محمد امینی, ایرج خدادادی, حیدر طیبینیا, سید حسن طالبی, بهروز افشار, جمشید صدر کریمی
{"title":"افزایش بیان ژنهای TPT1-AS1 و SAMMSON و کاهش بیان ژن LNC00961 به عنوان نشانگرهای بالقوه تومور در سرطان معده","authors":"محمد امینی, ایرج خدادادی, حیدر طیبینیا, سید حسن طالبی, بهروز افشار, جمشید صدر کریمی","doi":"10.52547/AJCM.27.4.201","DOIUrl":"https://doi.org/10.52547/AJCM.27.4.201","url":null,"abstract":"","PeriodicalId":52678,"journal":{"name":"pzshkhy blyny bn syn","volume":"27 1","pages":"201-210"},"PeriodicalIF":0.0,"publicationDate":"2021-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70683260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Bazzazi, Mona Sharafi Zamir, S. Akbarzadeh, Y. Mohammadi, M. Ahmadpanah
Background and Objective: Diabetic retinopathy is one of the most important and debilitating complications of diabetes with an increasing prevalence in most countries. It has a dramatic effect on people's quality of life and increases the symptoms of anxiety and depression in patients with diabetes. Therefore, the present study aimed to determine the prevalence of depression in patients with diabetic retinopathy. Materials and Methods: This descriptive/ cross-sectional study was conducted on 245 patients with retinopathy referred to the ophthalmology clinic of Farshchian Hospital in Hamadan during 20192020. They were selected via available and consecutive sampling method and were evaluated for depression with the 21-item Beck questionnaire. Data were analyzed in SPSS software (version 16) at a 95% confidence level. Results: The mean scores of patients' age and duration of diabetes were reported as 54.47± 8.5 and 8.5 ± 4.15 years, respectively. In terms of gender, the majority of participants (58.7%) were female. The prevalence of depression in patients with diabetic retinopathy was obtained at 49.8% and in proliferative and non-proliferative retinopathy were 68.2% and 43%, respectively (P<0.001). The prevalence rates of depression in men and women were reported as 45.9% and 51.5% (P=0.428). Moreover, in patients treated with insulin and without insulin, the prevalence rates of depression were obtained at 50.4% and 49.2% (P=0.846). The correlation coefficients of depressive disorder with age and duration of diabetes were calculated at 0.123 (P=0.054) and 0.369 (P<0.001), respectively. Conclusion: Depression is one of the most serious problems in patients with diabetic retinopathy. As evidenced by the results of the present study, the prevalence of depression is significantly correlated with the severity of retinopathy and duration of diabetes.
{"title":"Prevalence of Depression in Patients with Diabetic Retinopathy","authors":"N. Bazzazi, Mona Sharafi Zamir, S. Akbarzadeh, Y. Mohammadi, M. Ahmadpanah","doi":"10.52547/ajcm.27.4.226","DOIUrl":"https://doi.org/10.52547/ajcm.27.4.226","url":null,"abstract":"Background and Objective: Diabetic retinopathy is one of the most important and debilitating complications of diabetes with an increasing prevalence in most countries. It has a dramatic effect on people's quality of life and increases the symptoms of anxiety and depression in patients with diabetes. Therefore, the present study aimed to determine the prevalence of depression in patients with diabetic retinopathy. Materials and Methods: This descriptive/ cross-sectional study was conducted on 245 patients with retinopathy referred to the ophthalmology clinic of Farshchian Hospital in Hamadan during 20192020. They were selected via available and consecutive sampling method and were evaluated for depression with the 21-item Beck questionnaire. Data were analyzed in SPSS software (version 16) at a 95% confidence level. Results: The mean scores of patients' age and duration of diabetes were reported as 54.47± 8.5 and 8.5 ± 4.15 years, respectively. In terms of gender, the majority of participants (58.7%) were female. The prevalence of depression in patients with diabetic retinopathy was obtained at 49.8% and in proliferative and non-proliferative retinopathy were 68.2% and 43%, respectively (P<0.001). The prevalence rates of depression in men and women were reported as 45.9% and 51.5% (P=0.428). Moreover, in patients treated with insulin and without insulin, the prevalence rates of depression were obtained at 50.4% and 49.2% (P=0.846). The correlation coefficients of depressive disorder with age and duration of diabetes were calculated at 0.123 (P=0.054) and 0.369 (P<0.001), respectively. Conclusion: Depression is one of the most serious problems in patients with diabetic retinopathy. As evidenced by the results of the present study, the prevalence of depression is significantly correlated with the severity of retinopathy and duration of diabetes.","PeriodicalId":52678,"journal":{"name":"pzshkhy blyny bn syn","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46090667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Nevus sebaceous is a cutaneous hamartoma accompanied by secondary tumors, such as syringocystadenoma papilliferum. Syringocystadenoma papilliferum is a rare benign tumor with a controversial differentiation between eccrine and apocrine. Most syringocystadenoma papilliferum involving the scalp arises from a nevus sebaceous. Case Presentation: Herein, we report a 15-year-old adolescent female with a congenital skin lesion on the forehead undergoing surgical excision after changing in size and shape of the lesion. After surgical excision and histopathological examination, a diagnosis of syringocystadenoma papilliferum arising from a sebaceous mole was made. Conclusion: Syringocystadenoma papilliferum is a rare skin tumor. Histological confirmation was required for a definitive diagnosis. Furthermore, surgery and complete removal of the lesion are needed for treatment.
{"title":"SyringocystadenomaPapilliferum Arising from Nevus Sebaceous: A Case Report","authors":"T. Eslammanesh","doi":"10.52547/ajcm.27.4.261","DOIUrl":"https://doi.org/10.52547/ajcm.27.4.261","url":null,"abstract":"Background: Nevus sebaceous is a cutaneous hamartoma accompanied by secondary tumors, such as syringocystadenoma papilliferum. Syringocystadenoma papilliferum is a rare benign tumor with a controversial differentiation between eccrine and apocrine. Most syringocystadenoma papilliferum involving the scalp arises from a nevus sebaceous. Case Presentation: Herein, we report a 15-year-old adolescent female with a congenital skin lesion on the forehead undergoing surgical excision after changing in size and shape of the lesion. After surgical excision and histopathological examination, a diagnosis of syringocystadenoma papilliferum arising from a sebaceous mole was made. Conclusion: Syringocystadenoma papilliferum is a rare skin tumor. Histological confirmation was required for a definitive diagnosis. Furthermore, surgery and complete removal of the lesion are needed for treatment.","PeriodicalId":52678,"journal":{"name":"pzshkhy blyny bn syn","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46059251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kazem Farzinfar, H. Nejat, M. Doustkam, Hassan Tuzandeh Jani
Background and Objective: Numerous approaches, including schema therapy, and an integrated cognitive-speech approach, have been suggested to reduce emotions and increase distress tolerance in people with stuttering. The present study aimed to make a comparison between the effectiveness of schema therapy and integrated cognitive-speech approach in emotional regulation and distress tolerance of individuals suffering from stuttering. Materials and Methods: This quasi-experimental study was conducted based on a pre-test, post-test design, and a follow-up test with a control group. The statistical population included all individuals aged 18-30 who suffered from stuttering in Sabzevar city. The sample consisted of 30 people who were selected via convenience sampling and were randomly assigned to two experimental groups and one control group (n=10 in each group). The experimental schema therapy group underwent treatment in 12 90-minute sessions, the integrated cognitive-speech approach group received 12 60minute sessions of treatment, and the control group received no treatments. The instruments of the research were Gross and John (2003) Emotional Regulation Questionnaire, and Simons and Gaher (2005) Distress Tolerance Questionnaire. These instruments were conducted on three groups in all phases of pre-testing and post-testing and follow-up. The one-way repeated measures ANOVA and Tukey post hoc test were used to analyze the data. Results: As evidenced by the obtained results, the schema therapy and integrated cognitive-speech approach had a significant effect on emotional regulation and distress tolerance of individuals suffering from stuttering
背景与目的:包括图式疗法和认知-言语综合疗法在内的许多方法已被建议用于减少口吃患者的情绪和增加痛苦耐受性。本研究旨在比较图式疗法与认知-言语综合疗法在口吃患者情绪调节和痛苦耐受方面的效果。材料与方法:拟实验研究采用前测、后测和对照组随访设计。统计人群包括萨夫泽瓦尔市所有年龄在18-30岁的口吃患者。本研究采用方便抽样法选取30人，随机分为2个实验组和1个对照组(每组10人)。实验图式疗法组接受12个90分钟疗程的治疗，认知-言语综合疗法组接受12个60分钟疗程的治疗，对照组不接受任何治疗。研究工具为Gross and John(2003)的情绪调节问卷和Simons and Gaher(2005)的痛苦容忍问卷。这些仪器在前测、后测和随访的各个阶段对三组进行。采用单因素重复测量方差分析和Tukey事后检验对数据进行分析。结果:图式疗法和认知-言语整合疗法对口吃患者的情绪调节和痛苦耐受有显著影响
{"title":"Comparison of the Effectiveness of Schema Therapy and Integrated Cognitive-Speech Approach in Emotional Regulation, and Distress Tolerance of Individuals Suffering from Stuttering","authors":"Kazem Farzinfar, H. Nejat, M. Doustkam, Hassan Tuzandeh Jani","doi":"10.52547/ajcm.27.4.239","DOIUrl":"https://doi.org/10.52547/ajcm.27.4.239","url":null,"abstract":"Background and Objective: Numerous approaches, including schema therapy, and an integrated cognitive-speech approach, have been suggested to reduce emotions and increase distress tolerance in people with stuttering. The present study aimed to make a comparison between the effectiveness of schema therapy and integrated cognitive-speech approach in emotional regulation and distress tolerance of individuals suffering from stuttering. Materials and Methods: This quasi-experimental study was conducted based on a pre-test, post-test design, and a follow-up test with a control group. The statistical population included all individuals aged 18-30 who suffered from stuttering in Sabzevar city. The sample consisted of 30 people who were selected via convenience sampling and were randomly assigned to two experimental groups and one control group (n=10 in each group). The experimental schema therapy group underwent treatment in 12 90-minute sessions, the integrated cognitive-speech approach group received 12 60minute sessions of treatment, and the control group received no treatments. The instruments of the research were Gross and John (2003) Emotional Regulation Questionnaire, and Simons and Gaher (2005) Distress Tolerance Questionnaire. These instruments were conducted on three groups in all phases of pre-testing and post-testing and follow-up. The one-way repeated measures ANOVA and Tukey post hoc test were used to analyze the data. Results: As evidenced by the obtained results, the schema therapy and integrated cognitive-speech approach had a significant effect on emotional regulation and distress tolerance of individuals suffering from stuttering","PeriodicalId":52678,"journal":{"name":"pzshkhy blyny bn syn","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41674735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Objective: The changes that occur in the female body with the onset of menstruation affect the psychological components, including mental health. This study aimed to compare the mental health of adolescent females with early, late, and normal menarche. Materials and Methods: This descriptive study was conducted based on a causal-comparative design. The statistical population included 9to 17-yearold female students in Isfahan, Iran, who were selected through multistage random cluster sampling. The sample size was determined at 56, 37, and 43 cases in the group of students with normal, early, and late menarche, respectively. The data were collected using the Pediatric Symptom Checklist for school-aged children (age range: 3-18 years) (Jelink, Murphy, and Burns, 1986). Following that, the collected data were analyzed using SPSS software (version 25) through a covariance test. Results: According to the results, no significant difference was observed between the two groups of female students with normal and early menarche regarding mental health and its subscales. However, the adolescent females with late menarche had better mental health, compared to those with early and normal menarche (P<0.01). Conclusion: Based on the results, it seems that the timing of the onset of menstruation affects mental health. The late menarche is associated with fewer mental health problems.
{"title":"Comparison of Mental Health of Female Students with Early, Late, and Normal Menarche in Isfahan, Iran","authors":"Elham Irannezhad, M. Soltanizadeh","doi":"10.52547/ajcm.27.4.253","DOIUrl":"https://doi.org/10.52547/ajcm.27.4.253","url":null,"abstract":"Background and Objective: The changes that occur in the female body with the onset of menstruation affect the psychological components, including mental health. This study aimed to compare the mental health of adolescent females with early, late, and normal menarche. Materials and Methods: This descriptive study was conducted based on a causal-comparative design. The statistical population included 9to 17-yearold female students in Isfahan, Iran, who were selected through multistage random cluster sampling. The sample size was determined at 56, 37, and 43 cases in the group of students with normal, early, and late menarche, respectively. The data were collected using the Pediatric Symptom Checklist for school-aged children (age range: 3-18 years) (Jelink, Murphy, and Burns, 1986). Following that, the collected data were analyzed using SPSS software (version 25) through a covariance test. Results: According to the results, no significant difference was observed between the two groups of female students with normal and early menarche regarding mental health and its subscales. However, the adolescent females with late menarche had better mental health, compared to those with early and normal menarche (P<0.01). Conclusion: Based on the results, it seems that the timing of the onset of menstruation affects mental health. The late menarche is associated with fewer mental health problems.","PeriodicalId":52678,"journal":{"name":"pzshkhy blyny bn syn","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43432455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Objective: Adolescent idiopathic scoliosis is one of the complex and common deformities of the spine. It is a condition that can cause a lot of pain and discomfort for individuals and has consequences and complications. There are different exercises and approaches for correction of this deformity, such as Schroth exercise. In this regard, the purpose of this study was to investigate the effect of corrective exercises on Cobb angle, flexibility, functional capacity, and quality of life of adolescents with idiopathic scoliosis. Materials and Methods: This controlled clinical trial with pre-test and posttest design was performed on 24 adolescents with idiopathic scoliosis who were randomly divided into control and experimental groups. Cobb angle, flexibility, and functional capacity were measured by X-ray imaging, flexibility box, and six-minute walk test, respectively. The collected data were statistically analyzed in SPSS software (version 23) using a mixeddesign analysis of variance with repeated measures. It must be noted that a p-value of 0.05 was considered statistically significant. Results: Based on the results, after 10 weeks of Schroth exercises, there was a significant difference in the exercise group regarding the reduction of Cobb angle as well as the increase of flexibility and functional capacity. However, no significant difference was observed in the control group from pre-test to
{"title":"Effects of Specific Schroth Exercises on Cobb Angle, Flexibility, and Functional Capacity of Adolescents with Idiopathic Scoliosis","authors":"F. Saki, H. Gholami, A. Yalfani, Marziyeh Zia","doi":"10.52547/ajcm.27.4.217","DOIUrl":"https://doi.org/10.52547/ajcm.27.4.217","url":null,"abstract":"Background and Objective: Adolescent idiopathic scoliosis is one of the complex and common deformities of the spine. It is a condition that can cause a lot of pain and discomfort for individuals and has consequences and complications. There are different exercises and approaches for correction of this deformity, such as Schroth exercise. In this regard, the purpose of this study was to investigate the effect of corrective exercises on Cobb angle, flexibility, functional capacity, and quality of life of adolescents with idiopathic scoliosis. Materials and Methods: This controlled clinical trial with pre-test and posttest design was performed on 24 adolescents with idiopathic scoliosis who were randomly divided into control and experimental groups. Cobb angle, flexibility, and functional capacity were measured by X-ray imaging, flexibility box, and six-minute walk test, respectively. The collected data were statistically analyzed in SPSS software (version 23) using a mixeddesign analysis of variance with repeated measures. It must be noted that a p-value of 0.05 was considered statistically significant. Results: Based on the results, after 10 weeks of Schroth exercises, there was a significant difference in the exercise group regarding the reduction of Cobb angle as well as the increase of flexibility and functional capacity. However, no significant difference was observed in the control group from pre-test to","PeriodicalId":52678,"journal":{"name":"pzshkhy blyny bn syn","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45333476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Keramat, Hamid Reza Ghasemi Basir, A. Taher, A. Moradi, A. Saadatmand, Pooria Owji Nejad
Background and Objective: Acinetobacter infection is one of the most common nosocomial infections that its antibiotic resistance has increased in recent years. This study aimed to evaluate drug resistance to colistin in nosocomial infections with multidrug-resistant (MDR) Acinetobacter baumannii. Materials and Methods: This cross-sectional study was performed on 45 blood or body fluid culture samples collected from hospitalized patients at Sina Hospital, Hamadan, Iran, within 2018-2019. The Minimum Inhibitory Concentration (MIC) was determined using E-test trips and disk diffusion method to evaluate the resistance of Acinetobacter baumannii to the antibiotic colistin according to the guidelines of the Clinical and Laboratory Standards Institute. The collected data were analyzed in SPSS software
{"title":"Evaluation of Antibiotic Resistance to Colistin in Nosocomial Infections with Multidrug-Resistant Acinetobacter","authors":"F. Keramat, Hamid Reza Ghasemi Basir, A. Taher, A. Moradi, A. Saadatmand, Pooria Owji Nejad","doi":"10.52547/ajcm.27.4.211","DOIUrl":"https://doi.org/10.52547/ajcm.27.4.211","url":null,"abstract":"Background and Objective: Acinetobacter infection is one of the most common nosocomial infections that its antibiotic resistance has increased in recent years. This study aimed to evaluate drug resistance to colistin in nosocomial infections with multidrug-resistant (MDR) Acinetobacter baumannii. Materials and Methods: This cross-sectional study was performed on 45 blood or body fluid culture samples collected from hospitalized patients at Sina Hospital, Hamadan, Iran, within 2018-2019. The Minimum Inhibitory Concentration (MIC) was determined using E-test trips and disk diffusion method to evaluate the resistance of Acinetobacter baumannii to the antibiotic colistin according to the guidelines of the Clinical and Laboratory Standards Institute. The collected data were analyzed in SPSS software","PeriodicalId":52678,"journal":{"name":"pzshkhy blyny bn syn","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42025462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hamideh Moghimi, S. Borzouei, A. Zamani, Mahdi Behzad
Background and Objective: The increased response of T helper (Th) 22 cells might be involved in the pathogenesis of Type 2 Diabetes Mellitus (T2DM). The present study aimed to investigate the transcription factor of Th22 cells (aryl hydrocarbon receptor [AHR]) and interleukin 22 (IL-22) in CD4 T cells as well as the impact of oral empagliflozin treatment. The correlation between the aforementioned factors and clinical parameters was also determined among the patients. Materials and Methods: In this case-control study, 50 patients under the treatment of metformin and gliclazide were divided into two equal groups, including the patients receiving empagliflozin (EMPA) and not receiving empagliflozin (EMPA, as the control group). The peripheral blood CD4 T cells were isolated on the initiation day of the study and after 6 months of therapy, and the gene expression levels of AHR and IL-22 were evaluated by real-time polymerase chain reaction. In addition, the production of IL-22 after activation was measured using enzyme-linked immunosorbent assay. Results: The levels of fasting plasma glucose and hemoglobin A1c diminished in the EMPA group after empagliflozin therapy, compared to those reported at the baseline (initiation day; P<0.001 and P=0.04). The gene expression level of IL-22 and production of IL-22 significantly reduced after 6 months of empagliflozin therapy (P=0.011 and P=0.001). A significant positive correlation between IL-22 production and its gene expression with AHR as well as between fasting plasma glucose and hemoglobin A1c was observed in the EMPA patients after therapy (P˂0.05). Conclusion: In addition to antidiabetic effects, empagliflozin has antiinflammatory impacts on the immune system, especially on Th22 cell-
{"title":"Evaluation of the Effect of Empagliflozin Therapy on T Helper 22 Cell-Related Factors in Patients with Type 2 Diabetes Mellitus","authors":"Hamideh Moghimi, S. Borzouei, A. Zamani, Mahdi Behzad","doi":"10.29252/AJCM.27.4.193","DOIUrl":"https://doi.org/10.29252/AJCM.27.4.193","url":null,"abstract":"Background and Objective: The increased response of T helper (Th) 22 cells might be involved in the pathogenesis of Type 2 Diabetes Mellitus (T2DM). The present study aimed to investigate the transcription factor of Th22 cells (aryl hydrocarbon receptor [AHR]) and interleukin 22 (IL-22) in CD4 T cells as well as the impact of oral empagliflozin treatment. The correlation between the aforementioned factors and clinical parameters was also determined among the patients. Materials and Methods: In this case-control study, 50 patients under the treatment of metformin and gliclazide were divided into two equal groups, including the patients receiving empagliflozin (EMPA) and not receiving empagliflozin (EMPA, as the control group). The peripheral blood CD4 T cells were isolated on the initiation day of the study and after 6 months of therapy, and the gene expression levels of AHR and IL-22 were evaluated by real-time polymerase chain reaction. In addition, the production of IL-22 after activation was measured using enzyme-linked immunosorbent assay. Results: The levels of fasting plasma glucose and hemoglobin A1c diminished in the EMPA group after empagliflozin therapy, compared to those reported at the baseline (initiation day; P<0.001 and P=0.04). The gene expression level of IL-22 and production of IL-22 significantly reduced after 6 months of empagliflozin therapy (P=0.011 and P=0.001). A significant positive correlation between IL-22 production and its gene expression with AHR as well as between fasting plasma glucose and hemoglobin A1c was observed in the EMPA patients after therapy (P˂0.05). Conclusion: In addition to antidiabetic effects, empagliflozin has antiinflammatory impacts on the immune system, especially on Th22 cell-","PeriodicalId":52678,"journal":{"name":"pzshkhy blyny bn syn","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41574422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Objective: Long non-coding ribonucleic acid (lncRNA) has been identified as an important gene regulator and prognostic marker in various cancers. The present study aimed to investigate the effects of Nuclear Paraspeckle Assembly Transcript1 (NEAT1) gene knockout using Clustered Regularly Interspaced Short Palindromic Repeats-associated Protein 9 (CRISPR/Cas9) in PC-3 cell line. Materials and Methods: In this experimental study, recombinant plasmids carrying single guide RNA in the CRISPR system were designed and constructed. The prostate cancer PC3 cell lines were transfected by recombinant vectors, and apoptotic gene expression was evaluated using the quantitative polymerase chain reaction technique. Furthermore, annexin, MTT, and wound healing assays were applied for apoptosis, cell proliferation, and cell migration activities respectively. Results: Knockout of NEAT1 gene using CRISPR/Cas9 technique caused significant changes in the expression of apoptosis-related genes in a way that the expression of P21, BCL2, and BIRC5 genes decreased, while the expression of P53 and BAX increased. In addition, in the treatment group, the rate of cell proliferation and migration was reduced compared to those in the control group. Moreover, an increase was observed in the apoptosis rate in genetically modified cells compared to the control group. Conclusion: As an oncogene, NEAT1 plays an important role in tumorigenesis and its knockout reduces cell survival and migration and increases the apoptosis of cancer cells.
{"title":"Therapeutic Efficacy Analysis of lncRNA NEAT1 Gene Knockout and Apoptosis Induction in Prostate Cancer Cell Line Using CRISPR/Cas9","authors":"Nastaran Haghighi, A. Doosti, J. Kiani","doi":"10.52547/ajcm.28.1.49","DOIUrl":"https://doi.org/10.52547/ajcm.28.1.49","url":null,"abstract":"Background and Objective: Long non-coding ribonucleic acid (lncRNA) has been identified as an important gene regulator and prognostic marker in various cancers. The present study aimed to investigate the effects of Nuclear Paraspeckle Assembly Transcript1 (NEAT1) gene knockout using Clustered Regularly Interspaced Short Palindromic Repeats-associated Protein 9 (CRISPR/Cas9) in PC-3 cell line. Materials and Methods: In this experimental study, recombinant plasmids carrying single guide RNA in the CRISPR system were designed and constructed. The prostate cancer PC3 cell lines were transfected by recombinant vectors, and apoptotic gene expression was evaluated using the quantitative polymerase chain reaction technique. Furthermore, annexin, MTT, and wound healing assays were applied for apoptosis, cell proliferation, and cell migration activities respectively. Results: Knockout of NEAT1 gene using CRISPR/Cas9 technique caused significant changes in the expression of apoptosis-related genes in a way that the expression of P21, BCL2, and BIRC5 genes decreased, while the expression of P53 and BAX increased. In addition, in the treatment group, the rate of cell proliferation and migration was reduced compared to those in the control group. Moreover, an increase was observed in the apoptosis rate in genetically modified cells compared to the control group. Conclusion: As an oncogene, NEAT1 plays an important role in tumorigenesis and its knockout reduces cell survival and migration and increases the apoptosis of cancer cells.","PeriodicalId":52678,"journal":{"name":"pzshkhy blyny bn syn","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70683378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Word Recognition Score in White Noise in Patients with Cochlear Implant Prosthesis toward Patients with Hearing Aid","authors":"Seyede Faranak Emami","doi":"10.52547/ajcm.27.3.171","DOIUrl":"https://doi.org/10.52547/ajcm.27.3.171","url":null,"abstract":"","PeriodicalId":52678,"journal":{"name":"pzshkhy blyny bn syn","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46331084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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