Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.12
Ayoub Ibrahim Ali Al-Jumaily, H. M. Al-Rubaie
This study was conducted to study the prevalence of Neospora caninum in the local breed chickens in Al- Fallujah district, Iraq. One hundred and eighty (91 males and 89 females) were purchased from four regions (Al-Fallujah Center, Al-Shehabi, Al-Saglawia and Al-Karma ) for study the effects of sex, and months in the infection rate. Blood samples collected and sera were isolated for (c-ELISA) during the period from December 2018 to September 2019. The total infection rate was 9.4% and there was highly significant(P≤0.01) difference between the females 11.2% (10/89) and males 7.6% (7/91) infections. Regions of Al- Fallujah district showed highly significant (P≤0.01) difference in the infection rates that were ranged between4.5% (2/44) in Al-Fallujah Center to 14.5% ( 7/48) in Al-Shehabi. The highest infection rate was demonstrated in May 25% (6/24) compared with December, July and August that were recorded 5% (1/20) with significant(P≤0.01) difference, and no infection rate was recorded in the January and February months. In conclusion ( it is the first study that described the infection rate of N. caninum in the local breed chickens) in Al- Fallujah district, Iraq.
{"title":"Seroprevalence of Neospora caninum in Local Breed chickens in AL-Fallujah District, Iraq.","authors":"Ayoub Ibrahim Ali Al-Jumaily, H. M. Al-Rubaie","doi":"10.37940/ajvs.2019.12.2.12","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.12","url":null,"abstract":"This study was conducted to study the prevalence of Neospora caninum in the local breed chickens in Al- Fallujah district, Iraq. One hundred and eighty (91 males and 89 females) were purchased from four regions (Al-Fallujah Center, Al-Shehabi, Al-Saglawia and Al-Karma ) for study the effects of sex, and months in the infection rate. Blood samples collected and sera were isolated for (c-ELISA) during the period from December 2018 to September 2019. The total infection rate was 9.4% and there was highly significant(P≤0.01) difference between the females 11.2% (10/89) and males 7.6% (7/91) infections. Regions of Al- Fallujah district showed highly significant (P≤0.01) difference in the infection rates that were ranged between4.5% (2/44) in Al-Fallujah Center to 14.5% ( 7/48) in Al-Shehabi. The highest infection rate was demonstrated in May 25% (6/24) compared with December, July and August that were recorded 5% (1/20) with significant(P≤0.01) difference, and no infection rate was recorded in the January and February months. In conclusion ( it is the first study that described the infection rate of N. caninum in the local breed chickens) in Al- Fallujah district, Iraq.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70217991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.6
D. Moosa, Ahmed N. Flayyih, Omar M. Meshal, Haneen B. Mohammed Nouri, Dép
This study was conducted to diagnose of Babesia spp by using fluorescent microscope and light microscope, the study also included evaluating of Hemogram (RBC, HB, PCV, MCV, MCHC, Total Leukocyte Count, Differential Leukocyte Count) in cows infected with Babesia spp which cause piroplasmosis. The study included examination of 60 local breed Cows from many districts of Mosul city from the beginning of February 2018 until the end of July 2018, 17 were clinically infected with piroplasmosis and the infection was confirmed by using Giemsa and Acridine orange staining blood smears, and 10 cows were clinically intact and served as control. the results indicated that the percentage of the infection with Babesia spp was 28.33%, and the percentage of parasitemia ranged between (5-23%) with mean of (12.57%). The Results showed a statistical significant decrease (P<0.05) in RBC, Hb, and PCV values of diseased animals, Normocytic Normochromic type of anemia was found, the results also indicated a significant decrease in total Leukocyte count and neutrophil, with significant increase in lymphocytes number. the results also indicated non-significant changes in Eosinophil, Basophil and Monocyte numbers.
{"title":"Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed Cows.","authors":"D. Moosa, Ahmed N. Flayyih, Omar M. Meshal, Haneen B. Mohammed Nouri, Dép","doi":"10.37940/ajvs.2019.12.2.6","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.6","url":null,"abstract":"This study was conducted to diagnose of Babesia spp by using fluorescent microscope and light microscope, the study also included evaluating of Hemogram (RBC, HB, PCV, MCV, MCHC, Total Leukocyte Count, Differential Leukocyte Count) in cows infected with Babesia spp which cause piroplasmosis. The study included examination of 60 local breed Cows from many districts of Mosul city from the beginning of February 2018 until the end of July 2018, 17 were clinically infected with piroplasmosis and the infection was confirmed by using Giemsa and Acridine orange staining blood smears, and 10 cows were clinically intact and served as control. the results indicated that the percentage of the infection with Babesia spp was 28.33%, and the percentage of parasitemia ranged between (5-23%) with mean of (12.57%). The Results showed a statistical significant decrease (P<0.05) in RBC, Hb, and PCV values of diseased animals, Normocytic Normochromic type of anemia was found, the results also indicated a significant decrease in total Leukocyte count and neutrophil, with significant increase in lymphocytes number. the results also indicated non-significant changes in Eosinophil, Basophil and Monocyte numbers.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70218313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/AJVS.2019.12.1.13
Nawfal Mutlak
This study was carried out by selecting a total number of 17 Holstein Friesian cows divided into two main groups, the first group (4) cows as donors used to take the embryos from after artificial insemination and the second group (13) heifers cows as recipients for embryos. The same techniques were used on donor and recipient cows for the purpose of hormonal preparation in terms of instance synchronization, preparing recipient cows and superovulation for donor cows. Artificial insemination were done in field, and a nonsurgical flushing procedure embryos applied then graded. Donors reflected a good response to superovulation hormonal treatment. The total number of transferable embryos was 13 from four donors. Evaluation of all embryos was excellent quality. The pregnancy rate results for recipient cows was 61.5%. This result is an acceptable as compared to previous studies due to many factors, the study aim was taking into account all these factors, that can have an influence on these programs to obtain genetically improved animals in a shorter time and lower costs.
{"title":"The influence of Multiple Ovulation and Nonsurgical Embryo Transfer on pregnancy rate in Holstein Friesian Heifers","authors":"Nawfal Mutlak","doi":"10.37940/AJVS.2019.12.1.13","DOIUrl":"https://doi.org/10.37940/AJVS.2019.12.1.13","url":null,"abstract":"This study was carried out by selecting a total number of 17 Holstein Friesian cows divided into two main groups, the first group (4) cows as donors used to take the embryos from after artificial insemination and the second group (13) heifers cows as recipients for embryos. The same techniques were used on donor and recipient cows for the purpose of hormonal preparation in terms of instance synchronization, preparing recipient cows and superovulation for donor cows. Artificial insemination were done in field, and a nonsurgical flushing procedure embryos applied then graded. Donors reflected a good response to superovulation hormonal treatment. The total number of transferable embryos was 13 from four donors. Evaluation of all embryos was excellent quality. The pregnancy rate results for recipient cows was 61.5%. This result is an acceptable as compared to previous studies due to many factors, the study aim was taking into account all these factors, that can have an influence on these programs to obtain genetically improved animals in a shorter time and lower costs.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70217513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/AJVS.2019.12.1.15
Waad Khalaf, M. Hasan
The present study was arranged to investigate the existence of different species of Nematodes which infect the cattle thorough an examination of 484 faecal samples which were collected randomly from Mosul city and around regions of it, during the period from October 2011 to June 2012 and determination infection severity of them. The total percentage of infection with Nematodes was 23.34%. The significant variations in percentages of infection were recorded according to months of study. The highest percentage was in December 42.10% and the lowest in February at 2.63%. In this study were diagnosed 13 genera of Nematodes, most of them Haemonchus spp. and Ostertagia spp. In the estimation of infection severity with Nematodes, significant variations were showed among the light infection with both of moderate and severe infection and the highest percentage of light has accomplished to 67.25% and the lowest in moderate infection was 11.50%.
{"title":"Diagnostic Study of Nematodes in Cattle in Mosul city","authors":"Waad Khalaf, M. Hasan","doi":"10.37940/AJVS.2019.12.1.15","DOIUrl":"https://doi.org/10.37940/AJVS.2019.12.1.15","url":null,"abstract":"The present study was arranged to investigate the existence of different species of Nematodes which infect the cattle thorough an examination of 484 faecal samples which were collected randomly from Mosul city and around regions of it, during the period from October 2011 to June 2012 and determination infection severity of them. The total percentage of infection with Nematodes was 23.34%. The significant variations in percentages of infection were recorded according to months of study. The highest percentage was in December 42.10% and the lowest in February at 2.63%. In this study were diagnosed 13 genera of Nematodes, most of them Haemonchus spp. and Ostertagia spp. In the estimation of infection severity with Nematodes, significant variations were showed among the light infection with both of moderate and severe infection and the highest percentage of light has accomplished to 67.25% and the lowest in moderate infection was 11.50%.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70217175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.15
L. Younis, S. Hatif, Q. Aboud
This review was assigned to illustrate the melatonin (MLT) effect on reproduction in sheep (ewes). However, the pathway by which MLT charge the seasonal reproduction are imperfectly understood in sheep, the researchers consents that MLT regulates reproduction under influence of day length (photoperiod) to guarantee that birth happen in appropriate date of the year, it’s called neuro-endocrine process. This mechanism mediates by the pineal hormone (MLT). The major role of MLT in ewes is to translate the photo signals into endocrine pulses (gonadotropin-releasing hormone (GnRH) secretion) at the end of the retino-pineal pathway. In sheep, the MLT receptors distributed in premammillary hypothalamus (PMH), pituitary gland and Suprachiasmatic nucleus (SCN), therefore, many brain loci are participating for MLT pathways to modify the seasonal reproduction. Melatonin stimulates GnRH secretion through effect in different regions and neurons in hypothalamus such as a pre-mammillary nucleus, Arcuate and several factors like kisspeptin, RF-amide related peptide-3 (RFRP-3) and Tyrosine Hydroxylase (TH). In addition, its indirectly control prolactin (PRL) output via an effect on Tuberalin release, which is mediate the mechanism of MLT activity on pituitary PRL secretion and regulate his seasonal cyclicity. The alter in day length is the principle ecological factor that control the breeding in seasonal domestic animals. Several reproductive activities are related to short days and begin during autumn when the day becomes short and a decline in temperature (short-day breeder). While expanding in the duration of light lead to a cessation in reproduction activities during late winter and early spring. In conclusion, according to the major physiological role of MLT, it can be used in different aspects in ewes reproduction industry such as induce oestrus, increment the ovulation rate and In vitro embryo production.
{"title":"The Role of Melatonin in Ewes Reproduction: a Review","authors":"L. Younis, S. Hatif, Q. Aboud","doi":"10.37940/ajvs.2019.12.2.15","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.15","url":null,"abstract":"This review was assigned to illustrate the melatonin (MLT) effect on reproduction in sheep (ewes). However, the pathway by which MLT charge the seasonal reproduction are imperfectly understood in sheep, the researchers consents that MLT regulates reproduction under influence of day length (photoperiod) to guarantee that birth happen in appropriate date of the year, it’s called neuro-endocrine process. This mechanism mediates by the pineal hormone (MLT). The major role of MLT in ewes is to translate the photo signals into endocrine pulses (gonadotropin-releasing hormone (GnRH) secretion) at the end of the retino-pineal pathway. In sheep, the MLT receptors distributed in premammillary hypothalamus (PMH), pituitary gland and Suprachiasmatic nucleus (SCN), therefore, many brain loci are participating for MLT pathways to modify the seasonal reproduction. Melatonin stimulates GnRH secretion through effect in different regions and neurons in hypothalamus such as a pre-mammillary nucleus, Arcuate and several factors like kisspeptin, RF-amide related peptide-3 (RFRP-3) and Tyrosine Hydroxylase (TH). In addition, its indirectly control prolactin (PRL) output via an effect on Tuberalin release, which is mediate the mechanism of MLT activity on pituitary PRL secretion and regulate his seasonal cyclicity. The alter in day length is the principle ecological factor that control the breeding in seasonal domestic animals. Several reproductive activities are related to short days and begin during autumn when the day becomes short and a decline in temperature (short-day breeder). While expanding in the duration of light lead to a cessation in reproduction activities during late winter and early spring. In conclusion, according to the major physiological role of MLT, it can be used in different aspects in ewes reproduction industry such as induce oestrus, increment the ovulation rate and In vitro embryo production.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70217772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.3
K. A. Ahmed, D. O. Muhammed, S. M. Sadq
The current study was presented to conclude the influence of different ratio of pomegranate peel powder (PP) in feed of Karadi male lambs, they divided to 4 groups (0% as a control, 1% treatment 1, 2% treatment 2 and 4% as treatment 3), on scrotal circumference (SC), scrotum height (SH), body weight (BW) and blood serum testosterone concentration (T). In this study, sixteen (16) of Karadi male lambs used with 24.30 ± 0.40 kg weight and with 4-5 months old, all lambs have been randomly divided equally into four (4) groups and individually penned in a box for during 63 days. Results in our study showed that SC was significantly different and recorded higher 17.875±0.54 cm in group that fed on 1% PP, also SH recorded higher and significant different in group fed on 1% PP compared with other groups 10.000±0.32 cm. Average blood serum testosterone reduces in all groups compared to control group that fed on 0% of PP (11.418±0.15 n.mol/L). Final body weight in our study had significantly different (P < 0.05) between groups, 1% PP recorded higher weight (34.9 ± 0.58) kg. Each average daily gain, total body gain and initial body weight had not significant different in all groups. Correlation coefficient between SC, SH, blood serum testosterone concentration and final BW computed and ranged between (-0.397 to + 0.914).
{"title":"Influence of Different Feeding Ratio of Pomegranate Peels Powder on Testicular Dimensions, Body Weight and Testosterone Concentration in Karadi Male Lambs","authors":"K. A. Ahmed, D. O. Muhammed, S. M. Sadq","doi":"10.37940/ajvs.2019.12.2.3","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.3","url":null,"abstract":"The current study was presented to conclude the influence of different ratio of pomegranate peel powder (PP) in feed of Karadi male lambs, they divided to 4 groups (0% as a control, 1% treatment 1, 2% treatment 2 and 4% as treatment 3), on scrotal circumference (SC), scrotum height (SH), body weight (BW) and blood serum testosterone concentration (T). In this study, sixteen (16) of Karadi male lambs used with 24.30 ± 0.40 kg weight and with 4-5 months old, all lambs have been randomly divided equally into four (4) groups and individually penned in a box for during 63 days. Results in our study showed that SC was significantly different and recorded higher 17.875±0.54 cm in group that fed on 1% PP, also SH recorded higher and significant different in group fed on 1% PP compared with other groups 10.000±0.32 cm. Average blood serum testosterone reduces in all groups compared to control group that fed on 0% of PP (11.418±0.15 n.mol/L). Final body weight in our study had significantly different (P < 0.05) between groups, 1% PP recorded higher weight (34.9 ± 0.58) kg. Each average daily gain, total body gain and initial body weight had not significant different in all groups. Correlation coefficient between SC, SH, blood serum testosterone concentration and final BW computed and ranged between (-0.397 to + 0.914).","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70218251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.1
D. A. Abood, M. Dawood, L. Mohammed
The study was aimed to investigate the histological characteristics of the Tomcats accessory sex glands. A total of six indigenous Tom cats were used in this study. The samples of the ampulla of ductus deferens, prostate and bulbourethral glands were collected and fixed in 10% buffered formalin saline, and processed with routine paraffin technique. The tissue sections were stained by Hematoxyline & Eosin, Masson's trichrom and PAS stains. Results showed that the mucosa of ampulla has composed of tunica mucosa, muscularis, and adventitia. The tunica mucosa lined by mucous secreting cells which showed negative reaction for PAS stain and non-secreting cells which have intense reaction for PAS stain. The tunica muscularis composed of circular oriented smooth muscle fibers. The tunica adventia composed of dense irregular collagenous connective tissue. Prostate was large tubuloalveolar gland, surrounded the pelvic urethra; the gland consisted of very thick peri urethral tunica mucosa which lined with transitional epithelium. The prostatic lamina propria composed of collagen bundles, tubuloalveolar glands and manifested by many of like cavernous tissue wide veins. The prostate glandular epithelium was pseudo stratified columnar to low cuboidal which showed intense reaction for PAS stain. The muscularis of prostate composed of thick layer of skeletal muscle cells. The bulbourethral glands surrounded by a thick fibro-muscular capsule whose septa subdivided the gland into lobules. Each lobule composed of tubuloalveolar secretory units. The secretory units were lined by pseudo stratified to simple columnar epithelium supported by a thin layer of myoepithelial cells surrounds each alveolus. The cytoplasm of epithelial secretory units contained acidic secretory granules with Alcian blue (2.5pH) stains (fig.11, 12 & 13). It was concluded that tom cat's accessory glands has devoid of seminal vesicles and has well developed and functional prostate and bulbourethral glands.
{"title":"Histological Features of the Accessory Sex Gland of Indigenous Tom cat (Felis catus)","authors":"D. A. Abood, M. Dawood, L. Mohammed","doi":"10.37940/ajvs.2019.12.2.1","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.1","url":null,"abstract":"The study was aimed to investigate the histological characteristics of the Tomcats accessory sex glands. A total of six indigenous Tom cats were used in this study. The samples of the ampulla of ductus deferens, prostate and bulbourethral glands were collected and fixed in 10% buffered formalin saline, and processed with routine paraffin technique. The tissue sections were stained by Hematoxyline & Eosin, Masson's trichrom and PAS stains. Results showed that the mucosa of ampulla has composed of tunica mucosa, muscularis, and adventitia. The tunica mucosa lined by mucous secreting cells which showed negative reaction for PAS stain and non-secreting cells which have intense reaction for PAS stain. The tunica muscularis composed of circular oriented smooth muscle fibers. The tunica adventia composed of dense irregular collagenous connective tissue. Prostate was large tubuloalveolar gland, surrounded the pelvic urethra; the gland consisted of very thick peri urethral tunica mucosa which lined with transitional epithelium. The prostatic lamina propria composed of collagen bundles, tubuloalveolar glands and manifested by many of like cavernous tissue wide veins. The prostate glandular epithelium was pseudo stratified columnar to low cuboidal which showed intense reaction for PAS stain. The muscularis of prostate composed of thick layer of skeletal muscle cells. The bulbourethral glands surrounded by a thick fibro-muscular capsule whose septa subdivided the gland into lobules. Each lobule composed of tubuloalveolar secretory units. The secretory units were lined by pseudo stratified to simple columnar epithelium supported by a thin layer of myoepithelial cells surrounds each alveolus. The cytoplasm of epithelial secretory units contained acidic secretory granules with Alcian blue (2.5pH) stains (fig.11, 12 & 13). It was concluded that tom cat's accessory glands has devoid of seminal vesicles and has well developed and functional prostate and bulbourethral glands.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70217241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.11
M. R. A. Al-Dahan, A. Majeed, M. Abed
The study was conducted to investigate the effect of addition of zinc sulphate, cysteine, Prostaglandin F2α (PGF2α) and their combination to the diluted semen on semen characteristics of Holstein bulls after different periods of cooling. The study was conducted on Artificial insemination Center/ Directorate of Animal Resources/ Ministry of Agriculture at Abu-Graib at the west of Baghdad during the period of Aug. 2019 to the Dec. 2019. Seven Holstein bulls were used, Aged between 2.5- 3 years. Semen was collected via Artificial Vagina one ejaculate per a week for three months. Fresh semen was evaluated which were of bad grand pooled semen were divided into 5 parts. The first part (T1) only diluted semen (Tris) serve as a control. The 2nd part (T2) added 0.576 mmol/ ml of zinc sulphate to diluted semen. The 3rd part (T3) added 5 mmol/ ml of cysteine to diluted semen. The 4th part (T4) added 37.5 pg/ ml of PGF2α to diluted semen to diluted semen. The 5th part (T5) added a combination of previous components to the diluted semen. Then the semen evaluated at 5C° after 24, 48 and 72 hrs. The results showed that the addition of zinc sulphate on cooled diluted semen have no significant difference between different periods in the percent of sperm individual motility. Addition of zinc sulphate and cysteine showed a significant increase (P<0.05) in the percent of sperm liveability. The addition of a combination (zn sulphate, cysteine and PGF2α) showed a significant decrease (P<0.05) in sperm abnormalities percent especially at 5C°. The results also showed the addition of zinc sulphate and PGF2α increase the percent of HOST (Hypo-osmotic swelling test) of spermatozoa at 48 hrs. after cooling. zinc sulphate addition showed a significant increase (P<0.05) at 5C° during as comported with other cooled period in the HOST. The addition of zinc sulphate showed a significant increase in cell membrane integrity at 5C° cooled semen. It was concluded that addition of antioxidant and hormone might improve semen quality of Holstein bulls.
{"title":"Effect of Addition of Zn, Cysteine, PGF2α and their Combination on Holstein Bulls Cooled Semen in vitro","authors":"M. R. A. Al-Dahan, A. Majeed, M. Abed","doi":"10.37940/ajvs.2019.12.2.11","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.11","url":null,"abstract":"The study was conducted to investigate the effect of addition of zinc sulphate, cysteine, Prostaglandin F2α (PGF2α) and their combination to the diluted semen on semen characteristics of Holstein bulls after different periods of cooling. The study was conducted on Artificial insemination Center/ Directorate of Animal Resources/ Ministry of Agriculture at Abu-Graib at the west of Baghdad during the period of Aug. 2019 to the Dec. 2019. Seven Holstein bulls were used, Aged between 2.5- 3 years. Semen was collected via Artificial Vagina one ejaculate per a week for three months. Fresh semen was evaluated which were of bad grand pooled semen were divided into 5 parts. The first part (T1) only diluted semen (Tris) serve as a control. The 2nd part (T2) added 0.576 mmol/ ml of zinc sulphate to diluted semen. The 3rd part (T3) added 5 mmol/ ml of cysteine to diluted semen. The 4th part (T4) added 37.5 pg/ ml of PGF2α to diluted semen to diluted semen. The 5th part (T5) added a combination of previous components to the diluted semen. Then the semen evaluated at 5C° after 24, 48 and 72 hrs. The results showed that the addition of zinc sulphate on cooled diluted semen have no significant difference between different periods in the percent of sperm individual motility. Addition of zinc sulphate and cysteine showed a significant increase (P<0.05) in the percent of sperm liveability. The addition of a combination (zn sulphate, cysteine and PGF2α) showed a significant decrease (P<0.05) in sperm abnormalities percent especially at 5C°. The results also showed the addition of zinc sulphate and PGF2α increase the percent of HOST (Hypo-osmotic swelling test) of spermatozoa at 48 hrs. after cooling. zinc sulphate addition showed a significant increase (P<0.05) at 5C° during as comported with other cooled period in the HOST. The addition of zinc sulphate showed a significant increase in cell membrane integrity at 5C° cooled semen. It was concluded that addition of antioxidant and hormone might improve semen quality of Holstein bulls.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70217322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.37940/ajvs.2019.12.2.9
M. Shareef, T. Mohammed, Hani muneeb Alrawi
This study was conducted to investigate the effect of yeast (Saccharomyces cerevisiae) enhanced with Selenium or Zinc on some hematological characteristics in the Iraqi female goats. The seventeen adult female goats 2-2.5 years old with average body weight (bw) 37.5 ± 2.5 kg that have at least one previous birth were used. The animals were randomly divided in to three groups, group 1 have 5 goats, group 2 and 3 have six goats. all doesss were fed same ration. Animals of group 1 (G1) were let without treatment which considered as a control group, Animals of group 2 (G2) was given yeast enriched with selenium (0.03 g/kg/bw), While Animals of group 3 (G3) were given yeast enriched with zinc (0.2 g/kg/bw). The animals were treated by dissolving the samples in 30 ml of distilled water and administered orally for 45 days. Blood samples were collected from the jugular vein during first day (first period), twelfth day (second period) fort- fifth day (third period) to carrying out blood tests. The results showed was significant increase (P≤0.05) in the count of the red blood cells, hemoglobin concentration and Hematocrit of second and third groups as compared with first group. While the second group was significant increase in the number of white blood cells compared with the first and third groups. From the results, it could be concluded that there synergistic effect of Saccharomyces cerevisiae with selenium or zinc may be attributed to improve some hematological aspects in does.
{"title":"Effect of Yeast (Saccharomyces cerevisiae) Enhanced with Selenium or Zinc on the Hematological Characteristics in Iraqi Does","authors":"M. Shareef, T. Mohammed, Hani muneeb Alrawi","doi":"10.37940/ajvs.2019.12.2.9","DOIUrl":"https://doi.org/10.37940/ajvs.2019.12.2.9","url":null,"abstract":"This study was conducted to investigate the effect of yeast (Saccharomyces cerevisiae) enhanced with Selenium or Zinc on some hematological characteristics in the Iraqi female goats. The seventeen adult female goats 2-2.5 years old with average body weight (bw) 37.5 ± 2.5 kg that have at least one previous birth were used. The animals were randomly divided in to three groups, group 1 have 5 goats, group 2 and 3 have six goats. all doesss were fed same ration. Animals of group 1 (G1) were let without treatment which considered as a control group, Animals of group 2 (G2) was given yeast enriched with selenium (0.03 g/kg/bw), While Animals of group 3 (G3) were given yeast enriched with zinc (0.2 g/kg/bw). The animals were treated by dissolving the samples in 30 ml of distilled water and administered orally for 45 days. Blood samples were collected from the jugular vein during first day (first period), twelfth day (second period) fort- fifth day (third period) to carrying out blood tests. The results showed was significant increase (P≤0.05) in the count of the red blood cells, hemoglobin concentration and Hematocrit of second and third groups as compared with first group. While the second group was significant increase in the number of white blood cells compared with the first and third groups. From the results, it could be concluded that there synergistic effect of Saccharomyces cerevisiae with selenium or zinc may be attributed to improve some hematological aspects in does.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70219036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.37940/ajvs.2018.11.2.9
A. Al-taee, M. Alneema
This study was conducted to investigate the existence and intensity of coccidial species in Breeder farms in Al-Hamdania region. Thirty six samples from the intestinal content were collected. They were obtained from 6 farms located within Al-Hamdania region including the following suburban regions: (Karakosh, Manara, Bazgertan, Karkasha, Kaberly, Karamles) by obtaining 6 birds from each farms. The results revealed the prescience of four of Eimeria species in broilers these species were: Eimeria tenella; E.necatrix; E.mivati; E.mitis;.The total percentage of coccidiosis was 63.8% being the highest during February and March, 83.8%, the lowest during October and November 55.5%.Among the Eimerial species detected in our study, Eimeria tenella had the highest percentage of isolation in layers 61.97%, while the lowest were E.necatrix 7.04%.
{"title":"Incidence of Eimeria spp in chicken breeders in Al-Hamdania region","authors":"A. Al-taee, M. Alneema","doi":"10.37940/ajvs.2018.11.2.9","DOIUrl":"https://doi.org/10.37940/ajvs.2018.11.2.9","url":null,"abstract":"This study was conducted to investigate the existence and intensity of coccidial species in Breeder farms in Al-Hamdania region. Thirty six samples from the intestinal content were collected. They were obtained from 6 farms located within Al-Hamdania region including the following suburban regions: (Karakosh, Manara, Bazgertan, Karkasha, Kaberly, Karamles) by obtaining 6 birds from each farms. The results revealed the prescience of four of Eimeria species in broilers these species were: Eimeria tenella; E.necatrix; E.mivati; E.mitis;.The total percentage of coccidiosis was 63.8% being the highest during February and March, 83.8%, the lowest during October and November 55.5%.Among the Eimerial species detected in our study, Eimeria tenella had the highest percentage of isolation in layers 61.97%, while the lowest were E.necatrix 7.04%.","PeriodicalId":52734,"journal":{"name":"mjl@ lnbr ll`lwm lbyTry@","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44578152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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