Eosin Methylene Blue (EMB) to detect Diarrheagenic E. coli (DEE). DNA extraction was carried out, and PCR technique. It was used for amplification of the stp gene for the detection of ETEC and the ipaH gene for the detection of EIEC. The results showed a wide distribution of DEC (45%) among males and females, where a high percentage of infection occurred among males (60%) in Compression with females (40%). The Agarose gel electrophoresis results showed that 33% of isolates possessed ipaH, which revealed that these isolates belonged to EIEC. In comparison, 22% of isolates possessed the stp gene, which revealed that these isolates were belong to EHEC. In conclusion, diarrhea may be associated with EIEC and EHEC in addition to EPEC. Keywords: Enterotoxigenic E. coli, Enteroinvasive E. coli, Children with Severe Diarrhea, polymerase chain reaction PCR","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135759795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection of equine infectious anemia viruses using conventional PCR and primer design for virus gag-gene region in the middle Iraqi provinces","authors":"Ahmed Hamzah Mosa, Naseir Mohammed Badawi, Zaid Salah Hussein, Ahmed Jassim Mohammed","doi":"10.21931/rb/css/2023.08.03.62","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.62","url":null,"abstract":"Equine infectious anemia (EIA) is a viral infection that affects Equidae as a persistent infection. Infected horses usually develop a systemic disease with mild clinical signs such as irregular fever, weight loss, anemia, and weakness. The study was the first molecular record of the EIA viruses in horses in Iraq. In total, 72 blood samples (30 horses and 42 donkeys) were selected with history and signs of anemia. The study was conducted in central Iraq (Babylon, Karbala, and Al-Dawinih provinces) from 2015 to 2017. Then, primer design and molecular identification of the gag gene of the EIA virus were performed. The results showed that EIA virus infection rates in horses and donkeys in Iraq's middle regions were 16.66% and 0%, respectively. The DNA sequences of two positive EIA virus samples revealed that isolates have 271 nucleotides under accession numbers MN861108.1 and MN861109.1, respectively. The phylogenetic analysis revealed that Iraqi isolates were clustered in different clades and revealed 83.1– 100% identity with EIAV world isolates in the NCBI. This study was the first molecular record of EIA viruses in Iraq. The study indicated that the gag region was an essential target for EIAV detection. Keywords: PCR, Gag, Gene, EIA, Iraq","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Field survey of fig wax scale insect Ceroplastes rustic L. (Hemiptera: Coccidae) in fig fields of Al-Kifl district/Babylon Governorate and study the effect of some environmental factors on egg hatching","authors":"Muslim Hamza Essa, Khudheyer A.A. Alnomani","doi":"10.21931/rb/css/2023.08.04.100","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.100","url":null,"abstract":"The harmful effects of wax scale insect C.rusci L. on fig trees cultivated widely in the Al-Kifl district / southern Babylon governorate in the last two years was the main reason for designing this study. The study involved experiments; the first was a field survey to have scientific evidence about the widespread of C. rusci and estimated the infection rate in plants, then tried to find a relationship between environmental factors in this area and studied insects. The second experiment deals with the efficacy of some factors (egg locations, temperature and time) on egg hatching of C.rusci. The results provided good information about fig plants' temperature and infection rates with C. rusci. The range of temperature degrees, which showed a high level of infection, was 26.5-33.4 ºC. Meanwhile, the highest infection rate was recorded in October, followed by June. The second experiment showed that temperature was a limiting factor for egg hatching; therefore, there was no hatching of C.rusci eggs when the temperature was below 15 ºC. When the temperature is above this level (15 ºC), a new limitation factor appears in the egg's location. This experiment proved that the existence of an egg under a fold, which covers the insect, is necessary for egg hatching. Keywords: Ceratoplastes rusci, Temperature, Field survey, Egg hatching.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and morphological characterization of the seed gall nematodes Anguina tritici from central and southern Iraq","authors":"Mohammed H. Aldarraji, Dhia S. Alwaily, Ramin Heydari","doi":"10.21931/rb/css/2023.08.04.97","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.97","url":null,"abstract":"This is the first morphological investigation of the wheat gall nematodes Anguina tritici found on wheat in southern and midland areas of Iraq. A survey was conducted, and samples were collected from wheat fields in eight governorates (Diyala, Baghdad, Qadisiyah, Najaf, Wasit, Maysan, Dhi Qar and Basra, 3 regions each and three fields for each region during the 2020-2021 growing season. Wheat-gall nematodes were extracted and observed under a stereomicroscope. Nematodes of interest were hand-picked, identified and photo-micro-graphed using a microscope equipped with a digital camera. Two species of anguinid nematodes (Anguinidae: Tylenchida) were found and characterized. This species is characterized by its 810-905 mm long body, body slightly curved to almost straight, with a 9-10 μm small stylet with delicate lip region very slightly offset, pharyngeal lobe long, wider than adjacent body part and 16-17µm broad, distance of dorsal gland orifice to the style 2-4, position of dorsal gland orifice to the style base expressed as a percentage of the total style length20-40 µm. Anterior end to the valve of the median bulb 67 -72µm; anterior end to excretory. Tail long, gradually tapering to an acute tip without mucro 61 -70 µm. As for the molecular identification and comparison with the GenBank, sequencing of Dhi Qar samples showed having three transitions at sites 59,125,168 GT, GT, and GA, respectively, and one transversion at 140 GC site. It was found that the ITS gene has a compatibility of 98% with the original sequence conserved in the Genbank with ID KC818619. As for the Anguina tritici isolated from Basra, it contains two transitions at locations 247 and 253 CT, four transversions 238,245,249, 251 GT, GC, AC, and TA, respectively, with similarity of 97%. Maysan samples were compatible with 98% and had one transition at 168 GA site and three transversions at the locations 125,128.25 GT. ITS gene from Diyala showed 98% compatibility with the sequence in GenBank, having one transition at locus GA 93 and three transversions at 83,78,59 GT, GC, and GT, respectively. Two transversions in locations 59,117 nucleotide GT, AC in Al-Kut samples, and at 125,142 GT, GC in Baghdad ones, while Najaf samples had one transversion, and one transition in sites 213, 234 GC, CT respectively, as all of them showed 99% similarity with the original sequence in GenBank. Bionatura http://dx.doi.org/10.21931/RB/CSS/2023.08.97 2 Keywords: Field crop, PCR, PPN, wheat","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of various nutmeg concentrations on some common carp growth indicators L. Cyprinus carpio","authors":"Ahmed Hassan Miteib Hulw, Ali Hussain Salman, Jassim Kassim Al-Gharawi","doi":"10.21931/rb/css/2023.08.03.8","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.8","url":null,"abstract":"The study was conducted in special cages in a mud pond, Agricultural Research and Experiment Station, Animal Production Department, Agriculture College, Al-Muthanna University, from 1/10/2020 to 10/12/2021to study the effect of different levels of nutmeg on some growth parameters of common carp. A total of 100 common carp fish, Cyprinus carpio, were brought from a private fish breeding lake in Al-Qadisiyah Governorate, Al-Mihnawiya District, with an average weight of ±.94 gm. 80 fish were selected and distributed randomly and evenly to the experimental cages, put in each aquarium 5 fish for acclimatization, the fish persisted during the acclimatization period, which amounted to 10 days, were starved for one day and then fed with 1% of the weight of the live mass in each tank at the rate of two meals per day. The four treatments were T1 control, T2 0.005%, T3 0.01% and T4 0.015% nutmeg. The results indicated a significant decrease in the final body weight, weight gain, daily growth rate, relative growth rate and specific growth rate in all nutmeg treatments compared to the control treatment. Keywords: Nutmeg, growth parameters, common carp Cyprinus carpio L","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"234 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Examining how PMMA and polyamide denture base materials' physical characteristics are affected by electrolyzed water used as a denture cleaner","authors":"Ahmed Adnan Abed, Aseel Mohammed Al-Khafaji","doi":"10.21931/rb/css/2023.08.03.99","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.99","url":null,"abstract":"Denture cleansing is an essential step that can stop cross‑contamination and adds to the health of the patient, denture durability, and the general quality of life. A disinfection technique must be practical and devoid of damaging effects on the material's properties used to construct the denture base. The main aim of this study is to evaluate the effect of three concentrations of electrolyzed water denture cleanser on heat cure acrylic and polyamide after immersion in electrolyzed water. The evaluation is based on their efficacy on surface hardness, wettability, and color stability compared with one submerged in distilled water as a control group. The method consists of eighty samples of heat-cured acrylic and polyamide material. The samples were immersed in electrolyzed water at a concentration of (100-200ppm) and in distilled water for 5 minutes, 30 times, and daily for 12 days to simulate a one-year interval. The tests showed that the surface hardness and color stability were maintained, with no significant difference between the control and experimental groups. In comparison, the result of wettability showed a statistically significant difference between the control and experimental groups. Thus, electrolyzed water does not affect the surface hardness and color stability of heat-cure acrylic and polyamide denture base materials. However, the wettability of these materials was significantly increased. Keywords: Electrolyzed water; Heat cure acrylic; Polyamide material","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of bacteria from Coptodon zillii (Gervais, 1848) and study of some virulence factors in Al-Diwaniya River/middle of Iraq","authors":"Izdihar Abbas Alwan, Ahmed Kareem Kadhim AL-Wasmee, Mohanad. O. Al-Jubouri","doi":"10.21931/rb/css/2023.08.04.03","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.03","url":null,"abstract":"This study was conceded to examining the occurrence of possible pathogenic species of bacterium in C. zillii in the Al-Diwaniya River. A total of 100 fishes of C. zillii, during the period (April) 2021 till (March) 2022. Bacteria were recognized using the VITEK 2 system and designated biochemical tests. Species were recognized with different percentages in C. zillii, P. luteola, S. thalpophilum, S. lentus, and A. sobria. Antimicrobial susceptibility test of 16 antimicrobials, which are (Ampicillin, piperacillin/tazobactam, amikacin, cefazolin, Ceftriaxone, Ertapenem, Gentamycin, cefoxitin, ciprofloxacin, imipenem, ceftazidime, levofloxacin, cifepime, tigecyclin, Nitrofuran, and trimethoprime- sulphamethoxazole) was recognized and studied for antimicrobial susceptibility test using the Vitek II system. The best antimicrobial susceptibility were ciprofloxacin (<= 0. 11μg/ ml), Imipenem (<= 0. 25, μg/ ml), when tested in vitro on P. luteola, S. thalpophilum, S. lentus, A. sobria, and Livofloxacin (<= 0. 22, <= 0. 20, <= 0. 25, <= 0. 21, μg/ ml), when examined in vitro on P. luteola, S. thalpophilum, S. lentus, A. sobria while, it was resistant to cefazolin ( > = 70, >= 70, > = 76, >= 68, μg/ ml), when examined in vitro on P. luteola, S. thalpophilum, S. lentus, A. sobria. Keywords: Coptodon. zillii bacteria Antibiotics, Iraq.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}