Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.51
Bushra ESAM, RASHA AL SAHLANEE
This study focused on extracting the outer membrane nanovesicles (OMVs) from Escherichia coli BE2 (EC- OMVs) by ultracentrifugation, and the yield was 2.3mg/ml. This was followed by purification with gel filtration chromatography using Sephadex G-150, which was 2mg/ml. The morphology and size of purified EC-OMVs were confirmed by transmission electron microscopy (TEM) at 40-200 nm. The nature of functional groups in the vesicle vesicle was determined by Fourier transforms infrared spectroscopy (FT-IR) analysis. The antitumor activity of EC-OMVs was conducted in vitro by MTT assay in human ovarian (OV33) cancer cell line at 24,48 and 96hrs. The cytotoxicity test showed high susceptibility to the vesicles in ovarian compared to normal cells with IC50 of 7.169 µg/ml at 96hrs of incubation. The current strategy is an effective and simple method to obtain EC-OMVs. Keywords: ovarian cancer, lung cancer, antitumor, breast cancer, nanovesicle, MTT assay.
{"title":"CYTOTOXIC IMPACT OF OUTER MEMBRANE NANOVESICLES (OMVS) ON OVARIAN CANCER","authors":"Bushra ESAM, RASHA AL SAHLANEE","doi":"10.21931/rb/css/2023.08.04.51","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.51","url":null,"abstract":"This study focused on extracting the outer membrane nanovesicles (OMVs) from Escherichia coli BE2 (EC- OMVs) by ultracentrifugation, and the yield was 2.3mg/ml. This was followed by purification with gel filtration chromatography using Sephadex G-150, which was 2mg/ml. The morphology and size of purified EC-OMVs were confirmed by transmission electron microscopy (TEM) at 40-200 nm. The nature of functional groups in the vesicle vesicle was determined by Fourier transforms infrared spectroscopy (FT-IR) analysis. The antitumor activity of EC-OMVs was conducted in vitro by MTT assay in human ovarian (OV33) cancer cell line at 24,48 and 96hrs. The cytotoxicity test showed high susceptibility to the vesicles in ovarian compared to normal cells with IC50 of 7.169 µg/ml at 96hrs of incubation. The current strategy is an effective and simple method to obtain EC-OMVs. Keywords: ovarian cancer, lung cancer, antitumor, breast cancer, nanovesicle, MTT assay.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.82
Anmar A. AlTaie, Muhammad Abdul-Ghani Muhammad, Mohammad M. Salih, Noor Raad Abdulghany, Iman Mutasher Auf
The Bocavirus, which causes respiratory illness in the lower respiratory tract in newborns, children, and healthy adults, was investigated in this study. Methodology and results: The samples were taken as Nasopharyngeal / Throat swabs kept in sterile VTM ( Viral Transport Media ), Which was collected from Mosul Hospitals within six months of the study. The Tested patients' ages ranged from under 20 to above 40 ( between 11 57 ) for both sex males and females. The dominion age group was above 30, and the DNA of this virus was extracted by the real-time PCR method ). Conclusion, significance, and impact of study: the molecular test indicates positive in twenty-four samples from all 70. This demonstrates the presence of Bocavirus in ( 20%) males and ( 14.28%) female understudies. Keywords: Respiratory Tract infections, Bocavirus, Real-time PCR, Virus Transport Media, Viral Transport Media.
{"title":"Human Bocavirus Diagnosis by Molecular Method from Respiratory Infection Patients in Mosul City","authors":"Anmar A. AlTaie, Muhammad Abdul-Ghani Muhammad, Mohammad M. Salih, Noor Raad Abdulghany, Iman Mutasher Auf","doi":"10.21931/rb/css/2023.08.04.82","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.82","url":null,"abstract":"The Bocavirus, which causes respiratory illness in the lower respiratory tract in newborns, children, and healthy adults, was investigated in this study. Methodology and results: The samples were taken as Nasopharyngeal / Throat swabs kept in sterile VTM ( Viral Transport Media ), Which was collected from Mosul Hospitals within six months of the study. The Tested patients' ages ranged from under 20 to above 40 ( between 11 57 ) for both sex males and females. The dominion age group was above 30, and the DNA of this virus was extracted by the real-time PCR method ). Conclusion, significance, and impact of study: the molecular test indicates positive in twenty-four samples from all 70. This demonstrates the presence of Bocavirus in ( 20%) males and ( 14.28%) female understudies. Keywords: Respiratory Tract infections, Bocavirus, Real-time PCR, Virus Transport Media, Viral Transport Media.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.89
Yasmeen N. Tawfeeq, Nihad N. Hilal, Zaidan J. Zaidan
Background: Vitamin B12 is a water-soluble vitamin, and its lack causes the methylation process to be disrupted, further resulting in the formation of abnormal fatty acids, which results in neurological manifestations. The water-soluble vitamin folate, often known as folic acid or vitamin B9, is especially vital during high cell division and growth periods. Objective: The study aims to evaluate serum vitamin B12 and folate levels in type 2 diabetic patients with and without metformin therapy and to correlate their levels with other parameters in type 2 diabetes mellitus (T2DM) patients. Subjects and Methods: A cross-sectional controlled study that included 90 participants, 60 type 2 diabetes mellitus patients with metformin therapy designated as (the metformin group) and 30 type 2 diabetes mellitus without metformin therapy considered as (the control group). The study was conducted from December 2021 to the end of March 2021 at private internal medicine clinics in Tikrit City, Iraq. Blood samples were assessed for serum vitamin B12 and folate using Cl-900i Chemiluminescence Immunoassay Analyzer and glycated hemoglobin HbA1c using AFIAS Analyzer in all metformin and control groups. Result: The study showed that there were significant differences in vitamin B12 of (p < 0.01) and folate of (p < 0.05) between the metformin group and the control group. Significant negative correlations were found between vitamin B12 and diabetes mellitus (DM) duration and HbA1c. Folate was negatively correlated and highly significant with DM duration and HbA1c (p < 0.01). Conclusion: The results showed low vitamin B12 and folate levels in T2DM patients. Vitamin B12 and folate were negatively correlated with DM duration and HbA1c. The results suggest a higher risk of vitamin B12 and folate deficiency with metformin use and emphasize the concern of vitamin B12 and folate deficiency in T2DM patients. Keywords: Type 2 Diabetes Mellitus (T2DM); Vitamin B12; Folate; DM duration; HbA1c.
{"title":"Association between Serum Vitamin B12 and Folate Levels with Type 2 Diabetes Mellitus On Metformin Therapy","authors":"Yasmeen N. Tawfeeq, Nihad N. Hilal, Zaidan J. Zaidan","doi":"10.21931/rb/css/2023.08.04.89","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.89","url":null,"abstract":"Background: Vitamin B12 is a water-soluble vitamin, and its lack causes the methylation process to be disrupted, further resulting in the formation of abnormal fatty acids, which results in neurological manifestations. The water-soluble vitamin folate, often known as folic acid or vitamin B9, is especially vital during high cell division and growth periods. Objective: The study aims to evaluate serum vitamin B12 and folate levels in type 2 diabetic patients with and without metformin therapy and to correlate their levels with other parameters in type 2 diabetes mellitus (T2DM) patients. Subjects and Methods: A cross-sectional controlled study that included 90 participants, 60 type 2 diabetes mellitus patients with metformin therapy designated as (the metformin group) and 30 type 2 diabetes mellitus without metformin therapy considered as (the control group). The study was conducted from December 2021 to the end of March 2021 at private internal medicine clinics in Tikrit City, Iraq. Blood samples were assessed for serum vitamin B12 and folate using Cl-900i Chemiluminescence Immunoassay Analyzer and glycated hemoglobin HbA1c using AFIAS Analyzer in all metformin and control groups. Result: The study showed that there were significant differences in vitamin B12 of (p < 0.01) and folate of (p < 0.05) between the metformin group and the control group. Significant negative correlations were found between vitamin B12 and diabetes mellitus (DM) duration and HbA1c. Folate was negatively correlated and highly significant with DM duration and HbA1c (p < 0.01). Conclusion: The results showed low vitamin B12 and folate levels in T2DM patients. Vitamin B12 and folate were negatively correlated with DM duration and HbA1c. The results suggest a higher risk of vitamin B12 and folate deficiency with metformin use and emphasize the concern of vitamin B12 and folate deficiency in T2DM patients. Keywords: Type 2 Diabetes Mellitus (T2DM); Vitamin B12; Folate; DM duration; HbA1c.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.53
Firas Ibrahim Rahim, Mohmmed K. Abdullah, Najlaa S. Madlul
The importance of the cucumber crop is highlighted because it is important from a nutritional and health point of view for humans because of its importance in the economy. A random sample was selected for this research estimated at.(75) farmers from the producers of the cucumber crop in Salah al-Din Governorate - Tikrit district. Moreover, the Data was taken from farmers using a questionnaire form. Information about The merchants was taken from the wholesale market in the Tikrit district using (34) A questionnaire, as well as collecting (46) From the retailers' forms. The results showed that the commission rate increased to (5%) of the product prices, and this is the reason for the high price of the product among retailers. The share of the product from consumer spending for the cucumber crop has reached (49.46%). As for the wholesaler's share of consumer spending for the crop under study (18.53%). As for the retailer's share of consumer spending for the crop under study (32%). As for the mediators’ share of the consumer’s dinar for the crop under study (50.53%). The results showed that the marketing margin between the wholesalers' stage and the product was (27.25%) dinars/kg. In comparison, the relative margin for the wholesalers and retailers stage amounted to (32%) and the margin between the retailers stage and product (50(. The study showed a rise in the profits of the retailer from the margin, as it ranked first, as it reached an average of the cucumber crop of about (218.44 dinars - kg), while the profit for the wholesaler amounted to about (132.57 dinars/ kg The product profits amounted to about (98.655) dinars - kg. The researcher recommended working to provide production requirements and agricultural marketing requirements for the cucumber crop with good quality and appropriate prices, which have an effective role in reducing production and marketing costs. Key Words: The Economics, Marketing, Cucumber Crop.
{"title":"The Economics of Marketing the Cucumber Crop in Salah al-Din Governorate, Tikrit district, as a model for The Summer Production Season 2021","authors":"Firas Ibrahim Rahim, Mohmmed K. Abdullah, Najlaa S. Madlul","doi":"10.21931/rb/css/2023.08.04.53","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.53","url":null,"abstract":"The importance of the cucumber crop is highlighted because it is important from a nutritional and health point of view for humans because of its importance in the economy. A random sample was selected for this research estimated at.(75) farmers from the producers of the cucumber crop in Salah al-Din Governorate - Tikrit district. Moreover, the Data was taken from farmers using a questionnaire form. Information about The merchants was taken from the wholesale market in the Tikrit district using (34) A questionnaire, as well as collecting (46) From the retailers' forms. The results showed that the commission rate increased to (5%) of the product prices, and this is the reason for the high price of the product among retailers. The share of the product from consumer spending for the cucumber crop has reached (49.46%). As for the wholesaler's share of consumer spending for the crop under study (18.53%). As for the retailer's share of consumer spending for the crop under study (32%). As for the mediators’ share of the consumer’s dinar for the crop under study (50.53%). The results showed that the marketing margin between the wholesalers' stage and the product was (27.25%) dinars/kg. In comparison, the relative margin for the wholesalers and retailers stage amounted to (32%) and the margin between the retailers stage and product (50(. The study showed a rise in the profits of the retailer from the margin, as it ranked first, as it reached an average of the cucumber crop of about (218.44 dinars - kg), while the profit for the wholesaler amounted to about (132.57 dinars/ kg The product profits amounted to about (98.655) dinars - kg. The researcher recommended working to provide production requirements and agricultural marketing requirements for the cucumber crop with good quality and appropriate prices, which have an effective role in reducing production and marketing costs. Key Words: The Economics, Marketing, Cucumber Crop.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.80
Mona Najm Khalaf, Alaa Ayed Obaid
The current study aimed to use three cultivars of Iraqi dates in the date stage, AlZuhdi hard, Sayer semi-hard and soft Paraben) and fumigating these dates with ethyl formate to preserve them and studying the physical traits of the dates under study in three periods (the pre-storage period, the three-month post-storage period and the six-month storage period).The physical traits included (total soluble solids, pH, viscosity, relative viscosity, surface tension, density, refractive index, and electrical conductivity. Keywords: Dates Dusting; Ethyl Formate; Al-Zuhdi; Paraben; Sayer.
{"title":"Studying the effect of dusting with ethyl formate on the physical traits of some Iraqi date cultivars","authors":"Mona Najm Khalaf, Alaa Ayed Obaid","doi":"10.21931/rb/css/2023.08.04.80","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.80","url":null,"abstract":"The current study aimed to use three cultivars of Iraqi dates in the date stage, AlZuhdi hard, Sayer semi-hard and soft Paraben) and fumigating these dates with ethyl formate to preserve them and studying the physical traits of the dates under study in three periods (the pre-storage period, the three-month post-storage period and the six-month storage period).The physical traits included (total soluble solids, pH, viscosity, relative viscosity, surface tension, density, refractive index, and electrical conductivity. Keywords: Dates Dusting; Ethyl Formate; Al-Zuhdi; Paraben; Sayer.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.67
Mohamed Mahmoud Shiaa, Firas Ibrahim Arhim
The research aims to estimate the technical and specialized efficiency and the economic efficiency of wheat crop varieties according to the data envelope analysis method (DEA) and according to the varieties in the research sample, which are (Bura, Adana, Iba 99), and the data was obtained by designing a questionnaire using the random sampling method for farms in the field. It determined the size of the samples for the studied items according to their percentage in the community for (150) farms by (50 for each item in the research sample. The results of the efficiency analysis were obtained according to the data envelope for production functions and costs with stability and change in capacity returns. The results of the analysis showed, depending on the variables of the production function, The results showed that the farms that achieved full efficiency (100%) were (11) farms, which were divided into (3) farms for the (Bura) variety, (6) farms for the (Adena) variety, and (2) farms for the (Ibaa 99) variety. These farms can be counted. References to the sample farms that did not reach full efficiency and the number of farms that achieved 100% efficiency in light of the change in yield were (18) farms, divided (6) farms for the (Bura) variety, (8) farms for the (Adena) and (4) farms for the variety (Ibaa 99), respectively, while the number of farms that did not reach 100% efficiency in light of the change in the field Lead (44, 42, 46) farms for varieties (Bura, Adana, Ibaa 99), respectively. Depending on the variables of the cost function, it turns out that the total of the farms that achieved 100% allocative efficiency (6) farms with one farm for the Pura variety (4) farms for the variety below and one farm for the Ibaa 99 variety. In this case, these farms do not have any surplus inputs because they consume all inputs. The optimum size to reach the optimum production and the results of estimating the economic efficiency of the wheat crop and the studied varieties were also shown, as the average economic efficiency was (0.51, 0.44, 0.41) for varieties (Bura, Aden, Iba 99), respectively. This level is low, which is the result of the product of each From technical efficiency and allocative efficiency, which shows that these farms can reduce costs by (49%, 56%, and 59%) and achieve the same production level or that these farms can get the current production using (51%, 44%, 41%) of resources to become economically efficient. In light of the results that have been reached, the research recommended encouraging farmers to use the seeds of modern varieties with high production to make the best Bionatura http://dx.doi.org/10.21931/RB/CSS/2023.08.04.67 2 use of agricultural areas in order to give the best production in addition to benefiting from the efficiency indicators obtained through the data envelope model, and the need to activate the role of the agricultural extension system in alerting farmers to the use of modern agricultural methods in line wit
{"title":"Estimation of technical efficiency levels using the Data Envelope method (DEA) for wheat crop cultivars farms in Diyala Governorate for the production season (2020-2021)","authors":"Mohamed Mahmoud Shiaa, Firas Ibrahim Arhim","doi":"10.21931/rb/css/2023.08.04.67","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.67","url":null,"abstract":"The research aims to estimate the technical and specialized efficiency and the economic efficiency of wheat crop varieties according to the data envelope analysis method (DEA) and according to the varieties in the research sample, which are (Bura, Adana, Iba 99), and the data was obtained by designing a questionnaire using the random sampling method for farms in the field. It determined the size of the samples for the studied items according to their percentage in the community for (150) farms by (50 for each item in the research sample. The results of the efficiency analysis were obtained according to the data envelope for production functions and costs with stability and change in capacity returns. The results of the analysis showed, depending on the variables of the production function, The results showed that the farms that achieved full efficiency (100%) were (11) farms, which were divided into (3) farms for the (Bura) variety, (6) farms for the (Adena) variety, and (2) farms for the (Ibaa 99) variety. These farms can be counted. References to the sample farms that did not reach full efficiency and the number of farms that achieved 100% efficiency in light of the change in yield were (18) farms, divided (6) farms for the (Bura) variety, (8) farms for the (Adena) and (4) farms for the variety (Ibaa 99), respectively, while the number of farms that did not reach 100% efficiency in light of the change in the field Lead (44, 42, 46) farms for varieties (Bura, Adana, Ibaa 99), respectively. Depending on the variables of the cost function, it turns out that the total of the farms that achieved 100% allocative efficiency (6) farms with one farm for the Pura variety (4) farms for the variety below and one farm for the Ibaa 99 variety. In this case, these farms do not have any surplus inputs because they consume all inputs. The optimum size to reach the optimum production and the results of estimating the economic efficiency of the wheat crop and the studied varieties were also shown, as the average economic efficiency was (0.51, 0.44, 0.41) for varieties (Bura, Aden, Iba 99), respectively. This level is low, which is the result of the product of each From technical efficiency and allocative efficiency, which shows that these farms can reduce costs by (49%, 56%, and 59%) and achieve the same production level or that these farms can get the current production using (51%, 44%, 41%) of resources to become economically efficient. In light of the results that have been reached, the research recommended encouraging farmers to use the seeds of modern varieties with high production to make the best Bionatura http://dx.doi.org/10.21931/RB/CSS/2023.08.04.67 2 use of agricultural areas in order to give the best production in addition to benefiting from the efficiency indicators obtained through the data envelope model, and the need to activate the role of the agricultural extension system in alerting farmers to the use of modern agricultural methods in line wit","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.47
Walaa zayer Shnain, Hasanain N. Ezzat
This study was conducted in the poultry field of the Department of Animal Production / College of Agricultural Engineering Sciences / University of Baghdad Abu Ghraib for the period from 10/15/2021 to 11/25/2021 to show the effect of adding different levels of dill seeds to the diet on productive and carcass traits For broiler meat. In this study, 200 unsexed broiler chicks of breed (308 Ross) were used, one day age, with a starting weight of (42) g. The chicks were randomly distributed to 5 treatments, and each treatment included 4 replicates, 10 birds for each replicate. The birds were fed three diets: the starter diet, the growth diet and the final diet. The experiment treatments were T1, T2, T3, T4 and T5, adding dill seeds at average (0, 0.3, 0.6, 0.9, 1.2%), respectively. The experiment results indicated a significantly excelled(P < 0.05) for treatment T5. Compared with the T1 treatment, it did not differ significantly from the rest of the treatments; adding dill seeds T2, T3 and T4 in body weight, weight gain and feed consumed for broilers at 42 days, an improvement was observed in the food conversion ratio of treatment T5 compared to the rest of the treatments in the first week, and in the fourth week, the results indicated a significant improvement (0.01>P) in the feed conversion ratio of the addition treatments T2, T3, T4 and T5 compared to treatment T1. It was found that there was a significant (P < 0.05) excel in the carcass weight of the T5 treatment compared to the T4 treatment. It did not differ significantly with the T1, T2 and T3 treatments, and a significant (P < 0.05) in the relative weight of the gizzard for the T4 treatment compared to the T1 treatment and the liver relative weight for the two treatments. T4 and T5 as compared to T1. Also, a significant excel (P<0.05) was observed in the relative weight of the thigh for treatment T1 and T4 compared to T5. When calculating the relative weight of the neck, it was noted that treatment T1 compared to treatment T5. When calculating the relative weight of the back, treatment T4 excelled on the rest of the treatments. We conclude from this study that adding 1.2% of dill seeds to the diet improved the productive traits of broilers. Keywords: dill seeds, broilers, productive performance, carcasses.
{"title":"Effect of adding different levels of dill seeds to the diet on productive traits and some carcass traits of broiler chickens","authors":"Walaa zayer Shnain, Hasanain N. Ezzat","doi":"10.21931/rb/css/2023.08.03.47","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.47","url":null,"abstract":"This study was conducted in the poultry field of the Department of Animal Production / College of Agricultural Engineering Sciences / University of Baghdad\u0000Abu Ghraib for the period from 10/15/2021 to 11/25/2021 to show the effect of\u0000adding different levels of dill seeds to the diet on productive and carcass traits For\u0000broiler meat. In this study, 200 unsexed broiler chicks of breed (308 Ross) were\u0000used, one day age, with a starting weight of (42) g. The chicks were randomly\u0000distributed to 5 treatments, and each treatment included 4 replicates, 10 birds for\u0000each replicate. The birds were fed three diets: the starter diet, the growth diet and\u0000the final diet. The experiment treatments were T1, T2, T3, T4 and T5, adding dill\u0000seeds at average (0, 0.3, 0.6, 0.9, 1.2%), respectively. The experiment results indicated a significantly excelled(P < 0.05) for treatment T5. Compared with the T1\u0000treatment, it did not differ significantly from the rest of the treatments; adding dill\u0000seeds T2, T3 and T4 in body weight, weight gain and feed consumed for broilers\u0000at 42 days, an improvement was observed in the food conversion ratio of treatment\u0000T5 compared to the rest of the treatments in the first week, and in the fourth week,\u0000the results indicated a significant improvement (0.01>P) in the feed conversion\u0000ratio of the addition treatments T2, T3, T4 and T5 compared to treatment T1. It\u0000was found that there was a significant (P < 0.05) excel in the carcass weight of the\u0000T5 treatment compared to the T4 treatment. It did not differ significantly with the\u0000T1, T2 and T3 treatments, and a significant (P < 0.05) in the relative weight of the\u0000gizzard for the T4 treatment compared to the T1 treatment and the liver relative\u0000weight for the two treatments. T4 and T5 as compared to T1. Also, a significant\u0000excel (P<0.05) was observed in the relative weight of the thigh for treatment T1\u0000and T4 compared to T5. When calculating the relative weight of the neck, it was\u0000noted that treatment T1 compared to treatment T5. When calculating the relative\u0000weight of the back, treatment T4 excelled on the rest of the treatments. We conclude from this study that adding 1.2% of dill seeds to the diet improved the productive traits of broilers.\u0000Keywords: dill seeds, broilers, productive performance, carcasses.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"2011 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.86
Mohammed S . Mahmud, Lamia S. Ashoor
Amis: NAFLD is considered to be the most common cause of liver conditions worldwide. Also, it is a primary reason that leads to coronary artery diseases, limiting blood flow to the heart. Therefore, This study aimed to evaluate the serum level of Nesfatin-1 and its ability to indicate the prognosis of CAD in patients with NAFLD. Material & Methods: one-hundred eighty Individuals were enrolled in the study, including In both genders, blood was collected from each Individual and sent to the laboratory for biochemical tests. Findings: Data from the current study showed a significant increase in Nesfatin-1 in the CAD group and a significant decrease in Nesfatin-1 in the NAFLD group compared to the control group. In addition, there was also a significant increase in both cardiac parameters and AST in the CAD group compared to the NAFLD group and the control group. Conclusion: Patients with coronary artery disease have higher Nesfatin-1 Concentration due to Nesfatin-1 having anti-inflammatory properties that raise the level of Nesfatin-1. In addition, Data from the current study showed a significant positive correlation between Nesfatin-1 and (ALT and AST) in NAFLD patients. However, further studies are needed to confirm this conclusion. Keywords: Coronary Artery Disease, Non-alcoholic fatty liver disease, Nesfatin1, Troponin-I
{"title":"Nesfatin-1 is a biomarker that plays a role in the inflammatory process of coronary artery diseases in Iraqi patients with non-alcoholic fatty liver disease.","authors":"Mohammed S . Mahmud, Lamia S. Ashoor","doi":"10.21931/rb/css/2023.08.03.86","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.86","url":null,"abstract":"Amis: NAFLD is considered to be the most common cause of liver conditions worldwide. Also, it is a primary reason that leads to coronary artery diseases, limiting blood flow to the heart. Therefore, This study aimed to evaluate the serum level of Nesfatin-1 and its ability to indicate the prognosis of CAD in patients with NAFLD. Material & Methods: one-hundred eighty Individuals were enrolled in the study, including In both genders, blood was collected from each Individual and sent to the laboratory for biochemical tests. Findings: Data from the current study showed a significant increase in Nesfatin-1 in the CAD group and a significant decrease in Nesfatin-1 in the NAFLD group compared to the control group. In addition, there was also a significant increase in both cardiac parameters and AST in the CAD group compared to the NAFLD group and the control group. Conclusion: Patients with coronary artery disease have higher Nesfatin-1 Concentration due to Nesfatin-1 having anti-inflammatory properties that raise the level of Nesfatin-1. In addition, Data from the current study showed a significant positive correlation between Nesfatin-1 and (ALT and AST) in NAFLD patients. However, further studies are needed to confirm this conclusion. Keywords: Coronary Artery Disease, Non-alcoholic fatty liver disease, Nesfatin1, Troponin-I","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.66
Raghda Hatem Omran, Zahra’a Abdul-Raheem Ahmed
The present study aimed to evaluate the serum level of interleukins 32 and 37 (IL-32 and IL-37) in rheumatoid arthritis (RA) patients. An ELISA was used to measure the levels of cytokines in the blood of 56 RA patients and 44 healthy volunteers who were enrolled in the study from November 2021 to March 2022. Serum levels of IL-32 and IL-37 in the RA patients were significantly higher compared to the control groups (IL32, p = 0.035; IL37, p = 0.011). In addition, cytokine concentration levels were higher in RA patients under therapy than in RA patients with a first diagnosis and without therapy, with significant differences in IL-37(141.389 ± 24.133 vs. 58.852 ± 7.806 ng/L). In comparison, there were no significant differences in IL-32 (93.740 ± 10.835 vs. 76.722 ± 9.321 ng/L). The results suggest that pro-inflammatory and anti-inflammatory cytokines may play an essential role in the progression of RA. Keywords: Autoimmunity, IL-32, IL-37, Rheumatoid arthritis.
本研究旨在评价类风湿关节炎(RA)患者血清白细胞介素32和37 (IL-32和IL-37)水平。在2021年11月至2022年3月期间，研究人员使用ELISA来测量56名RA患者和44名健康志愿者血液中的细胞因子水平。RA患者血清IL-32、IL-37水平显著高于对照组(IL-32, p = 0.035;IL37, p = 0.011)。此外，接受治疗的RA患者的细胞因子浓度水平高于首次诊断和未接受治疗的RA患者，IL-37的差异具有统计学意义(141.389±24.133比58.852±7.806 ng/L)。相比之下，IL-32(93.740±10.835 vs. 76.722±9.321 ng/L)差异无统计学意义。结果提示，促炎和抗炎细胞因子可能在RA的进展中起重要作用。关键词:自身免疫，IL-32, IL-37，类风湿关节炎
{"title":"Evaluation of IL-32 and IL-37 levels in Rheumatoid Arthritis","authors":"Raghda Hatem Omran, Zahra’a Abdul-Raheem Ahmed","doi":"10.21931/rb/css/2023.08.03.66","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.66","url":null,"abstract":"The present study aimed to evaluate the serum level of interleukins 32 and 37 (IL-32 and IL-37) in rheumatoid arthritis (RA) patients. An ELISA was used to measure the levels of cytokines in the blood of 56 RA patients and 44 healthy volunteers who were enrolled in the study from November 2021 to March 2022. Serum levels of IL-32 and IL-37 in the RA patients were significantly higher compared to the control groups (IL32, p = 0.035; IL37, p = 0.011). In addition, cytokine concentration levels were higher in RA patients under therapy than in RA patients with a first diagnosis and without therapy, with significant differences in IL-37(141.389 ± 24.133 vs. 58.852 ± 7.806 ng/L). In comparison, there were no significant differences in IL-32 (93.740 ± 10.835 vs. 76.722 ± 9.321 ng/L). The results suggest that pro-inflammatory and anti-inflammatory cytokines may play an essential role in the progression of RA. Keywords: Autoimmunity, IL-32, IL-37, Rheumatoid arthritis.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135761489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.20
Three genotypes of maize, namely (Baghdad, 5018, and Sara) were investigated in the fields of the College of Agricultural Engineering Sciences, University of Baghdad/ Al-Jadriya, in the fall season 2021-2022. With three sowing dates (26 July, 4 August, 12 August), The experiment was implemented according to a randomized complete block design (RCBD) with three replications. The aim is to detect the expression of the INCW1 gene associated with sucrose-to-glucose analysis, which is critical for many metabolic functions in different sink tissues, and determine the amount of fold gene expression to find out which of the genotypes contain the invertase gene, and which ones have a high level of gene expression based on the fold of gene expression. The results showed significant differences in the invertase enzyme activity for the genotypes. The best genotype was (Sara) which showed a value of 1.14 mg-1.units.protein—followed genotype (Baghdad) 0.86 mg-1.units.protein. The least effective protein was for genotype (5018), which showed a value of 0.40 mg-1.units.protein. As for the sowing dates, the second sowing date (D2) was the best, which showed a value of 1.39 mg-1.units.protein. Followed by the third sowing date (D3), which showed a value of 0.51 mg-1.units.protein, and the first sowing date (D1), which showed the most negligible value of 0.51 mg-1units. The expression of the INCW1 gene varied in the genotypes of three sowing dates with values ranging between (0.30) and (2.38), where the genotype (Sara) at the second sowing date (D2), which showed the value highest of gene expression (2.38) and the exact date (D3) gave INCW1 gene showed value (2.21) for genotype (Baghdad). The genotypes also varied in their tolerance to increase or decrease the gene expression, which is considered one of the most important sucrose decomposition genes for maize crops. Keywords: - Invertase, gene expression, Zea mays. L, Sowing date, Genotype.
{"title":"Gene expression in some maize genotypes","authors":"","doi":"10.21931/rb/css/2023.08.04.20","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.20","url":null,"abstract":"Three genotypes of maize, namely (Baghdad, 5018, and Sara) were investigated in\u0000the fields of the College of Agricultural Engineering Sciences, University of\u0000Baghdad/ Al-Jadriya, in the fall season 2021-2022. With three sowing dates (26\u0000July, 4 August, 12 August), The experiment was implemented according to a\u0000randomized complete block design (RCBD) with three replications. The aim is to\u0000detect the expression of the INCW1 gene associated with sucrose-to-glucose\u0000analysis, which is critical for many metabolic functions in different sink tissues,\u0000and determine the amount of fold gene expression to find out which of the genotypes contain the invertase gene, and which ones have a high level of gene expression based on the fold of gene expression. The results showed significant\u0000differences in the invertase enzyme activity for the genotypes. The best genotype\u0000was (Sara) which showed a value of 1.14 mg-1.units.protein—followed genotype\u0000(Baghdad) 0.86 mg-1.units.protein. The least effective protein was for genotype\u0000(5018), which showed a value of 0.40 mg-1.units.protein. As for the sowing dates,\u0000the second sowing date (D2) was the best, which showed a value of 1.39\u0000mg-1.units.protein. Followed by the third sowing date (D3), which showed a value\u0000of 0.51 mg-1.units.protein, and the first sowing date (D1), which showed the most\u0000negligible value of 0.51 mg-1units. The expression of the INCW1 gene varied in\u0000the genotypes of three sowing dates with values ranging between (0.30) and (2.38),\u0000where the genotype (Sara) at the second sowing date (D2), which showed the value\u0000highest of gene expression (2.38) and the exact date (D3) gave INCW1 gene\u0000showed value (2.21) for genotype (Baghdad). The genotypes also varied in their\u0000tolerance to increase or decrease the gene expression, which is considered one of\u0000the most important sucrose decomposition genes for maize crops.\u0000Keywords: - Invertase, gene expression, Zea mays. L, Sowing date, Genotype.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"280 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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