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Risk assessment of some residues in milk and some dairy products 牛奶及乳制品中某些残留物的风险评估
Pub Date : 2018-12-01 DOI: 10.21608/jvmr.2017.43327
Saadia H. El- shinawy, Gamal M. Hassan, Fayza A. Sdeek, Dina M. Ismaiel
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引用次数: 0
Immunomodulatory effects of lector on chicks vaccinated with Mycoplasma gallisipticum inactivated vaccine 鸡支原体灭活疫苗免疫雏鸡的免疫调节作用
Pub Date : 2018-12-01 DOI: 10.21608/JVMR.2017.43324
Fawzy. R. El Seedy, S. Tamam, H. S. Hassan, Mona Mohamed
The experiment was designed to investigate the immunomodulating effect of lector50 on general health and immune response of broiler chicks to Mycoplasmagallisepticum vaccination in commercial broiler chicks. the obtained results reveledsignificantly higher effects on body weight ,bursal, and thymic index on lectortreated group of chickens, while no effects on spleen index. Also significantimprovement in total and differential leukocytic count as well as significantlyhigher antibody titer was detected by ELISA in lector 50 treated groups.
本试验旨在研究lector50对肉仔鸡整体健康状况和对商品肉仔鸡接种毒支原体疫苗免疫反应的调节作用。结果表明,提取物组对鸡的体重、法氏囊指数和胸腺指数有显著的影响,而对脾脏指数没有影响。经ELISA检测,50组总白细胞计数和差异白细胞计数明显改善,抗体滴度明显提高。
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引用次数: 0
Prevalence of fungal pathogens in broiler chickens and their environment 肉鸡真菌病原菌的流行及其生存环境
Pub Date : 2018-12-01 DOI: 10.21608/jvmr.2017.43316
I. Radwan, A. Abed, Athar S.Abdallah.
ARTICLE INFO Fungal diseases of poultry have become problematic as bacterial and viral diseases. This study was designed to investigate the prevalence of fungal agents in broiler chickens and their environment. The prevalence of fungal isolation from broiler chickens was 21.6% including 12.8% moulds and 8.8% yeast while the prevalence of fungal isolation from the environment was 46.8% including 25.5% moulds and 21.3% yeast. Aspergillus species was the most prevalent moulds while C. albicans was the most prevalent yeast recovered from broiler chickens and their environment. Article history:
家禽真菌病已成为与细菌性和病毒性疾病一样的问题。本试验旨在调查肉鸡及其生活环境中真菌病原的流行情况。肉鸡真菌分离率为21.6%(霉菌12.8%,酵母菌8.8%),环境真菌分离率为46.8%(霉菌25.5%,酵母菌21.3%)。从肉鸡及其环境中回收的真菌中,最常见的菌种是曲霉,而白色念珠菌是最常见的酵母菌。文章历史:
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引用次数: 3
Quality Evaluation Of Some Crustaceans In Beni-Suef Markets 惠州市场上部分甲壳类动物的质量评价
Pub Date : 2018-12-01 DOI: 10.21608/jvmr.2017.43321
Fatma H.M. Ali, Abdel- Rahim H.A. Hassan, Gehan M. Oaf, Ahmed A. Elmasry
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引用次数: 0
Estimation of Some Heavy Metals Concentration in Layer Farms at El-Fayoum Governorate 法尤姆省养鸡场某些重金属浓度的估计
Pub Date : 2018-12-01 DOI: 10.21608/jvmr.2017.43322
K. Abdou, W. Moselhy, M. Manal, O. H. Ehdaa
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引用次数: 4
A qualitative immunoassay as complementary test with tuberculin skin test for detection of tuberculosis in dairy cattle 用定性免疫分析法与结核菌素皮肤试验互补检测奶牛结核病
Pub Date : 2018-12-01 DOI: 10.21608/jvmr.2017.43317
Walid Hamdy Hassan, Essam Amin Nasr, Hassan Mohamed Moussa
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引用次数: 1
Some reproductive hormones in relation to ovarian activity in rats 一些生殖激素与大鼠卵巢活动的关系
Pub Date : 2018-12-01 DOI: 10.21608/JVMR.2017.43323
A. Aboul-Ela, A. El-Anwar, E. Mabrouk, A. Aboul-Khair
The present study is an endeavor to explore the relationship between induced hormonal alterations and ovarian activity in female rats. Fifty mature cycling female Albino rats were left for one week for acclimatization and offered balanced diet and water ad libitum. Animals were daily examined by vaginal smears to determine regularity of the estrous cycle. Rats were equally divided into 5 groups; control, hyperglycemic, hypoglycemic, hypercorticosteroid and hypocorticosteroid. At the end of 3 cycles, individual sera were obtained to determine glucose, malondialdehyde (MDA), estradiol and progesterone levels. Moreover, tissue specimens of the ovaries and the Fallopian tube were taken for histopathological examination. Results showed that all hormonal treatments induced alterations in the cellular characteristics of the cycle. Gonadosomaic index (GSI) did not show any remarkable variation. Alloxan or insulin treatments affected significantly serum glucose level in rats as. On the other side, hypercorticism led to marked elevation of glucose while hypocorticism showed no significant effect. Serum MDA showed significant elevation only in hyperglycemic and hypocorticoid groups. Hypoglycemia led to a significant decrease in serum estradiol while other treatments had no effect. Upon progesterone, hypoglycemia resulted in an increased level of the hormone while other treatments led to reduced levels of the hormone. It was evident that abnormalities of the adrenal corticosteroids and / or pancreatic insulin levels are concomitant with irregular estrous cycle as well as ovarian and Fallopian tube alterations which led to deviated gonadal folliculogenesis and steroidogenesis. ARTICLE INFO
本研究旨在探讨雌性大鼠诱导的激素变化与卵巢活动之间的关系。50只成熟循环雌性白化病大鼠适应环境1周,并给予均衡饮食和随意饮水。每天通过阴道涂片检查动物以确定发情周期的规律。大鼠平均分为5组;控制,高血糖,低血糖,高皮质类固醇和低皮质类固醇。3个周期结束时,取个体血清测定葡萄糖、丙二醛(MDA)、雌二醇和孕酮水平。并取卵巢、输卵管组织标本进行组织病理学检查。结果表明,所有激素治疗均可引起月经周期细胞特征的改变。促性腺指数(Gonadosomaic index, GSI)无明显变化。四氧嘧啶或胰岛素治疗显著影响大鼠血清葡萄糖水平。另一方面,高皮质导致葡萄糖显著升高,而低皮质无显著影响。血清MDA仅在高血糖和低皮质激素组有显著升高。低血糖导致血清雌二醇显著下降,而其他治疗没有效果。在黄体酮治疗中,低血糖导致激素水平升高,而其他治疗导致激素水平降低。很明显,肾上腺皮质激素和/或胰腺胰岛素水平的异常与不规则的发情周期以及卵巢和输卵管的改变同时发生,这些改变导致性腺卵泡发生和类固醇发生的偏差。条信息
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引用次数: 0
Beneficial roles of boron supplementation in the ration of rams on productive activity and semen quality 公羊日粮中添加硼对生产活性和精液质量的有益作用
Pub Date : 2018-12-01 DOI: 10.21608/JVMR.2017.43314
T. Ibrahim, A. Abdel-Wahab, A. El-Anwar, S. Ibrahim
The aim of this study is to determine the effects of boron supplementation in ration of rams on their productive activity and semen quality. For that purpose, twelve rams were divided into two groups (6 rams/ each). Rams of the first control group were fed the basal ration. Concerning the second group (boron group), the rams were fed the basal ration plus 400 mg boric acid (BA) / kg diet as a source of boron. The rams in both groups fed their corresponding ration for 4 months ad libitum. Blood samples were collected monthly throughout the experimental period. Semen samples were collected at the 12th and 16th weeks of the experiment. The results clarified that the serum levels of tetraiodotyrosine (T4) were observed to be elevated significantly (P<0.05) with growing of age in the rams fed B, but they didn't change in the control group. In addition, the semen quality was maintained normal with boronsupplementation as the individual sperm motilities and live/dead sperm cells percentages were similar to control. Also, boronsupplementation didn't induce any adverse effects on the serum levels of Mg when compared to control group. Thus, boronis considered a potential and useful supplement in ration of rams to improve the thyroid activities and didn't induce any adverse effects on either semen quality or serum magnesium level in rams.
本试验旨在探讨饲粮中添加硼对公羊生产活性和精液质量的影响。为此,将12只公羊分成两组(每组6只)。第一对照组饲喂基础日粮。第二组(硼组)在基础日粮基础上添加400 mg硼酸/ kg饲粮作为硼源。两组公羊分别饲喂相应日粮,自由饲喂4个月。在整个实验期间每月采集血液样本。在实验第12周和第16周采集精液样本。结果表明,B组羊血清四碘酪氨酸(T4)水平随着年龄的增长而显著升高(P<0.05),而对照组没有变化。此外,由于个体精子活力和活/死精子细胞百分比与对照组相似,补硼后精子质量保持正常。此外,与对照组相比,补硼对血清Mg水平没有任何不良影响。因此,在公羊日粮中添加硼酸钠可以提高甲状腺活性,且对公羊精液质量和血清镁水平均无不良影响。
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引用次数: 0
Seroprevalence and bacteriological identification of brucellosis in buffaloes in Upper Egypt. 上埃及水牛布鲁氏菌病的血清流行率和细菌学鉴定。
Pub Date : 2018-06-01 DOI: 10.21608/JVMR.2018.43312
M. Ragaa, F. El-Seedy, Abou-Gazia K.A
A total of 1317 samples were collected; 1164 serum samples, 122 milk samples, 24 lymph nodes and 7 aborted foeti from buffaloes in 10 Governorates from farms and villages in Upper Egypt. The serological tests used for the diagnosis of brucellosis on blood sera were the Rose Bengal plate (RBT) , Buffered acidified plate antigentest (BABAT), EDTA modifiedstandard tube agglutination test (MSAT), Revanol test (RT). On the other hand, the milk ring test (MRT) was performed on buffalo-cow's milk. Suspected colonies were stained with Gram, s stain and Modified ZeilNeelson stain. The isolated Brucella organisms on antibiotic free Brucella agar medium were subjected to the following tests for biochemical identification tests as CO2requirement, H2S production, Urease activity, growth in the presence of dyes,The indirect solid phase ELISA technique was carried out according to serum and milk samples.Agar gel immune diffusion test (AGID) and PCR applied on isolated Brucella strains. The results of the serological tests wereRose Bengal test 34.7%, BAPA (37%), Revanol test (28.2%),modified SAT (23.7%), indirect ELISAwere (32.3%) and AGPT (33.8%)in this study.Brucellaorganisms from lymph nodes of slaughtered buffaloes by culturing method showed that 3 (13.64%) isolates(2) of B. melitensisbiovar 3 and (1)B. abortusbiovar1. The isolated strain from aborted foeti was one isolate (14.29%) typed as B.melitensisbiovar 3. isolated only from Beni-Suef.By milk ring test (MRT) milk samples were 10 (8.20%) of B. melitensis biotype 3. A multiplex wasformat that will allow the rapid identification of Brucella spp., B. abortus,and B.melitensis in a single test within 2 to 3 h. B. melitensis was identifiedat 731bp and B. abortus identified at 498bp. Finally, we made measures ofthe control program for eradication of brucellosis in buffaloes by areasonable system of compensation, Veterinarians for field work and statelaboratories capable of serological techniques.Also, information technologysolutions and further logistic means as animal identification techniques are inmany governorates in Egypt.
共采集样本1317份;在上埃及10个省的农场和村庄采集了1164个血清样本、122个牛奶样本、24个淋巴结和7个流产的水牛胎儿。用于诊断布鲁氏菌病的血清学试验有玫瑰孟加拉平板(RBT)、缓冲酸化平板抗原性(BABAT)、EDTA改良标准管凝集试验(MSAT)、Revanol试验(RT)。另一方面,对水牛乳进行乳环试验(MRT)。用革兰氏染色和改良ZeilNeelson染色对可疑菌落进行染色。将分离得到的布鲁氏菌在不含抗生素的布鲁氏菌琼脂培养基上进行co2需取量、H2S产量、脲酶活性、染料存在下的生长等生化鉴定试验,并根据血清和牛奶样品进行间接固相酶联免疫吸附试验。琼脂凝胶免疫扩散试验(AGID)和PCR技术在布鲁氏菌分离株中的应用。血清学检测结果为:孟加拉试验34.7%,BAPA (37%), Revanol试验(28.2%),改良SAT(23.7%),间接elisa (32.3%), AGPT(33.8%)。用培养法从屠宰水牛淋巴结中分离出布鲁氏菌3株(占13.64%);abortusbiovar1。从流产胎中分离到的菌株1株(14.29%)分型为melitensisbiovar 3。仅从Beni-Suef中分离。乳环试验(MRT)结果显示,10份乳样品为3型白耳白杆菌,占8.20%。多重检测格式可在2 - 3小时内一次快速鉴定布鲁氏菌、B. abortus和B.melitensis。B.melitensis在731bp处鉴定,B. abortus在498bp处鉴定。最后,通过合理的补偿制度、兽医的现场工作和具备血清学技术的国家实验室,制定了根除水牛布鲁氏菌病的控制方案。此外,信息技术解决方案和进一步的物流手段,如动物识别技术,在埃及的许多省份。
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引用次数: 0
Bacterial pathogens associated with cellulitis in chickens 与鸡蜂窝织炎有关的细菌病原体
Pub Date : 2018-06-01 DOI: 10.21608/jvmr.2018.43303
I. Radwan, A. Abed, M. A. Allah, A. M.A.A.
ARTICLE INFO Cellulitis is a serious problem for the poultry industry because of increased condemnations, carcass downgrading at processing, and higher labor costs to process affected flocks. In the present study, the prevalence of cellulitis was studied in 240 broiler chickens. The correlation between cellulitis and other systemic lesions of the same bird was investigated also. Moreover, identification of the causative bacterial agents was conducted focusing on E. coli and Salmonella isolates. The prevalence rate of cellulitis in examined broiler chickens was 38.3%. Cellulitis without systemic lesion was observed in 14.2% of birds while 24.2% of birds had cellulitis associated with other systemic lesions in the internal organs while hepatitis was the most frequent. The bacteriological examination revealed that of 253 samples collected, a total of 157 bacterial isolates were recovered (62.1%). Among the recovered isolates, E. coli was the most prevalent (126 isolates; 80.3%) as well as 4 Salmonella species (2.5%), 9 Proteus species (5.7%), 7 Pseudomonas aeruginosa (4.5%), 3 Enterobacter species (1.9%) and 8 Staphylococcus aureus (5.1%). Serogrouping of E. coli isolates revealed that O125 was the most prevalent; 32%, followed by serogroups O158, O55, O78 as 24%, 12%, 10%, respectively, then both O1 and O8; 6% for each, and finally O15; 4%. Antibiogram of E. coli isolates showed a high sensitivity against enrofloxacin only (81%) while they were moderately sensitive to apramycin (65.9%) and colistin sulphate (61.9%) as well as ciprofloxacin and cefotaxime sodium (56.3% and 55.6%, respectively). On the other hand, high moderate degrees of resistances were observed against the other antimicrobials. Salmonella isolates showed complete sensitivities to ciprofloxacin and enrofloxacin while they were completely resistant to most of antimicrbials. Article history:
蜂窝织炎是家禽业的一个严重问题,因为谴责增加,加工时胴体降级,以及处理受影响禽群的劳动力成本增加。本研究对240只肉鸡蜂窝织炎的患病率进行了研究。蜂窝织炎与其他系统性病变的相关性也进行了研究。此外,还对大肠杆菌和沙门氏菌进行了病原菌鉴定。被测肉鸡蜂窝织炎患病率为38.3%。14.2%的鸟类蜂窝织炎无全身性病变,24.2%的鸟类蜂窝织炎伴其他内脏全身性病变,以肝炎最为常见。细菌学检查结果:253份标本中,检出细菌157株(62.1%)。检出菌株中以大肠杆菌最多(126株);沙门氏菌4种(2.5%)、变形杆菌9种(5.7%)、铜绿假单胞菌7种(4.5%)、肠杆菌3种(1.9%)、金黄色葡萄球菌8种(5.1%)。大肠杆菌血清分型结果显示,O125最常见;32%,其次为O158、O55、O78血清组,分别为24%、12%、10%,其次为O1和O8血清组;各占6%,最后占15%;4%。大肠杆菌对恩诺沙星有较高的敏感性(81%),对阿帕霉素(65.9%)和硫酸粘菌素(61.9%)、环丙沙星和头孢噻肟钠有中等敏感性(56.3%和55.6%)。另一方面,观察到对其他抗菌素的高中等程度的耐药性。分离的沙门氏菌对环丙沙星和恩诺沙星完全敏感,而对大多数抗菌药物完全耐药。文章历史:
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引用次数: 5
期刊
Journal of Veterinary Medical Research
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