Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.69
Saif Abdulameer Salman, Ziena M.Abdul-Qader
The experiment was carried out in one of the fields of Research Station B in the College of Agricultural Engineering Sciences / University of Baghdad - Al-Jadiriya on the fragrant plant for the spring season 2021 in order to study the effect of harvest date, mycorrhiza, bio-stimulants and the interaction among them on some characteristics of vegetative growth and the essential oil yield of sweet-scented geranium plants. The experiment used a randomized complete block design (RCBD) with a split-plot design with three replications. The experiment included three factors, as the factor of harvest dates represents the main panels with two harvest dates, which are 60 and 90 after planting and symbolized by H1 and H2. In contrast, the secondary panels include the treatment of the mycorrhizal fungal vaccine by two treatments, namely, not adding the mycorrhizal fungal vaccine to the root system and adding it with symbols M1 and M2 As for the sub-secondary panels, the treatments include four stimulating factors: the measurement treatment and the spraying of the vegetative mass with the amino acid phenylalanine at a concentration of 300 mg l ̄¹. The treatment of spraying the foliage with moringa leaf extract at a concentration of 10 gm L ̄¹ and the treatment of spraying the foliage with licorice root powder extract at a concentration of 10gm L ̄¹symbolized by B1, B2, B3, and B4 respectively. The results showed the superiority of the H2 treatment in most indicators of vegetative growth, as well as the increase in the percentages of each of the nutrients and volatile oil in the dried leaves and the volatile oil yield in the dried leaves. Treatment M2 significantly increased all vegetative growth characteristics as well as the percentages of nutrients and volatile oil in dried leaves and volatile oil yield in dried leaves. In contrast, treatment B3 showed an increase in the number of main branches, total number of leaves, total leaf area, fresh and dry weight of leaf yield in the plant and the content of The dried leaves of total chlorophyll as well as the percentages of nutrients and volatile oil in the dried leaves of the plant and the yield of volatile oil in the dried leaves. In contrast, treatment B2 had a significant effect in increasing the plant height rate, the number of main branches and the fresh weight of the leaves. It is one of the most prominent triple interaction treatments recorded. The largest increase in all the traits studied above is the triple interaction H2M2B3 treatment, which was characterized by an Bionatura http://dx.doi.org/10.21931/RB/CSS/2023.08.04.69 2 increase in most vegetative growth characteristics, especially the wet and dry weight of leaves and the percentages of nutrients as well as the percentage of volatile oil and volatile oil yield in the leaves. The triple interaction treatment H2M2B4 was also characterized by its recording of Remarkable superiority in both plant height and leaf content of Total chlorophyll and potass
为了研究采收日期、菌根、生物刺激剂及其相互作用对甜天竺葵植物营养生长和精油产量的影响,本试验于2021年春季在巴格达大学农业工程科学学院B研究站进行。试验采用随机完全区组设计(RCBD),分图设计,3个重复。试验包括三个因素,收获日期因素代表主面板上有两个收获日期,分别是种植后的60和90,用H1和H2表示。次级板包括菌根真菌疫苗不向根系添加和标记M1、M2两种处理,次次级板包括4种刺激因素:计量处理和300 mg l * 1的苯丙氨酸氨基酸喷施。喷施浓度为10gm L ā 1的辣木叶提取物处理和喷施浓度为10gm L ā 1的甘草根粉提取物处理,分别用B1、B2、B3和B4表示。结果表明,H2处理在大多数营养生长指标上都具有优势,且干叶中各营养成分和挥发油含量的百分比以及干叶挥发油产量均有提高。M2处理显著提高了干叶营养物质含量和挥发油含量,显著提高了干叶挥发油产量。相比之下,B3处理增加了植株主枝数、总叶数、总叶面积、鲜重和干重、叶片产量、总叶绿素含量、干叶中营养物质和挥发油的百分比以及干叶中挥发油的产量。B2处理在提高株高率、主枝数和叶片鲜重方面效果显著。这是记录中最突出的三重相互作用治疗之一。在上述各项性状中增幅最大的是H2M2B3三重互作处理,其特点是在大多数营养生长性状中,尤其是叶片的干、湿重、养分含量百分比、叶片挥发油含量百分比和挥发油产量方面均有一个Bionatura http://dx.doi.org/10.21931/RB/CSS/2023.08.04.69 2的提高。三重互作处理H2M2B4在株高、叶片总叶绿素含量和钾含量方面均有显著优势。关键词:天竺葵,采收日期,菌根,生物刺激素,营养生长,挥发油
{"title":"Effect of harvest dates, mycorrhiza, and some biostimulants on some vegetative growth properties and yield of volatile oil for sweet scented geranium plant (Pelargonium graveolens L.Herit.)","authors":"Saif Abdulameer Salman, Ziena M.Abdul-Qader","doi":"10.21931/rb/css/2023.08.04.69","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.69","url":null,"abstract":"The experiment was carried out in one of the fields of Research Station B in the College of Agricultural Engineering Sciences / University of Baghdad - Al-Jadiriya on the fragrant plant for the spring season 2021 in order to study the effect of harvest date, mycorrhiza, bio-stimulants and the interaction among them on some characteristics of vegetative growth and the essential oil yield of sweet-scented geranium plants. The experiment used a randomized complete block design (RCBD) with a split-plot design with three replications. The experiment included three factors, as the factor of harvest dates represents the main panels with two harvest dates, which are 60 and 90 after planting and symbolized by H1 and H2. In contrast, the secondary panels include the treatment of the mycorrhizal fungal vaccine by two treatments, namely, not adding the mycorrhizal fungal vaccine to the root system and adding it with symbols M1 and M2 As for the sub-secondary panels, the treatments include four stimulating factors: the measurement treatment and the spraying of the vegetative mass with the amino acid phenylalanine at a concentration of 300 mg l ̄¹. The treatment of spraying the foliage with moringa leaf extract at a concentration of 10 gm L ̄¹ and the treatment of spraying the foliage with licorice root powder extract at a concentration of 10gm L ̄¹symbolized by B1, B2, B3, and B4 respectively. The results showed the superiority of the H2 treatment in most indicators of vegetative growth, as well as the increase in the percentages of each of the nutrients and volatile oil in the dried leaves and the volatile oil yield in the dried leaves. Treatment M2 significantly increased all vegetative growth characteristics as well as the percentages of nutrients and volatile oil in dried leaves and volatile oil yield in dried leaves. In contrast, treatment B3 showed an increase in the number of main branches, total number of leaves, total leaf area, fresh and dry weight of leaf yield in the plant and the content of The dried leaves of total chlorophyll as well as the percentages of nutrients and volatile oil in the dried leaves of the plant and the yield of volatile oil in the dried leaves. In contrast, treatment B2 had a significant effect in increasing the plant height rate, the number of main branches and the fresh weight of the leaves. It is one of the most prominent triple interaction treatments recorded. The largest increase in all the traits studied above is the triple interaction H2M2B3 treatment, which was characterized by an Bionatura http://dx.doi.org/10.21931/RB/CSS/2023.08.04.69 2 increase in most vegetative growth characteristics, especially the wet and dry weight of leaves and the percentages of nutrients as well as the percentage of volatile oil and volatile oil yield in the leaves. The triple interaction treatment H2M2B4 was also characterized by its recording of Remarkable superiority in both plant height and leaf content of Total chlorophyll and potass","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.53
Sally K. Abd. Alaameri, Huda S. A. Al-Hayanni, Labeeb A. K. Al-Zubaidi
Multidrug-resistant bacteria have contributed to a rise in morbidity and death from microbial infections, making it more difficult to treat illnesses caused by resistant pathogenic bacteria. There is a growing interest in using nanoparticles as biomaterials around the globe. Nanoparticles might become a crucial, feasible therapeutic alternative for treating infections resistant to several drugs. Currently, they are recognized as feasible alternatives or additions to conventional antimicrobials. Nanotechnology focuses on developing eco-friendly methods for producing nanoparticles. The current work aims to generate a quick, ecologically friendly approach for synthesizing silver nanoparticles utilizing aqueous and alcohol sumac plant extracts. These silver nanoparticles appear synthesized and capped by sumac extract bioactive components. FTIR, XRD, SEM, AAS, EDX, AFM, and UV-Vis spectroscopy were used to determine the nanoparticle structure, shape, and optical properties. Within 15 minutes, the AgNPs had formed. The aqueous and alcoholic sumac extracts resulted in silver nanoparticles with an average particle size of 43.82 nm and 39.55 nm, respectively. Against the multi-drug-resistant clinical isolates, silver nanoparticles from both extracts had good antibacterial activity (Staphylococcus aureus, Acinetobacter baumannii, Enterococcus faecalis, and Pseudomonas aeruginosa). Also, these nanoparticles had an inhibitory effect on the productivity of the biofilm virulence factor. For this study with highly significant differences (P≤0.01). Keywords: Silver nanoparticles, Green synthesis, Rhus coriaria L., Sumac, antibacterial activity, antibiofilm activity; multidrug-resistant bacteria.
{"title":"Antibacterial and anti-biofilm properties of biosynthesized Silver nanoparticles using Sumac (Rhus coriaria L.) extracts against some pathogenic bacteria","authors":"Sally K. Abd. Alaameri, Huda S. A. Al-Hayanni, Labeeb A. K. Al-Zubaidi","doi":"10.21931/rb/css/2023.08.03.53","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.53","url":null,"abstract":"Multidrug-resistant bacteria have contributed to a rise in morbidity and death from microbial infections, making it more difficult to treat illnesses caused by resistant pathogenic bacteria. There is a growing interest in using nanoparticles as biomaterials around the globe. Nanoparticles might become a crucial, feasible therapeutic alternative for treating infections resistant to several drugs. Currently, they are recognized as feasible alternatives or additions to conventional antimicrobials. Nanotechnology focuses on developing eco-friendly methods for producing nanoparticles. The current work aims to generate a quick, ecologically friendly approach for synthesizing silver nanoparticles utilizing aqueous and alcohol sumac plant extracts. These silver nanoparticles appear synthesized and capped by sumac extract bioactive components. FTIR, XRD, SEM, AAS, EDX, AFM, and UV-Vis spectroscopy were used to determine the nanoparticle structure, shape, and optical properties. Within 15 minutes, the AgNPs had formed. The aqueous and alcoholic sumac extracts resulted in silver nanoparticles with an average particle size of 43.82 nm and 39.55 nm, respectively. Against the multi-drug-resistant clinical isolates, silver nanoparticles from both extracts had good antibacterial activity (Staphylococcus aureus, Acinetobacter baumannii, Enterococcus faecalis, and Pseudomonas aeruginosa). Also, these nanoparticles had an inhibitory effect on the productivity of the biofilm virulence factor. For this study with highly significant differences (P≤0.01). Keywords: Silver nanoparticles, Green synthesis, Rhus coriaria L., Sumac, antibacterial activity, antibiofilm activity; multidrug-resistant bacteria.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136183698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.29
Abeer Mounir Abdel Hadi, Bushra Mahmoud Alwan
An experiment was carried out in one of the fields of the Soil Research Department - Agricultural Research Department/Ministry of Agriculture in the Abu Ghraib district at latitude 33° 17" 31 N and longitude 44° 03" 56 E and 35 m above sea level. This field study was conducted in the autumn season 2021-2022 for the cultivation of yellow maize ( Maha cultivar) in sedimentary soil with silty clay loam texture, with a Split Plot Design. The treatments were distributed using the RCBD for three replicates with two factors. The first factor represents the main plots that include the addition of mineral fertilizer 120, 80, and 320 kg.ha-1 for each of (N, P, and K), respectively, at three levels (0, 50, and 100%) of the fertilizer recommendation. On the other hand, the second factor was a subplot of organic fertilizer (palm frond compost) at three levels (0, 5, and 10) μg.ha-1 . Soil samples were taken to estimate the availability of nitrogen, phosphorous, and potassium, where the total yield and percentage of protein for yellow maize were estimated. The results showed that adding organic fertilizers (palm fronds) with the levels of mineral fertilizers increased the availability of nutrients and the yield of maize. Accordingly, the treatment O2M2 achieved the highest values of nutrient availability, quantity, and quality of yield, reaching (59.167, 33.407, and 257.967) mg.kg-1 soil and (10,096) μg.ha-1 and (9.435%) for each of nitrogen, phosphorous, potassium, yield, and protein percentage, respectively. Moreover, there were no significant differences between the treatments of O1M2 and O2M1 in the amount of yield and the percentage of protein, which gave the values of 8.403 and 8.134 μg.ha-1, respectively, and the protein percentage of 8.630 and 8.915% respectively. It can be concluded that organic fertilizers can be added to compensate for mineral fertilizers, reduce environmental pollution, and reduce the economic cost and soil preservation because organic fertilizers are considered safe and environmentally friendly. Keywords: yellow maize, mineral fertilizer, palm fronds compost.
{"title":"Effect of Adding Levels of Palm Fronds Compost and Mineral Fertilizer on Fertile Soil Characteristics, Quality, and Productivity of Maize Yield (Zea Mays L.)","authors":"Abeer Mounir Abdel Hadi, Bushra Mahmoud Alwan","doi":"10.21931/rb/css/2023.08.03.29","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.29","url":null,"abstract":"An experiment was carried out in one of the fields of the Soil Research Department - Agricultural Research Department/Ministry of Agriculture in the Abu Ghraib district at latitude 33° 17\" 31 N and longitude 44° 03\" 56 E and 35 m above sea level. This field study was conducted in the autumn season 2021-2022 for the cultivation of yellow maize ( Maha cultivar) in sedimentary soil with silty clay loam texture, with a Split Plot Design. The treatments were distributed using the RCBD for three replicates with two factors. The first factor represents the main plots that include the addition of mineral fertilizer 120, 80, and 320 kg.ha-1 for each of (N, P, and K), respectively, at three levels (0, 50, and 100%) of the fertilizer recommendation. On the other hand, the second factor was a subplot of organic fertilizer (palm frond compost) at three levels (0, 5, and 10) μg.ha-1 . Soil samples were taken to estimate the availability of nitrogen, phosphorous, and potassium, where the total yield and percentage of protein for yellow maize were estimated. The results showed that adding organic fertilizers (palm fronds) with the levels of mineral fertilizers increased the availability of nutrients and the yield of maize. Accordingly, the treatment O2M2 achieved the highest values of nutrient availability, quantity, and quality of yield, reaching (59.167, 33.407, and 257.967) mg.kg-1 soil and (10,096) μg.ha-1 and (9.435%) for each of nitrogen, phosphorous, potassium, yield, and protein percentage, respectively. Moreover, there were no significant differences between the treatments of O1M2 and O2M1 in the amount of yield and the percentage of protein, which gave the values of 8.403 and 8.134 μg.ha-1, respectively, and the protein percentage of 8.630 and 8.915% respectively. It can be concluded that organic fertilizers can be added to compensate for mineral fertilizers, reduce environmental pollution, and reduce the economic cost and soil preservation because organic fertilizers are considered safe and environmentally friendly. Keywords: yellow maize, mineral fertilizer, palm fronds compost.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.86
Najwa Imad Sulaiman Saleh, Siham Agmee Wadee, Entedhar R. Sarhat
Doxorubicin (DOX) is a highly effective drug for chemotherapy. However, hepatotoxicity reduces its clinical utility in humans. Thus, this study was designed to examine Date Palm extract on serum anti-inflammatory markers (interleukin(IL) IL-1B, IL-6 and IL-10). Forty adult rats were divided into 4 groups (G1 control, G2 receiving 2mgkg of DPE orally, G3 treated with 2mgkg of DOX IP, and G4 received 2mgkg of DOX via IP and 2mgkg of DPE by oral gavage daily for 30 days). At the end of the study, animals were sacrificed, and livers were analyzed histologically. The Dox group showed significantly higher levels of serum IL-1B, IL-6, and IL-10 than the control group, with inflammation and necrosis in hepatic histopathology. In the DPE+ DOX group, it was detected that DOX treatment caused a significant decrease in serum IL-1B, IL-6, and IL-10 levels. Collectively, pre-coadministration of DPE partially mitigated DOX-induced hepatic injuries via its antioxidant, anti-inflammatory, anti-fibrotic, and antiapoptotic protein.
阿霉素(DOX)是一种非常有效的化疗药物。然而,肝毒性降低了其在人类中的临床应用。因此,本研究旨在检测枣椰树提取物对血清抗炎标志物(白细胞介素(IL) IL- 1b、IL-6和IL-10)的影响。将40只成年大鼠分为4组(G1对照组,G2组口服DPE 2mgkg, G3组口服DOX IP 2mgkg, G4组每日口服DOX IP 2mgkg和DPE 2mgkg,连续30 d)。研究结束时,处死动物,对肝脏进行组织学分析。Dox组大鼠血清IL-1B、IL-6、IL-10水平明显高于对照组,肝脏组织病理表现为炎症、坏死。在DPE+ DOX组中,检测到DOX治疗导致血清IL-1B、IL-6和IL-10水平显著降低。总的来说,预共给药DPE通过其抗氧化、抗炎、抗纤维化和抗凋亡蛋白部分减轻了dox诱导的肝损伤。
{"title":"Minimizing the side effects of Doxorubicin Induced Hepatotoxicity by using alcoholic extract of Date Palm in adult rats","authors":"Najwa Imad Sulaiman Saleh, Siham Agmee Wadee, Entedhar R. Sarhat","doi":"10.21931/rb/css/2023.08.04.86","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.86","url":null,"abstract":"Doxorubicin (DOX) is a highly effective drug for chemotherapy. However, hepatotoxicity reduces its clinical utility in humans. Thus, this study was designed to examine Date Palm extract on serum anti-inflammatory markers (interleukin(IL) IL-1B, IL-6 and IL-10). Forty adult rats were divided into 4 groups (G1 control, G2 receiving 2mgkg of DPE orally, G3 treated with 2mgkg of DOX IP, and G4 received 2mgkg of DOX via IP and 2mgkg of DPE by oral gavage daily for 30 days). At the end of the study, animals were sacrificed, and livers were analyzed histologically. The Dox group showed significantly higher levels of serum IL-1B, IL-6, and IL-10 than the control group, with inflammation and necrosis in hepatic histopathology. In the DPE+ DOX group, it was detected that DOX treatment caused a significant decrease in serum IL-1B, IL-6, and IL-10 levels. Collectively, pre-coadministration of DPE partially mitigated DOX-induced hepatic injuries via its antioxidant, anti-inflammatory, anti-fibrotic, and antiapoptotic protein.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.88
Ali Sami Ahmed Alhayali, Waseem Ali Hasan, Firas Subhi Salah
The combination of anti-cancer drugs improves effectiveness compared to the mono-therapy scenario by targeting key pathways synergistically or in an additive way. Doxazosin (DOX) and Resveratrol (RES) are reported to have an anti-cancer impact against different cancer cells. Aim: To evaluate the anti-cancer properties of Doxazosin and Resveratrol, each alone or in combination, in inhibiting breast cancer cell proliferation. Methods: MCF-7 cancer cells were seeded to a confluent monolayer and treated with 100, 50, 25, 12.5, 6.25, and 3.12 µM of each drug alone and as a combination. Cytotoxicity was evaluated using dimethyl thiazolyl diphenyl tetrazolium salt (MTT) assay and colony formation assay. The immunocytochemistry technique was conducted to evaluate caspase-3 and GABARAP expression. Results: All the drugs have a significant effect on cancer cell proliferation. The combination treatment of Doxazosin-Resveratrol has a more cytotoxic effect than each drug alone, as well as in colony formation of MCF-7 cancer cells. In combination treatment, caspase-3 had a higher expression pattern than other treatments. Resveratrol treatment elevated GABARAP expression in cancer cells, indicating the induction of the autophagy process. Conclusion: Although each drug has its characteristic result of a cytotoxic effect, the combination of Doxazosin and Resveratrol synergizes the inhibition of MCF-7 cancer cell proliferation. Keywords: Resveratrol; Doxazosin; combination; MCF-7; Autophagy.
{"title":"Autophagy induction using Resveratrol enhances the anti-cancer efficacy of Doxazosin in breast cancer cells.","authors":"Ali Sami Ahmed Alhayali, Waseem Ali Hasan, Firas Subhi Salah","doi":"10.21931/rb/css/2023.08.04.88","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.88","url":null,"abstract":"The combination of anti-cancer drugs improves effectiveness compared to the mono-therapy scenario by targeting key pathways synergistically or in an additive way. Doxazosin (DOX) and Resveratrol (RES) are reported to have an anti-cancer impact against different cancer cells. Aim: To evaluate the anti-cancer properties of Doxazosin and Resveratrol, each alone or in combination, in inhibiting breast cancer cell proliferation. Methods: MCF-7 cancer cells were seeded to a confluent monolayer and treated with 100, 50, 25, 12.5, 6.25, and 3.12 µM of each drug alone and as a combination. Cytotoxicity was evaluated using dimethyl thiazolyl diphenyl tetrazolium salt (MTT) assay and colony formation assay. The immunocytochemistry technique was conducted to evaluate caspase-3 and GABARAP expression. Results: All the drugs have a significant effect on cancer cell proliferation. The combination treatment of Doxazosin-Resveratrol has a more cytotoxic effect than each drug alone, as well as in colony formation of MCF-7 cancer cells. In combination treatment, caspase-3 had a higher expression pattern than other treatments. Resveratrol treatment elevated GABARAP expression in cancer cells, indicating the induction of the autophagy process. Conclusion: Although each drug has its characteristic result of a cytotoxic effect, the combination of Doxazosin and Resveratrol synergizes the inhibition of MCF-7 cancer cell proliferation. Keywords: Resveratrol; Doxazosin; combination; MCF-7; Autophagy.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.70
Sally S. Mohammed AL-jubouri, AbdulMuhsin Moslim Shami
Escherichia coli are a normal flora in the human. It is pathogenic in patients with immune system disorders and is the leading cause of enteritis, urinary tract infection, septicemia and other medical infections. E. coli is the most common cause of community and hospital-acquired urinary tract infections (UTIs). It is responsible for >80% of all cases of UTI. These study samples were taken from Al-Karama Teaching Hospital and Al-Yarmouk Teaching Hospital in Baghdad. This study aimed to determine the Minimum Inhibition concentration (MIC) of Ceftazidim. As well as comparing the effect of antibiotics and without antibiotics on beta-lactam genes (OXA-48 –CTXM-1) resistance to Cephalosporin where there is an overexpression of gene expression in the antibiotic between (125-64) µg/ml. Bacteria produce beta-lactam enzymes that break down the beta-lactam ring in the antibiotic, which reduces the effectiveness of antibiotics. This mechanism is one of the mechanisms of resistance in bacteria. The presence of both genes increases the resistance of this species to Cephalosporin. The results of gene expression when treated with antibiotics for the OXA-48 gene in his sample were 1.4, and the highest value was 14.5, as well as for the CTX-M-1 gene, where the results ranged between the lowest value of 1.3 and the highest value of 7.3 when compared to the control samples, we notice an overexpression of gene. It was concluded that the resistance of E. coli to Ceftazidim was related to the genes blaOXA45 and CTXM-1, but the primary role may be due to blaOXA45. Keywords: Gene Expression, OXA-48 Gene, CTX-M-1 Gene, Cephalosporin, Escherichia coli.
{"title":"Study Gene Expression of OXA-48and CTX-M-1 Genes Cephalosporin resistance in Escherichia coli isolated from urinary tract infections in Baghdad hospitals","authors":"Sally S. Mohammed AL-jubouri, AbdulMuhsin Moslim Shami","doi":"10.21931/rb/css/2023.08.03.70","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.70","url":null,"abstract":"Escherichia coli are a normal flora in the human. It is pathogenic in patients with immune system disorders and is the leading cause of enteritis, urinary tract infection, septicemia and other medical infections. E. coli is the most common cause of community and hospital-acquired urinary tract infections (UTIs). It is responsible for >80% of all cases of UTI. These study samples were taken from Al-Karama Teaching Hospital and Al-Yarmouk Teaching Hospital in Baghdad. This study aimed to determine the Minimum Inhibition concentration (MIC) of Ceftazidim. As well as comparing the effect of antibiotics and without antibiotics on beta-lactam genes (OXA-48 –CTXM-1) resistance to Cephalosporin where there is an overexpression of gene expression in the antibiotic between (125-64) µg/ml. Bacteria produce beta-lactam enzymes that break down the beta-lactam ring in the antibiotic, which reduces the effectiveness of antibiotics. This mechanism is one of the mechanisms of resistance in bacteria. The presence of both genes increases the resistance of this species to Cephalosporin. The results of gene expression when treated with antibiotics for the OXA-48 gene in his sample were 1.4, and the highest value was 14.5, as well as for the CTX-M-1 gene, where the results ranged between the lowest value of 1.3 and the highest value of 7.3 when compared to the control samples, we notice an overexpression of gene. It was concluded that the resistance of E. coli to Ceftazidim was related to the genes blaOXA45 and CTXM-1, but the primary role may be due to blaOXA45. Keywords: Gene Expression, OXA-48 Gene, CTX-M-1 Gene, Cephalosporin, Escherichia coli.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135759791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.60
Sahar Hamdan Alani, Luma Musa Ibrahim, Hala A. Alwan, Modar Abdel-Abbas
Background: The mental foramen is a strategically important landmark during osteotomy procedures. Its location and the possibility that an anterior loop of the mental nerve may be present mesial to the mental foramen needs to be considered before implant surgery to avoid mental nerve injury. Material and method: This is a retrospective cross-sectional study conducted using panoramic radiographic data of Iraqi regular healthy patients visiting dental teaching Hospitals at Al Farahidi College of Dentistry .The number of participants selected for this study is 40, 20 male and 20 female. Results: A total of 45 were processed and analyzed during the periods of the current study. Out of these, only 40 were considered sufficient for the inclusion and exclusion criteria. Fifty Bee Gees for five subjects were discarded because they were not matched with the inclusion and exclusion criteria. The remaining 40 Bee Gees represent 20 male and 20 female subjects. All the data were matched to get a better picture and comprehensive results. Keywords: Mental Foramen, dental implant, mental foramen
{"title":"Relation of Mental Foramen to dental implant","authors":"Sahar Hamdan Alani, Luma Musa Ibrahim, Hala A. Alwan, Modar Abdel-Abbas","doi":"10.21931/rb/css/2023.08.03.60","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.60","url":null,"abstract":"Background: The mental foramen is a strategically important landmark during osteotomy procedures. Its location and the possibility that an anterior loop of the mental nerve may be present mesial to the mental foramen needs to be considered before implant surgery to avoid mental nerve injury. Material and method: This is a retrospective cross-sectional study conducted using panoramic radiographic data of Iraqi regular healthy patients visiting dental teaching Hospitals at Al Farahidi College of Dentistry .The number of participants selected for this study is 40, 20 male and 20 female. Results: A total of 45 were processed and analyzed during the periods of the current study. Out of these, only 40 were considered sufficient for the inclusion and exclusion criteria. Fifty Bee Gees for five subjects were discarded because they were not matched with the inclusion and exclusion criteria. The remaining 40 Bee Gees represent 20 male and 20 female subjects. All the data were matched to get a better picture and comprehensive results. Keywords: Mental Foramen, dental implant, mental foramen","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.03.63
Khalid Hadi Kadhim, Diyar Mohammad Hussein, Shaima Khazaal Waad
This study aimed to describe some morphological and histological aspects of the binni fish's gills. Fifteen adult male binni were pulled alive from the Al-Forat River at about (24 - 28) months, immediately after death. The gills of binni were situated on each side of the head beneath a gill cover; the operculum and the gills were made of finger-like long filaments joined to the cartilaginous gill bar. From each filament, numerous fragile lamellae that resemble leaves protrude. These lamellae comprised tiny capillaries covered in plain squamous epithelial cells. The epithelium acts as a barrier between the fish's blood and the surrounding water. The first, second, third, and fourth pairs of gill arches make up the gills supported by the bone skeleton. Each arch carries a row of gill filaments on its convex side and two rows of gill rakers on its concave side. On the rostral concave border, the filaments in the two extremes of the gill were short, but the length of all the filaments was the same. The first-gill arch's lateral rakers were longer than the medial rakers, while the fourth gill arch's medial rakers were longer than the lateral rakers. The rakers, bony comb-like projections, filled the rostral region of the gill arches at the rostral concave interior side. There was interdigitation between the gill rakers of the neighboring gill arches. Each gill arch included the lateral gill rakers pointing dorsolaterally and medial gill rakers pointing dorsomedially. Filaments originate from the gill arches and are maintained by the cartilage on the caudal convex border of the primary lamellae, from which the secondary lamellae emerge. The simple squamous epithelium lined the secondary lamellae. Keywords: Binni, Gill, Morphology, Histology
{"title":"Some morphological structures and histological features of the gills of Binni (Mesopotamichthys sharpeyi)","authors":"Khalid Hadi Kadhim, Diyar Mohammad Hussein, Shaima Khazaal Waad","doi":"10.21931/rb/css/2023.08.03.63","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.03.63","url":null,"abstract":"This study aimed to describe some morphological and histological aspects of the binni fish's gills. Fifteen adult male binni were pulled alive from the Al-Forat River at about (24 - 28) months, immediately after death. The gills of binni were situated on each side of the head beneath a gill cover; the operculum and the gills were made of finger-like long filaments joined to the cartilaginous gill bar. From each filament, numerous fragile lamellae that resemble leaves protrude. These lamellae comprised tiny capillaries covered in plain squamous epithelial cells. The epithelium acts as a barrier between the fish's blood and the surrounding water. The first, second, third, and fourth pairs of gill arches make up the gills supported by the bone skeleton. Each arch carries a row of gill filaments on its convex side and two rows of gill rakers on its concave side. On the rostral concave border, the filaments in the two extremes of the gill were short, but the length of all the filaments was the same. The first-gill arch's lateral rakers were longer than the medial rakers, while the fourth gill arch's medial rakers were longer than the lateral rakers. The rakers, bony comb-like projections, filled the rostral region of the gill arches at the rostral concave interior side. There was interdigitation between the gill rakers of the neighboring gill arches. Each gill arch included the lateral gill rakers pointing dorsolaterally and medial gill rakers pointing dorsomedially. Filaments originate from the gill arches and are maintained by the cartilage on the caudal convex border of the primary lamellae, from which the secondary lamellae emerge. The simple squamous epithelium lined the secondary lamellae. Keywords: Binni, Gill, Morphology, Histology","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.60
Emad Khalaf Khedher Al-qassi, Mohammed Ibrahim Mohammed
An experiment was conducted to study five hybrids, four of them introduced (Zwin, Zp, Syngenta, and Kws) and the local hybrid Baghdad in the spring lug and with three planting dates (5/3, 15/3, and 25/3) and in the two seasons 2019-2020 and 2020-2021 in the design of randomized complete sectors to study the performance of the Genotype stability in three planting dates and two seasons. Thus, we have six environments, Where the highest genetic variation coefficient in the first and second environments was in the number of grains per ear, which amounted to 11.84 and 12.37, respectively. In the third environment, the weight of the ear reached 25.08, and in the fourth, fifth and sixth environment, the number of grains per ear reached 15.14, 24.93 and 13.39, respectively. The results were different genotypes and genetic parameters in their performance in different environments. The two genotypes (Zwin and Kws) showed significance in the highest number of traits, in contrast to the rest. The superiority of the Syngenta genotype was shown in leaf area, leaf area index, plant height, ear diameter, number of rows per ear, the weight of 300 grains, grain yield, and protein percentage, which were 4027, 3.29, 162.8, 51.03, 17.16, 60.09, 3.99, and 11.22 on the respectively, then the Zp genotype in tasselling and silking, ear length and number of grains per ear, as their average values were 54.12, 59.09, 17.92 and 466.8, respectively. Keywords: Maize; Stability; Environmental; Genetic Parameter; Stability Triangle
{"title":"Performance Evaluation and Estimation of Stability Parameters of Maize Genotypes Zea mays L. in Different Environments","authors":"Emad Khalaf Khedher Al-qassi, Mohammed Ibrahim Mohammed","doi":"10.21931/rb/css/2023.08.04.60","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.60","url":null,"abstract":"An experiment was conducted to study five hybrids, four of them introduced\u0000(Zwin, Zp, Syngenta, and Kws) and the local hybrid Baghdad in the spring lug and\u0000with three planting dates (5/3, 15/3, and 25/3) and in the two seasons 2019-2020\u0000and 2020-2021 in the design of randomized complete sectors to study the performance of the Genotype stability in three planting dates and two seasons. Thus, we\u0000have six environments, Where the highest genetic variation coefficient in the first\u0000and second environments was in the number of grains per ear, which amounted to\u000011.84 and 12.37, respectively. In the third environment, the weight of the ear\u0000reached 25.08, and in the fourth, fifth and sixth environment, the number of grains\u0000per ear reached 15.14, 24.93 and 13.39, respectively. The results were different\u0000genotypes and genetic parameters in their performance in different environments.\u0000The two genotypes (Zwin and Kws) showed significance in the highest number of\u0000traits, in contrast to the rest. The superiority of the Syngenta genotype was shown\u0000in leaf area, leaf area index, plant height, ear diameter, number of rows per ear, the\u0000weight of 300 grains, grain yield, and protein percentage, which were 4027, 3.29,\u0000162.8, 51.03, 17.16, 60.09, 3.99, and 11.22 on the respectively, then the Zp genotype in tasselling and silking, ear length and number of grains per ear, as their\u0000average values were 54.12, 59.09, 17.92 and 466.8, respectively.\u0000Keywords: Maize; Stability; Environmental; Genetic Parameter; Stability Triangle","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-15DOI: 10.21931/rb/css/2023.08.04.81
Al-Hassan Jamal Abdul Razzaq, Shaker and Iman Qassem Mohammed AlBahrani
To know the effect of bio-enhancer (zeolite), biohealth, mineral fertilizers and their interactions, the possibility of replacing mineral fertilizers with bio-enhancers and bio-enhancers, and their effect on some potato yield measurements. A field experiment was conducted at one of the field stations of the College of Agricultural Engineering Sciences, University of Baghdad, near the electronic calculator center, research station (F) in Al-Jadriya region in the loam mixture soil during autumn season 2021-2022 AD, It was designed using a completely randomized block design (RCBD) with three replicates. The factors of the study experiment included three levels of zeolite (0, 6 tons ha-1, and 12 tons ha-1), which were symbolized by (Z0), (Z1) and (Z2), respectively. As for the bio-enhancer (Biohealth), it was added at two levels (0 and 5) to kg ha-1, which was symbolized by the symbols (B0) and (B1), respectively. As for the mineral fertilizer treatments, they were added at three levels (0, 50%, and 75%) of the fertilizer recommendation, which was (300 kg ha-1 N, 100 kg ha-1 P, and 300 kg ha-1 potassium), symbolized by (F0). and (F1) and (F2), respectively. Potato seed, Rivera cultivar, was planted as a furrow on 1/23/2022. The area of the experimental unit was 6 m 2 (3 m 2 x 2 m2 ). Eighteen treatments were distributed randomly in the sectors (replicates), so the number of units became 54 experimental units. Keywords: Potato; Biohealth; Zeolite; Mineral Fertilizer.
了解生物增强剂(沸石)、生物保健剂、矿物肥料的作用及其相互作用,生物增强剂和生物增强剂替代矿物肥料的可能性,以及它们对某些马铃薯产量测量的影响。采用完全随机区组设计(RCBD), 3个重复,于2021-2022年秋季在巴格达大学农业工程科学学院位于Al-Jadriya地区电子计算器中心附近的试验站(F)进行田间试验。研究实验的影响因素包括沸石的3个水平(0、6吨ha-1和12吨ha-1),分别用(Z0)、(Z1)和(Z2)表示。对于生物增强剂(Biohealth),在kg ha-1的基础上分别添加0和5两个水平,分别用符号(B0)和(B1)表示。矿物肥处理按推荐施肥量的0、50%、75% (300 kg hm -1 N、100 kg hm -1 P、300 kg hm -1钾)3个水平添加,用(F0)表示。分别为(F1)和(F2)。土豆种子,里维拉品种,于2022年1月23日作为犁沟种植。实验单元面积为6 m2 (3 m2 × 2 m2)。18个处理随机分布在扇区(重复),试验单元数为54个。关键词:马铃薯;Biohealth;沸石;矿物肥料。
{"title":"Effect of adding Biohealth, zeolite and mineral fertilizer on some measurements of potato yield","authors":"Al-Hassan Jamal Abdul Razzaq, Shaker and Iman Qassem Mohammed AlBahrani","doi":"10.21931/rb/css/2023.08.04.81","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.04.81","url":null,"abstract":"To know the effect of bio-enhancer (zeolite), biohealth, mineral fertilizers and their interactions, the possibility of replacing mineral fertilizers with bio-enhancers and bio-enhancers, and their effect on some potato yield measurements. A field experiment was conducted at one of the field stations of the College of Agricultural Engineering Sciences, University of Baghdad, near the electronic calculator center, research station (F) in Al-Jadriya region in the loam mixture soil during autumn season 2021-2022 AD, It was designed using a completely randomized block design (RCBD) with three replicates. The factors of the study experiment included three levels of zeolite (0, 6 tons ha-1, and 12 tons ha-1), which were symbolized by (Z0), (Z1) and (Z2), respectively. As for the bio-enhancer (Biohealth), it was added at two levels (0 and 5) to kg ha-1, which was symbolized by the symbols (B0) and (B1), respectively. As for the mineral fertilizer treatments, they were added at three levels (0, 50%, and 75%) of the fertilizer recommendation, which was (300 kg ha-1 N, 100 kg ha-1 P, and 300 kg ha-1 potassium), symbolized by (F0). and (F1) and (F2), respectively. Potato seed, Rivera cultivar, was planted as a furrow on 1/23/2022. The area of the experimental unit was 6 m 2 (3 m 2 x 2 m2 ). Eighteen treatments were distributed randomly in the sectors (replicates), so the number of units became 54 experimental units. Keywords: Potato; Biohealth; Zeolite; Mineral Fertilizer.","PeriodicalId":52287,"journal":{"name":"Revista Bionatura","volume":"172 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135760667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}